LOCUS       DACDEA010000001       125496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDEA010000001 DACDEA010000000
VERSION     DACDEA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 125496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 125496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.001"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV136_000001"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV136_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8464024.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV136_000002"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV136_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8464025.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV136_000003"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV136_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8464026.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV136_000004"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV136_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8464027.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV136_000005"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV136_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8464028.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV136_000006"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV136_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8464029.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV136_000007"
     CDS             complement(8053..8523)
                     /locus_tag="IV136_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8464030.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV136_000008"
     CDS             complement(8569..8994)
                     /locus_tag="IV136_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8464031.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV136_000009"
     CDS             complement(9061..9330)
                     /locus_tag="IV136_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8464032.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV136_000010"
     CDS             complement(9341..10936)
                     /locus_tag="IV136_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8464033.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV136_000011"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV136_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8464034.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV136_000012"
     CDS             complement(14489..15049)
                     /locus_tag="IV136_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8464035.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV136_000013"
     CDS             15402..16376
                     /locus_tag="IV136_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8464036.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV136_000014"
     CDS             16546..17946
                     /locus_tag="IV136_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8464037.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV136_000015"
     CDS             complement(18024..18746)
                     /locus_tag="IV136_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8464038.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV136_000016"
     CDS             complement(18825..19331)
                     /locus_tag="IV136_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8464039.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV136_000017"
     CDS             19454..21253
                     /locus_tag="IV136_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8464040.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV136_000018"
     CDS             complement(21321..22895)
                     /locus_tag="IV136_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464041.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV136_000019"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV136_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8464042.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV136_000020"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV136_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8464043.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV136_000021"
     CDS             complement(24672..25310)
                     /locus_tag="IV136_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8464044.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV136_000022"
     CDS             complement(25311..25961)
                     /locus_tag="IV136_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8464045.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV136_000023"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV136_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8464046.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV136_000024"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV136_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8464047.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV136_000025"
     CDS             complement(29104..29844)
                     /locus_tag="IV136_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8464048.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV136_000026"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV136_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8464049.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV136_000027"
     CDS             complement(31215..32153)
                     /locus_tag="IV136_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8464050.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV136_000028"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV136_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8464051.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV136_000029"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV136_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8464052.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV136_000030"
     CDS             complement(35313..35618)
                     /locus_tag="IV136_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8464053.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV136_000031"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV136_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8464054.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV136_000032"
     CDS             complement(36724..37008)
                     /locus_tag="IV136_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464055.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV136_000033"
     CDS             complement(37026..37916)
                     /locus_tag="IV136_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8464056.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV136_000034"
     CDS             complement(37936..38154)
                     /locus_tag="IV136_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464057.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV136_000035"
     CDS             complement(38124..39824)
                     /locus_tag="IV136_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8464058.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IV136_000036"
     CDS             40091..40864
                     /locus_tag="IV136_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464059.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV136_000037"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV136_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8464060.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IV136_000038"
     CDS             complement(41688..42659)
                     /locus_tag="IV136_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8464061.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV136_000039"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV136_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8464062.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV136_000040"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV136_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8464063.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IV136_000041"
     CDS             complement(45614..46438)
                     /locus_tag="IV136_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8464064.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IV136_000042"
     CDS             complement(46411..47091)
                     /locus_tag="IV136_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8464065.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IV136_000043"
     CDS             complement(47114..48052)
                     /locus_tag="IV136_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8464066.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IV136_000044"
     CDS             complement(48297..49148)
                     /locus_tag="IV136_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8464067.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV136_000045"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV136_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8464068.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IV136_000046"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IV136_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8464069.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV136_000047"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV136_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8464070.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV136_000048"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV136_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8464071.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IV136_000049"
     CDS             complement(56026..57267)
                     /locus_tag="IV136_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8464072.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV136_000050"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV136_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8464073.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IV136_000051"
     CDS             complement(58294..59178)
                     /locus_tag="IV136_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8464074.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IV136_000052"
     CDS             complement(59956..61242)
                     /locus_tag="IV136_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8464075.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IV136_000053"
     CDS             complement(61235..62503)
                     /locus_tag="IV136_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8464076.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IV136_000054"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IV136_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8464077.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="IV136_000055"
     CDS             complement(63275..63721)
                     /locus_tag="IV136_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464078.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="IV136_000056"
     CDS             complement(63814..64059)
                     /locus_tag="IV136_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464079.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="IV136_000057"
     CDS             complement(64174..64752)
                     /locus_tag="IV136_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8464080.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="IV136_000058"
     CDS             complement(64829..65359)
                     /locus_tag="IV136_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8464081.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV136_000059"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV136_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8464082.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="IV136_000060"
     CDS             complement(66216..66611)
                     /locus_tag="IV136_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8464083.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="IV136_000061"
     CDS             complement(66608..68020)
                     /locus_tag="IV136_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8464084.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV136_000062"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV136_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8464085.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68875..70335)
                     /locus_tag="IV136_000063"
     CDS             complement(68875..70335)
                     /locus_tag="IV136_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8464086.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="IV136_000064"
     CDS             complement(70505..71662)
                     /locus_tag="IV136_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8464087.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV136_000065"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV136_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8464088.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="IV136_000066"
     CDS             complement(73129..73656)
                     /locus_tag="IV136_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8464089.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="IV136_000067"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="IV136_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8464090.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="IV136_000068"
     CDS             74689..75936
                     /locus_tag="IV136_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8464091.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="IV136_000069"
     CDS             76012..76356
                     /locus_tag="IV136_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464092.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="IV136_000070"
     CDS             complement(76473..76799)
                     /locus_tag="IV136_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464093.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="IV136_000071"
     CDS             complement(77414..78616)
                     /locus_tag="IV136_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8464094.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="IV136_000072"
     CDS             78733..79248
                     /locus_tag="IV136_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8464095.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="IV136_000073"
     CDS             complement(79447..79893)
                     /locus_tag="IV136_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8464096.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV136_000074"
     CDS             complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV136_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8464097.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="IV136_000075"
     CDS             complement(81772..82401)
                     /locus_tag="IV136_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8464098.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="IV136_000076"
     CDS             complement(82385..82849)
                     /locus_tag="IV136_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8464099.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="IV136_000077"
     CDS             complement(83336..84604)
                     /locus_tag="IV136_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8464100.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="IV136_000078"
     CDS             complement(84835..85572)
                     /locus_tag="IV136_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8464101.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86207)
                     /locus_tag="IV136_000079"
                     /pseudo
     CDS             complement(85639..86207)
                     /locus_tag="IV136_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            86335..87207
                     /locus_tag="IV136_000080"
     CDS             86335..87207
                     /locus_tag="IV136_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8464102.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87549..88421
                     /locus_tag="IV136_000081"
     CDS             87549..88421
                     /locus_tag="IV136_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8464103.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88418..90376
                     /locus_tag="IV136_000082"
     CDS             88418..90376
                     /locus_tag="IV136_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464104.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90708..91559)
                     /locus_tag="IV136_000083"
     CDS             complement(90708..91559)
                     /locus_tag="IV136_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8464105.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91682..92149
                     /locus_tag="IV136_000084"
     CDS             91682..92149
                     /locus_tag="IV136_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8464106.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92412..92894)
                     /locus_tag="IV136_000085"
     CDS             complement(92412..92894)
                     /locus_tag="IV136_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8464107.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92994..93857)
                     /locus_tag="IV136_000086"
     CDS             complement(92994..93857)
                     /locus_tag="IV136_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8464108.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94455..95330
                     /locus_tag="IV136_000087"
     CDS             94455..95330
                     /locus_tag="IV136_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464109.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95475..95627)
                     /locus_tag="IV136_000088"
                     /pseudo
     CDS             complement(95475..95627)
                     /locus_tag="IV136_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(95895..97226)
                     /locus_tag="IV136_000089"
     CDS             complement(95895..97226)
                     /locus_tag="IV136_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8464110.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(97375..98088)
                     /locus_tag="IV136_000090"
     CDS             complement(97375..98088)
                     /locus_tag="IV136_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8464111.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            98263..99687
                     /locus_tag="IV136_000091"
     CDS             98263..99687
                     /locus_tag="IV136_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8464112.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            99986..100693
                     /locus_tag="IV136_000092"
     CDS             99986..100693
                     /locus_tag="IV136_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8464113.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            100680..101003
                     /locus_tag="IV136_000093"
     CDS             100680..101003
                     /locus_tag="IV136_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8464114.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(101092..102129)
                     /locus_tag="IV136_000094"
     CDS             complement(101092..102129)
                     /locus_tag="IV136_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8464115.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(102450..103433)
                     /locus_tag="IV136_000095"
     CDS             complement(102450..103433)
                     /locus_tag="IV136_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8464116.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(103848..104618)
                     /locus_tag="IV136_000096"
     CDS             complement(103848..104618)
                     /locus_tag="IV136_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464117.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(104611..105546)
                     /locus_tag="IV136_000097"
     CDS             complement(104611..105546)
                     /locus_tag="IV136_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464118.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(105862..107511)
                     /locus_tag="IV136_000098"
     CDS             complement(105862..107511)
                     /locus_tag="IV136_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8464119.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(107628..110231)
                     /locus_tag="IV136_000099"
     CDS             complement(107628..110231)
                     /locus_tag="IV136_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8464120.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(110243..111292)
                     /locus_tag="IV136_000100"
     CDS             complement(110243..111292)
                     /locus_tag="IV136_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8464121.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(111318..111632)
                     /locus_tag="IV136_000101"
     CDS             complement(111318..111632)
                     /locus_tag="IV136_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8464122.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(111648..112094)
                     /locus_tag="IV136_000102"
     CDS             complement(111648..112094)
                     /locus_tag="IV136_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8464123.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(112095..114071)
                     /locus_tag="IV136_000103"
     CDS             complement(112095..114071)
                     /locus_tag="IV136_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8464124.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(114229..115125)
                     /locus_tag="IV136_000104"
     CDS             complement(114229..115125)
                     /locus_tag="IV136_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8464125.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(115118..115798)
                     /locus_tag="IV136_000105"
     CDS             complement(115118..115798)
                     /locus_tag="IV136_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464126.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(115799..116581)
                     /locus_tag="IV136_000106"
     CDS             complement(115799..116581)
                     /locus_tag="IV136_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8464127.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(116574..117491)
                     /locus_tag="IV136_000107"
     CDS             complement(116574..117491)
                     /locus_tag="IV136_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464128.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(117655..117978)
                     /locus_tag="IV136_000108"
     CDS             complement(117655..117978)
                     /locus_tag="IV136_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8464129.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            118766..121993
                     /gene="fss3"
                     /locus_tag="IV136_000109"
     CDS             118766..121993
                     /gene="fss3"
                     /locus_tag="IV136_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8464130.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            122106..122420
                     /locus_tag="IV136_000110"
     CDS             122106..122420
                     /locus_tag="IV136_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8464131.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            122433..122807
                     /locus_tag="IV136_000111"
     CDS             122433..122807
                     /locus_tag="IV136_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8464132.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            122817..123152
                     /locus_tag="IV136_000112"
     CDS             122817..123152
                     /locus_tag="IV136_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8464133.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            123235..124575
                     /locus_tag="IV136_000113"
     CDS             123235..124575
                     /locus_tag="IV136_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8464134.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            124653..125327
                     /locus_tag="IV136_000114"
     CDS             124653..125327
                     /locus_tag="IV136_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8464135.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT        36062 a        26370 c        22450 g        40614 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctggcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tggaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct cattttaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gtgcattcgc tgcaacaatg
    85741 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    85801 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    85861 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    85921 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    85981 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    86041 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    86101 gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag cggatccgtt
    86161 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    86221 tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa catacttaac
    86281 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    86341 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    86401 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    86461 cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga ttctggtcga
    86521 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    86581 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    86641 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    86701 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    86761 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    86821 gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac agtcatccat
    86881 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    86941 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    87001 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    87061 atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc attacaagct
    87121 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    87181 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    87241 acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc ttaatttgtt
    87301 ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    87361 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    87421 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    87481 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    87541 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    87601 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    87661 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    87721 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    87781 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    87841 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    87901 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    87961 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    88021 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    88081 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    88141 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    88201 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    88261 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    88321 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    88381 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    88441 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    88501 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    88561 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    88621 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    88681 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    88741 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    88801 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    88861 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    88921 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    88981 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    89041 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    89101 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    89161 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    89221 caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct ttactggtgt
    89281 aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    89341 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    89401 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    89461 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    89521 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    89581 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    89641 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    89701 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    89761 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    89821 tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac aaacaaacac
    89881 cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt acgctatcgc
    89941 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    90001 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    90061 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    90121 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    90181 tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    90241 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    90301 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    90361 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    90421 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    90481 ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt atttgactct
    90541 tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg ttcaaaaacc
    90601 agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt
    90661 ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta ttcctgatcc
    90721 aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac tttttctaaa
    90781 tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc taaaaacatg
    90841 tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc tgcatcactt
    90901 gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc taattcttca
    90961 tagtcagtta gattgccgga taattctaag atttctggag agtcactttc tactagtact
    91021 tttgctgcag cttctgcata ttcacgtttc aatgcccaac ctgtttttcc atttcctgca
    91081 gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc attctctaca
    91141 taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat tttttcagtg
    91201 aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa acttgtataa
    91261 gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg ttcggcttga
    91321 cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc
    91381 attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc tttcagtgct
    91441 tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac catctttttt
    91501 aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa ataatgcata
    91561 tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt ataaattaca
    91621 cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag gatgacaact
    91681 gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata ttgaaatgtt
    91741 tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa caaatgcagc
    91801 aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg
    91861 aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag atgtctatcg
    91921 cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc caaattgcgt
    91981 cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc agaaaaaggc
    92041 agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag ccgttttgga
    92101 aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat tctctctact
    92161 gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc cgggggacaa
    92221 aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg aagtcatgac
    92281 gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa aatgcaaaat
    92341 atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct actttcacga
    92401 cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat atatttcccg
    92461 ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta atcttctact
    92521 tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat
    92581 tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat tcgggtagtg
    92641 tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc gttatagggt
    92701 tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc tattgttcca
    92761 ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta
    92821 ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt ttcttcgatc
    92881 ctatactttg ccatttctcc caccctttct attatattaa gttcatctta cataaaatta
    92941 aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt taactatttt
    93001 tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc tccccatttt
    93061 gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag ccattctgca
    93121 tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg
    93181 gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt ttcttttgtc
    93241 gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc tccatcaaat
    93301 ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg catctcttcc
    93361 ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac tccttcaaat
    93421 ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt ttcgatacga
    93481 aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt
    93541 tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg aaaagctctt
    93601 gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac tcttgatcca
    93661 tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc cattagcgac
    93721 atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc tgtattttcg
    93781 gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc gatcaattgg
    93841 ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat attatcaaag
    93901 aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa tgttgtatgg
    93961 atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat ctgcctatct
    94021 atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa acaaagcggt
    94081 aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa agcaaattgt
    94141 tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat catacctctt
    94201 ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt cagataaata
    94261 aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc tcacattctc
    94321 taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt atcaaatatg
    94381 taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata agtgtttcat
    94441 gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg
    94501 gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt
    94561 ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca
    94621 acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg
    94681 tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct
    94741 cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta
    94801 tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat gcaataaatt
    94861 attcagataa attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa
    94921 acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat
    94981 cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta
    95041 ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact
    95101 gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt
    95161 tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcca
    95221 taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag
    95281 ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
    95341 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
    95401 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
    95461 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta gattttccgg
    95521 cattgttgat agattttatg atacttttct acattttgta agagaggttc cactgcatct
    95581 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc taatctttaa
    95641 agataaatat taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa
    95701 cctatctttg catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat
    95761 aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata
    95821 tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt
    95881 tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt
    95941 cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct
    96001 gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga
    96061 atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa
    96121 gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat
    96181 ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag
    96241 tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt
    96301 cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt
    96361 tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct
    96421 aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata
    96481 gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact
    96541 atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat
    96601 aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca
    96661 gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata
    96721 aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta
    96781 taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac
    96841 atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata
    96901 tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac aactaataat
    96961 gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat
    97021 gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata
    97081 agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa
    97141 gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca
    97201 atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta
    97261 ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt aattaaatat
    97321 atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt
    97381 ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc
    97441 gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg
    97501 atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg
    97561 cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa
    97621 atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta
    97681 tggttgacaa tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat
    97741 acttaattta tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga
    97801 atctacgtgt tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga
    97861 gttatcaatt ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc
    97921 ttcggaaaca agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac
    97981 tgccaattca tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt
    98041 tttctttata tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc
    98101 aatcaaaaac atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa
    98161 aattatacac acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt
    98221 aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag
    98281 ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga
    98341 aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac
    98401 cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa
    98461 gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca
    98521 cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga
    98581 aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt
    98641 gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct
    98701 tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt
    98761 accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca
    98821 aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca
    98881 aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat
    98941 ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct
    99001 gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag
    99061 cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt
    99121 gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc
    99181 gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc
    99241 gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa
    99301 aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt
    99361 atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa
    99421 ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca
    99481 ataaatgaag gatgtaacat taaggggtat catctttgga catttattga ttgctggtca
    99541 tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa
    99601 agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga
    99661 tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta
    99721 gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata
    99781 cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc
    99841 taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt
    99901 tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt
    99961 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
   100021 agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg
   100081 gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg
   100141 ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt
   100201 tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
   100261 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
   100321 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
   100381 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
   100441 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
   100501 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
   100561 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
   100621 taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
   100681 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
   100741 ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt
   100801 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
   100861 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
   100921 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc
   100981 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
   101041 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
   101101 gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc
   101161 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
   101221 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
   101281 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
   101341 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
   101401 tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
   101461 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
   101521 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
   101581 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc
   101641 gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt
   101701 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc
   101761 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt
   101821 gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
   101881 cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc
   101941 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
   102001 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
   102061 gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt
   102121 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt
   102181 tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta
   102241 aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa
   102301 cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat
   102361 atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga
   102421 cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct
   102481 ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga
   102541 actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt
   102601 cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga
   102661 agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga
   102721 ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct
   102781 tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt
   102841 tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc
   102901 tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg
   102961 atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc
   103021 aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag
   103081 ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt
   103141 ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct
   103201 ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca
   103261 ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca
   103321 tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt
   103381 ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg
   103441 ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata
   103501 ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa
   103561 aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc
   103621 ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc
   103681 tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa
   103741 tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt
   103801 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat
   103861 cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca
   103921 agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa
   103981 ccgaagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac
   104041 atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc
   104101 acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa
   104161 aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt
   104221 ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact
   104281 ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct
   104341 tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta
   104401 gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc
   104461 atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg
   104521 atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata
   104581 gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt
   104641 ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag
   104701 aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc
   104761 aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg
   104821 tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag
   104881 ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca
   104941 aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt
   105001 caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg
   105061 ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg
   105121 cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg
   105181 cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc
   105241 ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac
   105301 taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt
   105361 cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc
   105421 agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc
   105481 tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt
   105541 tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca
   105601 gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct
   105661 aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat
   105721 aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt
   105781 tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat
   105841 aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa
   105901 cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa
   105961 ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc
   106021 ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc
   106081 tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg
   106141 aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc
   106201 tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt
   106261 gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt
   106321 caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc
   106381 tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc
   106441 cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg
   106501 atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg
   106561 catataagta cggtaattca atattgcagc ggctacttct aattctccca gcaattccca
   106621 cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat
   106681 aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact
   106741 cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt
   106801 cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga
   106861 aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata
   106921 gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag
   106981 aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc
   107041 aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc
   107101 taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc
   107161 aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa
   107221 cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc
   107281 caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt
   107341 gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc
   107401 ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa
   107461 taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc
   107521 cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa
   107581 aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt
   107641 atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt attctccagt
   107701 gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt
   107761 ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat
   107821 acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt
   107881 tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga
   107941 taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat
   108001 cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc
   108061 ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc
   108121 caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga
   108181 ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt
   108241 tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt
   108301 tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt
   108361 gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa
   108421 acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat
   108481 atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta
   108541 ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta
   108601 gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata atcatacgta
   108661 tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact
   108721 atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt
   108781 ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg
   108841 tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca
   108901 tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa
   108961 acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct
   109021 actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact
   109081 tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg
   109141 tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa
   109201 acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga
   109261 agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact
   109321 cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag
   109381 ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt
   109441 aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg
   109501 aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt
   109561 tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca
   109621 actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc
   109681 atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat
   109741 cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac
   109801 atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac
   109861 gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt atcactgatt
   109921 ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca
   109981 taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt
   110041 tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt
   110101 actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg
   110161 atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt
   110221 gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt
   110281 ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa
   110341 caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa
   110401 gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca
   110461 ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag
   110521 taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat
   110581 aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta
   110641 ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat
   110701 atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg
   110761 acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca
   110821 ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa
   110881 caaaaggaat cagcataatt gattttagac taataacttg catcggtact tttacttttt
   110941 tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca
   111001 taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag
   111061 caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac
   111121 caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa
   111181 aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa
   111241 ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact
   111301 cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca
   111361 ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct
   111421 cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa
   111481 attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct
   111541 ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg
   111601 atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga
   111661 gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc
   111721 atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct
   111781 tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca
   111841 gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga
   111901 ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct
   111961 ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt
   112021 tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt
   112081 ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc aaacgacgaa
   112141 ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa tgtttcataa
   112201 attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac agaatgaaca
   112261 agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt
   112321 ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt
   112381 tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt
   112441 ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt aaaaaagcaa
   112501 aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa ctgtagatac
   112561 tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat aacaggaaaa
   112621 tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt tctatcttgt
   112681 agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc gttatttatt
   112741 ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat cactttcgtt
   112801 ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta agcgatttca
   112861 tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc tacacttatc
   112921 ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata gatatttact
   112981 cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt gatcaattct
   113041 tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc aacacccata
   113101 tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt ttcaatcgaa
   113161 gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga aaatgctttt
   113221 tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga attattacta
   113281 cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg gatagctaaa
   113341 gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg attaagcaat
   113401 tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc aatgaaattg
   113461 atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc ccaataatag
   113521 ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat gcgcacgcca
   113581 tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac atctttcatt
   113641 gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg aatctttaga
   113701 gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc cggagagtct
   113761 gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt tccaataagc
   113821 gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg gattatttct
   113881 ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg ttctgataat
   113941 tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat ttgccgttcg
   114001 ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac aattattatc
   114061 tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata ttcttgatta
   114121 tttcaaatca ataaaaaagc ctgcaatatt tatatcacag gcttttttct ctattaaata
   114181 tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg
   114241 caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat
   114301 tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg
   114361 gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg
   114421 tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt
   114481 tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa
   114541 catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat
   114601 atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa
   114661 agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa
   114721 ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc
   114781 ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa
   114841 aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc
   114901 ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag
   114961 gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc
   115021 agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa
   115081 acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc
   115141 cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa
   115201 tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa
   115261 gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt
   115321 ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca
   115381 gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat
   115441 gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat
   115501 agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa
   115561 gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa
   115621 gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt
   115681 ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca
   115741 gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct
   115801 acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta
   115861 ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg
   115921 tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg
   115981 cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga
   116041 cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca
   116101 gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa
   116161 cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca
   116221 cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat
   116281 tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa
   116341 agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa
   116401 tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg
   116461 tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg
   116521 agacccacat tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca
   116581 tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga
   116641 ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca
   116701 attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga
   116761 gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga
   116821 gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata
   116881 ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg
   116941 atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga
   117001 tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt
   117061 gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta
   117121 ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc
   117181 gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca
   117241 atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc
   117301 gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga cttacccgct
   117361 ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct
   117421 aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt
   117481 ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa
   117541 agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa
   117601 caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt
   117661 tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt
   117721 tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga taactatagc
   117781 aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc
   117841 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
   117901 cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
   117961 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
   118021 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
   118081 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
   118141 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac
   118201 aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt
   118261 atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa
   118321 aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc
   118381 agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta
   118441 acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc
   118501 attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat
   118561 atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat
   118621 ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct attcagtcaa
   118681 taaaaatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt atttttttgt
   118741 gcttaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat tatgtgtatt
   118801 aactattttg agtttggctg ttcaagcaat tgttggagca attgctccta cgattacttt
   118861 tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag cacatcaata
   118921 tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg ttccgcatta
   118981 tgcagtgtac aacggcttta aacaagatat tttctgtatt gaaccaggag tcccgattta
   119041 caatgagttc actcctggct atgagaaaaa cccgttacca gatatgtccg ataaagccaa
   119101 attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata ttgtggcaca
   119161 gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac gatcagatgg
   119221 tagtgccgta aatatcgcag ccattgaggc aaaaataaat caagccattg acgattatca
   119281 gaagaaacca accttccata atagtacggc taaaacagta ttagggcaat cgactactgt
   119341 gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa atgccgcaaa
   119401 cattgattat cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca attcaaaaga
   119461 aagtggtgtg ctgacgctta agaaatctgc tggtaccgga acacctgtcg cctataaaaa
   119521 ggctggccaa cagactttaa tggccggggc gattgataaa cctaatacct acacaatcaa
   119581 aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag aatcgggtga
   119641 tgttgtacca ggaacggttt tccatttaga ctttggaaaa actttacctg caaaagacgt
   119701 gactactgac aaagaaggca ttgctacatt ggatgagatt cctcatggta caaaagtaac
   119761 cattaccgaa aaatcagtac cggcgcctta tacgattgat accaccccca tgactaccac
   119821 cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa aagggcaaat
   119881 cattctggat aaaagcggag ttgaaacagg gagtgatctt tggaatgaca attattcctt
   119941 agctggtaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg ttcaagaaat
   120001 gaccacggat gaaaacggtc atgcggaaac accaaaagag attgctaatg cgttggaatt
   120061 gggaacctat tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata cgttcaaacc
   120121 tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc cctgttacca gtaacgtaaa
   120181 agggcaaaat caagaagtca ctggcgaaac cactttgacg aaagaagaca aagatactgg
   120241 ggataaagca caaggtaagg cagtctttga gggtgctgaa tacaccttgt ttactgctaa
   120301 ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca gagttagcga agggaacgaa
   120361 aggttctgat gaaacggtca ctttgacttt agacgaaaag aaccaagttg ccgttaaaca
   120421 tttagctatc aacgagtatt attgccaaga aacaaaagcg cccgaaggat atagccttga
   120481 tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa atgccgtgat
   120541 tacccgaaat gttacggcaa aagaacaagt gattcgcttt ggctttgatt tctttaaatt
   120601 tgctggatca gctgcgggca ctaccgaaac tggcttcaat gatctgacct ttaaagtgtc
   120661 gccattggaa gggaccaatg aaatcacagg tgccgaagat gaagcgacca cagcttataa
   120721 cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg gggattattt
   120781 acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag aaatccgttc
   120841 tacttttaag gaaaacaaag aagactttgt gaagagtgag tatgtcttta ccattaccga
   120901 acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc tgacgaacaa
   120961 agcattttct gttagtttga accgtttaat gctctatgat ttgcctgagg aagaagatag
   121021 tttaacttct cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc tggattctac
   121081 cgagctagtt gataaattga gttacaatct acatgaaatc aaagaagact ggtatgtggt
   121141 agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg aaaaagccaa
   121201 accagtggtg attgcagaaa caagcgcaac gttggcgaac aaagagaaaa caggaacttg
   121261 gaaaattcag cataaattaa ccgccgaaca agttttaaat aaaactattg tcttattcaa
   121321 ctatgtgtat gaaaataagg aagcttttga agcaggcgat aagccagtag cgaaggatgt
   121381 tagcttgaat aaccaagccc aaactgtcag ttgtacagtg gaacaccatg tttctatcca
   121441 aacaaaggcc cacctagaaa acggttctca gacctttact catggtgatg tggtggatat
   121501 gtttgatgat gtgtcgatta ctcatgatgt actggatggc tcaaaagaag ctttcgaaac
   121561 aattctgtat gctttattac cggatggtac aaacaaagaa atttggaaat ctggcaagat
   121621 tgattatgaa gtaaatgata aagaattcac caaaactgtg cttgcgaaaa aagtagatac
   121681 cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa atcaattacg ataaagatgg
   121741 gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct taacaccaaa
   121801 agaagttcca accatactaa gtacgccgaa acaaccggaa acaccaactg ttccaagtgg
   121861 ctctcaagaa tctagtccca cagtgaagac cttccctcaa accggggaga aaaattccaa
   121921 tgttttactg ttcattggtt ttactttgat ttttgcgacg gcgggctatt atttctggaa
   121981 tcgccggaac taaggtgatg agatgataca agtaaatcct aagaaccaac gtgcgcgacc
   122041 gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa ctaacaatga aaagaggaaa
   122101 agaagatgga attgaaattt gtcgtgccag atatggctga aacctttgga aagttaagtt
   122161 atgccggtga aggagaaatt ataacagaag gatatggacg gaatactacg gtgattggtc
   122221 gcagttatca tttgtattcc agtaagcaac gggccgatga tattgaggtg gtagtagctg
   122281 cggaagccgg tgaaaaggat tttgatcaag accaaccgct aaaagccgtg aatccccatt
   122341 tggttgccaa aggctatgag attgagaatc gtgggtttac tgattacgtg ttgtatgttg
   122401 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
   122461 attcaggttt aacatttggg aagttgcgtt tttccgcatt acgccgagaa gtacggaagc
   122521 aaaatgagga tggaacaatt ggtaatgagg taaaagaacg aacctataat ttaaaatcct
   122581 ctgcacaagg gcgaatgatt caagttagta tcccagctaa tgtaccctta cgcgagtttg
   122641 cttatgatgc agaggtagag ttggtcaatc caatcgtgga taccgttgcc aattatgttt
   122701 ttcgagaagg aactaccgtt aattggttca ttaaggcgga tgatttagtc ttgaagcggc
   122761 agccgaatca aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg aagattatgg
   122821 aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggc gagctgactg
   122881 aggaacaact cgttaaagca ttaacctctg atgaaaaaat gtttctgatt atacatcaag
   122941 gtttacttgg agaaaatagc gacagtgaag aagactctga tgtactagac tggttaggag
   123001 aagaagagtc tttctttata gttgtcgaag taaatgaaaa cttatgtcgg caggcagaat
   123061 ccattttgga agaaattggt gtggagatgc cggatgctat tgaaggcttt ttaaatcaat
   123121 tggttgaaac gaaacaactc ccctcctcgt agcagtaaat gactagcata actttttgaa
   123181 attgcctaat gaaatcctat tgggcttttt cgattggagg tggaaaccat caaaatgtac
   123241 aaaggacatc gcatacgcgc aggagatcag cacttggttt atcactttgt tcttggctgg
   123301 ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt tagacaattt
   123361 gatatttcta agctatcact ttctactatt gaaatcgttt ggtctataaa agatttagta
   123421 tgtctgcttg gaagtctcgc tttttcagga gctatgatat tgctttatat tcactttttt
   123481 cttgatcatt ggcgaagctt gtggcatcga caaaagctag ctcggatgat tctagagaac
   123541 cattggtatg aagtgaaaca aactcaaagt gaaggctttt tcaaagattt gaatagtagc
   123601 cgaaccagag agactatcag ttacttcccc aaaatctgtt atcgaatgaa agatggatta
   123661 ctgtctattc gcgttcaaat ttcacttggg aaataccaag atcagctttt aaaattggaa
   123721 aagaagttag aaagcggact gtattgtgag ttagtagaaa aggaactcaa agactcctat
   123781 gtggagtata ctttactgta tgatatgatc gccaatcgaa ttgggataga cgaagtagtg
   123841 gcagaaaatg ggactctgcg attgatgaaa aatcaagtat gggcttatga ttctttaccc
   123901 catatgttaa tcgctggtgg cacgggtgga gggaagacct atttcctcct caccattatc
   123961 gaagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa tgcagattta
   124021 gctgatttag gtacggtgat gcctcatgtt tattcacaaa aggaagaaat ttctgcttgt
   124081 gtggaagatt tctatgaacg tatgatggct cgtagcaagg caatgaaaga aatgtctaac
   124141 tacaaaacag gagagaatta tgcctatctt ggacttccac ccaacttttt aatctttgat
   124201 gaatacgtgg cttatatgga gatgttaacg acgaaagaaa gtgcggtgat tttgaataag
   124261 ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt ggcttgccaa
   124321 aggccggatg cgaaatattt aggagacgga atccgcgatc aattcaactt tcgcgtggct
   124381 ttgggtcgta tgagtgaact cggatattcc atgatgtttg gggaggtcga aaaaaacttc
   124441 tttatgaagc acatcaaagg tcgaggttat gtggatacag gtggcagtgt gattagtgag
   124501 ttttatacgc cacttgtacc aaaagggtat gatttcttag attcaattag taaattagaa
   124561 aagatgaatg attgagtgat cttcaatact gactttatac ttcaagtaac gtataatact
   124621 tttatatagt aggaagaaag gggataatta atatggaaag taatgattct tatttagagt
   124681 atgcaaaagc aaataaggat aagtttattt cttatattat caaagggaaa tcccccgaag
   124741 ttggacgaga cgcagttttt atggcaggga gtcctggggc tggaaaatca gaggttgcca
   124801 ttggattagc tgagaattat gataaccatg tgattattga tgcggattac tttcgcaccc
   124861 agtttcctga ttataatgga aaaaactcta gtctctttca aaaagcatct tcttggttgg
   124921 tagagcaggc atttagatat gtcttagatc atggctattc ttttattctg gatgcaactt
   124981 ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat tttcgagtga
   125041 cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa gaacgtgaaa
   125101 ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa cgcttttttc aaatcaagag
   125161 agaacattgc aaaagtaaag agtaaacatc cagatgtatt gcttcatatc ataataaaag
   125221 attatacgaa taatatttcc gaagttcatt atgctacgga taatctagaa ttatttttac
   125281 cactagggta tagtaggaaa gatttggagg ttcaattaca tgactgagaa agaacaagta
   125341 acgaaaattg taaagaaata caataagagc attgcggatt tatcggaaaa tgctactgca
   125401 aaagagttca aaacggtgat taaatatgta gccgatcagg ccaatgaaaa acaacggaaa
   125461 ttagttggat taaataaaaa atagtaggac aaatta
//
LOCUS       DACDEA010000002        78403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDEA010000002 DACDEA010000000
VERSION     DACDEA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.002"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            221..592
                     /locus_tag="IV136_000115"
     CDS             221..592
                     /locus_tag="IV136_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8464136.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(866..1558)
                     /locus_tag="IV136_000116"
     CDS             complement(866..1558)
                     /locus_tag="IV136_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8464137.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1561..2346)
                     /locus_tag="IV136_000117"
     CDS             complement(1561..2346)
                     /locus_tag="IV136_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8464138.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2583..3983
                     /gene="lacG"
                     /locus_tag="IV136_000118"
     CDS             2583..3983
                     /gene="lacG"
                     /locus_tag="IV136_000118"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8464139.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4008..4325
                     /locus_tag="IV136_000119"
     CDS             4008..4325
                     /locus_tag="IV136_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8464140.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4338..6011
                     /locus_tag="IV136_000120"
     CDS             4338..6011
                     /locus_tag="IV136_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464141.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6025..7656
                     /locus_tag="IV136_000121"
     CDS             6025..7656
                     /locus_tag="IV136_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8464142.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8147..9712)
                     /gene="guaA"
                     /locus_tag="IV136_000122"
     CDS             complement(8147..9712)
                     /gene="guaA"
                     /locus_tag="IV136_000122"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8464143.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9771..10694)
                     /locus_tag="IV136_000123"
     CDS             complement(9771..10694)
                     /locus_tag="IV136_000123"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8464144.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10890..11495
                     /locus_tag="IV136_000124"
     CDS             10890..11495
                     /locus_tag="IV136_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8464145.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11803..12819
                     /locus_tag="IV136_000125"
     CDS             11803..12819
                     /locus_tag="IV136_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8464146.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12960..14261
                     /locus_tag="IV136_000126"
     CDS             12960..14261
                     /locus_tag="IV136_000126"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8464147.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14307..14969
                     /gene="deoC"
                     /locus_tag="IV136_000127"
     CDS             14307..14969
                     /gene="deoC"
                     /locus_tag="IV136_000127"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8464148.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14990..15388
                     /locus_tag="IV136_000128"
     CDS             14990..15388
                     /locus_tag="IV136_000128"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8464149.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15454..16536
                     /locus_tag="IV136_000129"
     CDS             15454..16536
                     /locus_tag="IV136_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464150.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16748..18310
                     /locus_tag="IV136_000130"
     CDS             16748..18310
                     /locus_tag="IV136_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464151.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18303..19436
                     /locus_tag="IV136_000131"
     CDS             18303..19436
                     /locus_tag="IV136_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464152.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19436..20392
                     /locus_tag="IV136_000132"
     CDS             19436..20392
                     /locus_tag="IV136_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464153.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20491..21657
                     /gene="deoB"
                     /locus_tag="IV136_000133"
     CDS             20491..21657
                     /gene="deoB"
                     /locus_tag="IV136_000133"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8464154.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21681..22499
                     /locus_tag="IV136_000134"
     CDS             21681..22499
                     /locus_tag="IV136_000134"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8464155.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22516..23223
                     /gene="deoD"
                     /locus_tag="IV136_000135"
     CDS             22516..23223
                     /gene="deoD"
                     /locus_tag="IV136_000135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8464156.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23521..24207)
                     /gene="gpmA"
                     /locus_tag="IV136_000136"
     CDS             complement(23521..24207)
                     /gene="gpmA"
                     /locus_tag="IV136_000136"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8464157.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24379..24960)
                     /locus_tag="IV136_000137"
     CDS             complement(24379..24960)
                     /locus_tag="IV136_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8464158.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25093..25776)
                     /gene="rpiA"
                     /locus_tag="IV136_000138"
     CDS             complement(25093..25776)
                     /gene="rpiA"
                     /locus_tag="IV136_000138"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8464159.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26095..26508
                     /gene="rpsL"
                     /locus_tag="IV136_000139"
     CDS             26095..26508
                     /gene="rpsL"
                     /locus_tag="IV136_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8464160.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26592..27062
                     /gene="rpsG"
                     /locus_tag="IV136_000140"
     CDS             26592..27062
                     /gene="rpsG"
                     /locus_tag="IV136_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8464161.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27156..29243
                     /gene="fusA"
                     /locus_tag="IV136_000141"
     CDS             27156..29243
                     /gene="fusA"
                     /locus_tag="IV136_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8464162.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29415..30602
                     /gene="tuf"
                     /locus_tag="IV136_000142"
     CDS             29415..30602
                     /gene="tuf"
                     /locus_tag="IV136_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8464163.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31009..31317
                     /gene="rpsJ"
                     /locus_tag="IV136_000143"
     CDS             31009..31317
                     /gene="rpsJ"
                     /locus_tag="IV136_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8464164.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31348..31977
                     /gene="rplC"
                     /locus_tag="IV136_000144"
     CDS             31348..31977
                     /gene="rplC"
                     /locus_tag="IV136_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8464165.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32005..32628
                     /gene="rplD"
                     /locus_tag="IV136_000145"
     CDS             32005..32628
                     /gene="rplD"
                     /locus_tag="IV136_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8464166.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32628..32918
                     /gene="rplW"
                     /locus_tag="IV136_000146"
     CDS             32628..32918
                     /gene="rplW"
                     /locus_tag="IV136_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8464167.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32957..33790
                     /gene="rplB"
                     /locus_tag="IV136_000147"
     CDS             32957..33790
                     /gene="rplB"
                     /locus_tag="IV136_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8464168.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33833..34111
                     /gene="rpsS"
                     /locus_tag="IV136_000148"
     CDS             33833..34111
                     /gene="rpsS"
                     /locus_tag="IV136_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8464169.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34134..34481
                     /gene="rplV"
                     /locus_tag="IV136_000149"
     CDS             34134..34481
                     /gene="rplV"
                     /locus_tag="IV136_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8464170.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34495..35151
                     /gene="rpsC"
                     /locus_tag="IV136_000150"
     CDS             34495..35151
                     /gene="rpsC"
                     /locus_tag="IV136_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8464171.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35154..35588
                     /gene="rplP"
                     /locus_tag="IV136_000151"
     CDS             35154..35588
                     /gene="rplP"
                     /locus_tag="IV136_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8464172.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35578..35766
                     /gene="rpmC"
                     /locus_tag="IV136_000152"
     CDS             35578..35766
                     /gene="rpmC"
                     /locus_tag="IV136_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8464173.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35791..36057
                     /gene="rpsQ"
                     /locus_tag="IV136_000153"
     CDS             35791..36057
                     /gene="rpsQ"
                     /locus_tag="IV136_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8464174.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36115..36483
                     /gene="rplN"
                     /locus_tag="IV136_000154"
     CDS             36115..36483
                     /gene="rplN"
                     /locus_tag="IV136_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8464175.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36520..36828
                     /gene="rplX"
                     /locus_tag="IV136_000155"
     CDS             36520..36828
                     /gene="rplX"
                     /locus_tag="IV136_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8464176.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36856..37395
                     /gene="rplE"
                     /locus_tag="IV136_000156"
     CDS             36856..37395
                     /gene="rplE"
                     /locus_tag="IV136_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8464177.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37414..37599
                     /locus_tag="IV136_000157"
     CDS             37414..37599
                     /locus_tag="IV136_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8464178.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37635..38033
                     /gene="rpsH"
                     /locus_tag="IV136_000158"
     CDS             37635..38033
                     /gene="rpsH"
                     /locus_tag="IV136_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8464179.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38065..38601
                     /gene="rplF"
                     /locus_tag="IV136_000159"
     CDS             38065..38601
                     /gene="rplF"
                     /locus_tag="IV136_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8464180.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38762..39118
                     /gene="rplR"
                     /locus_tag="IV136_000160"
     CDS             38762..39118
                     /gene="rplR"
                     /locus_tag="IV136_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8464181.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39139..39639
                     /gene="rpsE"
                     /locus_tag="IV136_000161"
     CDS             39139..39639
                     /gene="rpsE"
                     /locus_tag="IV136_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8464182.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39654..39833
                     /gene="rpmD"
                     /locus_tag="IV136_000162"
     CDS             39654..39833
                     /gene="rpmD"
                     /locus_tag="IV136_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8464183.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39877..40317
                     /gene="rplO"
                     /locus_tag="IV136_000163"
     CDS             39877..40317
                     /gene="rplO"
                     /locus_tag="IV136_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8464184.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40317..41612
                     /gene="secY"
                     /locus_tag="IV136_000164"
     CDS             40317..41612
                     /gene="secY"
                     /locus_tag="IV136_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8464185.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41674..42321
                     /locus_tag="IV136_000165"
     CDS             41674..42321
                     /locus_tag="IV136_000165"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8464186.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42512..42730
                     /gene="infA"
                     /locus_tag="IV136_000166"
     CDS             42512..42730
                     /gene="infA"
                     /locus_tag="IV136_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8464187.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42763..42879
                     /gene="rpmJ"
                     /locus_tag="IV136_000167"
     CDS             42763..42879
                     /gene="rpmJ"
                     /locus_tag="IV136_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8464188.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42897..43262
                     /gene="rpsM"
                     /locus_tag="IV136_000168"
     CDS             42897..43262
                     /gene="rpsM"
                     /locus_tag="IV136_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8464189.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43290..43679
                     /gene="rpsK"
                     /locus_tag="IV136_000169"
     CDS             43290..43679
                     /gene="rpsK"
                     /locus_tag="IV136_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8464190.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43759..44697
                     /locus_tag="IV136_000170"
     CDS             43759..44697
                     /locus_tag="IV136_000170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8464191.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44725..45105
                     /gene="rplQ"
                     /locus_tag="IV136_000171"
     CDS             44725..45105
                     /gene="rplQ"
                     /locus_tag="IV136_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8464192.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45377..46216
                     /locus_tag="IV136_000172"
     CDS             45377..46216
                     /locus_tag="IV136_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8464193.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46192..47061
                     /locus_tag="IV136_000173"
     CDS             46192..47061
                     /locus_tag="IV136_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8464194.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47051..47848
                     /locus_tag="IV136_000174"
     CDS             47051..47848
                     /locus_tag="IV136_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8464195.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48051..48797
                     /gene="truA"
                     /locus_tag="IV136_000175"
     CDS             48051..48797
                     /gene="truA"
                     /locus_tag="IV136_000175"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8464196.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49252..51921)
                     /locus_tag="IV136_000176"
     CDS             complement(49252..51921)
                     /locus_tag="IV136_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8464197.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52159..52347)
                     /locus_tag="IV136_000177"
     CDS             complement(52159..52347)
                     /locus_tag="IV136_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8464198.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52617..52979
                     /locus_tag="IV136_000178"
     CDS             52617..52979
                     /locus_tag="IV136_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8464199.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53031..54713
                     /locus_tag="IV136_000179"
     CDS             53031..54713
                     /locus_tag="IV136_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8464200.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54830..56866
                     /gene="recG"
                     /locus_tag="IV136_000180"
     CDS             54830..56866
                     /gene="recG"
                     /locus_tag="IV136_000180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8464201.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56915..57916
                     /gene="plsX"
                     /locus_tag="IV136_000181"
     CDS             56915..57916
                     /gene="plsX"
                     /locus_tag="IV136_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8464202.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58038..58280
                     /gene="acpP"
                     /locus_tag="IV136_000182"
     CDS             58038..58280
                     /gene="acpP"
                     /locus_tag="IV136_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8464203.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58548..59552
                     /locus_tag="IV136_000183"
     CDS             58548..59552
                     /locus_tag="IV136_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464204.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59553..60497
                     /locus_tag="IV136_000184"
     CDS             59553..60497
                     /locus_tag="IV136_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464205.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60497..61459
                     /locus_tag="IV136_000185"
     CDS             60497..61459
                     /locus_tag="IV136_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464206.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61486..62400
                     /locus_tag="IV136_000186"
     CDS             61486..62400
                     /locus_tag="IV136_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464207.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62426..64207
                     /locus_tag="IV136_000187"
     CDS             62426..64207
                     /locus_tag="IV136_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464208.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64555..65241
                     /locus_tag="IV136_000188"
     CDS             64555..65241
                     /locus_tag="IV136_000188"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8464209.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65261..68842
                     /gene="smc"
                     /locus_tag="IV136_000189"
     CDS             65261..68842
                     /gene="smc"
                     /locus_tag="IV136_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8464210.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68851..69660
                     /locus_tag="IV136_000190"
     CDS             68851..69660
                     /locus_tag="IV136_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8464211.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69671..70669
                     /gene="ftsY"
                     /locus_tag="IV136_000191"
     CDS             69671..70669
                     /gene="ftsY"
                     /locus_tag="IV136_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8464212.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70856..73123
                     /gene="gshAB"
                     /locus_tag="IV136_000192"
     CDS             70856..73123
                     /gene="gshAB"
                     /locus_tag="IV136_000192"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8464213.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73212..73664)
                     /locus_tag="IV136_000193"
     CDS             complement(73212..73664)
                     /locus_tag="IV136_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8464214.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73814..74764
                     /locus_tag="IV136_000194"
     CDS             73814..74764
                     /locus_tag="IV136_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8464215.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74757..75716
                     /locus_tag="IV136_000195"
     CDS             74757..75716
                     /locus_tag="IV136_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8464216.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75713..76468
                     /locus_tag="IV136_000196"
     CDS             75713..76468
                     /locus_tag="IV136_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464217.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76491..77444
                     /locus_tag="IV136_000197"
     CDS             76491..77444
                     /locus_tag="IV136_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464218.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78088..78384)
                     /locus_tag="IV136_000198"
     CDS             complement(78088..78384)
                     /locus_tag="IV136_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8464219.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT        25233 a        14192 c        17043 g        21935 t
ORIGIN      
        1 tttctaaagt ttgtttctta attagcttat tgattctata atatttttga tttatatagg
       61 aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa tagatttaat
      121 aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga
      181 attttttcaa gattactttg cggagtatgc cgataggata atgagagatt acgtaacaga
      241 taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg ataaggcgaa
      301 agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata tgagtggtga
      361 aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta tcaaaacttt
      421 tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat taattaatat
      481 ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt tgtttacaga
      541 taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt aaccattacg
      601 cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag attggtttga
      661 gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa cgcgtcatat
      721 ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga tatagcagtg
      781 tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat ttcagttgct
      841 tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac aggtgctctg
      901 tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc tgttcaaata
      961 ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc gcacgtttgg
     1021 cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga gctaagctca
     1081 gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa gagccgtcgt
     1141 catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca aaataaaaag
     1201 caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct
     1261 ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt tgcaagcaaa
     1321 gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag gcggtcaaat
     1381 gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata ttttttaaaa
     1441 tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc caagcagctt
     1501 catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc cctttcatag
     1561 ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga catattgtgc
     1621 atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat cgaaatcatg
     1681 caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa cgagtagata
     1741 tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt
     1801 agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa actttagtct
     1861 tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc
     1921 gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa ctggataatt
     1981 atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt cgattgttgt
     2041 acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg tggcagcaat
     2101 ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt cttgatctaa
     2161 agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg atttttctaa
     2221 ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga tgatttcatt
     2281 aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc tttctttttt
     2341 caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac aacagtcaat
     2401 ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa tagattttat
     2461 tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat gttttggatg
     2521 cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga ggagagatgt
     2581 acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc tatcaggttg
     2641 aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac
     2701 aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca aaggacattg
     2761 aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg agccgcattt
     2821 ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg
     2881 aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt gataccccta
     2941 aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca tttgtttcct
     3001 atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa
     3061 tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga atcaaatatg
     3121 attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc cgtgtagtga
     3181 actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca ttagagacga
     3241 aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac gatgcattgt
     3301 cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa accttgactg
     3361 ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa gaagatttcg
     3421 ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac taccaatgcc
     3481 attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg ggtgaaaaag
     3541 ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa ggaattcctc
     3601 gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg ctgcgaatta
     3661 aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg gggtataaag
     3721 atcagtttga agatggcatt attatggatc agccgagaat cgactatttg agagtctatt
     3781 tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat tttttgtggt
     3841 cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc ttgttttatg
     3901 ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac aagttggtca
     3961 gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg aataaagagg
     4021 aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc agtacattat
     4081 tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg gcgttgaaag
     4141 aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta gcggaagaag
     4201 catctgggaa agagatggag atgggattca tttttatcca tgggcaggat catttaatga
     4261 ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga cagaatcaag
     4321 gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt
     4381 ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt ttaacagcga
     4441 tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct gagatttttg
     4501 gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat tattcgatgg
     4561 gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat tcgatgaatc
     4621 gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa
     4681 gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa tatatgggga
     4741 caaagggact actggcttcc tttgtcgctg catttttaac agttaattta tataagtttt
     4801 gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa
     4861 ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta attgatttag
     4921 gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg ctgcagccgc
     4981 tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg atggcgctgt
     5041 tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct
     5101 atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct tctaaagtac
     5161 tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca acattggttg
     5221 ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt
     5281 cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca cccatgattt
     5341 tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt tgcctgttca
     5401 agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct tgggcaactc
     5461 cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt gttctagcag
     5521 cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca tacgatgctg
     5581 tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg
     5641 ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat gcaaataaag
     5701 aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgcgatgtta gctaatgctt
     5761 taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt
     5821 ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa gtcaattctt
     5881 actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt gcgacaaaag
     5941 gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt attctgaacc
     6001 aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa agctgtttta
     6061 tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc atctgccagc
     6121 gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa
     6181 tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa tggtgtcaac
     6241 tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag ttatggagca
     6301 gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa tctgggactt
     6361 aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa acagaatctt
     6421 cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta cgactataca
     6481 aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt tcagatcggc
     6541 aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga aggcggggga
     6601 gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg agacactcaa
     6661 cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg agcatttact
     6721 tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat
     6781 tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc gatcagccaa
     6841 cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac agcgaatgcc
     6901 gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata tgaaccaaca
     6961 ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc agtaccgaat
     7021 aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa tgtgagttgg
     7081 gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa cgaatgggac
     7141 aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa
     7201 gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg accaacgacc
     7261 agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg
     7321 gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca agcatacgag
     7381 gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt gagtgcctat
     7441 gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta tggtggagcg
     7501 gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat caaagacatt
     7561 ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg aaatgcttgg
     7621 acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt cgattcaaga
     7681 tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac ctgcacctcg
     7741 cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg agtaaagcac
     7801 cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg taagaagcaa
     7861 tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa gggtaacctt
     7921 agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt tctcggaaaa
     7981 cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa cttatattat
     8041 agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt tagctattga
     8101 agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat tcccactcca
     8161 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     8221 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     8281 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     8341 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     8401 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     8461 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     8521 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     8581 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     8641 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     8701 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     8761 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     8821 atacaccttt caacttgtct aagaagcgat ctcttgcatt gactttgatg atgttcaagc
     8881 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     8941 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     9001 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     9061 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     9121 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     9181 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     9241 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     9301 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     9361 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     9421 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     9481 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     9541 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     9601 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     9661 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     9721 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     9781 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     9841 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     9901 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     9961 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
    10021 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
    10081 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
    10141 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
    10201 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
    10261 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
    10321 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
    10381 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
    10441 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
    10501 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
    10561 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
    10621 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
    10681 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
    10741 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
    10801 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
    10861 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
    10921 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
    10981 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
    11041 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
    11101 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
    11161 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
    11221 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
    11281 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
    11341 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
    11401 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
    11461 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
    11521 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
    11581 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
    11641 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
    11701 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
    11761 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
    11821 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
    11881 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
    11941 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
    12001 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
    12061 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
    12121 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
    12181 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
    12241 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
    12301 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
    12361 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
    12421 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
    12481 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
    12541 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
    12601 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
    12661 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
    12721 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
    12781 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
    12841 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
    12901 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
    12961 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
    13021 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
    13081 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
    13141 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
    13201 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
    13261 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
    13321 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
    13381 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
    13441 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
    13501 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
    13561 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
    13621 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
    13681 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
    13741 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    13801 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    13861 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    13921 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    13981 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    14041 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    14101 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    14161 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    14221 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    14281 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    14341 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    14401 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    14461 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    14521 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    14581 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    14641 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    14701 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    14761 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    14821 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    14881 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    14941 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    15001 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    15061 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    15121 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    15181 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    15241 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    15301 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    15361 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    15421 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    15481 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    15541 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    15601 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    15661 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    15721 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    15781 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    15841 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    15901 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    15961 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    16021 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    16081 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    16141 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    16201 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    16261 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    16321 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    16381 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    16441 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    16501 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    16561 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    16621 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    16681 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    16741 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    16801 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    16861 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    16921 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    16981 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    17041 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    17101 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    17161 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    17221 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    17281 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    17341 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    17401 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    17461 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    17521 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    17581 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    17641 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    17701 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    17761 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    17821 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    17881 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    17941 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    18001 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    18061 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    18121 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    18181 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    18241 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    18301 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    18361 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    18421 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    18481 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    18541 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    18601 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    18661 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    18721 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    18781 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    18841 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    18901 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    18961 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    19021 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    19081 gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta
    19141 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    19201 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    19261 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    19321 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    19381 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    19441 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    19501 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    19561 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    19621 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    19681 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    19741 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    19801 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    19861 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    19921 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    19981 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    20041 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    20101 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    20161 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    20221 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    20281 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    20341 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    20401 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    20461 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    20521 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    20581 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    20641 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    20701 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    20761 ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc
    20821 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    20881 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    20941 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    21001 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    21061 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    21121 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    21181 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    21241 gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat
    21301 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    21361 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    21421 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    21481 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    21541 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    21601 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    21661 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    21721 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    21781 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    21841 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    21901 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    21961 tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg
    22021 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    22081 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    22141 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    22201 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    22261 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    22321 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    22381 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    22441 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    22501 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    22561 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    22621 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    22681 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    22741 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    22801 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    22861 atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg
    22921 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    22981 gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt
    23041 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    23101 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    23161 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    23221 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    23281 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    23341 ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
    23401 cagcttttca tgtttgatga atattgtgac ttgtgaccga gaagttggca ggagaaaacc
    23461 gtttatacga aaaaagggac tgtgacaaat cgtgtcacag tccctttttt atagtgtttt
    23521 ttataggtag tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg
    23581 acctgttgga atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc
    23641 tagggcgcgt aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc
    23701 tggagcaatt tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc
    23761 tccaccagga atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc
    23821 ttcgtcagtt tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac
    23881 ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata
    23941 gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc
    24001 taagatcaag ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa
    24061 ttcgattcca gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg
    24121 tgctagattt acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc
    24181 gtgacgagaa aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt
    24241 ctgaaatttt cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat
    24301 tagataagat tctatgagaa aacaagtaga gaatataaaa aaacgaacag tgcccacgca
    24361 actgttcgtt tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat
    24421 atatttataa catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc
    24481 ttttttttca taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct
    24541 tccgccgatt tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat
    24601 caatgcgcac tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc
    24661 gcgcgtatcg attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg
    24721 cgtgagttct gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag
    24781 tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt
    24841 ttgttccact tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc
    24901 aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat
    24961 ggtgattcct ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa
    25021 gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca
    25081 agcagttttt ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc
    25141 gacaactact ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc
    25201 atctaaatag gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat
    25261 gtaatgacca ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg
    25321 gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac
    25381 tgggagtggg aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt
    25441 ttcttttgag tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat
    25501 cccttgaaaa tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac
    25561 ttcatcaata ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga
    25621 agtagtaact cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac
    25681 catataatag gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt
    25741 cgcggcttca atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt
    25801 tgagcatcgt gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt
    25861 tttattccaa taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt
    25921 cgtgatattc taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt
    25981 ttatcaaagc atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag
    26041 aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct
    26101 acaattaacc aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct
    26161 gctttgaaca aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa
    26221 aaacgtgggg tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta
    26281 cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg
    26341 attggtcata acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac
    26401 ttaccagggg tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac
    26461 cgtaaacaaa gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta
    26521 aacaaatgaa acttgcttct actatataga agtaattact atgaacaata tttcggaagg
    26581 aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta
    26641 tttataactc aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg
    26701 ggattgcagc taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg
    26761 atccattaga agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag
    26821 cacgtcgtgt tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg
    26881 ttactctagg tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg
    26941 aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa
    27001 aacgtgaaga cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt
    27061 aagattctct ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta
    27121 gcaactagat agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa
    27181 acactcgtaa tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac
    27241 gtatcttgta ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac
    27301 aaatggactg gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta
    27361 cagcacaatg gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca
    27421 cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac
    27481 aatcaggtgt tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac
    27541 ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat
    27601 caactttaca tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg
    27661 aagacgactt cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg
    27721 atcttggaac agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag
    27781 aatggcgcga aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat
    27841 acttagaagg tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag
    27901 ttaacgtaga attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat
    27961 tactattgga cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag
    28021 ggattgaccc taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct
    28081 cagctttagc atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg
    28141 tgtactctgg tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg
    28201 aacgtgttgg tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt
    28261 acgctggaga tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat
    28321 gtgatgaaaa gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag
    28381 tcgctattga acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac
    28441 tatctgaaga agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca
    28501 tcgctggtat gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca
    28561 aagttgaagc taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca
    28621 ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat
    28681 ggattgaatt tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg
    28741 gtggggttgt tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg
    28801 aaaatggtgt cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt
    28861 cataccatga tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac
    28921 gtgcagcagc taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg
    28981 ttatccctga agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg
    29041 ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg
    29101 ctgaaatgtt tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta
    29161 caatgacatt tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga
    29221 aaaacggcgg aaaagctgaa taatttcttg aaaccggata gtcctgcaaa gagattatcc
    29281 aaagagacta tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt
    29341 ggtgtacctt gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag
    29401 gaggacattt taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg
    29461 gtactatcgg acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac
    29521 tatctaagaa aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag
    29581 aacgcgaacg cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc
    29641 actacgctca cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg
    29701 ctgctcaaat ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa
    29761 ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga
    29821 acaaagtaga catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg
    29881 acctattaac agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt
    29941 tgaaagctct agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag
    30001 ttgacgaata catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg
    30061 aagacgtgtt ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac
    30121 aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa
    30181 cagttactgg tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca
    30241 ttggtgcttt actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta
    30301 aaccaggtac aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag
    30361 aagaaggtgg acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta
    30421 caactgacgt aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg
    30481 acaacgtaac aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat
    30541 tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt
    30601 aattcactta agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag
    30661 gtcttaggat ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga
    30721 gtattataaa tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat
    30781 gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat
    30841 gacaaaggtg cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct
    30901 aagaaacgag aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt
    30961 ttcgtggagc tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa
    31021 aaaattcgta tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa
    31081 atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact
    31141 gaacgcagtc tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa
    31201 ttcgaaatgc gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt
    31261 gatgctttga tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac
    31321 taaaacaaaa ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga
    31381 atgacacaaa tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact
    31441 ccaaacgtag tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt
    31501 ggttaccaag atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa
    31561 gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat
    31621 gaagtaggaa aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca
    31681 ggtactacga aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga
    31741 ccaatggctc acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct
    31801 aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc
    31861 caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat
    31921 gttcctggag ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg
    31981 taggaagaaa ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc
    32041 caaaacggtg aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt
    32101 gtttacgatg caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa
    32161 aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt
    32221 gcacgtcaag gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca
    32281 acaccacgtt cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct
    32341 gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac
    32401 gcaccaaaaa caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta
    32461 ttggttgttc tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac
    32521 gtttctgtag taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg
    32581 ttagcaacac aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag
    32641 acgtaatcaa acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat
    32701 acacttttga agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg
    32761 cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta
    32821 tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag
    32881 attcaaaaga aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa
    32941 attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa
    33001 catgacaggt tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca
    33061 accattgaaa aaccgtgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg
    33121 tggcggacac aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt
    33181 tgcaactgtt caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca
    33241 ctatgaagat ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa
    33301 acttgtttca ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat
    33361 tccagttggt actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat
    33421 ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg
    33481 cttgaactct ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt
    33541 aggtaacgaa caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg
    33601 taaacgccca actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg
    33661 tgaaggtaaa gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct
    33721 tggttacaaa actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa
    33781 aaataaataa ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg
    33841 tagtttaaag aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc
    33901 aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag
    33961 ttttgttgga tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca
    34021 agaagacatg gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca
    34081 tgctgctgac gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag
    34141 aacaaatcac atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc
    34201 gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat
    34261 tcacaccaaa caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg
    34321 cagaaaataa ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg
    34381 aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca
    34441 aacgtacaag tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt
    34501 caaaaagtac atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg
    34561 tatgctgaaa aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt
    34621 tcaactaaac ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc
    34681 gtaaacattt caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa
    34741 gtcgaaagcc taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc
    34801 gtggaaatca aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa
    34861 ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt
    34921 gctggcgctc aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct
    34981 cgttcagaag gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat
    35041 tacgcatggg aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat
    35101 cgtggagaaa ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag
    35161 tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag
    35221 gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca
    35281 caaaccgcca aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga
    35341 aagtatggat taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta
    35401 tgggttctgg taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca
    35461 tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc
    35521 acaaattacc aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg
    35581 aaggttaaag aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta
    35641 aaagaagaat tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca
    35701 cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct
    35761 aagtaattat ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt
    35821 tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca
    35881 aagaaaaacc accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat
    35941 gatgaaaaca acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta
    36001 tcagctacaa aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa
    36061 cacgagattt tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc
    36121 caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc
    36181 aaagtccttg gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg
    36241 gttaaacaag caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc
    36301 gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat
    36361 gctgcagtga ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt
    36421 gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta
    36481 taatcttgag acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat
    36541 aaagttaaag ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc
    36601 ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct
    36661 tctcaagctg ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac
    36721 gtgatggtgg ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc
    36781 aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa
    36841 ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca
    36901 ttgatggaaa aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc
    36961 atcaacatgg gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa
    37021 gaattaactt tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct
    37081 ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga
    37141 atgtacgaat tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat
    37201 ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa
    37261 ttgatcttcc cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt
    37321 attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg
    37381 ccattccaaa aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa
    37441 aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt
    37501 ccacattcag tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat
    37561 aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag
    37621 gaggtaaaag atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta
    37681 acgcaaacat ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca
    37741 tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca
    37801 aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa
    37861 acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa
    37921 aagtattgaa cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata
    37981 aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta
    38041 gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct
    38101 gaaggagtaa cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa
    38161 ttgactcgta ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc
    38221 actcgtccaa acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc
    38281 aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt
    38341 gggtaccgtg cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca
    38401 gtagaaatca caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt
    38461 gttaaaggtg cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt
    38521 cctccagaac cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa
    38581 gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa
    38641 aggcatttgg ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta
    38701 tttttcgaaa cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat
    38761 tgtgattaca aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa
    38821 caatatctct ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat
    38881 ctacgctcaa attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga
    38941 taaagaaatt tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc
    39001 tgaacgtgca gctgaaaaag gcattaaagt agtagttttt gaccgtggtg gataccttta
    39061 ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa
    39121 gaaggaggaa taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc
    39181 gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct
    39241 gctttagttg ttgtcggtga caaaaacgga cacgtaggat ttggtacagg taaagctcaa
    39301 gaagttcctg aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta
    39361 cctatggttg gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc
    39421 ctaatgaaac ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta
    39481 ttagaattag ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc
    39541 aacgttgttc gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca
    39601 gaacttcgcg gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg
    39661 aattaaaagt tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag
    39721 ttaaagcgtt aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa
    39781 ttaaaggcat gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa
    39841 aaatcataac tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct
    39901 gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga
    39961 aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta
    40021 ggatttgaag ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac
    40081 gtgaaccgta aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga
    40141 gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga
    40201 atcaaagtat tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc
    40261 tctaaagcag ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt
    40321 ttagactatt aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg
    40381 tatttgtgtt gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg
    40441 cttcattgca aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta
    40501 gtgcgatgca aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta
    40561 tcgtcattca gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag
    40621 gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat
    40681 ttatccaatc tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga
    40741 ataatcctaa cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt
    40801 ttgttacttg gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga
    40861 tcatttttgc aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt
    40921 actttataaa tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac
    40981 tagtagcggt cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga
    41041 tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga
    41101 aagtaaatgc agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa
    41161 atgctgtttt acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga
    41221 tggagatctt taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg
    41281 ccttcacttt cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc
    41341 aaaagcaagg aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct
    41401 caagattact gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc
    41461 ttccaatcat tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa
    41521 gcttactgat cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc
    41581 tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca
    41641 cacgttctca cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca
    41701 ggcgcaggta aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc
    41761 tcaacaggag atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca
    41821 aagtcttata tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa
    41881 gagcgactag ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg
    41941 ctagatcaag caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct
    42001 gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc
    42061 tgcagcaatt gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca
    42121 tgtgatcgtt gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt
    42181 aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa
    42241 caaggtttgc taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta
    42301 caaaaaatca ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc
    42361 ttgtcaagtt caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag
    42421 atgatccatt ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa
    42481 aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga
    42541 aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca
    42601 ccaagttctt gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg
    42661 tgacaaagta acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg
    42721 ctttaaataa ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca
    42781 gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt
    42841 tgcccagcaa atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg
    42901 ctcgtattgc aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata
    42961 tctatggtat tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag
    43021 atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata
    43081 aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg
    43141 aaatcggttc ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca
    43201 cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat
    43261 aattcttaag tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc
    43321 gtgtgaagaa gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta
    43381 tcatcatgat cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag
    43441 gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta
    43501 caaaagcagc aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt
    43561 ctggacgtga agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc
    43621 gtgacgtgac tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat
    43681 gagtgccgct cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg
    43741 aaaggggtaa agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa
    43801 gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta
    43861 gggaattccc tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt
    43921 caaatcgatg gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact
    43981 caaatcatct tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc
    44041 cttgaaatcg atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt
    44101 gatgtagaaa ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc
    44161 catgctcgct taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa
    44221 gaagatatgc caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg
    44281 aactatcaag ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg
    44341 gagatttgga cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc
    44401 atgactgaac atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc
    44461 atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta
    44521 gacttgtctg ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa
    44581 cttacaaata aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta
    44641 gaagaagtta aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac
    44701 cttttacgaa ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa
    44761 cgtaaagcga tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact
    44821 actgaagctc gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa
    44881 cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc
    44941 gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat
    45001 attgcacctc gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca
    45061 agacgcggtg atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac
    45121 atcactttat agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct
    45181 agctcagcgc tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt
    45241 ttttttatac cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt
    45301 taagcgagaa ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga
    45361 tgaagaagga gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca
    45421 gccagatgat cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg
    45481 gatcgctatt attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg
    45541 attgcttctg ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa
    45601 tatttggacg atccgacaga tggttggaat ggtttttcaa aatccagaca atcagtttgt
    45661 tggttcgaca gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga
    45721 agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc
    45781 acgcgagcct gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt
    45841 tgccttacga cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg
    45901 tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat
    45961 atctatcact catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca
    46021 aggcgaactt gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat
    46081 cgacttaggt ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg
    46141 ggttgatgtg cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc
    46201 cgttttgaac aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta
    46261 tttgacatca atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga
    46321 agcgggaaat caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca
    46381 gtccatattg gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc
    46441 cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta
    46501 gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc
    46561 ctagcaaaag aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca
    46621 tttgaacttt caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg
    46681 ccggaagtac ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa
    46741 atgatggaga tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg
    46801 catttaatgg atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga
    46861 atcgttaata gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa
    46921 caactaggcg tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac
    46981 tttgctacct tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga
    47041 ggaaatgccc atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat
    47101 ccatcgattg gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt
    47161 tttagcaaat aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt
    47221 cttgtcaaaa gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat
    47281 cctcttcact gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg
    47341 gggtgttttc catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt
    47401 cgtgttgatc atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc
    47461 ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat
    47521 ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa
    47581 gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa
    47641 aatgaaagca gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga
    47701 agatcttgcg actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata
    47761 tcgtgtattg cattggcata atagagacac gatggttatc attgcttttg tattattaac
    47821 tgtgctactg atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt
    47881 gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg
    47941 gcagactaag gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat
    48001 agaaaaatca ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt
    48061 acaaagcaat cattgcgtac gacggaacaa attttaatgg attccaaaaa cagccaaatg
    48121 ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa
    48181 tcacggtctt tggttcggga aggacagatg ccggagtaca tgcgatcggg caagtaatcc
    48241 attttgatta tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt
    48301 caccagagga tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat
    48361 atttggtcaa agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt
    48421 ttcgacgcca ttatgcaagt tattttcctt acccacttga tttggaaaaa attcagcggg
    48481 cgcttcctga tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga
    48541 tagaggataa aatacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg
    48601 aattgttgtt tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag
    48661 gaacgctttt gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg
    48721 cgaaaaaaga tcgtaatgca gcaggaccta cagcacaccc tgaaggactt tatttgtatg
    48781 aagtgaaata tgaataagag gttatgatag aagtggtcag cttcaagaaa taagaaggta
    48841 tttacgaaaa ttacttctca aaattttgca aaatttcggc ttattttcga agaagctact
    48901 tctggaacac cgtttaagga agaggtcgtg aaatcagcgt tttgacctga gtattagaga
    48961 aacaaacggg aaaatagctc ctcatatttt gccgtttgtt gagctaatac cgaaggtcag
    49021 ctgattgaac accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaaa
    49081 gagcaatttc aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg
    49141 ttgataagtg aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg
    49201 ttgttttgat tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc tttattttct
    49261 acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa tcgtgccata
    49321 aaaggggcga ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac
    49381 aaaaaggatt gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt
    49441 acctgctgga acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt
    49501 cccatcaatt ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc
    49561 ttcagcggta tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt
    49621 ttctttgatc tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg
    49681 ctccatgaaa cttatttctt tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc
    49741 ttgaggataa tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga
    49801 tgtaccattg acagtgatcg tcgcactaga actttctaat tgcgcaaaat tggttgggaa
    49861 caaacttaag taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg
    49921 ctggttatct gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt
    49981 caactgattc ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta gaccagacaa
    50041 tgtattgatc aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt
    50101 aacggacaac ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata
    50161 gtcgccgctt tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga
    50221 aatgttatat ttgattcctg taaacccatc catcagcaaa gtattattgt tataacggat
    50281 attcaaattt gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg
    50341 atttcttata gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc
    50401 agatactgga tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt
    50461 tacaagagct ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca
    50521 atcatcaaga atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat
    50581 caataataac aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac
    50641 agcaaaagca taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata
    50701 ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa
    50761 aatgattcct aacaacagcc cacgatcgtc ttgttctatc ttttcccatc cttttcctgc
    50821 aagcaggagc actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc
    50881 atgcatacca tgccagaaca aattcaatgg aacaagataa aaacttgcga taagtatggc
    50941 gaaaagactg ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa
    51001 cagacagagg actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt
    51061 cgtgtcataa acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt
    51121 tttcaacgta gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg
    51181 caatacaatg atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat
    51241 cgttttttta tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa
    51301 tacgccaatc ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa
    51361 taaaagtttc ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag
    51421 ataaaaaaat gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa
    51481 tgcgtaactg acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag
    51541 aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc
    51601 tggcatcatc tggtttggaa aaaagaacac cagtggcgtg aagatcccgc ctagatagta
    51661 agaaattaaa gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg
    51721 ctttccaaga agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc
    51781 acttgccaaa atacttctgc tgcttccagg atagattccg atacttagat aaacaagtgc
    51841 taaaatcaaa aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa
    51901 tgactttaaa ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt
    51961 ttatcatatc cttgtttttc tctgcttaaa aagagtctag ttttcacaaa aaaataaaat
    52021 ttagaataaa taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag
    52081 gaatcaaaag tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac
    52141 ggtccaagta tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga
    52201 cacccaaact tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt
    52261 aacagtacgt tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg
    52321 acctgtaaag taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa
    52381 tagaataatt cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca
    52441 ttttcataga tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct
    52501 gaaattttgc aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat
    52561 gataaaatat gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg
    52621 ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag
    52681 ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa
    52741 ttaaagataa tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc
    52801 gccaagaaga taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa
    52861 tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag
    52921 gcgtaactgc caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg
    52981 caggagactg gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa
    53041 cagaaataag tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag
    53101 taaatgcaga atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa
    53161 caaatatgaa tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa
    53221 aagtaggaga attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt
    53281 ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct
    53341 cattaaatgc ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag
    53401 cagtaatgaa accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg
    53461 gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag
    53521 gagcaaagcg tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg
    53581 tcgtagacag tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga
    53641 atggagaatt ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg
    53701 tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg
    53761 gttactgtac agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg
    53821 attacgaaga attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg
    53881 atgacgagat catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg
    53941 gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga
    54001 cgattttgga acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc
    54061 cgtatggtat cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag
    54121 gcgctaatta tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta
    54181 aagcgattca agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct
    54241 tcatggcagc tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa
    54301 gaacgatttc ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag
    54361 aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg
    54421 cgatcagaga tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga
    54481 tcgatggaaa aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag
    54541 aacaaatgat caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag
    54601 ttgaacaagc aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg
    54661 agatccacca aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt
    54721 gtcagaatat ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt
    54781 atgatcgctg accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt
    54841 agatgatcca atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag
    54901 cttgggcatc caaacgattg aagacttact gacttattat ccttttcgct acgaagatat
    54961 tcaggaaaga aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt
    55021 agtctcgcct cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat
    55081 gatgcaagaa catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa
    55141 agtcatttta tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt
    55201 gaacgggatg aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt
    55261 caacaaatca attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata
    55321 cggcacattg attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga
    55381 ccgagcttct gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc
    55441 caaacgacgt gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa
    55501 aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa
    55561 acaatttact caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga
    55621 aatctgtcgg gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg
    55681 tagcggaaaa acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca
    55741 aggtgcgttg atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca
    55801 gctgtttgat cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga
    55861 acgacgtgag ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca
    55921 tgcattgatc caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca
    55981 gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt
    56041 cttatttatg acagcgactc cgattcctag aacattagct attacagcat atggtgagat
    56101 ggatgtatct atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt
    56161 aagaacacct caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca
    56221 tcaaatgtat gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc
    56281 agtggaaatc tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt
    56341 acatggtaaa atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca
    56401 gatgcagata ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac
    56461 cgtcatgctg atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg
    56521 acgtgttgga cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga
    56581 actaggtgtg gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga
    56641 aaaagatctg gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc
    56701 tcaatttgcg attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga
    56761 ggctgcagcc ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc
    56821 gcaaagcctc caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata
    56881 ctttatactg atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg
    56941 cggagatcac gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc
    57001 agaagtagaa ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga
    57061 aaaaaacatc acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa
    57121 agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg
    57181 tgaagcagat gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt
    57241 catcgtagga cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt
    57301 agggaaagaa ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc
    57361 agaacatttg ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat
    57421 cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt
    57481 gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt
    57541 agaagctcgt gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg
    57601 caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc
    57661 tgctattcta aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt
    57721 acgttctttg aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt
    57781 gaaagcacca gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat
    57841 ccgccaaatc cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga
    57901 aaaaagcgaa gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg
    57961 acattcctga aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat
    58021 tcttggaggt gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt
    58081 caaatcattt tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt
    58141 taaaagctga ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta
    58201 cggaaatttc agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata
    58261 tcagcagcca tcttaaataa tttcaaataa aagcaaagaa ggataaaaag ctgcatgcag
    58321 ctttttatcc ttcttttttt aatctttttg acacgtaaaa gtcatttttc tgataattct
    58381 ggcgataaac agaaattatg ttgcaaagta aagcggtttt ctatataatt attaataatt
    58441 cattacacaa agataagaat aacctttgtt ataaaacgat ttattctgtg taatgtcctt
    58501 ttttattaag aaaaacttcc atttaggaag cagagagggg tattcgattg tccgataacc
    58561 aattattaga agttcagaac ctacatacag gattccgact caaagatgac tattacgatg
    58621 cagtagatga cgtttcattt actttagaca aaaacgagat tttagcaatc gttggggaat
    58681 ctgggtgcgg gaaaagtacc ttggctacta cgattatggg attacttgat cctaataaca
    58741 caaaaattac aggtgagatt ttatataacg atctgaattt aatcgacctg aacgaaacgc
    58801 tctataataa aatccgcgga aatgatatcg gaatgatttt ccaagatccg ttgtctgcat
    58861 taaatcctct catgagaatc gaagaccaga tcaaagaagg actttcctat cacacaaaga
    58921 tgaatgcaga acagcgtcaa gctcgtgcac tagaattgct agaacaagta gggattccta
    58981 atcctgcgag agttggacgc caatatcccc atgaactatc aggcggtatg agacaacgtg
    59041 tcatcatcgc aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat gaaccgacga
    59101 cagccctaga tgtaaccatc caagcacaga ttcttgattt actgaaagac cttcaggaag
    59161 aaacaaaaac agggatcatc ttgatcactc atgacttagg agtcgtagca gaaatggctg
    59221 atcgagtggc agtgatgtat ggcggacagt tcgttgaagt agcaggtgta aaagaactgt
    59281 ttgatcatcc taagcatcct tatacacgct ctttactgaa ctctatacca caagaaggaa
    59341 atcatgatgc agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc
    59401 gtaaaggttg tcggtttgca ccacgtattc catggatacc agcaagcgat catgaagaag
    59461 agcctatcct tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc tataagcatt
    59521 tccactttag agacgaaaaa ggagaggtgt agatgacaga attaatcaca atcaaagacc
    59581 tgaaagttca ttaccctatt cgcagtggat tcttcaatcg agtaacggat catgtatatg
    59641 ctgtagatgg tgtggatttt attatcgaaa aaggaaagac atatggttta gtgggagagt
    59701 ctggctctgg aaaatctact acgggaaaag ccgttgtggg attagaaaag gtaacagccg
    59761 gacaaatcat gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt
    59821 ataacaagga tgtgcagatg atcttccaag attcaatgtc cagtttgaat cctaaaaagc
    59881 gagtgttgga tatcattgct gaacccatcc gaaattttga acggttaagc gatcaagaag
    59941 aaaagaaaaa ggtaaaagga ctgctagata tcgttgggat gccggaagat gcgctatata
    60001 aatatcctca tgaattttca ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg
    60061 ctaccaatcc aaaactgatc gtagccgatg aacctgtatc tgctctggat ctatctgttc
    60121 aagcgcaagt attgaatttc atgaaacgaa tccagcaaga atacggcctc agctatttat
    60181 tcatttcaca tgatttaggt gtagtaaaac acatgtgcga caatatcgca attatgtaca
    60241 aaggtcgatt tgtagaaatc ggtacaagag aagacattta taatgacccg cgacatatct
    60301 atacgaaacg tctgctttca gctattccgc aaatcgatgt agaaaatagg gaaagccaca
    60361 aagaaaatcg acgaagagta gaacaagagt atatccaaaa ccaaaaagaa tactatgatg
    60421 cgactggtcg agtctacgat ttgcgtacga tcacaactac acacaaagtg gcgttgaaag
    60481 atggaggtgc aagctaatgt ggaaaacgat tctgcgacgt gttttattga tgatcccgca
    60541 agttattatt ttaagtgttc tgatcttctt attagcccaa gcgatgccgg gggatccatt
    60601 taccggtttg atcaatccaa atcaggatcc ggcagtgatc gaacagatgc gacaagcagc
    60661 tggcttgaat gacccatggt atgtccagta cttccgttgg atcggaaatg ctttgcaagg
    60721 cgattttggt caaagtttct tatacaaacg ctctgttgct gacttgatcg gcgaacggat
    60781 catcaacact ttatatctat cgatcttgac agttgtcctt acttatttga ttgctgtgcc
    60841 tctggggatg cttgctggcc ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta
    60901 caacttcttt agttttgctg ttcctttatt tatcttcggc ttgatcatgc tatttgtttt
    60961 tggttatcgt ttaggctggt tcccaacaag tggatcacaa acactaggtt tgcaaggtgg
    61021 atttatcact caatggatag accgtttgaa atatattctg ttaccatcaa ttacccaagc
    61081 atttttggcg actgccgtca caatccaata tctgcgaagt gaagtaatcg attcgaaatc
    61141 gctcgatttt gtcagaacag cccgttccaa aggggtaccg acgaataaag tattcagtcg
    61201 acatattttc cgaaatgcag cgttgccgat ggcttctcaa atggggtatg aaatcacttc
    61261 gttgattgct ggttctgtag ttatcgaaaa gatttttggt tatccgggag tcggaaaact
    61321 atttattgat tccatcggcc aacgggacta taccgtgatc acatccttag tattgatttt
    61381 aggaatcgcg acgctagtgg ggacattact ttcagatatc atcatgagta tcgttgaccc
    61441 gcgtattcgg attcaataaa accaatcaga aaggagaatg gatcgatgag tgaaaaaaac
    61501 aatacagcag ttcaagaaag tattcctcct atggggatac gaatgattgc tcgagaattt
    61561 aaaaaagata aaattgctat tttttcatta ggtttgctag tggttttatt attagtgatc
    61621 tttatcggtg cgatgctgac cgatcaagac aaagtgatgt atgttagtat cttcgacaaa
    61681 tacgctgcac caggaacagt ttcgacacaa ggacaaaagt tcattctggg agcagatgaa
    61741 ggcggacgtg atgtattagg acaattgatc ataggtgcta gaaactctgt tacgatcgga
    61801 tttgcgatca cgatccttac ttcgatcatc ggggttggat tagggatcat ctctggctac
    61861 tacggaggga tcattgacaa catcttgatg cggatcgttg attttatcat gattctaccg
    61921 atcatgctga tcattatcgt cttcgtatcg attatttcca gatacaatgt ctggtccttt
    61981 atattcatca tgagtgcatt ttattgggta gctaaagccc gattattccg aagcaagaca
    62041 ttatctgaag caagaagaga ttatgtcagt gcctcaaaaa caatgggaac caatgatttt
    62101 aaaatcatgt tccgcgaaat catgccgaat ctaagttcat taatcattac taatttgacg
    62161 atgaactttg cggcaaacat cggtatcgag acaactttga ctttcttagg tttcggcttg
    62221 ccggcaaatg tacctagttt aggtacattg atcggttatg caagtaacgg agaagtctta
    62281 gtgaatcggc aatggatctg gttacccgca tcaatcctta ttttagtttt gatgttgagt
    62341 atcaattatg ttggacaagc attcaagcgt tctgcagatg cgcgccaacg gttaggctag
    62401 tatagaagcg aagggggaaa taggaatgaa gaaaacgatt tttggatttg cagcgctgat
    62461 gtctgctgtc gttcttgcag catgcggggg cggaggtaca gatacctctg gaaacaactc
    62521 tggaggcaac ggcaataaca aagaggaatt aacttttcca attgcaacaa aaaataatga
    62581 tgatgcaatc gaagacggcg aattagaagc tgcggtcgca acggacagtc agtttaaagg
    62641 attgttccag tgggagtttt accaagattc atatgatgca gcttttatgg ctccttctca
    62701 tgaaccgcta tttgcaaacg atgacgattt cagaatcaca gacgacggag ctgcaacgct
    62761 tgatctagat gaagatacga aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc
    62821 agatggtgaa gatgtaacgg cggacgatgt gattttccct tacgaaatca ttgggaataa
    62881 agattacact ggtatccgtt atgacgacac tttccgtaac atcgtaggta tggaagaata
    62941 tcatgatgga agtgccgaca caatctccgg aatcaaaaaa atagacgaca aaacagttga
    63001 aatcgaatat aaagaagtaa acccaagcat gcttcaatcg ggtggtggta tctggtcata
    63061 tgcagcacca aaacatacgt tagaaggtgt agcaatcaaa gatatggaat caagcgatca
    63121 agtacgtaaa aatccagtga cttttggccc ttattacatg agtaatatcg ttgcaggaga
    63181 atcagtagaa tacttgccaa acgagcacta ctggaatggt acgcctaaat tgaaaaagat
    63241 cacgatgaaa tctgtaccga tggcaagtgc aactgaagca ttgaactcta aattatatga
    63301 tatgattttc caaatgccaa cagatactta cgatacctat aaagaaattg ctggctatac
    63361 aaacttaggc cgtgaacaaa cttcttatac ttatctagga ttcaagttag gtaaatggaa
    63421 tgcagataca aagagtgtgg aatacaatcc agattcaaaa atggctgata aatcattacg
    63481 tcaagcaatg ggctatgcat tagataataa tgcagtaggt gaacgtttct atgcaggttt
    63541 gagaagcaat gcgacttcat tgattcctcc agtattccag tcataccatg atgatgagat
    63601 gaaaggattc acacaagatc tagacaaagc gaaaaaacta ttagatgatg ctggctacaa
    63661 agatgtggat gacgatggtt taagagaaga caaagatgga aataaactaa caatcaactt
    63721 tgcttcgatg tccggtgggg aaacagcgca accattggca gattactata tccaacaatg
    63781 gaaagaaatt ggtttggatg tccaatacac aacaggtcgt ctgatcgaat tcaattcttt
    63841 ctatgataaa ttagaaaacg atgatcctga aattgatgtt taccaagctg cttggaatac
    63901 aggaactgat ccaaatccaa gtggactatg gggaccaaat gctgcgttca actacactcg
    63961 ttatgaatca gaagaaaata caaaattgat caacgatatc agttcaagca aagcatttga
    64021 tgaagagtac caaaaagaag catatcaaaa atggcaacaa tatgcatttg aagaagcatt
    64081 tgcgattcca acgttgttcc gtaatgaagt attgccagtc aacgaccgtg tcaaagactg
    64141 ggattggtct tatgatgtac caaacccatg ggcaaatgtt tctgttacag cagattcacg
    64201 ttcataatca ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt
    64261 ttctgtttat ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac
    64321 ggtgttcaaa gtccagtttc tcggtattaa gtcaaattta tgcaaaaata tggaaagcca
    64381 ttttcacaaa attttttctt catactcgaa actaaacgaa tttttcacaa cttctcgtca
    64441 atcactagga ttattaaagc tttacttacc ttttgaccga accatttcaa atttgacaaa
    64501 aaaccagtat aataaggaag tatcgaaaaa atatccataa aaggaatgtg gaaaatggac
    64561 aaccagctga caaaagaact aaaagaaaac tataacatcg tttttcacga tctaaatttg
    64621 cttgaacaag cttttaccca ttcatcctat gtgaatgagc atcgcaatct gcaattatcg
    64681 gataatgagc gacttgaatt tttaggagat gccgttttag aactgatggt ttctcagtat
    64741 ttattccgtt tatatcctca tttgccagaa ggaaaattga caaaaacacg agctgcgatc
    64801 gttcgcgaag acagcttgtc gaaatttgcc aaagaatgtc actttgatca atatgttttg
    64861 ttgggcaagg gagaagagaa ttcaggagga cgtacgcgac cagcattgct ttgcgaccta
    64921 ttcgaagctt ttttaggcgc gttatatctt gaccagggat tcgacacgac ccatgcattc
    64981 atcgaaaaag tgatttttcc taaagtcaaa gcaggtgctt tttcacatga gatggatcat
    65041 aaaacaaaac tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg
    65101 atcaatgaag aaggtccagc tcatgaacga gtattttgga tcgaagttta tgtagatgat
    65161 caattgattg gcactggaca agggaaatcg aaaaaattag cagaacaagc tgcggcggaa
    65221 aatgcattgg cagcactgta aaagtaagtg agggaaagct ttgtatttga aacgaattga
    65281 aatagccggt tttaaatcat ttgcagacaa gacagtcatt gattttgaaa atagcgtcac
    65341 agcagtggtt ggaccaaatg gcagcggaaa aagcaatatt acagaggcta tccgctgggt
    65401 attaggagaa caatctgcta aaagtctgcg aggcggaaaa atgccggata tcatctttgc
    65461 cggttccgat acccgtaagc agctgaatat tgcagaagtc acagtgattt tagacaatag
    65521 tgatcattat ctgccattgg agtacaatga gatcagtgta acccgccgtt atcgacggac
    65581 tggggaaagc gagtttttca tcaataaaca aagctgcaga ttaaaagata tccaagaatt
    65641 gttcttagat tctggattag gaaaagaatc gttctcaatc atttctcaag gaaaagtaga
    65701 agcgattttc agtagtaaac cggaagaccg tcgtgggatc tttgaagaag cagctggtgt
    65761 cttgaaatac aagcagcgga aaaagaaagc agaacagaag ctttttgaaa cagaagacaa
    65821 cctcagccgc gtccaagaca tcatccacga attggaagaa cagttaactc ctttggccgc
    65881 ccagagtgag gcagcaaagg agtttttacg gctaaaagaa acactgactc aaaccgatgt
    65941 ctctttgatg gttgctgaaa tcaagacagc aaaaaaagat tgggacaaca aacaagcaca
    66001 gcttgcgaaa ttcaatctag aattaggcaa actatctgag tcgatccaag aacaagaatc
    66061 cattttggcg aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca
    66121 gcaagtatta ttagatcttt cggaaaaact gaagcagaca gaaggacaaa aagatgtttt
    66181 gcaggagcgg acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga
    66241 agcacaaaaa aaggttaaac attttgaaaa gcttcaagaa agcttgatga aagcagcagc
    66301 agaaaaagaa acagagatcc aaaaagctga agcaaatctc ataaaaacac agcaagaatt
    66361 agaaaaatac caaaaatcaa cgaaagaatt attggcagaa ttaagagatc aatatgttga
    66421 tttgatgcaa gaacaagcag ctgtaggaaa cgaactgaag tatctagaac gtcaatatat
    66481 acaagaaaca gcaaaaagca agcagacgtt agccaagcaa tcggaagtag aagcatcagt
    66541 agaccgattg atcttacaaa aagaagaatt gacacaaaag caggcgcagc taaaatcatc
    66601 tttaactgaa acaaaagaga agttagaaat gatccaacaa aacgggaaaa agttccaaga
    66661 agcattagca aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc agcagctacg
    66721 tgctcgtaag aaaagccttc aagaaatcca agaaaactat tttggttttt accaaggggt
    66781 tcgattggtc ttgcagcata aacagcagct ttcgggtatt gtcggagcag tggctgaact
    66841 gatcgatgtt cctgcagatt tcacgttagc tatcgaagca gctttaggtg gggcagccca
    66901 gcatgtcatc gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca
    66961 aagaggagga agagcaacat tccttccact gacgacaatc aaaccaagac aattaccggc
    67021 tcatatattg acacaagcag cagctgtaga agggtttata gggattgcca gtgaacaagt
    67081 cagttatccc gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga tccttttagc
    67141 caaagactta actagtgcca atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt
    67201 ttccttagaa ggtgatgtga tgaatgccgg aggatccatg acaggtggag cgaataaacg
    67261 aggaaaccaa ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt
    67321 tgaagaagca gataaacaac tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga
    67381 aacagctcgt ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac aattacgttt
    67441 tgaagaacaa gaagcgacaa atcagctaca aaacatcata aatgaacttg aacgttttga
    67501 aaaagaaaag caaatctcaa cttttgagac acgtgaactg cagcagttta tcgaagatta
    67561 cgaaaagcaa acgaacgaat tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa
    67621 aatcgacgaa gaaatcaaat cgttaagtca agaaagcgac cagatggaag ccagaagagc
    67681 acaagtacaa agtcaaaaag cacaagaaca agcagatctt gctgtcttga aagagcagtt
    67741 taatcatctg cagatccagc tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg
    67801 tcaagaagcg atagaaaagc agttggcgac attgacagca gacttttctg atcatgaagt
    67861 cacagaagaa agtttggaaa aacaaattaa cgaattatct gctcagcgag aaacattaaa
    67921 agccgagttg gcaaaagcca aagagcagcg agatagaagg caaaaggaga tcgataagct
    67981 tgaagcagtc ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa
    68041 attagaagtc caaaaagacc gagctgaaat gattttagat aatcatttga cctatctgca
    68101 gtcagaatat cagctgacat ttgagaaagc ttcccaagac tatcaagaaa caaccgatat
    68161 cgaagacagt agaacaaagg tttcatcctt aaaagaacag attgaaaaac tgggaccagt
    68221 aaacttaaat tcaatcgaac aatacgaaca agtcagtgaa agacatacat tcttagcaac
    68281 acagcgagat gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga
    68341 tgatgaagtg cggacaagat tcaaagaagt atttgaagct atccgtcaag aattcaaaat
    68401 tgtctttcca aatatgtttg gcggcggtaa agctgaatta gtactgacgg aaccaactga
    68461 tttgctgaaa acaggtattg agattgaagt acaacctcct ggcaaaaaac tgcaaagtct
    68521 aagtttgctt tctggaggag aacgagcact tacagccatc gcgttgctct tttccattat
    68581 ccgtgtctgt cctgttccgt tttgtatctt agatgaggta gaagcagcgt tagatgaagc
    68641 caacgtgaaa cgttttggtc gttacttgag cgatttccaa gatgacacac aatttatcgt
    68701 agtcacacac cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag tgacgatgca
    68761 ggaatccggt gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg aagaggcgaa
    68821 aatcaacgaa acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat cgatttggac
    68881 ggtacattat taaatgaaca aaagctgatt tcagatgaga ataaacaagc attagcacaa
    68941 gcaaaacagc aaggggtcaa gatcgttcta tgtacaggac gccccttagc tgcgatggcg
    69001 cattatctgc aagaattagg actggtagat gaaggtgact acagcatcac tttcaacggc
    69061 ggtttggtac aaaaaaatga tactggtgaa atcatggaaa agaaagtgat ggaagtatca
    69121 gatatccaac gcttatacaa actggctcag caattggatc tgccactaga tgttttgtct
    69181 gacaatgtgg ttttacagct gcctagtgcc ccaaggaagc aatccttgta caatgtgttg
    69241 aataagctgc tgcaattcca accagcggat ataagcgata ttacagatga atttgtgctt
    69301 aataaggcag taatcgctta tgaccaagaa gaattagatc ctaaaatcaa agaaatacca
    69361 gcagaattcc atgaaatgta tgagatcatc aaaacaagaa gtatgctttt ggaatttatg
    69421 ccaaaaggtg ttacaaaagc ttatggtatt tctttattgg caaaagatct tggtttgaaa
    69481 caagaagaaa tcatggcgat cggtgatgag gaaaatgatt taccgatgat ccaatatgct
    69541 ggactaggcg tagcgatgga gaatgctgtg cctttcgtca aagaagcagc agatttcgtg
    69601 acagccagca atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt attagagtag
    69661 ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac
    69721 agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt
    69781 tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga
    69841 agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac
    69901 cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa
    69961 tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga
    70021 actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg
    70081 gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt
    70141 gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga
    70201 acgcgctggt gttgaagttg ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt
    70261 cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg
    70321 ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca
    70381 gcgggaatta ccagaagcac cccaagaaac acttcttgta ttagatgcaa ccactggtca
    70441 aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt
    70501 gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca
    70561 tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc
    70621 aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa
    70681 aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc
    70741 tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt
    70801 ctttttctta gactttttga ttataattag attcatatga attggaggtc acttaatgat
    70861 gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt
    70921 tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca
    70981 tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac
    71041 acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc
    71101 tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag
    71161 tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt
    71221 cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt
    71281 aagcggtatt catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca
    71341 tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagcgag
    71401 aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga
    71461 gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag
    71521 tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta
    71581 tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta
    71641 tacaccgctt cgtttccgag gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg
    71701 ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga
    71761 gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga
    71821 ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc
    71881 atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat
    71941 gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat
    72001 ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca
    72061 gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta
    72121 tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat
    72181 ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt
    72241 ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt
    72301 cccacttatc atggaaaaca aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg
    72361 ggtaccagga ggcgcagaat tttcatccat ggaagaagcg gtaaaagctt atccgagatt
    72421 tgccgagcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat
    72481 tttcaaggaa ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga
    72541 agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat
    72601 cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacagtat
    72661 ccgaacagtg aaagaacttg ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca
    72721 tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca
    72781 agggttgacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc
    72841 aaatatcagt acaggtggtg attcgatcga tatgactgat gaattttctg aagcttacaa
    72901 aaaaatcgcc gtttctgcgg tagaagcact aggagctaag attagtggta tcgatttgat
    72961 tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc
    73021 aaatttcaat ccagccatgc atatgcatgt ttatcctttt gcaggaaaag gcagaagatt
    73081 gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac
    73141 agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt
    73201 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    73261 tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    73321 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    73381 ttgactacag tgaaacggct attgtttttc gttgctagct ggatatattg gttttggaca
    73441 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    73501 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    73561 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    73621 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    73681 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    73741 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    73801 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    73861 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    73921 aagcttacaa atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg
    73981 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    74041 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    74101 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    74161 aacattgctt tttttatccc ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc
    74221 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    74281 attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg
    74341 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    74401 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatctgg gaatagatta
    74461 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    74521 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    74581 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    74641 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    74701 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataaggggac gacgatatgc
    74761 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    74821 tgtacttgac gatccataca tatggaaact ggcattttgc atgtgcattg aggagtaaga
    74881 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    74941 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    75001 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    75061 cagttttttt cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct
    75121 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    75181 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    75241 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    75301 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    75361 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    75421 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    75481 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    75541 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    75601 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    75661 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    75721 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    75781 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    75841 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    75901 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    75961 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    76021 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacat
    76081 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    76141 tgcgtatatt gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt
    76201 gaacaatcta gatatgaatt ttgcggttca aatcatgcag attttaaaaa atttagtaca
    76261 agaaatcggg aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc
    76321 agacgaaatt gttgcaatga aagatggaca gttatttacg caaggaacga cccaagaaat
    76381 gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga
    76441 aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    76501 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca
    76561 acaccagtca ggcagacaca aaaaaaagca gcacacaaac tgaaactaca ttgacgataa
    76621 cagatagtaa tggagaccaa atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg
    76681 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    76741 ctacaagcag ccttcctgag tatctttcat cctataaaaa agtagaatca gcaggtggaa
    76801 taaaagaacc agacttagaa aaaattaatc agcttcagcc agatctaatc atcatttctg
    76861 gccgacaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    76921 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    76981 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    77041 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    77101 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    77161 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    77221 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    77281 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    77341 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    77401 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    77461 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    77521 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    77581 ttatttattt gagaaatctt ttttcaacgt gaattcatta tctacatcat acgggttgat
    77641 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    77701 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    77761 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    77821 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaaacaa
    77881 atcgcagttg tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagc
    77941 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca
    78001 aatcgcagtt gtcacaatct cttctaaaca atgttcaatc agctgacctt cggtattagc
    78061 tcaaagcact gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt
    78121 gatgatctgc atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttagtaattg
    78181 atagaaataa ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt
    78241 cgtttttttt gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac
    78301 aaagagtgtt tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata
    78361 gatgtttttt atatattcct tcatagtgat tcctccaaag aag
//
LOCUS       DACDEA010000003        78013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDEA010000003 DACDEA010000000
VERSION     DACDEA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.003"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IV136_000199"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IV136_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV136_000200"
     CDS             complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV136_000200"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8464220.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3230..4519
                     /locus_tag="IV136_000201"
     CDS             3230..4519
                     /locus_tag="IV136_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8464221.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4558..5163)
                     /locus_tag="IV136_000202"
     CDS             complement(4558..5163)
                     /locus_tag="IV136_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8464222.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5368..5832)
                     /locus_tag="IV136_000203"
     CDS             complement(5368..5832)
                     /locus_tag="IV136_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8464223.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5854..6174)
                     /locus_tag="IV136_000204"
     CDS             complement(5854..6174)
                     /locus_tag="IV136_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8464224.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IV136_000205"
     CDS             complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IV136_000205"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8464225.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7597..7947
                     /locus_tag="IV136_000206"
     CDS             7597..7947
                     /locus_tag="IV136_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8464226.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8170..9117
                     /locus_tag="IV136_000207"
     CDS             8170..9117
                     /locus_tag="IV136_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8464227.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IV136_000208"
     CDS             complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IV136_000208"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8464228.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10741..11586
                     /locus_tag="IV136_000209"
     CDS             10741..11586
                     /locus_tag="IV136_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8464229.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11590..12030
                     /locus_tag="IV136_000210"
     CDS             11590..12030
                     /locus_tag="IV136_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8464230.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12080..12274
                     /locus_tag="IV136_000211"
     CDS             12080..12274
                     /locus_tag="IV136_000211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464231.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12410..13096
                     /locus_tag="IV136_000212"
     CDS             12410..13096
                     /locus_tag="IV136_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8464232.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13504..14193)
                     /locus_tag="IV136_000213"
     CDS             complement(13504..14193)
                     /locus_tag="IV136_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464233.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14168..16297)
                     /locus_tag="IV136_000214"
     CDS             complement(14168..16297)
                     /locus_tag="IV136_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8464234.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16345..17682)
                     /locus_tag="IV136_000215"
     CDS             complement(16345..17682)
                     /locus_tag="IV136_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8464235.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17934..18293)
                     /gene="rplT"
                     /locus_tag="IV136_000216"
     CDS             complement(17934..18293)
                     /gene="rplT"
                     /locus_tag="IV136_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8464236.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18346..18546)
                     /gene="rpmI"
                     /locus_tag="IV136_000217"
     CDS             complement(18346..18546)
                     /gene="rpmI"
                     /locus_tag="IV136_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8464237.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18618..19142)
                     /gene="infC"
                     /locus_tag="IV136_000218"
     CDS             complement(18618..19142)
                     /gene="infC"
                     /locus_tag="IV136_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8464238.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            19462..20094
                     /locus_tag="IV136_000219"
     CDS             19462..20094
                     /locus_tag="IV136_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8464239.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20096..21001)
                     /locus_tag="IV136_000220"
     CDS             complement(20096..21001)
                     /locus_tag="IV136_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8464240.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21086..21739)
                     /locus_tag="IV136_000221"
     CDS             complement(21086..21739)
                     /locus_tag="IV136_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8464241.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            22061..23005
                     /gene="mvk"
                     /locus_tag="IV136_000222"
     CDS             22061..23005
                     /gene="mvk"
                     /locus_tag="IV136_000222"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8464242.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22998..23975
                     /gene="mvaD"
                     /locus_tag="IV136_000223"
     CDS             22998..23975
                     /gene="mvaD"
                     /locus_tag="IV136_000223"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8464243.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23988..25073
                     /locus_tag="IV136_000224"
     CDS             23988..25073
                     /locus_tag="IV136_000224"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8464244.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25070..26134
                     /locus_tag="IV136_000225"
     CDS             25070..26134
                     /locus_tag="IV136_000225"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8464245.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26383..27186)
                     /locus_tag="IV136_000226"
     CDS             complement(26383..27186)
                     /locus_tag="IV136_000226"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8464246.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27206..28048)
                     /gene="kduI"
                     /locus_tag="IV136_000227"
     CDS             complement(27206..28048)
                     /gene="kduI"
                     /locus_tag="IV136_000227"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8464247.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28064..29071)
                     /locus_tag="IV136_000228"
     CDS             complement(28064..29071)
                     /locus_tag="IV136_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8464248.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29078..29722)
                     /locus_tag="IV136_000229"
     CDS             complement(29078..29722)
                     /locus_tag="IV136_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8464249.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29822..30580)
                     /locus_tag="IV136_000230"
     CDS             complement(29822..30580)
                     /locus_tag="IV136_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8464250.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30707..31858)
                     /gene="fucO"
                     /locus_tag="IV136_000231"
     CDS             complement(30707..31858)
                     /gene="fucO"
                     /locus_tag="IV136_000231"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8464251.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31940..32251)
                     /gene="rhaM"
                     /locus_tag="IV136_000232"
     CDS             complement(31940..32251)
                     /gene="rhaM"
                     /locus_tag="IV136_000232"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8464252.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32253..33098)
                     /gene="rhaD"
                     /locus_tag="IV136_000233"
     CDS             complement(32253..33098)
                     /gene="rhaD"
                     /locus_tag="IV136_000233"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8464253.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33112..34368)
                     /gene="rhaA"
                     /locus_tag="IV136_000234"
     CDS             complement(33112..34368)
                     /gene="rhaA"
                     /locus_tag="IV136_000234"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8464254.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34365..35825)
                     /gene="rhaB"
                     /locus_tag="IV136_000235"
     CDS             complement(34365..35825)
                     /gene="rhaB"
                     /locus_tag="IV136_000235"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8464255.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35978..36847
                     /locus_tag="IV136_000236"
     CDS             35978..36847
                     /locus_tag="IV136_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8464256.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36973..37872)
                     /locus_tag="IV136_000237"
     CDS             complement(36973..37872)
                     /locus_tag="IV136_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8464257.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38037..38861)
                     /locus_tag="IV136_000238"
     CDS             complement(38037..38861)
                     /locus_tag="IV136_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8464258.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38872..40167)
                     /locus_tag="IV136_000239"
     CDS             complement(38872..40167)
                     /locus_tag="IV136_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8464259.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40183..40662)
                     /locus_tag="IV136_000240"
     CDS             complement(40183..40662)
                     /locus_tag="IV136_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8464260.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40668..41696)
                     /locus_tag="IV136_000241"
     CDS             complement(40668..41696)
                     /locus_tag="IV136_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8464261.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41713..42819)
                     /locus_tag="IV136_000242"
     CDS             complement(41713..42819)
                     /locus_tag="IV136_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8464262.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43048..45645)
                     /gene="adhE"
                     /locus_tag="IV136_000243"
                     /gene_synonym="adhC"
     CDS             complement(43048..45645)
                     /gene="adhE"
                     /locus_tag="IV136_000243"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8464263.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45950..46330)
                     /gene="yajC"
                     /locus_tag="IV136_000244"
     CDS             complement(45950..46330)
                     /gene="yajC"
                     /locus_tag="IV136_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8464264.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46427..47572)
                     /gene="tgt"
                     /locus_tag="IV136_000245"
     CDS             complement(46427..47572)
                     /gene="tgt"
                     /locus_tag="IV136_000245"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8464265.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47790..48488
                     /locus_tag="IV136_000246"
     CDS             47790..48488
                     /locus_tag="IV136_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8464266.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48656..51295)
                     /locus_tag="IV136_000247"
     CDS             complement(48656..51295)
                     /locus_tag="IV136_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8464267.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51914..52945)
                     /gene="queA"
                     /locus_tag="IV136_000248"
     CDS             complement(51914..52945)
                     /gene="queA"
                     /locus_tag="IV136_000248"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8464268.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53138..54313
                     /locus_tag="IV136_000249"
     CDS             53138..54313
                     /locus_tag="IV136_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8464269.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            54317..54958
                     /locus_tag="IV136_000250"
     CDS             54317..54958
                     /locus_tag="IV136_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464270.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54955..55872
                     /locus_tag="IV136_000251"
     CDS             54955..55872
                     /locus_tag="IV136_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464271.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55869..56540
                     /locus_tag="IV136_000252"
     CDS             55869..56540
                     /locus_tag="IV136_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464272.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57081..57569)
                     /locus_tag="IV136_000253"
     CDS             complement(57081..57569)
                     /locus_tag="IV136_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8464273.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57770..58036)
                     /locus_tag="IV136_000254"
     CDS             complement(57770..58036)
                     /locus_tag="IV136_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8464274.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58036..58299)
                     /locus_tag="IV136_000255"
     CDS             complement(58036..58299)
                     /locus_tag="IV136_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8464275.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(58292..58564)
                     /locus_tag="IV136_000256"
     CDS             complement(58292..58564)
                     /locus_tag="IV136_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464276.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            58775..58960
                     /locus_tag="IV136_000257"
     CDS             58775..58960
                     /locus_tag="IV136_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8464277.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59102..60925)
                     /locus_tag="IV136_000258"
     CDS             complement(59102..60925)
                     /locus_tag="IV136_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8464278.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61523..64228)
                     /locus_tag="IV136_000259"
     CDS             complement(61523..64228)
                     /locus_tag="IV136_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8464279.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64534..65481)
                     /locus_tag="IV136_000260"
     CDS             complement(64534..65481)
                     /locus_tag="IV136_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8464280.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65671..65940
                     /gene="rpsN"
                     /locus_tag="IV136_000261"
     CDS             65671..65940
                     /gene="rpsN"
                     /locus_tag="IV136_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8464281.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66116..66478)
                     /locus_tag="IV136_000262"
     CDS             complement(66116..66478)
                     /locus_tag="IV136_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8464282.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66489..67610)
                     /locus_tag="IV136_000263"
     CDS             complement(66489..67610)
                     /locus_tag="IV136_000263"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8464283.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67626..67976)
                     /locus_tag="IV136_000264"
     CDS             complement(67626..67976)
                     /locus_tag="IV136_000264"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8464284.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68106..69617)
                     /locus_tag="IV136_000265"
     CDS             complement(68106..69617)
                     /locus_tag="IV136_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8464285.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69939..71291)
                     /locus_tag="IV136_000266"
     CDS             complement(69939..71291)
                     /locus_tag="IV136_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8464286.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71444..72520)
                     /locus_tag="IV136_000267"
     CDS             complement(71444..72520)
                     /locus_tag="IV136_000267"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8464287.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72716..74461
                     /locus_tag="IV136_000268"
     CDS             72716..74461
                     /locus_tag="IV136_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8464288.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74741..75178)
                     /locus_tag="IV136_000269"
     CDS             complement(74741..75178)
                     /locus_tag="IV136_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8464289.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75162..76535)
                     /locus_tag="IV136_000270"
     CDS             complement(75162..76535)
                     /locus_tag="IV136_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464290.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76857..77345)
                     /locus_tag="IV136_000271"
     CDS             complement(76857..77345)
                     /locus_tag="IV136_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464291.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77367..77969)
                     /locus_tag="IV136_000272"
     CDS             complement(77367..77969)
                     /locus_tag="IV136_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8464292.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22479 a        16610 c        14233 g        24691 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg
     2401 atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc
     2461 gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa
     2521 ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc
     2581 atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac
     2641 ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa
     2701 ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta
     2761 tttttctttt aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc
     2821 ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt
     2881 tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa
     2941 cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt
     3001 tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg
     3061 cgtagtttta cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg
     3121 atttttccta aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa
     3181 taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa
     3241 aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct
     3301 ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat
     3361 gggcactggc aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac
     3421 agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt
     3481 tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg
     3541 cggactattc gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta
     3601 tagtgaagga agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa
     3661 taatcacgta gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt
     3721 aaaagctgaa ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc
     3781 agacaaagtc gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc
     3841 tgcgattgcg attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat
     3901 catttcttcc ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat
     3961 caatgcgatc caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa
     4021 tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac
     4081 ttctaatgta agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat
     4141 catcaatcaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt
     4201 cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt
     4261 aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt
     4321 gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact
     4381 tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg
     4441 ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa
     4501 tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta
     4561 ttttggaaat gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc
     4621 tggagcattt ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc
     4681 tcgcaaagtt cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac
     4741 gacaactttt tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc
     4801 attgtctact tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt
     4861 ttttacgtac ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc
     4921 tgcttctttg atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc
     4981 gttcccttcg attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc
     5041 ttgttcatct ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt
     5101 atcgtcggct ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat
     5161 catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact
     5221 gcatgaaatt tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc
     5281 gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat
     5341 taaaaaggtc gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac
     5401 gatcaagaca tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa
     5461 gcgttcgact gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt
     5521 tggcaatttg actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt
     5581 tgtgtaatca acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc
     5641 agtttgctga tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg
     5701 ctgatcgatt acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc
     5761 ttttttgaaa gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata
     5821 ttcttgattc atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa
     5881 cgattcgatt tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc
     5941 gtcttggatc acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat
     6001 gtacttgtca cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg
     6061 catttgcggt ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact
     6121 ctttccagtt tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg
     6181 tcctctactt tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac
     6241 aaacattgat cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt
     6301 atagtttcac tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa
     6361 cctttcagtg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc
     6421 gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg
     6481 ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg
     6541 atattgtttg tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa
     6601 cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc
     6661 gtcactgtat catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta
     6721 ttgacagaag ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc
     6781 aatgtatgac ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag
     6841 ccatagcggt tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt
     6901 tcagcgtaag gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt
     6961 gattcaaacc cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt
     7021 cctcgcaata aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag
     7081 gttttaagtg taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg
     7141 actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt
     7201 gctgcgacca ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg
     7261 cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg
     7321 cgaacatctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga
     7381 aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta
     7441 tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta
     7501 ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac
     7561 aaggagtaac aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc
     7621 tattttaaag gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca
     7681 acgatttggt atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag
     7741 tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct
     7801 attaggaaat tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat
     7861 ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt attagaaatt
     7921 tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc
     7981 cactattcaa aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag
     8041 ctttccaaac atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac
     8101 tagcatttcc tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga
     8161 gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa
     8221 gcaacaattt caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat
     8281 gtctatgata ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc
     8341 gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc
     8401 gatgccgact attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat
     8461 ggtttgctcg agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca
     8521 aatggaacct actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt
     8581 agaaatacaa ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga
     8641 ggattcaatc gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa
     8701 acggaattga cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat
     8761 tatcgtgatc gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa
     8821 gtgatcatga tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt
     8881 tggaccattt tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat
     8941 atgctgacaa gctttattac acgcatccct tctgcgattt taagtactgg attgatgact
     9001 ttagcacttc tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag
     9061 aaagaatatg aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa
     9121 aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc
     9181 gccaaacgat agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc
     9241 ataaattcgt ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca
     9301 acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt
     9361 tctccagtgt ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt
     9421 acgtaatgtg gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact
     9481 gtttgttcga tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc
     9541 atattgatga agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc
     9601 attgtcaatt tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa
     9661 tcttcatgga cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca
     9721 tagttatcta agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca
     9781 ccatgtgcgc ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt
     9841 ccgcttactg gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca
     9901 tctgcacttg gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt
     9961 ccggaaacaa aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct
    10021 aaagaaacat ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca
    10081 aaatctggtt cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca
    10141 tcgctgccga ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc
    10201 ttcatcgcat agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag
    10261 atctttccat ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga
    10321 acgacatcca tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc
    10381 cctgcatagt tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata
    10441 tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca
    10501 tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt
    10561 gcttctactt ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct
    10621 tcgtgttcat gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt
    10681 ttcttatctg tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag
    10741 atgctgataa acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat
    10801 aaaggaacat ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc
    10861 attatgagtg ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat
    10921 gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg
    10981 gatactgacc agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga
    11041 ttgggtactt cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa
    11101 gaacaatggc tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg
    11161 ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt
    11221 tctctaccga tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt
    11281 gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc
    11341 aatcctttag gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg
    11401 atcacaggat ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat
    11461 cttcatagtt tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa
    11521 atcagcgaag cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag
    11581 gcatgagtca tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat
    11641 caaatgggga ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc
    11701 ttattggtac atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc
    11761 gttctggaag taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc
    11821 catccaacta tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga
    11881 actgaagaca aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat
    11941 tcgcaacgat tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg
    12001 tacgcttctt tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt
    12061 gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa
    12121 agtaatgcac ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa
    12181 tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg
    12241 tgtcaaaccg atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct
    12301 aataggcatg cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt
    12361 aagcgattta tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa
    12421 taatttgcaa cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc
    12481 acctggaagt aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc
    12541 gattcgagaa gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc
    12601 cggcacttat gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga
    12661 aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc
    12721 taaactagaa aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc
    12781 tttttttgat ttggatactg attttcaccg aacttgttat gaaattgctg gaaaacgaga
    12841 aatttgggat tggctagaaa gttatagtac gcacttgaac cgtttcagat ggttacgttt
    12901 gacgatttca gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc
    12961 aatgattgat cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat
    13021 tatcgaggag caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga
    13081 cagacccatc aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa
    13141 acggcaaaat agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg
    13201 attgcacacc gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    13261 gccaagtaaa aatagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    13321 gcttttgaac accgttttcc aagaggtcgt gagcctgccg ttcatctccg agtcacaagg
    13381 aacaatttca aaaaacagct tctcatgttt gttgaatatt ttgacttgtg accgaggagt
    13441 tgacaggaga acatcgttta ttcggaaaaa aaggactgtg acacgacttt tgtcacagtc
    13501 tctttatttg taatctaaat catactccgt acatcttttt tctatttttt ttatttcttc
    13561 taagatccct tggctacgaa tttcattata tggccaaagt aagactgcct ctaatacata
    13621 aggcgaaaaa tcgctttgat tcatctcttt tgaaaaactc atcgctgcta tctcgctcaa
    13681 tgcacgaatg tttgattgat gtgtataaaa aggcaaccgc cacagcacaa ttttttcgct
    13741 ttgcgtataa attcccttaa cagaagcttc gatgtggtga aatagttcat gtccaataat
    13801 caaagcttgc gctgtttctt tagagatcga agctagtcct gaacatttta atttttctaa
    13861 cggctccgtc atcaatcgaa tcccttcatc ttctgcataa gttgcaaaag tgattcgttc
    13921 gtttatttgc tctgattcac aggaaacgat tggaacagcc atttgttcac aaatttctcc
    13981 tacatcggca aaagggtact tttttttgat gctacatgcc gtttcgatac cacaatcgat
    14041 tgattgttgt ataagaattt tctgcttgga caaaggaatc tttttccaaa ataaatctcg
    14101 ccgaaactgg tagaatcccc aaatttcttc ggggatctgt tgtaattcgt ttagtaactc
    14161 ttctaactta tgtttgtttg atatttccgg caacgacgat cacttcttcc tccggttcca
    14221 tagtagatac ttccagatcc atcaaaagct gtagttgttc cagatccgtc gctgtaaaat
    14281 cagagactct tgctcctaag cagagataaa attctggtcg ggcaatcaat tcagtttgat
    14341 aaactgccat atcttcccaa agttgttcga cagctgtcat ataattggct gcagttgttt
    14401 tcatacatga agcatgtcca cgttggacct tgataaatcc tttttgatcg atgatccgaa
    14461 gacttccagc attttttgaa cgattctgtt cgccgcatac ataaaagaaa ggtgtatttt
    14521 ccaataagca aacttcattt ttatttaatc gcatatcctc tgcagcaagc tctagtgctt
    14581 cttctgttgt gatctccttg gttagatcgg ttgctttcac ttccgtagaa ccagtagcga
    14641 tagccgttac ttttgaagtc tgtggatcga tttctacgtg gacttcgatc gattctggtg
    14701 tagcgcctga ttcaatcgct ttattcatcg cttcattttt caacgatcgt atatcttctt
    14761 tacttggcga agggatgata cgttctacaa catctcggac cattgcaagt gctacaccaa
    14821 tagaagaaat cacttcggcg ttttcaggaa tcgaatattt cacacccatt ttattactaa
    14881 agtaagtaat caatgaggca gcgccgcccc ctacacctac aagagaaatc tgatctttct
    14941 ccaagtgata cttgtctgcc aattctaaaa tcaccggttc aatcttagca taggattttt
    15001 ccatgatctg ttctgcgatg tcttcaaccg tcgtatgaca ataatcagct aatgcttgga
    15061 tggcttttcg tgcagatggg acatttccat aagaaaaatg ttcttcttgc actaatccaa
    15121 ggacattggc tgcacatgta tttgtaatag tcacttcttc tccgtcttcc atcactacct
    15181 tgacgtaatc agcagggtct ccttctttag gagagaaaaa ttcaacctta ggacctttga
    15241 ttttttctgt ctccgtaaat actgcataat ctagaccggc aatatgtgca gaacgtggac
    15301 cgacatcgat gattcctgac tgattagccc gtaccatcga accgcctgcc acacctagaa
    15361 cccgaacatc taatgaagag atatatgttg ggtggccacc tacaattgaa taatcgattg
    15421 ctggacgtcc atttttaatg accccgatat tggttgttgt tccgcctact tcaaaataaa
    15481 caccattaga cgctctcaag tacatcaaag agcccatgac tgatgctgct ggaccggaaa
    15541 gcatggttaa aacaggtcgt tttttcattt cattgatttc cattactccg ccgtctccgc
    15601 gcataatcat caaagaaaca ttgacccctg cttctcgtac ggaatcttct gtagaggttg
    15661 ccgtatctag catttttggt aaaatagatg cattgattgc tgctgtccga gttcttcttg
    15721 tcaatccata taattttgtg atgtcagaag ccatcgttgt agggatcgat tttaccgatg
    15781 ctgcttcata aacaactcgt tctggttccc cattatctac tccaaatgcc atagaagaaa
    15841 ccaagacctc cgctctttct ctttctaaag aagaaatcgt ttctgaaaca cggtctacat
    15901 ttaagtgttt gacaggtaag aatgcattga caatctctat ttttttctta ttccctaaat
    15961 cgatatcgtt caatctagtt tgtcgttttg ctaaaaaacc ttctaatcca ccttttgcca
    16021 tcccgattac gccgactttt gctacgtctc cttcaatcag tgcattagtc gcttgtgtgg
    16081 tagaatgtgc aacaaacaca acatcttctg gactgatatt attttccagc aaacaatttt
    16141 gaaaagactg aacgacacct gcggctactc cacggacatc atcgtgggtt gttttcactg
    16201 aagattttcc gatgatttcg tgcgtcgcat tatcgattgc gactgctttc gtatgtgtac
    16261 cgccaacatc aattcccatt cgcacaaaac gtttcatttt cttttcctcc attcatctaa
    16321 tcgtttttat cgacgttttg tttactaggc gttcccgtac aataagaacg tgatcaggca
    16381 taagatcgct ccgatgatcc aaccagttgg gatggatttc ttcatatatt ctcgagaagt
    16441 tacttttgta taacctaatc cccaagcaac ccaagattgt gtaatatcta aatgctgagg
    16501 agcaatcgtt gtgattgcaa ataacgggaa caaaaaggct ggcgacatcg ttggattaac
    16561 agctaatacg acagctaaaa tagctgagcc tgaaccgact agattcattg gaccgcggaa
    16621 aaatcctagt ggtgtcaata tagcaaatac gattgccaag actaatggtg attgcggtat
    16681 ccaatctcca aaaatagctg aaaaataagg agaagcatac gcagctgaat tattgaacat
    16741 cgctaaggtc atcaagaaac caaccattgg cgcaacatct actgcgccat ctgtaaataa
    16801 tttcgcaaac tgacgacaga tttctttata acctccgcgc aattttccag taaacagcaa
    16861 tgcccagata cctgctagaa taaaacctaa gatgattgga agatctaaaa gaacgacccc
    16921 taaaactggt aataatacgg ctaaccatga gatcattggt gcgtcgccac cttcagctgg
    16981 tacgtttgct gccattgcat agcgtttctt tttgttcatt gatatattag cgaccgttag
    17041 taccacaact aagcttacaa tcatcccaat cataccaatt tggaaatagt cattataagt
    17101 atagctttca gccgctggat tgaaaccagc ataaatcgtt tgatattgtt tgaaattgac
    17161 gatattagca aagattccag ccataatcga gcccataaat gagaacagtg caaccggtac
    17221 tgaaatacct aatgacatca agattggcaa cacaataact gcaatagaaa tcactggtcc
    17281 gattcctgtc atcgacatga atataacagc ggttacaata catagtaagc tcatcgtgat
    17341 tcttggcttg tcccctccaa gttcaactac ctttcgaatc agggtagctg cgattccgct
    17401 atctactaga acacgcccga aaaaggcacc aaagaagaca tttaccaaga tcgatttggc
    17461 gtactcagct ggccccgtcg tatagatatg cgtcaacaca tcaattactc cttgcccttc
    17521 catcgccggg tttggtgaaa acgtgtttcc gactagcgct aatcccatcc atacagtagc
    17581 catgatggca aagccgacca tcaagttgta gccttttaca cagtaccaga ccatgaaaaa
    17641 gaatgagata actaacaaaa taccaataat aatatcactc atgttattcc tccgtccatt
    17701 ttgtttttct gataagcaaa ctaatatatc agtcaaactc attctaactt atgtagttag
    17761 cgctttcatt aaagggtgac cataaatgtt cagttttcaa ttaaaaaacc ttaattatgc
    17821 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
    17881 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
    17941 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
    18001 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
    18061 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
    18121 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
    18181 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
    18241 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
    18301 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
    18361 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
    18421 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
    18481 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
    18541 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
    18601 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
    18661 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
    18721 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
    18781 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
    18841 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
    18901 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
    18961 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
    19021 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
    19081 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
    19141 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
    19201 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
    19261 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
    19321 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
    19381 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
    19441 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
    19501 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
    19561 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
    19621 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
    19681 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
    19741 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
    19801 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
    19861 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
    19921 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
    19981 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
    20041 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
    20101 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
    20161 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
    20221 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
    20281 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
    20341 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
    20401 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
    20461 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
    20521 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
    20581 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
    20641 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
    20701 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
    20761 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
    20821 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
    20881 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
    20941 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
    21001 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
    21061 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
    21121 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
    21181 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
    21241 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
    21301 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
    21361 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
    21421 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
    21481 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
    21541 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
    21601 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
    21661 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
    21721 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
    21781 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
    21841 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
    21901 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
    21961 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
    22021 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
    22081 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
    22141 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
    22201 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
    22261 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
    22321 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
    22381 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
    22441 actattagaa aatgtccagc tttcggaaaa aatcgcccat ggtaatccta gtggaatcga
    22501 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
    22561 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
    22621 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
    22681 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
    22741 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
    22801 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
    22861 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
    22921 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
    22981 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
    23041 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
    23101 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
    23161 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
    23221 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
    23281 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
    23341 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
    23401 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
    23461 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
    23521 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
    23581 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
    23641 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
    23701 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
    23761 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
    23821 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
    23881 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
    23941 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
    24001 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
    24061 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
    24121 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
    24181 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
    24241 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
    24301 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
    24361 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
    24421 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
    24481 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
    24541 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
    24601 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
    24661 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
    24721 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
    24781 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
    24841 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
    24901 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
    24961 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
    25021 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
    25081 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
    25141 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
    25201 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
    25261 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
    25321 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
    25381 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
    25441 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
    25501 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
    25561 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
    25621 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
    25681 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
    25741 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
    25801 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
    25861 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
    25921 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
    25981 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
    26041 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
    26101 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    26161 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    26221 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
    26281 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
    26341 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
    26401 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
    26461 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
    26521 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
    26581 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
    26641 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
    26701 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
    26761 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
    26821 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
    26881 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
    26941 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
    27001 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
    27061 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
    27121 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
    27181 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
    27241 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
    27301 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
    27361 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
    27421 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
    27481 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
    27541 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
    27601 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
    27661 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
    27721 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
    27781 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    27841 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    27901 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    27961 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    28021 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    28081 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    28141 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    28201 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    28261 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    28321 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    28381 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    28441 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    28501 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    28561 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    28621 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    28681 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    28741 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    28801 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    28861 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    28921 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    28981 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    29041 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    29101 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    29161 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    29221 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    29281 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    29341 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    29401 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    29461 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    29521 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    29581 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    29641 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    29701 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    29761 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    29821 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    29881 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    29941 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    30001 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    30061 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    30121 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    30181 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    30241 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    30301 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    30361 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    30421 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    30481 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    30541 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    30601 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    30661 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    30721 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    30781 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    30841 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    30901 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    30961 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    31021 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    31081 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    31141 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    31201 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    31261 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    31321 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    31381 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    31441 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    31501 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    31561 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    31621 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    31681 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    31741 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    31801 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    31861 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    31921 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    31981 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    32041 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    32101 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    32161 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    32221 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    32281 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    32341 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    32401 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    32461 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    32521 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    32581 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    32641 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    32701 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    32761 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    32821 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    32881 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    32941 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    33001 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    33061 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    33121 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    33181 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    33241 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    33301 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    33361 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    33421 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    33481 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    33541 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    33601 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    33661 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    33721 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    33781 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    33841 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    33901 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    33961 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    34021 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    34081 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    34141 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    34201 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    34261 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    34321 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    34381 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    34441 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    34501 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    34561 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    34621 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    34681 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    34741 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    34801 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    34861 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    34921 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    34981 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    35041 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    35101 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    35161 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    35221 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    35281 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    35341 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    35401 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    35461 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    35521 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    35581 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    35641 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    35701 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    35761 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    35821 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    35881 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    35941 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    36001 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    36061 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    36121 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    36181 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    36241 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    36301 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    36361 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    36421 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    36481 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    36541 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    36601 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    36661 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    36721 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    36781 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    36841 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    36901 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    36961 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    37021 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    37081 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    37141 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    37201 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    37261 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    37321 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    37381 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    37441 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    37501 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    37561 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    37621 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    37681 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    37741 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    37801 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    37861 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    37921 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    37981 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    38041 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    38101 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    38161 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    38221 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    38281 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    38341 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    38401 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    38461 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    38521 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    38581 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    38641 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    38701 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    38761 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    38821 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    38881 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    38941 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    39001 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    39061 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    39121 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    39181 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    39241 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    39301 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    39361 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    39421 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    39481 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    39541 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    39601 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    39661 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    39721 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    39781 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    39841 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    39901 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    39961 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    40021 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    40081 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    40141 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    40201 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    40261 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    40321 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    40381 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    40441 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    40501 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    40561 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    40621 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    40681 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    40741 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    40801 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    40861 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    40921 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    40981 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    41041 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    41101 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    41161 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    41221 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    41281 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    41341 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    41401 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    41461 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    41521 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    41581 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    41641 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    41701 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    41761 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    41821 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    41881 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    41941 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    42001 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    42061 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    42121 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    42181 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    42241 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    42301 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    42361 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    42421 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    42481 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    42541 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    42601 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    42661 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    42721 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    42781 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    42841 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    42901 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    42961 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    43021 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    43081 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    43141 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    43201 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    43261 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    43321 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    43381 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    43441 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    43501 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    43561 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    43621 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    43681 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    43741 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    43801 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    43861 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    43921 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    43981 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    44041 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    44101 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    44161 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    44221 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    44281 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    44341 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    44401 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    44461 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    44521 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    44581 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    44641 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    44701 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    44761 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    44821 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    44881 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    44941 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    45001 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    45061 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    45121 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    45181 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    45241 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    45301 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    45361 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    45421 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    45481 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    45541 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    45601 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    45661 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    45721 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    45781 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    45841 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    45901 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    45961 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    46021 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    46081 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    46141 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    46201 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    46261 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    46321 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    46381 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    46441 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    46501 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    46561 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    46621 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    46681 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    46741 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    46801 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    46861 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    46921 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    46981 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    47041 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    47101 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    47161 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    47221 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    47281 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    47341 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    47401 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    47461 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    47521 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    47581 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    47641 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    47701 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    47761 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    47821 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    47881 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    47941 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    48001 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    48061 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    48121 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    48181 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    48241 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    48301 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    48361 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    48421 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    48481 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    48541 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    48601 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    48661 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    48721 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    48781 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    48841 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    48901 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    48961 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    49021 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    49081 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    49141 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    49201 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    49261 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    49321 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    49381 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    49441 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    49501 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    49561 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    49621 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    49681 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    49741 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    49801 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    49861 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    49921 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    49981 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    50041 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    50101 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    50161 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    50221 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    50281 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    50341 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    50401 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    50461 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    50521 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    50581 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    50641 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    50701 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    50761 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    50821 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    50881 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    50941 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    51001 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    51061 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    51121 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    51181 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    51241 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    51301 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    51361 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    51421 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    51481 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    51541 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    51601 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    51661 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    51721 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    51781 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    51841 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    51901 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    51961 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    52021 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    52081 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    52141 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    52201 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    52261 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    52321 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    52381 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    52441 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    52501 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    52561 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    52621 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    52681 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    52741 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    52801 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    52861 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    52921 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    52981 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    53041 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    53101 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    53161 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    53221 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    53281 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    53341 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    53401 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    53461 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    53521 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    53581 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    53641 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    53701 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    53761 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    53821 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    53881 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    53941 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    54001 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    54061 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    54121 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    54181 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    54241 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    54301 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    54361 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    54421 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    54481 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    54541 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    54601 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    54661 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    54721 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    54781 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    54841 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    54901 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    54961 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    55021 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    55081 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    55141 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    55201 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    55261 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    55321 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    55381 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    55441 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    55501 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    55561 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    55621 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    55681 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    55741 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    55801 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    55861 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    55921 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    55981 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    56041 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    56101 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    56161 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    56221 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    56281 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    56341 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    56401 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    56461 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    56521 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    56581 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    56641 tccttatggg caaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    56701 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    56761 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    56821 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    56881 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    56941 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    57001 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    57061 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    57121 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    57181 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    57241 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    57301 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    57361 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    57421 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    57481 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    57541 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    57601 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    57661 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    57721 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    57781 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    57841 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    57901 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    57961 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    58021 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    58081 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    58141 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    58201 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    58261 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    58321 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    58381 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    58441 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    58501 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    58561 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    58621 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    58681 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    58741 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    58801 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    58861 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    58921 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    58981 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    59041 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    59101 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    59161 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    59221 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    59281 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    59341 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    59401 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    59461 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    59521 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    59581 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    59641 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    59701 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    59761 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    59821 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    59881 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    59941 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    60001 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    60061 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    60121 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    60181 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    60241 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    60301 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    60361 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    60421 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    60481 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    60541 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    60601 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    60661 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    60721 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    60781 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    60841 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    60901 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    60961 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    61021 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    61081 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    61141 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    61201 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    61261 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    61321 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    61381 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    61441 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    61501 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    61561 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    61621 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    61681 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    61741 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    61801 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    61861 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    61921 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    61981 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    62041 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    62101 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    62161 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    62221 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    62281 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    62341 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    62401 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    62461 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    62521 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    62581 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    62641 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    62701 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    62761 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    62821 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    62881 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    62941 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    63001 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    63061 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    63121 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    63181 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    63241 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    63301 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    63361 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    63421 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    63481 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    63541 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    63601 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    63661 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    63721 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    63781 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    63841 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    63901 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    63961 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    64021 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    64081 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    64141 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    64201 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    64261 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    64321 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    64381 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    64441 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    64501 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    64561 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    64621 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    64681 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    64741 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    64801 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    64861 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    64921 agcttctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    64981 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    65041 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    65101 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    65161 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    65221 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    65281 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    65341 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    65401 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    65461 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    65521 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    65581 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    65641 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    65701 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    65761 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    65821 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    65881 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    65941 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    66001 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    66061 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    66121 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    66181 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    66241 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    66301 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    66361 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    66421 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    66481 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    66541 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    66601 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    66661 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    66721 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    66781 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    66841 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    66901 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    66961 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    67021 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    67081 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    67141 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    67201 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    67261 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    67321 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    67381 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    67441 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    67501 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    67561 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    67621 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    67681 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    67741 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    67801 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    67861 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    67921 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    67981 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    68041 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    68101 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    68161 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    68221 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    68281 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    68341 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    68401 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    68461 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    68521 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    68581 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    68641 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    68701 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    68761 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    68821 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    68881 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    68941 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    69001 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    69061 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    69121 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    69181 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    69241 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    69301 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    69361 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    69421 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    69481 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    69541 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    69601 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    69661 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    69721 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    69781 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    69841 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    69901 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    69961 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    70021 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    70081 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    70141 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    70201 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    70261 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    70321 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    70381 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    70441 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    70501 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    70561 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    70621 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    70681 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    70741 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    70801 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    70861 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    70921 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    70981 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    71041 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    71101 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    71161 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    71221 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    71281 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    71341 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    71401 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    71461 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    71521 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    71581 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    71641 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    71701 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    71761 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    71821 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    71881 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    71941 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    72001 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    72061 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    72121 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    72181 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    72241 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    72301 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    72361 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    72421 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    72481 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    72541 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    72601 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    72661 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    72721 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    72781 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    72841 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    72901 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    72961 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    73021 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    73081 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    73141 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    73201 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    73261 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    73321 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    73381 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    73441 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    73501 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    73561 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    73621 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    73681 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    73741 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    73801 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    73861 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    73921 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    73981 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    74041 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    74101 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    74161 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    74221 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    74281 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    74341 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    74401 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    74461 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    74521 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    74581 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    74641 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    74701 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    74761 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    74821 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    74881 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    74941 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    75001 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    75061 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    75121 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    75181 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    75241 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    75301 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    75361 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    75421 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    75481 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    75541 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    75601 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    75661 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    75721 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    75781 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    75841 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    75901 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    75961 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    76021 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    76081 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    76141 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    76201 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    76261 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    76321 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    76381 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    76441 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    76501 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    76561 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    76621 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    76681 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    76741 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    76801 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    76861 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    76921 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    76981 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    77041 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    77101 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    77161 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    77221 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    77281 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    77341 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    77401 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    77461 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    77521 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    77581 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    77641 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    77701 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    77761 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    77821 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    77881 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    77941 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    78001 aaactgcctc ctt
//
LOCUS       DACDEA010000004        72952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDEA010000004 DACDEA010000000
VERSION     DACDEA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.004"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            317..3151
                     /locus_tag="IV136_000273"
     CDS             317..3151
                     /locus_tag="IV136_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8464293.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3343..3834
                     /locus_tag="IV136_000274"
     CDS             3343..3834
                     /locus_tag="IV136_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8464294.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3937..4926
                     /gene="manX"
                     /locus_tag="IV136_000275"
     CDS             3937..4926
                     /gene="manX"
                     /locus_tag="IV136_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8464295.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4959..5762
                     /locus_tag="IV136_000276"
     CDS             4959..5762
                     /locus_tag="IV136_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8464296.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5783..6697
                     /locus_tag="IV136_000277"
     CDS             5783..6697
                     /locus_tag="IV136_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8464297.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6780..7154
                     /locus_tag="IV136_000278"
     CDS             6780..7154
                     /locus_tag="IV136_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8464298.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7293..7538
                     /locus_tag="IV136_000279"
     CDS             7293..7538
                     /locus_tag="IV136_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8464299.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7710..8774
                     /locus_tag="IV136_000280"
     CDS             7710..8774
                     /locus_tag="IV136_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8464300.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9020..9445
                     /locus_tag="IV136_000281"
     CDS             9020..9445
                     /locus_tag="IV136_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8464301.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9438..9887
                     /gene="nusB"
                     /locus_tag="IV136_000282"
     CDS             9438..9887
                     /gene="nusB"
                     /locus_tag="IV136_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8464302.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9984..10838
                     /locus_tag="IV136_000283"
     CDS             9984..10838
                     /locus_tag="IV136_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8464303.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10839..12176
                     /locus_tag="IV136_000284"
     CDS             10839..12176
                     /locus_tag="IV136_000284"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8464304.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12181..12405
                     /locus_tag="IV136_000285"
     CDS             12181..12405
                     /locus_tag="IV136_000285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8464305.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12407..13297
                     /locus_tag="IV136_000286"
     CDS             12407..13297
                     /locus_tag="IV136_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8464306.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13306..14124
                     /locus_tag="IV136_000287"
     CDS             13306..14124
                     /locus_tag="IV136_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8464307.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14231..14680
                     /locus_tag="IV136_000288"
     CDS             14231..14680
                     /locus_tag="IV136_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8464308.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14698..16380
                     /gene="recN"
                     /locus_tag="IV136_000289"
     CDS             14698..16380
                     /gene="recN"
                     /locus_tag="IV136_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8464309.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16435..16551)
                     /locus_tag="IV136_000290"
     CDS             complement(16435..16551)
                     /locus_tag="IV136_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8464310.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16624..17580)
                     /locus_tag="IV136_000291"
     CDS             complement(16624..17580)
                     /locus_tag="IV136_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8464311.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17751..18119
                     /locus_tag="IV136_000292"
     CDS             17751..18119
                     /locus_tag="IV136_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8464312.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18289..18720
                     /gene="mraZ"
                     /locus_tag="IV136_000293"
     CDS             18289..18720
                     /gene="mraZ"
                     /locus_tag="IV136_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8464313.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18741..19700
                     /gene="rsmH"
                     /locus_tag="IV136_000294"
     CDS             18741..19700
                     /gene="rsmH"
                     /locus_tag="IV136_000294"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8464314.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19705..20106
                     /gene="ftsL"
                     /locus_tag="IV136_000295"
     CDS             19705..20106
                     /gene="ftsL"
                     /locus_tag="IV136_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8464315.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20107..22293
                     /locus_tag="IV136_000296"
     CDS             20107..22293
                     /locus_tag="IV136_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8464316.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22319..23281
                     /locus_tag="IV136_000297"
     CDS             22319..23281
                     /locus_tag="IV136_000297"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8464317.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23295..24656
                     /locus_tag="IV136_000298"
     CDS             23295..24656
                     /locus_tag="IV136_000298"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8464318.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24682..25770
                     /gene="murG"
                     /locus_tag="IV136_000299"
     CDS             24682..25770
                     /gene="murG"
                     /locus_tag="IV136_000299"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8464319.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25785..27005
                     /locus_tag="IV136_000300"
     CDS             25785..27005
                     /locus_tag="IV136_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8464320.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27161..28486
                     /gene="ftsA"
                     /locus_tag="IV136_000301"
     CDS             27161..28486
                     /gene="ftsA"
                     /locus_tag="IV136_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8464321.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28509..29750
                     /gene="ftsZ"
                     /locus_tag="IV136_000302"
     CDS             28509..29750
                     /gene="ftsZ"
                     /locus_tag="IV136_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8464322.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29767..30444
                     /locus_tag="IV136_000303"
     CDS             29767..30444
                     /locus_tag="IV136_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8464323.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30458..31057
                     /locus_tag="IV136_000304"
     CDS             30458..31057
                     /locus_tag="IV136_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8464324.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31072..31359
                     /locus_tag="IV136_000305"
     CDS             31072..31359
                     /locus_tag="IV136_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8464325.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31408..32190
                     /locus_tag="IV136_000306"
     CDS             31408..32190
                     /locus_tag="IV136_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8464326.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32211..32918
                     /locus_tag="IV136_000307"
     CDS             32211..32918
                     /locus_tag="IV136_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8464327.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33180..35978
                     /gene="ileS"
                     /locus_tag="IV136_000308"
     CDS             33180..35978
                     /gene="ileS"
                     /locus_tag="IV136_000308"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8464328.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36027..37553)
                     /locus_tag="IV136_000309"
     CDS             complement(36027..37553)
                     /locus_tag="IV136_000309"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8464329.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37568..38215)
                     /locus_tag="IV136_000310"
     CDS             complement(37568..38215)
                     /locus_tag="IV136_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8464330.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38399..38728)
                     /locus_tag="IV136_000311"
     CDS             complement(38399..38728)
                     /locus_tag="IV136_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8464331.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38905..39633
                     /gene="sfsA"
                     /locus_tag="IV136_000312"
     CDS             38905..39633
                     /gene="sfsA"
                     /locus_tag="IV136_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8464332.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39649..40662
                     /locus_tag="IV136_000313"
     CDS             39649..40662
                     /locus_tag="IV136_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8464333.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40662..41939
                     /locus_tag="IV136_000314"
     CDS             40662..41939
                     /locus_tag="IV136_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8464334.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42002..44704
                     /locus_tag="IV136_000315"
     CDS             42002..44704
                     /locus_tag="IV136_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8464335.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44856..45173
                     /locus_tag="IV136_000316"
     CDS             44856..45173
                     /locus_tag="IV136_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8464336.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45203..45523
                     /locus_tag="IV136_000317"
     CDS             45203..45523
                     /locus_tag="IV136_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8464337.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45631..47091
                     /locus_tag="IV136_000318"
     CDS             45631..47091
                     /locus_tag="IV136_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8464338.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47159..47380
                     /locus_tag="IV136_000319"
     CDS             47159..47380
                     /locus_tag="IV136_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464339.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47411..47593
                     /locus_tag="IV136_000320"
     CDS             47411..47593
                     /locus_tag="IV136_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8464340.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47593..48006
                     /locus_tag="IV136_000321"
     CDS             47593..48006
                     /locus_tag="IV136_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8464341.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48129..49310
                     /locus_tag="IV136_000322"
     CDS             48129..49310
                     /locus_tag="IV136_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464342.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49699..49772
                     /locus_tag="IV136_000323"
     tRNA            49699..49772
                     /locus_tag="IV136_000323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49733..49735,aa:Arg,seq:cct)
     gene            complement(49809..50009)
                     /locus_tag="IV136_000324"
     CDS             complement(49809..50009)
                     /locus_tag="IV136_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8464343.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(50314..51546)
                     /locus_tag="IV136_000325"
     CDS             complement(50314..51546)
                     /locus_tag="IV136_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8464344.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            51800..52369
                     /locus_tag="IV136_000326"
     CDS             51800..52369
                     /locus_tag="IV136_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8464345.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(52547..52987)
                     /locus_tag="IV136_000327"
     CDS             complement(52547..52987)
                     /locus_tag="IV136_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8464346.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            53145..53909
                     /gene="map"
                     /locus_tag="IV136_000328"
     CDS             53145..53909
                     /gene="map"
                     /locus_tag="IV136_000328"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8464347.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            53941..54864
                     /locus_tag="IV136_000329"
     CDS             53941..54864
                     /locus_tag="IV136_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8464348.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            54940..56079
                     /locus_tag="IV136_000330"
     CDS             54940..56079
                     /locus_tag="IV136_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8464349.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            56072..56872
                     /locus_tag="IV136_000331"
     CDS             56072..56872
                     /locus_tag="IV136_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8464350.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            56872..57699
                     /locus_tag="IV136_000332"
     CDS             56872..57699
                     /locus_tag="IV136_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8464351.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            57677..58411
                     /locus_tag="IV136_000333"
     CDS             57677..58411
                     /locus_tag="IV136_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8464352.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            58511..59377
                     /gene="rfbA"
                     /locus_tag="IV136_000334"
     CDS             58511..59377
                     /gene="rfbA"
                     /locus_tag="IV136_000334"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8464353.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            59391..59963
                     /gene="rfbC"
                     /locus_tag="IV136_000335"
     CDS             59391..59963
                     /gene="rfbC"
                     /locus_tag="IV136_000335"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8464354.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            59985..61013
                     /gene="rfbB"
                     /locus_tag="IV136_000336"
     CDS             59985..61013
                     /gene="rfbB"
                     /locus_tag="IV136_000336"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8464355.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            61111..61962
                     /gene="rfbD"
                     /locus_tag="IV136_000337"
     CDS             61111..61962
                     /gene="rfbD"
                     /locus_tag="IV136_000337"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8464356.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            61997..64030
                     /locus_tag="IV136_000338"
     CDS             61997..64030
                     /locus_tag="IV136_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464357.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            64074..65354
                     /locus_tag="IV136_000339"
     CDS             64074..65354
                     /locus_tag="IV136_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8464358.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            65564..66370
                     /locus_tag="IV136_000340"
     CDS             65564..66370
                     /locus_tag="IV136_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464359.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            66382..67602
                     /locus_tag="IV136_000341"
     CDS             66382..67602
                     /locus_tag="IV136_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464360.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            67592..69178
                     /locus_tag="IV136_000342"
     CDS             67592..69178
                     /locus_tag="IV136_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8464361.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            69217..71355
                     /locus_tag="IV136_000343"
     CDS             69217..71355
                     /locus_tag="IV136_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8464362.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(71723..72706)
                     /locus_tag="IV136_000344"
     CDS             complement(71723..72706)
                     /locus_tag="IV136_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8464363.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT        23846 a        12384 c        15506 g        21216 t
ORIGIN      
        1 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
       61 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      121 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      181 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
      241 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
      301 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
      361 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
      421 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
      481 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
      541 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
      601 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
      661 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
      721 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
      781 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
      841 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
      901 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
      961 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1021 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1081 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1141 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1201 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     1261 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     1321 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     1381 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     1441 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     1501 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     1561 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     1621 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     1681 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     1741 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     1801 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     1861 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     1921 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     1981 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2041 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2101 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2161 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     2221 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     2281 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     2341 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     2401 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     2461 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     2521 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     2581 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     2641 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     2701 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     2761 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     2821 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     2881 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     2941 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3001 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3061 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3121 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3181 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaaag
     3241 ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga
     3301 taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca
     3361 cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt
     3421 gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta
     3481 ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt
     3541 caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc
     3601 caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt
     3661 atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat
     3721 attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct
     3781 gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa
     3841 tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg
     3901 aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt
     3961 catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa
     4021 aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg
     4081 gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg
     4141 ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca
     4201 atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg
     4261 gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta
     4321 aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct
     4381 aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca
     4441 cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa
     4501 ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta
     4561 atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc
     4621 gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct
     4681 gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca
     4741 atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac
     4801 gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct
     4861 aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa
     4921 gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt
     4981 tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc
     5041 atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta
     5101 tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg
     5161 tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac
     5221 aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt
     5281 tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg
     5341 ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac
     5401 aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc
     5461 aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa
     5521 tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc
     5581 cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg attgctctag
     5641 gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat
     5701 caaataatgg aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt
     5761 aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg
     5821 tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca
     5881 aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga
     5941 agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc
     6001 ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt
     6061 tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg
     6121 tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc
     6181 tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg
     6241 ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga
     6301 cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt
     6361 attagctgcc cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt
     6421 aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat
     6481 caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac
     6541 attacaagac aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt
     6601 atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt
     6661 agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg
     6721 ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa
     6781 tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc
     6841 tgacagatta cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag
     6901 atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca
     6961 tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca
     7021 cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag
     7081 atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt
     7141 ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact
     7201 ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc
     7261 taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg
     7321 gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg
     7381 taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat
     7441 ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg
     7501 tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta
     7561 aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa
     7621 gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa
     7681 aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa
     7741 aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact
     7801 taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta
     7861 ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta
     7921 aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact
     7981 cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca
     8041 tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg
     8101 aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca
     8161 tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt
     8221 atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc
     8281 gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca
     8341 cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta
     8401 ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag
     8461 cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag
     8521 attatatttc aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg
     8581 gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag
     8641 gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg
     8701 aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat
     8761 tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact
     8821 ttgttataga aaatcactcg atttaatgat acactataga agaactttta agctgtaaga
     8881 gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt
     8941 ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg
     9001 caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga
     9061 tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc
     9121 taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt
     9181 aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt
     9241 agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca
     9301 ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat
     9361 ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga
     9421 agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat
     9481 tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca
     9541 acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg
     9601 tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga
     9661 ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga
     9721 tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt
     9781 taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca
     9841 acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt
     9901 tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac
     9961 aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa
    10021 aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg
    10081 gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag
    10141 aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt
    10201 ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta
    10261 tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc
    10321 gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta
    10381 aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta
    10441 tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga
    10501 tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa
    10561 atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt
    10621 ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg
    10681 atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct
    10741 ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa
    10801 ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg
    10861 tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg
    10921 tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca
    10981 gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat
    11041 tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg
    11101 aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt
    11161 tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc
    11221 ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag
    11281 cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt
    11341 tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga
    11401 gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg
    11461 aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc
    11521 cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg
    11581 ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt
    11641 tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc
    11701 gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat
    11761 tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac
    11821 agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag
    11881 tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct
    11941 tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc
    12001 aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt
    12061 taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc
    12121 attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat
    12181 atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta
    12241 gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta
    12301 agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca
    12361 gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga
    12421 cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca
    12481 tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag
    12541 aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc
    12601 cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga
    12661 tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt
    12721 cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat
    12781 cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg
    12841 tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa
    12901 gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta
    12961 tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca
    13021 acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat
    13081 tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac
    13141 ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc
    13201 cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat
    13261 gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc
    13321 gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca
    13381 gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa
    13441 attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg
    13501 ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg
    13561 ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa
    13621 atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct
    13681 agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta
    13741 ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct
    13801 ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc
    13861 gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa
    13921 aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta
    13981 agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa
    14041 gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca
    14101 cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga
    14161 ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag
    14221 cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca
    14281 agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac
    14341 tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga
    14401 tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga
    14461 aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa
    14521 aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga
    14581 attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca
    14641 ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg
    14701 ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac
    14761 gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct
    14821 ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa
    14881 tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt
    14941 gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact
    15001 ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc
    15061 ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa
    15121 tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt
    15181 tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa
    15241 aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct
    15301 gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc
    15361 aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa
    15421 caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta
    15481 gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat
    15541 gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa
    15601 gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt
    15661 gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca
    15721 gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa
    15781 caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt
    15841 ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa
    15901 gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc
    15961 aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg
    16021 atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt
    16081 gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct
    16141 caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc
    16201 gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt
    16261 tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag
    16321 attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga
    16381 gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt atttttacat
    16441 gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat
    16501 gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa
    16561 cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct
    16621 tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt
    16681 taatagaact agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat
    16741 tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat
    16801 tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc
    16861 tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc
    16921 acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt
    16981 caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc
    17041 catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc
    17101 gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa
    17161 ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac
    17221 tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa
    17281 aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa
    17341 aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt
    17401 acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg
    17461 atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg
    17521 gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat
    17581 gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga
    17641 atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt
    17701 aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat
    17761 tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat
    17821 ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga
    17881 ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct
    17941 taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat
    18001 attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc
    18061 tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa
    18121 aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa
    18181 aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg
    18241 ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt
    18301 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
    18361 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
    18421 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
    18481 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
    18541 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
    18601 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
    18661 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
    18721 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
    18781 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
    18841 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
    18901 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
    18961 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
    19021 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
    19081 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
    19141 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
    19201 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
    19261 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
    19321 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
    19381 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
    19441 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
    19501 tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca
    19561 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
    19621 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
    19681 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
    19741 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
    19801 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
    19861 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
    19921 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
    19981 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
    20041 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg
    20101 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
    20161 atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt
    20221 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
    20281 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
    20341 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
    20401 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
    20461 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
    20521 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
    20581 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
    20641 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
    20701 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
    20761 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
    20821 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
    20881 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
    20941 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
    21001 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
    21061 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
    21121 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
    21181 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
    21241 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
    21301 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
    21361 agtactcatt ccggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
    21421 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
    21481 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
    21541 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
    21601 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
    21661 tacggtagtg cttatggtgt tgtacctgga tacaatgtat cggcaaaaac cggaacagcc
    21721 caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt
    21781 gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct
    21841 aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg
    21901 gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta
    21961 gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg
    22021 caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat
    22081 cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac
    22141 gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt
    22201 agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact
    22261 gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat
    22321 ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat
    22381 gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg
    22441 accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat
    22501 cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct
    22561 tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat
    22621 aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat
    22681 tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa
    22741 tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct
    22801 tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt
    22861 aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt
    22921 attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa
    22981 acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc
    23041 aatttctata atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat
    23101 ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt
    23161 taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga
    23221 tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta
    23281 ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag
    23341 gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca
    23401 ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag
    23461 ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga
    23521 tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg
    23581 gtattccggt aattacggaa gttgagctag cttacgaagt atcggaatgc ccaattattg
    23641 ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg
    23701 caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg
    23761 tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca
    23821 tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat gaagcacata
    23881 ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca
    23941 tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc
    24001 aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt
    24061 atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg
    24121 gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt
    24181 atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc
    24241 gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca
    24301 atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg
    24361 ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca
    24421 aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag
    24481 gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa
    24541 acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc
    24601 caaattttga aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa
    24661 agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat
    24721 atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc
    24781 atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct
    24841 ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg
    24901 gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca
    24961 gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa
    25021 atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt
    25081 ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct
    25141 gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa
    25201 tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc
    25261 ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt
    25321 ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat
    25381 aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag
    25441 gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc
    25501 acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag
    25561 actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa
    25621 ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa
    25681 aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat
    25741 gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag
    25801 aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg
    25861 ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca
    25921 taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc
    25981 aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa
    26041 acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc
    26101 acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt
    26161 ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata
    26221 agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt
    26281 ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa
    26341 tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat
    26401 ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa
    26461 cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca
    26521 aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac
    26581 aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat
    26641 tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga
    26701 aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg
    26761 gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag
    26821 aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa
    26881 cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt
    26941 caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa
    27001 attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta
    27061 gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt
    27121 cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta
    27181 tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag
    27241 ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat
    27301 cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa
    27361 agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga
    27421 aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt
    27481 cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc
    27541 aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat
    27601 gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca
    27661 taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc
    27721 tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat
    27781 tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac
    27841 caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac
    27901 gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac
    27961 atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga
    28021 tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa
    28081 agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg
    28141 agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa
    28201 attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat
    28261 cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg
    28321 tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag
    28381 ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa
    28441 tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg
    28501 aataagatat ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca
    28561 tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag
    28621 gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa
    28681 cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag
    28741 ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag
    28801 atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg
    28861 ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca
    28921 cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa
    28981 atgtggatac attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga
    29041 caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta
    29101 tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag
    29161 tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg
    29221 tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag
    29281 ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag
    29341 atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat
    29401 ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg
    29461 aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg
    29521 ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca
    29581 gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca
    29641 attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc
    29701 atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa
    29761 gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct
    29821 cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc
    29881 gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat
    29941 aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt
    30001 ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg
    30061 ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt
    30121 tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt
    30181 ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact
    30241 atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa
    30301 ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg
    30361 gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga
    30421 acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc
    30481 aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga
    30541 aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca
    30601 ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac
    30661 gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac
    30721 aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct
    30781 tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa
    30841 acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc
    30901 gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg
    30961 tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc
    31021 tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact
    31081 attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg
    31141 gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta
    31201 gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc
    31261 ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca
    31321 cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat
    31381 tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca
    31441 gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat
    31501 gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt
    31561 cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa
    31621 agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg
    31681 tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact
    31741 ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc
    31801 tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt
    31861 ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat
    31921 agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag
    31981 atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg
    32041 aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc
    32101 agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac
    32161 agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa
    32221 ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg
    32281 aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc
    32341 gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa
    32401 aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa
    32461 gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag
    32521 ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa
    32581 aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta
    32641 agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac
    32701 aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca
    32761 gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg
    32821 taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg
    32881 atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt
    32941 cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct
    33001 acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc
    33061 acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc
    33121 ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa
    33181 tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc
    33241 caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac
    33301 aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg
    33361 gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa
    33421 aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga
    33481 ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact
    33541 tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac
    33601 gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag
    33661 ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac aaaggattga
    33721 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
    33781 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
    33841 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
    33901 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
    33961 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
    34021 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
    34081 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
    34141 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
    34201 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
    34261 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
    34321 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
    34381 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
    34441 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
    34501 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
    34561 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
    34621 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
    34681 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
    34741 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
    34801 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
    34861 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
    34921 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
    34981 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
    35041 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
    35101 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
    35161 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
    35221 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
    35281 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
    35341 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
    35401 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
    35461 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
    35521 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
    35581 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
    35641 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
    35701 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
    35761 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
    35821 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
    35881 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
    35941 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
    36001 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
    36061 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
    36121 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
    36181 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
    36241 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
    36301 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
    36361 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
    36421 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
    36481 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
    36541 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
    36601 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
    36661 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
    36721 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
    36781 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
    36841 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
    36901 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
    36961 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
    37021 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
    37081 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
    37141 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
    37201 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
    37261 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
    37321 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
    37381 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
    37441 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
    37501 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
    37561 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
    37621 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
    37681 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
    37741 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
    37801 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
    37861 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
    37921 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
    37981 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
    38041 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
    38101 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
    38161 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
    38221 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
    38281 ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga
    38341 tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg ggagggatct
    38401 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
    38461 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
    38521 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
    38581 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
    38641 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
    38701 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
    38761 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
    38821 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
    38881 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
    38941 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
    39001 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
    39061 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
    39121 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
    39181 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
    39241 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
    39301 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
    39361 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
    39421 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
    39481 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
    39541 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
    39601 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
    39661 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
    39721 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
    39781 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
    39841 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
    39901 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
    39961 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
    40021 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
    40081 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
    40141 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
    40201 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
    40261 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
    40321 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
    40381 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
    40441 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
    40501 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
    40561 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
    40621 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
    40681 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
    40741 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
    40801 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
    40861 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
    40921 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
    40981 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
    41041 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
    41101 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
    41161 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
    41221 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
    41281 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
    41341 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
    41401 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
    41461 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
    41521 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
    41581 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
    41641 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
    41701 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
    41761 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
    41821 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
    41881 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
    41941 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
    42001 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
    42061 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
    42121 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
    42181 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
    42241 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
    42301 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
    42361 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
    42421 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
    42481 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
    42541 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
    42601 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
    42661 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
    42721 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
    42781 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
    42841 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
    42901 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
    42961 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
    43021 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
    43081 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
    43141 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
    43201 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
    43261 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
    43321 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
    43381 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
    43441 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
    43501 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
    43561 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
    43621 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
    43681 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
    43741 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
    43801 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
    43861 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
    43921 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
    43981 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
    44041 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
    44101 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
    44161 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
    44221 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
    44281 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
    44341 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
    44401 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
    44461 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
    44521 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
    44581 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
    44641 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
    44701 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
    44761 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
    44821 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
    44881 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
    44941 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
    45001 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
    45061 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
    45121 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
    45181 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
    45241 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
    45301 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
    45361 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
    45421 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
    45481 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
    45541 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
    45601 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
    45661 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
    45721 gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
    45781 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
    45841 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
    45901 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
    45961 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
    46021 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
    46081 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
    46141 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
    46201 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
    46261 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
    46321 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
    46381 atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc tcaagggttg
    46441 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
    46501 ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
    46561 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
    46621 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
    46681 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
    46741 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
    46801 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
    46861 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
    46921 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
    46981 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
    47041 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
    47101 gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat
    47161 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
    47221 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
    47281 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
    47341 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
    47401 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
    47461 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
    47521 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
    47581 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
    47641 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
    47701 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
    47761 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
    47821 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
    47881 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
    47941 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
    48001 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
    48061 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
    48121 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
    48181 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
    48241 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
    48301 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
    48361 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    48421 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    48481 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    48541 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    48601 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    48661 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    48721 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    48781 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    48841 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    48901 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    48961 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    49021 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    49081 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    49141 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    49201 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    49261 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    49321 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    49381 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    49441 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    49501 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    49561 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    49621 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    49681 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    49741 ataggtcgct ggttcaattc cagtcaggaa cgtacaaata acccctgtat cctttgcggt
    49801 acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    49861 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
    49921 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
    49981 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
    50041 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
    50101 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
    50161 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt
    50221 ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt
    50281 tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat ttcggaaaat
    50341 cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag atcctaccat
    50401 gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt catcggacat
    50461 ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt gattcgatgc
    50521 gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag gtgaagggtc
    50581 aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc
    50641 taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt taaaagtaaa
    50701 tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg aaatgtaacc
    50761 atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa
    50821 tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt taggcaatcc
    50881 gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt ctcgattcaa
    50941 ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga cagggtcttc
    51001 ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt gtttttcttc
    51061 tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca cgacagtcca
    51121 actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc cattcgctga
    51181 ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat cggcagaaac
    51241 aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga ttttagattg
    51301 tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt ggatctggtc
    51361 gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt gtgtgatcaa
    51421 tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt
    51481 ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat taaaatcaga
    51541 taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat gataccattt
    51601 aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt ttaaggttaa
    51661 gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca aaaactaatt
    51721 ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa
    51781 attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac agattttggc
    51841 agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga gaaacatcgc
    51901 gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata tggatgactt
    51961 gagacaagct ctttttgagc aaatctatga tcatttacaa tatgaagtgt tcactgttga
    52021 gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg caagaagaga
    52081 gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag agatgcagaa
    52141 tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg tgaatctttc
    52201 agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg ggatcgcagc
    52261 tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga tgatccaaga
    52321 cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa tatactcaaa
    52381 agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct
    52441 tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg aataaaagag
    52501 ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa atttttgcga
    52561 ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca ttcagatcca
    52621 ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat
    52681 ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt
    52741 ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca tcaggcagat
    52801 cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg aattcatctg
    52861 catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca aatgcttctg
    52921 caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg atttttgcta
    52981 aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat accaaaacat
    53041 cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct aaatgtatgc
    53101 cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt actttaaaat
    53161 cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat gtacataaaa
    53221 atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta tttgtcagaa
    53281 actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg
    53341 cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa aagcctttga
    53401 aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc atttctgatt
    53461 cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg atggaagtaa
    53521 cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg attggtgaca
    53581 tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta
    53641 ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat tacggagaag
    53701 ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct atggttaata
    53761 ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca agagacggcg
    53821 gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca agaattttga
    53881 catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg ggagaggtac
    53941 atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat tgagacgact
    54001 caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc ttattattta
    54061 ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt tctccacatc
    54121 gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt ttaccagttt
    54181 atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt atccgtttct
    54241 gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat
    54301 aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt ctcactaatt
    54361 gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg gttgggacag
    54421 ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat cgatcagttc
    54481 caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt cttgatttat
    54541 ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac gatcttcgct
    54601 actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta ttttgcaacg
    54661 aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg gcttaatttt
    54721 gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga atacgtgacg
    54781 ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa attgaaaact
    54841 aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta tatcagtaat
    54901 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    54961 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    55021 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    55081 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    55141 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    55201 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    55261 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    55321 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    55381 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    55441 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    55501 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    55561 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    55621 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    55681 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    55741 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    55801 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    55861 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    55921 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    55981 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    56041 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    56101 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    56161 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    56221 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    56281 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    56341 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    56401 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    56461 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    56521 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    56581 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    56641 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    56701 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    56761 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    56821 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    56881 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    56941 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    57001 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    57061 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    57121 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    57181 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    57241 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    57301 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    57361 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    57421 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    57481 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    57541 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    57601 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    57661 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    57721 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    57781 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    57841 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    57901 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    57961 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    58021 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    58081 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    58141 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    58201 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    58261 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    58321 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    58381 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    58441 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    58501 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    58561 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    58621 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    58681 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    58741 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    58801 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    58861 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    58921 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    58981 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    59041 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    59101 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    59161 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    59221 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    59281 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    59341 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    59401 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    59461 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    59521 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    59581 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    59641 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    59701 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    59761 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    59821 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    59881 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    59941 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    60001 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    60061 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    60121 ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    60181 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    60241 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    60301 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    60361 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    60421 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    60481 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    60541 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgagta
    60601 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    60661 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    60721 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    60781 aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca gggcacgatt
    60841 tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag cctgagttta
    60901 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    60961 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    61021 gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    61081 taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg aaatggacaa
    61141 ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt ttcaacagat
    61201 gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac agaattaaaa
    61261 cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga agaagaagca
    61321 aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact tgcagcaaaa
    61381 gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    61441 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    61501 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    61561 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    61621 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    61681 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    61741 aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga tacagatgta
    61801 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    61861 gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg gcaagaagca
    61921 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt ttatcaaata
    61981 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    62041 agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    62101 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    62161 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    62221 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    62281 tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    62341 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    62401 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    62461 ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag ctctatcgct
    62521 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    62581 gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt atttctacaa
    62641 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    62701 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    62761 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    62821 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    62881 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    62941 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    63001 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    63061 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    63121 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    63181 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    63241 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    63301 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    63361 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    63421 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    63481 ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    63541 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    63601 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    63661 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    63721 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    63781 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    63841 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    63901 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    63961 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    64021 cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg agagtgaaga
    64081 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    64141 tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca actttgttta
    64201 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    64261 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    64321 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    64381 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    64441 tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    64501 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    64561 tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    64621 ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc gttgcagcct
    64681 ttttaggtat ttgggtctgc cgatctagag gtgcattagt cgcgttgatt gtttttgcga
    64741 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcctaca
    64801 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    64861 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    64921 ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac caatttttag
    64981 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    65041 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    65101 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    65161 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    65221 gatatgatga accagaagtt acggaggaat tctatgttat ggaagaggca tatgacgata
    65281 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    65341 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    65401 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    65461 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    65521 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    65581 tttatttatg aaggacattt atcagaacag aaaattactg ctacagtttt cgatcaatga
    65641 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    65701 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    65761 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    65821 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    65881 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccattt
    65941 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    66001 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    66061 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    66121 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    66181 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    66241 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    66301 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    66361 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    66421 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    66481 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    66541 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    66601 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    66661 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    66721 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    66781 tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa cctattcaag tgggatgttc
    66841 gttcgtctag cttttgctgt agcgatcaat gtgaatcctg atattttgat tgtggatgaa
    66901 gcattggcag taggagatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    66961 aagaactcgg gaaagacgat tttattcgtt tctcatgata ttagttcgat tcgccgtttt
    67021 tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg aatatggtga tacgatggaa
    67081 gtaacgacga attacgaaaa ctttttgaag aaaaaatcag ttaaaacagt agatcgaaat
    67141 aaaaaccaag tagaacgctt cgcagctcca gatatcgtag acgtgacgaa agccgaattg
    67201 ttggataaag ataaaaatcc agtggatatg ctggaacaag gagaaaaact atacgttaaa
    67261 gtaacctatg aagtaaaaga cgatacaatc aaaaatccag ttttaggaat tgctctgcgt
    67321 acagtcgaca atttttatgt gtgcggactc aatactctac tagatggtat caagatccct
    67381 tggaaaaaag gtgagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    67441 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatacaag
    67501 acaaaataca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    67561 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    67621 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    67681 gtttggtcct ggaaacggcc gattaacgaa acatttgatc ggagcaaaac aatgtgaagt
    67741 gagcattgtc gagctagata aggaattgtt cgattttgtc agcgagtttg cccaagatgg
    67801 attttatgga gatatcgaaa gttttgaatg ggcaaactat tatgctggac aaacatttga
    67861 ttacgttttg tttgccgatg ttttagaaca tttggtagat cctggaaaaa cgttgaagaa
    67921 agttagagaa tttttgaatg aagaaggaga aattctaatt acattcccta accttgcgca
    67981 taattctgtc atgattgatt tgttcaataa tcaattgcca tgggcttctt atggtttgct
    68041 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    68101 aggattatac atcaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    68161 atcaacttat gaagaattgc cggaagctgt acgttatgat ttcaaaatgc gaccatttgg
    68221 cgaagtctac caatacttct tctcattgat gaaacatcca gtagcacaaa gttccattgc
    68281 gcagccgcaa aattctaatt atgtgaaagt attagaagta atccagcaaa cgaaacaaga
    68341 tgaaataatt caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    68401 ccatgtatca gaattagcag aacgaatggt gtttcgcttc gcgcagcaac ctagctttat
    68461 cgaattcagc gcagaagctg ctggagaaaa actcacattt atcgattcaa atgctgtagt
    68521 caaaacagta aatgattgtt atttgtttga tggtaaagat ttaccagagt tctttttaac
    68581 ggatattgca gggaaagaag tgactatcca ttgtcactat cgttttatcg gagaattgac
    68641 accaacaatg aaagaattgc tggaaacaat ccgtccaatg gctgaagtta caaagcagct
    68701 ttctgagaaa aatgatgaat tagaaagaga aaatcattat cttattgaga ataatacaag
    68761 attagatcag acgttgaaaa caaccataaa tcgctattgc acgttgttgg aaagtgatga
    68821 atggacaatc aaacatagat tagggcgacg taagaaagaa acatcgaaaa aaatccaaga
    68881 aaaagagctt tctctatgta ttgacgaaaa aatctgggat gcagaaacaa agatattgaa
    68941 aatcatcggg tggggaatcg ctaattcgga tcgccaacca ttgtcctata agttatcagc
    69001 cgaccaatca ccattttttg aagcaattcc tgttttacga gaagaagtca atcaagcaaa
    69061 acagctagct gaaggagcaa aagcaggatt tgaactgcgg atcctttgcg agaaggaacg
    69121 ttcattctta gttgaagcag tggctcaaaa tggtcaatca tggatcattg aaatgtaaat
    69181 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    69241 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    69301 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    69361 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    69421 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagcaat cttagacttc
    69481 caaacagcaa gacatcaaaa tggcatagct gtgaaattaa acggtaaata tccttatgat
    69541 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    69601 gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca aactggaatt gaaaccagga
    69661 tcgatcgact atgcagagtg gatcagtcgt catgagaaac ctgatctgaa agagcaaagg
    69721 aaagaagtac aaggatttgc ctaccgccca ttgatttcca tacttatgcc tgtctataac
    69781 gttgaaatca aatggctcga aaaatgcatt gattcggtac ttgatcaaac gtatgatcac
    69841 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctagaa
    69901 tcctatcaag caaaagatga tagaattaaa gtcgttttcc gacaagaaaa tggacacatc
    69961 agcctggcaa ctaattctgc attggaaatg gcagaaggag agtttatagc gctcttagat
    70021 aatgatgacg aattacctcc atttgcctta tatgaagtgg caaaagtgct aaacgtacat
    70081 ccagaactgg atttgatcta cagtgacgaa gataaaatcg atgcagatgg gaatcgcttt
    70141 gacccgcact tcaaagcaga ctggtctccc gatacattga tgggaaataa ttatatctct
    70201 cacttaggcg tatatcggac aagcattgtt aaggaacttg gcggtttccg taaaggatat
    70261 gaaggctctc aagattatga tttggttctt cgtgtgacag aacaaattcc tgcagaacac
    70321 atttaccaca ttgaccgtgt cttgtatcat tggcggacga tcccgggatc aactgcaagc
    70381 aatggagaag ctaaaagcta catctatgat tctggagtga aagcattgac agatgctcta
    70441 tctagaagaa atatcaaggg aagcgtacat cctggcagaa tttctggttt ttatgaaatc
    70501 acttatgatg ttttgcaaga agacttggtc agtgtgatca ttccaactaa gaatggctat
    70561 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttatcc aaattatgaa
    70621 atcattattg cagataatgg aagtacagat cctaaaatgc aagaattatt tgcagaatac
    70681 aagcatcagc tgaaagaccg atttatcgtc gaattaattg acattccatt caattattca
    70741 cgtatcaaca atctggcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    70801 gatacagaag tgattgaacc tgactggatg acagcgatgg tttcttatgc acagtttgat
    70861 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    70921 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    70981 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    71041 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    71101 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    71161 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    71221 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    71281 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    71341 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    71401 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    71461 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    71521 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    71581 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    71641 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    71701 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    71761 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    71821 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    71881 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    71941 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    72001 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    72061 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    72121 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    72181 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    72241 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    72301 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    72361 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    72421 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    72481 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    72541 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    72601 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    72661 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    72721 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    72781 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    72841 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    72901 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gt
//
LOCUS       DACDEA010000005        72660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDEA010000005 DACDEA010000000
VERSION     DACDEA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.005"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IV136_000345"
     CDS             100..570
                     /locus_tag="IV136_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8464364.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IV136_000346"
     CDS             792..1895
                     /locus_tag="IV136_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8464365.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IV136_000347"
     CDS             2116..3075
                     /locus_tag="IV136_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8464366.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3245..3925
                     /locus_tag="IV136_000348"
     CDS             3245..3925
                     /locus_tag="IV136_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8464367.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4015..5049
                     /locus_tag="IV136_000349"
     CDS             4015..5049
                     /locus_tag="IV136_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8464368.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5063..5398
                     /locus_tag="IV136_000350"
     CDS             5063..5398
                     /locus_tag="IV136_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464369.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5382..7862
                     /locus_tag="IV136_000351"
     CDS             5382..7862
                     /locus_tag="IV136_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464370.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7918..9477
                     /locus_tag="IV136_000352"
     CDS             7918..9477
                     /locus_tag="IV136_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8464371.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9474..10838
                     /locus_tag="IV136_000353"
     CDS             9474..10838
                     /locus_tag="IV136_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8464372.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11009..12079
                     /locus_tag="IV136_000354"
     CDS             11009..12079
                     /locus_tag="IV136_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464373.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12094..12762
                     /locus_tag="IV136_000355"
     CDS             12094..12762
                     /locus_tag="IV136_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464374.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12782..13360
                     /locus_tag="IV136_000356"
     CDS             12782..13360
                     /locus_tag="IV136_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8464375.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13761..14672
                     /locus_tag="IV136_000357"
     CDS             13761..14672
                     /locus_tag="IV136_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8464376.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15050..15967)
                     /locus_tag="IV136_000358"
     CDS             complement(15050..15967)
                     /locus_tag="IV136_000358"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8464377.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16169..16606
                     /gene="zapA"
                     /locus_tag="IV136_000359"
     CDS             16169..16606
                     /gene="zapA"
                     /locus_tag="IV136_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8464378.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16664..17212
                     /locus_tag="IV136_000360"
     CDS             16664..17212
                     /locus_tag="IV136_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8464379.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17327..19687
                     /locus_tag="IV136_000361"
     CDS             17327..19687
                     /locus_tag="IV136_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8464380.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19774..20088
                     /gene="trxA"
                     /locus_tag="IV136_000362"
     CDS             19774..20088
                     /gene="trxA"
                     /locus_tag="IV136_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8464381.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20270..22048
                     /gene="uvrC"
                     /locus_tag="IV136_000363"
     CDS             20270..22048
                     /gene="uvrC"
                     /locus_tag="IV136_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8464382.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22193..23092
                     /locus_tag="IV136_000364"
     CDS             22193..23092
                     /locus_tag="IV136_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464383.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23085..24347
                     /locus_tag="IV136_000365"
     CDS             23085..24347
                     /locus_tag="IV136_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464384.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24508..26406
                     /locus_tag="IV136_000366"
     CDS             24508..26406
                     /locus_tag="IV136_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8464385.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26736..27455)
                     /locus_tag="IV136_000367"
     CDS             complement(26736..27455)
                     /locus_tag="IV136_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8464386.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27792..28124
                     /locus_tag="IV136_000368"
     CDS             27792..28124
                     /locus_tag="IV136_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464387.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28111..30114
                     /locus_tag="IV136_000369"
     CDS             28111..30114
                     /locus_tag="IV136_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8464388.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30133..30603
                     /locus_tag="IV136_000370"
     CDS             30133..30603
                     /locus_tag="IV136_000370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8464389.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30624..31208
                     /locus_tag="IV136_000371"
     CDS             30624..31208
                     /locus_tag="IV136_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8464390.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31223..32209
                     /locus_tag="IV136_000372"
     CDS             31223..32209
                     /locus_tag="IV136_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8464391.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32199..32510
                     /locus_tag="IV136_000373"
     CDS             32199..32510
                     /locus_tag="IV136_000373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8464392.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32532..34313
                     /locus_tag="IV136_000374"
     CDS             32532..34313
                     /locus_tag="IV136_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8464393.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34306..35682
                     /locus_tag="IV136_000375"
     CDS             34306..35682
                     /locus_tag="IV136_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8464394.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35689..36324
                     /locus_tag="IV136_000376"
     CDS             35689..36324
                     /locus_tag="IV136_000376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8464395.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36354..37709
                     /locus_tag="IV136_000377"
     CDS             36354..37709
                     /locus_tag="IV136_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8464396.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37901..39295)
                     /locus_tag="IV136_000378"
     CDS             complement(37901..39295)
                     /locus_tag="IV136_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8464397.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39387..40358
                     /locus_tag="IV136_000379"
     CDS             39387..40358
                     /locus_tag="IV136_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8464398.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40355..41137
                     /locus_tag="IV136_000380"
     CDS             40355..41137
                     /locus_tag="IV136_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8464399.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41306..41677)
                     /locus_tag="IV136_000381"
     CDS             complement(41306..41677)
                     /locus_tag="IV136_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8464400.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41811..42254
                     /locus_tag="IV136_000382"
     CDS             41811..42254
                     /locus_tag="IV136_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8464401.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IV136_000383"
     CDS             complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IV136_000383"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8464402.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43125..44492)
                     /locus_tag="IV136_000384"
     CDS             complement(43125..44492)
                     /locus_tag="IV136_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8464403.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44476..45306)
                     /locus_tag="IV136_000385"
     CDS             complement(44476..45306)
                     /locus_tag="IV136_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8464404.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45469..45906
                     /locus_tag="IV136_000386"
     CDS             45469..45906
                     /locus_tag="IV136_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8464405.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45982..46602
                     /locus_tag="IV136_000387"
     CDS             45982..46602
                     /locus_tag="IV136_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8464406.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46791..47297
                     /locus_tag="IV136_000388"
     CDS             46791..47297
                     /locus_tag="IV136_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8464407.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47314..48213
                     /locus_tag="IV136_000389"
     CDS             47314..48213
                     /locus_tag="IV136_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8464408.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48300..49184
                     /locus_tag="IV136_000390"
     CDS             48300..49184
                     /locus_tag="IV136_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8464409.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(49376..49447)
                     /locus_tag="IV136_000391"
     tRNA            complement(49376..49447)
                     /locus_tag="IV136_000391"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49412..49414),aa:Arg,seq:ccg)
     gene            49507..50841
                     /gene="rpoN"
                     /locus_tag="IV136_000392"
     CDS             49507..50841
                     /gene="rpoN"
                     /locus_tag="IV136_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8464410.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            51044..52081
                     /locus_tag="IV136_000393"
     CDS             51044..52081
                     /locus_tag="IV136_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8464411.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            52122..53123
                     /gene="gap"
                     /locus_tag="IV136_000394"
     CDS             52122..53123
                     /gene="gap"
                     /locus_tag="IV136_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8464412.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            53233..54423
                     /locus_tag="IV136_000395"
     CDS             53233..54423
                     /locus_tag="IV136_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8464413.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            54527..55282
                     /locus_tag="IV136_000396"
     CDS             54527..55282
                     /locus_tag="IV136_000396"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8464414.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            55398..56696
                     /gene="eno"
                     /locus_tag="IV136_000397"
     CDS             55398..56696
                     /gene="eno"
                     /locus_tag="IV136_000397"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8464415.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            57096..58235
                     /locus_tag="IV136_000398"
     CDS             57096..58235
                     /locus_tag="IV136_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8464416.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(58302..58877)
                     /locus_tag="IV136_000399"
     CDS             complement(58302..58877)
                     /locus_tag="IV136_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8464417.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(58882..59856)
                     /locus_tag="IV136_000400"
     CDS             complement(58882..59856)
                     /locus_tag="IV136_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8464418.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(59880..59924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            60139..61107
                     /locus_tag="IV136_000401"
     CDS             60139..61107
                     /locus_tag="IV136_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8464419.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            61220..61498
                     /locus_tag="IV136_000402"
     CDS             61220..61498
                     /locus_tag="IV136_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8464420.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            61616..61852
                     /gene="secG"
                     /locus_tag="IV136_000403"
     CDS             61616..61852
                     /gene="secG"
                     /locus_tag="IV136_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8464421.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            61964..62710
                     /locus_tag="IV136_000404"
     CDS             61964..62710
                     /locus_tag="IV136_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8464422.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            62713..65070
                     /gene="rnr"
                     /locus_tag="IV136_000405"
     CDS             62713..65070
                     /gene="rnr"
                     /locus_tag="IV136_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8464423.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            65092..65556
                     /gene="smpB"
                     /locus_tag="IV136_000406"
     CDS             65092..65556
                     /gene="smpB"
                     /locus_tag="IV136_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8464424.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            65858..66577
                     /gene="atpB"
                     /locus_tag="IV136_000407"
     CDS             65858..66577
                     /gene="atpB"
                     /locus_tag="IV136_000407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8464425.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            66630..66845
                     /gene="atpE"
                     /locus_tag="IV136_000408"
     CDS             66630..66845
                     /gene="atpE"
                     /locus_tag="IV136_000408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8464426.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            66936..67460
                     /gene="atpF"
                     /locus_tag="IV136_000409"
     CDS             66936..67460
                     /gene="atpF"
                     /locus_tag="IV136_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8464427.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            67447..67989
                     /locus_tag="IV136_000410"
     CDS             67447..67989
                     /locus_tag="IV136_000410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8464428.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            68012..69568
                     /locus_tag="IV136_000411"
     CDS             68012..69568
                     /locus_tag="IV136_000411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8464429.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            69582..70484
                     /locus_tag="IV136_000412"
     CDS             69582..70484
                     /locus_tag="IV136_000412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8464430.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            70507..71913
                     /gene="atpD"
                     /locus_tag="IV136_000413"
     CDS             70507..71913
                     /gene="atpD"
                     /locus_tag="IV136_000413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8464431.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            71927..72349
                     /locus_tag="IV136_000414"
     CDS             71927..72349
                     /locus_tag="IV136_000414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8464432.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        24161 a        12722 c        15430 g        20347 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa gaatattcat gtaaacgatt
     3121 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
     3181 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
     3241 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
     3301 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
     3361 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
     3421 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
     3481 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
     3541 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
     3601 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
     3661 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
     3721 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
     3781 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
     3841 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
     3901 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
     3961 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
     4021 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
     4081 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
     4141 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
     4201 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
     4261 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
     4321 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
     4381 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
     4441 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
     4501 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
     4561 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
     4621 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
     4681 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
     4741 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
     4801 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
     4861 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
     4921 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
     4981 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
     5041 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
     5101 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
     5161 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
     5221 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
     5281 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
     5341 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
     5401 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
     5461 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
     5521 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
     5581 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
     5641 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
     5701 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
     5761 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
     5821 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
     5881 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
     5941 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
     6001 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
     6061 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
     6121 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
     6181 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
     6241 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
     6301 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
     6361 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
     6421 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
     6481 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
     6541 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
     6601 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
     6661 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
     6721 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
     6781 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
     6841 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
     6901 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
     6961 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
     7021 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
     7081 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
     7141 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
     7201 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
     7261 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
     7321 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
     7381 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
     7441 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
     7501 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
     7561 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
     7621 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
     7681 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
     7741 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
     7801 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
     7861 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
     7921 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
     7981 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
     8041 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
     8101 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
     8161 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
     8221 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
     8281 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
     8341 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
     8401 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
     8461 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
     8521 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
     8581 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
     8641 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
     8701 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
     8761 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
     8821 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
     8881 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
     8941 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
     9001 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
     9061 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
     9121 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
     9181 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
     9241 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
     9301 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
     9361 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
     9421 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
     9481 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
     9541 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
     9601 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
     9661 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
     9721 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
     9781 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
     9841 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
     9901 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
     9961 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    10021 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    10081 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    10141 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    10201 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    10261 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    10321 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    10381 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    10441 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    10501 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    10561 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    10621 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    10681 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    10741 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    10801 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    10861 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    10921 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    10981 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    11041 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    11101 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    11161 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    11221 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    11281 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    11341 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    11401 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    11461 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    11521 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    11581 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    11641 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    11701 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    11761 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    11821 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    11881 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    11941 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    12001 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    12061 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    12121 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    12181 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    12241 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    12301 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    12361 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    12421 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    12481 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    12541 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    12601 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    12661 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    12721 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    12781 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    12841 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    12901 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    12961 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    13021 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    13081 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    13141 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    13201 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    13261 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    13321 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    13381 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    13441 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    13501 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    13561 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    13621 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    13681 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    13741 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    13801 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    13861 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    13921 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    13981 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    14041 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    14101 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    14161 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    14221 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    14281 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    14341 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    14401 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    14461 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    14521 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    14581 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    14641 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    14701 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    14761 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    14821 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    14881 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    15001 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    15061 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    15121 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    15181 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    15241 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    15301 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    15361 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    15421 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    15481 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    15541 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    15601 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    15661 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    15721 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    15781 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    15841 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    15901 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    15961 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    16021 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    16081 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    16141 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    16201 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    16261 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    16321 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    16381 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    16441 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    16501 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    16561 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    16621 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    16681 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    16741 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    16801 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    16861 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    16921 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    16981 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    17041 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    17101 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    17161 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    17221 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    17281 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    17341 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    17401 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    17461 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    17521 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    17581 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    17641 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    17701 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    17761 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    17821 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    17881 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    17941 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    18001 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    18061 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    18121 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    18181 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    18241 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    18301 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    18361 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    18421 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    18481 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    18541 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    18601 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    18661 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    18721 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    18781 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    18841 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    18901 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    18961 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    19021 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    19081 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    19141 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    19201 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    19261 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    19321 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    19381 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    19441 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    19501 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    19561 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    19621 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    19681 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    19741 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    19801 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    19861 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    19921 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    19981 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    20041 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    20101 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    20161 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    20221 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    20281 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    20341 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    20401 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    20461 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    20521 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    20581 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    20641 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    20701 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    20761 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    20821 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    20881 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    20941 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    21001 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    21061 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    21121 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    21181 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    21241 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    21301 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    21361 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    21421 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    21481 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    21541 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    21601 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    21661 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    21721 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    21781 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    21841 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    21901 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    21961 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    22021 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    22081 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    22141 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    22201 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    22261 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    22321 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    22381 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    22441 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    22501 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    22561 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    22621 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    22681 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    22741 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    22801 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    22861 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    22921 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    22981 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    23041 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    23101 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    23161 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    23221 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    23281 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    23341 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    23401 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    23461 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    23521 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    23581 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    23641 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    23701 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    23761 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    23821 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    23881 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    23941 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    24001 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    24061 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    24121 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    24181 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    24241 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    24301 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    24361 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    24421 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    24481 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    24541 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    24601 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    24661 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    24721 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    24781 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    24841 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    24901 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    24961 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    25021 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    25081 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    25141 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    25201 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    25261 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    25321 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    25381 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    25441 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    25501 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    25561 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    25621 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    25681 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    25741 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    25801 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    25861 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    25921 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    25981 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    26041 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    26101 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    26161 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    26221 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    26281 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    26341 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    26401 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    26461 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    26521 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    26581 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    26641 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    26701 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    26761 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    26821 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    26881 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    26941 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    27001 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    27061 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    27121 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    27181 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    27241 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    27301 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    27361 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    27421 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    27481 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    27541 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    27601 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    27661 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    27721 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    27781 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    27841 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    27901 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    27961 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    28021 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    28081 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    28141 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    28201 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    28261 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    28321 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    28381 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    28441 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    28501 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    28561 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    28621 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    28681 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    28741 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    28801 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    28861 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    28921 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    28981 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    29041 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    29101 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    29161 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    29221 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    29281 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    29341 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    29401 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    29461 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    29521 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    29581 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    29641 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    29701 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    29761 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    29821 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    29881 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    29941 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    30001 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    30061 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    30121 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    30181 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    30241 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    30301 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    30361 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    30421 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    30481 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    30541 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    30601 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    30661 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    30721 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    30781 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    30841 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    30901 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    30961 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    31021 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    31081 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    31141 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    31201 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    31261 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    31321 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    31381 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    31441 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    31501 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    31561 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    31621 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    31681 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    31741 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    31801 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    31861 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    31921 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    31981 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    32041 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    32101 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    32161 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    32221 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    32281 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    32341 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    32401 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    32461 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    32521 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    32581 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    32641 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    32701 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    32761 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    32821 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    32881 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    32941 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    33001 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    33061 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    33121 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    33181 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    33241 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    33301 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    33361 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    33421 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    33481 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    33541 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    33601 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    33661 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    33721 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    33781 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    33841 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    33901 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    33961 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    34021 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    34081 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    34141 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    34201 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    34261 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    34321 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    34381 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    34441 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    34501 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    34561 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    34621 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    34681 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    34741 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    34801 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    34861 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    34921 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    34981 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    35041 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    35101 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    35161 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    35221 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    35281 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    35341 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    35401 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    35461 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    35521 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    35581 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    35641 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    35701 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    35761 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    35821 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    35881 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    35941 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    36001 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    36061 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    36121 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    36181 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    36241 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    36301 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    36361 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    36421 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    36481 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    36541 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    36601 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    36661 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    36721 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    36781 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    36841 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    36901 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    36961 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    37021 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    37081 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    37141 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    37201 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    37261 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    37321 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    37381 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    37441 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    37501 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    37561 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    37621 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    37681 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    37741 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    37801 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    37861 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    37921 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    37981 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    38041 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    38101 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    38161 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    38221 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    38281 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    38341 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    38401 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    38461 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tcccgtcttt
    38521 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    38581 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    38641 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    38701 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    38761 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    38821 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    38881 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    38941 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    39001 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    39061 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    39121 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    39181 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    39241 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    39301 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    39361 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    39421 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    39481 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    39541 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    39601 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    39661 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    39721 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    39781 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    39841 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    39901 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    39961 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    40021 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    40081 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    40141 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    40201 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    40261 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    40321 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    40381 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    40441 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    40501 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    40561 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    40621 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    40681 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    40741 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    40801 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    40861 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    40921 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    40981 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    41041 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    41101 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    41161 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    41221 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    41281 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    41341 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    41401 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    41461 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    41521 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    41581 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    41641 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    41701 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    41761 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    41821 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    41881 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    41941 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    42001 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    42061 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    42121 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    42181 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    42241 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    42301 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    42361 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    42421 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    42481 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    42541 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    42601 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    42661 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    42721 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    42781 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    42841 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    42901 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    42961 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    43021 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    43081 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    43141 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    43201 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    43261 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    43321 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    43381 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    43441 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    43501 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    43561 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    43621 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    43681 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    43741 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    43801 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    43861 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    43921 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    43981 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    44041 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    44101 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    44161 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    44221 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    44281 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    44341 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    44401 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    44461 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    44521 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    44581 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    44641 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    44701 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    44761 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    44821 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    44881 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    44941 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    45001 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    45061 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    45121 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    45181 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    45241 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    45301 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    45361 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    45421 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    45481 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    45541 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    45601 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    45661 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    45721 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    45781 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    45841 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    45901 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    45961 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    46021 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    46081 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    46141 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    46201 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    46261 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    46321 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    46381 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    46441 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    46501 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    46561 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    46621 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    46681 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    46741 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    46801 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    46861 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    46921 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    46981 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    47041 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    47101 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    47161 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    47221 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    47281 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    47341 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    47401 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    47461 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    47521 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    47581 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    47641 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    47701 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    47761 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    47821 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    47881 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    47941 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    48001 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    48061 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    48121 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    48181 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    48241 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    48301 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    48361 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    48421 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    48481 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    48541 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    48601 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    48661 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    48721 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    48781 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    48841 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    48901 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    48961 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    49021 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    49081 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    49141 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    49201 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    49261 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    49321 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    49381 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    49441 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    49501 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    49561 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    49621 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    49681 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    49741 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    49801 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    49861 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    49921 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    49981 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    50041 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    50101 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    50161 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    50221 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    50281 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    50341 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    50401 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    50461 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    50521 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    50581 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    50641 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    50701 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    50761 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    50821 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    50881 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    50941 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    51001 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    51061 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    51121 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    51181 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    51241 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    51301 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    51361 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    51421 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    51481 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    51541 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    51601 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    51661 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    51721 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    51781 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    51841 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    51901 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    51961 cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    52021 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    52081 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    52141 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    52201 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    52261 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    52321 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    52381 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    52441 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    52501 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    52561 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    52621 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    52681 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    52741 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    52801 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    52861 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    52921 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    52981 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    53041 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    53101 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    53161 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    53221 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    53281 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    53341 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    53401 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    53461 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    53521 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    53581 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    53641 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    53701 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    53761 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    53821 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    53881 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    53941 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    54001 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    54061 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    54121 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    54181 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    54241 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagcc
    54301 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggcggc
    54361 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    54421 taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc
    54481 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    54541 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    54601 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    54661 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    54721 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    54781 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    54841 agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga caccaatcct
    54901 atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    54961 acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc
    55021 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    55081 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    55141 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    55201 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc
    55261 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    55321 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    55381 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    55441 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    55501 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    55561 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    55621 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    55681 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    55741 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    55801 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    55861 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    55921 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    55981 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    56041 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    56101 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    56161 tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    56221 acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta gacgaaaacg
    56281 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
    56341 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
    56401 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
    56461 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
    56521 attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc
    56581 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
    56641 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
    56701 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
    56761 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt tgcaactagc
    56821 ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag
    56881 ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta
    56941 tgagcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag
    57001 tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa
    57061 atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga
    57121 ttttacaaga gatcatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct
    57181 atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg
    57241 gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat
    57301 ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg
    57361 cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct
    57421 tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa
    57481 cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca
    57541 cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca actaattaca
    57601 gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag
    57661 cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta
    57721 cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa
    57781 aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc
    57841 atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg
    57901 cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga
    57961 tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca
    58021 ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag
    58081 ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta
    58141 ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat atgattgaaa
    58201 aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataaa tgaaaaaaca
    58261 gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat ttttcttttt
    58321 gaatatatca agcgttgtgg caacatagtg atgactaatg aaacaacgac gatagacaac
    58381 aaacgatcaa ggtagtctgt acctgcctgt accaagatca cacttgttgt ctgacttaaa
    58441 cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat cccgccaaat
    58501 aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat caaagcgagt
    58561 aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat caatccagca
    58621 agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc tgtagtcact
    58681 ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc aagcaatgca
    58741 ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca taaagcaatc
    58801 gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac gcgagttgcc
    58861 attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt tcataatcgt
    58921 ttcttcttgt gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac gagtatctac
    58981 ttttgtcata attttactgt tagcaggttt ctgccaaaat tcataacgat caaccaatgt
    59041 ttgtccacga cttattcctt gagtttccac agcagtgaag cttttgaaac cttcacagat
    59101 ggccccctcg ataaaataag caacagctaa cggatcattg atgacacatc ctaaaattct
    59161 ttcttgtttc cagtggaagt caaaataaaa acgagtgatc gcttttagat actctccctc
    59221 ttcaggattc atttgacagc agtattcgag gatagtcggt gtcaagacga tttctcttgt
    59281 cacatctaac ccaaccattt ctatcgtttg tttgagattc tcgaatacat aagaagctgc
    59341 ttcaggatca caccagtaat tgtattcagc aactggcgaa cagttaccat ggcttttgta
    59401 agttccaccc atcgagacaa aacgatccat atgtttccca atatttgggt tgactttcaa
    59461 agcaaaagcg atattggtca aaggacccaa agcaatgact gatgtatttg tcttttcttt
    59521 gaaataatca gccaaaaaat caacggcatg gagaggttct gcctgttttc tcaagatcat
    59581 cgggaaattt gtttccccta aaccatccat accatgagtg tcttgtgcac tgatgaaagg
    59641 ttgcttcaaa ggtttttcca ttccttgata aacaggaatg tctaaacgat cacagcgttc
    59701 caggcatttc agcacattct gcgtacctat atctgcaggg acattcccac aaactacggt
    59761 aatagcaact acttcgatat caggggattt gacagctaat gataaagcta aagtgtcgtc
    59821 gatccctgga tcgcaatcaa taattacttt tcgcattatt tccaatctcc tttctttaga
    59881 gatacttagt tttttattct gggaagtttc cgaaccagtc caagacgagc gtcttgatta
    59941 taatgcacga aattagtata ctggctttta ttgcgaatgt aaatgctctc ttacaagtaa
    60001 atctattgag gagaacttta agtaaatgat acaatgaaaa agaaatggaa tttacgtgtg
    60061 ccgaaaataa agaatcctat ggatcggtac attggataat agttagatta tagtttggca
    60121 gaaagcggcg ggtgtacaat ggttattttt tatttcattc ttattctgat aattataggg
    60181 atcagttacc attttctttc ttatttttat gcaaagaaat tagtaagagc gggagtacag
    60241 agaggatatg actggtatga agacactggt catcgtttga tggaccctgc tgcagtcacg
    60301 tcagtagaaa aaaaacgaca aaaattagaa gaagagtgtg aaagcttttg ggagcaggga
    60361 acacccaaaa caatcaaaag ttatgacggg ttgaaactag ctggtcagat gttcatccat
    60421 ccaaataagc agaataaatg ggtcatttgc gtacatgatt accgaagcac agggaaaaga
    60481 gacatgtctc atataggtaa aagatatgca gagaaaggat tcaacgttct tgtacctgat
    60541 ttacgtgcgc acggagaaag tgaaggagaa atcatcggaa tgggatggct agatcggcta
    60601 gatttaattg cctggatcca actcatctta gatgaacagc cgaatgcgtc cattatttta
    60661 cacggcggat caatgggagc ttctacgatc atgatggcaa gtggtgaaaa acttccatca
    60721 gcggtcaaag gatttatttt agacagcgga tatgtttctg tttatgcaga attccgatat
    60781 atgttaagta aaatcactgt ctttcctaaa aaaatggtca tgagatatgc gaatcattat
    60841 gcccagaaat acgccggtta ctccttaaag caagcatcag ctacgagaca gcttggcagc
    60901 aaccatctgc ctttattggt cattcatggt gaaagagatc attttgtccc tacagaagca
    60961 gcgtacacga ttcaaaatgc tacggcaggg gataaagcac tgctgcttgt tccagaagca
    61021 gaacacttgg aagcatcgat gaaagatccg aaaacctatt ggacagtgat tttttcattt
    61081 attgaacaaa gaataacgat gaattaattt tctcttcttt tttgtaagta attcgaggat
    61141 aaataattgt ttatagatca ggagactgct aaaataaggt aggaataaaa aaatatcagt
    61201 tatttgagga gtgcggcata tggaaatcaa agaagaaaaa aatcgtcttg ctttattcaa
    61261 tgatgaacaa caagagatcg gagaaatgac atggtcagat gcgggcccag acatcatgat
    61321 cattgaccat acatttgtag atccagcata tcgtggtcaa aagttagctg aaaagctagt
    61381 gtatactgga gtagaattag caagacgaga cgggaaaaag atcattcctt tatgccctta
    61441 tgcaaaaaaa gagtttgaaa agaaaccaga atatcaagac gtcttacgtc agtcataaga
    61501 ataacagctg aaagttttga actttcagct gttattccat ttatatagag ttgtcacgga
    61561 cttttatcta tgataaagtt aaagagacga tatcttttgg gaaggaggct gcgatatgta
    61621 taatttaatt ttgggaatcg tgatcgtttt atcgattgtt atggtcattg cgattatgat
    61681 gcagccaagc aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg atcaactatt
    61741 cggcaaacaa aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg cagttatggg
    61801 agctatctgg atgatcttat tatttgtcct agcatattta tcttcgaagt agttgaataa
    61861 gcctctcgca aaacggcggg cggctttttt tattgcaaag agaacgctta agaagaagtt
    61921 atgaaagaaa ctatggtaaa atgaggcgaa agaggtgaga cgagtgagac aattaccaaa
    61981 acccctttat gcaaaacatg gaaagcgagc ggttttgctg cttcacgctt actcaggaag
    62041 tccaaacgat gttcgaatgc tcgcacgatt tttggaaaaa gcagattata cagtttatgc
    62101 gccgcttttt aaaggacacg gaacaatgga gccatatgat attttgcaag aaaaagcaga
    62161 aagctggtgg gcagacacta aaaaagccat ccatttttta cagtctgagc agttttctga
    62221 tattgccgta ttaggattat caatgggcgg catctttgct gtacgagcac tagaagaaga
    62281 atcagtgatt ggcggcggtt ttttctgttc gcctttgtct cctgtaaaaa caaacgtgcc
    62341 tgaaaacttt gaaaaatacg tacgtcaagt gctgaagaca gcaggtaaat cagagaaaga
    62401 aatcaatgaa aaagcagttg cttaccgctc gttagctgaa caacaattaa tggatataca
    62461 agatcaggca gcaatcgtag aaagtaggtt gtcagatatc caacagccga tatttttagc
    62521 tcaagctggg aaagatgaaa tgatcgaccc taatggcgta tttgaaactg cacgcaagtt
    62581 gagccgtcag cgagtgacac ttcgatggta tcctgaaagt ggtcatgtga ttacagttgg
    62641 tacagcaaga cgcgaattag aaaaagacgt cttggaattt ttagaaaaat taccttggaa
    62701 tgaggaataa atatgacaaa agaaacgata aaagagaaaa ttctcttttt catggaaagc
    62761 agtcgcaaac gcagtttttc tatggaagaa atcgctgaag gtttagggtt ccaaaaaagc
    62821 gatgatttta aattactagt acaaacgatt gctcaaatgg aaagagaaca gagtatagtt
    62881 ttcaataaaa aaggaaaagt gaaacttcct ttaaaaccaa tcttgatcga aggaacattt
    62941 cgagcaaatg aacgaggatt tggatttgta acgattgatc ccgaagaaga tgacatctat
    63001 atcccaaaag aagcgactgg ttttgcaatg gatggagaca ccgtagcaat cgatatcatg
    63061 aagcgagcaa atacagatat ggatcgcgga gcagagggga aagtagtcga gattcgagac
    63121 cgggcgacta ctcagttagc aggggaattc gttgcttatt cagaagaaga aatacaagaa
    63181 acggatcttt acggcgttgt tatcccgaaa gatagaaagc tcaaccaatt taaagtatat
    63241 gcagcagcag agggagttcg tccagttgac ggaagtattg tgctgatcga actgacccat
    63301 taccctgaaa aagggtatgc gactagttta gaagggataa tcaagcaagt gattggtcat
    63361 aagaatgacc ctggcatgga tatcctctca attgtcgtgg ctaatggtat ccctacgaaa
    63421 tttcctgatg atgtattgtc tgaagcagat gaagttccag atcaaatcag cgaaaaggat
    63481 ataactggac gtagagattt aagaaatcag ctcattgtta cgattgatgg agaagatgca
    63541 aaagatctag atgatgcagt tacggttaaa aaattagata acggtaatta ttttcttgga
    63601 gtacacattg cagatgtttc ttactatgtg acacaaggca gtcaattgga cagagaagca
    63661 tatgaaagag ggacaagtgt atatttgact gatcgagtga ttccgatgat cccgcaacgt
    63721 ttatcgaatg gtatctgttc actgaatccc catgttccaa ggctgaccat gagttgcgaa
    63781 atggagattg atccaaatgg acaagtagtc tctcatgata ttttcccaag tgtgatccaa
    63841 acaacagaac ggatgaccta cacagctgtc aatcaaatct tagaagatca agacgaagaa
    63901 acgatgcaac gctatgctca gctcgtacct atgttccaag aaatgggtga attgcatcga
    63961 atccttgaaa cgatgcgaat caaacgtggc gcgatttctt ttgaggatcg cgaagcaaaa
    64021 atccttgttg atgctgaagg acacccaaat gatatccagc taagaagcag aggaattggt
    64081 gaacgcttga tcgaatcttt catgcttgcg gccaatgaaa cggtagctga acattataat
    64141 aaattaaatc tgccgtttat ttaccgaatc catgaacagc caaaagaaga aaaaatgcag
    64201 cgcttctttg attttgcttc agctttgggc attctggtcc gaggaacgaa aggaacgatc
    64261 acaccaaaag atttacagca tgtgatcgaa ggagtagaag acaaaccaga agctgcagtt
    64321 atcaatacta tgttgctaag aagcatgcaa caagcacgct attcagaaga caattttggg
    64381 cattatggct tagcggcaga ttactacaca cactttacat ctcctattcg acgctatccc
    64441 gatctaatcg tccatcgtct gatccgttct tacagtgaag ataaaagtga aagcaatcaa
    64501 agttcgtggg ctacggaact tcctgaaatt gcagatcata gttctaagat ggaacgtcgt
    64561 gcagtagaaa cagaacgtga agtagattcc atgaagaaag cagaattcat gatggacaaa
    64621 gtgggggaag aattcgatgg gatcatcagc tctgttgtga aattcggcct gtttatcgaa
    64681 ctgccgaata cagtagaggg attgatccat atcaatgaat tgaaacagga ctacttccat
    64741 tttgttgaaa atcatttagc tcttgtaggt gaaagaactg gtctgacttt taaaatcgga
    64801 cagaaagtgc gagtaaaagt agtcaaagct gatccagatg aacgagcagt agattttgaa
    64861 cttgtttccg cagaagaggt tacagcgttg gaacggccaa aaacacgaaa tggaaaatca
    64921 ggaaaacgcg aaggaaaaca agcaggaaaa cgcccagaaa gaaatggcaa gaagaacaaa
    64981 cgatatagcg atagtaagca aacgttcggt aagaaaaaag ggaagaagcc attttacaaa
    65041 ggtgttaaaa agaagaaaaa gaaaaaataa aatgagttgg aggaaacagc tatgccaaaa
    65101 ggcgagggaa aattaattgc acaaaacaag aaagctcgcc atgattattc gatcatcgac
    65161 acgatggaag cagggatggt cttgcaagga accgagatca agtcgatacg aaacagccgg
    65221 atcaatctaa aagatggatt tattcgcgtc cgcaacggag aagctttctt gcataatgtt
    65281 catatcagtc cttatgaaca aggaaatatt tttaatcatg atccgttgcg cacgagaaag
    65341 ttattattac acaaaaaaca aatcatccgg cttgaaaatg aattgaaaaa tactggaatc
    65401 actgttgttc ctttaaaagt ctatattcgt aacggctatg ccaaggtatt gattggtctg
    65461 gcgaaaggga aaaaatctta tgataaacgg gaagatttga aacgaaaaga tatcgatcga
    65521 caaattgatc gaacattaaa aaatttctct agataatttc attatccttt ttcgcatatt
    65581 cggtgctttt gcttttactt aatgataaaa tttgttagca tttaatctga taaaacatga
    65641 ttatcatagg ttttttctta gatcaggagc accgtgtata aagaaaaatt cgcttcgttt
    65701 ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt tttgaatttt ctgtttcatg
    65761 gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc acatgaattg
    65821 attttatgtg aatttcgggg aaaagaggtg aaatggattg gatgaacgct cgctaacgtt
    65881 ccacattgga ccaatttggt ttgatggtac agtttgtata atggtgttac tgacctgttt
    65941 gattgttttc ttcttagctt acttttttac aagaaatctt aagttgaaac caacaggcaa
    66001 acaaaatgcg atagaatggg tcattgattt tagtcgagga atcgtaacag ataatctgcc
    66061 tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca ttgtttttat ttgtttttgt
    66121 tgcaaacaac atcggtttag taacaaaaat cgttttacct aacgaaacaa cactttggaa
    66181 aagcccgaca gcagacccgt ttgttacttt aactttggca tttataatga tcttacttac
    66241 tcacttattt ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt cgttcttgaa
    66301 accatatagt tttatgtttc cgatgaaact gattgaagaa tttacaaact tattaacact
    66361 tgctttacgt ctttatggga atatttatgc gggggaagtt ttgctaacct tgattgcaaa
    66421 aatggtaata agcttaggct ggttctcatt gccgttagca attccattag agatggtctg
    66481 gatcgcattc tccttgttta tcggaagcat ccaagcattt gtcttcgtta ctttatctat
    66541 ggtttatatg agccataaga ttgaagtgga agaataatat tcgttttaaa aacaaactac
    66601 tatttatcta atttaggagg aaaacaatta tgaattatat cgcagcagca atcgcaatca
    66661 tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa acaatcgaat
    66721 caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt atcggggtag
    66781 ctctagtcga agctgttcct atcttagggg tagttgtcgc cttgattcta gtttttggtg
    66841 tttaattgaa tcagtattaa ggcagaaaca atttggtttg ttactgcttt tttacatgaa
    66901 aactagaagt acttcaaaga aaggaaggct cgcctatgct gaatcaattg gcaattgcag
    66961 aagttggcaa tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt ttgatactac
    67021 tggctctctt gaagcatttt gcttggggac caatcagcga tattttgaaa aaacgtgaag
    67081 acaagatcgc caatgatttg gattctgcag aacaatctcg catcaactca gcgaaaatgg
    67141 aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat atcatcaaaa
    67201 atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct caagaagaag
    67261 cagctcgtct aaaaagtaaa gcccaagctg atatcactgt agaacgtgat tcagcgctga
    67321 actctgtaaa agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa atcttgaaca
    67381 aagaattatc tccagaaatg cacgaatcat taatcaatca atatatcgaa ggtctaggtt
    67441 cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc tttatttgaa
    67501 ctggcgatcg attcaaataa agcagaagaa atttatcaag atctcgtaag cttacgtcaa
    67561 atctatgaac aagtacctgg attaggtaat atgttaagtg atgttcgtct agaacctcat
    67621 gaaaaacgag aaatcatgga caagcttgtt agtggctatg atggaatcgt taaaaacttt
    67681 ttagaagttg tttatgcgta taaccgaatg gatgatcttt tgttcatgat cgaagaatat
    67741 gaacgtcgat atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac agcagttcca
    67801 ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat gaactatcaa
    67861 tcagtcgaat taaaacaaat cgttgattct tctattattg gtggcgcgat tgttgaagct
    67921 aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaatatgcg taatcaatta
    67981 aatagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag aaattagtgc
    68041 cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag aaatcggtac
    68101 cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg cgatgagtgg
    68161 tgagttgctt gaattttcaa atggctcata cggaatggcg caaaacttag aatcaaatga
    68221 tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata aagtaaaacg
    68281 aacaggaaag atcatggaag ttccagttgg agaggccttg attggacggg tagttaatcc
    68341 gctaggtcag ccaattgacg gactaggtga aatcgttact gacaaagctc gtccagtaga
    68401 agcaatggca ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc aaacaggtct
    68461 aaaagcaatc gacgctcttg taccaatcgg acgcggccaa cgtgaattag ttatcggtga
    68521 ccgtaaaaca gggaaaactt ccattgcgat cgatacgatc atcaaccaaa aaggtcaaga
    68581 tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta cacaagttga
    68641 aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg gtgcgtctca
    68701 accagcgccg ttgctttata tcgcaccata tgctggtact gcaatgggtg aagagttcat
    68761 gtacaacggg aaacatgtct tgatcatttt tgatgatctt tcaaaacaag ctgttgctta
    68821 tcgtgaactt tccttgctgc ttcgccgtcc accaggtcgt gaagcttatc caggggatgt
    68881 tttctactta cactcacgct tattggaacg tgcagcaaaa ttaagtgatg atttaggtgg
    68941 cggttcaatg actgccttac catttgttga aacacaagct ggagatatct ctgcttatat
    69001 cccaacaaac gttatttcga tcactgatgg acaaatcttc ttagaaagcg acttgttcta
    69061 cgcaggtact cgtccagcgg ttgatgccgg actatctgta tctcgtgtag ggggttctgc
    69121 gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag caagttaccg
    69181 tgaattagaa gcatttacac aattcggttc tgacttggat gcagcaacac aagcaaaact
    69241 aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ttgcatgcac cgctagctgt
    69301 tgaaaaacaa gtagtgattt tgtatgcttt gacacatgga ttcttagata gtatccctgt
    69361 agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca atcattcaga
    69421 tatctttgaa acaatccgta caacaaaaga tcttccagaa gaagaagcgt tgaacagtgc
    69481 gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct ctacagaaga
    69541 taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct tcattaaatg
    69601 aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg gcaatgcaga
    69661 tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt caggtttatg
    69721 caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat gatattgcct
    69781 cagaaaatcc tagaggggat atcaattaca acagcatgtt gctcagccgt cctgttaaga
    69841 aaacgggtta tatcgtcatc actgcagatg gcggtttagt cggtggatat aacagctcga
    69901 tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat gattacgtga
    69961 tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc aatctagcat
    70021 atgagctgcg caatctatct gatcagccaa gctttgatga agtccgcaaa atcgtaaata
    70081 tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt tataaccatc
    70141 atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc tcagacttag
    70201 atccagaaga agcaactact ttcgaacaag aatatatttt tgaaccttca aaagaagaaa
    70261 tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg atcgttgacg
    70321 ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact gataatgctg
    70381 ctaatatcat tgatgagctg acggtttctt acaaccgtgc aagacaaggg gctattaccc
    70441 aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga gattggagga
    70501 aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga cgtggaattt
    70561 tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa aaatgacgaa
    70621 aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt gatccggacc
    70681 attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga tacaggcaaa
    70741 gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt actaggagat
    70801 acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat tcataaaaaa
    70861 gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg gatcaaagtt
    70921 atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg tggtgccggt
    70981 gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga acatggtggt
    71041 atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt gtattatgaa
    71101 atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat gaacgaacca
    71161 ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata tttccgtgat
    71221 gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac tcaagctgga
    71281 tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca accaacattg
    71341 gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg ttcgatcaca
    71401 tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc ggcaacagca
    71461 ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca agggatttat
    71521 ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat cgtgggagaa
    71581 gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg tgaattacaa
    71641 gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt gttagtttct
    71701 cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga acaatttact
    71761 ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg cgaaatcttg
    71821 gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg aatcgaagac
    71881 gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg atcaatattt
    71941 aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag ctatcgtcgt
    72001 tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa tcatcgttcc
    72061 attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg tcgattggat
    72121 cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg ttgctgatag
    72181 tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac aacgcgcaga
    72241 acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac gggcaactgt
    72301 tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa atgagatgcg
    72361 gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg tcagcttcaa
    72421 gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct cagcttattt
    72481 ttcgaagaag ctacttctgg aacaccgtat atcggaggtt gtgaaatcag cgttttgtcc
    72541 tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
    72601 accgagggtc agatgattga acaccgttta tttggaataa gggactgcga caagactttt
//
LOCUS       DACDEA010000006        63515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDEA010000006 DACDEA010000000
VERSION     DACDEA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.006"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..519)
                     /locus_tag="IV136_000415"
     CDS             complement(<1..519)
                     /locus_tag="IV136_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464433.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            730..1176
                     /gene="nrdI"
                     /locus_tag="IV136_000416"
     CDS             730..1176
                     /gene="nrdI"
                     /locus_tag="IV136_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8464434.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1246..3228)
                     /locus_tag="IV136_000417"
     CDS             complement(1246..3228)
                     /locus_tag="IV136_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464435.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IV136_000418"
     CDS             complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IV136_000418"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8464436.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4141..5202)
                     /locus_tag="IV136_000419"
     CDS             complement(4141..5202)
                     /locus_tag="IV136_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8464437.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(5414..5941)
                     /locus_tag="IV136_000420"
     CDS             complement(5414..5941)
                     /locus_tag="IV136_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8464438.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IV136_000421"
     CDS             complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IV136_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8464439.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(7768..8889)
                     /locus_tag="IV136_000422"
     CDS             complement(7768..8889)
                     /locus_tag="IV136_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8464440.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9015..9956
                     /locus_tag="IV136_000423"
     CDS             9015..9956
                     /locus_tag="IV136_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8464441.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(10099..10995)
                     /locus_tag="IV136_000424"
     CDS             complement(10099..10995)
                     /locus_tag="IV136_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8464442.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            11277..12617
                     /locus_tag="IV136_000425"
     CDS             11277..12617
                     /locus_tag="IV136_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464443.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12812..13255
                     /locus_tag="IV136_000426"
     CDS             12812..13255
                     /locus_tag="IV136_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8464444.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IV136_000427"
     CDS             complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IV136_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8464445.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15339..16562)
                     /locus_tag="IV136_000428"
     CDS             complement(15339..16562)
                     /locus_tag="IV136_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8464446.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16594..17433)
                     /locus_tag="IV136_000429"
     CDS             complement(16594..17433)
                     /locus_tag="IV136_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8464447.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17450..18130)
                     /locus_tag="IV136_000430"
     CDS             complement(17450..18130)
                     /locus_tag="IV136_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464448.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(18132..19205)
                     /locus_tag="IV136_000431"
     CDS             complement(18132..19205)
                     /locus_tag="IV136_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464449.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(19855..20526)
                     /locus_tag="IV136_000432"
     CDS             complement(19855..20526)
                     /locus_tag="IV136_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8464450.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IV136_000433"
     CDS             complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IV136_000433"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8464451.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            23952..24389
                     /gene="ndk"
                     /locus_tag="IV136_000434"
     CDS             23952..24389
                     /gene="ndk"
                     /locus_tag="IV136_000434"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8464452.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(24411..25277)
                     /locus_tag="IV136_000435"
     CDS             complement(24411..25277)
                     /locus_tag="IV136_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8464453.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(25511..27589)
                     /locus_tag="IV136_000436"
     CDS             complement(25511..27589)
                     /locus_tag="IV136_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8464454.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            28082..29299
                     /locus_tag="IV136_000437"
     CDS             28082..29299
                     /locus_tag="IV136_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8464455.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(29758..30381)
                     /locus_tag="IV136_000438"
     CDS             complement(29758..30381)
                     /locus_tag="IV136_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464456.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(30537..31934)
                     /locus_tag="IV136_000439"
     CDS             complement(30537..31934)
                     /locus_tag="IV136_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8464457.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(31956..32927)
                     /locus_tag="IV136_000440"
     CDS             complement(31956..32927)
                     /locus_tag="IV136_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8464458.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(32934..35234)
                     /locus_tag="IV136_000441"
     CDS             complement(32934..35234)
                     /locus_tag="IV136_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8464459.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(35236..36561)
                     /locus_tag="IV136_000442"
     CDS             complement(35236..36561)
                     /locus_tag="IV136_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8464460.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            36792..37217
                     /locus_tag="IV136_000443"
     CDS             36792..37217
                     /locus_tag="IV136_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464461.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(37715..38416)
                     /locus_tag="IV136_000444"
     CDS             complement(37715..38416)
                     /locus_tag="IV136_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8464462.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(38432..38872)
                     /locus_tag="IV136_000445"
     CDS             complement(38432..38872)
                     /locus_tag="IV136_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8464463.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(38872..39447)
                     /locus_tag="IV136_000446"
     CDS             complement(38872..39447)
                     /locus_tag="IV136_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8464464.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39858..40364)
                     /locus_tag="IV136_000447"
     CDS             complement(39858..40364)
                     /locus_tag="IV136_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8464465.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(40375..40854)
                     /locus_tag="IV136_000448"
                     /pseudo
     CDS             complement(40375..40854)
                     /locus_tag="IV136_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(41026..41727)
                     /locus_tag="IV136_000449"
     CDS             complement(41026..41727)
                     /locus_tag="IV136_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8464466.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(41835..43277)
                     /locus_tag="IV136_000450"
     CDS             complement(41835..43277)
                     /locus_tag="IV136_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8464467.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(43291..45156)
                     /locus_tag="IV136_000451"
     CDS             complement(43291..45156)
                     /locus_tag="IV136_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464468.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(45277..46128)
                     /locus_tag="IV136_000452"
     CDS             complement(45277..46128)
                     /locus_tag="IV136_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8464469.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(46438..47151)
                     /locus_tag="IV136_000453"
     CDS             complement(46438..47151)
                     /locus_tag="IV136_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8464470.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(47219..47545)
                     /locus_tag="IV136_000454"
     CDS             complement(47219..47545)
                     /locus_tag="IV136_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8464471.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(47668..47895)
                     /locus_tag="IV136_000455"
     CDS             complement(47668..47895)
                     /locus_tag="IV136_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8464472.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(48217..49470)
                     /locus_tag="IV136_000456"
     CDS             complement(48217..49470)
                     /locus_tag="IV136_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8464473.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            49531..50271
                     /locus_tag="IV136_000457"
     CDS             49531..50271
                     /locus_tag="IV136_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464474.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(50357..51115)
                     /locus_tag="IV136_000458"
     CDS             complement(50357..51115)
                     /locus_tag="IV136_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8464475.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(51112..52140)
                     /locus_tag="IV136_000459"
                     /pseudo
     CDS             complement(51112..52140)
                     /locus_tag="IV136_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(52160..55327)
                     /locus_tag="IV136_000460"
     CDS             complement(52160..55327)
                     /locus_tag="IV136_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464476.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(55551..56435)
                     /locus_tag="IV136_000461"
     CDS             complement(55551..56435)
                     /locus_tag="IV136_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8464477.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(56530..56967)
                     /locus_tag="IV136_000462"
     CDS             complement(56530..56967)
                     /locus_tag="IV136_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464478.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(57184..58095)
                     /locus_tag="IV136_000463"
     CDS             complement(57184..58095)
                     /locus_tag="IV136_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8464479.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(58552..58980)
                     /locus_tag="IV136_000464"
     CDS             complement(58552..58980)
                     /locus_tag="IV136_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464480.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(59008..59646)
                     /locus_tag="IV136_000465"
     CDS             complement(59008..59646)
                     /locus_tag="IV136_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464481.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(59650..61686)
                     /locus_tag="IV136_000466"
     CDS             complement(59650..61686)
                     /locus_tag="IV136_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8464482.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            61812..62477
                     /locus_tag="IV136_000467"
     CDS             61812..62477
                     /locus_tag="IV136_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8464483.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        18430 a        13376 c        10768 g        20941 t
ORIGIN      
        1 agctactaca ataaataaaa ataatggcac taatttacaa aatgtgacga tagcattgac
       61 aaagctagca ttttcaactc cacgattcac tagccatgca tatccccata agaagatcgt
      121 actcatcacg atcgatgcca cattttgacc accttcgaat acggggaaga aataacctaa
      181 tgcactcatc aacaatgttg cgaacgccac gtttcctaac cagcatgtga gccagtaacc
      241 ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc
      301 gccttttaaa tctggacgtt ttttattgat attattgatc gataaggaga gcatcaacat
      361 tgctgttcct acgagtaccc agctgaagat agctgggcca ccagccgatc cggagatgtc
      421 cgtcataata ccaaatacgc cacctccaat cgctgaacca atcactaagg cagcaagtgg
      481 aatcaaacca attccttttt tctgttgctc tgtatccatg taagcatcct ccaaagtaaa
      541 accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt
      601 taatagaaat ataattctac cgactttttt ctcattatac aagcaaagcg cctagtccct
      661 ttccaaatta caccaccaaa aaaacttaaa aagatgctat actaaaggga aagaattggg
      721 ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc ttttgccaaa
      781 catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact
      841 ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc
      901 gttcctactt atttagacgg cgggaatggg ttagacaacg gtgacacaga aatcttgacc
      961 gaaacgatgc gcgaatatct ggaatacgaa gacaatcata aactttgcct tggtgtagtc
     1021 ggtagcggta ataaaaactt caacaatcaa tattgtctga cagccaaaca atacgcacaa
     1081 agattcggct ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca
     1141 cgcgtttatc aaattttggc agaaaaaaat aaataacaca aaagaagaag ctgaaaaata
     1201 aatttcggct tcttcttttt atgttctcta ttgtttctat tcttattaag ctttgacatc
     1261 catcacaggt gcttttgctt tcgctttacc tgttttttcc aagacaaagt tttttacacg
     1321 atccatattg gttaccaaga cgatcatgga ggtttctttg tctgctgctt tgattgcttc
     1381 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgtccttctg ttactttcac
     1441 atcgaatggc gtaccgccta agtcaactgt gttgatgccc atgtgaagca agatttctaa
     1501 gccatttggt gtcgtaatgc cgatagcatg ttttgttggg aaaacagaac ttattactcc
     1561 agtaaccggg gaatagatat gtccgttttc tggttctaca aagtaacctt cacccatcat
     1621 tttttgtgca aaaactgggt cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     1681 tgatgataag atagtcacac tttcagctga agcttgaggt actgcttttt caggtacttc
     1741 atttactgtg gctgtttcgg tcgctagttc ttctttttct aaacggtctt taggaatacc
     1801 gaagaagaat gtagcaacga agccaccagc gtaagcagcc aataaaccaa ggatatagcc
     1861 taaccattta ccatcagaga tcaatggaat caaagcggct cctgaaggtc caatagcgat
     1921 tgctccgaca tttccgatca ttccgatcac tgccccaccg atacctccac cgatacaagc
     1981 agtaataaat ggacgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     2041 taagattccg actggtaaag cccctttgat catttctgtt aattctttgt cttttttgca
     2101 gcggatccat aatgccaatg ctgcacccac ttgtcctgct ccggccatcg caaggattgg
     2161 aagcaataag gtacgaccag tttcatcaat catttgtaca tggattggcg tcaagatctg
     2221 atgtagtccg aacatgacca ttggcaagaa taatgtacct agaacgaagc cggcaaatgc
     2281 tcctccaaca ccaagtaccc agttgatccc accgaccatg ccgtcagaaa tgaaccctgc
     2341 taaaggcatg atgaagaaaa tttcgatcag accgatggca atcagggaaa gagttggcgt
     2401 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     2461 taaccaaact gcgaagataa cacctaagat cccgccttgt ccagttgcaa gagcttcacc
     2521 ggtaaacaag tttttcagtg gtgcttctgg attcatccct gtcagcatca caacagcccc
     2581 gataactcca ccaagcgttg gcgttgcacc aaaaacttgt gcagcattga tacctgtata
     2641 gataactaga tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     2701 ccatgttcct gcgtctagtg ttccagctgt gactaagttt gctaaaactg cggcaatccc
     2761 tgccacgatc ccagtgccga caaatgcagg gatcattggt acaaagatat tagagatgtc
     2821 ttttaatacc gatttgattt tagagggttt ttgtttcgat ttttgtgccg ctttcatttc
     2881 ttgtgcccgt tcgtttacac ggtcttttcc agatgcagaa gaacttgctg tagttgcact
     2941 agcagaagga agtttttctc ctaatttcac gcctgctaag tcaaccattt cctgcgctac
     3001 tttgttcact ttcccaggtc cgatgatgac ttggagctgt tcatcctcta ctacgccaag
     3061 tactcctgga atcgctttta attccgagac atttacaaga tcatcattga tgaccttcat
     3121 acgtacacga gtcatgcagt ggacaatgct ttcaacattg cccataccgc ctgctccttg
     3181 ataaatttgt tttgccagtt cttccaaagt caattcgttt tttgccatgg tcttttcccc
     3241 ctcataaggt tccttttaca aaaccgtttg cttcagctaa ttttgcttga gctgcctctt
     3301 tatcactatt tgtcaaaatc atgacgatag ccagtttgac attttgatct gcttcctcaa
     3361 agtacttttc agctgtttcg tagtcagctt ctgtcgcttg catgatgatt cgttttgagc
     3421 gttcaattag tttttcattt gttggtttga cgtcgaccat caagttattg aatactttac
     3481 cgataccgat catactgata gtagaaatca tattcaaaat cagtttttgt gctgttcctg
     3541 acttcaatcg agtagagcct gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat
     3601 ccgcatgctc gctgatttct gcttgtttat tgcaagaaat cgttcctgta acagcaccaa
     3661 tcgattttgc gtaatctaat ccaccaatca catatggtgt acgaccgcta gctgcgatac
     3721 caataactat atcatttttt gttaaattca gcttgattag atcttcttgc cctaattcat
     3781 aagaatcttc ggctccttcg acggctacag tcattgcttc ttctccacca gcaattaggc
     3841 ctatgaccat ttccggttct acaccgaatg tcggtacaca ttcagcagca tctaatacac
     3901 caagtctacc actcgttcca gctcccatat aaatcaagcg accacctttt ttaaaggatt
     3961 caatcgtgtt ggcgatcact ggctcgatac ttggcagttc tttttcaaca gcagcggcca
     4021 ctttctgatc ttctttgttc atgagttgga ctgcttgaga aatactcatt tcatctaatc
     4081 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     4141 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcag
     4201 cggtagttct tctttgatga ctttggctac tacattcact ttttcatccg ctggcaaatc
     4261 atatttagtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     4321 agatcctttc atacgcttag ctgtatttct tactgggatt ggcggaatct ctcgaaaacg
     4381 cgcatctgcg cttcgtatga catctcttgc atcatcttgc cgattcgtat gttttcctaa
     4441 tatatattcg taaaaaactt catctacagg ttccgtatgg agcaagactg tctgttcttt
     4501 ttggaaagaa gcaaattgct cctgaacgtg ttctgacaga cctgcatctc cgatataaac
     4561 aatatccgtg ttacttgcta atagatcaag agcacctgcc agaggatgga tccctcgatg
     4621 ttcttccaaa gttggtaatc cttgatacag tggacctctc aactctgtat ctcctggtac
     4681 aaagccttgt acaaggaatc cttgagtttt gagccattga tttttttcct gataccattc
     4741 tttgtccaac cctgtttcaa gacgtggata ataattatgc caagcttcaa tctgtgagaa
     4801 atcagccgct gcttctttca actcatcgat atcattaggc gtgatcgtgc tggcattcaa
     4861 actgattttc aattgatgtg accagtcggc gatctgctga ttagaaatat gataatccat
     4921 tcttaaccct gttacaccaa ttgctttcaa gggtgttaga tcttctaatg aaaagccagc
     4981 tcgtttcaat gcctctcctg aaatatcgac catcaactct agtttattct ttttcgcttc
     5041 gcttcctagt tcgatcaatc gattttgata agccgaaaca tcatcttccg gtatatgcat
     5101 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     5161 ttctgacaaa gaatcattca taaatattga aaagccaaac atattatccc tctttttctg
     5221 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     5281 tgttaaaata attaaatttt atttctaaaa attggattta taatgccaaa agaattgaca
     5341 cagccacaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa
     5401 cacaaaagtc taattactca taagaaatta attctgtatt gtctaccatg aatgtaaaga
     5461 acccttcatt aggcattgga tgaaaataag cattgatcac tcttatcaag gcattatgac
     5521 agactaacaa gtagacattt tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc
     5581 gtgcgtacac atctaagacc gactctccat ttgggaaacg tacagcaaac gctaaacgcg
     5641 ctttttgaaa gttttcatct ttgcttggca gtccgtcata atccccaaaa tccatttcta
     5701 ccaatcgctc atcagcggtc atcggcaaat tgagacgatc aactactgct tgtgctgtat
     5761 ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta
     5821 ccttttctgc tagtgcctgt gcctgcatct gtcctttttc agtcagcggc acatctgctc
     5881 gcccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca
     5941 tgtgattctc tccttcgttg acaaagttct ccttaaccat accttgttgt ctttgagatt
     6001 acaaggttta gattttctca agagaaataa tctcagtaga tactttatct agaaaggttt
     6061 gatcatgttc cactagtaac atagcaggtt tcacgttcaa aatcagttgt tctaattgtt
     6121 cttgattgaa gacatccaaa taattcaatg gttcatccca tatatagagc tcagctggtt
     6181 gggataaaga tttagccaat tccacttttt tccgttgtcc catactcatc tgttcgattt
     6241 tattatgaaa aacatctctt tccatcccaa gctttcggag attattcaaa aatgcttgat
     6301 agtctactcg gtttttcttc gcgaattccg ccaatgtccc tcgattgtct tcataatttt
     6361 ggcgtacgta actaatggtc aaatgtttgg gctgggattt ttctcctaca accttgccgt
     6421 tgaatgtccc cagaagataa tggataatgg atgactttcc cgcgccgtta ggacctgaaa
     6481 ttgccatccg ctgatgaggt tcgatcgtaa aatgaactgg ctcgaataac agattttcat
     6541 accctaattg aagattttct acgcttaaca gccgtttatg atgagacact tggctattca
     6601 tcgtcaaagc atcgatatac tcgatatctt ttaataattt ttctttctca ctgatctggg
     6661 tttccatccg attcacgatt gctttagatc gtttcatcgt ccgtgcagca tcagcgccta
     6721 ctgctccttt attcactcgc ctagattccg tatcgatgaa tccgactttt ttctttgttt
     6781 tgtccccttc tcgggaacga gaccattctg ctttttcagc tgctgttttc tttagcctgc
     6841 tgacttcttt tttcaatttt tcatttttag ctatttcaaa ttcatcacga agttttttct
     6901 gttcttcata gatagagaaa ttcccttgat aaagttccag ttggctcttt tcgattgcta
     6961 aaacatggtc cactacttca tcgataaatc cccgatcatg actcaccacg atgaatcctt
     7021 gttttttctt tttcaagtaa gccgctactt gttttctgcc agagatatcc aaatgattcg
     7081 ttggttcatc gattaacggg aaatgagtgt catccacaaa taagagtgcc agtaggactt
     7141 ttgtcttctc tcctcctgat agtgtgctga attctctcca taagatttca ggatctgttt
     7201 gcatcagttg gagctctctt tcaatttccc atatctcaaa atccataatc tcatttaaca
     7261 cataataggt caaacgctct ttatcttttg tttgctgcgg gaaatagaca aacgcttgct
     7321 gatggatcac ttgcccttga taagggagtt tgttttgcag gatattcagt aaagttgtct
     7381 ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaag tttagattgg
     7441 cttgttcaaa taataatgtg ccttggctgt catagccgaa tgtcagattt tttatttcga
     7501 ttttagacat tattccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
     7561 aagaggatgc aagggttggg gcacacaaaa agacccatac atcgaaagaa ccaagccaat
     7621 cccgaaaatc tgcacactgc tcccttgtct ccacaaggta cagtctgaat gcagctttca
     7681 tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg
     7741 atagcataaa gaaattgttt ttgtcaacta cttcgggaga atgttgaaaa atctgctgag
     7801 tgtctgtcct aagatttctg ttttttctaa tgtttgcctg tgttccattg cttcctctaa
     7861 atcgattggt cctaattgaa tgcttaagac aatggggaga aaatcaaaga gtccttccag
     7921 ctctcggtct ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac
     7981 tgctacgcca tatggaactt ttccgtggat tgtttgctga tcaatccgcc cttctcctgt
     8041 aatgatgaca tccgcttgtt cgatctgttt ttctaatcca gtgatttggc tgaccaactc
     8101 aaaaccagag accatcttcg ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc
     8161 tccgcttcct gggatgtcat ctaaatccac ctcccaaaca tcttttatct gctgagcaat
     8221 cttttcagct tgtttatcca agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc
     8281 aaattgccaa gcagctcctt gttgacctgt atatgggttg gtcacgtctg cagcgatgat
     8341 cacttttact ttagaaacaa gaacgttcct tcgatccaaa tgaagttttc tagtagataa
     8401 caaaggattt ccttctgtgt acccttctaa agaagcaccc aaagattgga gtaatcctag
     8461 accaccatcg ctacatccgc ttcctcctaa agtgacaatg atttgttcca cttcttgaag
     8521 cagtgtatct ttgataagct ctcccaaggc ataactagaa gcaagtcgga tggtttgatc
     8581 cgttggttta tcaagtaaat ctaatcctaa tacttgtgca gattcaatga ctgcggtatt
     8641 ttttccttgc cagttcgtca ataaataact gatttttcgc tgacgaaata agagatccat
     8701 cccatcaatc gtacgccagg ttcccccgag cgcgtaatag attgcttctg cagtattttc
     8761 tccgccatct gcaatcggca cattgatttt tcttccttta cggtcttccc aaccatttag
     8821 aactgcctga tttaattcaa atgaagaagc acttccttta aatgaatcga ttgccgccaa
     8881 aatatccatc tatatccctc actttcttct taagtaagac actttttttc acttttttca
     8941 aacaaaacaa tgctattttc ttctgttttt ctatatacta aaagggaact cattgaataa
     9001 aggagagaaa aaacatgaaa atcgcaatag ctggagccgg agcaatgggc agccgaatcg
     9061 gattaatgtt gcatcaaagc ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca
     9121 ttgaagccat tcgtacaaat ggattgattg ctgactttaa tggcaaagaa gtagttgcaa
     9181 aactgccaat ctattcacca gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg
     9241 tagccttgac aaaagcgaat caattagatg atatgtttcg ttcgatccaa tccattatta
     9301 ctgagaatac ttatgttttg tgtttactga atggattagg tcatgaagat gtactggaaa
     9361 aatatgtacc aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg
     9421 gtcccggaaa agtcacatta ttaggtgatg gagaaatcga actagagaac ttggaaccag
     9481 aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaaa ttgaatccaa
     9541 tctatagtca taatgttcgt tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa
     9601 atggattatg cacgatattg gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag
     9661 agggcatggt tcgaacgatt gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca
     9721 cattagatca agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg
     9781 ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg
     9841 actacatcaa tggtgccgtt tggcgaaaag gacaaaaata tgatattgct acaccatatt
     9901 gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg
     9961 ctctctgatg agaatcagct ggatcagcag ctggttctca tttttttacc cccatgaaag
    10021 ttcaaaaaga aaaacacaat tctcatgttg ccataagaat tgcgcttgtt tattttcatc
    10081 tatgattggt tggcactttt atttattttt aacagaggaa agatcgtatt catattgttc
    10141 ataaatagcg gagtcagtag tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc
    10201 caacagttct gatgggattg gttgaaatgg cgtatgcaaa aattccggta attcatctaa
    10261 atcacgttct tgaagcaaaa aaatattttc agaatgataa aactgataat gagctatggc
    10321 ttgattatct gtcactagtt tcttttgaaa atacatagat tcaaacgttc gcaaagtcac
    10381 tccttgctgc ccttcccgca acagttctag aatccccctc gattccccgg tttttttcag
    10441 atattcttca taagtaaggt atccaggttg ttcatttcct cgaagcggaa gaataaagta
    10501 attggtcgta acacctcttt ttgtaaattc ctgcttatat ttttctgccc gttcttttct
    10561 gccgttattt gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaatccat
    10621 atgtttcgaa taaaaggaac tattatgttt tagtccaagt tctttcgctt ctaaaaaatc
    10681 aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg
    10741 ttctgcttta aagtggttcc aaaaaaataa cacgattttt gcattggtaa atggacgcac
    10801 gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc
    10861 caactgttgt cgagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac
    10921 atctaaagag cggatggaag gaaaaaaata aagtgattta tttttcgcaa ttagcaatac
    10981 gtcgtttttg ttcatctgtt ccgcctcact ttctctgtca ctttttccta tgatagcctg
    11041 tttaaatagt aaaaagccag taaaacgctt cctattattt ttataaaaat ttaacatttc
    11101 tatgaaaata cgatgaaata aatcattctt ccattaaaat atgcccaaaa gttttctgaa
    11161 tagttgtaat tatctgaaaa tttgttatca ttagtcatta ttattattac tatttattct
    11221 tactatttat tcttactatg cattttgatt gtgaatttaa aagaaagagg ttatttatgg
    11281 aagataaaaa attgaaaaaa caacttagct cccgacatat caccatgcta gctttaggtg
    11341 gagcgattgg tgcaggtcta tttaaaggaa gtggcgaagc gattggaatc gccggccctt
    11401 ctgtattgat cgcttttttg attggcggca taattttata tatcgtgatg aaaggtctgg
    11461 gaaaaattgt tttaagtggt ggtgatacgc accacggatt atctggattg attcgtccat
    11521 atctaggtgc tcatgctgcc gattttaccg attgggtcta ctggtccatg tggattatca
    11581 atatcattgc tgaagctgtt gcagctgctt cctttttaca gttgtggttc ccccacatcc
    11641 cagcttgggt atttgttttt atgttggctg ttttaactac tatcatcaat ctttattcgg
    11701 tacgattatt cgcagagaca gaatattggc tggcctttgc taaaatcagt gtcattatcc
    11761 tattgatcat ttttgctgct tatcttgtcg gtcagcaaat gttgggtact ggtgttttcc
    11821 ctacccttca acaattaact gatcatggtg gttttactcc ccacggtatg aagggaatca
    11881 tcagttcttt gcttgtagtt atctattctt acggcggttc tgagttgatt gctatcacag
    11941 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggc gtcatgggaa
    12001 gaatcatttc cttttacatt attcctttat ttttgctttt gattattttt ccttggaata
    12061 ctttagcagg cacgaacgtt agtccttttg tgatggtatt tgaaaaaatg aatattccat
    12121 ttgcggcaga tatcgtcaat ttcgtgattg ttcttgctct gttctcgtct atcaattcag
    12181 gtgtatatgc ttcttcgaga attttgttct tccgcttgaa agatcggaaa ggttcctcta
    12241 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtgctg ttttgtacca
    12301 gtgttcttta tctaggtgtg gctctttctt attttgtcgg tgacaaatta tttggctatc
    12361 tggcaggttc tctttcctat acagtattat tgatctggat cattatcagt gctgcgtcct
    12421 ttgtactgac actaaggaaa ggaacgttat gggacaaagg aatcagcttt tttgcattag
    12481 cagtcttatg gttgattttc cttggtatcc tattcacgaa ctccattggg gtaactgtat
    12541 tgaccggttt gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgctttcg
    12601 tcttgacgaa tgaatgaaac caatagagaa ttttattcta tttatattga atatctggag
    12661 ctattgaaaa aaaggagctg cgacaaaaat tgttgcagtt cttttttccg aataaacggt
    12721 gttcaaaaac catctgtgca gattaattga ttgttctcca cgctcatgac atgatagaag
    12781 aaaggaatga agaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
    12841 gattggtgtt tcgacggtag tcatgtttgg tatgatgtat ttgaatgttt atgcgcttga
    12901 tcatatcttc ttcagtcgca cacgtgtatt catggcgttg atgatgggtg cagtcatggc
    12961 agtcatcatg ttgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgctatgat
    13021 tttagccgct agtcttcttg tttttgccgg ttccttattt atggtacgaa gccaaacaac
    13081 tgtcaacgat acggcgtgga tgaaggcgat gatccctcat cactctattg ctatcttgac
    13141 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
    13201 ggcacaagaa gaagaaatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
    13261 atccatccaa ataaaaaaag aggatgttcc agaagtgatc aacttcaaga aataagaaga
    13321 agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc atcaagtaat
    13381 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct tattaccgca
    13441 aatgcaagct tttgaacacc gtttattcgt aaaagggagt gtgacaaaag tcttgtcaca
    13501 ctcccttttt acgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt
    13561 tcacaaattt ctttgagatc ccccaacctg taaaggcact gaccatcgaa aacacaggtg
    13621 aaagcaaact gaaaaacacg aatggcaagt aagtgagtgt aggaatgcca aatgtacttg
    13681 ctacgaaact tccggcgatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt
    13741 ctagtgttct ccctaataca gttggatcaa atcctgcttt ttcgtaagtt tctttaaatg
    13801 cattaccagg caggataaca gatagaaatt gttctccaat aaagatattt actccgatgc
    13861 ttgtcatcaa tgttgtaaaa atcaattttg gggtgttcgt catctttctt gatactcgtt
    13921 ccataacggt tgagatcaac ccaaattcga tcaacaaacc acctaatgac aatgttagaa
    13981 taatcaatga cacagttggc atcatgcttt cgattcctcc acggctaagt agtgaatcga
    14041 tttgttggtt tccagtattt gaaacaaatc ccgattcgat gacccctgcc aacttcgtta
    14101 aggaagtttg tgggttttga ataaagatca ttcctactgc tacagcaata ttcaacaaaa
    14161 tcgtcaaaat tgccggtacc tttttccatg cacacaagag cattaaaaat agaggaatca
    14221 gtgaccagat cgaaatagta aagtttgctt ctaaaatatc agtcacttca cgaatagatg
    14281 aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa
    14341 atgccggaac agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa
    14401 tagctgctgc taaatttgtt gattcggata atggagacat cttgtctcca aaaacggcac
    14461 ctgaaacaat ggctccaaca acaagggctg gatttagtcc tagtgttgtc ccaataccga
    14521 aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga
    14581 ttgcacaaac aacaaataca gaagggacga accactggac actaatcaat ttaaatccaa
    14641 tgaccatgag ggttggaata attcctgctt gtatccatac agcaatcaat gaaccgacta
    14701 aaataaaaat aaagattgga atgatccccg gtttgatgcc attgatgatc ccttcgtgca
    14761 gccaatctgt cgggtatttt tttatcactg cgtacaacat gaccattccg atagctgcca
    14821 aaatcgtgat attaggtgaa acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa
    14881 tgaccatcaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt tccatgataa
    14941 tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc
    15001 acaattcatc cgcgattcct cctttataaa aataaaaaga cctgctttca actaaggacg
    15061 aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaacgatta
    15121 ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc
    15181 accgactttc tgaacaaaac acctaaccac tactgcgtga aaagttgact tgtatgaagt
    15241 ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa
    15301 ttatttttat aactttcgcg cacaacttta tctttttact agaaagtagc gccctcttgt
    15361 ttttttcttg ctactatttc ttgatgttct tgtttgattt tttctaatag cttgggttct
    15421 ctcaataaat ctagagctgt attggcaagt aattccgctg caatcgcaat agaagcaagt
    15481 cctttttcac ttttagcagc agctttgaat tcttctgtat gtccggcaat atatgtctca
    15541 gagattgcta cagtaggctg tatcgttgga ataacttggc taacatttcc tacatcagaa
    15601 gaacctagac ttgttctttg ttccgtttct atttcttcat caggaacccc tgcttctttt
    15661 gcatgttgga agaataagtc atcgaaagaa ggcgttacga tgacatcatc gactgcattt
    15721 tggaacaatc caaactcaaa agtcgtaccc gtgcttaatg cagcacctcg tacgatattt
    15781 tccacttttt catatacttt gtctaatgtt tgtcgatttc ctgcacgtaa atagaaacgt
    15841 ccagcagcgt agtctggtat gacattggcc gccacgcctc catctgtaat gattccatgg
    15901 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccgttaaa cacttggatc
    15961 aaggcttcca aagcatttac tcctttttca ggtgctgctg ctgcatgaga agcgacaccg
    16021 taaaatttga tatcgactgg atcatttgct aaagattctg tcgttttccc ataacgaaaa
    16081 gctggatgga cacataatgc tgcatcgaca tcctcaaaaa aaccttcccg aacgaagctt
    16141 cccttagctg atccattttc gcctccttct tcccccggag taccatacac acgaatttct
    16201 ccgccaatct catcaatgaa ttgtttcact atgctggctg ctaatacgga gtaagtacca
    16261 aataaattat gtccgcatgc atgaccaata cctggtaaag catcaaattc tgccaaaaag
    16321 gcaattgtcg gacctggttt attggaaata taacgtgcat caaagcctgt tcggtgccca
    16381 gctacttctt tttctaccca gaacccttct ttttccaatt gtttgattaa agctgctgag
    16441 gaataaaatt cataattact tacttcagga tggtcgtgga tagctagtgc aagtgcttga
    16501 tattctggta aacggtattg aatcagatcc ttcaattgat ttgttgagag tgtggttgtc
    16561 ataaaaattt cctcctatgc tagtcgtttt ttcttatttt ctcccgaatg tttcccacgc
    16621 aggtacgctt gaaccttttg atgttttttc gattactttg gctgtttctt ctgtttgata
    16681 ggcatctact attttttgat acgttttatt gtcttcatct tcttttcttg caacgatgat
    16741 attgacataa ggacgtgcat gctcatcgac tggttctaag aagattgcat cagaagcggg
    16801 attaaatcca gcatctacag ctacaccgct attgatgact gaaatatcta catctgacaa
    16861 agcacgtgct gtttgggaag catctaactc agaaatgtct agattttttt tattctcggt
    16921 aatatcagaa acggtaggcg cttggccttt tgccttgtcc actttgatca atccagcagt
    16981 ttgcaataac agaagagctc tgccgccatt cgtgacatca ttagggattg caacttttgc
    17041 tccatctttg atatcagcca catcttttaa cgtattggaa taaattccca acggtgcatt
    17101 aaccgtattc ccaatcgata ctaaatcagt tccgtgttct tcattgtaat tgtctaaaaa
    17161 gaattgatgt tggaaggaat tcaaatcgat cgatccatct gctaaagccg catttggctg
    17221 attgtaatct gtaaatttca catattccaa atggatgttt tcctttttca aacggtcttt
    17281 cacatcgtcc catacatctg tatcttcccc aatgatccct agttttacgg tcgtttcttt
    17341 tttttcagtt gtagctgctt ttccttggct gcatcctgcc aaccaaataa ttgctacaaa
    17401 tgtaatgact gcaaaacgaa tatttttttt cattttttct cctccatttt caatgtgtga
    17461 cttttttaat cagttgatta ctgataaatt gactcaaaaa aactaagacc agaatcaata
    17521 atgtagcgac aatcgttaca tctgtttgga atcgattata tcctctcgat atagcaagat
    17581 tccctaatcc tccggcacct actgctccag ccatggtagt cagtccgatc acattgatga
    17641 tcgtcaacgc tgagacccga atgataccag gtaacccttc taacaaataa acacgtaaaa
    17701 tgatgccgat tgggctcgtg cccatcgctt ctgctgcttc gatcacccca ggatcaactt
    17761 ctagcagtgc attctcgatt tgacgggcaa aaaacgggac aacgccaaaa atcaaaggga
    17821 ctaaagctgc tttggcacca atcgtcgttc caactaagaa acgagtagtc aaagctaaaa
    17881 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcaactta tcaagtattt
    17941 cgaacagcag cctgttagct agtaatccat ttggacgtgt cactactaac gtcacaccta
    18001 agatgattcc gatgatccca ccaatgaatg ccgtccaaaa agacatatat agtgtttgct
    18061 ttgtcgcttc gatgaattca tccggaatca aatagacatt cggcaaatat gttttaaaaa
    18121 attcagtcat tttagtttcc tccttcgatg actttgatac ggttatctaa ttcatttttt
    18181 tgtaaaatag ttacttttac accagattta atcaaataat ccaatgcttt ttgccgctgt
    18241 ttggtttctc cagctaaagt aacgatcaaa cttcctaagg gtgtttcttg taatagctct
    18301 acattaccgt ataagatatt cgctgttact tggtacttgc tgtaaagatg agcgattaga
    18361 ggttcgttcg tctggttgcc gatataactt aattccacca accatttatt tgcaatctga
    18421 tcggcaaaag cagcatgggc actgattgtt tccaatgctt ggtcaaggtg tgtcgctgtc
    18481 cgaataaaat ctttagtcaa ttcttcttca ggatgactga aaatctggat gctgctacct
    18541 ttttcaatga ttttccccgc ttccataacc gctaccttgt tgcagatttc tttgacaact
    18601 tgcatctcat gagtgatcag gacgattgtt agacctagct gacgatttag tttttttaac
    18661 agtgcaagga tctgaagggt cgttttcgga tcaagtgcac tagttgcttc atcgcataac
    18721 aacactttag gttcatttgc cagagctcgt gcgattgcca ctcgttgttt ttgccccccc
    18781 gacagttgtt taggatacgc agatgctttt tcttccagac cgacaagttc cagtagttca
    18841 ttcacttttt gacgacgttc ctgttttgat tttccagaat actttagaga aaaatctacg
    18901 ttgtcaaaaa tcgttcgact ttccattaga ttgaaatgct gaaagatcat accgatggcc
    18961 tttctttctt ttcgcaattc ttttggagag agctggctta aattcgtctg attgacgatg
    19021 acttctcctg ctgttggttt ctgcaacaaa ttcatcacgc ggactaacgt actctttcct
    19081 gcacctgagt aaccgacgat cccataaaca tccccttttt caatcgttaa atctacagaa
    19141 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaatatc tcgcaattca
    19201 atcattaaca ttcctccttg tctataaaaa aagcatcctc aagaaaatga aaatagtcat
    19261 tttcttgagg acgccttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    19321 ctcctcttca agtaccatca tactctagca agatatcggt cgcagccgtc accgccttac
    19381 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    19441 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    19501 aatattcaaa aaaaaaatcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    19561 cgtggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
    19621 attggcgttc gttttaacgg tcgaacattt ccgaatttgc ctacttatcg acaaattatt
    19681 ctccaaggcc attttcagct tttcttactt gactcttcac accagccaga gcctctctgc
    19741 aaagtttcca agcttacttt ccttctcaac gaaatcaata ttttgttgtt gggaataatc
    19801 tacaggagga aaactgaaaa gtcaagcatg aaatccaaat tattttttct atatttattt
    19861 ttctgttttt ttatctaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    19921 gttcaatttt tccttctctt cgacgctata atttcgaaca aaactggaat cgggaatcaa
    19981 aacaacgcct agtcctgcac gtgaagcggc tgtcgctcct actaaagaat cttccactgc
    20041 aaaagctgaa agagcttcta atcctgttcg ttccaaagct gtcagataag ggactggtga
    20101 aggcttgttc tccgatactt catctccgaa cgtaaacgta tcaaagtaat cggctaattg
    20161 aaagtgatcc aagatatctg ttgctctttt cgtaacagtt gtcgtagcaa tccctagttt
    20221 catatgacgt tcttttaagt ctgtcaatat ttcttttgca tagggtttca attggatagc
    20281 tccacttgtc agctgttgat aaaaatattc ttcacgcttt gctcggactt cctgcacttt
    20341 ttcaggactg cctgctgcct gcactaaaat atcaaaactt tgtttccagt tttttccgcc
    20401 ccaagcgtca acgatttctt tcgggatatg aatatcaaat tgctggaatc cccaaagcca
    20461 accatcccga tacgttcttt ccgtatccac taacaaacca tctaaatcaa atattgcacc
    20521 tttcatctat acattaatcc tttccaaaac aatacaccta gtttagcatg actgatccaa
    20581 aatgaacaga agcatagagc ccagccgcat cacttataag agttcttgga atcgacggat
    20641 atagattttc ttcacagctg tcacaagcgc cagataagcg acgactgtta taaacaacca
    20701 taaccagtaa ttgccaggga gcggcatcaa gcctaactca gcaccaaatc ctgtgaatgg
    20761 aaggactgaa ccgacaatga ttcctaatgt agtgataacc agcatggcaa atgttgcatt
    20821 gctttggata aatggtattt tcggtgtacg caatgcatgg agtactaatg tttgtgtcca
    20881 cagggattcg acaaaccatc ctgcatggaa taatgcgata aaaatcactt gttgtgagct
    20941 atccaatgta tggaaacttc caccaactac agcaggacaa ataacaaaat acatcaacaa
    21001 gtaagtagtg atatcaaaaa tcgaacttgt tggaccaaaa taaaccatga attttccaat
    21061 actagatgca ttccatttct ttggctcatc caaatattct ttatccatcc gatcccatgg
    21121 taaggaaatg caagaaacgt catagatcag attcaaaaat agaagttgca aaggcaacat
    21181 tggtaaaaat ggtaagaacg tactggcaat cagtacggaa aacatattgc caaaattaga
    21241 acttgctgtt gccttgatat atttcatgat gtttccaaaa gtttctcttc cagaaagaag
    21301 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgctg attcttttgc
    21361 gatatccaca gctgtatcca cggaaatacc gatatcagcc actttcattg ctggagcatc
    21421 attgatacca tctcctaaaa atccgacagt atgcccattt ttacgtaaag ctgtcgtcaa
    21481 tcgtgctttt tgctgaggat tcaattttac gaatacttcg tgttcttcag ctacttgcgc
    21541 taattcatga tcattcattt ccgagatttt ctcaccagta atcaattcat tgatttctag
    21601 gcctacttga cggcacacag accgtgtgac taattcattg tccccagtca gaacttttac
    21661 agctacacca tgttctttta aagctttcaa cgcttgtttt gttgtttctt ttggtggatc
    21721 aaggaaagca agataaccaa tcagaaccat gtctgactca tctttgactg aaaattctcc
    21781 aacaacgctt ggattggtct tttgtgcgac accaatcact cgtaagccgt cttcattaag
    21841 tgattgtact tttgcaagca ccgcttcttt tcgttctttt gttaatggtg cgacagtacc
    21901 accaagatca ataaatttag aaatgttcaa catttcttca atcgcgcctt ttgtgatcat
    21961 ctgtgttttg ccagccggat cagcgactac aacactcata cggcgacgtt caaaatcaaa
    22021 aggaatttca tctacttttt gataattgat tgtatctgta tctaaagttt ctgctgcttc
    22081 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tgtttagata
    22141 agcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    22201 gattttgtct tgtgtcaatg ttcccgtttt atccgtacat aacacatcga tcgcaccaaa
    22261 attctggata gcatttaaat ttttgatgac tgttcctttt ttggccatca cacttgctcc
    22321 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggcgttaaac caactgccac
    22381 agataatcca aaaagaaaag cttctaacca atctcctttt gtaagcccat tgataacgat
    22441 aactgctgga gccatcaatg acataaattt gatcagcagc atggatgttt tgtggatgcc
    22501 taattcaaaa cttgattgga tgggtttttc agatagcgtt tctgctacat gcccaaatag
    22561 tgtgtcattt ccgacagaaa caatgattcc ttccgcgctt ccactgatga cattgcttcc
    22621 cataaagaca agattttcat aacttgtttc actttgctga tcgtcttcac atactttcgc
    22681 atgcttttct actggatagc tttcccctgt caatgctgct tgagaaatga atagatcctt
    22741 tgcggacaat aaacgcatat ccgctggtat catatctcct gctgaaagcc gaacaagatc
    22801 tccacagacg atttcttcca taggaatctc tgtatactcg ccatttcgtt tgacagctgc
    22861 cgttactttc acaagtgatt tcagtttttc agctgcttga ttagaacgca cagactggat
    22921 caacgtcatc gtcccactga cgataaccat agtgaagata atggctacac caaagagatc
    22981 tttatctcct ggagcggcta atacgtattc cgtaatgaat gaaataattc ctaatgcaat
    23041 caacactaat gtaaagggtg tgatgtatgc tttcaatatt tctataatga atggtgtttt
    23101 tttgccatat gaaatcgcat tttcaccata agtttctctt gaacgctcga tttgttcttc
    23161 tgacaatcca tttggtgatg catggaacaa ttcaaataat tcttgcttcg tttttccagc
    23221 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcgatct ctcctttgtg
    23281 tccaaaagac agctgttctc gctgtttttg gtcttttgct attcatcgga tcgatagaga
    23341 aaatgctgaa aggggctgag aacagataga taatagccct ggcctttcct ctaaatcctt
    23401 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    23461 tgacttccaa tcatttttcg aatagttatt ttcccaactc gctcacctcc ctttttgttt
    23521 tttcacctat gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt
    23581 tgaaaaagca aatcaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa
    23641 cgtaaaaagc agatatctgc ttttagctac cttatgaaaa gccttatctc gacaattcct
    23701 gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc
    23761 tcacctaaca aatcctattc ggttttccct cgttaatata acaactaaaa aagaggatgt
    23821 caaatcaatt ttccatgttt ttcatacttt tttaaaatac acgcgttata tttcctgtct
    23881 cacgcagttt tatttgtctc attttccacc tcccgctata atagaattat agtaggaaac
    23941 gaggtgctgt tatggaacga acacttgtta ttatcaagcc agacggcgta agacgccatt
    24001 tggtcggaag catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaaat
    24061 ttgcaacgat gacacctgaa ctagcaaaag aacattatca gcacttgact gaacgtagtt
    24121 tttttgatga gcttatcgat tacatgactt ctggtccggt cgtttatctt gtgctggtcg
    24181 gcgaggaagt gatcgatatc gtccgtaaga tggtcggagc aacaaaaggg gcggatgccg
    24241 ttccaggtac gatccgagga gattatgcac tgccggggac tgaaaacatc atccatgctt
    24301 ctgattcccg tgatgctgca acaaaagaga tcgcccgctt tttcccgcca ttcgatacaa
    24361 acaaaccgtc tgaaaaagcg gcgaagtgat acaaatccta aactgttggt ctaactgctg
    24421 ggtttagcaa tggccagcag ttttttctct aaagaattcc tttgattctt ccctaatgta
    24481 tattcgatca ctgcgaaaaa ctcttggtga ttttccgatt tttcttcccc taaagcagca
    24541 gaaatcagtc gttggtaata atccgccatc aatgtttcat aaatcactgt acttctttct
    24601 ttatgcgcta aactccgttt catatcttca ttcacgctac gtgccttttt taagcgacca
    24661 ttttctatat aaaacaaagt caagttcgtc aaaaaagtaa agagatactg ctcgtgatta
    24721 ttcagttgcg cgatccgtcg cattcttttt ttgattttca aataattatt ctcaataaaa
    24781 cgatcactga atatccaagg aaaatctgct agaatagata cttcaaattg cccatactct
    24841 ggtacttgca tcaaatattc ttcaatgata cgacattcat aacgcaaaaa ttcaaattcc
    24901 gtctcagata cttctgtttt tttggacatg tgcagattca aaaccaaata gtacagatct
    24961 tctgttttct tatagtgcct gcggttatta gcaatttctt tgtaaggatc aaagctgtct
    25021 tcttgaatcc gatctagcat tcgtcttttt ttctttaatt tttttggctc atcaagctga
    25081 tttcgtatcc attcatattc ggcaagtgtc atgttcattc gctctaaaaa tgatttcaat
    25141 gttccaaacg atactttttt cactgaattt gtctcgatcc cgactagcga agttttagaa
    25201 cagattcctt ccgctaattg ggtctgtgtc agctttttgt tgactcgaag cttcttaatc
    25261 aattctccat catacataat agttctcctt ttttaattga aattaaccct tggcttttcc
    25321 agaatgcttg ccaataagag gaatgattat ttcaatttaa attttccgca ttcaataaat
    25381 agatacgtta aaaataaaaa atacacgaaa aaaataaaaa aatgtttttc tcataaaaaa
    25441 taccaacctt caagcatgag actgctgtct aatccctgaa ggttggtttg attgtttatt
    25501 gatcattttt ttactgctcc gcattggttc ctgttactgg atataggata gtcgttgttt
    25561 tattgccgtt ttttgaaaag acactggtcg aatgttcacc tttttgcttt tcgattgctt
    25621 cattacgttc cgcttcgttt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    25681 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    25741 tcttgacttt ttcctgatct ttggagattt cctcgtcgat tcctgctgct ttcgtatcga
    25801 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggcgtca
    25861 cgaagttccc atttcgaaaa gcaactactt gatcgtgctg agaagataat aagtctgtac
    25921 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    25981 cgatttctcc accatattca tgattgactg tccctttagt gtaaccaggc atatggatca
    26041 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    26101 caagagcact tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    26161 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    26221 atgattgatc cagataatgg gccgttcgaa cataattatc tactgtgcta ttgccagtat
    26281 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    26341 aaagaaattt cgtgtaaaac ggctgttgta gatgttcaag atattgggca gattctctga
    26401 ataaatcttt atctaatatc ccataaccta acgtttcatc ggattcatca aaataagatc
    26461 gatcaaagaa attgtcatac cctaaatttt tatatacatg atctctattc cagaaactag
    26521 ctacattccc atggaaaaca gcacttgtat agccttctgt ttgaccaagg gtcgctggtg
    26581 ctgcttgaaa gacattatct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    26641 atgtgccagt ttccaacatg ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    26701 agaaattgtc aaaactgatc gttgcttgat tctggaaaat actatttaaa aacggcgtga
    26761 cttcttgtcc gtctaccttc atattgatca aaaactgttg aaaactttct aaatgaagga
    26821 caatcacatt ttttccttta gcaatcccaa atgtttcagg atttggctct gcataatgtt
    26881 tctttgtata gtctaaaact tcatcaattc cattactgct tgcatgcgcg cgaacactgc
    26941 tagtcattcc tgttttgatt ccgtcataaa tagtgtacgc atcgattcct agatatttga
    27001 caatatagga acggtcaaat gtccgctgta gcaattgggg acggttggct tcgctcaacg
    27061 ctaaatttcc tgaaaaaatc aatcctgaaa gacaggtaac agcgatagcc atcggtttgc
    27121 tgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    27181 acacagcgat atccaaccaa tagatgacat catggggttt catcaaagaa aaggagcttc
    27241 ctgacaaccc ttgtgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    27301 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    27361 tcaaaagaat ggctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    27421 ataaggtagt cgcaatcggg ttgatccaca agataaagta ctggatcgta ccttccacac
    27481 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    27541 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    27601 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    27661 gttatcttaa gattttttta ttctttctaa agattattcc attcattttt cacagcataa
    27721 aatcgtttcg gtgtaagcgg ttaaaatagt aaggagataa aaatcttgtg gtgaccgatt
    27781 tactctatag tattcaaaag tagtttctcg gtattcgtac aaaaaaaaca tactaagatt
    27841 agaggggaac atcattgacg aatgatgttc tcctctattt tctattttta gattatagtg
    27901 aagagtgaaa gaaagttcga acaaagcctt gaggactacg agtccctatc aaaaaccgaa
    27961 cttctttact gtcattttag aatcaggttt acgtatgttg gggccatgag cccgtgtata
    28021 ggaaattgga atcgaaatga agcagtaaag ttctttcaac acttaaaaag gaggaaaaca
    28081 aatgccttac attcttgcat tagatgtttc aatgggacac agctacagtg tcctatacaa
    28141 agacgacacc tgcttatttg aagacgagat cgaacacact caaagaggat ttcatgcatt
    28201 actcgaagag atccagcact tgcctgaacg tccattgatt gtttttgaag caacgggtat
    28261 ttattccaga ccagtcgaaa agttctttca agatcatcac ttttcttact gtttgttaaa
    28321 cccgttagaa gcgaaaaaac agatggaaga aaacacctta cgcagttgga aaacagataa
    28381 atcggatgcc catcggctcg cacagactca tcttaaaaat caacgtcaac caaaagaagt
    28441 acaaagcaat ttttacttag agatgcgcga tttagcccgt ttttatcaag aaattgagaa
    28501 gaaaatcact cgactgcgca tggatttaca caactgcctt cagttgactt ttccagagct
    28561 tgaacagttc ttttcaaaca ggctgacccc ttacgcattg acattgatcc gtctttttcc
    28621 ccatccagat tttgtattgg catctactcg gacaaaaata aaaaataaac tgatcaacga
    28681 aacacgggaa aaaatctccg cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta
    28741 tgcacagtgt gcctatccag ctgttgaaaa agacagtatt cactgtcaaa aaacgatcta
    28801 ttacgccgaa cttctccaag atttactcga acaaaaaaaa gcactggcga cacaaatgat
    28861 caaaaaagcc gaagcctctc cttgcttttt gttgtaccaa acgttccctg ggattggcgc
    28921 actcacagcc gctttattgc tcggtgaatt aggagatatc acacgattta aaacccataa
    28981 acaattgaat gcctttgtcg gaatcgacat ccgacggtat cattcaggta agtatactgg
    29041 ccaagaccgc atcaataagc gtggtaatcc caaagcacgg aaaattattt tcttcaccat
    29101 tcgaaatatg attcgccagc aacgcgcagc tcctaatcat attgtcgatt attactacaa
    29161 attaaaaaag caacctatcc ctaagaaaga taaggttgcc acagttgctt gtatgaacaa
    29221 gcttctgaaa tgtatgcacg ctatggttag ggcacataca gagtacgact atgcgtacgc
    29281 ggtctcagtg gaccattaac taaaccactt acagtatacc acttctttgc ccagatcgaa
    29341 aattttttac gaacggggct ttatttagtg tgccttgatt ttgtcgcttt cgtttacgag
    29401 ctatttttct tgcacctttt acttgactaa tcgtaggaaa gaggctgtga caaaattctt
    29461 gtcgcagctc cctatttctt gatgcaggac agctttttca caacctctcc actaaacggt
    29521 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt
    29581 cccgtttttt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    29641 gttccagaag tagcttcttc ggaaataagc cgaaatttta caaaaacttg agaagcactt
    29701 ttcgtaaatt tcttcttatt tcttgaagct gaccacttct gtcacaactt ctgtttttca
    29761 ttgtccatag gtttgatatt gtccttctga tgactcattc gtaatgattg aggcttgatt
    29821 ttcttcattt cccgttatcg tttgtccact gccagaatct gaagggctta gttctgtcga
    29881 ctcgtttttt tgagaagaag cagttgtttt cttgacttgt tcgttggtct ttttccattc
    29941 cgctagtcga tcggccacca cttttttgtc cactttttca gaataagctg taaccacttt
    30001 tccatttttc aaacgtgcta ccttgtcgcc atcatgagtc aaaagcaaaa cgaccaaatg
    30061 tttgtctttg aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattgat
    30121 aaattgattt tcttcttttc cttttggcaa atagatgtcc aattcacctt tttcgtaatt
    30181 ggcagttaat ttatattcct cccaccaata agttgagaat tcttttttga aattatctac
    30241 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    30301 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    30361 aaacggtctt tttttcctca tgccttcctc cactcctatt taattttgac tattctatta
    30421 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    30481 ctttttcctg aagcggtaag aacgggaatt ttcatacatt ctctcttggc tattctttaa
    30541 gcttcaaatc catttctgtc tgatacatct ttgaaccaat gacccgattt cttgatcgtt
    30601 ctttttccat cttgtgctaa atctactgaa ataaaaccgt aacgattctt ataggcattg
    30661 gtccacgacc agttatccat gcatgtccac atatgataac ctaaacaatt ggcaccttct
    30721 tgaatggctt gatggacata tttcagatga gattgaacaa attcgatccg ataatcatcg
    30781 tggatcatac catcttcctt gataaatcgt gcttcatttt ctacacccat gccgttttca
    30841 gaaataaaac agtcgatatt cccataatga tctcgtacat tgatcaaaat atcatagatt
    30901 cctttttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    30961 ttgtcgaagt aatcctcagg cataggaccg tttttagtat cgattggtgt ttcttttgct
    31021 tttattcggc gtggttggta ataattaatt cccaaaagat ctactgtgtt ttctttaatg
    31081 atcgtcaaat catctggatg aatctccggc attagatcga gttcttttat tctctcaatc
    31141 aattcactgg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    31201 tctgctattc gtgctgcttt aacatcttcg ggatttgatt cgtctcttgg ataactcggt
    31261 gtcaaattta agatgattcc gatcacaccg ctttgatcca tttcatggta agctttgatg
    31321 gctaatgctg aagccaacgc ttcatgataa ccaacttgga ttgccttttt catatctgat
    31381 tcttccggat aatgccatcg atacaaatag cctgcctcga ctggaacgat tggttcatta
    31441 tgggtaaacc atttttttac tttcgagcca aacaattgaa aacaactttt tgcgtatttt
    31501 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    31561 atatcgaaat gatacagatt aacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    31621 tagtgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    31681 cggctccatt ggatagaggt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    31741 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    31801 ttttgcggtt cgtttttgta ccaataatca aaaatattct ctccttttcc atcgcctgaa
    31861 attcttcctt ctgtttgtgg accacttgct gcagagcccc accaaaaatt ttttggaaat
    31921 tgatatcttt tcatcaaagc acctcttttt cttttttagt tgattccatg tatgatattt
    31981 tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt caacagctgt
    32041 tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt tggaaggata
    32101 ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg
    32161 acaccttctt gattgaaaca ttcgatggca ccaatcgaca tgttgtaatt gctgacaaaa
    32221 atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa
    32281 tagtctcctg taaagatctc taaatggata gacggatcct tttgtaccgc ttctttggct
    32341 ccagctaaac gttctcttgc tacgtaatct gtttgagcgg cagcaataaa tcctattgtt
    32401 ttatgtcctt gtccgccatg atgagcaatc acttttgcta cactttctcg atcattcact
    32461 actattgtat caatacctgc tggcccaccc ggacaattca atgcgacaac tgggatactc
    32521 atttttgtca gcatttcaaa tccagaccac tcttcttctg ataagaagac gatcaatcca
    32581 tcgatcgcat gcttcatcag gtattcgatt ttttgttcga ttttttctgg ctgatcggca
    32641 aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat ccctgaaaca
    32701 acctccgaac caaatcggtt agatatcccg tttatcaaca gtccaataga gaaacttcga
    32761 ttattcttca atcctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct
    32821 gatatttttt ccatattctt ttccttcagc tgctgaccgt ttaaatagcg ggaaactgtt
    32881 cccactgaaa caccagccat cttagccacg tcacggatcg atgccataca ttttcactcc
    32941 tgccagttac atttgatttc tttttcttgt tgttcgacca tgatgaccgc tttctttgca
    33001 taatgccgct tgtcatcaat atccatataa ctaaagtctt cagaagtgag ttcaaaaaca
    33061 agcagtcttt cctcttgaga ccgcaattca tctttacgaa aagctttcaa taatttcgtt
    33121 ctttggatga caacgccgtc aattaatcgg acatagacaa gagtacttgt tttacctgaa
    33181 acacttcctg tatttttaac tttaattgat attattactt tgtcaatatc cgttttgact
    33241 tccatctgac tgtattcaaa tgttgtatag cttagaccat gtccaaaagg ataaaatgga
    33301 cttccgctta aatcataata attctcttgt ttagaagcag cacgctgata gtaataaaca
    33361 ggtagttgcc gactatttct aggataagac aaactcaatc gtccgcttgg gttatttctt
    33421 ccagaaatca tctgcgcaat ggcttttcca ccctcctgtc caggaaacca tgctgttgca
    33481 atggcattcg taaatgactc gattttcatc aaatcgtagg gtcttccttg cacaagtatc
    33541 gtgatgattg gcttgcctag ttgatacaat tgttcaatca gctgttcttg gtatccacct
    33601 aaagaaagag aagcaacatc tacattttct ccagaatcca tgttgatccc tttcgatgaa
    33661 actgccccgt tacgaagaaa ttccatatca aagttccgcg cacttgatcc acctagaact
    33721 gcaatgatca catctgagtt ttgagcaagg gtcaccgctt ctaataattt ttcttcctga
    33781 tcctcctgat ttcttacttc acatccttgt gcataagaaa cctttgattg gggaaactct
    33841 gtttttatct cttgtactat cttttttttc aaacggtctt ctgtttgtgg tgcagagtag
    33901 tctcccaaca agtggtaaac atcgtgagca tttggaccta taacagcaat gttttttact
    33961 tgttgatcca acggcaatat accgtcattt tttagtaaag tgatactttc ttttgctgtc
    34021 tctaaattta agtgctctga ttcctccatg agatgatccc aatactcctt tggatcgttt
    34081 gcaaatggct gttcaaataa acctaataaa aatttgacag ataaaactcg gcgaactgct
    34141 tgatctagta gcttttcatc tacgacttga ttatcaatgg cttctgcaat cttggtatag
    34201 gtctcatccc acaaactgag atcgattcct gccgctaatg catatgctgc agcagtcttt
    34261 ttatctgtaa aaacatcgga taaacgatcc aaagccacac cgtctgccat aacgattcct
    34321 tgataaccga tctcttttct taatgtttgc tcaaaaagcg ctcgattgat atgacaaggt
    34381 atcccgtcga tgtcattata agcagccatg acacctactg catttcttgt actcttcaat
    34441 aatggtccgt aaatatcgga gaactctctg cgtccaatcg ttactgtgcc tgaattatga
    34501 ccgcctaaag cttctccttg tgcgatacag tgctttaaca caacaccgat ctgatccttc
    34561 ttcttattga tttcctctac tgtctgatca agaaaagaaa ctttttctga aataatctct
    34621 ccttggaacc ctcgaacgac cgcttcagaa aaacgagctg agagaatcgg atcttcccca
    34681 aagcactctt ctgtccttcc ccaacgagga tcttttgcca gatccaaagt cgacaccagc
    34741 gctaaatgaa cacctttagc agccagttct tttgccatca atcgactcga ttgttctaat
    34801 aatgctacat tgaaagtatt tccacggccg atattcgttg gataagaaat cccatccaag
    34861 ccttgatgac catgtggaca ttcttctaca agcaaaactg gaattttcca tcgcgaatgc
    34921 tgtagtacat attcttggac taggttagcc accttccagc tttcttttgc aggaatccca
    34981 ttcttataat ctgttttaga ccacggatca gagcgaaaca gaccgtataa ggcacccaga
    35041 ccgcctcccc atttaacatg ttcttttaac ttttctgtta attcgatctt gtctccgttt
    35101 ttttcataac attcccaacc atacaaatgc tggtttacct gtcctacttt ttcagcaata
    35161 ttcatcctcg aaagtaaatc ttctactctt tggcggatgg ttaactttgg gttttgatac
    35221 gggtaatcgt acaccttaat atcctccttc tgccaatttt gccttattag cggctaatac
    35281 aaatggcagg aataaaaata catctagaat gataagtatt actgttaaga ctgctgctcg
    35341 tatatcgcct cctgtagcaa taaaagattg tataaccggt ggagtgatcc aaggtgcagc
    35401 agcaactgtt ttactgatga ttccaagact tgtggctgcg taggcaataa ccaagttaat
    35461 agatggtata atagcgcatg gaatcatata tattggattc atgactactg gtaatccaaa
    35521 cataattggt tccgtaatat taaataaaca agtagtcaga ccaaatttag cgattgatcg
    35581 ctgttcttga cgtttagaag cccacaagat agcaataact aacggtaaaa tacacccgcc
    35641 acctccgatc aatccaaatg cattggtaaa gggctccgta ataatattag gaatttcttt
    35701 tcctactgaa aaagcatcca tgttttcctg aatagactgt aacaaaggtg cttgccgtat
    35761 aggagccaaa atactcgaac catgcatacc aaacacccat aagaaatcag agaaaaactg
    35821 taaaaataag atgcctggga cggataaaac aaaagctttc aatggtgtct gaatcgctaa
    35881 ttcaattaat tgcggaacat tcaaaccagt cgtatggttt aaagtaaaaa cgacaactga
    35941 aaaaaataaa atgacaacta tttctggtac taaagaatta aatgacgcag ctactgccgg
    36001 tggaactcct tctggcatcc tgattttaaa actcttcatt ttcgctaatt tgataaataa
    36061 ttctgtactg acaagggcag caatcatcgc cgttgctaag ccagacgaag aagtcagact
    36121 ttgtaagaaa acccctgtag cttctgctgt atttccgtcc atcaaagtga ctgtacttga
    36181 taaagacatc aaaataaaca ttacggctgt acttagaatt cctgcatgaa caggactaaa
    36241 tgatggatca tcaatcacgc ctgtttggat atagtgggtt gccaaattcc agccgatatt
    36301 gtaacacacg ataatagcta aaatactcat cgtaccatta ttcacgattg aagcaagttc
    36361 agccaatgga cttagatcaa gtattctttg gactgttgca tttgaaaaaa tcagagcatt
    36421 cagcaaaacc atgacagaag cagccattac taacggcata gtaacgataa aactgtctcg
    36481 aatggccagc aagtgtcgct ggctactcat cttagatacg attggtagca ttttttcaga
    36541 tagtctgtct acgactttca ttatttcatc tcctagattc taaaattgaa ccggttcatt
    36601 tttaatatag cacttttttt aaagattgca acacattttt ttaaaacgct ttctaaaggt
    36661 ggtggatttt ttatgacttt tgaaaaatta ttctatttaa tggtattttg tgaatttttt
    36721 ctcttccatc cgtttgaagt tttactttcc aatggtataa tttctttatc ctatcaaatg
    36781 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    36841 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    36901 ctgtcttaaa agcggaaaaa gcagatatca ccaacagtgt atatacgcta aaagtacgta
    36961 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    37021 gcacgtatca gacacaagga gctgattctt ctgaatcatc tgaagaaaaa acagtgctga
    37081 cagatatcaa gattactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    37141 aagaagcaac agtcacattt accaaaatcg gtaattaccg tttgaaggat aaggatggca
    37201 acatctacag tctatagtgc catctgttgg atcttctttt gtttctcccc ttcttttctg
    37261 ctatagtaaa gaagcaagat gactgtaaag gagacgaaat ggatgatcga tagtcctgat
    37321 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgatca aggaagtttg
    37381 agtatatgcg acaattgcca caaactagtc aaagattctt ttaaatcgtt tgaatcttat
    37441 cctgtcaaaa attatgtaaa aagcaaaaga agataagcaa aatcaagcaa aactcactat
    37501 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc aaaaactgga
    37561 atcagtaaaa caaacagtgg atcatttcag ttcacaaatc aaagagtaac gttttattta
    37621 tttcacatct ttgaatataa aataaccaaa cgatagaaag acttattcta catcacgtag
    37681 taatttttct ttcccgtcgt ttggtttttt acttttagcc taattcttga cctttcgcat
    37741 gctgattatc tgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
    37801 ggatctgacg aatcaccttt tttgcatctt ctggattttc tacaaaatcg aatgtatccc
    37861 catccacttg tattttagga catacatcaa aatcctcata ccattgatta tagcgggtgt
    37921 tcagtgtttc gtaataggtg tataacgttt catcgctttc tagctgttca tagtcacgcc
    37981 cgcgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
    38041 caggacgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
    38101 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcatttaga tggaacaaca
    38161 gactatcttc gtagatggag cggtctagca cattgtcatc gttcactaaa gcatttttca
    38221 ttgctaaaaa tcgcttattc aaaaagaaga tttgcaataa aaatgcgtac tgctcaggat
    38281 ttgaataaaa aagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
    38341 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
    38401 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
    38461 caggcaattt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtgtgaa
    38521 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacgatg atcatcggtt
    38581 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
    38641 caaagtcaag gatagccaaa acaaattctg catttgtcaa ctcttccata tctttttcgt
    38701 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
    38761 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
    38821 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
    38881 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
    38941 tggattgttg gaaaagatca cttgatccaa ccatttttta tccataaaat cctcttttgc
    39001 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
    39061 tggcttatca ggtgtcaggc cgatcgctat ccaagaaaga agttgcgaaa attcctccaa
    39121 tgtacctaga cctccaggga aaacgaggac agcatctcct ttttcaaata ataatacctt
    39181 tctttcatca attgtctctg taggaataaa tgtagtaaca tctttcccag gcagctcagt
    39241 ttcaaaaagg ccatttggat agatacctgt gacttttccg ttattttcca gtactgtttt
    39301 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
    39361 tgccatatat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat tgccaaaacg
    39421 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
    39481 cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat
    39541 agatcaaaca ccactatata tagtatttgc aaaaaaataa tttttcagct attttacagt
    39601 aaccaaaaaa ataaataact agtaaatata aaattgaaac gacttactta tagaagaaat
    39661 tgggatttcg tcctgcatgt caataacacg cattatttaa atagtcacac acttgtttct
    39721 cctattaatg aaaaatccat tttattctcg tgttatgttt caccaacgcc tgatgtttct
    39781 ctaaataaaa aagccgacga accaaataat cgaaatttga tcattttggt tcatcagctt
    39841 gttcccaaac ctttgtatta acgataaata gctggattcg taaaactatc acttggatca
    39901 tcactgctgg aagaaaattc ggtgataacc gcgcctgttg cacctgcctg gaaccagtgt
    39961 ttaatatttt gttcgatcgt gtactgatct ccagcgtgaa gtatgatttc tttttttgcc
    40021 gtatagtaac gttcttgccc ctttggaata atacaggaaa gtttttttgt ttcttttcct
    40081 tcgacataaa gatatactaa gccttttcga caacgaaagg tttccttttt tccttcattc
    40141 tcatctcgag gtgggtgccg atgctctgga caagtttgat aaggcaataa taccagttct
    40201 ttcccacagt atcgctcatt attttcataa acaaataaat tcaatccttc agtctcgatt
    40261 gctcctaatc caaaatctgt atattcgatt ctttctaatt ctgtttcttt caatactact
    40321 cctgaatcta ggaacatttg ttgtactctt ttcttgtaat ccattctgtt cctcctaact
    40381 gattgtcaag tcatcccaag atatctcttc ttcctctttt tcttcttgga tgacttctgc
    40441 atttgggtca actggttttt tcaataataa aatagttatt cccgtagcaa aagaaccagc
    40501 taacaatgca ccaatgaatg cccatggtct ggtcatcgta gcgactgcaa acattcccga
    40561 tgccggaata cggctatctg cgccccaaaa catgctcaat ccaccgccga ctgccccacc
    40621 gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat acgataggga aacatccctc
    40681 tgtgatctga cagatcccca taggaaaagc ggtcttcaat gtatcgattt cttgacgatt
    40741 gaatatattt ttacgaatga ttttccctaa gaaataagcg atcgtcaagc cgaatggggt
    40801 taccatcgaa gctaaaatca atacagtgat tggttcatag attccttctg aaaatcgtgc
    40861 cacaccccct tatccagaat aaatggtaat ccagaaacag ccgggtatct gcggtaataa
    40921 gcctaaccaa acgaaaaata tgagaagcta atatgagaag ctatttttgt ttggctgttc
    40981 ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc
    41041 aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc
    41101 aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat
    41161 ttcttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt
    41221 atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa
    41281 ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata
    41341 atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt
    41401 ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc
    41461 caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac
    41521 cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc
    41581 ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggtaa
    41641 catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc
    41701 caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga
    41761 atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca
    41821 ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca
    41881 aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag
    41941 ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc catacatccc
    42001 caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata
    42061 tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga
    42121 tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat
    42181 tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca
    42241 agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca
    42301 gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct
    42361 tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt
    42421 ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa
    42481 aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg
    42541 ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg
    42601 ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt
    42661 tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag
    42721 gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat
    42781 actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat
    42841 tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca
    42901 tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta
    42961 ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat
    43021 cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc aattcctcga
    43081 tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata
    43141 atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagccc ttttccgtct
    43201 cgatcataag ccccttctaa ttgattggca gcagttgctc caccccataa aaatttttct
    43261 gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa catcttcgtt
    43321 tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag tattcgtaat
    43381 gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt caaaacgaat
    43441 caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa agccattccc
    43501 atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt cagtcgttgt
    43561 gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg caggtgcaac
    43621 tagttctcct acttctggat caatagcgat accttttccc atcacgccag aagaaaaaac
    43681 ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt ttaaggtatg
    43741 ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg ttttctaatt gattgtcttg
    43801 tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa aagcaattcc
    43861 tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat taatgaaagt
    43921 cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg aaaatccgat
    43981 aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct tcaaaggtaa
    44041 tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg tagaagaaat
    44101 ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag cagccccacc
    44161 ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat attgttcgat
    44221 attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga atacttgcca
    44281 taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg agttgaagag
    44341 accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa atgttaatgg
    44401 aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt gcggaatcac
    44461 tttttttacg aatggctcta atttactttg aacccatacg gctagaatga ttggtatgac
    44521 agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac tgatagcttc
    44581 tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa ttgctactgc
    44641 taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga caggtaagaa
    44701 ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat cagctgtcag
    44761 ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg ctgccatcgc
    44821 ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca aaatattttt
    44881 ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga tcgattgata
    44941 aacatctgct acattactgc caatcactac ttgatactga ccgccacttt gtacgacttg
    45001 gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat cttttaatct
    45061 gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc ctcctaccgc
    45121 ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct ttctttttta
    45181 tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga acctattttt
    45241 tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc tttttttctc
    45301 acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc ccatttcctg
    45361 tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg cttttggata
    45421 ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt cccctttgag
    45481 ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca aagattcttc
    45541 atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta ccattgtggt
    45601 agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa aatgaaaggc
    45661 aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca cgaacaaaag
    45721 agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa ataataatgg
    45781 attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat gatcggtcaa
    45841 tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca gttcgactac
    45901 ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct ggaattggtt
    45961 tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg aaatctcgtc
    46021 cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt ccccgaatcc
    46081 atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt tgcttcgccc
    46141 tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga attctggttt
    46201 tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat cgaattttta
    46261 tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct atctagaaaa
    46321 caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat tggtttttcc
    46381 ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc cagtatccta
    46441 aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat cttttatcga
    46501 tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca tgtcgccaca
    46561 agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg tatctgtagg
    46621 agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat ctgtcaaata
    46681 gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag ggttcgttgc
    46741 acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag ataatgttcc
    46801 tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat tgattgaaac
    46861 tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt gcgacctcat
    46921 gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat ctttatcgtt
    46981 attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc ctgtctctct
    47041 gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta ataacaagag
    47101 gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca ttttctctcc
    47161 ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg acacatttct
    47221 acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat ttcaaattat
    47281 ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg tctgccatca
    47341 tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat tgtgtttctt
    47401 ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct tccaaagaaa
    47461 tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca tctctaaact
    47521 ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc ttacttgatt
    47581 aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact cctgttttct
    47641 tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat gtgtatgcag
    47701 tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt cccaaacaga
    47761 caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt actctacttt
    47821 aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta actctcttaa
    47881 ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc acgcctaaaa
    47941 taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat tttgacgatc
    48001 gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt tcgtactaat
    48061 actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa acagaagggt
    48121 gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc tctatctccg
    48181 tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt tgctctttct
    48241 agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag
    48301 ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt
    48361 ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc
    48421 cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg
    48481 actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat
    48541 aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg
    48601 gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga
    48661 tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc
    48721 taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga
    48781 aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt
    48841 tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa
    48901 cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag
    48961 ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa
    49021 taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa
    49081 tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc
    49141 aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag
    49201 aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag
    49261 gtttttcgta gtaaaataaa aaagaaacat caataataag ccaattaggt aaggaaagct
    49321 cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt
    49381 ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa
    49441 ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg
    49501 tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac
    49561 cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg
    49621 cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac
    49681 gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa
    49741 caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt
    49801 gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat
    49861 ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta
    49921 aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa
    49981 attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca
    50041 gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct
    50101 tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct
    50161 gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt
    50221 gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga
    50281 gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga
    50341 agtcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga
    50401 atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt
    50461 gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg
    50521 ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag
    50581 ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt
    50641 ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt
    50701 ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta
    50761 cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat
    50821 atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa
    50881 tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat
    50941 catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg
    51001 tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg
    51061 tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt
    51121 tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac
    51181 aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc
    51241 attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt
    51301 ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc
    51361 taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat
    51421 tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa
    51481 catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg
    51541 atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat
    51601 caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac
    51661 gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc
    51721 tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc
    51781 cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga
    51841 ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac
    51901 tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg
    51961 ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa
    52021 atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag
    52081 gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat
    52141 caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat gttatctctg
    52201 ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt
    52261 tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt
    52321 ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct
    52381 gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg
    52441 gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa
    52501 atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa
    52561 gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat
    52621 gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct
    52681 tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata
    52741 catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa
    52801 tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac
    52861 ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt
    52921 caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac
    52981 ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat
    53041 tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg
    53101 ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg
    53161 tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg
    53221 gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg
    53281 ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt cgattataat
    53341 ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata
    53401 cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg
    53461 ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat
    53521 gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg
    53581 tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat
    53641 tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta
    53701 aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg
    53761 ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg
    53821 aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat
    53881 tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat
    53941 tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct
    54001 gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta
    54061 caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt
    54121 gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag
    54181 ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc
    54241 tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa
    54301 attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct
    54361 ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta
    54421 agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac
    54481 cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat
    54541 agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc actggttgga
    54601 catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc
    54661 ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat
    54721 gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat
    54781 ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact
    54841 gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat
    54901 caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc
    54961 ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt
    55021 cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt
    55081 gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag
    55141 cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt
    55201 caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg
    55261 atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt
    55321 gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta
    55381 tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg
    55441 tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta
    55501 tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa
    55561 acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca
    55621 tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta
    55681 aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta
    55741 agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact
    55801 tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag
    55861 gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt
    55921 gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc
    55981 ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac gacattcata
    56041 aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga
    56101 ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta
    56161 cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct
    56221 tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct
    56281 tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca
    56341 aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct
    56401 gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt
    56461 tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga
    56521 aacattcatt tattttttga caagataaaa catccatact atgaaactga ggaaaattaa
    56581 aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat
    56641 cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt
    56701 tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg
    56761 atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag
    56821 tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt
    56881 acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa
    56941 taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt
    57001 tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa
    57061 atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta
    57121 ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac
    57181 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
    57241 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
    57301 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
    57361 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
    57421 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
    57481 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
    57541 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
    57601 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
    57661 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
    57721 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
    57781 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
    57841 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
    57901 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
    57961 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
    58021 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
    58081 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
    58141 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
    58201 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
    58261 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
    58321 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
    58381 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
    58441 attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt
    58501 taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata cttaatacca
    58561 accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg cacttgcata
    58621 ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt tcgcttttgc
    58681 accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc tcgtacaagt
    58741 tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc tagaaatttc
    58801 ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat gaatctctcc
    58861 tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca cttcagatga
    58921 catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg cttttttcat
    58981 aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc ctattttggt
    59041 cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt gttgcgataa
    59101 gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt catcggctaa
    59161 aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc
    59221 agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc
    59281 atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa
    59341 attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagatatggt
    59401 atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt gatcgaacaa
    59461 atagaattgt cctgaactga actgatcatt caatcctaat atattcaata acgtactttt
    59521 acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag tgaaacgatc
    59581 caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg
    59641 ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat
    59701 aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt
    59761 aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt
    59821 catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc
    59881 gattttccga caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa
    59941 agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt
    60001 gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg
    60061 actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa
    60121 cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt ttttcaatgt
    60181 cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa atgtataaat
    60241 gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt cttgataata
    60301 ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt cagggatcaa
    60361 tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga catcaatcaa
    60421 aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact gttcattttc
    60481 ttcattcaag taatctgata cattgactaa tatacttccg tttttattca aagtttcata
    60541 taattcctga tctgctgctg atgcaaaacg attggaataa ataaaatcca cgtggttttt
    60601 cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt ttggtggttc
    60661 atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga cattggcaac
    60721 ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga tatagttgaa
    60781 cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag agataccata
    60841 tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct ccttataata
    60901 cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga ttttcttttt
    60961 ccccaataaa caccacaata cttgatgtaa tgaaaagtca ttattcaaca ataaactgat
    61021 ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa tcaatccaat
    61081 agttaaatac actcgataat tatatacgtc tgtatttgaa ataaccgaat acgaatagct
    61141 attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt gattgaatcg
    61201 atcagtgaat agtgaaatga cccgttcata ggcttctttt tctctcgtca tcaagaaaaa
    61261 ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc gaaaggaggt
    61321 attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat acaatgtcat
    61381 ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg attcatttac
    61441 tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata ccatgattag
    61501 ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag tataagtatc
    61561 tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttatac taaccaaggc
    61621 aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact tttttttgat
    61681 tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt tttcatcaaa
    61741 aaaacgtatc tatcaagtgt ataggtcatg tagaataaat ctatatactg tagaatcgaa
    61801 aggagaagaa gatgaacacc ggagaaaaaa tacgaactta ccgcagaaat tgtaagctta
    61861 cgcaaaaaga attaggcgaa aaaattggtg tttccgataa aacgatttct agttgggaaa
    61921 attctcgtac aatgccagac tttgaaatgc tgtcactttt acatcatact ttaggtctgc
    61981 cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca ttgccagatg
    62041 aacctcttgt taacaagaaa tcttattcat caaaacaacc cttctcactc aaaaagacac
    62101 tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat tatcaataca
    62161 aaaatgaaac gatcgatcga ttcaatcctt ttttgaaacc acaaatcggc tataccacac
    62221 tccctgtagg atacgtggac aattccatcg aacgttgggt atgtaatgat atctttggcc
    62281 atggtacatg gctgtctttt cacgctggag aggtagaaaa agagaaaaac catgctgtaa
    62341 tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa gccattccct
    62401 cagattacca agaaatcctc agccaagact atgacgacgc cttaaaaaga agccctcagg
    62461 agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa tgcacatgat
    62521 cttatccaaa aaacaggggt acaaattaat tgaaaaagcc ttgagatgaa agttcctaaa
    62581 taaaaaagtt cagaatgata ggtatttctg gcttatagta taatatttac gccacagcct
    62641 cccaatgatt ctttttggaa tcataatcaa ggatggacat tcacaaaaat attttgctca
    62701 ttcgatccga atagattaac gaccaatcta gtaagtaata tagattggta cgtagtcaag
    62761 ctgcacataa tttagatcaa ttttagttct tttttatatt tctatgatct gctcatttac
    62821 ttttatatga tggcttacag taacataaat aaatatgata atcgctggta gtttatccaa
    62881 gagtactcaa taaaatacaa aatggattgt tcaagcgcct atcataatga taaaatgaaa
    62941 caaataaacg atacaaggag gaagagactt tgagtaatgg cagaacaatt ttttcattgt
    63001 aaaaattgtt cagttgttaa tctatgggat ttcggtagca tgggcatttt tttatagagg
    63061 aatcacacca acctggccgt tttatctctt gttagtggga atattagtag gaaattttgc
    63121 ccctcttcac tatcgtacga acttttttcc aactgattct ggcctttttc ttaaaacaaa
    63181 gacgcgaaaa ttagaaaaca ttattgaaat gacaattacc tgtataatca ttttcttagt
    63241 tatattccta aacgtcttct aataagaatc tcttaaaatt agcttgttac agttagttac
    63301 ctagtttata taacacagtt atcttatctt ccttatcaac atcttttgcc aactctttgt
    63361 cttaaattcg aaacatataa gcagagctac gaacaccaag actgtttcgg ctctgttttt
    63421 tgcatcaaat tcactcttat atagctgata atctcggctc tttaaaagaa attattttcg
    63481 cttcatttta ttataaaaag gctaggaacc ttgat
//
LOCUS       DACDEA010000007        57442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDEA010000007 DACDEA010000000
VERSION     DACDEA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.007"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            118..1995
                     /gene="tdc"
                     /locus_tag="IV136_000468"
     CDS             118..1995
                     /gene="tdc"
                     /locus_tag="IV136_000468"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8464484.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2238..3659
                     /locus_tag="IV136_000469"
     CDS             2238..3659
                     /locus_tag="IV136_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464485.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3768..5135
                     /gene="nhaC"
                     /locus_tag="IV136_000470"
     CDS             3768..5135
                     /gene="nhaC"
                     /locus_tag="IV136_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8464486.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5416..6435)
                     /locus_tag="IV136_000471"
     CDS             complement(5416..6435)
                     /locus_tag="IV136_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8464487.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6457..7464)
                     /locus_tag="IV136_000472"
     CDS             complement(6457..7464)
                     /locus_tag="IV136_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8464488.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7457..8122)
                     /gene="pgmB"
                     /locus_tag="IV136_000473"
     CDS             complement(7457..8122)
                     /gene="pgmB"
                     /locus_tag="IV136_000473"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8464489.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(8115..10409)
                     /locus_tag="IV136_000474"
     CDS             complement(8115..10409)
                     /locus_tag="IV136_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8464490.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10669..12834
                     /locus_tag="IV136_000475"
     CDS             10669..12834
                     /locus_tag="IV136_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464491.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13138..13971
                     /locus_tag="IV136_000476"
     CDS             13138..13971
                     /locus_tag="IV136_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8464492.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14073..15773)
                     /locus_tag="IV136_000477"
     CDS             complement(14073..15773)
                     /locus_tag="IV136_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464493.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            16280..17440
                     /locus_tag="IV136_000478"
     CDS             16280..17440
                     /locus_tag="IV136_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8464494.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17456..17899
                     /locus_tag="IV136_000479"
     CDS             17456..17899
                     /locus_tag="IV136_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464495.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18382..18912)
                     /locus_tag="IV136_000480"
     CDS             complement(18382..18912)
                     /locus_tag="IV136_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8464496.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            19017..19568
                     /locus_tag="IV136_000481"
     CDS             19017..19568
                     /locus_tag="IV136_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8464497.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19721..20194)
                     /locus_tag="IV136_000482"
     CDS             complement(19721..20194)
                     /locus_tag="IV136_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8464498.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20332..21804
                     /locus_tag="IV136_000483"
     CDS             20332..21804
                     /locus_tag="IV136_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8464499.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21801..22511
                     /locus_tag="IV136_000484"
     CDS             21801..22511
                     /locus_tag="IV136_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8464500.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22863..23147
                     /locus_tag="IV136_000485"
     CDS             22863..23147
                     /locus_tag="IV136_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8464501.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23428..23847)
                     /locus_tag="IV136_000486"
     CDS             complement(23428..23847)
                     /locus_tag="IV136_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8464502.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(23849..24433)
                     /locus_tag="IV136_000487"
     CDS             complement(23849..24433)
                     /locus_tag="IV136_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8464503.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(24480..25955)
                     /locus_tag="IV136_000488"
     CDS             complement(24480..25955)
                     /locus_tag="IV136_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8464504.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26136..26447
                     /locus_tag="IV136_000489"
     CDS             26136..26447
                     /locus_tag="IV136_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464505.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26562..28898)
                     /locus_tag="IV136_000490"
     CDS             complement(26562..28898)
                     /locus_tag="IV136_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464506.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            29040..29708
                     /locus_tag="IV136_000491"
     CDS             29040..29708
                     /locus_tag="IV136_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464507.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29711..30754
                     /locus_tag="IV136_000492"
     CDS             29711..30754
                     /locus_tag="IV136_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8464508.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(30991..31872)
                     /locus_tag="IV136_000493"
     CDS             complement(30991..31872)
                     /locus_tag="IV136_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8464509.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            32031..32759
                     /locus_tag="IV136_000494"
     CDS             32031..32759
                     /locus_tag="IV136_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8464510.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32915..33232
                     /locus_tag="IV136_000495"
     CDS             32915..33232
                     /locus_tag="IV136_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8464511.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33225..33833
                     /locus_tag="IV136_000496"
     CDS             33225..33833
                     /locus_tag="IV136_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8464512.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33849..34679
                     /locus_tag="IV136_000497"
     CDS             33849..34679
                     /locus_tag="IV136_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8464513.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            34817..35305
                     /locus_tag="IV136_000498"
     CDS             34817..35305
                     /locus_tag="IV136_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8464514.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            35356..36228
                     /locus_tag="IV136_000499"
     CDS             35356..36228
                     /locus_tag="IV136_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8464515.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36404..36766
                     /locus_tag="IV136_000500"
     CDS             36404..36766
                     /locus_tag="IV136_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8464516.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36775..37527
                     /locus_tag="IV136_000501"
     CDS             36775..37527
                     /locus_tag="IV136_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464517.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37541..39343
                     /locus_tag="IV136_000502"
     CDS             37541..39343
                     /locus_tag="IV136_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464518.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39620..40660
                     /locus_tag="IV136_000503"
     CDS             39620..40660
                     /locus_tag="IV136_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8464519.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(40712..42856)
                     /locus_tag="IV136_000504"
     CDS             complement(40712..42856)
                     /locus_tag="IV136_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8464520.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(43213..44586)
                     /locus_tag="IV136_000505"
     CDS             complement(43213..44586)
                     /locus_tag="IV136_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464521.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(44586..44981)
                     /gene="rbsD"
                     /locus_tag="IV136_000506"
     CDS             complement(44586..44981)
                     /gene="rbsD"
                     /locus_tag="IV136_000506"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8464522.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(45009..45926)
                     /gene="rbsK"
                     /locus_tag="IV136_000507"
     CDS             complement(45009..45926)
                     /gene="rbsK"
                     /locus_tag="IV136_000507"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8464523.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(46034..47047)
                     /locus_tag="IV136_000508"
     CDS             complement(46034..47047)
                     /locus_tag="IV136_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8464524.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(47128..47727)
                     /locus_tag="IV136_000509"
     CDS             complement(47128..47727)
                     /locus_tag="IV136_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8464525.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            47913..48614
                     /locus_tag="IV136_000510"
     CDS             47913..48614
                     /locus_tag="IV136_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464526.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            48624..51335
                     /locus_tag="IV136_000511"
     CDS             48624..51335
                     /locus_tag="IV136_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8464527.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            51786..53414
                     /gene="treC"
                     /locus_tag="IV136_000512"
     CDS             51786..53414
                     /gene="treC"
                     /locus_tag="IV136_000512"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8464528.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(53474..54655)
                     /locus_tag="IV136_000513"
     CDS             complement(53474..54655)
                     /locus_tag="IV136_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8464529.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            54907..56430
                     /locus_tag="IV136_000514"
     CDS             54907..56430
                     /locus_tag="IV136_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8464530.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(56499..57227)
                     /locus_tag="IV136_000515"
     CDS             complement(56499..57227)
                     /locus_tag="IV136_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464531.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(57240..>57442)
                     /locus_tag="IV136_000516"
     CDS             complement(57240..>57442)
                     /locus_tag="IV136_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8464532.1"
                     /translation="RVINTTTNTLPLNQLKQKGFSTKTNPKNEGLGLFILDELTKTNP
                     YLFLETSIENQRFSQTIYIQIK"
BASE COUNT        17974 a        10301 c        11757 g        17410 t
ORIGIN      
        1 attgatacat attaaaatat gattaaaaat caggccaaaa cttagtttac agaatagaag
       61 aacaggtgta atgtagggct cgacaaaatt taaatatttt tttaggaggg tttcactatg
      121 agtgaatcat tgtcgaaaga tctaaaccta aatgccctat tcattgggga caaagctgag
      181 aacggccaaa tctataaagc tttgttgaat gaattggtgg atgaacattt aggctggcgt
      241 caaaactaca tgcctcaaga catgcctatt attacgccag aagaaaaaag cagtgctagt
      301 tttgaacaca ctgtgaataa aacaaaggat gttctatcag aaatttcagc gcggatgcgt
      361 acccattccg ttccttggca taatgccgga cgttactggg gtcatatgaa ctctgaaaca
      421 ttgatgccat ctttactagc gtacaatttt gctatgctat ggaatggtaa taacgttgcc
      481 tatgaatctt ctcctgcaac aagccaaatg gaagaagaag taggaatgga atttgcaaaa
      541 ttaatgagct ataaagatgg ctggggccac atcgttgctg atggttcttt agcaaactta
      601 gaaggacttt ggtatgcccg caacatcaaa tcattgccgc ttgcaatgaa agaagtgaca
      661 cctgaactag ttgctggaaa aagcgattgg gaattaatga acttgtcaac agaagaaatc
      721 atgaatctgt tagacagcgt cccagaaaaa atcgacgaaa tcaaagctca ctcagcacgt
      781 agcggaaaac atttagaaaa attaggaaaa tggttggttc cacaaactaa acactattca
      841 tggctaaaag ctgccgacat catcggtatc ggtttagacc aagtgattcc agtgccagtt
      901 gaccacaact atcgcatgga catcaacgaa cttgaaaaaa tcgttcgtgg tctagcagct
      961 gaaaaaacac caatcctagg tgtagtcggt gttgtaggat caacagaaga aggagcaatc
     1021 gacggtatcg ataaaatcgt tgcattgcgt cgcgtattag aaaaagacgg tatttacttc
     1081 tacttacacg tagatgctgc ttatggcgga tacggccgtg cgattttctt agacgaagac
     1141 aacaacttca ttccatttga agatttaaaa gatgttcatt acaaatacaa tgtctttaca
     1201 gaaaacaaag attatatctt agaagaagta cacagtgcat ataaagcaat cgaagaagca
     1261 gaatctgtaa cgattgaccc acataaaatg ggctatgttc catattctgc cggtggtatc
     1321 gtcatcaaag atatccgcat gagagacgtt atctcttact ttgcgacata tgtatttgaa
     1381 aaaggcgcag atatcccagc attacttggg gcttatatct tggaaggttc aaaagcaggg
     1441 gcaacagcag ccagcgtttg ggcagcacac catgtcttac ctttgaatgt cacaggatat
     1501 ggtaaattaa tgggtgcatc aatcgaaggt gcacatcgtt tctacaactt cttgaatgac
     1561 ttgtcattta aagtaggaga caaagaaatc gaagttcatc cattaactta tccagatttc
     1621 aatatggtag actatgtatt caaagaaaaa ggcaacgatg acttagttgc aatgaataaa
     1681 ttgaaccatg acgtatatga ttattcttca tacgtgaaag gaagcattta cggtaatgag
     1741 ttcttaactt cacatactga ctttgctatc ccagactatg gcaacagccc attacaattt
     1801 gtgaaccaac taggattctc agatgaagaa tggaaccgtg ccggaaaagt aactgtattg
     1861 cgtgccagcg taatgacacc atacatgaac aaagaagaac actttgaaga atatgctgaa
     1921 aaaatcaaag cagctcttca agaaaaattg gaaaaaatct acgcagacca attattggca
     1981 agcgaagcaa aataagcaag gtaatcatgt tagaaaacag cttgaatact aactgtttct
     2041 gacgcatgtt tggatctgaa gagcaagggg agacctaagc tcttcgatcc ctttgttttg
     2101 aggttgtctt tgaagaaacc cttcaagcaa taaactcaaa gaatcccctc aaatattcgc
     2161 tgaattgttt cgtttattgc tgaaactact tcaaagacac cgttatttta tccaaaaaat
     2221 caaggagagt gtcaacggtg agtaaatctg aagtaagtaa aatgggtctt gggaccttta
     2281 tcggtctgac aatggcactg tgtgcaacgg ttcggagtat tcctacgtta gccgcagtcg
     2341 gttggacatt gattttttat tcaatttttg cagttttgtt ttttgccggt ccgatatcaa
     2401 tggtttccgg tgagctttcc acgatgcttc ctcaagaagg cggtcctcag ttatgggtaa
     2461 aaacagcact gggcagcaaa tggggatttg tcgttgcatg gctattatgg gtgcaaatgt
     2521 tcccaggtat ggtaatggtc gcttctactt taggaccatt gttaggaaac acatttggta
     2581 atgtagaact tggcaataat cactggttcg ttttaggttg tattttagtt atctactgga
     2641 tcatcacgat cttgaacttg aaattcgata tggcaaaagt cggcgggaac atcggtgtat
     2701 ggctaggggt atatatccca gtagtcatca tgttcgtttt aggtgttttg gcagccttca
     2761 aagtcggact agtctcaaac ggctatctag gtgacttttc atggtcaaaa gctttccctg
     2821 acttggaaca tattgattca ttgaaatact tagctggtat cacatttatt tttgttggga
     2881 tcgaaatgag ttctgtttac atgcctcgtt tgaaagacgc aacaaaaaat tatacaaaag
     2941 gcgtatttat cgctttgatc ggattagttt tattgaatgt catcaatgcg atgcttgtag
     3001 caaatgtcgt accagatgga aaaatggaat tagcaaatat cacgcagcct atcttgattg
     3061 actgtcaaat cctaggctta ccagaagtga tcggaaatat cttcagcttc atggtattta
     3121 ttggtgtgct tttacaatta tcagcttggg ttactggacc atcaaaaaca atcatccaag
     3181 tagctcgtga aggattcctt ccaccaaaat tcggattcca taaagaaaac aaatacggcg
     3241 tatctagaaa tgtcgttcta acacaatcaa tcgttatttc attatttgct ttgctttatg
     3301 gtgtaatgga tgacgtaagt gctgtattct tgacacttac aaatgctaca actgttattt
     3361 attgtatcgt gtacattttg attgctgttt cattgcttaa aatgcgtaag aaacatccag
     3421 aattcgaacg tccttaccga attggtaaaa acggaaacgg attagcttgg gtagtcagct
     3481 gcatgctgat cttctctatc atcgttgtcg tatttgctac attaggtaca gcgacattga
     3541 gtgatgcatt attagtagca gctatcacag tggtcatgtt cgttattcca ttgatcatca
     3601 atcacttcaa gaaagattct tgggggattg aggtagaaaa aagtttagaa gaaaaataaa
     3661 aaaataagtc tttcagccga cgttttgtcg ctgatttagt aggaggtcct gccaagtgaa
     3721 tagttttttc atggcagggc cttttcattt gaaataggag atcgacgatg aataaaaata
     3781 tttctttaaa agaaagtatc gctgtattga tcgtgttgtt ggcactccta ggagtactga
     3841 tcatcggatt ccagctctcg ccacatatcc cgatccttgc agctttcatg tgtttgttgt
     3901 tttatggcag atttaaaaag ttcacatggg atgagatcca tgacgggatc atcaaaggaa
     3961 tcactccagg gatcattcca atcctgattt ttcttttgat tggtgtctta gttgcttcat
     4021 ggattttatc tggaaccatc cctacgatta tggtttatgg atttaaactt gtttctgtgc
     4081 gtttcttttt accgacagca tttcttgttt gtgcaattgt cggagtgact gtcggcagtt
     4141 catttacaac gatttcgacg attggaattg cttttttggg gatcggtcat ttacttggat
     4201 tcaatgatgc aatgactaca ggcgcagtcg tttccggtgc ctttttaggg aataactgct
     4261 cgcctttatc agatacgacc aacttggcag cgggtattgg aggagttaat ctattcgaac
     4321 atatcgctgg tatgcgttat acggatttcc cagcattcgt cctttcgttg attttttata
     4381 cgatattagg acataacagt cattcagccg atctagctag tatcaatgaa atgatccaaa
     4441 acatgaaagc gagcttttgg atctctcctt ggacattgat tccactagtt attttatttg
     4501 ggggggcaat caaaaaaatt ccggctatcc ctacattact agcgggatcg acagctgctc
     4561 tcgttctttc ttttatccat gagaaaagtt tgacggtcgg aaaagcagca gacatcttga
     4621 tgaatggtta tgtcgcaaat acacctgatc caaagctaga tgaactgctt tctcgaggcg
     4681 ggatcatgag tatgttaggt tctgctagtt tgattttact tgcattggca ttaggtgggt
     4741 tactgatcaa atatctgatc gttgatacca tcgttcaaga attaaaagaa aagatggacc
     4801 gaccttctcg tttagtcggg tttactgcct tgagctgtat cggaatcaat ctgatcgttg
     4861 gagaacagta tctgtccatc attcttccag gagaaacatt caagcgttct tttgatgacg
     4921 ttggcttatc aaaaacctat ttgacacgaa cactagcgga ttcaggtgcg accgtcaatt
     4981 ctctcgtacc gtggggagtc agcggcacat tcattatggg aacgatgaaa gttagcgcgt
     5041 tgcattactt gccattcgcc tttttctcga ttcttgcacc tgtctttaca atattaggcg
     5101 gttttttatt gaatcgtcga cgtgtaaaaa attgaaggaa ggaaaaaaga ggctgggaca
     5161 gaagcgttta actccaagaa ataagaagaa actcaagaaa attgcttttt aaatttttgt
     5221 gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac gtgtttagag
     5281 cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttacagat aaacaatgtg
     5341 ttagaagtag tatcttcttt tacataaaaa gctgaccata ggataaatgg tcagcgtatt
     5401 tagtgagtca gctatttagt atgggttttc taaaggaacg actgtctctc ttttgatcaa
     5461 gcgatgaggg atgaccaaac gtacgccact gctcaattct tcatggatca attcgcgcag
     5521 cttttgcata gcgacacgtc ctaattcgga gatatcgata tcgatacttg taaggtaagg
     5581 gtgtgttaat gttgagaaga tcgagttgtt gaagctaatg acggagagtg tctctggaat
     5641 gacatagcca tacatttgcg ccaactgcat cgtgcgaagg gcgaagatat cgtcaatgac
     5701 caccaatgct gtggcttcgt tttctttcaa cgttttctca aaggacagat aatcctccgg
     5761 atgctctagt actacactgg ataacgcagg aagaccagcc atcatcattg ctttttgata
     5821 accgaaatat cgttcgaaat acaaactttc atgagtcgtg tttgtcacaa acaggatatt
     5881 cttatgtcca ttctggatca aaaattctgt tgcttgttta cctagaagct ggttatcatt
     5941 atctacataa acgatttcgt tctcatgatg ataaggctgt ccgatcaaag tgaaagggat
     6001 gtggttttgg tataaataat caatgacagt atcttgacta tctgaataca ctagaataaa
     6061 accatctact tgtttttgta aatgcatacg ctggacgttc tctaacagca attcaaaatt
     6121 tttagcagta gcgattgctg tagtcatatc ataattccgc gcttcttcgt tgattgcttc
     6181 catgatctca agataaaatg ggttaccgat ccgctctttt gaatcaagag ggggtaagat
     6241 tacgccgatg gcactggaaa tgcgttttcc tagattgcgg gcagtcatgt taggaacata
     6301 acctagttca tccataattt ttcggacttt cttctttgtt gtctcggaaa tactaggatg
     6361 atcgttgatg acacgagaga cggtcgaggt ggcaacaccg gcttttttcg ccacatcttt
     6421 tacggtaata gccatgataa aaacaccttt ctcttgttat tgtgcagaga accgataaat
     6481 cgtctgactg atccgttctt gaccaggatg gaggttgatc gatccaaatt tggggaaatg
     6541 aacgatatca gggatttctt gcggttcgat tgctaatccg tagtttgaat gcatctgttt
     6601 tccattgaca gagaaatcag cttcgaatcc agtcgtagaa aataatacca tacttttatt
     6661 agaagttgct atagtcattt ggcgaccaga atgcttctcg aataattgta aggaagtttt
     6721 ggaaagccta ggatgatgca aaagaaatac atcatctagt ccatctggat aacatgcaag
     6781 gatctcttgc agtgttgggg attttctaaa gtcgattggc agtttttctt tttttgtcca
     6841 atttccagta ggcagttttt cttgatctaa ttcaagcaca ccatctaaaa agaccgttaa
     6901 ctgatgttca tagatgtctc ttgttccatt tcctgaaagg ttgaagtagg catggttggt
     6961 cggattgcag atggtttcaa caaggctact agcagaggtt gtcatctcta attcgttttt
     7021 tgttaaacgg taagtgatcg tagcagtgat tggtcctgga taggtagaaa attcatcttt
     7081 taagtaaaag accacaccta ttgatgatgg agttttgaaa acttctactt cccaaaattg
     7141 gaaagaccag cctttcgtgc caccgtggat atggtgtgat ccgcagtttt tctccaatct
     7201 atgctcaccc caagaagcat cccggatcct tccggctaca ggtccgacag ttgctccaaa
     7261 aaagcttttg tctgttagaa catcttcgaa acgatcgtaa gacaataaaa cattttctga
     7321 ttttccttct ttgtctggtg cgaagatttg gtgcaggcgg gcaccgtaat tgagaagaac
     7381 gacttctaag tagtcgttct tcaatgtgat tttctttatt tcagtctctg gaactggtac
     7441 gacggagatc tctggattat ccatggtcat gccacttttc tttcaagtaa tcgatcgtaa
     7501 gaaaagtcgt atcagggaca agcgggatct cctctccaag gtcttcagca cgtcccacgc
     7561 caattggttg agcgccactt gagaggatgg cttggatacc ggcttgcgcg tcttcgatcc
     7621 cgatacattc ttctgctttt agtccgacag cttttgctgc aaggaggaag atatctggcg
     7681 caggttttcc gtttgttgct tctgccgggt cggcaatagc atcaaagtaa ggtgtaagac
     7741 ccattttttc aagtaggaat ggcccgtttt tacttgcaga agcaagagca atcttgattt
     7801 tttcttcttt taaagaatcc agtaaaggca cgaccccggg gaagacatct tttggttcga
     7861 tcgtctggat catctctaaa taataatcgt tttttctttt cgctaattct gcaaattctt
     7921 cctttgaaaa agagtgttct tttccgccat aagctaaaag caaagagagt gagtcttctc
     7981 ggctgacgcc ttttagctgt tcgttgaatg tgtggtcgat cgtaatgccg atttcctgac
     8041 caagctttcg ccaagcacga taatggaatt cagcagtatc ggtaatgaca ccgtctaaat
     8101 caaacaatac ccctttaaac atttgcttcc tcctttctta gcgttatttc tagacgatcc
     8161 gctaaagaat aagactgttc atagacgtaa atttctagag gagaaccttc taataactcg
     8221 aaagtaactt gttcatctgt gactgagatg aacaataaac gtccgcgata attgatatgg
     8281 aaagaataag ctttccaagt ttttggcaga aatggtgcga atctcaactg atcatgggca
     8341 gttttcattt gagcaaatcc ttggacaatc gacaaccagc taccagtcat ggaagtgatg
     8401 tgcaagccgt cttctgtgtc gttattgtaa ttatccaaat ccaagcgagc tgttcgctgg
     8461 tacatttcta cagctttgct ttccattcct aattctgctg ctaaaatcga atggatactt
     8521 ggcgaaaggg aagactcatg gacagtcatt ggttcataga aatcaaagtt tttttgcttt
     8581 tcttcttttg taaaagtatc attgaagaaa taaatcccct gtagtacatc tgcctgcttg
     8641 atgaagcaag atctaaggat tttgtcccaa gaccaatgct gattcagagg aagatcgctt
     8701 gtgggaatat cggatacagg catgagatct ttatctaaga atgtgtcatg ctggacaaag
     8761 attcctaatt cttcgtcaac tgggaagtac atgttctctg cgatatcttt ccatttggct
     8821 agttcttctt cgctgatagt gatagcagct tctttttgat aagttaaata attttctcgc
     8881 gtgtaacgca agacccaagc tgcgatcgta tttgtatacc aattgttatt gatattgttc
     8941 tcatattcgt ttgggccggt cacaccgtgg atcatgtatt tatttgctcg ttttgaaaaa
     9001 tggacacggt ctgcccagaa gcgtgcgatt tctgctaaaa cttccagtcc ttcatttttc
     9061 aaatattccg tatcgccggt gtaattcgta taattgtaga ttgcataggc aatcgcgcca
     9121 tttcggtgaa tctcctcgaa tgtgatttcc cactcgttat ggcattcgac accagtgaag
     9181 gtgaccatag gataaagcgc gccttttaat ccttgttgct gagcgttgtg gattgcttgc
     9241 ggcagttgat tatgccgata tttcagcaga ttcttagtta cttcaggttt ggctaaagca
     9301 agataaagag ggacagcata cgcttctgta tcccagtaag tggctccgcc gtatttttct
     9361 cctgtgaatc ctttggggcc gatattcaat cgttcatctt cgccgtagta agtagagaat
     9421 agttggaaca aattgaatcg aataccttgt tgtgcttctt cgtctccttc gatgaccaca
     9481 tcggcaagct tccatcgttc tgcccaagcc gctgtttgct cttcttttgc ttggatataa
     9541 ttgtttgtct ggttcgttaa ttctgagaca gctttttctg cttgctgcgc ttctggaata
     9601 tctctactag taagaacgat aatttctttt tccactgacc atgtctcacc ttgagataat
     9661 tctgcagaca agcaaccatg taatgctaaa gtatcttcgt gatagtcagg gatgactgat
     9721 tttccattaa cgaaatggcg cattgctgca gtaacagtaa attgttcaat cccaaagttg
     9781 tttggaatag ttttagtcgt caaatagccg atttcttcta tagttccacg atcaatctca
     9841 ttccaaaaat gttcatcata gttgctgtct tcgttttgaa catttccatc caattttgat
     9901 acgagtttga ctgttccgct accttcaagc atttctatag ttaaatagat ataagctgat
     9961 tcttttttga caatgctcag aaaacgttcg aaggaaatgc gtactttatt agtagaagta
    10021 gtgattgtga atgtacgaga taatacacca tttttcatat ctaattccca gtaaaagtct
    10081 tctggttcca aagaagccaa gtcgatcagc tgatcgttga tcgaaatatc tatcgcgata
    10141 aagttcaagg cgttgatcac ttttccaaag tattctggat aaccattttt ccaccagccg
    10201 acacgtgtct tatcgggata ccagacaccc gccaaataag taccttgatg gtggtcccct
    10261 gagtaagttt cttcaaagtt cccgcgcatc cccatataac cgtttccaat acttgttaaa
    10321 gattcttgca gtcgaagatt ttttttatct aattgtgtcg tgctgatttt ccatggatca
    10381 atagcaaata gtcgtttgat ctgtttcatt tttctcctcc taatgagctg ttttatacgt
    10441 tcataataaa cgcaaccgat tgcacaagtc aatagacaaa agaaaatatt tcatagaaaa
    10501 aagcttaaaa aaataaattt ttaggagaaa attatagaag ggaacacacg tattataggt
    10561 tttatagaat ggtatagaca gaaaaataaa aaataaagca tttacaaaaa agaaaacgca
    10621 cgctataata cgtttaacga aaccgattgc gtaaaggagc taaacggaat gaaaaagata
    10681 tttagttttg agttttggca aaaatttgga aaggctttaa tggttgtggt agcagttatg
    10741 ccggcagctg ggttgatgat cagtataggg aaatcaattc ccttgatcaa tcctgattgg
    10801 acgccgcttg tcacaacggg cggagtaatt gaaaatattg gttgggcaat tattggtaat
    10861 cttcatattt tatttgcttt agcaatagga ggaagttggg caaaagaaag agcaggcgga
    10921 gcatttgcag ctgggatctc atttatttta atcaatcgga tcactggggc tatctttggt
    10981 gtcacaagtg acatgcttgc gaatgaagat gcatttaccc acactctgtt cggtacgaag
    11041 atcatggtta aaggattttt tacaagtgta ttggaagcac cagctttgaa tatgggagta
    11101 ttcgtaggga tcatcgctgg atttgtcggg gcaatggctt ataataagta ctataattat
    11161 agaaaattgc cggatgcttt gtcatttttc aatggaaagc gctttgttcc gtttgttgtt
    11221 atcttatggt caacaatcgt ggcattgatt cttgctgtgg tgtggccaaa cgtacaggct
    11281 ggaatcaata attttggttt atggattgca gaatcacaag aaagcgcacc gattctagca
    11341 ccgtttttat ttggaacatt agaacgttta ctattgccat ttggattgca tcatatgctg
    11401 acgattccta tcaactatac acagcttggc ggatcgtatg agattttatc aggcgcacag
    11461 gcggggacac aagtatttgg gcaagatccg ttatggttgg cttgggccac tgatttagtg
    11521 aatcttaaag gtgcagggga tatgtcgcaa tatgagtttg tgttgcataa ctggacgccg
    11581 gctcgtttca aagtaggaca aatgattggt tcttcaggta tcttgatggg aatggctttt
    11641 gcgatgtatc gtaatgtcga tcctgataag aaagcaagat ataaatcaat gtatttttca
    11701 gcagcgttgg ctgtctttct aacaggtgta acagaaccat tggagttcat gtttatgttt
    11761 gctgctgtgc cattatatgt catttattca gtgatccaag gggctgcatt tgcgatggca
    11821 gatatcttgc cattgcgggt ccattcattt gggaatatcg aattgctgac tcggacaccg
    11881 ttagctatca aagcgggtct aggtggagat cttatcaact tcgtgttgtg tgtgatcgct
    11941 tttggtgttg ttacgtattt cttagcaaat ttcttgatca aaaaattcaa ctttgctaca
    12001 ccgggacgta acggaaatta cgacaatgac agtgaagaaa ccgttgtatc taacagcgga
    12061 acaggaacag cagatcagca agtggtccag atcattcatt tgctgggagg aaaagaaaat
    12121 atcaaagatg tcgatgcttg tatgactcgg ttacgcgtaa gtgtaaatga tcgagaaaaa
    12181 gtagggactg aagaagcctg gaaacgagca ggggcgatgg gattgatcgt caaagacaat
    12241 ggagtccaag cagtttatgg accaaaagca gatgtcttga aatcagatat cgaagatctg
    12301 ctgcaatcag gagcagagat tccagagcca gaaatggttt ctacaggaaa tggacaagca
    12361 ggtagtaagc aatacttagg tatagaacaa gaactagttt ccgcagcttc tggagaagtg
    12421 attcctttat ctgaagtaaa agatccggta ttctcacaaa aaatgatggg tgacggcttt
    12481 gctgttattc caacatcaag agaagttgtt gcgccgatcg ctggaaaagt gacaagtatt
    12541 ttccctagca aacatgcaat tgggctggaa acgaaagatg gtatcgaagt tttgatccat
    12601 atgggaatcg atacagtcca aatgaatcag ccagcttttg agattgctgt caaagaaggc
    12661 caagaagtag gaacaggaac gaaattagct gagatggatc ttgatgtgat ccaaaacgaa
    12721 gaaaaagatc caacgatcat gatcgtattt acaaacgata aagtagaaga agtagtgatc
    12781 aagcaattag gtacagtgac agctggtaaa gtgatcggct ccattaaaat gtaaggagag
    12841 gttgtgaaaa agctgtcctg catcaagtca taagaacagc caaacaaaaa tagctcctcc
    12901 tatttttcgt ttggttgggc ttattaccga gaagcagcag tttgaatact gtttagtgga
    12961 gaggtcgtga aaaagctgcc ctgcaccaag tgataaaaac agccaaacaa aaaatagctc
    13021 ctggtccatt gagcttatta ctgaggagtt agcaggagaa cactatttgc tggagaggtt
    13081 gtgacaaaaa ttttgtcaca acccttttgt ttcgaaatga agacaaggag aaaaccaatg
    13141 aaattcatga cactcaacac gcatagctgg cttgagccag aaccagagaa aaaattgcaa
    13201 gaattagcag ataagatcct gttagaagat tatgaagtga ttgctttaca agaaatcaac
    13261 caattactgg aatctgaaga agtagaacct gcgaagctaa tgaagttctg tccggtaaag
    13321 aatcaagtac ctatccgtaa agataatttt gcctaccgtt tagttcagct tttaaaggaa
    13381 cacggaaaag agtatttttg gagttgggaa atgagccaca tcggttatga taaatatgaa
    13441 gaaggaaatg cactgcttac gaaaaaaaca ttggaaagtc aggtccttac cgtctcccaa
    13501 tttcaaaaga aagaagatta tcagacacgc aagatcttga ttgggaaaac aaagatagat
    13561 gatcaggaca tttttgtctc gagctgccac ttttcttggt ggacagataa aaaaagtggc
    13621 ttttattttg agtggaaaaa cttggaaaac tactttttgg aaactcgcgt accacttttt
    13681 ttcctaggtg atttcaataa tccagtagac tctcaaggtt attatactgt aagagaaagt
    13741 tgccttcttc ttcaagattc gtatgttgtc gcgaatgaaa aaggaaaagc agctactgtg
    13801 gaaaagaaaa tagatggttg ggaacaaaat acagaaaaat tgcgtataga ttttattttc
    13861 gtccctgaag gaatgcaggt taaaaaatat caacggattt ttgatgggat cgatagtcca
    13921 atcatcagtg atcactacgg ggtggaaatc gaattagatg tgaacgaata aaaaatataa
    13981 catataacat aaaaaaactg attaaaataa aactctccaa acgattatac cgtttggaga
    14041 gttttttgat cggatgtatt tatccctttt gcctaagcac gtaaaagctt gtatctatga
    14101 taaataaata aacatagcaa ggtaaaccag ccaatcaaag taagagaaag aatgaaatag
    14161 aaatagattg gcgtttgata atgaagcgtg agctcgttga ttcctttttg ctgcttcacg
    14221 attggggtcc cgatatctgt tacttcataa tcagtcaatg tttttccatt ttgctgtagc
    14281 acagtccgat cgtagacgat aacagggata tgaacagttt tgtccccagt gtttttccac
    14341 ttgattgtca aaagaccgcc tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt
    14401 tcttcgttat accggtcgta tttattgtct tttgtctcct tatagattgg cagatagtca
    14461 ggtgtggttt tttggaaaac gaacaagctg gtagacatat ctttgctgaa gaatgttttc
    14521 cttgcttctt ccggcgtatc gaatagataa gtatgtcgac tgacaaatgt ttcattttcc
    14581 cacttttcca gatgctggta taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca
    14641 aatatactta aaagacggaa ccacttttgt tcagaccact ggttcagtga aagggcaagg
    14701 taaagtagaa gcaatagcgt aaatggcacg aaaaatcgaa atggaaactg gatcaaacta
    14761 ataaaaggca catttttttc gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa
    14821 aagaaaacaa gacttgtgta caacacgatt ttttggaata aggtgttctt ttgttgaaag
    14881 aaaataaaaa gcaaaaaacc tgataatatc aatataggaa aggccttagg gtaatggagc
    14941 caataactgc catccagatc tatcgccttt tgactcattt ctctgttaac aaagggagga
    15001 agaatcttat tcccactgta aacaatagct aatcctgccc aaatatttgc tgtcagaaca
    15061 aaataaagca agaccgactt aatcagttga agcagtgctt ttcctttttc agtctggtta
    15121 aaaaagtaat aaagataaaa agggatatac atcaaagcta acatgatcga agacagtaaa
    15181 tggatctgtg tcataagtcc cactgagaca gcgactttga taacagggaa ctttttatga
    15241 aggacgaaat cagcaatagg gatcatacat aaaggataaa aacaagtccc ccagctagtg
    15301 aatccttggt tgatagacca gtattgaata gaatacgtac tcatgaataa aacggctact
    15361 ggtacactta cccaatttcg aacattgaca tatctcagta aaatcagcat agaaaatcct
    15421 gaaataagat aaagaatgaa atttgaaaga atctgataat gaaaccagct acctgcaagc
    15481 aacacgatca atccttgtaa ataagcgaaa aatgggccgt aaacggcatt cacgatcctg
    15541 ccactttgct ggaatccata aagggaagga aaataatgga aatccccata tttaatttgt
    15601 tgcgcggcat catagaaccg attgtaatgg aatggggcat cagcacccaa aattagttta
    15661 tgtgtgaata aatgaggcat gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg
    15721 ccatattttt tcaattgtcg aactcctttg caaactttag ataaattcat catatatcct
    15781 atgcgagagg taggcaagaa attagaaaat tcatgaaaat atttatgaat tttacaagaa
    15841 aaacataagt gaaagttcta aggagagagg atgcgacata agttttgttg catctccttg
    15901 ttttgaaaca gaatttttct taatactcga aacaaagtag ctttttcatg acctctcggt
    15961 aaacggtgtt caatcagctg cttctcggta ataagctcac tagcctagaa aatatgagaa
    16021 gctattttct gactagcgct cttattactc aaagcataac gcagttttca caacctcttc
    16081 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaacccaac aaaaaatatg
    16141 agtagctatt tttttgggct gtacttatta cttgatgcag gacagctttt tcacaacctc
    16201 atcggctttc attttctatt tttttcctga acccatataa taaaagaaag aacaaaaaaa
    16261 cttgcaaggg aggacacatg tgaagcagag taagcaaaaa cagtctcgtc gacttttgtt
    16321 tcgtttgttt ttattaatat gtatcgtcgt agggttactg atcgctttat atccatttta
    16381 tgtggattct ttaaatagtt ttattgatca aaaacgtatc gaacaagtac aaaaacggac
    16441 cgcagctgaa attgaagtgc agcgaaagaa aatggaggag aaaaaccaac gattaactga
    16501 ccaaggattc aatcctggta ctgatccttt caatgaaaag aacagaaatg aaagtacaac
    16561 atccagccaa ttagaggaat ggctgatcgg aagtatcaat atacctaaga tccaattaaa
    16621 tatttcactt tatgatcggc tcaatggtag gatccttgaa aatggagcag gcgtcttgca
    16681 aggaacctct ttcccgcttg gtggaaattc gacgcattca gtgatctctg ctcacagcgg
    16741 tttacctaat cgacggttat ttaccgaatt agatcgttta gaaaacggag acacgtttat
    16801 tttgactgtt ttgggggaga aattggctta tcaagttaaa aatatacaag tggtactgcc
    16861 agatgacacg tctgttctaa cgatttcaga aggagaagac ttagttacct tgcttacatg
    16921 tacgccgtat atgatcaaca cgcatcgttt gctagtgaca ggacatcgga ttccttataa
    16981 cgaatcggta aaaaaagaag aaggaaaagg aaatcaggaa agaaccatgc ggcaatggtt
    17041 tattcttgca ggaacgataa tagctgtagt catactgtta gtgtttatcg gacgactaat
    17101 ctatcagtat agattaagta aaaaagtgct tgatttttca tttattatta gtgattcgtc
    17161 agggaaaccg gtaaatggta gttcattcat tttgaaacat aagaaaaaaa cacttactag
    17221 aaatggtgtg ccgttttcag tacaaagcga ccatcatggg aaagtcaagc tggatcaatt
    17281 gcctggtgga acttatcgaa tcgtttcagc tgatgatcct aaagttgctg tttcttttgg
    17341 tattagaaaa gtaaaacaag agaagatata cttttttgag gggcgaaaac ttgtaaaaga
    17401 gcttcaaaaa aatggttttt attttaaaat caatgaataa gtaaggagag aaaaaatgat
    17461 ccgcacaaca acaaaattgt ctgccaaaga actggaatct gttctatcga tttggctgca
    17521 tgctaacttg gattctcatg aattcattcc atcaacttat tgggagaatc aagtagaatc
    17581 tgttaggaaa gctttaccag atgcagtgat ttacttagca gaaatagatc aagaaattat
    17641 tggttttgct ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa gggaataccg
    17701 gcaacaaaga atcggtacgc aactaatgaa aaaaataaaa gaaaccaata aaacactgac
    17761 tttgacagtc tatcaaaaaa atcaatcagc tattcatttc tatctttcac aaggttttac
    17821 aattcaaaca cagcaaaaag atgaagcagt aaatgaggag gaacttgtga tgaaatggaa
    17881 tcaaaacgct gatttttgat gaacagaata acgaaataat gctcccagcc taattttttg
    17941 ctgggagcat tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac
    18001 aatctttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    18061 atatgaggaa ctatttttcc atttgtttct ctaatactca ggtcaaaacg ctgatttcac
    18121 aacctcttga taaacggtgt tcaaacggct gcttctcggt aataagccca ctagcctaga
    18181 aaatatgaaa agctattttc taactagcgt tcttattact caaagcataa cgctgatttc
    18241 acaacctctt gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
    18301 aaaacatgag gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt
    18361 cacaacctct ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc
    18421 gctacggata tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc
    18481 tatacctgca tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata
    18541 agtttcatat ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac
    18601 agtgatttct ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt
    18661 ttcatttggc ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt
    18721 gaatacgccg tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt
    18781 gaccattccg ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata
    18841 ttcccaacca ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt
    18901 aaattctttc atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt
    18961 tcgattactc atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg
    19021 caagaaaaaa tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag
    19081 gatatgaatt gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata
    19141 gtcagattta tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag
    19201 agatcacagg tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac
    19261 aattggcaga gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg
    19321 ttctgaaatt gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc
    19381 agcagcgtga ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct
    19441 ttcctactga aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga
    19501 tcaatcggga aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa
    19561 aaaaataaaa aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag
    19621 atcaccgttc ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc
    19681 tacttattaa aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact
    19741 gttcgtagtc agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt
    19801 tgtcatgaat gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg
    19861 tatagcgttc gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat
    19921 ggtgcatttt cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt
    19981 catctaaata ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag
    20041 gacaattcgc atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt
    20101 gacgtggaga cacacctacg atatcactta attcactgat gccaacaacc aattgatcat
    20161 tttccaacaa acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc
    20221 ttcccttaat tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta
    20281 ggactaaagt attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac
    20341 aaccaacccg tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta
    20401 ttaattggta cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca
    20461 ttgatgaaag ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg
    20521 ctagtcaacg ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga
    20581 aatttatatc tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca
    20641 cctaatttta gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg
    20701 atgccattat tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca
    20761 atggggatgt cagggatcgt tattggactt gctcctgctt taggccctac attatctggt
    20821 tatatcattg ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt
    20881 cttgtcttgg ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc
    20941 attgatattt tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt
    21001 tctagtgtag gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt
    21061 gtgattttca tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa
    21121 cttcgtgtat tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat
    21181 atggcgatgg taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa
    21241 tcagcattcc attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg
    21301 cctgtgacgg gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg
    21361 ttcattttga cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta
    21421 tatatcgtga tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta
    21481 acgacttctg gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat
    21541 aatacattcc gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg
    21601 aatgtgacaa aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat
    21661 aaagatcagg caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc
    21721 atctttggga ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta
    21781 atgaaagcgg gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg
    21841 ctttaacgaa cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac
    21901 taatcttcgt cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg
    21961 aaaaagtaac ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag
    22021 atatgctttt gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa
    22081 atgaaaaaca agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa
    22141 agacagacgg cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca
    22201 tgtataaatt ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata
    22261 cattcaaaat cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg
    22321 aattagtgaa gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg
    22381 gtatggtaaa agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta
    22441 cgaaagcctt agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa
    22501 aaggcgaata atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatcaag
    22561 cagtaagaac aatcagataa aaatagcact ccatattttt catctgattg ggcttagtgc
    22621 cgtagatgca agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc
    22681 tgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgttgacta ttgtgacttg
    22741 tgaccgagga gttggcagga gaacaccgtt tattcgaaaa aaggaactgc gacaagactt
    22801 ttgtcacagt tccttctttt ttttgctatg ctatactaga tgtaaaaaat aggagaaata
    22861 aaatgaggga aatttattct tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg
    22921 gggcatatat tatttttccg tatgatataa gaaaagaatt cggtgctgga agagttaaag
    22981 tccaagcaac ttttgacaat gtgccatatg aaggcagtat cgtgaatatg ggcgtgaaaa
    23041 atccagatgg ttcagtctgc tacatccttg gactacgaaa agatattcgt aaaaacatcg
    23101 gaaaagaaat cggtgatatt gtagcagtta cagtgaagca aaaataaaag aggttgtgac
    23161 aaaagacgtg tcacaacctc ttgataaacg gtgttcaaaa gccagtttct cggtagtaag
    23221 tcaaatttat gcaaaaatgt ggaaacccat gtttacaaaa tttttcttac tactcgaaac
    23281 gaagcggctt tatcacaacc tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta
    23341 gctcaaaaac ggcaaaatat gagaagctat ttttccattt ttttctctca tactcaggtc
    23401 ataacgctga tttcacaacc tcttctttca taatggtttt tcaccatctt tataataaac
    23461 tggttctttg atatggaaat ctattttttc caaccaatct aaaatcttgt aacctaactc
    23521 ttctgacatc ggaaaagagg cgttgatttg tgcattggag atatggtcaa catggatacc
    23581 ggaagtgatc gtacaacttc ccggaacggc agttttgatg atcacagcta ttttttcggc
    23641 taagacatcg tctttatgga accgaccatc gtggctaggg aaacgtacag tttgcatctc
    23701 tgtatcacga gaaaatgtag tgactgttcc gatatgtggt gtatctccgc ctgttaaagt
    23761 gatcaataag tctttaccga ttactttaac agcagcttgg atcgtgaaac ctagtttggt
    23821 tacttcaact atcgtttctt tttccatatt aatcaccctg ccaacgtttt gaatgctcat
    23881 ttacaatttc aaattgatcc aaaagtttca ttgtatggtg attaacaaga tcttggatcg
    23941 tttgaggctg attataaaaa gcaggaattg gtggtatgat ttgcacacct agtcgtgcta
    24001 atttagtcaa gttttccaag tgaatggcat ttaatggcgt ttcacgagga acaatgacta
    24061 actttcgttg ctccttcatt gttacatcag cagcacgtgc aatcaggttg tcgcctaaac
    24121 caacagagat acctgcgact gttttcatac ttgccggtac gatgatcatt ccatcggtca
    24181 aaaatgatcc gctggcgaca gcagcaccta aatctttgtt tgaataatgg taatcaaaca
    24241 ggctttcgaa ttcagttagt gataagtcgg attccagctt taagttctgt tttgcccatg
    24301 gactgatgat tccgtgtgtt tctatcatcg gatattcttt tagtttcttc actaggttga
    24361 tggcataaat ggtgcctgaa gcgccactta caccaatcac gatttttttc ttccgttgtg
    24421 ccaattgttc cattttccgc actccttttt atgatgtcaa aacagttgca gatcattcat
    24481 tatttcaaat atttttccca atcttctact tcttggaatt ttgcacgtac aaaatgttct
    24541 ttcatatcga aaggaactgt accgtcaaag atagtttttg cacttatccc acggaaacga
    24601 atagagccag gattatattc tggtaattct gatgggtcca atggatggtt cctcattcct
    24661 gggatcacga tgatatcttt atctccttgg aaacgagtgt tgaaagtcca aactacatcg
    24721 ttcatgtcga aaatatcaac atcttcatcg actaaaatga ctgtcttcaa ttctttgaag
    24781 gctgataaag caagaatagc tgcttgacgc tgaatacctt catccgcttc gttttcttta
    24841 cggatttgca tgatggtcat caatttacca ccacctgcag gaggattgta gacatttact
    24901 acttttcctg gaatagcttt atcgaccaag gcaagaatac ttgcttctgt aggaataccg
    24961 gccatagata cgtgttcttc actgggacca atcgttgttt gcatgattgg attgtctttt
    25021 cggtgagtaa ctgctttgac tttcactact tgtacagctg gattagcatc cccagtatag
    25081 ccaggaaatt ctggcattgc ttttcctgag ttcgtgttga tatcttcttg cattgtttca
    25141 tttggcatga tttctgcttc aatgatgaat tcagcacgag caatacccac ttcatcgact
    25201 gctataccat caacgatttg aataggttcg ttacgcaaag ctcctgcaca ccatagttca
    25261 ttgtagccaa gtggagtggt aggtggttcg aaagtcgtgc caatggcaat tgctggatct
    25321 aaaccaatat tgatagtgat aggcattggt ttattcaagg cttcgaattg tttttggaag
    25381 tgaccaatgt ggcgtccgcc aggcatgatg tacataccga tcgtatcttt atcttctaga
    25441 accatacgat ggatcgttac atcgctcatt gttttttctg gatctgatcc taagactaca
    25501 cccattgtga tgtatggacc agcatcttca ggagtatttg taggtgcagg caaaatgttg
    25561 cgaatatcga agtctgcatc ggtagctaga tgaacgactt cttgtgctgg agcgtctgct
    25621 ttgtctactt tcacaggttt gattggattt tctacagcgt ctttcaagaa acgaccaagt
    25681 gttcgataat catggtgcaa aatttcacct acacgtttac gactagccat tgttccgatg
    25741 ttcacacgtg tgccggggaa acctttgata ttgttgaaag tcatagttgg tccttcttga
    25801 gtcggacgtt ttactgtacc gccagcaccg atataacggt aaacaccaga taattctgct
    25861 tcaggatcga tttcaatatc tgtttcgtga tattgacctg gaagtgtttt tagttcttcg
    25921 atgacttttc ttaaatcata cggttgatct gtcatgagag attcctcctt agttgtcttt
    25981 acctatctat agtaaaaggt gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt
    26041 ctacaatgga atggatgcgt ttttttgata atagaataaa ctatgatata cttatcacta
    26101 aaagaaagcg catacttagt gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc
    26161 ctaaagcagt aaaagtggaa aatatagcga atattttgaa agttacattt gaaaacggag
    26221 aagtaaaata cgtgaaatct cattggacag aagagataac cgatgccttg caatttggaa
    26281 aaaaaggaag aggaaaaaga aaaaatcttc tcgcactttc tcgtaatatg tggattggga
    26341 ctgaagtaac gattgaagca gatggaacag tattcataaa tggaaaagac aggtatacgc
    26401 cagaagagtt gtggtacaaa ggaaagaaaa gtataccaga attataaaaa ataattaaaa
    26461 aaaacagcgc gaaacaagga acatattact tcctcgtttc ttgctgtttt ctactatttc
    26521 agcgctctta tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg
    26581 atagcggcgt cttttttcgc tgccattcgt gcaggaagat gaccgccaag catcgtcaat
    26641 atagtagaga cgatcactag aataatcgca tggacaggat ttagttgagc aacatttttt
    26701 agatccgtca tgttgtataa gatgctattg atagggaacg tagctagcca tgcaataaac
    26761 acacctaata tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg
    26821 atgtcttttt tacgtgcacc taaagctttt agtacaccga tttcttttgt ccgttcaatc
    26881 acagatgtat aagtaatgat actgatcatg atcatacttg ttactaaaga gatccctgca
    26941 aaagcaataa ggacataagt gatagcatcc atcaatccgc cagtcagttc agtcatcgtt
    27001 ccagctaaat cagtataaat gattttatct tcatctgatt tgcctttatt gtaatcatct
    27061 agataatcca agatcttttc tttgtcttca aaattgttcg gataaatcat gatacttgaa
    27121 ggcaagctgt ctccacctaa ataactaagc aatgtttgtt tggcattttc gtcaactttt
    27181 tcagtagtta agacatttac accactgtct ctttgagctt tgacgatttc tgattctttg
    27241 ttttcgttga cgatttgagt cgttaattga tcgctataag cgattcctgg agataagaga
    27301 ttcattgtag aagaagattt cacgcgtagg atccccgaga tcttcaattc atcagaagca
    27361 ttctgataca tcgcatcata atctgtatta gggataaaat tcccagttgg aagttttgta
    27421 tagtaagtat tgttgtttac taatttgaat gttgtaccaa cgatatcatc aaaatcaagt
    27481 ttttcgtctt ctttcacatc aaatccaaga ttcttcaatg cgttgatatt agtattatta
    27541 ttgccgtcta cgatcagcac gacatctgtt gctgaggctg gataacttcc ggcaagtaaa
    27601 gaataattgt cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac
    27661 actcccacac ctgtcattgc tgacatcgta ctagataaag aaagagattc cgtatcagga
    27721 ttttgattcg agaagctgac aggctgtact ttgccattta catcacgtaa aagattgatt
    27781 cctgtagtac gggtaaatcc gatattatta cttaagttag gatcgatgtc tgttacgtaa
    27841 tcgacgtatg tttgatcaat cttattcgtg tgctgtgctt tatcttcttc gctgactttt
    27901 gcagtaatcg ttttgctgtc tggataatcc gctttgcttg cacccaatcc ggcatcgtca
    27961 cgagtctggt tagtcgttac cttagaaata gtaatcggaa atttcgccat cgtttcagct
    28021 tgcgtgttat cgatttgttt ttggaatcca gaagaaaggg acagaacgat cgcgattccg
    28081 atgatcccaa tactggaagc aaaggaggtc aagaaagtac gtcctttttt tgttcggata
    28141 ttgttgaagg aaagctttaa cgctgtccaa aaactcatct ttgttctgcg taagttaaat
    28201 tgatcatctt ttggtcgttc gatgtgagga tgggaatctg ttaagatctt accatcagaa
    28261 aattcaatga ttcgatctgc gtattgatgg gccagttcag gattatgtgt gaccatgatc
    28321 actaattttt ctttcgataa ctcttggatc aattccatga tctggatact tgtttctgta
    28381 tctaacgctc cggttggttc atcgcacaaa aggatatctg ggtcattggc tagtgctcga
    28441 gcgatggcca ctcgctgcat ttgtccacca gataactgat ttggcttttt atgcatatga
    28501 tttgtcaagc cgactcggtg caatgcatct tctgcccgtt ttcgtttttc gtctttagaa
    28561 acaccactta atgtcatccc aagttcaacg ttttcaataa tgcctagatg accaatcaag
    28621 ttgtaacttt gaaaaatgaa accgatagag ttgtttcggt aggcatccca gtcactgtcc
    28681 ttaaagtctt tagtagactt tccgttgatt accatatcac cggaatcata attatctagt
    28741 ccaccaatca cattcagcat agtcgtttta ccagaaccac taggacctaa aatcgctacg
    28801 aattcttttt gtctgaaagc gacagacaca ccatccaatg ctttggtggt ggtttcgccg
    28861 actttataat atttttttat atcttttaat tgaagcataa tcacccttct cccgtattga
    28921 aatattcggt atactaataa gtatatcaga acaatgatcg tagtatacga aataaatgtt
    28981 aactgaagat gaactacaag aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa
    29041 tgaatcgtat tttagtagta gaagatgatc cgcaattaca gttgctttac cgttctgtgt
    29101 tggaacgagc gggcttttca gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac
    29161 tagaagcgac acgtatagaa ttaatcatta cagatatcat gatgccgaaa atggatggct
    29221 atgacttgct agactcatta cgtagcacca gatttgacac acctgttcta atgattacgg
    29281 caaaggccga ttttgaggat aagaaacgcg ggttcaaatt aggagcagat gattatatga
    29341 caaaacccat cgatgtcaat gagatgatcc tacgtgtgga agcattgctt cgacgttcaa
    29401 agatcaatta cagcagtagc ttgataatcg gacaaacaaa attagagcta gatgcttata
    29461 cagtgttaca agatcagcat tcgttgattc tgcctcaaaa agaatttctg cttctgtata
    29521 aattactgtc ttatcccaac aagatcttca ctcgccaaca gttgatggat gatatttggg
    29581 gattagatac gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc
    29641 gttttgaaaa gacggaagat tttcaaatca tcacagtacg gggactaggc tataaggcgg
    29701 ttatttaaag atgaaaaaaa agctattttc acaattatgg atctattttg ctagtattgt
    29761 cttcatttcg atcttagtga cactgctgtg ttttctcggt tttattttct tattgtctcg
    29821 acattctatc tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt tattcgtctt
    29881 tgcggggttc agtatggtcg tgggtacagg aatatcgatt tttgttgggc ggcgtatcct
    29941 gcatccaatc agtgcgctag ggacaaatat gagtctggta gcaacgggag atttttcgat
    30001 tcgaatggat gaacagcaga aagtagcaga agtacagcaa ctgtataaag atttcaacgt
    30061 tatggtccaa gaactcaata gtatcgagac actgaggaat gactttgtgt ccagtgtatc
    30121 ccatgaattc aaaacaccac tagcaacaat ccaaggatat gttcaattgt tacaagcccc
    30181 aaatctctct gacgaagaac gccaaatatt tttatatcgg attatcgaaa gtatcacgca
    30241 gctttcacaa ttaacagaga acatcttaaa attgaacaaa ctggaaaacc agcgtatcca
    30301 actggaaaag aaagagtatc gattagatga acaaatcaga gaagtaatcg ttttttttca
    30361 gcctaaatgg gagaaagaac agctagaatt ggatatcgaa cttgctgcag taaattacac
    30421 aggcaatgaa gagtttttgt accaagtgtg gctgaatatc atggataacg ccataaagta
    30481 caatcaaatc aatggacaga ttcatatcaa actatttgag acagccacag agattgtact
    30541 ggaagtgacc gattcaggtg taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa
    30601 attttatcaa ggagatacaa gtcgtcaaat ttctgggaat gggctaggac tttcactggt
    30661 taaaaaaatt ctagaactgc atgatggacg tattgattat agcagtatcg aaggagtagg
    30721 aacgacagca atgatacggt taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt
    30781 ctattgaagg tttttagggg tttttagtcg tttttttgtg attagggaaa tgttcttacg
    30841 gttcttttgg cataaaaatc gctgctttct tattgaacta cgagaaatgt tagaggtgtc
    30901 attgaaacaa ggacatgatg caagaaagag agcgaaagtc atgcgtcgct ctcttttttt
    30961 gggtggatag atggacagaa aatgtagggc ttagttttct tcttgattta aatactccca
    31021 aaatgtatct gaagccaaag aatgctcacc gactttacga gtaaatgcaa gataactctc
    31081 tgttgttgga gaaataggga gaatcacagt attttctttt aatagattct tgactgtttt
    31141 ttccataaca atagcaatcc ccatgtcatt tgcaatcata ttgatgatca agtcgattct
    31201 tgctccttta taagagattt ttggttcaaa tccagcttcg cgacacagat ctagtacagg
    31261 ttgatacaag ttcgtagtgg ggccaaggag caaaaagttc tcctttttca gttcgcttag
    31321 atccaatttc tttttactag ctaaaggatg atttttaggt agaactgcta caaaatcgtc
    31381 tgtttcagta ggcaaccact caatcgaact tggtggggcc tggaaacttc tagcaaagtg
    31441 gatcgtgtca ctatgttcat tggcgtcatt gtaagttaat tcgacacttt ccacttcttt
    31501 caactggact gtaaattcag gatggcgttt caaaaactcg gtgattttgg aaaaaccttt
    31561 ataattgatc attgtcggta tcgttaagat atttaatgaa agattgttgt ctttcgtata
    31621 ggtttcaagc acttcttgca tagcatggta ttgttctacg acatttttta catatgggag
    31681 caataagctt ccagcttcgg tcaattcgat ttttctatgg gcgcgttcga ataatttgac
    31741 gtctaattct gtttctaacg aaagaatctg tttagaaata tttccttggg tggtgaattg
    31801 tcgatcagca gttttggtga aattcattgt ttcggctaaa tcaagaaaaa cttcgaattt
    31861 atggatatcc atcatattcc tcctacttat tccaataaag aatactttaa cacaaaaaat
    31921 gaattggtgt aatcttctct gtctgttaca atctttcttg cagcgaaagc aataattaca
    31981 gaaatttttc tgattttttt cgcttcgtag aaaaagagag gagttttttg atgccaactg
    32041 gagttttgat caatgtaggt tccgttttgt ttggcggact aattggcgga ttagtaggaa
    32101 ataaattaag tgaacatttt aaagcacagc tgacgatggt ttttggtgtg tgttcgatgg
    32161 ggatgggaat ctattccatt gcgccgatga aaaatatgcc ggctgttatt tttgctttag
    32221 tgataggaac agcgattggt ttgattgttc atttaggtaa tgggataaat aagggggctg
    32281 cgctgatgca agtacctatt tctaaaatct tccctagtga aaagcttggt atgacccacg
    32341 atgaatttat cagcacatta gtgacggtca ttgttctatt ttgtgcaagt ggtacgggga
    32401 tctatggaag tttagattct gggatgacag gagacagtac gattttgatt tctaaatctg
    32461 tccttgattt tttcacagca gctatttttg cgtgtaattt agggtatgtc gtttctgtga
    32521 tagcaattcc ccaattcatt attttttata ttttgttctt attggcaaaa tttatttatc
    32581 cattgactac accagacatg atcttagatt ttaaagcttg cggcggattc ttgatggtcg
    32641 ctaccggttt tagaatgatc aacgtgaaaa tgttcccagt agctgacatg atacctgcaa
    32701 tgatcgttat catgccactg agctggatgt ggacaaattg gattatgcca ctgttataat
    32761 aaaaatcaaa gagaagccat tttttgctga ctaaaaagga tcaaatcttt tagtcagttt
    32821 ttttgtattt gacaaactga ttttgaaact atattatatg ttgtataata tagattgaaa
    32881 taaaatatta cacttgagaa tagaaggtga cgtaatgcac atccagattc caactgttct
    32941 tcttgatggt actgtgctag cagcattaag caaggaagat atgtatgggt atgccttaac
    33001 aaaaatcgta caagaaagtc tctctgtcag tgaatctaca ctatatccgg ttttgagaag
    33061 attgaaaaaa gaaggatatc ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg
    33121 atattactcg ttgacaccaa tgggaagtcg tcatttagaa gaaattattg atgaatggat
    33181 tgtttttcga gattctttag accggttatt ggaggagagc acaaatgaat gaatatttga
    33241 atgaagttgt ggatcatctt caagaaattc ctgaagacga tcgttttgat ttgatccagt
    33301 attatgagga atatttttta gattctggaa aaacattaga tcagatcatt gatgaatatg
    33361 gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa gaagaacaag
    33421 cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg ttaatcatta
    33481 tcggtttatt tgcttctcct gtcctcattc cactagcact ggggtttata ttggttattg
    33541 ggggactatt ggttgcttta ctagcgctca tttttagtat ctatgtttta tgtatcagta
    33601 tcttaggtgt tggcctattc atgatcatct caggaatcag tgtgtttgga caatcgattc
    33661 tgagtggatt attctttatc ggtgtcggtc tgttcgccac aggaagcgca gtatttattt
    33721 cgccactgtt gctgaaagga acaaaagggc ttttttatgt catgatgaac tttgttaaat
    33781 ggatgggacg acgttttatt acaaaaagag atctgcattc agcgggcgtt tgaaggagga
    33841 cacataaatt gagattgaaa cattttttga ttaccggtat tggtctgatg cttactggtg
    33901 gcctcctagc aggtgcaact tatacattag gcgctcaaaa gagtctgact tgggataatg
    33961 gtcctcggtt gatcgatatg gttactgaga caaaaaaaat caaggatgca aacaaaatcg
    34021 tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg attggatttt gagataaaaa
    34081 cgacttatga taagacagaa aagccagaaa taaaggagaa aaatggcgtg ttgactgtga
    34141 atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga agacacaagt gcacaaatca
    34201 ctattgttgt ccctgcagat catgaattat ctgaattgac aatcgaaggg aataatagtg
    34261 caattgatat caacgaagta tcaatcaaaa aagtgaatac taagacacat gatggctggg
    34321 tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa agatgaatta ggctcacttc
    34381 gattagatga ggccaatgtg gataacttga tcgtcacagg tgaaaacagc aacttccatc
    34441 tagaagacag tttgatcagc aaagaaggaa aagtcatcct aaacggtggc aatttatctt
    34501 tgcatgagat ggaaaacagt ggctatacta tccaaacgtc tggtgaatct tatattgaaa
    34561 aagactacca gccaataaca aatactttgt tagaacaagg acagaaaaag ctaaagatca
    34621 ctgctaaaga cacatcgatc aatattacga cggatcaaga ggacaatgaa acagattgat
    34681 cctgtactca actggatatt ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt
    34741 tttcatcgct taacgcacat aactatagta aaataagaaa gaaaatatct aaaaagaaaa
    34801 cagggtgaaa gagtatatga aggtagaaga aggtcaaaga gaaaagttaa aaacagaaat
    34861 ggaaagatta catacttata tcactcaact aagccaaacc ttctatgatc cagacaaaga
    34921 aaaagtgatg gttaattacc cgaacaattc agaaggtcgc caattagagc aagtgtacca
    34981 tgaagttttc aaacatttat tgacggtgaa gaaagagctg gattattatt ctttaccgat
    35041 tatcgataca ggaatattga agtatgatgg gaagaaggaa cgatttatct ttaaatctgt
    35101 tcgggaagat ttgcctcttt cagcagggat ggatctagag gtcttggtag aagactattt
    35161 tacagaagaa aagcactggg ttcgaacaaa attggattat ttaccgcagg cagccggagg
    35221 gacacaggca agcggttggt acatcactga agacaaagag ttagaactag aaggtgtaat
    35281 ggcgcgtatt cgaaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg
    35341 agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt
    35401 gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg tatgtggcaa
    35461 gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta
    35521 agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat
    35581 ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact
    35641 gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga
    35701 aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta
    35761 ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat
    35821 atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac
    35881 acgacggcag tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt
    35941 gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc
    36001 atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga
    36061 gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc
    36121 accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa
    36181 ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt
    36241 aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt
    36301 aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct
    36361 tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat
    36421 cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt
    36481 tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa
    36541 atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga
    36601 gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc
    36661 atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa
    36721 aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac
    36781 aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg
    36841 caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc
    36901 gcttctggtt ctggtaagtc gacacttttg aatattttat ccacattaga taaaccaaca
    36961 gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca
    37021 gattttcgtg cgaatgagat tggctttatt ttccaagatt tcaatttatt agaaagcttg
    37081 acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc
    37141 aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat
    37201 ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca
    37261 caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg
    37321 gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg
    37381 acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt
    37441 atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta
    37501 tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga aattatcact
    37561 tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc
    37621 atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa
    37681 tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat
    37741 cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta
    37801 tgcaatgttt atgatgctgg gagcaaaagg cagaaaaatc gcacagatga ttttttctga
    37861 aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac
    37921 aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc attttacacc
    37981 ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc
    38041 tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga
    38101 tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg
    38161 aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt
    38221 tggtataggg gtagccttag ttacgatcgt attaggaact tatttcattt tccattctgt
    38281 gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa
    38341 caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat
    38401 ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa
    38461 tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg
    38521 ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca
    38581 aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat
    38641 catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa
    38701 aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca
    38761 agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaatc
    38821 cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact
    38881 tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa
    38941 atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt
    39001 cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg
    39061 aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt
    39121 gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg
    39181 tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa
    39241 agatatttgg gttccgttta cgatattctt cgtattgtat ttcatctact atgtactgac
    39301 gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc
    39361 aagtaataag aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttgggcttat
    39421 taccgcagat gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt gacaatattt
    39481 ttgtcacaac ctttttttca cggtctttta ttagaatata gtaaattttt tctcgttact
    39541 cacggaataa gctgttaaga aaacggtttc catgtatgct ggaatcagtg caaatgaaag
    39601 aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa actatcactt
    39661 gattacgatc acgaatcaaa atgggacatc tttgtctgtc agtgatcttg gggcacgggt
    39721 tgttcatttt caaacaataa tcgataatga aaaacgagag ttgatcttag gttttgatga
    39781 agcaaaagaa tatctagaaa aagaccctta tatcggtgct tcaatcggac gtatggctgg
    39841 acggattacg aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc
    39901 ggacactggt cattgtttac atggtggtgc acctagcttt gaagccaagc agtggcatta
    39961 tacagtcatc aatggtgaaa acgaagcatc ggtcattttc tatcagacta gcccagatat
    40021 ggaaaacgga tttcctggaa ctttagatgt agaagtacgg tatacgctga cgaacgatga
    40081 tacatggcga gtgacggtcc aagggatcag tgaccgcaaa acagtattca atcctacaaa
    40141 tcatgtctat ttcaatttaa ccggtgatcc agcacagtca atcgacgaac atatactttg
    40201 gttgaatagc cgattatttg ctacgctcaa taaagataca acgcctacag gagaagtagt
    40261 ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa aaattaggtg aggtctttgc
    40321 cagtgatttt cgccaatcaa tgatagtggg cggcatcgat catccatttt tgttgaaaaa
    40381 acaatctggt ttactaaatg ctgcggaatt gacaagccca gatgaaaaag tatcgatcca
    40441 agtaaaaaca gatgcttcag gaatcgtgat ttttacagca aattttggag aagatggacc
    40501 ggaaatgcgc gggaataaat tagtaaatca tggcggtatc acatttgaga ctcaagaact
    40561 gccaggagcg gagcgacttg aaagttttgg agatgtcact ctggaaccta accaagtacg
    40621 cgagtttgta acggaataca aaataaatat aaagaaatag aataaattaa aaagggagtg
    40681 tgacagtgtc acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga
    40741 atgatttgtc taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag
    40801 tagttgtttc tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga
    40861 cacggaagat ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga
    40921 ttggcacgcc atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga
    40981 tttcagcgtt tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca
    41041 ggtcgtacgc ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac
    41101 gtccttccaa atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt
    41161 tgtagaaatc aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac
    41221 tttccttgta agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac
    41281 tatcttgttc aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa
    41341 aaaggttatg atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag
    41401 cttgagccat catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat
    41461 ctgcaatctt tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat
    41521 ctaatccatc taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg
    41581 ctaagtttac tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt
    41641 tatctcctgg ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt
    41701 gagtaacacc ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc tgacttactg
    41761 gaatctgcgt acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg
    41821 ctttcatata tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc
    41881 cccatacata tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat
    41941 tcgttgtatt tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag
    42001 cagaagatgg attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt
    42061 tcgttccgcc attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat
    42121 tgttattcga tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt
    42181 tattattgtt tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt
    42241 ttggcgtttc cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg
    42301 ttgaagtatc cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg
    42361 taaaggtctt tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt
    42421 gttcaccttc agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta
    42481 ccgttccact ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag
    42541 tcagttggat cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct
    42601 tttcattttc tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg
    42661 agttagttgt tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc
    42721 tggatgtagt cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata
    42781 cggtattggt cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg
    42841 tttttttatt tttcataata aactagtcct tttcctttca ttgattttct gtcttattca
    42901 gcattcatta gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa
    42961 atctgtctgt gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt
    43021 ttctttgacg ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat
    43081 aaaaatggtt atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata
    43141 aaaataatga tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt
    43201 ctttttcatt aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt
    43261 gatcattgct acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga
    43321 aatgaatgca ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc
    43381 aaatcccatt acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt
    43441 caatgggatc gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc
    43501 aaaagcagaa ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa
    43561 agctgcacca gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc
    43621 ataagcagag atattactga tcatgacagg gtttacagcc cattggatac caaatattac
    43681 caaaatattc caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat
    43741 ccaagtaatt gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat
    43801 taacgtcaaa ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt
    43861 ttcgcgaata tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat
    43921 cgccggcatg acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt
    43981 agctgctttg ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc
    44041 aatgacagcc gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa
    44101 gattggcaga aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct
    44161 tgtttcacta agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga
    44221 accggcaaac agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc
    44281 gatcggattt agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta
    44341 agcaggagcc aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata
    44401 cttaatggaa gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa
    44461 tttgatacga agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa
    44521 ctgttcaatc agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt
    44581 ttccattaga agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct
    44641 gtacgaataa atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag
    44701 ctaatcggtg tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc
    44761 tttatttctt ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa
    44821 acagataaaa aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag
    44881 gggacaggca tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt
    44941 actcgtgaaa tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat
    45001 tgttaatttt agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca
    45061 ccataagact gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag
    45121 ttactaaggt ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct
    45181 gttgtgtcaa cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga
    45241 tagaaagcgc cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt
    45301 ttttcggctg ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct
    45361 gtttctgttt catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt
    45421 tcacgagctg gtgcaggatt caagatcgtt gtttttcctg cgtcttttgc aatagaaaaa
    45481 gctaggatcg tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta
    45541 atcagttgct tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg
    45601 atcaagatac tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg
    45661 ccttggattt cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac
    45721 tgttgtcctt cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt
    45781 ttggctgcca ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca
    45841 tgcatcgttt cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata
    45901 cttccaatta ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat
    45961 gggaaaatta ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag
    46021 cacaccttga aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta
    46081 atcttttttc ttcccattct ttttctggga attggatccg atctaacaat aacttggcag
    46141 atgtttgtcc caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact
    46201 cgcacatatc tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag
    46261 cttcctctaa tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag
    46321 ttgcagcaga atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg
    46381 taggtaagag gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc
    46441 cctcaatccg agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt
    46501 gatgtcctag actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc
    46561 gtacggcatc actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt
    46621 gttttttcaa attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc
    46681 catccacacc tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat
    46741 cagcattaca taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca
    46801 tcagtgtact gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt
    46861 ccttggtaat catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag
    46921 ctaaaacttt ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg
    46981 agacagttgt gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt
    47041 tcaccatgtt catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa
    47101 gtaaatgaaa atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg
    47161 tttcgttgag cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga
    47221 ttctttgtca cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa
    47281 ggcatctaat gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt
    47341 ttcgattcca cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt
    47401 gtattttgtg atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc
    47461 agtgaattct ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata
    47521 agggaaagtg gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt
    47581 tcttactaac aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc
    47641 gttatttacg atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa
    47701 aatcatattt tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta
    47761 tgtaagtaaa tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc
    47821 tagagaaatt cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata
    47881 gcgagaaata tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat
    47941 acaaacgata caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa
    48001 ttgaaaaagg cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc
    48061 ttaatatttt aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag
    48121 atattgcgca attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg
    48181 ttttccaatt ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa
    48241 cggaagtttc tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc
    48301 atcgattgaa taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg
    48361 ctcgtgcact agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg
    48421 attttgaaac gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca
    48481 ataccgtttt gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc
    48541 acattaatga tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg
    48601 agattgtatg gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg
    48661 aaattcggca atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac
    48721 ttttatttgt aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg
    48781 ttggtacagc aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag
    48841 atattgctca agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt
    48901 attccaatac tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc
    48961 agaacaagct acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg
    49021 atgaaaaagc ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg
    49081 aattgaaaga aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca
    49141 atttggagag aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac
    49201 ctgaatccgt ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa
    49261 agaatttgaa tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg
    49321 cagaaaagta tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca
    49381 acgaagaatt agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag
    49441 caagaacacg gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc
    49501 agcttcctga agcgcaacga acaatcgctg ttcaaacatt atctcaacaa gaagctcaac
    49561 ttgctgaaca agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag
    49621 aattgaatcg aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg
    49681 aacgttcaga aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga
    49741 ttgccaatgt ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa
    49801 tgacacgtat ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata
    49861 cgaaagttga aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta
    49921 ttgttttagg aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta
    49981 acgagcggta tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag
    50041 cagctgccgc attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt
    50101 taagagaacg gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt
    50161 ttcttgaata catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc
    50221 gaaatctctt ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg
    50281 ccgggctgat ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac
    50341 aatttggaac gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata
    50401 gtcgtcaaaa agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta
    50461 ttcatactca aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga
    50521 tcgtaccaag taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga
    50581 acccacgctc cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg
    50641 acttatctac aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg
    50701 aagtaaaaga aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt
    50761 atgcttcagt tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga
    50821 tgacaacaaa agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa
    50881 atacgtcttt catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact
    50941 cggttgtctt aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc
    51001 taactaatat caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat
    51061 tttttgataa agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag
    51121 gaatcatcgg aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt
    51181 ctatggagaa tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag
    51241 gattaactat cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata
    51301 tcgatatgat tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcggt
    51361 ttgacctgag tattagagaa acaaacggca aaatagtttt tcatattttg ccgttttttg
    51421 agctaatacc gaaggacagc tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc
    51481 ctgcatcgag caaaaagaat actcaaataa aattagctcc tcatgtttta catttgagaa
    51541 gactgattgc cgtagataaa agcttttgaa caccatttat agaagaaaca agaatatttt
    51601 gctattttaa acaagaggat agacaaatac atgtctgatc tcttgttttt tattgggcaa
    51661 cagacctctt agaatgtata aagataaatt ttacaaacgc tttcggttca gttataataa
    51721 taaatgataa cttgtatata taagttgtgg taggtaataa atcagttgaa tggaggtaaa
    51781 tatccatgac atttaaagac aaagtcattt accaaatcta tcccaaatct tacaaagaca
    51841 gtaatggtga tggaattgga gatttaaaag gaatcaaaga aaaactcccg tatctgaatg
    51901 atctaggcat cgacatgatt tggctaaacc ctatttatcc aagccctcaa aaagacaacg
    51961 gttatgatat ctctgattac acagccatcg atcctatctt tggtacgatg gaggaattcg
    52021 aacagctaat agccgaagca aagaaatacc ggatagaaat catgctcgat atggtgctga
    52081 atcacgtttc gacagaacat gaatggttca aaaaagcact agcaggggac cagcattatc
    52141 aggacttctt cattttaaga gaagagccaa cggattgggt atcaaaattt ggtggaagtg
    52201 cttggtcgcc atttggagat acggggaaat attatttgca cttatatgat cggacacaag
    52261 ctgacttgaa ctggcggaac caagcagtaa gagaggaact attcaaagtt gttcgttttt
    52321 ggatggaaaa aggggtaaaa ggattccgtt tcgatgttat caatgttatc ggtaaagacg
    52381 aacaattgaa aaataataaa gaaaatgatg gcaaacctga atatacggac aaaccaatca
    52441 cccatacgta tctaaaagaa ttgaaccaag caacgtttgg gacggattcc actatcatca
    52501 cagtcggtga aatgagctct acgacgatcg aaaattgtat cttgtataca gaacctagta
    52561 gagaagaatt atcaatggtc tttaatttcc atcatttgaa agtcgattat gaaaatggga
    52621 ataaatggtc tactcctcca tttgattttg cagaattaaa gcgactattt catacatggg
    52681 gagaaaagat gagtgagggt aacggctgga atgctctatt ttttaataat catgatcagc
    52741 cacgtgcgct caatcgattt gtagctatcg atcagtatcg aaaacaagga gcagagatgc
    52801 tagcaaccat gatccatttg aaccgtggga cgccctatat ttatatggga gaagaaatcg
    52861 gcatgatcga cccagactat tcatcgattt ccgactatat tgacgtagaa agtttgaatg
    52921 cttataagtt actccttgaa caaggattca cagaaaaaga agcgtttcgt cggatcaaag
    52981 caaaatctcg cgataactct cgaacaccca tgcaatggtc agacacagaa caagcaggat
    53041 ttactacagg gaaaccttgg ttaaaagttg gaggcaagct agaagagatt aatgtagaaa
    53101 aagaactatc ttctgaagat tctattttgt cctattacaa gaaattgatt cggctgagaa
    53161 aaacatatcc aatcgttgca caaggagatt atcatgctta tgaagcaaat catccggaag
    53221 tttatggctt tcttcgccaa tttgaaggac agcagctgct tgtattaaca aatttctatg
    53281 ataaagatac aaggatcgcc atcccagaag gattcacaga agccaatatt cttatcgata
    53341 attattccga tactgttctt catcaaagtc tggagctaaa accataccag gcaattgctt
    53401 tgctgaaaaa ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc
    53461 gacgttttgt gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc
    53521 ctttatcgcc ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga
    53581 gtaatttgac taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat
    53641 cacccatgac aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca
    53701 ttactcgagg aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa
    53761 atttgacagc cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga
    53821 taaacggccc aatatatgga atgatacata acaacccagc agaaatagct aaaacactag
    53881 caaattctaa tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag
    53941 caatgatttg cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt
    54001 taaaatctgt acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag
    54061 gatctctcaa aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg
    54121 taacagttga aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa
    54181 atccagctat atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg
    54241 caaaaggtgt tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg
    54301 gcaaactact gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc
    54361 caagccccaa taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa
    54421 ggtgcttgct caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca
    54481 ataatggggg caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct
    54541 gatggaaaaa ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg
    54601 tttttcctcc taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt
    54661 ttcctccttt atctataaat tcattataga caaaagcggg tataaaagga aaatgataga
    54721 ggttgattgc aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga
    54781 aaacgcttga cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact
    54841 attacggttt gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg
    54901 tcataaatga aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact
    54961 gcttgcgggg caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc
    55021 ttttatccca tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta
    55081 ttgattccag cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag
    55141 atccaagata cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca
    55201 atccaaaaaa cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt
    55261 ttacttccgg gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat
    55321 acacatgagc tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca
    55381 aacatacgtg atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat
    55441 gcagcagcct ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag
    55501 caagcaaaac aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa
    55561 tatggattga atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg
    55621 agaattgctg aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa
    55681 gaaaacgcca aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa
    55741 gtattgaatc ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt
    55801 tctatcatgg aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta
    55861 gaagatactt tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag
    55921 gatgtacaag atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat
    55981 gtagaagatg ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag
    56041 acagcacaag aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc
    56101 aagatcagtg agaaagagat ggcatttacg tttaagaatg gaacaacgaa acgttcaaat
    56161 tatcgttatg tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat
    56221 ctattcgaag cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac
    56281 acgattgcac cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa
    56341 gtattgctga aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt
    56401 gaaattgcac aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc
    56461 ggctggtcat ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat
    56521 ccacggatag aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta
    56581 ttaatcgacc gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc
    56641 cgatgcattt tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa
    56701 tggacttcca gtttatgtgg cgctcctagc gaagtagaaa aatattggat gtctttgatc
    56761 ggtaaaaatt tgatctcatc attaaactct attttgaaga tatctttttg gatttgggac
    56821 atttgaaaat cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa
    56881 agatcttctt tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac
    56941 atttccgtat gggaagtcac atagattatt tttcctaaag gatctaattc acggatcaat
    57001 ttaccgatcg ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata
    57061 ccaaaagaag aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa
    57121 atatggacat aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa
    57181 acttctaatt gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt
    57241 tattttattt gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg
    57301 aaaagataag gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt
    57361 tttggatttg tttttgttga gaaccctttt tgtttcaact ggtttaacgg taatgtattt
    57421 gttgttgtat tgatcacacg aa
//
LOCUS       DACDEA010000008        54269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDEA010000008 DACDEA010000000
VERSION     DACDEA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.008"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(356..2737)
                     /locus_tag="IV136_000517"
     CDS             complement(356..2737)
                     /locus_tag="IV136_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8464533.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2727..3374)
                     /gene="recU"
                     /locus_tag="IV136_000518"
     CDS             complement(2727..3374)
                     /gene="recU"
                     /locus_tag="IV136_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8464534.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3438..3977
                     /locus_tag="IV136_000519"
     CDS             3438..3977
                     /locus_tag="IV136_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8464535.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4067..4486
                     /gene="gpsB"
                     /locus_tag="IV136_000520"
     CDS             4067..4486
                     /gene="gpsB"
                     /locus_tag="IV136_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8464536.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4508..4875
                     /gene="rnpB"
                     /locus_tag="IV136_000521"
     ncRNA           4508..4875
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV136_000521"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5084..6250
                     /locus_tag="IV136_000522"
     CDS             5084..6250
                     /locus_tag="IV136_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8464537.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6296..7792
                     /locus_tag="IV136_000523"
     CDS             6296..7792
                     /locus_tag="IV136_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8464538.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7873..8556
                     /locus_tag="IV136_000524"
     CDS             7873..8556
                     /locus_tag="IV136_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8464539.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9201..10391
                     /locus_tag="IV136_000525"
     CDS             9201..10391
                     /locus_tag="IV136_000525"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8464540.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10493..11446
                     /locus_tag="IV136_000526"
     CDS             10493..11446
                     /locus_tag="IV136_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8464541.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11515..12864
                     /locus_tag="IV136_000527"
     CDS             11515..12864
                     /locus_tag="IV136_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464542.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13047..13370)
                     /locus_tag="IV136_000528"
     CDS             complement(13047..13370)
                     /locus_tag="IV136_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464543.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13499..14662
                     /locus_tag="IV136_000529"
     CDS             13499..14662
                     /locus_tag="IV136_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464544.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14732..16081
                     /locus_tag="IV136_000530"
     CDS             14732..16081
                     /locus_tag="IV136_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8464545.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16126..16557)
                     /locus_tag="IV136_000531"
     CDS             complement(16126..16557)
                     /locus_tag="IV136_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464546.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16945..19587
                     /gene="alaS"
                     /locus_tag="IV136_000532"
     CDS             16945..19587
                     /gene="alaS"
                     /locus_tag="IV136_000532"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8464547.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19787..20383
                     /locus_tag="IV136_000533"
     CDS             19787..20383
                     /locus_tag="IV136_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8464548.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20505..21653
                     /locus_tag="IV136_000534"
     CDS             20505..21653
                     /locus_tag="IV136_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8464549.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21691..22104)
                     /locus_tag="IV136_000535"
     CDS             complement(21691..22104)
                     /locus_tag="IV136_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8464550.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22183..22614
                     /locus_tag="IV136_000536"
     CDS             22183..22614
                     /locus_tag="IV136_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8464551.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22713..22946)
                     /locus_tag="IV136_000537"
     CDS             complement(22713..22946)
                     /locus_tag="IV136_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8464552.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23129..24799
                     /locus_tag="IV136_000538"
     CDS             23129..24799
                     /locus_tag="IV136_000538"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8464553.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24946..25332
                     /locus_tag="IV136_000539"
     CDS             24946..25332
                     /locus_tag="IV136_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464554.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25319..26017
                     /locus_tag="IV136_000540"
     CDS             25319..26017
                     /locus_tag="IV136_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8464555.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26022..26486
                     /locus_tag="IV136_000541"
     CDS             26022..26486
                     /locus_tag="IV136_000541"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8464556.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26483..27394
                     /locus_tag="IV136_000542"
     CDS             26483..27394
                     /locus_tag="IV136_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8464557.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27629..28168
                     /gene="pyrR"
                     /locus_tag="IV136_000543"
     CDS             27629..28168
                     /gene="pyrR"
                     /locus_tag="IV136_000543"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8464558.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28182..29465
                     /locus_tag="IV136_000544"
     CDS             28182..29465
                     /locus_tag="IV136_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8464559.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29478..30404
                     /locus_tag="IV136_000545"
     CDS             29478..30404
                     /locus_tag="IV136_000545"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8464560.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30423..31703
                     /locus_tag="IV136_000546"
     CDS             30423..31703
                     /locus_tag="IV136_000546"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8464561.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31708..32784
                     /gene="carA"
                     /locus_tag="IV136_000547"
     CDS             31708..32784
                     /gene="carA"
                     /locus_tag="IV136_000547"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8464562.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32789..35974
                     /gene="carB"
                     /locus_tag="IV136_000548"
     CDS             32789..35974
                     /gene="carB"
                     /locus_tag="IV136_000548"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8464563.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36028..36804
                     /locus_tag="IV136_000549"
     CDS             36028..36804
                     /locus_tag="IV136_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8464564.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36801..37733
                     /locus_tag="IV136_000550"
     CDS             36801..37733
                     /locus_tag="IV136_000550"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8464565.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37730..38446
                     /gene="pyrF"
                     /locus_tag="IV136_000551"
     CDS             37730..38446
                     /gene="pyrF"
                     /locus_tag="IV136_000551"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8464566.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38453..39082
                     /locus_tag="IV136_000552"
     CDS             38453..39082
                     /locus_tag="IV136_000552"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8464567.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39070..39384
                     /locus_tag="IV136_000553"
     CDS             39070..39384
                     /locus_tag="IV136_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464568.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39422..40063
                     /locus_tag="IV136_000554"
     CDS             39422..40063
                     /locus_tag="IV136_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8464569.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40094..40798
                     /locus_tag="IV136_000555"
     CDS             40094..40798
                     /locus_tag="IV136_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8464570.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40795..41697
                     /locus_tag="IV136_000556"
     CDS             40795..41697
                     /locus_tag="IV136_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8464571.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41855..43561)
                     /locus_tag="IV136_000557"
     CDS             complement(41855..43561)
                     /locus_tag="IV136_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8464572.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43751..44374
                     /locus_tag="IV136_000558"
     CDS             43751..44374
                     /locus_tag="IV136_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464573.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44824..45873
                     /locus_tag="IV136_000559"
     CDS             44824..45873
                     /locus_tag="IV136_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8464574.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45870..46751
                     /locus_tag="IV136_000560"
     CDS             45870..46751
                     /locus_tag="IV136_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464575.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46754..47524
                     /locus_tag="IV136_000561"
     CDS             46754..47524
                     /locus_tag="IV136_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464576.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47803..48657
                     /gene="aroE"
                     /locus_tag="IV136_000562"
     CDS             47803..48657
                     /gene="aroE"
                     /locus_tag="IV136_000562"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8464577.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48835..49998
                     /locus_tag="IV136_000563"
     CDS             48835..49998
                     /locus_tag="IV136_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8464578.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50115..50525
                     /locus_tag="IV136_000564"
     CDS             50115..50525
                     /locus_tag="IV136_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8464579.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50699..52927
                     /gene="topB"
                     /locus_tag="IV136_000565"
     CDS             50699..52927
                     /gene="topB"
                     /locus_tag="IV136_000565"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8464580.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53065..53382
                     /locus_tag="IV136_000566"
     CDS             53065..53382
                     /locus_tag="IV136_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464581.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53542..53778
                     /locus_tag="IV136_000567"
     CDS             53542..53778
                     /locus_tag="IV136_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8464582.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53775..54174
                     /locus_tag="IV136_000568"
                     /pseudo
     CDS             53775..54174
                     /locus_tag="IV136_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        17693 a         9473 c        11721 g        15382 t
ORIGIN      
        1 aagcatacta tcagataact caaaatgaag ccttctttca aaaaattatt caaaaccata
       61 tattgtattt tttatttgat aataacaatg ggattattaa gaagtggcac taaataaaag
      121 actagtgtgt atttagccga tttaattata aacaagtaaa tggataaagt atatgtaatg
      181 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
      241 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
      301 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
      361 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
      421 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
      481 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
      541 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
      601 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
      661 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
      721 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
      781 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
      841 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
      901 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
      961 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
     1021 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
     1081 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     1141 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     1201 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     1261 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     1321 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     1381 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     1441 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     1501 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     1561 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     1621 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     1681 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     1741 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     1801 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     1861 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     1921 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     1981 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     2041 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
     2101 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
     2161 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
     2221 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
     2281 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
     2341 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
     2401 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
     2461 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
     2521 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
     2581 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
     2641 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
     2701 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
     2761 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
     2821 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
     2881 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
     2941 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
     3001 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
     3061 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
     3121 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
     3181 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
     3241 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
     3301 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
     3361 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
     3421 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
     3481 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
     3541 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
     3601 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
     3661 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
     3721 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
     3781 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
     3841 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
     3901 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
     3961 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
     4021 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
     4081 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
     4141 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaaactt acagcaaaga
     4201 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
     4261 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
     4321 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
     4381 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
     4441 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
     4501 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
     4561 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
     4621 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
     4681 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
     4741 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
     4801 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
     4861 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
     4921 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
     4981 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
     5041 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
     5101 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
     5161 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
     5221 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
     5281 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
     5341 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
     5401 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
     5461 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
     5521 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
     5581 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
     5641 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
     5701 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
     5761 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
     5821 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
     5881 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
     5941 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
     6001 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
     6061 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
     6121 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
     6181 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
     6241 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
     6301 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
     6361 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
     6421 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
     6481 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
     6541 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
     6601 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
     6661 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
     6721 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
     6781 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
     6841 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
     6901 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
     6961 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
     7021 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
     7081 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
     7141 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
     7201 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
     7261 aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata caaaatcaag
     7321 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
     7381 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
     7441 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
     7501 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
     7561 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
     7621 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
     7681 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
     7741 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
     7801 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
     7861 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
     7921 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
     7981 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
     8041 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
     8101 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
     8161 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
     8221 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
     8281 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
     8341 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
     8401 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
     8461 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
     8521 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
     8581 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
     8641 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
     8701 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttattcg
     8761 gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc aacaagtaaa
     8821 acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa gatggaaatt
     8881 ttatgtattt ttattggtct atgcaataaa taaattaaat attcagaata caattgattt
     8941 taacgaaagg gcttctatca atggttgtgt tgtatttatc tgcccaggta gagtaaaata
     9001 gatcattatc taaaaggtaa ttgttcatac tatataagtc atttgaaagt aaaaagatac
     9061 tgtaaataaa aaatgtggtt aaataattct cataatcagt gtaagcgtta tcaaaaatat
     9121 agcatacttg ctttgtagat gaatgacacg atatatgttt ggtatgttct gcaagacaga
     9181 tgaatatgga ggtggagaca atgtcttcca aagaactaac aatgtattta gagaaagaat
     9241 tggatcaact gaaagaaaag gggttataca atacgatcga catactagaa agtgaaaatg
     9301 gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct agcatccaac aattatcttg
     9361 gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga agcaaccgaa acttatggtg
     9421 taggagcagg agcagtccga acgatcaatg gttcgctgaa aatccatcag caattagaag
     9481 aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc ctttcaatct ggattcaact
     9541 gcaatatggg cgctatctct gcagtaatga caaaagaaga tgctatctta tcggatgaat
     9601 taaaccatgc atctattatt gacggttgcc gcttatccgg agcaaaaatc atccgaatta
     9661 aacatcaaga tatgaaagac cttgagaaaa aagcaaagga agcgatagaa agtaagaaat
     9721 ataaaaaaat catgtatatc acagatggtg ttttctccat ggatggagac attgcacgat
     9781 tgccggaaat tattccgatt gttgaaaaat atggactgat cacctacgtc gatgatgcgc
     9841 acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa gcattttgga ttaagcgaca
     9901 aaatcgatat gcaaatgggc acgttatcga aagctatagg tgttgttggg ggttatgtag
     9961 ctggatcgaa aacactaatc gactggttga aagctagaag tcggccattc ctattttcta
    10021 cttcgctaac tccaggtgca gctgcagctg cattggcttc gattactttg atgcaagaac
    10081 atccggaact tgtcgaaaaa gtttgggaaa atgctaatta ttttaaagaa gaattgaaaa
    10141 aagtaggcta taacatcggt atgtctgaaa cacctattac tccagtcatt ttgggagacg
    10201 aaaaagtgac tcagactttc tctaagaagc tgatcgaaca tgggatttat gctaaaccaa
    10261 tcgtttatcc aactgtgcct cttgggacag gcaggatcag aaatatgccg actgcggaac
    10321 atacgaggga aatgcttgat gaagcagtag cagtttatca aaaaatagga aaagaaatgg
    10381 aaattattta actaaattta gttttttcgt ctatttaaaa ttagagaaca tgttcacgca
    10441 tatattgtta atcatctgaa gatagtagta aaaaagatca gggaggatca tcatgaaaaa
    10501 agtattggtg acaggatgtt taggacaaat tggttcagag ttgatcacac ggctacgtca
    10561 agaactaggc agtggaaatg tcattgcgac agatatacgc atgcctgaag tcattgagaa
    10621 aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca gcatagcaga
    10681 acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg ttgcagaagc
    10741 aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat tggaagtcgc
    10801 tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg gtccaaatac
    10861 tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt atggggtaac
    10921 gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg tagatactcg
    10981 tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg gtacgacaga
    11041 ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta gtttcattga
    11101 tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca ttataaaact
    11161 catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat cggcgatgtc
    11221 ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt ttgaaatgga
    11281 ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt cactagatgt
    11341 ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg tgatgacaaa
    11401 ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca agaaccacat
    11461 ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca agggatggaa
    11521 gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa acgtacgatg
    11581 ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg ggtatttttt
    11641 aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt ttcttggttt
    11701 ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc agctgctatt
    11761 ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac agcagctttt
    11821 ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc cttgtcgatc
    11881 attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat tgttcccgta
    11941 gctgttacag ctgctgtatc gatcctgttg atcaattttt taggttctaa agctggaggg
    12001 gcttttcagt cgattacgct tgtctgcaag cttattcctt tgtttgtgat cgtcattttc
    12061 ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc aggagaaaat
    12121 ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc atacgacggt
    12181 tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga tttgccaaaa
    12241 gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa tgcggtgttc
    12301 ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag tgacgttgca
    12361 aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt gatttctgtt
    12421 tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt tatggcaaaa
    12481 gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac taaagttcct
    12541 gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat cggtggtttt
    12601 gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat ggtctttgtt
    12661 ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa agttccaatg
    12721 tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc aagcacgctt
    12781 atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg tatcccgatt
    12841 tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac tgcacttctc
    12901 tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca aaagtactcg
    12961 ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt aaaagacaga
    13021 aatcgggctc aaccgatttc tgtcttttac tttgatttgc gtaactgaac tattcggcgt
    13081 acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg aagccaaatc
    13141 agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt atccatttca
    13201 agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga aataatcagg
    13261 agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat cgtagcataa
    13321 aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat ctcgacaccc
    13381 cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct cgttacttgc
    13441 ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga ggagtcatat
    13501 ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt tagccttagc
    13561 gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc tagtaacaag
    13621 ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt tggtgatctt
    13681 accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg agctgcattt
    13741 atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg aacctagtac
    13801 ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg ttttattact
    13861 cataccattt ggcatttatt taagatatta tttccggctt tcttttatga aaacgatgat
    13921 tgctagtttc tgcctttctc tattttttga attgacgcag ctaagtggac tttattttat
    13981 ctacccaaga ccataccgct tatttgatgt gaatgattta gtgacaaata cattgggtgg
    14041 aatcattggt tttgtcgtta cgccgttatt tacatttatg cttcctacaa gaagacgaat
    14101 ggatgaactt tcttatgaaa aaggaaagac tgtctcattg actcgacgct tggtcgctta
    14161 tttgatcgac tggacatttt tatctcttat taatttggta gcggcgattt tctttaaaat
    14221 tgcgttcgat tcaggggcag ttacttctca tatctggtgg cttttattag aagtaggggt
    14281 atactttatg attgtaccgt ttataaccaa tggatggaca cttggcaaaa aaattgtgcg
    14341 tatccgtttg gtggaagaag accgcgaacg aatttcttta aaagcattga tgatccgcta
    14401 cgggtatctg tactttcttt tttatggttt aagctggttg gggcagttgc ttcagcaaat
    14461 catgaattcg acaacgaata cggtgttgca gatgagtgca gtattcattt ctctcggtgt
    14521 cttgttacta caaggtattt ttattttaaa tatcttgcta tcatttttga gaaaaaaacg
    14581 gattctgttt tatgaaaaag ctagtcacac atatactgtt agcacgattg gccaaacaaa
    14641 ggatgatgat tctcgaaatt agctgagcaa aaacgctttg tctgtgttat aatgccaaag
    14701 tcgtgataat ttaaagagaa gaggtgcacg aatgaataat tttgatatat tccgcttcaa
    14761 accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa cagcccacag aggtccagga
    14821 acgtttgatt cctttaattg caaaaggaaa aaatatcatt gggcaatctc aaactggtac
    14881 aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa atcaatgcag aaaaagagga
    14941 agtacaagtt gttattacag ctccaagtag ggaactagct gcacagatct atgaagcagc
    15001 tacacaaatc gctaaatttt ctacaccgga gatccgtgtc tcgaattttg tcggcggtac
    15061 agacaaacag cgtcaattaa atcgtctgca tcaccaacag ccacaggttg tagtaggcac
    15121 gcctggtaga atattagaca tgatgaatga acaagcgctg aagattcaca ccgcatttgc
    15181 atttgttgtc gatgaagcgg atatgacatt ggatatgggt tttttaaatg aagtagacca
    15241 aattgcaagt cgattaccag aaaagctgca gtttttagtt ttttctgcca ctattccaga
    15301 aaaacttcgt ccattcttac gaaaatacat ggaaaatcca atcattgaag aaatcaaacc
    15361 aaaagcagtt atttctgaag acattgaaaa ttggctgatt tcaacaaaag gtaaagatga
    15421 gaatcaaatg atttatcaac tgctgacaat tggtcatcct tatttagcaa tcgtatttgc
    15481 caatacaaaa cagcatgtgg atgaaattgc cgattacttg aaaaaccaag gcttgaaagt
    15541 agcaaagatt catggagaca tcaccccaag agagagaaaa cgagtgatgc gacaagtaca
    15601 gaatctggag tatcaatatg ttgtagcgac tgatttagct gctagaggga tcgatatcga
    15661 aggtgtctct catgttatta atgcagaaat tccagaagat ctagacttct ttatccatcg
    15721 tgtcggacgt actggacgta atggcttaaa aggaacggcc attacgttat attcgccaaa
    15781 tgatgagcaa gccattgatg cgatagaaaa attgggcgtc tcatttgaac caaaagaaat
    15841 caaaaacggg gaaatcgtca aaacatacga tcgtaataga cgtgccaaac gagaaaaatc
    15901 aaaagatgta ttagatccca cgctgattgg tcttgtgaag aagaaaaaga aaaagatcaa
    15961 gcctggttat aagaaaaaaa tcgattgggc aattgcagaa aaaaataaaa aagaacgcaa
    16021 aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat aagaaaaatt cacataaata
    16081 aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct tgtttttaag ctagactttt
    16141 tttcattccg atacaaagta cttcatcttc aataaaaggt tctgtgcaag tatgataccc
    16201 ttgtttttca taaaaatcta cagccgtcat ttcagctact acataactag tctgacaacc
    16261 ttctgaaacc gcccgttttt cagcagcttc aagcaactga ccgcctaacc cttttcctcg
    16321 gtattctttt gctacgcaaa atcgttccgg cttgaagcaa gtaggtgttt tcttttcata
    16381 acgcaaagta gctactggtc ttttttgatc aagaaataga taataggttt gttgcgggat
    16441 atcttttccg tcgaattcta gttctgcagg aatcccttgt tctaatacaa aaacttgata
    16501 tctcaaaaag cagctagctg cgcgtatcca ttcatccgct ccaactattt ttttcatcat
    16561 attcctcctt gtcatgaata gcagagatgt attgattcct ttattcttat cgtaccaaaa
    16621 atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg agatattgac agtgatcaat
    16681 agaatgcgta tactaaataa gtacgaaaag gatatgattt gttttttaag actcacagaa
    16741 aattctttct tggctgaaaa aagaatagtc actaatcaaa ttgctccctt tttctatttt
    16801 ggtagaaata ccgacgcagc tcagcgttat cgagtttaag aggtaatgat tacacatcat
    16861 tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt gatggtgttt ttttgtattt
    16921 aatttataga aagcaggtaa tgacatgaaa caattaagta gtgcacaagt tcgacaaatg
    16981 tttcttgatt tcttccaatc taaagaacat acgattgaac ctagtgcgtc tttgatcccc
    17041 gtgaacgatc caaccttatt gtggatcaac tctggtgttg caacattgaa aaaatatttt
    17101 gatggttcag ttgtgccaga aaatccaaga atcacaaatg cccaaaaatc tattcgaaca
    17161 aatgatatcg aaaatgtcgg taaaacagca cggcaccata cgatgtttga gatgctgggt
    17221 aatttttcga ttggtgacta tttcaaaaat gaagccatcc actgggcatg ggaatttttg
    17281 actgatccaa aatggatggc attcgatcca gaaaaacttt atgtaacagt ttatccaaaa
    17341 gatacagaag caaaacgcat ctggcatgaa gaagttggcc tgccactaga tcacattatt
    17401 gatgtagaag acaacttctg ggatatcggt gcaggcccat gtggtccgga tacggaaatt
    17461 ttctatgacc gtggagaagc ttataacgat ttagcagaag acgatcctga aaactatcca
    17521 ggaggagaaa atgaacgtta tttagaaatc tggaacttag tattttcaga attcaaccat
    17581 aagcctgata acacatttga accacttcct cataaaaaca ttgatacggg gatgggattg
    17641 gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact ttgagacaga cttgtttatg
    17701 cctattatcc acgccgtaga aaagctaagc aaacaagtaa aatatggtga aaacccagta
    17761 acagatattt cttacaaagt aatcgcagat catattcgcg ccctttcatt tgctattggc
    17821 gacggtgcat tgccatcgaa tgaagggaga gggtatgtct tacgccgttt gcttcgtcgt
    17881 gctgtcatgc atgggaaaaa attaggtatt gaacaagcat tcttatatcg tttagtccct
    17941 gttgttgggg aaatcatggt aagttattat cctgaagtac ttcaacaaaa agaatttatt
    18001 gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa caatcaatga aggtttggaa
    18061 atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg ggacgactct agcaggaaaa
    18121 gacatcttca aactatacga cacatatggc ttcccggtag aattaacgga agaggtggca
    18181 gaagatgaag gactgaaagt tgatcatgct ggttttgaaa aagaaatgga tgcacaacgc
    18241 gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg gggtacaatc tgctttattg
    18301 acagatatca aagtagaaag taaattcatt ggatacacag aacaagaatc agatagtcaa
    18361 ttattggtca tcgttaaagg tgagtctctt ttgaatgaag tcgcagaagg tgaagctcaa
    18421 atgatctttg atcaaacacc tttctatgca gaaatgggtg ggcaagtagc ggatcaagga
    18481 tggatcatga atgaagtagg cgaaatcgta gcagttgtta cagatgttca aaaagcgcct
    18541 aatggtcaat ttatgcacac cgtagaagta aaagcaccaa tgagtgaagg acaaacctat
    18601 catctattga tcgatcaatc gatgcgcaat cggatcatca aaaaccatac agcaacccat
    18661 ctactgcaca aggcattgaa agaagtactt ggcgagcatg caaatcaagc tggttcttta
    18721 gtcgctcctg gacatttacg attcgacttt acccactttg gtcaaatcac ttcagatgaa
    18781 ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg aagctatccc agttgaaaca
    18841 attgaaacag atatcgatac agccaaaaac atgggtgcaa tggctttgtt tggtgaaaaa
    18901 tacggacatg acgttcgagt agtaaatatt ggcggctggt caatcgaact gtgcggaggc
    18961 acacatgtag caaatacaga aaacatcgga atcttcaaaa tcgtttctga atcaggaatt
    19021 ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag aagcgtacga actacttgaa
    19081 gaagaagaaa aacaactaaa acaagtagca acaattgtta aatcacctca attgaaagaa
    19141 gttgtttcaa aagcggaaca attgcaacag caagtaagag agctacaaaa ggaaaatgaa
    19201 caattagcta gcaagttagc gaatcaacaa gctggtgatg tattcaaaga tccagaaacg
    19261 atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga aagatatgaa tcagctgcgt
    19321 caattagctg accaatggaa acaaaaaaat atttcagata ttcttgtttt agcaaacgaa
    19381 caagaaggaa aagtcagtct gctagcggca atgacgaaag aatcaaatga agctggacta
    19441 aaagctggcg acttgattaa agcaatcgca ccgaaagtag gcggtggcgg tggtggacgt
    19501 ccagacatgg cacaagctgg cgggaaaaat cctgctggta ttccagatgc acttaaagct
    19561 gcgaaagaat gggttgcgca acaatagggc gtttacgttt gacagaaaaa caaaagttca
    19621 aacattgaac gaaacatgat tatgggggcg aagacaaaca agtcttgccc tcatactttt
    19681 ttcaattgga tggcgcatct aatcaaatgg tcggattcgg atggtcttta gttgacgaaa
    19741 gtgacagaaa tttttacgat tagtataaat aaaaaggggt gaaaaaatga atagtagaaa
    19801 taagaccttt cgactagtct tacgtgcgat cttgttagca atcatcatcg tacaagcgat
    19861 ggttccttgg ctggggttta ttccattagg attcattagt ttaacaatta tccatatcac
    19921 agtaattatt gctgcagtag tattaggccc aaaagatggc atggtgattg gcttgttttg
    19981 gggaatcgct acaattgttc gtgcctatgc catgccaaca acgccatttg atacattagt
    20041 gttcacgaat ccgattatct cggtagttcc cagagtactt gttggcttag tagcaggctt
    20101 ggttttccac tggatttatc aaaaaaataa atcaataacg atcagctcag cctttgcagg
    20161 ggtactgggt tcattggtca atacagtctt agtgttagga ttcatgggat tattttatac
    20221 aggtgcaacg gcagatgcct atggtgtaga ctctgctctt ttatttaaaa ccttagcagg
    20281 ggttgcagca atcaatggga taccagaagc aataggtgcg ggaatcataa caccattact
    20341 agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa taatgaccat cttttaatag
    20401 taataggacg cacgatagat agaaaaagga agagaggaga ggaaaacaat cgtcctctcc
    20461 atctttttac ctgagcaaca agcaaactag aaggggtaaa aaacttggct aagaaacaag
    20521 aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa aggagtacta gcgggtatct
    20581 taggattggt tacgaatctg atcttgtttg tctctaagct aatgatcggg cttttttcag
    20641 gaagtgtctc cattatggct gatgcagtga acagtctatc agatactgct tcatccatat
    20701 tgactttgat cggatttaaa attgctgcaa agccagcaga tcaggaacat ccttatggac
    20761 acgaacgctt cgaatatatc agtgggttat ttgtttcgat catcattact tatgtaggat
    20821 ttcaattttt agagtcttct gctgaaaaaa tcatccatcc agaaaatgtc tctttaacac
    20881 ccatcgtctt ttttgtccta atcatttcca tctttttgaa atttgcacaa ggcaaaatgt
    20941 atcaggttat tgcgcaatcc attgattctg aaacattgag agctacagca acagatagtt
    21001 ataatgatgt ctttactaca ctggctgtat tgctttcagc agcaatcgaa tgggtaactg
    21061 gatggcgtat tgatggatat ataggttttg ctttagcatt atatatcctt tatagtggat
    21121 tgacgatgct tcgaaatttt gtatacgaac tattaggaag tcgcccaacg ccagcagaga
    21181 ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat acttggctat catgatttat
    21241 tggtgcataa ctatggaccc aataaacgct ttgcatcagt ccacatcgaa gtagatgaat
    21301 caatgaattt gaatgtggct cataagatta ttgatctgat agaaaaagac tttaaaaagt
    21361 ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc tgttgataac gaaaaatact
    21421 ataatatctt gcggcagtta aagcaaattc ttcttgaaac aggtacaggg cttaatattc
    21481 atgattttag agtaattcat caaggaaaaa cgctgcaatt tgatgtagtg gtccctgaga
    21541 aattccagtt tgttgataca gaactagaaa gaatcttacg tagtcaaata gaagaaaaaa
    21601 tcggagatta ccaattggaa attacgtttg atcacaatta tcttttatat taaatgagaa
    21661 actataaaaa gactccttaa agtttttaat ttaaagagtc tttttttctt ttaattgttt
    21721 atggagagcc tgacgtgcaa gattatctgc tcctttattt ttgttttcgg gcaaccattg
    21781 caggatcaat aaagaaaatt cggatagaag ttcttggatt ctttgaaggt agggaaggaa
    21841 gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact agtgttttgc tgtctgaaaa
    21901 acagaaaatc gtttcattct gtaagtcgtt tttctttaga aattctaaag tttttaaaaa
    21961 gacttcgaac tctgcttgat ggtttgtccc tatagaaagc gggatagata gctgttcttg
    22021 tttaccttga tagacaatca agattccacc tccactagga ccagggttgc cttttgtaga
    22081 ggcatcaatg tatactttta acataaaaag agattctcct ttttgttcaa actaatgtta
    22141 tggtaagatt atatcattac tgaatcggtg agaggttaga gaatgaaaaa aaatattcga
    22201 tggcagcctg aactggcaca tacgatcatc tattggtcat tgaccttgat cattctgttt
    22261 tttagtctga ttctttcttt agaaaacaca ggtccttatt ggaaaagcaa ttttgtttta
    22321 ggtatttttc ttgtttttct atttctaggt ttgcggcgtt cttttatttt aaaatcaaac
    22381 tctgtgatta tacgttatgc tgctttttgg aaagaccgag aaatatctgt cggacagatt
    22441 cgatctttga cgccaactaa tactggggtg caaatgatgt tgcaagatga gaaagcccca
    22501 tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt ttgatcattt tgaaaaaaac
    22561 ggacccaaat cagaatcaat tccttatcct tatacgaaaa aaagaaaaag ctaattttaa
    22621 aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa aagagtgaga cgtacggttc
    22681 atcccgttta gcgtctcact ctatttgttt atctattcat gcatactagt atcggtagtc
    22741 tgaacgttgc ctttcttgac atgggcagct tgtgttcctc gattcccttc gatgatctca
    22801 aattcgacca cttgattttc ttccaaagtt ttgaatccct cttcttcaat cgcagtaaaa
    22861 tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc cgtatccttt tttgttgtca
    22921 aaccatttta caattcctgt ggtcaaagca ctcacgacct ttctaaagtc tgatcatagc
    22981 cttcattata aagagtattt ataagtacgt caaataaatt aaaaacctgt aattttatgt
    23041 tttttttctg aataaaaaaa ggtataattg gcatatctta acaaagaaga tattttgttg
    23101 aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat ctgcaaattt cacaagaaac
    23161 agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag acgatttgga
    23221 actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat caaaggataa
    23281 cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg gcgaaggaaa
    23341 gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa aatctgttat
    23401 tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg ctgcaggcgg
    23461 aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg gtgacatgca
    23521 tgctatcaca acagctaata atgcattatc tgctctttta gataatcata ttcatcaagg
    23581 aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg atctcaatga
    23641 tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg ttccaagaga
    23701 agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc ttgcggcaga
    23761 ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat ttgaccgtca
    23821 gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc tgaaagatgc
    23881 aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc acgggggacc
    23941 gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag cattgagatt
    24001 agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag agaaattttt
    24061 agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg tcgccactgt
    24121 ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg agaatcttga
    24181 tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg aacaatatca
    24241 gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg aactcacact
    24301 gttggagcac ttgtgtgaaa atcaggggat tcttgccaag agagcgagtg tatgggcaga
    24361 tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg atagaaaaga
    24421 agctaattac aagccattat accgcctaga agaaacgata caagaaaaga cagaaacaat
    24481 cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc aaaaacaaat
    24541 agaagagttt acgaagaatg gatgggacaa actgccgatt tgtatggcaa aaacacagta
    24601 ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca tcacgatccg
    24661 cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag atgttatgac
    24721 gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa atgacggaga
    24781 agtgttggga ttgttttaag cttgattata atctaccatc gaaggagctg tattttgact
    24841 acagcttctt tttttagacg atagggaatt aattttgttt acactaggat cattcttttt
    24901 ctttattgta ttttgagcta tactaaagca taataggagg gtatgatgaa aacgacattt
    24961 ataagaaaaa ccggttttta cggtatgggt agctcgattc agctaagaat caacaatgaa
    25021 aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg atctggaagt gccctttacg
    25081 atgcaggtca ccttttattg gctaaaaagc cccgtttata cagtgaaaga agcaaagcct
    25141 gcttatgtga tcacaatgaa ttacttgatt cttcaaatct atccgttttt atttctagca
    25201 actgggttgg gggccgtttt cctacaaaat ttatttttca gtttttttgc agttattgtg
    25261 ttgattgcgt tctttttcta tataaaaaac cgggcatatt tgatcaagga ggaaaattat
    25321 ggcaaactct gatctttttt taatcagttt caatcgaatc gaaaaatgga tgagagacga
    25381 gatgggaaat gcacgaaata tgggatttac cgaacttgtc agacgcctag ctcaaagaaa
    25441 acaattaatg atcagaaaat acgaagatga tcttttacag cttgctcagt taaggaatgc
    25501 gatcgtccat gatcggatcg cagtcgattt tgtcattgca gagccaaatg attggacagt
    25561 caagcgaatc cagctgatcg aacaagaact gattcagcct gaaaccgtgc tgcctcgatt
    25621 tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta cttgaactgt taaagatagt
    25681 agcagaaaaa aggtattcac aatttccgtt atacaataaa ggaaaatttg tagcattgat
    25741 tactttacgt aatatcgggt tttggctagc aaaagaaagc caaaaaggtc cggttgattt
    25801 aacaaataaa cgagcgttgg atttgatcat ccaaaacgga aagtacacaa attatcattt
    25861 tgttccagct aaaacacata tttacgaggt tgaagcgatg tttcgtgagc aagggacttt
    25921 ggaagccgta cttatcacta aagatgggaa tcccgatggt aatctgctcg ggatcgtacg
    25981 acctcgtgat atttataaac aaatagaaaa ggattgattt tttgttaatt atttattgga
    26041 tattcagcgc tttgctagta ggagtggatc agttggtcaa atggtggatc actgataatc
    26101 tagcgcttgg agagacgaaa aatcttatac caggtatttt gtcactgaat catattcgaa
    26161 acactggagc cgcatggagt atgctagaag ggaagatgtg gttctttact atagtgacac
    26221 tcattgcagt cgttgtgatt ctcacattga tgattaaaaa tcgtgaaaaa ggtaatcgct
    26281 ggttcatgat tggattgagt ttgattctag ctggtgcgat tggcaatttt atcgaccgta
    26341 ttcgtcttgg ctatgtagta gatatgttcc agacagattt tatgagcttt ccgattttta
    26401 acgtagcaga tgtgacactt gtatgtggag tgatttgtat gttaatttat attattttag
    26461 atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg acgattcacc atgaatctgg
    26521 taggatcgat aaagtattga ctaatttatt caccgattac agtcgttcgc aaattcagct
    26581 gtggctgaaa aatggagcgg tttctgtcaa tggacagcca gtcaaagcaa attacaaagt
    26641 aaaacaagga gacgagatta ctgtcttgat ccccgaacca gaaacattag atgtggtccc
    26701 agaaaatatc ccgttgacga tcgtttacga agatgaagat gtagcggtga taaacaaacc
    26761 tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat ggcacaatgg tcaatgcgct
    26821 gatgtaccat atcaaggatc tttctacgat caacggcgtt attcgtccag gtattgttca
    26881 ccgaatcgac aaagatactt ccggactgct aatggttgcc aaaaatgacc aggcacacga
    26941 atcattagct cagcaattaa aagataagac ctctttgcgc aaatatgttg ctttagtcca
    27001 tggagagatt cctcacgaaa aaggaaccat caatgcgcca atcggccgtt cgaaggaaaa
    27061 ccggaaaatg caggcgataa gagaagatgg gaagtcggct gtcacacatt ttactgtatt
    27121 ggaacgtttc gaaggattta ctctggttga attaaaacta gaaaccggaa gaacacacca
    27181 gatccgtgtg catatgaagt atatcggtta tccgttagct ggagatccag tatacggacc
    27241 gaagaaaact ttgaaaggca atggtcagtt tctccatgcg caaatgttag gttttacgca
    27301 cccgactaca aacgaaaaga tgatctttga agcacctctt ccagaagttt ttgaaaaaac
    27361 tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga taacggtata gtaattaaca
    27421 acgaaagcat aaaacttaat agaattcctt taagtttagt cccgtgaggc taagaagggt
    27481 gggagcaagg atacgtatgg tgcgccataa gtagattgct cattgacata gtatagctat
    27541 cagtgtatca gtatacgctc agtctaaaca ttcctcctgt cctcttaggg caggagtttt
    27601 tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa gaagtagtag atcaggtaac
    27661 catgaagcga gcattgactc gaattactta tgagattatc gagagaaacc atagtatcca
    27721 agatatcgta ttggtaggta tcaaaacacg aggaatctac attgcatcaa gaattgccga
    27781 acgcctgaag cagctagaag atatcgatat tcctgtagga gaactggata tcactttata
    27841 ccgagatgac aaaaaagaaa atccagaaga accggaactt cattcttctg acatcccagt
    27901 ttccttagaa ggaaaagaag ttattttgat tgatgatgtt ttgtatacag gacgaacgat
    27961 tcgagcagca atggacgcag taatggattt tggcagacca agaaaaattt cattagcagt
    28021 cctggtcgat cgtgggcatc gagaattgcc aatccgtgca gattatgttg ggaagaacat
    28081 tccaacatct cgggcggaag agattttagt agagatgcaa gagcttgatg gacaagatcg
    28141 catcatgatt ttgaaagagg aagattagag gagagagaaa catgtcggag aaaaaaggat
    28201 ttcataatga tgacgtggtg ttagatatcc acgatcgccc aaccccagga cattgggtag
    28261 gattaagttt acaacacttg ttcacgatgt tcggagcaac cgtgttagtc cccatcttag
    28321 taggtatcga tccaggtatt gcgctagtca gttcaggact aggtacattg gtgtacttat
    28381 ttactactaa aggaaagata cctgcgtact taggaagcag cttcgctttc attgcagcga
    28441 tgcagctttt gatgaaaaca gatggttatc cagcgattgc acaaggagca gtcacaactg
    28501 gattagtcta tttgattgtt gcgctgatca tcaaaaagat cggttcagca tggctggata
    28561 aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt tatcgggttg ggacttgcgg
    28621 caaatgcagc aacaaatgct atgtataatc agggcacgta tgatttcaag tatattgctg
    28681 tagcgctgat cacattagga ttaacaattt tcttcaacat gtggctaaga ggttttctcg
    28741 gattgattcc gattttacta ggtattatta gcggatacat cgtagccttg ctgtttggaa
    28801 tcgtggatac taagccaatc gtagacgctg cctggtttgc tgttcctaac tttgagattc
    28861 cctttgtaca atatgaacca aagctatatc ttggagcaat cacaacaatg gcacccatcg
    28921 cttttgtgac gatgacagag catatcggcc atcttatggt attgaataaa ctaactaaac
    28981 gtaatttctt tgaagaaccg ggtcttcata agacgttgac aggtgatgga ttagcacaaa
    29041 tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta cggtgaaaac attggtgttc
    29101 tggctatcac acgtgtccat agcgtgtttg tgatcggtgg agctgcagta tttgcagtca
    29161 ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag tatccctgga ccagtcatct
    29221 caggaatcag ttttatctta tttggagtaa ttgccgcaag tggaatgaag attttaatcg
    29281 agaacaaaat cgattttgat aaaaagaaaa atctgttgat tgcttcagtt atcttagtag
    29341 taggaattgg cggactcgtc tttgaagtag gcacatttac attgtcggca atggcattag
    29401 caactgtact tggcatcatt ttaaatctta tcttaccaga aacttcacga agcgaagaaa
    29461 actaaggagg aaacattatg atcatcaaat cagaacgtat cagcttaaag catttattga
    29521 ccgttgaggc acttactgat caagaagtta tgggcttgat ccgcagaggg caggaattca
    29581 agcaaggtgc taattggact ccgcaaaagc gccagtactt tgctacaaac ctctttttcg
    29641 aaaatagtac tcgcacacat aaaagcttcg atgtagcgga gaaaaaacta gggctagaag
    29701 tgattgaatt tgaagcaagc acaagctctg tccaaaaagg ggaaacacta tacgatacag
    29761 tactgacgat gtcagcatta ggcgtagacg tagcggttat ccgtcatggt gatgagaact
    29821 attatgacga actgatacaa agtaaaacaa tccagtgttc gattatcaat ggaggagacg
    29881 gaagcggaca gcatccaacc caatgcttgc ttgatttgat gaccatttat gaagaatttg
    29941 gtcattttga aggtctgaaa gttgccatcg taggtgatat cactcactct cgcgtagcta
    30001 aatccaatat gcagatgtta aaacgtctag gcgcacaagt attcttctca ggtcctagag
    30061 aatggtacga cgatgaattc gaagtgtacg gacattatat gccattggat gaactactag
    30121 atcaagttga tgtcatgatg atgcttcggg tgcaacacga acgccatgat ggaaaagaaa
    30181 gtttctcaaa agaaggctac catcaggaac atgggttaac agttgagcgt gcgaagaagt
    30241 tgcaaaaaca tgcaatcatt atgcacccag caccagttaa ccgtgatgta gaattagctg
    30301 attcattagt ggaaggatta cagtcgcgga tcgttcagca aatgagcaat ggagtattcg
    30361 tcagaatggc aatcttagaa gcagtgctaa atggaaaagc gtaattatag gaggatgaaa
    30421 aaatgaaaac actcattaaa aacggtcaga tcaatacacg aaaaaatatg acgacaccag
    30481 cagaaatttg gatagaagat gggcgaatca aagcaatcgg tacaggattc tcagaagccg
    30541 aatttgatga agtgtttgat gcaaagggac aattgatcac acctggttta gtggacgtcc
    30601 atgttcattt gagagagcca ggatttacat acaaggagac gatcgaagct gggactcgct
    30661 cagctgctcg tggcggtttc actactgtat gcgcgatgcc gaatttaaat cctgtacctg
    30721 atacggcaga aaagctgaga caagtatatg acatcattag aaaagatgca gttgtgaagg
    30781 tattgcagta tgcgccaatc acagaaaatc tgcgaagtga gaaattagtt gatcaagaag
    30841 cactgattga agaaggtgct tttgctttca caaatgacgg tgtaggtgtc caaacagctg
    30901 ggacgatgta tctggctatg aaagaagctg caaaaaacaa taaagcccta gtagcccata
    30961 cagaagatga atctttatta tttggcggcg ttatgcacgc tggcaagaag gcagaagaac
    31021 ttggtcttcc cgggatctta agcgtaaccg agtcttctca aattgctaga gatctgttgc
    31081 tagctgaagc aactggtgtt cattaccatg tttgccatgt ttctactaaa gaaagtgtcc
    31141 gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac agcagaagtt tcaccccatc
    31201 atcttatttt gatcgacgaa gatattcccg aagatttcgg cttttggaaa atgaacccgc
    31261 cgctaagagg acgggaggac agagaagctt tgatcgaagg cttgcttgac ggtacgatcg
    31321 attgcatcgc tacagaccat gccccccatg gattagaaga aaaaagccaa agtttcatga
    31381 aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca actgatctat actcattttg
    31441 tagaaactgg tcgattcacg ttggaacaag tgatcaattg gttggcagtt aaaccggctg
    31501 agatttttgg tttgaatgca ggaacgctaa cggttggtgc ccccgctgat gtagcagttt
    31561 ttgatattac acaaacttgc acgatcgata aagaagactt tttatcaaaa ggggagaata
    31621 caccttttat cggctggaaa gtaaaaggcg aaacacaaat gacttttgtc aacggaaaac
    31681 ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc ttatattaga agatggaaca
    31741 gttttcgaag gaaaggcttt tggtgcagat gctagtgtct tcggagaaat cgtttttaca
    31801 acaagtatga ctggttatca agaaactatc actgatcaaa gttttaacgg tcaaatcatt
    31861 acatttacct atcctatggt gggtaattat ggggtaaata gagatgatta tgaatcgatt
    31921 tctccaacat gcaaaggagt agtagtaaaa gaacatgcac gtgtagcttc aaactggcga
    31981 aaccaaatga cgctagattc atttttgaaa caaaaaggaa ttcctggaat atcaggtatc
    32041 gatacgcgcg cccttactcg caaaatccgt gaagtcggaa cgatgaaagc tagtatagtt
    32101 gatgctggag attccttcga acatgcattt gatcagctga aagcaaccgt tctagcaacc
    32161 aatcaagtgc agcaagtctc aactaacaaa ccttatccaa gtccaggaac aggtcggaaa
    32221 gtcattgtca ttgacttcgg cttgaaacat agtattttac gagaattgag taaaagaaac
    32281 tgtaacctca cagtacttcc ctataacaca gatgccgaga cgattttagc gctatcacca
    32341 gacggagtca tgctgacgaa tggacctggt gatccaaaag atgtgccaga agcaatcgaa
    32401 atgatcaaaa aaatccaagg aaaagtaccg atttttggta tttgtctcgg tcaccagtta
    32461 tttgctttag ccaacggtgc agatacctat aaaatgaaat tcggacatcg aggattaaat
    32521 cacccggtac gagaagtagc gaccggaaga attgatttta cttctcaaaa tcatggctat
    32581 gcagtcgatg aagccacgat tgatccagaa aaactcatcg taactcatgt agaagtaaat
    32641 gacggaacag tagaaggagt caaacacaga gattatcccg ctttcagtgt gcagtttcat
    32701 ccagatgctg cccctggtcc gcatgatgca ctacatttat ttgatgaatt tatggaaatg
    32761 atggacgcag gaaaggagca gtaaacagat gccgaaacgt acagatatca agaaaattat
    32821 ggtgattgga tctggtccga tcatcattgg tcaagccgca gaatttgact acgcaggaac
    32881 acaagcctgc cttgccttaa aagaagaagg atatgaagtg gttttagtaa attctaatcc
    32941 tgcgacgatc atgacggata aagaagtggc agatcaagta tatatcgaac cgatcacact
    33001 tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat gctctccttc caactttagg
    33061 cggacagaca ggcctcaata tggcaatgga gctggcagca tcaggtattt tagaagaatt
    33121 gaacatagaa cttctaggaa caaagctttc tgcgatcgat caagcagaag atcgtgatct
    33181 attcaaacag ttgatgaaag aattgaatca gccaatcccc gagtctgaaa tcgtcaatac
    33241 agtagaagaa gctgtagatt ttgcaaataa aatcggctat cctgtcatcg ttcgtccagc
    33301 ctttacttta ggcggaactg gtggaggtat gtgtgaaaat gaagaagaat tacgtatcgt
    33361 atcagaaaat ggattaagtc tttcccccgc tacccaatgt ttgattgaac gaagtattgc
    33421 aggttttaaa gaaattgaat atgaagtgat gagagattcg gctgataatg caatcgttgt
    33481 ctgcaatatg gaaaattttg acccggtggg tatccataca ggtgattcta tcgtatttgc
    33541 ccctagtcaa acattgtctg accatgaata tcaaatgctt cgtgatgctt ctctttcgat
    33601 tattcgagca cttaagatcg agggaggctg taatgtccaa ctcgctcttg atccacatag
    33661 ttttaattat tatgtgattg aagtcaatcc gcgtgtttct cgttcatctg cgttagcaag
    33721 taaggcgact ggctatccga tcgctaagct agctgctaaa atagcgcttg gattaacatt
    33781 agacgaaatg aaaaatcctg ttacaggaac gacttttgca gaatttgaac ctgcattaga
    33841 ttatgttgta gctaaaattc ctcgctggcc gtttgataag tttgaaaacg gagaacggct
    33901 tttaggcacg cagatgaaag caactggtga agtgatggct atcggtcgga atatcgaaga
    33961 gtctttattg aaagctgtgc gttctttaga aatcggtaca caccatatcg aacttcctga
    34021 gttggcagac atcacggacg atgacctaat ccaaaaagtg gttcgagcac aagatgatcg
    34081 cctgttttat gtagctgaag ctattagaag agggtacacc atagaagaat tagcggattt
    34141 gacaaaaatc gatttattct ttttagataa actgttgcat atcgtagaaa tcgaacagca
    34201 gttgaaacag cagccgttgg atgacaagat attgaaaaca gcgaaacaaa acggttttac
    34261 agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa gccgttcgcc agatgagaaa
    34321 agaaaatgat ttgattcctg tatataagat ggttgacact tgtgcagcgg aattcgaatc
    34381 aaaaacacct tatttttata gttcctatga atatgaaaat gaaagttcac gatcagaaaa
    34441 gccctcaatt ctagtactgg gatctggtcc tatccgtatc ggacaagggg ttgaatttga
    34501 ctatgcgact gtgcattctg tcaaagcaat ccagcaggca ggatacgaag caatcatcat
    34561 gaacagcaat cctgaaacag tttcgactga cttttctatt tcggataaat tatattttga
    34621 gccattgacc ttcgaagatg tgatgaatgt gatcgagcta gaacagccga taggagtaat
    34681 cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa cctttgactg ctgctggtgt
    34741 aaaaatctta gggacaacga tcgaagactt ggatcgtgcg gaaaatcgtg atttattcga
    34801 gcaagcgctg aaagagttgg atattcctca acctttaggg gatactgcta caagtaaaga
    34861 agaagccgtg gttatcgctt cgagaattgg ttatccagtt cttgttcgtc caagctatgt
    34921 attaggcgga cgtgcgatgg aaatcgttga aaaccagcga gatttagaag attacatgga
    34981 acatgctgtg aaagcatctc cagaacatcc agtactagtg gaccgttact tgcttggtgg
    35041 tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta ctcatcccag ggatcatgga
    35101 gcatattgag cgtgctggtg tccattcagg ggattcaatg gctgtgtatc cacctcaaac
    35161 attatctgaa tcgatcaaga aaacgatcga agattacacg atccgcttag cattaggttt
    35221 gaattgtgtc gggatgatga atatccagtt tgtcatccat gaaaatgagg tttatgtgat
    35281 cgaagtcaat cctcgagcaa gtcggactgt tccattctta agtaaggtga caggaattcc
    35341 aatggcacaa attgcgacga aagctatttt gggagagaaa ctaaagacac ttggttatga
    35401 aaacggactt tatccagaaa caaaagaagt tcatgttaaa gcacctgtct tttcttttac
    35461 gaaattgcag aaagttgata cttacttagg gcctgaaatg aaatcgactg gagaagtaat
    35521 gggatctgac agacaattag acaaagcttt gtacaaggca ttcgaagctt ctggattaca
    35581 cttgcctgaa tatggagccg tattatttac gattgcggac gaaacaaaag aagaagcact
    35641 agaattagct aaacgtttta cagaaattgg ctatagtctg ataggaacga aacatacagc
    35701 tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta gcgaaaattt ctgaggaagc
    35761 agcagaaaaa aatgtagtcg acttaataag agatggtaaa acacaagtgg ttgtcaatac
    35821 gattgataaa gatcggggaa atgcttctaa agacggtttc atcatccgaa gagaagcagt
    35881 tgaacatggt attccattgt ttacttcttt agacacagca gatgcgatcg ttcgagtgat
    35941 ggaatcccgt gctttttcaa ctaaccctat ttaacggcga gcacatgtta agacaaaaag
    36001 acgaaaaacc gttgcaggag gggacagatg aaacaagaaa tgatggaaat cgttcagcaa
    36061 aaacagctgg caccaaagat ttttgaaatg acattgaaag gtgatctagt aaaagaaatg
    36121 cagcaaccag gacagttctt gcatattctc gtgccgcgtg cagacttgct attacgccga
    36181 ccgattagct tgaatcatat cgaccacgaa acaaatactt gccgtatcat ttatcgggtg
    36241 gaaggtgagg ggactagagt cttctctact ctttctgccg gagaccattt agatgtcatg
    36301 ggtcctttag gcaatggttt tgatttgtcc aatcttcata caggagacga agtatttata
    36361 atcggtggag gaatcggtgt ccctcctcta tatgagttat caaaacagtt agtcgaaaaa
    36421 ggaattaagc caacgcattt tttaggtttt gccacacatg aagtcgttta ttatgaaaaa
    36481 gaatttaatc gtctaggaga aacaagaatc gctactgacg atggaacata cggtatgcac
    36541 ggaaatgtcg gtaatttatt gttaggaacg aagacggaac ctacagctgt ttttgcatgt
    36601 ggttcaaacg ggttattgaa gacagtggaa cagctgtttt cttcgcatca aaatgtccag
    36661 ttatcattag aatcccgcat ggcatgtggg ataggggctt gctatgcttg cgtttgtcat
    36721 acgccggaag atgaaacagg aacaaaaagt gtgaaagttt gtgacgaagg accgattttt
    36781 aaaataggag aggtaattat atgaatgaga atcctttagc ggtcaaactt cctggcctag
    36841 aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg ttttggcaaa gaatacggaa
    36901 aatactatga tttgaatcag cttggatcga tcatggtcaa ggcaacgact ccaaatgccc
    36961 gctttggcaa tcccacgcca agagtggcgg aaacaccatc tggcatgttg aatgcaattg
    37021 gtttgcaaaa tcctggtcta gacgttgtta tgcataaact tcttccagaa ttagaggagt
    37081 acaatgagtt acccatcatc gcaaatgttg ctggtgcttg tgaagaagac tatgtagaag
    37141 tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat cgagctgaac atttcttgcc
    37201 caaatgtcaa acatggcgga attgcttttg gaacagattc tgatgttgct tttcaattaa
    37261 cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt caaattgtcc ccaaatgtaa
    37321 cagacatcgt tccaatcgct caggcaatcg aagctggagg ggcagatggg tttacgatga
    37381 tcaatactct tcttggtatg cggattgacc ttaagacaag aaaaccgatt ttagccaatc
    37441 aaacaggtgg actctccgga ccagcaatca agcctgtcgc tattcgtttg attcaccaag
    37501 tagcaagtat atccagcctt ccaatcattg ggatgggcgg cgtgcagaca gtggatgatg
    37561 ttcttgagat gtttatggct ggagcaagtg ctgtagctat agggaccgcg aatttcaccg
    37621 atccgtatat ctgtcctaaa ctaattaaag agctccctgt gcgaatgagc gaactaggca
    37681 tcgaatcgct ggaaagatta atcaaagaag tgagagagga acgtgaacga tgaatcaact
    37741 agaccaacga ccaattattg cattagattt ttcaactagg caagaggtag aagactttct
    37801 tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc ggaatggaat tgttctacca
    37861 agaaggacct gaaatcgtac gttatctaaa agaggctggt cataatgtat ttttagatct
    37921 gaaacttcac gatattccga atacggtcga aaaagcaatg cggggattgg caaagcttgg
    37981 tgttgatctc acctgtgttc atgccgctgg cggtattcgt atgatggagg cggcaatgcg
    38041 aggattagaa gaggggactc cagaaggtgg aaaacgtccg ctacttctag ctatcactca
    38101 gcttacttct acaagtgaag aagaaatgca tgccgatcaa ctaatcgaag tttctttaga
    38161 aaaaagtgtg atccattatg ctagctgtgc caaaaaagca ggattagatg gtgtggtttc
    38221 ttctgcttgg gaagtagaag cgatcaaaga aatggcagga gacgaatttg tttgcttaac
    38281 tccgggtata agaccagaag gaacagtcgc aggagatcag acaagagtag tcacaccttc
    38341 tcaggcaaaa aagatcggct caacttttat cgttataggt cgaccaatca ctcagtcaac
    38401 taatccttat aaagcatatc aaacgattca aacagaatgg agctaaccta aaatgaatgt
    38461 tgaacaaagt atcgcaaaag acttattaga aattgaagca gtatttttaa atccaagtaa
    38521 tccttttaca tgggcaagtg gaatcaaaag cccaatttac tgtgataacc gaatcacgat
    38581 gagctatcca aaagtccgaa aagagatcgc aaaaggatta gcaagcaaaa tcaaagaagc
    38641 ttaccctgaa gtacaagtga ttgctggaac tgcaacagca ggaatccctc atgccgcatg
    38701 ggtagcggaa atactggatt tgccaatggt ctatattcga agtaaggcga aagatcatgg
    38761 gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag aagatggttg tgatcgagga
    38821 tttgatttct acaggcggaa gtgtcttgga agcagctgaa gcagcaaaaa gagaaggtgc
    38881 ggatatcctt ggtgttgcgg ctatctttac gtatgagttg ccaaaaggaa aagcaaattt
    38941 cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat tctgtattga ttgaagcagc
    39001 tttagaagat cgttatatcg atgagcaaga attaactctg ctaaaagaat ggaaaaaaga
    39061 tcctgaaaat tggcagattt aagcgcgctt gatgaagata gagaaatccg atttgtgtct
    39121 ccagaatcat caattggaat agcaaaaatc caaaaactga ttgcgagaag catcttttca
    39181 tcaggtatga aaagaaactt ctatcgtgat cagtttttga tttttttaaa attcattttt
    39241 ctaagactgg ttagcagaaa gaagagaata atctcttctc ttttttctat tatatataga
    39301 ttttctctat tttttggtaa gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg
    39361 aatatcagtt atttaatgat atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt
    39421 tatggggttg aatgaattta ttttaggatt gaacaattta gagacgatca caagggcacc
    39481 tggatttttt aaatatacag agcatacagt tgcagcacat tcgtatcgtg tagcatctat
    39541 tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct
    39601 ctatgaaaaa tcattgaatc atgactacac agagcgtttt attggtgata tcaaaacgcc
    39661 ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa acagttgaag aaaagatgac
    39721 ggacgaattc atcaaccaag agattccagc tgagttccaa gaagtttatc gccgtcggtt
    39781 gttcgaagga aaagatgaaa ccgtagaagg agaactatta gccatttcag ataaggttga
    39841 ccttctttat gaatcttttg atgaaatcat caaaaataac ccggaaaaag tttataagga
    39901 gatgttcatt gaggcagtga ctacgattaa aagctttgaa catcggcctt ctgttcagta
    39961 tttctttaaa gagatatttc cggaactatt gaaccaggaa ttttatggaa aagaggcgtt
    40021 tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca
    40081 gggggaagta tagttgaaaa agaatatgga tgtcgaatgg ggctattctg gagacaaagg
    40141 tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga ggtgcggaat ttccattgca
    40201 gtcgcctatt catttgacca agggggaaga aaccaaaata gaaggagaca cgctttcctt
    40261 tcattatcaa aagcaacggt ttactgacaa ggaattcaaa aacacgattc atttagtacc
    40321 ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc tattatttga cagatattca
    40381 tttccacttg ccttctgaac atatcatcaa cggaaaacag gaagatattg aatttcattt
    40441 tgttcatatg aacagcaaaa aagagaatct agttgtgggt gtcatgtacc agctaatggt
    40501 acaggaaggt tggctgtata atcaagaaaa tggggaaagt tggaacttag aaaaacatga
    40561 gcattgggta aatccggatg tgttctttcc ggagcaaaaa agccatttcc attatattgg
    40621 ctcattgaca actccgccta ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg
    40681 aaaactgaac caagagtttc ttctcccctt cgatggtcag tatgctcgac cgaacaaccg
    40741 accaatccag cccttgaatg gaagagaaat ttattatttc gaggagtttg agcaatgaat
    40801 attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg gtagtttcag agaagcggca
    40861 aagaaaatgt ttgtctccca gccaagtctt tcagctggta tcaaagaatt agaaaccgag
    40921 ttagggatca cgctgtttac tcgaacgaat cgaggggcat atttaacaga agaagggcaa
    40981 gacttcttaa atcgtgccga gaaaatattg gtccagcttg atacattaga aactcactat
    41041 ctaacgagtg aaaagagaga caatttttcg atcgctgccc agcattatga tttcttaggt
    41101 cctttgaccg cacagctact cgaggaattc aaagacgaga tcaaacaatt tcggatcatg
    41161 gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc agcacagtga gatcggcatt
    41221 atttatatga atgagcataa tcaagctggt atcagaagat atttacaaca aggagagcta
    41281 agtggccgca gcattggaaa attcaaaacg catatttttt taaggcaagg acatccatta
    41341 gctgaacacg cagagatcac caaagaagaa ttgttatctt atcctcaagt tcgttttaca
    41401 caggatggaa ataatttccc gtatttttat gaagacctta tcgagatccc tgatcaagaa
    41461 acagtgattt atacgagtga tcgaggtact ttgatgaata tcgttcttga aactgatgct
    41521 tacgcttcag gatctggtat tgtgattgga gggattcgcg agcacttgcg gttaatccct
    41581 ttggcagaag gcgaactaaa tgaattttat atcatttatt cggcgaaacg tccgctcagt
    41641 gaaatcgctc aaagatttat caaagagctt actcggttac tggaccagca gagctaagaa
    41701 aagcagatga gaacgctgtt tattgaacac cgttgatcaa aaaagagggg ttgtgacaga
    41761 attttgtcac aactcctctt tttgtagcaa acaatttata actaattgct gttgtgggaa
    41821 agtacgcaaa tatgattcta gaaacactca ttgattacct gttttgtttc atagctgtaa
    41881 ttttttcttc ttctggtgtc acgataatcg tcttttggtt ttcataaata acataaccag
    41941 gcttggcgcc atttggtttt cggatatgct ttacctgtac aagatcaacc ggcacttggg
    42001 cagagtatcg gtattttgaa aaataggcag ctaactcggc agcttctgta attgtttcat
    42061 ctgagggctt gtcactttta atgatgacgt gcgaaccggg tatatttttt gcatgaagcc
    42121 aatagtcggt ttttttagct gtcttcattg tcagctggtc attttgaaga ttgtttttac
    42181 caacaagaat gagcgttccg tcagaagaaa agtattgatc aggctgagat ggtttcttac
    42241 gtttttgttt ttttaagttc ttctttttca ggtatccttc tgaagtcaat tcttctttaa
    42301 ttgcttcgat atccattggg ccggcaattt ccagttgaga taagacagat tcaagatatt
    42361 ggatttcatc tttggcttct tggatttgtt cgccaatcag cttaacagcg ttcttcagct
    42421 tttggtatcg atggaaatat ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg
    42481 ctattttgat tggccgatct tcttcataat aattaggtaa aacaacttct tttgcgccgc
    42541 gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt tctacggtaa ttctcagcgt
    42601 tttcggattc tttgagtgtt tgttcacgtt ttttcaattt atttttattt cgttttagtt
    42661 cattttcgat ttttcgaatc agttctccgc cttgttgttt tgcccgatct ttctctgctt
    42721 tttcgtgata atagctatct aataattgac tgagtgttgg ataagctgtt acagcagatg
    42781 cctgttcgga caatacctga taagcaatcg gtgtgaaaaa atctttattt tccgtttcgt
    42841 aaaaagtagg aataggctga tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct
    42901 tttcattagg acgttccagt aatcttttgg tcagctcttg agcgatatcg aaaccgattc
    42961 cctgaaactg acgttggatt gcttttggat ctagttcagt ttgggaaagc cgctgaaaga
    43021 ttttttcttt ctcggagcta aatgggttca agacctcttg ttttggcgga gcaacatatt
    43081 ctacaccagg aagcaaagaa cgataagtgt tttgagagct tccaatgtgt ttgatcgcat
    43141 ccagtatttt tccagtttct cgattaacta agacgatcgt actatgacgt cccattaatt
    43201 caacgatcaa tatgatattt tgtagatcac ccaactcgtt tcgtttagca aagtggaaat
    43261 ggatcactcg atcattttcg atttgttcga tgtcttctaa aatagcgcca tccaaatatt
    43321 ttcgaagcat catcacgaag ttaggcggat tatcaggatt ctgataatcg atttgggtaa
    43381 tctgtacacg agcataactt gggtgcgcag ataataataa tcgatggttt ttgccgcgtg
    43441 aacgaatcac cagcacgatt tcattttcat aaggctgatg gattttcgag atccggccag
    43501 atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca
    43561 tggttcattc ctcatttctt ttcataagta tattcattat aacgaaaaaa agaagaaaaa
    43621 ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc ttggactaat tacataaaaa
    43681 acagtgtttc tatcgttgaa atccaaattt ttaggtataa taaaaaatag gagaaaaagt
    43741 agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag aaatgaatga
    43801 acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc ttgtttttct
    43861 gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa aaatccgaga
    43921 agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga aaccttttga
    43981 ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga tgttcagcaa
    44041 accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg agcttatgga
    44101 agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg aactggaaca
    44161 aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag gaaaagaagc
    44221 aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt tgatccctga
    44281 actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg atagaacggg
    44341 agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga aacggtgggt
    44401 ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa aataacaaat
    44461 aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta aatcaattct
    44521 taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa
    44581 attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat agatacaact
    44641 ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga ctgccgcata
    44701 gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt
    44761 ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg caggaggaaa
    44821 aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta tttagcaggt
    44881 gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa
    44941 aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga tgagatttat
    45001 cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc
    45061 aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca acagctagta
    45121 caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc attagcaaac
    45181 acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg ggctggatta
    45241 gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca gccgcctgtt
    45301 acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg
    45361 tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga agctgcaaaa
    45421 aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg
    45481 gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa taccattgcc
    45541 aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt cattccatct
    45601 gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca atatcaatta
    45661 gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa accggaaacg
    45721 acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca agctgatcgc
    45781 ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac agatgactcg
    45841 cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca caaggcatgc
    45901 tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac tttcctgata
    45961 tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct attactcatg
    46021 gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata gcaggactcg
    46081 ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg attttagtga
    46141 tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt tctttattga
    46201 atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac tttgacacag
    46261 tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc ttcttcaata
    46321 cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct cggtcgttag
    46381 ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt gtcattgcat
    46441 tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa gggattggcg
    46501 taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa gaattgacgc
    46561 tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta ttgattttag
    46621 tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct atcatcttag
    46681 ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg ggttttgaga
    46741 aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa
    46801 tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca gagaagggga
    46861 cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca atacgatcgc
    46921 tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga tcacaaaaga
    46981 ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca agatgggaac
    47041 ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag gtcaaaaacg
    47101 gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac tatgtcaaga
    47161 agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat ctggtggtca
    47221 acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac tattattaga
    47281 tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat taacaaatca
    47341 gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg aagacgcctt
    47401 gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag atatttctga
    47461 aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag aagtagtgga
    47521 ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt gacatcttcc
    47581 tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg ttttgtagga
    47641 tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac aagtaaacga
    47701 tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt atcaaacgct
    47761 aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc tggacaaacc
    47821 aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt gatgcacaat
    47881 actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt aaatccaatg
    47941 aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt aaatctttcg
    48001 atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga agcgcaattg
    48061 atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta taatactgat
    48121 ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg
    48181 actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagcagc gttagacggc
    48241 gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca
    48301 ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt tgccgatacg
    48361 catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac agatatcggg
    48421 atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca tgagaatcta
    48481 gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa
    48541 gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc aatcgcgttt
    48601 gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt tgattaaaaa
    48661 aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa gtaatccttt
    48721 atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg ataggctttt
    48781 gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac cacaatgagt
    48841 cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg ggatacgggg
    48901 agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga agatgctacc
    48961 cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga gcaacagata
    49021 tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt caatcggcaa
    49081 aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg
    49141 attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa atatgtagct
    49201 gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca aaaagcgacg
    49261 aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga tgccgggcga
    49321 gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa actgatttct
    49381 tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat caattcagct
    49441 tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt tggaatcgat
    49501 tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc
    49561 atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt agatattaaa
    49621 aaagggggcc agaagatgga tggacatgtt cttttgcagt acgcacgttt ccgaatggat
    49681 gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc tattttcagc
    49741 caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa agctttagga
    49801 tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc gattgcacga
    49861 ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa
    49921 agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc tgctattaca
    49981 gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca
    50041 tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt taaccaagag
    50101 aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt gaagccatcg
    50161 ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac ggtgaaatgc
    50221 ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc
    50281 tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt ggtcctgaat
    50341 tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa gaaacgaagc
    50401 gcctatttga aagcttagct gagggtggta agattgagat gccactggat gctgttccgt
    50461 gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag attaaccaag
    50521 agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga tcatcgtttg
    50581 tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta ttgccagatg
    50641 cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag gtcagaagat
    50701 gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca
    50761 acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg ctggtgaagt
    50821 aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag cttatgaaga
    50881 gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct tccgttacga
    50941 agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc agtcagccga
    51001 taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat
    51061 tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga tcaattctct
    51121 tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga actattttcc
    51181 aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa tgaatggaag
    51241 tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt ctcttggtcg
    51301 tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca aacacttcaa
    51361 aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc agttttctgg
    51421 aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg aaatagaaaa
    51481 acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga aaaaggaaaa
    51541 gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga tgaatcagct
    51601 aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg aaagcaagtt
    51661 tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc agtatctggt
    51721 taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga caactgagat
    51781 caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg caattattcc
    51841 gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac agcaagcaat
    51901 ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata tctacgaaga
    51961 gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa agatcccatt
    52021 acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag ctcagactct
    52081 tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg agaaagaaac
    52141 acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa
    52201 aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga
    52261 agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg aagtcaataa
    52321 aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag ccaatgaacc
    52381 attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa
    52441 acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc tacatttatt
    52501 ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta gcgggacacg
    52561 tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa aatgtaaaaa
    52621 gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa
    52681 attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag atttgctttc
    52741 aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc
    52801 agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga tcgaaaacag
    52861 tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt
    52921 agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag aatataaata
    52981 ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg ctgattttat
    53041 atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa ttatcttatg
    53101 ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga ttacgggacg
    53161 aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat tttatcgact
    53221 ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag catgctaaag
    53281 cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat tatttatttt
    53341 ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga gagtttgtga
    53401 caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa gcaaaataaa
    53461 agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat agaatgagaa
    53521 tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc
    53581 aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt
    53641 gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa agcacaagaa
    53701 gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga cgcgttaaaa
    53761 gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag
    53821 ttattttacc gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa tgcattagat
    53881 atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc tgaaatctat
    53941 gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca taatggcaat
    54001 aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg tccagttgct
    54061 ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc attggatttc
    54121 gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa atgaacgaat
    54181 taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg atttttttat
    54241 gtttaatcat acaaaatagt tttttcata
//
LOCUS       DACDEA010000009        51846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDEA010000009 DACDEA010000000
VERSION     DACDEA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.009"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..580
                     /locus_tag="IV136_000569"
     CDS             2..580
                     /locus_tag="IV136_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464583.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(820..903)
                     /locus_tag="IV136_000570"
     tRNA            complement(820..903)
                     /locus_tag="IV136_000570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(867..869),aa:Leu,seq:gag)
     gene            1286..2503
                     /locus_tag="IV136_000571"
     CDS             1286..2503
                     /locus_tag="IV136_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8464584.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2687..3523
                     /locus_tag="IV136_000572"
     CDS             2687..3523
                     /locus_tag="IV136_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8464585.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3599..4747
                     /gene="nagA"
                     /locus_tag="IV136_000573"
     CDS             3599..4747
                     /gene="nagA"
                     /locus_tag="IV136_000573"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8464586.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4968..6026
                     /locus_tag="IV136_000574"
     CDS             4968..6026
                     /locus_tag="IV136_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8464587.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6045..7064
                     /locus_tag="IV136_000575"
     CDS             6045..7064
                     /locus_tag="IV136_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8464588.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7166..7882)
                     /locus_tag="IV136_000576"
     CDS             complement(7166..7882)
                     /locus_tag="IV136_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8464589.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7851..8636)
                     /locus_tag="IV136_000577"
     CDS             complement(7851..8636)
                     /locus_tag="IV136_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8464590.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8627..9376)
                     /locus_tag="IV136_000578"
     CDS             complement(8627..9376)
                     /locus_tag="IV136_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8464591.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9674..10198
                     /locus_tag="IV136_000579"
     CDS             9674..10198
                     /locus_tag="IV136_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464592.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10214..11713
                     /locus_tag="IV136_000580"
     CDS             10214..11713
                     /locus_tag="IV136_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8464593.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11801..12403)
                     /locus_tag="IV136_000581"
     CDS             complement(11801..12403)
                     /locus_tag="IV136_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8464594.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12507..13463
                     /locus_tag="IV136_000582"
     CDS             12507..13463
                     /locus_tag="IV136_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8464595.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13768..14724)
                     /locus_tag="IV136_000583"
     CDS             complement(13768..14724)
                     /locus_tag="IV136_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8464596.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14898..15458
                     /locus_tag="IV136_000584"
     CDS             14898..15458
                     /locus_tag="IV136_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464597.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15451..16276
                     /locus_tag="IV136_000585"
                     /pseudo
     CDS             15451..16276
                     /locus_tag="IV136_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16286..16903
                     /locus_tag="IV136_000586"
     CDS             16286..16903
                     /locus_tag="IV136_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8464598.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16921..17604
                     /locus_tag="IV136_000587"
     CDS             16921..17604
                     /locus_tag="IV136_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8464599.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18287..19570
                     /locus_tag="IV136_000588"
     CDS             18287..19570
                     /locus_tag="IV136_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464600.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19586..20473
                     /locus_tag="IV136_000589"
     CDS             19586..20473
                     /locus_tag="IV136_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8464601.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20490..21326
                     /locus_tag="IV136_000590"
     CDS             20490..21326
                     /locus_tag="IV136_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8464602.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21329..21952
                     /locus_tag="IV136_000591"
     CDS             21329..21952
                     /locus_tag="IV136_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464603.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21949..23241
                     /locus_tag="IV136_000592"
     CDS             21949..23241
                     /locus_tag="IV136_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8464604.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23600..23697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23813..24394
                     /locus_tag="IV136_000593"
     CDS             23813..24394
                     /locus_tag="IV136_000593"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8464605.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24397..25704
                     /locus_tag="IV136_000594"
     CDS             24397..25704
                     /locus_tag="IV136_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8464606.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26120..26608
                     /gene="purE"
                     /locus_tag="IV136_000595"
     CDS             26120..26608
                     /gene="purE"
                     /locus_tag="IV136_000595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8464607.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26592..27725
                     /gene="purK"
                     /locus_tag="IV136_000596"
     CDS             26592..27725
                     /gene="purK"
                     /locus_tag="IV136_000596"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8464608.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27738..29033
                     /gene="purB"
                     /locus_tag="IV136_000597"
     CDS             27738..29033
                     /gene="purB"
                     /locus_tag="IV136_000597"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8464609.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29213..29590)
                     /locus_tag="IV136_000598"
     CDS             complement(29213..29590)
                     /locus_tag="IV136_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464610.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29846..30574
                     /locus_tag="IV136_000599"
     CDS             29846..30574
                     /locus_tag="IV136_000599"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8464611.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30574..30828
                     /gene="purS"
                     /locus_tag="IV136_000600"
     CDS             30574..30828
                     /gene="purS"
                     /locus_tag="IV136_000600"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8464612.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30830..31501
                     /gene="purQ"
                     /locus_tag="IV136_000601"
     CDS             30830..31501
                     /gene="purQ"
                     /locus_tag="IV136_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8464613.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31502..33724
                     /gene="purL"
                     /locus_tag="IV136_000602"
     CDS             31502..33724
                     /gene="purL"
                     /locus_tag="IV136_000602"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8464614.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33709..35148
                     /locus_tag="IV136_000603"
     CDS             33709..35148
                     /locus_tag="IV136_000603"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8464615.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35180..36223
                     /locus_tag="IV136_000604"
     CDS             35180..36223
                     /locus_tag="IV136_000604"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8464616.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36220..36798
                     /gene="purN"
                     /locus_tag="IV136_000605"
     CDS             36220..36798
                     /gene="purN"
                     /locus_tag="IV136_000605"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8464617.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36795..38330
                     /gene="purH"
                     /locus_tag="IV136_000606"
     CDS             36795..38330
                     /gene="purH"
                     /locus_tag="IV136_000606"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8464618.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38346..39620
                     /gene="purD"
                     /locus_tag="IV136_000607"
     CDS             38346..39620
                     /gene="purD"
                     /locus_tag="IV136_000607"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8464619.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39742..40656
                     /locus_tag="IV136_000608"
     CDS             39742..40656
                     /locus_tag="IV136_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8464620.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40789..42354)
                     /locus_tag="IV136_000609"
     CDS             complement(40789..42354)
                     /locus_tag="IV136_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8464621.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42347..43798)
                     /locus_tag="IV136_000610"
     CDS             complement(42347..43798)
                     /locus_tag="IV136_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8464622.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43812..44828)
                     /locus_tag="IV136_000611"
     CDS             complement(43812..44828)
                     /locus_tag="IV136_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8464623.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45048..46136
                     /locus_tag="IV136_000612"
     CDS             45048..46136
                     /locus_tag="IV136_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8464624.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46190..47716
                     /locus_tag="IV136_000613"
     CDS             46190..47716
                     /locus_tag="IV136_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8464625.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47718..48923
                     /locus_tag="IV136_000614"
     CDS             47718..48923
                     /locus_tag="IV136_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8464626.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49180..50157
                     /gene="guaC"
                     /locus_tag="IV136_000615"
     CDS             49180..50157
                     /gene="guaC"
                     /locus_tag="IV136_000615"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8464627.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50421..51845
                     /locus_tag="IV136_000616"
     CDS             50421..51845
                     /locus_tag="IV136_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8464628.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT        16528 a         8839 c        11382 g        15097 t
ORIGIN      
        1 catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat ataggaaatg
       61 tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc tgattattta
      121 ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa gggcaatgat
      181 ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac tgtcactttt
      241 ttatccaggt acttggatag ctactttagt agtcttggca ttattactat gtttaattgt
      301 tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta ttttgatgat
      361 aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg cttccggaat
      421 gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg cagttaaaga
      481 gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat taaccgtgct
      541 tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa atccctcgct
      601 ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca cttgcaagtt
      661 tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta catgattgta
      721 cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa cgttcaaaca
      781 aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg ggactcgaac
      841 ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt ccaacacatc
      901 tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag ctcaaaaatg
      961 ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg aatgatctta
     1021 ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct aggaatcatt
     1081 tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga caattaagtt
     1141 ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt ggcaaaattt
     1201 tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa gttctaactt
     1261 tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca aattagaagg
     1321 tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc aagaagaggg
     1381 cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg aagcaccaaa
     1441 cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact cagattccaa
     1501 ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac attttccgaa
     1561 tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca cgatgtgtat
     1621 gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact atccgttgtg
     1681 gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg atgaagaggc
     1741 agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga caaaagctat
     1801 tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat tattagaaga
     1861 aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa tctatgatcg
     1921 cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg atttgttcgt
     1981 tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag taggctggat
     2041 ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga atatgttgtc
     2101 ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg cgctaggcgg
     2161 ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac aaagagaata
     2221 tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa aagctgcttt
     2281 ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg aaaaattcgt
     2341 tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat ttaattggaa
     2401 ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga aacaaacagc
     2461 taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa aaccaaattt
     2521 ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga gttttcttaa
     2581 ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac ttcctaaagt
     2641 aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg aaacgttaat
     2701 cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga cgcaaaaaat
     2761 gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata atgaggaact
     2821 gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga ttgatcatgt
     2881 gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac tgttggatag
     2941 ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt ctgatatttc
     3001 tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg gtgtttgcga
     3061 attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag cattaagcta
     3121 ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga ttagttgtat
     3181 cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt tgagtcgaag
     3241 catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt cgcaaatttt
     3301 ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt tagaacaggt
     3361 gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca atgatttatt
     3421 tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag cattctatga
     3481 aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt agaccattct
     3541 tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag agaatagaat
     3601 gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac atgcaggcta
     3661 tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg agggagaagt
     3721 caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata cacatattca
     3781 cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag tgatgtctga
     3841 aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt caagcaaaga
     3901 acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac caggagctaa
     3961 aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag gggcacaaaa
     4021 cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag aagcttcagg
     4081 tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag agtttgtcaa
     4141 acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta cactagaaga
     4201 agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca atggtatgcg
     4261 tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag aacatgtctt
     4321 ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa tcctaatgga
     4381 aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg gtgggatgcc
     4441 tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa cagcacgtct
     4501 agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta aaaatgttgt
     4561 agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga ttccagcaat
     4621 cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg cagactttat
     4681 cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg aacgtttcca
     4741 cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt tttaaggttt
     4801 tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt aatttcctat
     4861 tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat tagcaaaata
     4921 aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg aacttatttg
     4981 acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact tcaacagttt
     5041 ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa tctgttggaa
     5101 aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt cgagctttca
     5161 tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt tggggattag
     5221 ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat aaaggggata
     5281 agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc ggaacgccta
     5341 tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc aatattggct
     5401 accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag atggaagcta
     5461 aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc ggccttttca
     5521 tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa ggagagataa
     5581 gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag ccgatggatt
     5641 atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca ttaggtgaag
     5701 taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta gtcaaagtgg
     5761 tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg aatattctac
     5821 cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca gctgcaacag
     5881 tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc aatgcatcct
     5941 tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta ttacaaatga
     6001 ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga atcgcatgtt
     6061 acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac cattatgatc
     6121 tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag gaccatgatg
     6181 ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa atgcaggaat
     6241 atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat ctacaagcgg
     6301 cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat gcgatagcag
     6361 agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg acgatccgaa
     6421 catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc agaaattgca
     6481 cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg tttaagggac
     6541 taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa aaagtattga
     6601 cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt catgtacctc
     6661 atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa atgaaaaaag
     6721 gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct attttaagag
     6781 ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat gaaacagagg
     6841 tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc cagcaattag
     6901 tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca gatgaggcat
     6961 tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa actggaaaaa
     7021 cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac atggatattc
     7081 gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta tacttggact
     7141 attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc cgcccattct
     7201 ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt ctttcgttca
     7261 atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt gacagtgagc
     7321 ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata aatgcactta
     7381 ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat ggtatttcgt
     7441 gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct ttttcagtcg
     7501 tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa cgttgcaaag
     7561 tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt atggtttcga
     7621 tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt ctaagccaag
     7681 ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc agatctgcgt
     7741 ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg attacgttca
     7801 tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga tcatcttcga
     7861 ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag atagacgatt
     7921 gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt gacgattgaa
     7981 tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc tagcgtaaat
     8041 attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt cacgatgaat
     8101 acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct ttcatgtttt
     8161 tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa aagtgaatgg
     8221 atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag catttcaaca
     8281 gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc aggtactttg
     8341 atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg ctggataaaa
     8401 tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg aagtttattc
     8461 atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc cccaagctcc
     8521 tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc taaaaaacgg
     8581 tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa atacatggat
     8641 tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc atatagatta
     8701 tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag aaaatgtttt
     8761 ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag attccttcat
     8821 ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct tgtggttcaa
     8881 gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg atgcctacgc
     8941 taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact tgattgtcaa
     9001 tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca taataaatag
     9061 ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa aattcttgtc
     9121 ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg taacttgggt
     9181 ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct aaattcgatg
     9241 caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca gtctgcagct
     9301 ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt ttatctgaca
     9361 cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa acaatcaaag
     9421 aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt aatggttttt
     9481 tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat tttaacatga
     9541 aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa aacaccactg
     9601 agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg tagaaatgga
     9661 aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt atacattgct
     9721 gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg attttgatgg
     9781 cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat ttggttttct
     9841 aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg gaggcatatc
     9901 gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac atgccgaatc
     9961 aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga tcacaccgat
    10021 ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca ttagtcgtcc
    10081 agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag tattgatcat
    10141 tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa aagcttaaag
    10201 gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg cagctttgat
    10261 tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac cggatgaagc
    10321 gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg aaagcaataa
    10381 caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc aacgttcgaa
    10441 tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt atacggaaca
    10501 aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat ctgtagaaga
    10561 gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg aaaatgaagc
    10621 aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg tagaagaaat
    10681 ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg tagaccttgc
    10741 aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca aaaacggtta
    10801 tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag atatagcaga
    10861 agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag aatcacaagc
    10921 agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag aactgaaatt
    10981 agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag cctacaattt
    11041 agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta aaatcgtaga
    11101 acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa agcaatatga
    11161 ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag aagcacaagc
    11221 tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg aagcactggc
    11281 agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag cagcactagc
    11341 aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca aggaatatgg
    11401 cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc gcgaagcagc
    11461 acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca gttcaggaga
    11521 aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt ctactcaaga
    11581 aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt tgtcgacacg
    11641 tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac aaaactcgat
    11701 gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa gactttttaa
    11761 agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg ctatttttct
    11821 tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat cgttctttct
    11881 tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca ggaggacgaa
    11941 gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt acacgttgga
    12001 tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac aacgtagcta
    12061 ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc acaataataa
    12121 ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag cttttgacaa
    12181 aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta gactttccaa
    12241 aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat gcagcaatca
    12301 tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca gctaaggcac
    12361 ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt ctcctttttg
    12421 gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt attcaagtat
    12481 aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc aaaacagtga
    12541 tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat tggccagctg
    12601 cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg cgtacgggca
    12661 tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt ttttttgctg
    12721 ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg cttgcctctc
    12781 gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat tatttagtgg
    12841 aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg attggattag
    12901 tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg aaggaaaacc
    12961 aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa tttcttttat
    13021 cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc attcgcgaaa
    13081 gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg ctatactacg
    13141 aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg ctggaaaatc
    13201 tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta ttgatttata
    13261 ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt attgaagaag
    13321 tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc gaggatcgag
    13381 cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag gcagaattct
    13441 cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg tcaaaaagct
    13501 gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaca
    13561 atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc ggagctgaac
    13621 ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc ggtaataagt
    13681 ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca
    13741 gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt gcatttgatc
    13801 caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt caccttcaat
    13861 catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc cgctttcgat
    13921 gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg ggtaatcagc
    13981 aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt ttgcctgaaa
    14041 agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga cggattgttc
    14101 gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt gagagctcaa
    14161 aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc ctccagctaa
    14221 ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga aaggtgcttg
    14281 gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact gctgataaag
    14341 tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt ctgctaagcg
    14401 catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata gaacgtgttt
    14461 gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta cagcaatgta
    14521 cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag cttttggaat
    14581 attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa ctgctgttag
    14641 ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac caagaccaac
    14701 gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat tgagtttctt
    14761 gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact tgaaacatta
    14821 aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag tagaatagtt
    14881 aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt gtattattag
    14941 aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt ccagaacaat
    15001 ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag aaagtaaaaa
    15061 atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa acgattattt
    15121 ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca cgcatcgcgc
    15181 aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa aataatcagc
    15241 aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg ggctatgatg
    15301 atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga gaaaaaatcg
    15361 aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag gcgaatgggg
    15421 aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt tggagcagtt
    15481 gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc gcatgttcaa
    15541 gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa agaaaagaac
    15601 agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac gtccaaacaa
    15661 ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga tcacaaatta
    15721 gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata cttgatcgac
    15781 aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc tggatttcca
    15841 ttagtgatgg tctcagctta ccggtctgtt atcatcccaa caacaagttt tcgaacaaaa
    15901 tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa agattactaa
    15961 agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag tcgatgtcgt
    16021 cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt acggagatca
    16081 gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg ttcgttatcc
    16141 aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac gttatgtcgg
    16201 taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat atctgaatga
    16261 attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc aaaagcgcaa
    16321 gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag cctttgtttt
    16381 ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg aagcgcaatc
    16441 ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag atggttatgg
    16501 agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg gaaaaagtac
    16561 cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc agaagaaagt
    16621 aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag gagacttcaa
    16681 agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg tccaaggagt
    16741 agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc aggaagccgc
    16801 acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa aaatcatcca
    16861 ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga gaagtggctg
    16921 atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat cgtaagagta
    16981 ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat ccgttcgatc
    17041 atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt aattgctgtg
    17101 tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc tgacattcca
    17161 gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta catgagcatg
    17221 ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc tttagagaca
    17281 gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac gtcgaaatat
    17341 acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag tgcaggtaaa
    17401 gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc tatcattgcg
    17461 acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc aaatgccatc
    17521 acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa atatcgggaa
    17581 actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag agcagatagt
    17641 ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa aaaagagaaa
    17701 tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt gttattatta
    17761 gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat gaaaaacgaa
    17821 taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg gcttgaaaag
    17881 tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga cgtgtatatt
    17941 gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa gagcgcacac
    18001 gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga tatccttttt
    18061 tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga tgaaagaaga
    18121 atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc agaacgataa
    18181 ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg ttctgtataa
    18241 aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga agaaaagaat
    18301 gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg ggcaagaaga
    18361 tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac acgatgcaac
    18421 agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg attatgaatt
    18481 ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag atgatatctg
    18541 ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg aaccattaga
    18601 tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct atttagcaac
    18661 gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt ttgcgattgc
    18721 ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa attggacatg
    18781 ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct atgctatttc
    18841 aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg agaaaggatt
    18901 gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc cagactatct
    18961 aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc cagaaccagg
    19021 tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata gagaagcggc
    19081 aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc ctgaagatat
    19141 ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg gtatcccttt
    19201 acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag aagctgctaa
    19261 gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag gagaacgtgg
    19321 tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg caactttgca
    19381 agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag tgaatgtctt
    19441 tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg aaaaagtcat
    19501 ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg cgtcaaacat
    19561 tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt tcttggatca
    19621 agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc tattgataac
    19681 agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt tgggaaatcc
    19741 agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc gttttttgaa
    19801 ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac tgatctttgg
    19861 tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct ttcgttcttt
    19921 gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt ggcgggagtt
    19981 gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac ctgcgctttc
    20041 ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg cttggcaatt
    20101 cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct cgtattatga
    20161 agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca cgttacctct
    20221 tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt ttatgacttt
    20281 tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact tttatgctct
    20341 ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg ctatttcgtg
    20401 gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat caaaaaaatg
    20461 ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa tggtttcaat
    20521 acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg atgatcgcag
    20581 gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt ccagagactt
    20641 ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca ttttttactt
    20701 tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt ctgagctcga
    20761 cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc tggttcgtcg
    20821 tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag tttattattt
    20881 acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat ttttttggac
    20941 aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa gagttagatg
    21001 aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt atttttcctt
    21061 tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa tgggacgact
    21121 tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg attgccatca
    21181 aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg atgtctacgt
    21241 tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt gtccagggga
    21301 tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga tgtttcttta
    21361 caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta atgctctttt
    21421 cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc gtgttgtcta
    21481 tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt acttggcaga
    21541 aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca ttacgttgtt
    21601 tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa agattggagc
    21661 ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata gtttttttag
    21721 tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt tgatcaaaag
    21781 gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat gccttttgta
    21841 tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt atccagggct
    21901 gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat gaagaaaaaa
    21961 tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat tgcctctacg
    22021 tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag gatggattat
    22081 gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg tatgccatca
    22141 caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac gtcagtagat
    22201 cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt tatttgcgaa
    22261 aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca aaaacagacg
    22321 ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac gaagataaag
    22381 gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga atggctgtta
    22441 aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca caatagcggc
    22501 gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg gttgaacagc
    22561 cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca taatgcggag
    22621 aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc taaagctgat
    22681 ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt agaagcagaa
    22741 aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc tattgaagat
    22801 acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc gctaaacgcg
    22861 tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg gatcgaaatg
    22921 aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga aggagcagcc
    22981 gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt aatcgtcgaa
    23041 caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct atttggtgaa
    23101 gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat tttgtcgatt
    23161 ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt gattgacgag
    23221 cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta gtttataaaa
    23281 tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa aaggggctat
    23341 gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata ggagaaaata
    23401 aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat tagattttac
    23461 aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg ttttcaatat
    23521 ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa acagttgcaa
    23581 gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt cataatatgg
    23641 atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt ggctatttca
    23701 cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca tagtaggttt
    23761 ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata aagtgaaaga
    23821 attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat taaaagtcga
    23881 cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa aacggtttgc
    23941 agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt ctgggatcgc
    24001 accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta gaaaagcaaa
    24061 aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta cgaaacaagt
    24121 gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag tattgattat
    24181 cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat gccagcaagc
    24241 tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag atggacgtca
    24301 gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt ccttgacgaa
    24361 tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac acaaaatgga
    24421 aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc agtagcggtg
    24481 cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta tttgatttca
    24541 attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa ccgatttttc
    24601 ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc actgatcttg
    24661 atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc tgggatcttt
    24721 gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc tatagtaaca
    24781 ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga gaaaatgggt
    24841 ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact tgcatttgtg
    24901 acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg ttcaatcgcg
    24961 gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat gattgatctg
    25021 agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt tggtaaacca
    25081 actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag catggtagaa
    25141 tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg agaagaagaa
    25201 ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat tttcaataca
    25261 ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg aatcaaaaca
    25321 agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt ccctaaaatc
    25381 ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt agtcatgttc
    25441 gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag caatgataaa
    25501 aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt tacgccgaca
    25561 ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat cgtaatgagt
    25621 agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga tgaagaaata
    25681 ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc agcgttttga
    25741 cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt ttgtttgagc
    25801 taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag cctgccgttc
    25861 atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt gaatattgtt
    25921 ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga atgtgaccta
    25981 acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa tgtttttctt
    26041 tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata tgaatgtaga
    26101 aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga gtacttcaga
    26161 ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt cttatgaaaa
    26221 aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac aaaacgctcg
    26281 aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc tgccgggtat
    26341 ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac gaacattaaa
    26401 tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg tagctactac
    26461 agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga tgctttccat
    26521 gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg ctaaaactgt
    26581 tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg atcgggatcg
    26641 tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg ggctttaaag
    26701 taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat tggcatattg
    26761 aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca gatgtcatta
    26821 cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc atgtcattta
    26881 ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa aaatcttttt
    26941 tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg acggatatcc
    27001 aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga ggcggctatg
    27061 atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc atggaccttt
    27121 taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag atttcgatca
    27181 tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa aacatccacc
    27241 acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat gtgatcgagg
    27301 aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt gttttagggg
    27361 tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct cctcgtcctc
    27421 acaattctgg acattattct attgaagctt gttctatgag tcagttcgat gcccatattc
    27481 gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat gcagtaatgg
    27541 tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat aagccagact
    27601 ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg ggacatatca
    27661 ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg aatatttggg
    27721 attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg aatatctgga
    27781 cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat gaagcttggg
    27841 cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat gcatcatttg
    27901 atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt gcgtttacac
    27961 gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt ctaacaagta
    28021 cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat attttacggg
    28081 aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac aaatatacag
    28141 tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga ttgaaacttg
    28201 ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg gcaaaaggag
    28261 tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg ccatttattg
    28321 aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatttctact caagttctcc
    28381 ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca agtattgaac
    28441 gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta gaagaatttt
    28501 ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg atcggttcag
    28561 aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc tatgaaaatg
    28621 tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc atcttgcctg
    28681 ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt aaaaatctaa
    28741 cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg atttatagcc
    28801 agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct tatgatcttg
    28861 ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg ctgttagaag
    28921 aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc tttgattatc
    28981 atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa taaagaagag
    29041 tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga agcagatccg
    29101 ttagcggttc tgcttctttt agtttattat attttcttat caataatagg aacctgattt
    29161 taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg aattaagcgt
    29221 aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc aagttttcga
    29281 gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa tgatttttga
    29341 aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct gtctgataaa
    29401 gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt tttcggaatg
    29461 tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc aaatcattat
    29521 atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct tgttttattg
    29581 catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt taattctaat
    29641 ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt cgtttctgat
    29701 aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt tgatttataa
    29761 aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta
    29821 atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc
    29881 caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt tagatcaagc
    29941 aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat tgaataatca
    30001 aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc attttattga
    30061 acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc tggaagtggt
    30121 tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga
    30181 attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg atccatttat
    30241 caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca gtgagctgaa
    30301 agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg tcggtttggt
    30361 tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc ttgcggatga
    30421 agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata tggacaaaga
    30481 cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat ataaccgtct
    30541 atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc
    30601 tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg
    30661 ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca acttgctgat
    30721 gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct tgcaaatgta
    30781 aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc
    30841 ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag ttcgcgagat
    30901 catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag gatttgatgg
    30961 ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg cgattgctcg
    31021 gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac cagtattcgg
    31081 tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg tactgagacg
    31141 aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc
    31201 ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc atggggaagg
    31261 aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc agattgcctt
    31321 tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta cgaacaaagc
    31381 agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact tgctaggatc
    31441 agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata
    31501 gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa tctatgccga
    31561 atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag gaagaatgcc
    31621 taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt cctataaaaa
    31681 ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc aaggccctgg
    31741 agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca aagcagaaag
    31801 tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag ttggcggaat
    31861 catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt cattgaggtt
    31921 cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg caggtatcgg
    31981 cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat ttgatccttg
    32041 ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat
    32101 ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg
    32161 tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag aagaagaaca
    32221 gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg
    32281 tttggaatta atcttgcatc attcggattc tctaataggt atacaggata tgggagcagc
    32341 aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac ttatattgac
    32401 attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga tgttatcgga
    32461 atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa tccaagaact
    32521 atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg acgggcaata
    32581 tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat tagctgaaga
    32641 tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat ttcagcagtt
    32701 agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc tgctgcagca
    32761 accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag tacaaaccaa
    32821 tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga cgaataaagc
    32881 gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg
    32941 acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta agcccttggc
    33001 gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt gggaattatc
    33061 gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg
    33121 aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac caatgattgg
    33181 tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca aaaaatcagg
    33241 ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg aattacaaaa
    33301 gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag
    33361 aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg ctcatgactg
    33421 ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc agctagggat
    33481 atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat caagatttgt
    33541 gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag atgctgctgt
    33601 tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg acggtcagat
    33661 caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa
    33721 gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc agacgccgct
    33781 agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg tgcaggtatc
    33841 gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct ttctgaggta
    33901 ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg acatgtacgt
    33961 tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt caaatttcat
    34021 gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc ccttagaaaa
    34081 gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat attgatgcat
    34141 ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc tttgaatgaa
    34201 gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc cgctttggac
    34261 ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata cgtgattgcc
    34321 tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt ccagcctggt
    34381 gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa ggacacgcag
    34441 ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa catcgcaggg
    34501 atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga atcgccagta
    34561 aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag cggttatgcg
    34621 gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca
    34681 tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt atctgctgta
    34741 cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt tcggggtacg
    34801 acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg
    34861 attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca aacgcgtaaa
    34921 gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg tgcagattct
    34981 ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa agatgctccg
    35041 tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt atatgactat
    35101 gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag
    35161 aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc
    35221 tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat
    35281 gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc aattaaaaga
    35341 gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc
    35401 agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc
    35461 acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa atgatccgtt
    35521 acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa atatggcgct
    35581 gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt atgatctagc
    35641 cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc
    35701 aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt actctcttgt
    35761 tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc cagaattgaa
    35821 aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc
    35881 gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag ggtttgtaga
    35941 aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg gctcatggcc
    36001 gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga tggagatgta
    36061 tgaaatcttc aatatgggca tcggtatggt cctggcagtc gaagaatcga aagtaaaaga
    36121 aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga aagtgatcgc
    36181 caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc tgtttttgct
    36241 tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa aggattggaa
    36301 tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa acgtgcaact
    36361 gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa aaaagagtat
    36421 gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt attagctgga
    36481 tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat catcaatatc
    36541 catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc ctttgaagct
    36601 ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga tactggacca
    36661 atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc tttagaagaa
    36721 aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat tattgaaaat
    36781 ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact gggattgtag
    36841 cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga ggaacaaagc
    36901 atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca gggtttcctg
    36961 aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt cttttaggaa
    37021 gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa ccaatcgact
    37081 ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat gtcgaaatag
    37141 cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa
    37201 accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta ctttcagaat
    37261 tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca aaagtctttc
    37321 gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg gtggaagaaa
    37381 aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa
    37441 atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag
    37501 cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat gcagatgctg
    37561 cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa cacatgaatc
    37621 cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg tatgaagcag
    37681 atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat ctgccaacag
    37741 cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat acacaagaag
    37801 ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac tttgcgcagg
    37861 aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt gtacaagatc
    37921 aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc
    37981 cagcagaagc aaaagcatta gtgtttgctt ggagagctgt caaacatgtg aaatctaatg
    38041 cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag atgaatcgag
    38101 tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt gcagttttag
    38161 ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa catggtataa
    38221 aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat atggcgaata
    38281 aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag agaggagtcg
    38341 gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata agtaaaaaac
    38401 ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg gggatgacaa
    38461 aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta atccattttg
    38521 ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg ttaaatggga
    38581 tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa gcagcagctc
    38641 tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa atcccgacag
    38701 ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg
    38761 cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc gtggctacca
    38821 cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga tttggggata
    38881 gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc cttttggcgt
    38941 ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag cgggcatttg
    39001 ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta ccgcaaattt
    39061 caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct ttgggaatga
    39121 aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct acgaaggaag
    39181 gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt
    39241 ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat cgtacagtag
    39301 agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct gccggttacc
    39361 ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag ctttctgttt
    39421 actatgctgg agtttcctca aataacggca aactagttgg agcaggagga cgtctttatt
    39481 tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac accgcgttaa
    39541 tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa gctgccaata
    39601 gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa caaacataat
    39661 cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt ttgttgtgta
    39721 tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga tcagaaagca
    39781 gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt
    39841 gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta tggaaacggt
    39901 gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg agaagatctt
    39961 tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat
    40021 atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt ggatgcttta
    40081 cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc gtttgatgaa
    40141 ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc
    40201 gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca atttggcttg
    40261 aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga agcaagtatc
    40321 gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat ccaagcatgg
    40381 tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa
    40441 ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac tggcttggct
    40501 tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac gatgaacgta
    40561 gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga agattggtac
    40621 gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc aggtcaaaag
    40681 ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga gatattctgt
    40741 ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt agttattttt
    40801 gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt ttttcttaaa
    40861 taaataactg aaatagttag ggtcattata accaatttca aaagcaatat ctgaaatacg
    40921 atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg tcagataatc
    40981 gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg gacttaatgc
    41041 aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt ctttgataaa
    41101 atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg agggcagagc
    41161 agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt cggcgctgat
    41221 tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt tcttttcttt
    41281 caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc gtgttcgagt
    41341 tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt ttttcgataa
    41401 tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc
    41461 ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg tttcaagtat
    41521 catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga caggaccaat
    41581 ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg tttgggctac
    41641 ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa atttgatgaa
    41701 tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa acttcaaata
    41761 atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg ctaataatac
    41821 ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca atgtttcact
    41881 gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca attcgaaaac
    41941 aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta ctgctttttc
    42001 tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat ctgcctgtac
    42061 ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata tgagacgagt
    42121 ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga aaggcatttg
    42181 gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg ccatctcacc
    42241 atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag ttatttgttc
    42301 ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat acatgtttgt
    42361 ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt ccttctcggt
    42421 atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg agacgcttgt
    42481 ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg gctaactctt
    42541 tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata actttgaatt
    42601 ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga cggacgtact
    42661 tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt aaaatagaaa
    42721 caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg tatcttattt
    42781 ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga atccagtctt
    42841 gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt
    42901 cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta taaataaaat
    42961 ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg tattgtttca
    43021 aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg ctagcggtaa
    43081 gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa
    43141 gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc tctaaaaagc
    43201 gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc actattgtta
    43261 atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg atctccacac
    43321 gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc tgattgatga
    43381 tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc tcgttttgat
    43441 aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt tctttaagtg
    43501 tatttgtatt atttttgctc tctcgaatat cattcctaag ttcttctagg atattttcct
    43561 cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt tccaaaactt
    43621 tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc gtctgttgat
    43681 aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata acagcaaata
    43741 gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt tttgtcatga
    43801 tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg ggaaaagaca
    43861 gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt tccaaaataa
    43921 cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac tacacaattg
    43981 acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt tacaatgatc
    44041 aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt
    44101 tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc taaatatcgt
    44161 ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg aacaatagtc
    44221 atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg attgattgat
    44281 tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc agcaagttct
    44341 aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc
    44401 cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc gggatcattc
    44461 gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc ttcttttaat
    44521 acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac tttataggca
    44581 atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt tcggtagaga
    44641 acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt tttccgccag
    44701 ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta
    44761 ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa aaataaaaca
    44821 cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag ataaaaggga
    44881 aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa aatccaagac
    44941 aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat gttaacgctt
    45001 tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat
    45061 ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta agtgcgtgtg
    45121 gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat
    45181 cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa aaactgggtt
    45241 ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca caaatagaaa
    45301 atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt tcggctttaa
    45361 ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat gatcgtttac
    45421 tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga gtaggtgtat
    45481 cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga ccattcacga
    45541 ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac aacggagtga
    45601 tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct ggacagacga
    45661 gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca aggatggata
    45721 atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg ccatatgatc
    45781 caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac
    45841 ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg atcattgccg
    45901 gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat acgattgaaa
    45961 tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa acttatgata
    46021 atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat aaagataatt
    46081 atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa aattaagagg
    46141 aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta tggcggattg
    46201 tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt tagattgcgt
    46261 gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa
    46321 atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg acggggaaat
    46381 cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat
    46441 cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag agaatatttt
    46501 tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag aaaagaaaac
    46561 atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag tatcccagat
    46621 tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat ccgtccgtct
    46681 gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag
    46741 attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac ataagctgaa
    46801 tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga cgattgagac
    46861 actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg gcagagattt
    46921 agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta aagtagaaga
    46981 ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca tcaacttgca
    47041 tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca gaactgaatt
    47101 cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta tattaaagga
    47161 aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac tcgcttatgt
    47221 ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg aaaacgtgac
    47281 gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa aagaagtaac
    47341 tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc atcaaattgt
    47401 cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc tgatgacaca
    47461 acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg caaaatatga
    47521 gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc
    47581 tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg aaggacgtat
    47641 tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa
    47701 agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac
    47761 taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg atcattttag
    47821 cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt ccattgaata
    47881 taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg atgttattag
    47941 ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa
    48001 ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc
    48061 ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat gcagggatac
    48121 cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca caagttgttc
    48181 taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct acgggttatt
    48241 taccagactt aagcgaagga agcagccttc acttcttgac attgatttta ggaatgatca
    48301 tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat
    48361 ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca gtcgttgtac
    48421 taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg atcattcttg
    48481 gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt cgacagattt
    48541 atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca aagcgaatca
    48601 cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta gttttagctg
    48661 ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat gcaatggcgg
    48721 cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg ggtgcgattg
    48781 tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc gtcggggtag
    48841 actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta gacttgtata
    48901 ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga ctgtgacaag
    48961 acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac
    49021 taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc ttgatttttt
    49081 tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa ttaataatgt
    49141 tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt tgattacgaa
    49201 gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg tgatacaacg
    49261 gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat gcagacgatt
    49321 attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat catgcatcgg
    49381 tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg attgattacc
    49441 tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt ggcagaagaa
    49501 tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa ctcagttatt
    49561 gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt
    49621 ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg
    49681 attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac tggaggctgg
    49741 caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt
    49801 gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg
    49861 attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga
    49921 atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga aaaacgaaat
    49981 gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac actagtggaa
    50041 atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct ggaagccatt
    50101 cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac aatctaaaaa
    50161 ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc
    50221 aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca gatacaagct
    50281 tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca gtcccttatg
    50341 ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag
    50401 acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga ccgatttatc
    50461 gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc
    50521 aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc accgttgtcc
    50581 atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc agcggatggt
    50641 ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg cggcaaatga
    50701 tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa gcaaagcaga
    50761 tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag cttttgccga
    50821 attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc
    50881 ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat
    50941 tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca atctttttga
    51001 ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct tctttttttt
    51061 accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg atggatgaag
    51121 aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg aaagcagtgc
    51181 ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa
    51241 agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat gtatttacgg
    51301 atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat ccggataatg
    51361 tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc ggttgggtga
    51421 taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg gagttttcga
    51481 tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt gatttgaaaa
    51541 tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct tggggagatt
    51601 cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg attacttggg
    51661 atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt cttggaattg
    51721 caccaagtct actttttggg caagatataa atggaatcag aatcaattac agtaggctgg
    51781 atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag tggttaacag
    51841 aataat
//
LOCUS       DACDEA010000010        51061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDEA010000010 DACDEA010000000
VERSION     DACDEA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.010"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..945
                     /locus_tag="IV136_000617"
     CDS             100..945
                     /locus_tag="IV136_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8464629.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1226..2446
                     /locus_tag="IV136_000618"
     CDS             1226..2446
                     /locus_tag="IV136_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8464630.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2443..5163
                     /locus_tag="IV136_000619"
     CDS             2443..5163
                     /locus_tag="IV136_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8464631.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5147..6091
                     /locus_tag="IV136_000620"
     CDS             5147..6091
                     /locus_tag="IV136_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8464632.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6160..7170)
                     /locus_tag="IV136_000621"
     CDS             complement(6160..7170)
                     /locus_tag="IV136_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8464633.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7350..7694)
                     /locus_tag="IV136_000622"
     CDS             complement(7350..7694)
                     /locus_tag="IV136_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8464634.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7687..8121)
                     /locus_tag="IV136_000623"
     CDS             complement(7687..8121)
                     /locus_tag="IV136_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8464635.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8259..8996
                     /locus_tag="IV136_000624"
     CDS             8259..8996
                     /locus_tag="IV136_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464636.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8989..10200
                     /locus_tag="IV136_000625"
     CDS             8989..10200
                     /locus_tag="IV136_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464637.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10407..11186
                     /locus_tag="IV136_000626"
     CDS             10407..11186
                     /locus_tag="IV136_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8464638.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11256..11918
                     /gene="trmB"
                     /locus_tag="IV136_000627"
     CDS             11256..11918
                     /gene="trmB"
                     /locus_tag="IV136_000627"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8464639.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12016..13770)
                     /gene="ade"
                     /locus_tag="IV136_000628"
     CDS             complement(12016..13770)
                     /gene="ade"
                     /locus_tag="IV136_000628"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8464640.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13902..14831
                     /gene="cpaF"
                     /locus_tag="IV136_000629"
     CDS             13902..14831
                     /gene="cpaF"
                     /locus_tag="IV136_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8464641.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14788..15837
                     /locus_tag="IV136_000630"
     CDS             14788..15837
                     /locus_tag="IV136_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8464642.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15834..16112
                     /locus_tag="IV136_000631"
     CDS             15834..16112
                     /locus_tag="IV136_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8464643.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16087..16557
                     /locus_tag="IV136_000632"
     CDS             16087..16557
                     /locus_tag="IV136_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8464644.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16574..16876
                     /locus_tag="IV136_000633"
     CDS             16574..16876
                     /locus_tag="IV136_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464645.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16893..17264
                     /locus_tag="IV136_000634"
     CDS             16893..17264
                     /locus_tag="IV136_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8464646.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17236..17595
                     /locus_tag="IV136_000635"
     CDS             17236..17595
                     /locus_tag="IV136_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464647.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17767..18774
                     /locus_tag="IV136_000636"
     CDS             17767..18774
                     /locus_tag="IV136_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8464648.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18799..19983
                     /locus_tag="IV136_000637"
     CDS             18799..19983
                     /locus_tag="IV136_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8464649.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20420..22003)
                     /locus_tag="IV136_000638"
     CDS             complement(20420..22003)
                     /locus_tag="IV136_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8464650.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22581..22724)
                     /locus_tag="IV136_000639"
     CDS             complement(22581..22724)
                     /locus_tag="IV136_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8464651.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22946..23425)
                     /locus_tag="IV136_000640"
     CDS             complement(22946..23425)
                     /locus_tag="IV136_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8464652.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23498..23782)
                     /locus_tag="IV136_000641"
     CDS             complement(23498..23782)
                     /locus_tag="IV136_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464653.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23860..24054)
                     /gene="ssrS"
                     /locus_tag="IV136_000642"
     ncRNA           complement(23860..24054)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV136_000642"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24126..25412)
                     /locus_tag="IV136_000643"
     CDS             complement(24126..25412)
                     /locus_tag="IV136_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8464654.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25550..26221
                     /locus_tag="IV136_000644"
     CDS             25550..26221
                     /locus_tag="IV136_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8464655.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26187..26675
                     /locus_tag="IV136_000645"
     CDS             26187..26675
                     /locus_tag="IV136_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8464656.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26688..27635
                     /gene="prmA"
                     /locus_tag="IV136_000646"
     CDS             26688..27635
                     /gene="prmA"
                     /locus_tag="IV136_000646"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8464657.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27636..28400
                     /locus_tag="IV136_000647"
     CDS             27636..28400
                     /locus_tag="IV136_000647"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8464658.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28633..30846
                     /locus_tag="IV136_000648"
     CDS             28633..30846
                     /locus_tag="IV136_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8464659.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30857..31303
                     /locus_tag="IV136_000649"
     CDS             30857..31303
                     /locus_tag="IV136_000649"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8464660.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31474..32733
                     /locus_tag="IV136_000650"
     CDS             31474..32733
                     /locus_tag="IV136_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8464661.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32822..33769)
                     /locus_tag="IV136_000651"
     CDS             complement(32822..33769)
                     /locus_tag="IV136_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8464662.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34163..35455
                     /locus_tag="IV136_000652"
     CDS             34163..35455
                     /locus_tag="IV136_000652"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8464663.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35470..37239
                     /gene="aspS"
                     /locus_tag="IV136_000653"
     CDS             35470..37239
                     /gene="aspS"
                     /locus_tag="IV136_000653"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8464664.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37348..38223
                     /locus_tag="IV136_000654"
     CDS             37348..38223
                     /locus_tag="IV136_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8464665.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38489..40372
                     /locus_tag="IV136_000655"
     CDS             38489..40372
                     /locus_tag="IV136_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8464666.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40447..40731)
                     /locus_tag="IV136_000656"
     CDS             complement(40447..40731)
                     /locus_tag="IV136_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8464667.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40721..41455)
                     /locus_tag="IV136_000657"
     CDS             complement(40721..41455)
                     /locus_tag="IV136_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8464668.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41584..42207
                     /locus_tag="IV136_000658"
     CDS             41584..42207
                     /locus_tag="IV136_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8464669.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42399..43520
                     /locus_tag="IV136_000659"
     CDS             42399..43520
                     /locus_tag="IV136_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8464670.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43527..46655
                     /locus_tag="IV136_000660"
     CDS             43527..46655
                     /locus_tag="IV136_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8464671.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46674..49868
                     /locus_tag="IV136_000661"
     CDS             46674..49868
                     /locus_tag="IV136_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8464672.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50014..50721)
                     /locus_tag="IV136_000662"
     CDS             complement(50014..50721)
                     /locus_tag="IV136_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8464673.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16707 a         8929 c        10613 g        14812 t
ORIGIN      
        1 gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact
       61 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
      121 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
      181 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
      241 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
      301 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
      361 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
      421 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
      481 gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc
      541 ccttataata atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt
      601 atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta
      661 aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct
      721 aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc
      781 gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca
      841 atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat
      901 aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt
      961 atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact
     1021 tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg
     1081 tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt
     1141 tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata
     1201 aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat
     1261 ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa
     1321 gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata acgttgattt
     1381 tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca
     1441 tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg
     1501 aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt
     1561 atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc
     1621 tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc
     1681 ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca
     1741 aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg
     1801 ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc
     1861 aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga
     1921 attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac
     1981 aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca
     2041 gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga
     2101 ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag actatttgtt
     2161 agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa
     2221 tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca
     2281 atactatcag acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga
     2341 agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa
     2401 gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc
     2461 gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag
     2521 ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata
     2581 ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca
     2641 gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc
     2701 tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat
     2761 gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact
     2821 ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg
     2881 ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat
     2941 cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta
     3001 aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt
     3061 ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa
     3121 ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta
     3181 tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt
     3241 cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa
     3301 ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt
     3361 tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa
     3421 ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag
     3481 attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa
     3541 gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt
     3601 tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac
     3661 ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca
     3721 tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca
     3781 atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac
     3841 aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa
     3901 gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa
     3961 aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat
     4021 cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata
     4081 gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa
     4141 gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa
     4201 gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag
     4261 atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg
     4321 aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta
     4381 tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg
     4441 aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa
     4501 gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa
     4561 gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga
     4621 gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa
     4681 ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca
     4741 gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt
     4801 ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac
     4861 ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg
     4921 gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt
     4981 gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct
     5041 aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat
     5101 taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg
     5161 tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag
     5221 aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat
     5281 cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt
     5341 cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca
     5401 tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc
     5461 tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc
     5521 gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt
     5581 tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac
     5641 aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt
     5701 gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc
     5761 gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat
     5821 aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg
     5881 tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat
     5941 aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt
     6001 gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa
     6061 ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa
     6121 aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt
     6181 ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga
     6241 agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac
     6301 attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac
     6361 tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc
     6421 tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta
     6481 ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt
     6541 caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt
     6601 aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga
     6661 gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa
     6721 tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc
     6781 atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata
     6841 ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc
     6901 taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc
     6961 atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga
     7021 ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc
     7081 gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt
     7141 agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta
     7201 tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc
     7261 aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat
     7321 agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca
     7381 gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta
     7441 aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt
     7501 tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc
     7561 agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg
     7621 ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa
     7681 aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc
     7741 ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct
     7801 gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt
     7861 gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat
     7921 cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc
     7981 tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa
     8041 agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt
     8101 gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat acttcagctt
     8161 aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg
     8221 tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg
     8281 tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg
     8341 gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca
     8401 ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga
     8461 aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg
     8521 aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg
     8581 atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg
     8641 actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct
     8701 ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag
     8761 ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga
     8821 tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata
     8881 acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg
     8941 attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt
     9001 tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg
     9061 aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct
     9121 aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt
     9181 tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt
     9241 tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct
     9301 tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg
     9361 gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg
     9421 aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt
     9481 cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat
     9541 tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt
     9601 ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat
     9661 aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga aattacggca
     9721 aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa
     9781 aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt
     9841 tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt
     9901 agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg
     9961 aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag
    10021 caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg
    10081 attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt
    10141 gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga
    10201 aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc
    10261 tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga
    10321 acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa
    10381 agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac
    10441 gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct
    10501 ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa
    10561 aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg
    10621 aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt
    10681 accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca
    10741 tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa
    10801 atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg
    10861 aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt
    10921 atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa
    10981 cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca
    11041 tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc
    11101 aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac
    11161 aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga
    11221 cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag
    11281 cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg
    11341 gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga
    11401 aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat attggagtag
    11461 aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta
    11521 acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag
    11581 tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc
    11641 gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa
    11701 tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat
    11761 atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata
    11821 ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag
    11881 ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa
    11941 aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc
    12001 tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa
    12061 ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga
    12121 taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg
    12181 tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat
    12241 gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat
    12301 cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc
    12361 aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag
    12421 ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac
    12481 cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac
    12541 tttttctacc aaatctttag tcactagact attcgggata atttcgatca catgggcttg
    12601 tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg
    12661 cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga
    12721 ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc
    12781 tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa
    12841 acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc
    12901 aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt
    12961 atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga
    13021 tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg
    13081 ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata
    13141 aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg
    13201 gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt
    13261 catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc
    13321 atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa
    13381 aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc
    13441 agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga
    13501 tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat
    13561 gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc
    13621 gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa
    13681 gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac
    13741 ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa
    13801 gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa
    13861 aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat
    13921 cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag
    13981 cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag
    14041 tctcttcggc agatggacag caactgatct caagatttaa gtatttggga cagatggata
    14101 tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag
    14161 ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc
    14221 tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta
    14281 ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca
    14341 agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag
    14401 atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac
    14461 agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg
    14521 gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc
    14581 gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac
    14641 tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt
    14701 tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat
    14761 cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag
    14821 aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa
    14881 ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg
    14941 tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt
    15001 gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa
    15061 cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa
    15121 ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg
    15181 ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg
    15241 gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta
    15301 ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct
    15361 cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat atattccttg
    15421 tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta
    15481 aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc
    15541 tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat
    15601 cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta
    15661 ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat
    15721 cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta
    15781 gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa
    15841 agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga
    15901 tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag
    15961 accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc
    16021 ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac
    16081 gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg
    16141 ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt tccagttctc
    16201 gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa
    16261 cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg
    16321 aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa
    16381 gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct
    16441 agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg
    16501 acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac
    16561 aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt
    16621 agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct
    16681 atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga
    16741 gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga
    16801 aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag
    16861 catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat
    16921 tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa
    16981 aagagtggct aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct
    17041 ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat
    17101 atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta
    17161 tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa
    17221 atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc
    17281 tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca
    17341 ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca
    17401 aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga
    17461 gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta
    17521 gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat
    17581 actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt
    17641 ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg
    17701 tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg
    17761 tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag
    17821 cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat
    17881 ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa
    17941 cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc
    18001 agactgtctc aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat
    18061 caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca
    18121 aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta
    18181 acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt
    18241 gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa
    18301 tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc
    18361 gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc
    18421 gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag
    18481 ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa
    18541 gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat
    18601 gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca
    18661 caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag
    18721 aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc
    18781 aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg
    18841 aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt
    18901 atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa
    18961 atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa
    19021 ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga
    19081 ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa
    19141 ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa
    19201 aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg
    19261 ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga
    19321 aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg
    19381 aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc
    19441 acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc attagctggg
    19501 gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa
    19561 aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta
    19621 ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat
    19681 attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg
    19741 acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga
    19801 catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg
    19861 ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta
    19921 ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc
    19981 taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg
    20041 tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt
    20101 acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt
    20161 ttatttattg attttcagga aaatcccaat attttatttt cattcatctt tataagggac
    20221 attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt
    20281 cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa
    20341 cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt
    20401 tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct
    20461 cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga
    20521 ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc
    20581 tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt
    20641 tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat
    20701 cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca
    20761 atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca
    20821 aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac
    20881 cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat
    20941 gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct
    21001 ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt
    21061 gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt
    21121 ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa
    21181 aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg
    21241 tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact
    21301 caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc
    21361 gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc
    21421 gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg
    21481 ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc
    21541 gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta
    21601 atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact
    21661 tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga
    21721 ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg
    21781 taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt
    21841 cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac
    21901 cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat
    21961 tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga
    22021 ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag
    22081 aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt
    22141 cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta
    22201 attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt
    22261 tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa
    22321 tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc
    22381 gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt
    22441 gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa
    22501 cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa
    22561 tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc
    22621 tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc
    22681 gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa
    22741 gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca
    22801 ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga
    22861 ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc
    22921 cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt
    22981 tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag
    23041 aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca
    23101 aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt
    23161 ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca
    23221 atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg
    23281 tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag
    23341 ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca
    23401 tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc
    23461 ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt
    23521 tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca
    23581 aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat
    23641 atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa
    23701 aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga
    23761 taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc
    23821 aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt
    23881 gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc
    23941 gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca
    24001 ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc
    24061 tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt
    24121 cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc
    24181 agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta
    24241 tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc
    24301 acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca
    24361 tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa
    24421 ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca
    24481 ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga
    24541 ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga
    24601 tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga
    24661 atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta
    24721 agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct
    24781 ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg
    24841 ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt
    24901 ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct
    24961 gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata
    25021 taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc
    25081 agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta
    25141 ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg
    25201 tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt
    25261 ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg
    25321 cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga
    25381 ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt
    25441 cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt
    25501 ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa
    25561 acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt
    25621 ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc
    25681 ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc
    25741 gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca
    25801 tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc
    25861 aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc
    25921 agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta
    25981 tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa
    26041 aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc
    26101 gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga
    26161 gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg
    26221 aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc
    26281 atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg
    26341 atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt
    26401 ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag
    26461 gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat
    26521 tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat
    26581 tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg
    26641 aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg
    26701 aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg
    26761 caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc
    26821 aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca
    26881 tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta
    26941 tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag
    27001 tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc
    27061 gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa
    27121 ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga
    27181 cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg
    27241 ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg
    27301 atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca
    27361 aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa
    27421 tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac
    27481 tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc
    27541 tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt
    27601 atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat
    27661 catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg
    27721 ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg
    27781 ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag
    27841 aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa
    27901 agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc
    27961 cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc
    28021 ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag
    28081 agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg
    28141 ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg
    28201 aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg
    28261 atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc
    28321 ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa
    28381 tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga
    28441 aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc
    28501 gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt
    28561 ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg
    28621 ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt
    28681 agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact
    28741 gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa
    28801 gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta
    28861 catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat
    28921 gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac
    28981 gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga
    29041 gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga
    29101 gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat
    29161 cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat
    29221 ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa
    29281 tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg gatttttgca
    29341 gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa
    29401 aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag
    29461 gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt
    29521 aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc
    29581 ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct
    29641 gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca
    29701 gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc
    29761 tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac
    29821 gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt
    29881 gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa
    29941 atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca
    30001 aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat
    30061 aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa
    30121 tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac
    30181 aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct
    30241 gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt
    30301 aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa
    30361 aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa
    30421 aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt
    30481 tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct
    30541 gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca
    30601 cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat
    30661 gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg
    30721 aataaagata aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta
    30781 aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat
    30841 ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt
    30901 cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga
    30961 aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt
    31021 tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag
    31081 tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga
    31141 agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag
    31201 tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa
    31261 tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac
    31321 tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct
    31381 tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt
    31441 atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc
    31501 aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc
    31561 catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc
    31621 tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct
    31681 atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga
    31741 gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa
    31801 gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa
    31861 tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt
    31921 atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct
    31981 atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa
    32041 aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga
    32101 aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta
    32161 cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact
    32221 gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta
    32281 aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct
    32341 ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata
    32401 ccagaaggca gttgggttat tttagattta tatggaacag accatgatga acgaacagtt
    32461 gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat
    32521 aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt
    32581 gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag
    32641 tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat
    32701 ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg
    32761 aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg
    32821 atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat
    32881 ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag
    32941 acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc
    33001 ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt
    33061 ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt
    33121 catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt
    33181 ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat
    33241 cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt
    33301 ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg
    33361 attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag
    33421 tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa
    33481 atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat
    33541 catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag
    33601 cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc
    33661 ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc
    33721 caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct
    33781 cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg
    33841 gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg
    33901 aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg
    33961 aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg
    34021 tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc
    34081 gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata
    34141 gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt
    34201 gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga
    34261 ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag
    34321 tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga
    34381 acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca
    34441 taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt
    34501 tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc
    34561 atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt
    34621 caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac
    34681 gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag
    34741 tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa
    34801 acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc
    34861 tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat
    34921 cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag
    34981 tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct
    35041 agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg
    35101 attactcttg actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc
    35161 ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat
    35221 tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt
    35281 taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc
    35341 tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga
    35401 agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga
    35461 aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa
    35521 caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt
    35581 atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa
    35641 gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa
    35701 gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg
    35761 attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat
    35821 gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca
    35881 aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta
    35941 gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc
    36001 gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa
    36061 tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc
    36121 gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta
    36181 gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt
    36241 gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc
    36301 tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa
    36361 ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt
    36421 gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga
    36481 aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg
    36541 ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca
    36601 gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat
    36661 aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta
    36721 ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa
    36781 ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa
    36841 gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc
    36901 gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa
    36961 gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc
    37021 ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac
    37081 cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa
    37141 aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg
    37201 ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga
    37261 tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag
    37321 ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat
    37381 gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg
    37441 aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt
    37501 ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat
    37561 ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt
    37621 ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc
    37681 ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt
    37741 gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt
    37801 cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt
    37861 tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc
    37921 gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc
    37981 gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc
    38041 acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg
    38101 atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa
    38161 gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt
    38221 tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc
    38281 agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc
    38341 ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag
    38401 tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa
    38461 aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg
    38521 ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca
    38581 ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc
    38641 tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat
    38701 ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt
    38761 gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt
    38821 tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta
    38881 tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg
    38941 tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag
    39001 aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat
    39061 cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt
    39121 ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa
    39181 catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact
    39241 gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct
    39301 cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt
    39361 gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta
    39421 tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca
    39481 tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg aactttctta
    39541 tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa
    39601 aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt
    39661 acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga
    39721 tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa
    39781 caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc
    39841 caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat
    39901 gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt
    39961 cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt
    40021 gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga
    40081 tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac
    40141 gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa
    40201 tatgacgctg attgattggt acagctacag taatgaccat agtgactggt tttatgatga
    40261 tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt
    40321 gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca
    40381 aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt
    40441 taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt
    40501 ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga
    40561 aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc
    40621 accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata
    40681 aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct
    40741 cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt
    40801 tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc
    40861 caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct
    40921 gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta
    40981 gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga
    41041 ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat
    41101 tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga
    41161 tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt
    41221 ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa
    41281 aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac
    41341 gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga
    41401 taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt
    41461 ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag
    41521 gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag
    41581 actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga
    41641 aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct
    41701 ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc
    41761 agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga
    41821 tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta
    41881 aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct
    41941 acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca
    42001 gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg
    42061 cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg
    42121 gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat
    42181 ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa
    42241 tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg
    42301 ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt
    42361 gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg
    42421 attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac
    42481 tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag
    42541 acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg
    42601 tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca
    42661 gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac
    42721 agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt
    42781 actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc
    42841 aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag
    42901 tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg
    42961 aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac
    43021 taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc
    43081 cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga
    43141 caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa
    43201 cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt
    43261 acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc
    43321 aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc
    43381 gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg
    43441 aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag
    43501 aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg
    43561 gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga
    43621 tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt
    43681 ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac
    43741 cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg
    43801 aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag
    43861 ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac
    43921 gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc
    43981 aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata
    44041 aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat
    44101 taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc
    44161 ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg
    44221 ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag
    44281 aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg
    44341 aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag
    44401 ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc
    44461 aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt
    44521 cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt
    44581 ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa
    44641 aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag
    44701 acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc
    44761 aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg
    44821 aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc
    44881 aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac
    44941 tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac
    45001 aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt
    45061 tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa
    45121 aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg
    45181 agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc
    45241 ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg
    45301 aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa
    45361 aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg
    45421 ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat
    45481 tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag
    45541 aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga
    45601 tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag
    45661 caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg
    45721 aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt
    45781 tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag
    45841 cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag
    45901 ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag
    45961 agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat
    46021 acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt
    46081 atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat
    46141 tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc
    46201 aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat
    46261 ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct
    46321 atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta
    46381 ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg
    46441 tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg
    46501 aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta
    46561 gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta
    46621 gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat
    46681 ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt
    46741 tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat
    46801 tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt
    46861 gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa
    46921 atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg
    46981 agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg
    47041 tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag
    47101 agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat
    47161 atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg
    47221 ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa
    47281 tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac
    47341 aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc
    47401 tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc
    47461 aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag
    47521 aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg
    47581 gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt
    47641 ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac
    47701 cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc
    47761 ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag
    47821 caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg
    47881 acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag
    47941 agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg
    48001 aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct
    48061 tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc
    48121 ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg
    48181 atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg
    48241 atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac
    48301 aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc
    48361 atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt
    48421 ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac
    48481 agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt
    48541 tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga
    48601 aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg
    48661 ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg
    48721 ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg
    48781 ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt
    48841 atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca
    48901 gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg
    48961 ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag
    49021 gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga
    49081 ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa
    49141 cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact
    49201 tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga
    49261 tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta
    49321 ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg
    49381 ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct
    49441 tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac
    49501 cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga
    49561 tttccttaaa ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt
    49621 atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg
    49681 gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca
    49741 tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag
    49801 aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg
    49861 aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg
    49921 attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc
    49981 ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc
    50041 taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg
    50101 ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt
    50161 gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc
    50221 cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt
    50281 gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt
    50341 cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt
    50401 tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa
    50461 gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg
    50521 atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc
    50581 actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac
    50641 gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc
    50701 aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca
    50761 aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc
    50821 ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa
    50881 aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat
    50941 tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc
    51001 tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt
    51061 t
//
LOCUS       DACDEA010000011        50266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDEA010000011 DACDEA010000000
VERSION     DACDEA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.011"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(177..1433)
                     /locus_tag="IV136_000663"
     CDS             complement(177..1433)
                     /locus_tag="IV136_000663"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8464674.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2013..3314
                     /locus_tag="IV136_000664"
     CDS             2013..3314
                     /locus_tag="IV136_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8464675.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3383..3982)
                     /locus_tag="IV136_000665"
     CDS             complement(3383..3982)
                     /locus_tag="IV136_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8464676.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4236..5117
                     /locus_tag="IV136_000666"
     CDS             4236..5117
                     /locus_tag="IV136_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464677.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5130..6740
                     /locus_tag="IV136_000667"
     CDS             5130..6740
                     /locus_tag="IV136_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8464678.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6817..7476)
                     /locus_tag="IV136_000668"
     CDS             complement(6817..7476)
                     /locus_tag="IV136_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8464679.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7759..8424)
                     /locus_tag="IV136_000669"
     CDS             complement(7759..8424)
                     /locus_tag="IV136_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464680.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8571..9176)
                     /gene="dhaL"
                     /locus_tag="IV136_000670"
     CDS             complement(8571..9176)
                     /gene="dhaL"
                     /locus_tag="IV136_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8464681.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9204..10187)
                     /gene="dhaK"
                     /locus_tag="IV136_000671"
     CDS             complement(9204..10187)
                     /gene="dhaK"
                     /locus_tag="IV136_000671"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8464682.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(10189..10584)
                     /gene="dhaM"
                     /locus_tag="IV136_000672"
     CDS             complement(10189..10584)
                     /gene="dhaM"
                     /locus_tag="IV136_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8464683.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            10738..11058
                     /locus_tag="IV136_000673"
     CDS             10738..11058
                     /locus_tag="IV136_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8464684.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(11170..12039)
                     /locus_tag="IV136_000674"
     CDS             complement(11170..12039)
                     /locus_tag="IV136_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8464685.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(12135..13439)
                     /locus_tag="IV136_000675"
     CDS             complement(12135..13439)
                     /locus_tag="IV136_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8464686.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(13436..13945)
                     /locus_tag="IV136_000676"
     CDS             complement(13436..13945)
                     /locus_tag="IV136_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464687.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(14120..16255)
                     /locus_tag="IV136_000677"
     CDS             complement(14120..16255)
                     /locus_tag="IV136_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8464688.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(16338..17993)
                     /locus_tag="IV136_000678"
     CDS             complement(16338..17993)
                     /locus_tag="IV136_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8464689.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(17995..18309)
                     /locus_tag="IV136_000679"
     CDS             complement(17995..18309)
                     /locus_tag="IV136_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8464690.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            18479..18739
                     /locus_tag="IV136_000680"
     CDS             18479..18739
                     /locus_tag="IV136_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8464691.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            18753..19067
                     /locus_tag="IV136_000681"
     CDS             18753..19067
                     /locus_tag="IV136_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8464692.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(19797..20351)
                     /locus_tag="IV136_000682"
     CDS             complement(19797..20351)
                     /locus_tag="IV136_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8464693.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(20477..22406)
                     /locus_tag="IV136_000683"
                     /pseudo
     CDS             complement(20477..22406)
                     /locus_tag="IV136_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(22712..23428)
                     /locus_tag="IV136_000684"
     CDS             complement(22712..23428)
                     /locus_tag="IV136_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8464694.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(23429..25252)
                     /gene="glpO"
                     /locus_tag="IV136_000685"
     CDS             complement(23429..25252)
                     /gene="glpO"
                     /locus_tag="IV136_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8464695.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(25266..26762)
                     /gene="glpK"
                     /locus_tag="IV136_000686"
     CDS             complement(25266..26762)
                     /gene="glpK"
                     /locus_tag="IV136_000686"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8464696.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(26898..28358)
                     /locus_tag="IV136_000687"
     CDS             complement(26898..28358)
                     /locus_tag="IV136_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8464697.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(28348..29643)
                     /locus_tag="IV136_000688"
     CDS             complement(28348..29643)
                     /locus_tag="IV136_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8464698.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(29975..30652)
                     /locus_tag="IV136_000689"
     CDS             complement(29975..30652)
                     /locus_tag="IV136_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8464699.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(30682..31773)
                     /locus_tag="IV136_000690"
     CDS             complement(30682..31773)
                     /locus_tag="IV136_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8464700.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(31874..32308)
                     /locus_tag="IV136_000691"
     CDS             complement(31874..32308)
                     /locus_tag="IV136_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8464701.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(32892..34349)
                     /gene="ascB"
                     /locus_tag="IV136_000692"
     CDS             complement(32892..34349)
                     /gene="ascB"
                     /locus_tag="IV136_000692"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8464702.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(34346..36241)
                     /locus_tag="IV136_000693"
     CDS             complement(34346..36241)
                     /locus_tag="IV136_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464703.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(36299..38074)
                     /locus_tag="IV136_000694"
     CDS             complement(36299..38074)
                     /locus_tag="IV136_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8464704.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(38124..39620)
                     /locus_tag="IV136_000695"
     CDS             complement(38124..39620)
                     /locus_tag="IV136_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8464705.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(39814..41436)
                     /locus_tag="IV136_000696"
     CDS             complement(39814..41436)
                     /locus_tag="IV136_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8464706.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(41764..43077)
                     /locus_tag="IV136_000697"
     CDS             complement(41764..43077)
                     /locus_tag="IV136_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8464707.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(43322..43546)
                     /locus_tag="IV136_000698"
     CDS             complement(43322..43546)
                     /locus_tag="IV136_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464708.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(43566..43934)
                     /locus_tag="IV136_000699"
     CDS             complement(43566..43934)
                     /locus_tag="IV136_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8464709.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(44130..44840)
                     /locus_tag="IV136_000700"
     CDS             complement(44130..44840)
                     /locus_tag="IV136_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464710.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(44858..45355)
                     /locus_tag="IV136_000701"
     CDS             complement(44858..45355)
                     /locus_tag="IV136_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8464711.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(45392..45844)
                     /locus_tag="IV136_000702"
     CDS             complement(45392..45844)
                     /locus_tag="IV136_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8464712.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(45979..46584)
                     /locus_tag="IV136_000703"
     CDS             complement(45979..46584)
                     /locus_tag="IV136_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8464713.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(47034..47654)
                     /locus_tag="IV136_000704"
     CDS             complement(47034..47654)
                     /locus_tag="IV136_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8464714.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(47656..48525)
                     /locus_tag="IV136_000705"
     CDS             complement(47656..48525)
                     /locus_tag="IV136_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8464715.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(48548..48955)
                     /locus_tag="IV136_000706"
     CDS             complement(48548..48955)
                     /locus_tag="IV136_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8464716.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(48974..50263)
                     /locus_tag="IV136_000707"
     CDS             complement(48974..50263)
                     /locus_tag="IV136_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8464717.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        14209 a        10657 c         8598 g        16802 t
ORIGIN      
        1 aattatccga attgttcaca cttttgaaat aattaaaaaa aacgagggaa tttacaaatg
       61 taaactccct cgcaaagatt gtattaacga caaccagcgt tctattttag gatgggtttc
      121 aaaattggaa tgttgtaata gtatttccct ttttcaaggt aaaaacttac taaattctaa
      181 tcaattactt tggccaaaaa gtaatttttc ttaccttttc gaataaccac aaacttacca
      241 tcgaattcat cggatggatt tatgacaaaa tttaaatcgg taacgcggtc accgttaata
      301 cttatagctc cgtttgaaac atcttcacgt gcttgacgtt tagatggttc aatttttgtg
      361 gaaacaagca gttccacgat attttcaggt gtgcttgaaa tttcaacgtt cggcatttta
      421 ccgaatcctt gcgcgatttc ttccgcattt agttctttaa tatttccaga gaacaatgct
      481 tgcgttattt tttgcgcttc tttcaagtct tcttcactat gcacaaatct tgtaacttct
      541 tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt tttcgctaag
      601 tcttcgattt cttcttgaga caagaaagta aagaatttca agtatttcac tacatcgcga
      661 tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg aagttttctt cggatcaagc
      721 caaacggcac cgcctgcagt ttttccgaat ttcgttccgt cagccttcag catcaatggg
      781 atcgttaaac cgaacgcttc tgcttctggt ccttctaatt ttcggattag gtctaatcca
      841 gctgtgatat ttccccattg gtctgctcca ccgatttgca attgcacatc gtatgtttta
      901 tgcagatgca aaaagtcgat agattgcagg atttggtaag taaactctgt aaatgagatt
      961 cctacttcca aacggctggc aacgatatct tttgctaaca tcgtgttgat gttgaagttt
     1021 ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc gtagttgttc
     1081 acaaatgtga tatttgcatc tttaccaaac aattttctca tttgattgga aagtgcgccc
     1141 acgttatgtt gcacttgttc catcgtttgc aacacacgtt ctgttttgcg gccgcttggg
     1201 tcgccgatcg ttcctgttcc gccgccaatc agaatatatg gatgatggcc tgccaattgg
     1261 aatcttttca tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc accagttgga
     1321 tcaacgccgc aataaagtga aatgctcttt tcttcaacga gctcgcgaag tctttcttcg
     1381 tttgtttgct gattaatggc gtcgcgccaa gttaattcat caataatatt cattttactg
     1441 acctccgtgt agttttttta tgaaagcagg aacctgctag catgtgtaat taattatttc
     1501 ttcaggatag gataggaaat aaaaaaagtc ccttggcata taagccaagg gacgaaatct
     1561 cgcggtacca cccaatttgt atgtctagat atccaacata cctctcgaac atttaacgct
     1621 gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat tcgctttaaa gcatgtactg
     1681 gtttgcagcg accaccagct ttctgaaaca ttggctttaa caactacttc gtttcgatct
     1741 tcgtcttgtt attattaata tgtgtctatc atagagcgca actttttctt tgtcaacaga
     1801 aaaaggatat tttttttaaa ttcctcattt tttctttttt gaatccgctc taattccgaa
     1861 cattttattt tcaattattg ttaaaagaaa taactttttc tgtatttttt ctgcaattta
     1921 tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg aatgggacaa
     1981 gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa attattttag
     2041 gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt gtcatccgat
     2101 catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac
     2161 cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa agctacgatt
     2221 atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact gaaacacaac
     2281 agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct gatcctactc
     2341 agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc ttccactctc
     2401 aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac cgttcgatcg
     2461 aacaacttta tcaaaatgat tttgttttag ttaatttgaa tgatttgatc caaaaagacg
     2521 aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa ccattggttc
     2581 tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt cctagccgat
     2641 tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat aaagaagaaa
     2701 tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat cctgattttt
     2761 cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt ttaggttatc
     2821 gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta
     2881 aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat acatggggtc
     2941 acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc
     3001 aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt ggtgcagaca
     3061 tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag
     3121 gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg
     3181 atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct gatttagacg
     3241 gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac aaagaagcac
     3301 gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga cattttatcg
     3361 tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg atcaggactt
     3421 ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta gaaattcctt
     3481 ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa tccacttttc
     3541 cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct tgcgtatacg
     3601 catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc atcaaatcga
     3661 tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc atggcaatca
     3721 gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat
     3781 tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa tatttaaaaa
     3841 tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact ccagcggttc
     3901 ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg
     3961 ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt ttataatata
     4021 tatagttcaa atttattctg aggtttatta taactatcta ttgagaaagt atcaagctag
     4081 caattccttg tttatgaatt cattgacaga aatttataaa aagaatatta tgtactaaaa
     4141 taataaaaaa agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt
     4201 tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac
     4261 atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt
     4321 caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga
     4381 cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat
     4441 gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt
     4501 ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga
     4561 gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa
     4621 aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt
     4681 cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt
     4741 tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata
     4801 ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg
     4861 atgaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa
     4921 cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag
     4981 gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt
     5041 ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga
     5101 gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc
     5161 ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca
     5221 ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag
     5281 ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag
     5341 cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt
     5401 gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag
     5461 aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt
     5521 tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg atcagttgta
     5581 cgatgatttt tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat
     5641 ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc
     5701 caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac
     5761 gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt
     5821 atttgaccta tccatttaca actaacaact ggattccttt gctttatttg tttttgttca
     5881 gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct
     5941 atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt
     6001 tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg
     6061 gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa
     6121 tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca
     6181 tggtcgtctt gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta
     6241 cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac
     6301 tttattggac caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt
     6361 ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat
     6421 tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta
     6481 gtggctttct attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt
     6541 actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa
     6601 cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc
     6661 ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa
     6721 gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg
     6781 ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt
     6841 atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct ctaataattt
     6901 aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt
     6961 cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc
     7021 gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa
     7081 tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc
     7141 cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa
     7201 agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac
     7261 tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc
     7321 cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata
     7381 gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa
     7441 aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct
     7501 atttctaata aagaaacata acttccctta ttccaactga taattttctt ttatcatagc
     7561 ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt
     7621 tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta aatatttact
     7681 ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca
     7741 ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc
     7801 tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa
     7861 gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt
     7921 ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg
     7981 cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag
     8041 caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat
     8101 caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg
     8161 acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca
     8221 gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag
     8281 ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt
     8341 atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg
     8401 ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc
     8461 tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa
     8521 gaacagccaa acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga
     8581 ttgatcacat ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata
     8641 tgtccgattg cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg
     8701 gtgtgttctt ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct
     8761 gtgactggca gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt
     8821 tgaatacccg ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa
     8881 tcgacctctt ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa
     8941 gccccaccga ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt
     9001 tcaggatgat cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt
     9061 ccatggtctc catcgccaat cggggtatct agatcactga gatattgttt gttctgattg
     9121 attgcctctg caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta
     9181 tcaccctttt atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa
     9241 tctttccatt gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt
     9301 gaggtcataa aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga
     9361 gctaaaacat cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg
     9421 actagtacac ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt
     9481 tcctttgcca gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg
     9541 ataccaacac catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct
     9601 ggtacagttg ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct
     9661 cccagttctt tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg
     9721 tgtacaagga cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg
     9781 gcgatatcat catctacgat cacactttct acttcaatat cgtccatagc agctaattcc
     9841 tttgccatat cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct
     9901 tttccactat cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac
     9961 acatcaccaa gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt
    10021 tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct
    10081 acaacacggg aagattctac acgatgaaca agttctggat agctttttac taatccttcc
    10141 agcatttctt cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc
    10201 tttctttccg gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc
    10261 agtgatggct gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg
    10321 ctgctgctct tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat
    10381 atccgcaaaa acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt
    10441 catcgggtct gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc
    10501 ggatttttgc atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga
    10561 aataagcaaa atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat
    10621 tggtttcgct ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa
    10681 tatgtgtagt gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg
    10741 attacaacaa caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt
    10801 tttggtgagg tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat
    10861 atctttggcg gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca
    10921 cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa
    10981 atggactatg aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca
    11041 gctgtctcca cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat
    11101 gtagtggcac tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc
    11161 ctctcttttt tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc
    11221 ccctgctaag gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact
    11281 tgttgatttg gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac
    11341 tgatttttct cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc
    11401 agcttttttt gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat
    11461 tgcttgactg gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg
    11521 agcaacctga tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc
    11581 tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg
    11641 gtcatttccc actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc
    11701 tttcaatttg ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa
    11761 gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga
    11821 agatgaaatc gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga
    11881 ggcaccgttt tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc
    11941 agaagtttcc gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca
    12001 gattaacggc ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat
    12061 ttcttattat aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa
    12121 aaacggtgca tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa
    12181 cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc
    12241 cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca
    12301 tctacaatca gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac
    12361 acaaaactga atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc
    12421 ccgattaatc cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc
    12481 aatgtttgat caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact
    12541 tttttgaaat tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga
    12601 tatagcatct tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca
    12661 gataaccagt catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct
    12721 tgtctgaatc cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg
    12781 tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt
    12841 gagaattcaa ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt
    12901 gcagtgaccg cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc
    12961 agtgcatctg gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca
    13021 aaggcaagtc cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca
    13081 cgaagattag cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa
    13141 ccatcgtctg tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg
    13201 attgtatatt cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt
    13261 tcgacactca agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa
    13321 caccagacac caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa
    13381 tacataatag acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt
    13441 tcagttccac cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct
    13501 cttcggagac agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg
    13561 tatacacttc ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca tattcaaaag
    13621 atttatttac ttttttgtat agatttcgat tgttccacct agtcaataca aatgaggtaa
    13681 tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc attttctttt
    13741 ctgataaaaa agcgtacaag gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa
    13801 agacccatat aaaaggaaga acaacgatca tccatcctaa aagagccact aacgtttgcc
    13861 aaatctttaa ccagtaatta ccttttgaaa aatacttatc tttcaccaaa attgatgcaa
    13921 cttcttctct tgttactttt ttcatatcct cacctcacgt ttttttgtta cgacgtagga
    13981 gtttaccata catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa
    14041 gaacaaaaca tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt
    14101 tttatttttt atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc
    14161 tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg
    14221 gatataagat tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc
    14281 agttttctaa tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt
    14341 ctgtttgggt gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta
    14401 ttttcaacaa ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc
    14461 ggatcgatac gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga
    14521 ttgctccgct agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt
    14581 cgtcgaaaca acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct
    14641 cgtcacctac taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg
    14701 ccagggtata atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct
    14761 tttcaacaag taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg
    14821 gttgccaagc ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct
    14881 catccaccca tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa
    14941 gtttccttgc ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata
    15001 attccaactg cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg
    15061 cttgtttcaa aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc
    15121 tatcgatcaa tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag
    15181 aaaggactgt ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca
    15241 aaggatctga ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct
    15301 ccgatacatt ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg
    15361 ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg
    15421 atggtaggac attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt
    15481 catcggatgt catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag
    15541 cagaggtttt accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc
    15601 gaatgattgt gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct
    15661 catcttggcc taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa
    15721 acttgaccaa acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga
    15781 ttttattttg acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg
    15841 caattggtga acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg
    15901 taccgatata aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa
    15961 agtagggact tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt
    16021 tgatattcat ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt
    16081 tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt
    16141 ttccttctat ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct
    16201 tttgatacac gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca
    16261 ttctcctctt tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc
    16321 aagcttttta acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga
    16381 tataaagaaa atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt
    16441 tccgctagat agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct
    16501 aattcattta atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg
    16561 cccttttcca attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct
    16621 gtcggcaatt catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca
    16681 tatcctagca tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct
    16741 aattccggta aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc
    16801 cgtttatcca caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga
    16861 tggaagatca acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt
    16921 cctgatacat gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa
    16981 ccggtggatg cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga
    17041 tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt
    17101 gaagcaagag aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc
    17161 cccaccgcat aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg
    17221 cgtatgccta attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg
    17281 gataaaataa ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg
    17341 tggtcttcaa atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat
    17401 tctttttcta taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt
    17461 tcgatcacca tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg
    17521 aatcctgcac caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta
    17581 tcgatatctg gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc
    17641 gatggaacaa ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt
    17701 ttttgaccct catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca
    17761 tgatttggac gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga
    17821 agattttccc gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa
    17881 gaaacatacg gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc
    17941 cgtgtagcag cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt
    18001 ccctcctact gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata
    18061 accagatttc tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac
    18121 atagacggtc ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt
    18181 agctcctttt atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg
    18241 attttccaaa agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag
    18301 caaattcatt ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac
    18361 aggagcttta ttaatatgtc aaccaaaatg actcttcatt tttttcacta ctttttatat
    18421 ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag aatgtgttat
    18481 ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg
    18541 tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc
    18601 tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg
    18661 ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa
    18721 gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat
    18781 tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa
    18841 cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg
    18901 cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta
    18961 atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg
    19021 atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg
    19081 cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga
    19141 aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag aacagccaaa
    19201 caaaaatagc ttctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    19261 gaacaccgtt taaggaagag gttgtgaaat cagcggtttg acctaagtat tagagaaaaa
    19321 aacgggaaaa tagctcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
    19381 ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt agtttcgagt attaagaaaa
    19441 attttgtgaa aatggatttc tacatttttg cataaatttg gcttaatacc gagaaactgg
    19501 cttttgaata ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag
    19561 aaacaaacgg aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca
    19621 gctgattgaa caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag
    19681 aaataatttc aaaaacaact tctcctgttt ttggttcggt tggcttatta tcgagaagta
    19741 gctactggga gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat
    19801 tttctttgaa acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg
    19861 taagtgatga cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc
    19921 aaaaggatca tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca
    19981 cttaatttga tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt
    20041 atcaagaata gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc
    20101 agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc
    20161 tgattatatc tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg
    20221 accgctcctg ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa
    20281 aaaatacgga atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg
    20341 cttaatttca ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca
    20401 cggggaataa aaccaatgat ttcactgttt ttatttttct ttacagacac tgatattatg
    20461 agctaatttg agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt
    20521 tgcgcatttc ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct
    20581 gccgtttacg tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca
    20641 gatcttctgc tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt
    20701 ctaaatatac atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt
    20761 attcattgac aaatatccga tcaccttttg caaagagctg acgttctttt ttattctcct
    20821 tcctatttgt aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt
    20881 ccgccaagta acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa
    20941 tccacaaagc ttggctttct cctgtttctt ccaacccgca actgatattt aggacgaagt
    21001 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
    21061 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
    21121 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
    21181 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
    21241 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
    21301 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
    21361 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
    21421 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
    21481 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
    21541 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
    21601 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
    21661 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
    21721 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
    21781 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
    21841 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
    21901 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
    21961 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
    22021 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
    22081 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
    22141 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
    22201 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
    22261 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
    22321 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
    22381 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
    22441 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
    22501 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
    22561 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
    22621 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
    22681 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
    22741 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
    22801 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
    22861 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
    22921 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
    22981 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
    23041 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
    23101 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga
    23161 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
    23221 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
    23281 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
    23341 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
    23401 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
    23461 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
    23521 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
    23581 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
    23641 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
    23701 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
    23761 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
    23821 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
    23881 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
    23941 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
    24001 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
    24061 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
    24121 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
    24181 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
    24241 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
    24301 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
    24361 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
    24421 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
    24481 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
    24541 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
    24601 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
    24661 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
    24721 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
    24781 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
    24841 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
    24901 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
    24961 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
    25021 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
    25081 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
    25141 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
    25201 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
    25261 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
    25321 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
    25381 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
    25441 gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
    25501 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
    25561 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
    25621 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
    25681 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
    25741 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
    25801 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
    25861 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
    25921 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
    25981 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
    26041 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
    26101 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
    26161 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
    26221 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
    26281 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
    26341 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
    26401 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
    26461 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
    26521 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
    26581 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
    26641 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
    26701 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
    26761 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
    26821 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
    26881 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
    26941 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
    27001 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
    27061 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
    27121 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
    27181 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
    27241 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
    27301 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
    27361 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
    27421 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
    27481 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
    27541 tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt
    27601 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
    27661 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
    27721 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
    27781 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
    27841 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
    27901 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
    27961 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
    28021 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
    28081 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
    28141 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
    28201 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
    28261 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
    28321 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
    28381 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
    28441 ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc
    28501 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
    28561 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
    28621 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
    28681 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
    28741 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
    28801 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
    28861 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
    28921 ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat agttctcctt
    28981 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
    29041 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
    29101 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
    29161 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
    29221 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
    29281 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
    29341 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
    29401 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
    29461 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
    29521 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
    29581 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
    29641 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
    29701 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
    29761 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    29821 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    29881 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    29941 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    30001 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    30061 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    30121 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    30181 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    30241 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    30301 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    30361 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    30421 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    30481 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    30541 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    30601 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    30661 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    30721 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    30781 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    30841 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    30901 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    30961 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    31021 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    31081 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    31141 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    31201 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    31261 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    31321 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    31381 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    31441 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    31501 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    31561 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    31621 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    31681 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    31741 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    31801 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    31861 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    31921 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    31981 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    32041 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    32101 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    32161 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    32221 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    32281 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    32341 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    32401 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    32461 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    32521 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    32581 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    32641 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    32701 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    32761 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    32821 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    32881 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    32941 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    33001 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    33061 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    33121 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    33181 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    33241 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    33301 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    33361 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    33421 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    33481 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    33541 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    33601 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    33661 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    33721 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    33781 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    33841 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    33901 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    33961 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    34021 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    34081 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    34141 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    34201 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    34261 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    34321 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    34381 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    34441 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    34501 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    34561 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    34621 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    34681 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    34741 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    34801 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    34861 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    34921 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    34981 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    35041 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    35101 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    35161 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    35221 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    35281 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    35341 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    35401 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    35461 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    35521 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    35581 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    35641 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    35701 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    35761 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    35821 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    35881 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    35941 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    36001 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    36061 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    36121 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    36181 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    36241 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    36301 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    36361 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    36421 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    36481 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    36541 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    36601 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    36661 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    36721 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    36781 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    36841 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    36901 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    36961 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    37021 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    37081 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    37141 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    37201 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    37261 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    37321 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    37381 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    37441 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    37501 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    37561 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    37621 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    37681 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    37741 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    37801 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    37861 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    37921 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    37981 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    38041 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    38101 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    38161 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    38221 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    38281 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    38341 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    38401 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    38461 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    38521 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    38581 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    38641 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    38701 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    38761 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    38821 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    38881 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    38941 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    39001 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    39061 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    39121 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    39181 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    39241 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    39301 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    39361 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    39421 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    39481 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    39541 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    39601 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    39661 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    39721 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    39781 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    39841 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    39901 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    39961 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    40021 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    40081 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    40141 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    40201 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    40261 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    40321 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    40381 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    40441 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    40501 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    40561 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    40621 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    40681 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    40741 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    40801 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    40861 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    40921 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    40981 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    41041 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    41101 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    41161 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    41221 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    41281 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    41341 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    41401 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    41461 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    41521 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    41581 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    41641 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    41701 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    41761 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    41821 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    41881 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    41941 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    42001 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    42061 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    42121 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    42181 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    42241 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    42301 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    42361 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    42421 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    42481 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    42541 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    42601 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    42661 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    42721 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    42781 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    42841 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    42901 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    42961 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    43021 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    43081 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    43141 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    43201 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    43261 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    43321 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    43381 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    43441 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    43501 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    43561 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    43621 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    43681 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    43741 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    43801 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    43861 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    43921 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    43981 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    44041 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    44101 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    44161 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    44221 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    44281 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    44341 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    44401 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    44461 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    44521 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    44581 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    44641 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    44701 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    44761 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    44821 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    44881 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    44941 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    45001 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    45061 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    45121 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    45181 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    45241 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    45301 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    45361 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    45421 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    45481 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    45541 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    45601 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    45661 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    45721 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    45781 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    45841 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    45901 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    45961 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    46021 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    46081 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    46141 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    46201 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    46261 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    46321 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    46381 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    46441 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    46501 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    46561 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    46621 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    46681 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    46741 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    46801 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    46861 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    46921 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    46981 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    47041 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    47101 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    47161 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    47221 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    47281 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    47341 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    47401 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    47461 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    47521 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    47581 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    47641 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    47701 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    47761 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    47821 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    47881 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    47941 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    48001 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    48061 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    48121 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    48181 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    48241 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    48301 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    48361 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    48421 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    48481 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    48541 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    48601 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    48661 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    48721 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    48781 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    48841 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    48901 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    48961 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    49021 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    49081 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    49141 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    49201 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    49261 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    49321 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    49381 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    49441 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    49501 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    49561 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    49621 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    49681 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    49741 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    49801 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    49861 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    49921 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    49981 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    50041 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    50101 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    50161 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    50221 cctgagtcaa aatattttcg atgataaaat tgataacgcc catgtt
//
LOCUS       DACDEA010000012        47785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDEA010000012 DACDEA010000000
VERSION     DACDEA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.012"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..2271
                     /gene="acm"
                     /locus_tag="IV136_000708"
     CDS             106..2271
                     /gene="acm"
                     /locus_tag="IV136_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8464718.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2543..3028)
                     /locus_tag="IV136_000709"
     CDS             complement(2543..3028)
                     /locus_tag="IV136_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8464719.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3508..4149
                     /locus_tag="IV136_000710"
     CDS             3508..4149
                     /locus_tag="IV136_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8464720.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4168..4803
                     /locus_tag="IV136_000711"
     CDS             4168..4803
                     /locus_tag="IV136_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8464721.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4821..5636
                     /locus_tag="IV136_000712"
     CDS             4821..5636
                     /locus_tag="IV136_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464722.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5955..7391
                     /locus_tag="IV136_000713"
     CDS             5955..7391
                     /locus_tag="IV136_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8464723.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7580..10027
                     /locus_tag="IV136_000714"
     CDS             7580..10027
                     /locus_tag="IV136_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8464724.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10257..11324
                     /gene="rlmN"
                     /locus_tag="IV136_000715"
     CDS             10257..11324
                     /gene="rlmN"
                     /locus_tag="IV136_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8464725.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11517..12620)
                     /locus_tag="IV136_000716"
     CDS             complement(11517..12620)
                     /locus_tag="IV136_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8464726.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13173..13721
                     /gene="aac(6')"
                     /locus_tag="IV136_000717"
     CDS             13173..13721
                     /gene="aac(6')"
                     /locus_tag="IV136_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8464727.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14267..15928
                     /locus_tag="IV136_000718"
     CDS             14267..15928
                     /locus_tag="IV136_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464728.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15998..16345)
                     /locus_tag="IV136_000719"
     CDS             complement(15998..16345)
                     /locus_tag="IV136_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8464729.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16429..17370
                     /locus_tag="IV136_000720"
     CDS             16429..17370
                     /locus_tag="IV136_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464730.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17371..18414
                     /locus_tag="IV136_000721"
     CDS             17371..18414
                     /locus_tag="IV136_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464731.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18420..19490
                     /locus_tag="IV136_000722"
     CDS             18420..19490
                     /locus_tag="IV136_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464732.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19480..20433
                     /locus_tag="IV136_000723"
     CDS             19480..20433
                     /locus_tag="IV136_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464733.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20445..21455
                     /locus_tag="IV136_000724"
     CDS             20445..21455
                     /locus_tag="IV136_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8464734.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21740..22561)
                     /locus_tag="IV136_000725"
     CDS             complement(21740..22561)
                     /locus_tag="IV136_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8464735.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22658..22936)
                     /locus_tag="IV136_000726"
     CDS             complement(22658..22936)
                     /locus_tag="IV136_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464736.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23204..25213
                     /gene="metG"
                     /locus_tag="IV136_000727"
     CDS             23204..25213
                     /gene="metG"
                     /locus_tag="IV136_000727"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8464737.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25692..26279)
                     /locus_tag="IV136_000728"
     CDS             complement(25692..26279)
                     /locus_tag="IV136_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464738.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26454..27776
                     /locus_tag="IV136_000729"
     CDS             26454..27776
                     /locus_tag="IV136_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464739.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27931..28707
                     /locus_tag="IV136_000730"
     CDS             27931..28707
                     /locus_tag="IV136_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8464740.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28700..29269
                     /gene="rnmV"
                     /locus_tag="IV136_000731"
     CDS             28700..29269
                     /gene="rnmV"
                     /locus_tag="IV136_000731"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8464741.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29262..30146
                     /gene="rsmA"
                     /locus_tag="IV136_000732"
     CDS             29262..30146
                     /gene="rsmA"
                     /locus_tag="IV136_000732"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8464742.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30597..31226
                     /locus_tag="IV136_000733"
     CDS             30597..31226
                     /locus_tag="IV136_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464743.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31350..32459)
                     /gene="ugpC"
                     /locus_tag="IV136_000734"
     CDS             complement(31350..32459)
                     /gene="ugpC"
                     /locus_tag="IV136_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8464744.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32668..33090
                     /gene="mgsA"
                     /locus_tag="IV136_000735"
     CDS             32668..33090
                     /gene="mgsA"
                     /locus_tag="IV136_000735"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8464745.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33413..33949
                     /locus_tag="IV136_000736"
     CDS             33413..33949
                     /locus_tag="IV136_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8464746.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33989..35752)
                     /locus_tag="IV136_000737"
     CDS             complement(33989..35752)
                     /locus_tag="IV136_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464747.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35755..37470)
                     /locus_tag="IV136_000738"
     CDS             complement(35755..37470)
                     /locus_tag="IV136_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464748.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38249..38473)
                     /locus_tag="IV136_000739"
     CDS             complement(38249..38473)
                     /locus_tag="IV136_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464749.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38673..41441
                     /locus_tag="IV136_000740"
     CDS             38673..41441
                     /locus_tag="IV136_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8464750.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            41522..41941
                     /locus_tag="IV136_000741"
     CDS             41522..41941
                     /locus_tag="IV136_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8464751.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41953..42423
                     /locus_tag="IV136_000742"
     CDS             41953..42423
                     /locus_tag="IV136_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8464752.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42440..43219
                     /locus_tag="IV136_000743"
     CDS             42440..43219
                     /locus_tag="IV136_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8464753.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43206..44027
                     /locus_tag="IV136_000744"
     CDS             43206..44027
                     /locus_tag="IV136_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8464754.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44044..45093
                     /locus_tag="IV136_000745"
     CDS             44044..45093
                     /locus_tag="IV136_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8464755.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45106..46110
                     /locus_tag="IV136_000746"
     CDS             45106..46110
                     /locus_tag="IV136_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8464756.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46743..47762
                     /locus_tag="IV136_000747"
     CDS             46743..47762
                     /locus_tag="IV136_000747"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8464757.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15823 a         8305 c         9872 g        13785 t
ORIGIN      
        1 tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg
       61 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
      121 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      181 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      241 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      301 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      361 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      421 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      481 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      541 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      601 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      661 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      721 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      781 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      841 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      901 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      961 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
     1021 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1081 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1141 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1201 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1261 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1321 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1381 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1441 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1501 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1561 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1621 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1681 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1741 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1801 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1861 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1921 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1981 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     2041 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2101 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2161 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2221 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2281 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2341 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2401 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2461 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2521 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2581 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2641 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2701 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2761 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     2821 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     2881 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     2941 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     3001 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3061 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3121 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3181 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3241 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3301 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3361 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3421 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3481 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3541 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3601 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3661 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3721 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3781 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     3841 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     3901 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     3961 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     4021 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     4081 cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat
     4141 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4201 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4261 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4321 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4381 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4441 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4501 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4561 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     4621 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4681 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4741 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4801 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     4861 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     4921 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     4981 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     5041 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5101 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5161 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5221 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5281 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5341 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5401 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5461 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5521 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5581 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5641 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5701 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5761 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     5821 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacataattt
     5881 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     5941 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     6001 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6061 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6121 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6181 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6241 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6301 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6361 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6421 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6481 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6541 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6601 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6661 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6721 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6781 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     6841 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     6901 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     6961 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     7021 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7081 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7141 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7201 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7261 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7321 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7381 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7441 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7501 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7561 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7621 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7681 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7741 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7801 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     7861 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     7921 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     7981 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     8041 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8101 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8161 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8221 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8281 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8341 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8401 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8461 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8521 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8581 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8641 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8701 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8761 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     8821 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     8881 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     8941 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     9001 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9061 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9121 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9181 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9241 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9301 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9361 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9421 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9481 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9541 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9601 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9661 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9721 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9781 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     9841 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     9901 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     9961 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
    10021 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10081 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10141 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10201 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10261 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10321 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10381 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10441 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10501 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10561 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10621 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10681 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10741 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10801 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    10861 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    10921 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    10981 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    11041 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11101 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11161 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11221 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11281 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11341 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11401 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11461 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11521 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11581 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11641 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11701 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11761 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    11821 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    11881 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    11941 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    12001 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12061 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12121 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12181 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12241 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12301 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12361 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12421 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12481 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12541 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12601 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12661 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12721 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12781 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    12841 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    12901 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    12961 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    13021 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13081 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13141 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13201 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13261 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13321 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13381 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13441 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13501 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13561 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13621 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13681 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13741 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    13801 aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa
    13861 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
    13921 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga
    13981 ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca
    14041 ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt
    14101 acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat tattttatga
    14161 aaaagacaaa aaaaagtgtt gattttttaa taattattca ttaaactctt aaacaacttc
    14221 agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt
    14281 tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc
    14341 agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt
    14401 agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt
    14461 tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca
    14521 gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa
    14581 attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta
    14641 tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc
    14701 agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa
    14761 agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata
    14821 tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga
    14881 tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt
    14941 tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa
    15001 agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt
    15061 gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca
    15121 aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt
    15181 caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta
    15241 ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac
    15301 aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga
    15361 agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa
    15421 aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc
    15481 gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt
    15541 caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga
    15601 cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt
    15661 tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt
    15721 taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa
    15781 agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg
    15841 tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga
    15901 ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag
    15961 gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca
    16021 tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac
    16081 cgtagctggc agacgagagc gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg
    16141 caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac
    16201 cgccgataat cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg
    16261 taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg
    16321 cgtaggggat agattttttt ttcatttgat tgctccttta tttttggttc attatctaat
    16381 aatgtagcga aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat
    16441 atcaaatatg tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca
    16501 atcacctttt tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg
    16561 agtcctgaaa caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta
    16621 caatatggga tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt
    16681 aaaaaccagc cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg
    16741 ttacaggcaa ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg
    16801 aagcaaaata catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt
    16861 ccaaactttg tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg
    16921 ccaatcgcaa aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg
    16981 tcgccgcttg ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt
    17041 gattatgtgg aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat
    17101 ggtcttcgaa atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg
    17161 atgaccggat cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc
    17221 gttaagtcaa tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca
    17281 tttatgctga tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga
    17341 attcgtgttt cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca
    17401 gttgcagata tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt
    17461 gaacagatta ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa
    17521 aacaaagctg ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc
    17581 acaatctttg tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct
    17641 cctcgtattc cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa
    17701 cttgttgata aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca
    17761 gacggtttgg gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg
    17821 atcgcattta ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct
    17881 ggtttgttag gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga
    17941 attccaaacc tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca
    18001 atcgtggctg ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa
    18061 acaatgaaat taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga
    18121 atgaaaattg cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg
    18181 atgtttagta ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt
    18241 cttacgcctc cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg
    18301 tatctgcctt atctattatg gattccggca gtaactttat cggttatcat gattggcttt
    18361 aacttacttg ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga
    18421 tgacgaaaat tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg
    18481 tacgagcgat tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg
    18541 gggaatctgg aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca
    18601 acgccaatat agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag
    18661 aaagagagat gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga
    18721 cctctttaga tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata
    18781 ataaagtttc aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa
    18841 tacccgaagc tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc
    18901 aacggatcgt gatcgcgatt gcactggtat gttatccgga aatcctgatt gctgatgaac
    18961 ctacaacagc gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc
    19021 agacaaaaat cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg
    19081 tcgctgatcg tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag
    19141 aaattttcta caacccgcaa catccctata cttggggact gcttggttca atgccgacat
    19201 tagatagtgc gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc
    19261 cgccaaaagg agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac
    19321 aagaaccacc attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc
    19381 cgcaggcacc caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag
    19441 agaaacaaaa acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa
    19501 gttaaaggat tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat
    19561 gacatcagct ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc
    19621 ggaaaatcca ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata
    19681 ttattcgacg gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg
    19741 catgatgttc agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc
    19801 agagatatca ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt
    19861 gcaaaaaaag tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt
    19921 tatcctcatg aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca
    19981 gtaaaaccgc gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa
    20041 gcccaagtgg tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt
    20101 attgcacatg atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac
    20161 ggcttgttat tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat
    20221 acagaaagtt tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga
    20281 agaatcaaat atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca
    20341 ccagaacatt ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa
    20401 cgacaaaagg aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta
    20461 aattccgtat gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg
    20521 ctcgatttac ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag
    20581 tagccaattt ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag
    20641 tagacagtct tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt
    20701 tcaagggacg tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata
    20761 cgaccaacaa ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac
    20821 aaaaagaact aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta
    20881 cgatttctgc tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag
    20941 taaaaaaaga atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc
    21001 aaacatctcg acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc
    21061 aacatgcaca gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt
    21121 tagatcaggc aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac
    21181 aaaagaacca gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg
    21241 actacttatt tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg
    21301 actggcataa attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc
    21361 ttttgatcaa taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag
    21421 aaaaaatggg cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg
    21481 ttagggttag ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt
    21541 tgaagaaaag ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg
    21601 agctttttct gctattgtta attttttatt cagaaagttt acgaatacat caaaattctg
    21661 aaacaaaaaa cgtaactcac aaagagttgt ttttgcgttt tttcttgaat gattaggtct
    21721 gaatcttttg ctcacaacct tattcatggt gccccggctt gagctgatag atttttgaag
    21781 gacggccgat tcctacagga cgttcaccga tctcatcgaa atattggagc atagctttct
    21841 taaaattgga atgatcgatc gttttataat cgatccctaa gaatttagca aatacttttc
    21901 gagcttccgt aatcgtgaaa tctttaccta aaacttgtag aacttgcggc tcgtgttcca
    21961 ttttattgac tacacggttg aatgctttca agatgatttg actatggtca aaagccaaag
    22021 tatctttgcc agaagactct ccagtgacta aatcaagcga gatagccact tctccgctag
    22081 ataaatggat cttgttacta tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat
    22141 ctccggcgat tagcggttct tctccaataa aggctaagta gctgactgtg acgacccagc
    22201 ctcgcggatc tcgattaggc gtactgaatg tatgcagttg ctcaatattt tcttttgaaa
    22261 taaccacacc agtttcttcc tttgtttctc ttaagacgct ttctccagtt gactcatcct
    22321 tttggacaaa gccaccaggc aatgcccatg aatttcgata aggatgccct tttctttgga
    22381 tcaacaaaac tttcagctga tcttcttctt tgttatagca catcagtacg atatcgacag
    22441 tcaaagaggg cttgtcatat tctggacgtt cctgtttttg ataccagtta agaaattctt
    22501 cttcacttgc tttatgttca taatataatt tttcttcttc tttagaagca aaacggttca
    22561 tctgaccacc tccattgaca gtaaaagtac tttttacctt attgtagcag actctgaaaa
    22621 aaaaccaagg atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa
    22681 gtatgagatt gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac
    22741 gaatggcaaa gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag
    22801 ctgctgtcat aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat
    22861 aaaaatagct gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa
    22921 gatgtttctt ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc
    22981 acgtgtgagg ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc
    23041 gcatttattc aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt
    23101 atccagtctt tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc
    23161 aaggaaacga attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac
    23221 tttttatatc actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta
    23281 tactacgatt gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt
    23341 ttatctcact ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg
    23401 tgtaaaaccg caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac
    23461 gcttgatatc agttatgata aattcattcg cacgacagat gattaccata aagcagctgt
    23521 tcaaaaaata tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg
    23581 ctggtactct gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa
    23641 agatgacgaa ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt
    23701 aaaagaagaa tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta
    23761 tgaagaacat cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt
    23821 tatcaaacca ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc
    23881 agtcaagagt gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat
    23941 tactgcacta ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga
    24001 tgttcagatg gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct
    24061 gatggccctt gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa
    24121 agacggaaaa atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg
    24181 ttatggttta gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg
    24241 tgtctttacg ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg
    24301 gaacttattg aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga
    24361 ctatgcctca cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt
    24421 cggacgttac catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg
    24481 ggtgctgatt tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa
    24541 agacgaagaa aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg
    24601 tatcgcagcg atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca
    24661 actaggattg gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc
    24721 aggaacaaaa gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga
    24781 agttgctttt attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa
    24841 atgggatcca gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt
    24901 atttgaaaaa gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc
    24961 agataagttg ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc
    25021 aggaatcgct gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt
    25081 agcaaatctg aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc
    25141 agaagcacca gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc
    25201 agaaatcgca tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc
    25261 gaaatttgag aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata
    25321 cagaaacaga gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt
    25381 ttgttaattt agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa
    25441 agggtcgggc agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat
    25501 aaagtttgaa tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt
    25561 gttaattata agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg
    25621 aaaatataga taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg
    25681 taaaaatcct atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt
    25741 aagagagcag taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg
    25801 ataccataat gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc
    25861 aaggagccag ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact
    25921 ccaaaaggat agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat
    25981 gttccgatat tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc
    26041 ccaaccaaaa aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca
    26101 acgattgcga tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt
    26161 aatccaaata aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa
    26221 aaatcgaccc ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata
    26281 aactgttcct ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca
    26341 tccgtcttaa gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata
    26401 ttctcagtta tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag
    26461 gcaaactaaa acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga
    26521 tcggtgcagg cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt
    26581 tgacattgct agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg
    26641 ctgaattagc tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt
    26701 atggaaaatt gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta
    26761 atattgctgc attatcgatt atctttgcga cacaattgac gaacttatta caattatcta
    26821 ccgattatct tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt
    26881 tagggacaaa agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa
    26941 ttgctgtgat tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc
    27001 caatcgaagc tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa
    27061 cgttgtttgc ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc
    27121 cggaaaaaga tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc
    27181 tattgatcaa ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga
    27241 atgcagcttc tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta
    27301 ttgggatctt gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg
    27361 tgccttatgc gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt
    27421 caaagaagtt tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta
    27481 tgatgagcct tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat
    27541 tcagtttact gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac
    27601 gtccatataa agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt
    27661 tcattttagg tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta
    27721 ccttgatagg tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata
    27781 tttcaacaac agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat
    27841 ttcaataaaa caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg
    27901 ctaaagtaag aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat
    27961 gctgaacagt tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta
    28021 acgaaaatgg cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc
    28081 catgactatc caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat
    28141 acgaaagaaa tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta
    28201 ggggaaattg gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta
    28261 tttcgcagac agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga
    28321 gaagcacttg aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga
    28381 atcatgcata gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg
    28441 catatttcat ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca
    28501 gcaaatgtac caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt
    28561 ccttttagag gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct
    28621 gaacttcgga atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga
    28681 ttatttggtc taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg
    28741 aaaagacgat acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg
    28801 gtctgcaatc aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt
    28861 gatcgttttt accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc
    28921 tgttcctcag gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg
    28981 aagccttggt gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt
    29041 gacgccagac atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta
    29101 tggtttaata gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg
    29161 catcggttac acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc
    29221 gaaagaactc gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa
    29281 ttgcgacacc ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa
    29341 gtttaggaca aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc
    29401 aaatagatga acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac
    29461 aattagccaa gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg
    29521 tattggctga tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa
    29581 agaccgattt gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg
    29641 tagcgaattt accttactat attacgacac cgatcatgat gcattttctt gaatcagatt
    29701 tggttgtgga tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg
    29761 aaccaggaac gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca
    29821 gtttagcatt catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga
    29881 ttttgaaact gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt
    29941 tccgattgac aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc
    30001 attcgtatgg caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg
    30061 gaatcgatcc aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca
    30121 atgcgatgtc agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct
    30181 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt
    30241 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc
    30301 tgccgtttaa ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag
    30361 ttagttggtc ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg
    30421 tgacacaaat tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg
    30481 atactcacac tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta
    30541 agtgagttat actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga
    30601 gaaaaaattc agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta
    30661 tcccaatggt catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg
    30721 tgccattatt tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta
    30781 cgacatttgg tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc
    30841 ttgtgtttgg gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa
    30901 atggacagtt tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga
    30961 ttcattcttt agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg
    31021 aaacatatta ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc
    31081 ttatccacag tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat
    31141 attttgatat ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag
    31201 ggtacgcgaa gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt
    31261 aatggataat aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat
    31321 cttctggcac agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct
    31381 ttatcaaaga agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta
    31441 tggaaatctc ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc
    31501 atcgtttcag ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt
    31561 gaagcttcaa gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact
    31621 tcttttcctt cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc
    31681 ttaagcccat gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg
    31741 gcaggagatc caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc
    31801 ggtgtaccga tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc
    31861 attgcttctg tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt
    31921 ttggcaatct cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca
    31981 tccattaaga agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt
    32041 tgtccaccag ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct
    32101 gctgcattgt ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca
    32161 aaagccatat tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg
    32221 gcaatatcac ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct
    32281 ccttcagaga tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg
    32341 gaaggaccaa caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca
    32401 gaataatttt ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata
    32461 atacgttcca cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt
    32521 aactaacttc aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt
    32581 ttaagcttta ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg
    32641 ttaacaatat caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa
    32701 aaacctatga tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt
    32761 tttgctaccg ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg
    32821 tttaaatccg gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag
    32881 ttagatttgg tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta
    32941 acagcactta tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca
    33001 gctgaggtgc tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat
    33061 gaaatagaca agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa
    33121 tgttcgccta tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa
    33181 cgtattggat aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata
    33241 tgctatagtt tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta
    33301 agtgctaaga ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt
    33361 ttcgcattcg ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa
    33421 acaccggtta gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac
    33481 tttgtcccga ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc
    33541 acaagtgatc atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc
    33601 tgatttagta ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa
    33661 tgcatttatt gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa
    33721 aaatgccatt ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt
    33781 atggcttgcg ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct
    33841 gttaaaaaca gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag
    33901 tattccttac aaaaaaattt taagaagttt agcgatccac gctaaataaa aaacaagcag
    33961 tcttcccatc ccagaagact gcttgttttt attgtgctgt gaattgtgct tgatagagcc
    34021 gtcggtaata accatctttt tgaagcagct catcatgggt gcccatttcg acaatagctc
    34081 cctgatccat cactaatatc tggtccgcat ttttgattgt ggacaggcga tgggcaatca
    34141 caaaactggt ttttccttcc atcattgcta agaaggcttg ttggattttt ttctctgtca
    34201 atgtatcgac tgagctggta gcttcatcta agatcaacat agggggatta ctgatcatcg
    34261 ttcgagcaat cgtcaacagt tggcgttgtc catctgatat ctttaaccct tgaccgccaa
    34321 tcttagtatc taatttttcc ggcaaacgtt ctacaaattc atacatataa gaggctttta
    34381 gggcatcatt gatttctgcg tcacttgctt ccggattccc aaaacgaaga ttttctcgca
    34441 atgtactgtc aaatagccaa gtatcttgta acaccattcc aaaactttta cgtaatgtat
    34501 cacgttggat agagcggatg tcatggtcat cgactgtaat tgttcctttt gttgtgtcat
    34561 aaaaacgcat cagcaaatta acaagtgtgg attttccggc ccctgttttt cctactatag
    34621 caatggtttg acctggataa gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt
    34681 cataagaaaa agaaacatga tcaaaagaga cttttccttt gatatttttg ggttccaagg
    34741 cataggcatc tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt
    34801 ctagaccaga aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt
    34861 tagaaaactg acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac
    34921 cactcttcaa tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta
    34981 caaaacgaga agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat
    35041 atagatcagc atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt
    35101 tcacgatttt ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc
    35161 tttgctgtct atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa
    35221 taattggtgt agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa
    35281 taaacgaaag aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg
    35341 caatggaaat attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt
    35401 gatcataata acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg
    35461 caacggataa gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt
    35521 tgattaaaac gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct
    35581 gtccttctcc aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg
    35641 caatcccatt gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa
    35701 ggtaaggaat gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc
    35761 ttcctcctgt gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg
    35821 ggtaccctga gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat
    35881 agagcggaca cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt
    35941 acgcaaatcc aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa
    36001 tgctggcttg gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa
    36061 atttttccca ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc
    36121 ttctgcctga gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc
    36181 ccattgaagg ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc
    36241 aatttgctga cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac
    36301 tgtaccggaa ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct
    36361 tccggttgga ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg
    36421 gagagaaaga ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc
    36481 ttcagaagaa aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc
    36541 tagcacctct ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt
    36601 tgaaacaacg atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg
    36661 tccttgttgc aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat
    36721 cccgacattc atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg
    36781 taaataattg ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt
    36841 ctgcgcaaat gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag
    36901 ctgatctagt ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa
    36961 taaaatgatt gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat
    37021 cacaaaagcc atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat
    37081 gaccattgct aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt
    37141 atctgttcca aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg
    37201 attacgtaac tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat
    37261 agcacaaatc agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt
    37321 tcctcggtca ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac
    37381 aagttcgaag acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata
    37441 gtcttttgca taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca
    37501 attgaaaaaa caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa
    37561 gccttttcat aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt
    37621 ttttaaaaaa aagaaaatga gaaaacatgc cagtctcttc attcatagga acgtctattt
    37681 tacttaattt ttctataaat gaattctgca taaaaaatat atcaagtaaa tgtttataga
    37741 tgaaactcta tttacttctt ttttaatttg ttcttgcaat cttttttgct acgtaatttt
    37801 tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta tgaaaatgag
    37861 aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa
    37921 actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca
    37981 agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat
    38041 caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc
    38101 aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt
    38161 tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa
    38221 cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa
    38281 aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa
    38341 taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga
    38401 gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt
    38461 tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga aattccagta
    38521 taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg
    38581 ctgtctataa taagatatat ttttcaattc tttattaaag attttattga caaaatgaat
    38641 ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact
    38701 tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa
    38761 ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa
    38821 atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag
    38881 cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca
    38941 aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg
    39001 acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg
    39061 gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg
    39121 tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta
    39181 attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta
    39241 agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg
    39301 ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca
    39361 cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca
    39421 aaacacgtct cttttttgct acgactgaag agattactag tcatttctta acaactttca
    39481 ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac
    39541 ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt
    39601 cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga aacataggag
    39661 agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc
    39721 ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta
    39781 aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg
    39841 agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt
    39901 tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc
    39961 aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt
    40021 attggacaca atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat
    40081 tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat
    40141 ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc
    40201 agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat
    40261 tagatattga agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga
    40321 aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg
    40381 ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg
    40441 atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa
    40501 acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc
    40561 atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac
    40621 ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag
    40681 aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac
    40741 ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac
    40801 tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc
    40861 aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga
    40921 ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct
    40981 cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga
    41041 aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg
    41101 tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag
    41161 aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga
    41221 gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga
    41281 aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg
    41341 aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga
    41401 gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc
    41461 actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa
    41521 agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc
    41581 attagagctg atctatggga aaaatcattc tattactgta ttcgattgct atactgatga
    41641 gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat
    41701 cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa
    41761 tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc
    41821 tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa
    41881 aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta
    41941 ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta
    42001 gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta
    42061 cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta
    42121 gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat
    42181 aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca
    42241 gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga
    42301 aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa
    42361 atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg
    42421 taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt
    42481 cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct
    42541 gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga
    42601 gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg
    42661 tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt
    42721 actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt
    42781 gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt
    42841 cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat
    42901 ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt
    42961 cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct
    43021 tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc
    43081 ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt
    43141 ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga
    43201 agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg
    43261 tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg
    43321 cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg
    43381 cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga
    43441 ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga
    43501 ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt
    43561 tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag
    43621 gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc
    43681 gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg
    43741 caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg
    43801 gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa
    43861 atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag
    43921 catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg
    43981 gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag
    44041 aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg
    44101 aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta
    44161 gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt
    44221 gatcttactg tcgatattca agaacctttt tattttttca attatggaaa actttctcct
    44281 tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca
    44341 gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt
    44401 ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc
    44461 ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt
    44521 ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc
    44581 aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat
    44641 caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga
    44701 acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt
    44761 gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc
    44821 tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt
    44881 cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt
    44941 ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa
    45001 ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa
    45061 gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat
    45121 gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa
    45181 atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga
    45241 acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc
    45301 tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac aaaagattct
    45361 atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa
    45421 atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct
    45481 cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt
    45541 atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat
    45601 gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca
    45661 tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca
    45721 gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg
    45781 cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa
    45841 gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt
    45901 gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat
    45961 ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat
    46021 gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata
    46081 cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat
    46141 aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta
    46201 taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg
    46261 ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta
    46321 aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac
    46381 taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga
    46441 cagatttaga aatgtccaat ctgtctctta tgtattttaa atcaatactc actaggagaa
    46501 gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag
    46561 gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga
    46621 ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt
    46681 cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat
    46741 ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa
    46801 cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg
    46861 aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga
    46921 ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg
    46981 ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc
    47041 caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag
    47101 ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta
    47161 gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc
    47221 aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa
    47281 ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg
    47341 ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag
    47401 gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg
    47461 gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc
    47521 gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa
    47581 tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg
    47641 ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa
    47701 aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt
    47761 aaatagatga tcaatagtta atcgt
//
LOCUS       DACDEA010000013        47525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDEA010000013 DACDEA010000000
VERSION     DACDEA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.013"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(110..844)
                     /locus_tag="IV136_000748"
     CDS             complement(110..844)
                     /locus_tag="IV136_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8464758.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1171..1581
                     /locus_tag="IV136_000749"
     CDS             1171..1581
                     /locus_tag="IV136_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8464759.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1574..2275
                     /locus_tag="IV136_000750"
     CDS             1574..2275
                     /locus_tag="IV136_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8464760.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2275..3861
                     /locus_tag="IV136_000751"
     CDS             2275..3861
                     /locus_tag="IV136_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8464761.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3880..4101
                     /locus_tag="IV136_000752"
     CDS             3880..4101
                     /locus_tag="IV136_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8464762.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4098..4859
                     /locus_tag="IV136_000753"
     CDS             4098..4859
                     /locus_tag="IV136_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8464763.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5239..5748
                     /locus_tag="IV136_000754"
     CDS             5239..5748
                     /locus_tag="IV136_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8464764.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5823..6362)
                     /locus_tag="IV136_000755"
     CDS             complement(5823..6362)
                     /locus_tag="IV136_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8464765.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="IV136_000756"
     CDS             complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="IV136_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8464766.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7429..8226
                     /locus_tag="IV136_000757"
     CDS             7429..8226
                     /locus_tag="IV136_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8464767.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8254..8889
                     /locus_tag="IV136_000758"
     CDS             8254..8889
                     /locus_tag="IV136_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8464768.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8909..9682
                     /locus_tag="IV136_000759"
     CDS             8909..9682
                     /locus_tag="IV136_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8464769.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10090..10887
                     /locus_tag="IV136_000760"
     CDS             10090..10887
                     /locus_tag="IV136_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8464770.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10865..11278
                     /locus_tag="IV136_000761"
     CDS             10865..11278
                     /locus_tag="IV136_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464771.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11262..13907
                     /gene="mutS"
                     /locus_tag="IV136_000762"
     CDS             11262..13907
                     /gene="mutS"
                     /locus_tag="IV136_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8464772.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13925..16033
                     /gene="mutL"
                     /locus_tag="IV136_000763"
     CDS             13925..16033
                     /gene="mutL"
                     /locus_tag="IV136_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8464773.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16055..16615
                     /locus_tag="IV136_000764"
     CDS             16055..16615
                     /locus_tag="IV136_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8464774.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16700..18688)
                     /gene="treP"
                     /locus_tag="IV136_000765"
     CDS             complement(16700..18688)
                     /gene="treP"
                     /locus_tag="IV136_000765"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8464775.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18911..19624
                     /gene="treR"
                     /locus_tag="IV136_000766"
     CDS             18911..19624
                     /gene="treR"
                     /locus_tag="IV136_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8464776.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19701..20147
                     /gene="msrB"
                     /locus_tag="IV136_000767"
     CDS             19701..20147
                     /gene="msrB"
                     /locus_tag="IV136_000767"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8464777.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20317..20919
                     /gene="ruvA"
                     /locus_tag="IV136_000768"
     CDS             20317..20919
                     /gene="ruvA"
                     /locus_tag="IV136_000768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8464778.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20932..21933
                     /gene="ruvB"
                     /locus_tag="IV136_000769"
     CDS             20932..21933
                     /gene="ruvB"
                     /locus_tag="IV136_000769"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8464779.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21962..22540
                     /locus_tag="IV136_000770"
     CDS             21962..22540
                     /locus_tag="IV136_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8464780.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22592..23074
                     /locus_tag="IV136_000771"
     CDS             22592..23074
                     /locus_tag="IV136_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8464781.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23191..23565
                     /locus_tag="IV136_000772"
     CDS             23191..23565
                     /locus_tag="IV136_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464782.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24243..25595)
                     /gene="brnQ"
                     /locus_tag="IV136_000773"
     CDS             complement(24243..25595)
                     /gene="brnQ"
                     /locus_tag="IV136_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8464783.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25742..26332
                     /locus_tag="IV136_000774"
     CDS             25742..26332
                     /locus_tag="IV136_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8464784.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26460..27809)
                     /locus_tag="IV136_000775"
     CDS             complement(26460..27809)
                     /locus_tag="IV136_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8464785.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27977..28717)
                     /locus_tag="IV136_000776"
     CDS             complement(27977..28717)
                     /locus_tag="IV136_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8464786.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28730..30322)
                     /locus_tag="IV136_000777"
     CDS             complement(28730..30322)
                     /locus_tag="IV136_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8464787.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30890..31186)
                     /locus_tag="IV136_000778"
     CDS             complement(30890..31186)
                     /locus_tag="IV136_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464788.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31362..32087
                     /locus_tag="IV136_000779"
     CDS             31362..32087
                     /locus_tag="IV136_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8464789.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32080..32922
                     /locus_tag="IV136_000780"
     CDS             32080..32922
                     /locus_tag="IV136_000780"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8464790.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32925..33446
                     /locus_tag="IV136_000781"
     CDS             32925..33446
                     /locus_tag="IV136_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8464791.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33699..34262)
                     /locus_tag="IV136_000782"
     CDS             complement(33699..34262)
                     /locus_tag="IV136_000782"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8464792.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34513..34782
                     /gene="rpsO"
                     /locus_tag="IV136_000783"
     CDS             34513..34782
                     /gene="rpsO"
                     /locus_tag="IV136_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8464793.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34970..37096
                     /gene="pnp"
                     /locus_tag="IV136_000784"
     CDS             34970..37096
                     /gene="pnp"
                     /locus_tag="IV136_000784"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8464794.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37606..37872
                     /locus_tag="IV136_000785"
     CDS             37606..37872
                     /locus_tag="IV136_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8464795.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            38016..40007
                     /locus_tag="IV136_000786"
     CDS             38016..40007
                     /locus_tag="IV136_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8464796.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40361..41662
                     /locus_tag="IV136_000787"
     CDS             40361..41662
                     /locus_tag="IV136_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8464797.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41792..43162)
                     /locus_tag="IV136_000788"
     CDS             complement(41792..43162)
                     /locus_tag="IV136_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8464798.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43344..43667)
                     /locus_tag="IV136_000789"
     CDS             complement(43344..43667)
                     /locus_tag="IV136_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8464799.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43992..44600)
                     /locus_tag="IV136_000790"
     CDS             complement(43992..44600)
                     /locus_tag="IV136_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8464800.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44930..45646
                     /locus_tag="IV136_000791"
     CDS             44930..45646
                     /locus_tag="IV136_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8464801.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45659..46354
                     /locus_tag="IV136_000792"
     CDS             45659..46354
                     /locus_tag="IV136_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8464802.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46438..47067
                     /gene="udk"
                     /locus_tag="IV136_000793"
     CDS             46438..47067
                     /gene="udk"
                     /locus_tag="IV136_000793"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8464803.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15408 a         8796 c         9888 g        13433 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaataaa
       61 aaaacttggt acgcaatcgc tgtaccaagt ttttttattt agcttttgat taagccataa
      121 ataataattt atataagaat actgctacga caccagcaca gataggtgct acaactggta
      181 cccatgcata tccccattga gaagaacctt tatgtttcaa tggaagaagt gcgtgtacaa
      241 gacgtggagc aaagtcgcgg gctgggttta gtccaggacc tgtaggacca ccaagtgaag
      301 caactaatgt ccatacaagg aaaccaagtg ctaagtgagc tgtaccaaga ttatcttgga
      361 agaatggtgc ttttgtaata cctagggcac caaagaataa aacgaatgaa ccaaagaatt
      421 cattgacgaa accattgaat ttagaaccaa cgctgttgat tgttgagaat gtacctaaca
      481 catggtttac gtcttctgtt tggtcgtagt aaggtttgta acaagctact acgatcaatt
      541 gtccaatcgc tgcaccaatc aattgtgcaa tgatataagg aacaacttca gcccaaggga
      601 atagaccatt agatgctaaa gcaattgtaa atgctgggtt gatgtggtta ccgctgattc
      661 caccaaaaat cattgctggc atcataaccc cgaatccata cccgacagca atcagcatcc
      721 agtcactgcc atatcctttt gtacctttta aatctacgtt tgctacagca ccgttaccta
      781 gaacgattag taatgctgta ccaatcattt ccgccgcaag acgggtcatt agtgagtaat
      841 ccataaatac ctccaaaagt tataaaaatg caaaaaaaaa agaaacatca acacgaaaag
      901 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
      961 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     1021 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     1081 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     1141 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     1201 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     1261 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     1321 aaaatcggag caactggtga ttttttattg aataatctag ccattttatt tgttcctgct
     1381 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     1441 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     1501 gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa gggtatctgt aaacgaaatg
     1561 gaggagaatc aaattgactg agttttttgt gaatccatta tttggcttaa gtttgacttt
     1621 aggagtgtac gtattatctg acaaattttt gcggaaatat aaactgcctg ttttgaatcc
     1681 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     1741 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     1801 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     1861 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     1921 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     1981 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     2041 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     2101 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctattggta cagcaaaagc
     2161 aatcgagatt ggtgaaatag aaggaatcat ttccggactt gcgatatgcg tcaacggaat
     2221 attgaccgtt ttattgctac cgttattctt tcagcctttt gctggcttgt tttgatgcga
     2281 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     2341 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     2401 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     2461 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     2521 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     2581 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     2641 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     2701 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     2761 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     2821 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     2881 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     2941 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat atatgctagg tgcctttttt
     3001 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     3061 gacaatctgt tgttccagcg attttgccag gtcatggagc cggtatttcc taattatttt
     3121 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     3181 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     3241 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     3301 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     3361 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     3421 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     3481 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatggggcg gtatctgact
     3541 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     3601 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     3661 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     3721 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgact
     3781 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     3841 caaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     3901 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     3961 gttgttctcg tatccgatga tgaatttgat ggctgtccgg tgacgccgta cttattgatc
     4021 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     4081 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     4141 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     4201 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaaatttct ttgcgatttc
     4261 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     4321 tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt actgaataca gcggggatat
     4381 tggatgccta caaacccttg atggaaactg acgaatcgct gtgggatctg atttatgaga
     4441 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccaaa
     4501 aaagcggaac gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga
     4561 tcgcttatac aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt
     4621 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     4681 atgcggctga ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag
     4741 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     4801 aagcaagcta catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac
     4861 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     4921 aaaaaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt
     4981 tttaataatc gttaatattc gaaactaagc ggcttttcac gacctcttga tataaacgat
     5041 attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt
     5101 tttatttgac cattcttatt acttgttgca gggcagcttt ttcactacct tctgcgacac
     5161 ttcagttgga attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt
     5221 gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg
     5281 acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg
     5341 tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc
     5401 ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct
     5461 tctcctcttg gactgataga tgttgcagta ggaagcgtca gcacgctgct tgtattgctt
     5521 atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc cgtgacagca
     5581 tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta
     5641 ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt
     5701 gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa
     5761 tttaaaatag ggcagcatgg aagagactga atgttgagtc ttcttccatg ctgctttttt
     5821 gattaatttg tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct
     5881 gctccagttg ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca
     5941 gttgtaaaag taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca
     6001 agagagccca ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc
     6061 atccaaccag ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca
     6121 aataaaacag ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt
     6181 aaaagataaa ctccaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat
     6241 ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa aaggaatggt catctgagaa
     6301 aggacagaaa taatcgcggc aaattccgca gcaagtatca attctttcaa tgttcggttc
     6361 ataacgccct cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa
     6421 gtgattactt tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct
     6481 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
     6541 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
     6601 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
     6661 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
     6721 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
     6781 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
     6841 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
     6901 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
     6961 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
     7021 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga
     7081 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
     7141 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
     7201 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
     7261 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
     7321 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
     7381 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
     7441 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     7501 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     7561 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     7621 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     7681 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     7741 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     7801 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     7861 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     7921 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     7981 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     8041 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     8101 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     8161 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     8221 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     8281 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     8341 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     8401 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     8461 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     8521 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     8581 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     8641 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     8701 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     8761 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     8821 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     8881 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     8941 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     9001 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     9061 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     9121 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     9181 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     9241 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     9301 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     9361 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     9421 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     9481 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     9541 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     9601 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     9661 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     9721 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     9781 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     9841 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     9901 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     9961 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
    10021 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
    10081 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
    10141 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
    10201 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
    10261 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
    10321 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
    10381 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
    10441 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
    10501 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
    10561 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
    10621 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
    10681 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
    10741 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
    10801 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
    10861 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
    10921 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
    10981 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
    11041 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
    11101 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
    11161 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
    11221 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
    11281 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
    11341 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
    11401 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
    11461 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    11521 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    11581 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    11641 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    11701 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    11761 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    11821 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    11881 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    11941 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    12001 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    12061 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    12121 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    12181 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    12241 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    12301 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    12361 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    12421 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    12481 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    12541 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    12601 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    12661 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    12721 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    12781 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    12841 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    12901 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    12961 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    13021 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    13081 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    13141 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    13201 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    13261 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    13321 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    13381 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    13441 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    13501 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    13561 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    13621 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    13681 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    13741 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    13801 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    13861 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    13921 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    13981 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    14041 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    14101 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    14161 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    14221 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    14281 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    14341 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    14401 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    14461 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    14521 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    14581 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    14641 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    14701 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    14761 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    14821 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    14881 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    14941 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    15001 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    15061 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    15121 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    15181 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    15241 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    15301 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    15361 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    15421 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    15481 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    15541 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    15601 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    15661 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    15721 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    15781 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    15841 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    15901 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    15961 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    16021 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    16081 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    16141 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    16201 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    16261 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    16321 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    16381 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    16441 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    16501 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    16561 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    16621 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    16681 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    16741 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    16801 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    16861 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    16921 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    16981 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    17041 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    17101 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    17161 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    17221 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    17281 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    17341 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    17401 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    17461 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    17521 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    17581 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    17641 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    17701 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    17761 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    17821 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    17881 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    17941 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    18001 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    18061 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    18121 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    18181 gaaaatggaa aattgcttca ccgggtaacc acaaaaaggc attgacacca ttccaaaact
    18241 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    18301 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    18361 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    18421 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    18481 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    18541 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    18601 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    18661 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    18721 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    18781 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    18841 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    18901 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    18961 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    19021 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    19081 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    19141 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    19201 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    19261 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    19321 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    19381 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    19441 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    19501 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    19561 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    19621 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    19681 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    19741 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    19801 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    19861 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    19921 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    19981 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    20041 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    20101 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    20161 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    20221 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    20281 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    20341 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    20401 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    20461 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    20521 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    20581 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    20641 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    20701 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    20761 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    20821 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    20881 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    20941 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    21001 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    21061 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    21121 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    21181 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    21241 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    21301 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    21361 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    21421 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    21481 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    21541 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    21601 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    21661 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    21721 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    21781 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    21841 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    21901 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    21961 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    22021 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    22081 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    22141 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    22201 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    22261 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    22321 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    22381 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    22441 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    22501 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    22561 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    22621 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    22681 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    22741 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    22801 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    22861 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    22921 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    22981 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    23041 gattgatgaa aataaaataa caaacaattt gtaaagttaa cgctttcttt ttaaggcgat
    23101 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    23161 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    23221 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    23281 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    23341 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    23401 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    23461 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    23521 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    23581 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    23641 gctgatatca gtaaaacgga acgttttagg atgagtatca cagcatgaca gcattgggtt
    23701 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    23761 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    23821 tgcttatata atagtcacta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    23881 agtttttttg cgaaaactat ttttatatca tatatatgtc taaaatctat attataaaag
    23941 aaatgttgtt tgatcagctt ctcttcggta ttagttcaaa acaaaacggc aaagtatatg
    24001 gagctacttt gccgttttgt ttctctaata ctcaggtcag tttatagacg ctcaataaac
    24061 ggtgttcaaa agccagtttc tcggtattaa gtcaaattta tgcaaaaatg tgagaagcca
    24121 ttttcaaaaa atatttctta atactcgaaa ctgagcggct ttttcacgac ctctcaataa
    24181 acagtgttca agagacagcc tatcggcaat aaggctgaac gtctctttca caacctcttt
    24241 tatcaatctg ctacttcttc tttcttagcg aaactccaaa tcagtccgat aacaaaaccg
    24301 ataactgctg gtacgatcca ccccatcccc aatttgaaaa acggcaagta gctttccgca
    24361 acatataaaa gattttgaac aataggtgtt tgtttgatag tttctgggca agcgttcaac
    24421 ccatcaaaaa tcgaagcaat caatgtaaaa taagtagtca ttcgataaac agccgtacgc
    24481 tgcttaaaca aaggaccaac taacactagt aaaatcagtg tcattgctaa tggatagatg
    24541 aacatcaaaa caggcaacga aaattgaatg atattcgtca agcctacgtt tgccgctaaa
    24601 caaggaagga tactagcaag tgcaacaaag aatgcgtaat ttctttgcgg aaacaaatct
    24661 gtgaatgttt ctgcaaaagc tgtaatcaaa ccaataccgg tcttcaagca agctaatata
    24721 acgataaatg ctaataagat actgccataa gtacctaaat aatgatcagc aatctgagcc
    24781 agcgtgatcc cgccattttc acttagagca aagctaccta agctcatagc acctaaataa
    24841 gcgagcaaag tataaatgat tcccatcaat ataatgctga tcgcaccaga tttgatcgtg
    24901 tctttagcga tttcagcagg ttttgttaca cccatgtttt ggattgttgt gacaatgatg
    24961 atcccaaatg ctaaagcagc aagcgcatct aatgtgttat atccttgagt aaaccctgtg
    25021 aaaaaagcat gctcttgata atttggctga ataggcgctt gatttacaga acctagtgga
    25081 ttcaaaaaag caaggagaag caaagcacct aataaaacca aaaatgcagg attcaggaac
    25141 tttccaacat aatctaatat ctttgtcggt tttcttgaaa accaccaagc cagcacaaaa
    25201 aatacaatac tgaaaattgc tagatatagc gtctgtctgc taggatcgat aaatggtgct
    25261 aaaccaatct cgaaagatgt ggttgcaagg cgaggtaaag caaaaaacgg tccgataact
    25321 aagtaaagca agatggtaaa aaccatcgca tatgtttgat ttatccgttt tgctaattca
    25381 tatacacctg agcttttcga tacaccaatg gcaatcaccc ctaagaaggg aagaccgata
    25441 ccggtaacca agaaacctag attcgcccaa aaggtagcag cgcctgcttc ttgacccaaa
    25501 tgaacgggaa agataagatt tccagcacca aaaaatagac cgaaaagcat ggagcctata
    25561 aaaagatagt ctttaaatga taattttttt gtcatacata actcctttac ggttttttta
    25621 aaagtaacaa aatttcgaaa aatatgcaac aattttcaga aaatttccta tataaaacga
    25681 cggttattta ataacgtaag tgataaaata aaagcgctga atcatctgag gaggaatcat
    25741 cgtgacaaag tcgagattag aagcatttac tgatggagtg gtagccattg ttttgagagt
    25801 tcttgtatta gagatcaaga tccctaatac atctagcttt cattctttat gggtcatccg
    25861 aaatacatta ttagcttata ccatcagctt tatatttgtt gcggtgatct gggtgaacca
    25921 tcatcgtata tttcagatgg cagaacgaat taattaccga gtggtctggt cgaatatctt
    25981 tttgctcttt tggctgactt tatgtccttc tgttactagt tgggtcgggc gtaaccctga
    26041 agctttttgg cctgagatgg cttatgtctt agtatacacg atgtggagtt tttcttttgg
    26101 tatcttgatg aggcaaatca tgaaagcaaa taaaccagat tcccatgtcg tgagagtttt
    26161 gtcaaaggac cgtcgaagtc ttatctccat gatgattaac ttatgcttga ttgccggtgt
    26221 cttttttgta ccgttgatcg ggttattcgg acgttttttt gtatctggta tttggatttt
    26281 ttcctataaa aaagcagatg cctattatca gcggttgttt ccggggaaat agaaaagagg
    26341 ctgggacaga agcttttaac tccaagaaat aagccgaaat ttcacaaaaa tttaagaagc
    26401 aattttcgta aattccttct tatttctcga agctgaccac ttctgtcaca actactttct
    26461 tacttcttac gttcatattt catatgatag attagtaata gaagcaagaa ccacacaggt
    26521 gttagtaata aagcgattct cgtatctttt cctaggaaca agacgacaca cacaaaggcc
    26581 atgtaaagaa ataccagata attactaaat gggtacaacg gcattttaaa tgaatgagct
    26641 tttttgccga ggatactttt acgatatttc atgtgcgtgt agacaagcat actccagata
    26701 aacaagaaac aagtagtggc aatactgctt actagtgtga atacttcact aggcatcacg
    26761 taattcaaga taacagaaat gaaaatgacg atagtagaaa atgtcaaagc attccctggc
    26821 acatgatgtg ttgttaactt tttgaatttt ttaggggcac tgccttcttg cgctaaagaa
    26881 cgaagcatac gtcctgtact atatattgca ctgttgcagg cagaagctgc tgcacttaat
    26941 acgactaaat tgatcaagga agcagcaatc gtgatcccaa tttccgaaaa tacttctaca
    27001 aatggactgt tatctggatt caagctgttc catggataga ttgccataat gacaaaaaga
    27061 gatcctaagt aaaataagat gatcctgata ggaatattat tgatagcttc cggcaacact
    27121 ttttcaggat tttctgtttc tccagcgact aatccaacta attctatccc agcaaaagca
    27181 aaggttacca tttggaaagc taagataaat ccttttgcac cagttggaaa gaatccgcca
    27241 tgagaccata gattcgtgat actggctgtg ccaatatctg tcgtatagtg ggtgaggatc
    27301 atataagctc ctacgaggat aaaggcaatg atcgctacaa ctttgatcaa tgcaaaccaa
    27361 aactctaact caccaaacaa tgagacagcc accatattca agcaaagaag aagacctaat
    27421 gcaatcagtt caggaagcca ctgaggaaca ccaggcgccc agtagcgtac atacattccg
    27481 atggctgtta aatcggccat tgcaatcgat acccagcaga accaatacgt ccagccagtg
    27541 atgaaagccg cttttttgcc aagatattct gcaacaaaat ccagaaaaga atggttattg
    27601 gtgtttgata acagaagttc gcctaacgca cgcataatga aaaaacagat gcatccagta
    27661 ataagatagg cgagcaatat cgaagggcca gctagctgga tagactttcc ggaacctaaa
    27721 aacaaccccg ttccaatcgc tccgccaata gaaatcagtt ggacatgtct atttttcagt
    27781 ccacgctgca gttcttgctc ttcattcatt tttaaatcat cctaactaaa aaagtttaat
    27841 tacttccata ttaaaagagt tttctgaaaa acacaactaa gattccaaaa ttttcagaaa
    27901 tttacctttt atgagtaatt cataaaaaag aactgcggta aacaccgcag ctctttgtcc
    27961 caatcagcta ttgatcctaa gcatttaaaa tacgtgaaag gaattctttt gtacgttctt
    28021 ctttcggatg gacaaagata tcttctggtg taccttcttc tgcaatcaca cctttatcca
    28081 taaaaatgac acggtcagat acatcttttg caaattccat ctcatgcgtc acgataagca
    28141 ttgtcaagcc agtatgtgct aagtctttga tcgtattaag cacttcgcca accatttcag
    28201 ggtctaacgc cgaagtaggt tcgtcgaata gcaatacatc tggattcatt gataatgccc
    28261 gagcaattgc gacacgctgt ttctgtccac cagataattg tgctggtttg gcttcgatat
    28321 aacgatccat accaactttt tccagatttt cgattgctac ttttttggca gtttctttat
    28381 ctcgtttcag gacagttgtt tgaccagaca tacagttttc caagacattc atattgttga
    28441 acaggttgaa ggattggaaa accataccta gattcgttcg atatttaggt aaattatatt
    28501 taggagctaa tacattttct ccattataaa tgatctcgcc gcctgttggt ttttccagta
    28561 aattgataca gcgcaagaaa gtagatttac cagaaccgga agaaccgatg atcgtcacaa
    28621 cttcaccttt attgactgtt acgctgatat ctttcaatac ttcattttca ccgaagcttt
    28681 ttcttagatg tttgatttca ataatttttt cattcatgat tcttcctcct tattcctgca
    28741 aatttgaatt gtcgatttct tcaagcttgg tataagctga aggaccatcg attttctttt
    28801 ctaccgcccg caggattctg gtgatcgtaa aggtcatgac taagtagatg atcccgacga
    28861 tcgtaaatgt ttggaagaat tggaaattcg caccggaagc agagtttcct tggaagaaca
    28921 aatcagctac gccgattaca cttaaaactg ctgtatcttt gatattgata acaaactcat
    28981 ttcctgttgc tggcaagata ttccgtaaaa cttgtggaat aacaactttt ctcatcgttt
    29041 ggccatgggt cataccaatg gcttgcgcag cttcgaactg cccttcatct actgcaaaaa
    29101 tccccccacg aacgatctct gtcatgtaag ctccagtatt gagagagaca ataaacaaag
    29161 cggcaatcgt tcggttcaat gaaatgccaa aagcgagtgc cagaccgtag aagatgacca
    29221 ttgcttgtac catcatcggt gttccgcgga atacttcgac atagatagaa agaagtgcat
    29281 ttccgatttt ttggaagaag cgagtcagtg catttcctga ttcaggtacc gaacggaaga
    29341 caccaatcag taatccaagt gtagtaccga caactgtccc gatcaatgcg atgaacaagg
    29401 taagtccagc acctcgtaag aacatattgc cgtattgttt ccaaatcgta gtgaaatcag
    29461 aaatgatgcc aggtttttct tcactatcag ttgcagcagg ctgatctttg actgcttggt
    29521 ccataatttt ggttcgttca tcgttagaga tgccagaaag aatctggttt actttttgga
    29581 tatccgggtc gcctttacgc ataccaacag aaatctgaac atcttccggg tttgtctgga
    29641 agccatctgc accgctgaat tcaagcattt ccaagtcttt gttcacacta gttgccgtta
    29701 caccttccgg acgttcgctg acataaccat cgatcattcc agaagctaaa gctgttctca
    29761 ttgctgagaa attatccatt gcttgttgtt tctgcacatc tggtatctga tcgatcacag
    29821 tatagtggaa tgtatttaac tgagcagtaa tctttgctcc agaaagatct gctaaacttt
    29881 ttgcttttgc atatttgcta tctttttgaa ctactactac taattgtgat tcgtaataag
    29941 gatcggtaaa atcaatttct tttcgtcgtt cagcagtagg gctcattccg gcgatgatcg
    30001 cgtcgatctt gcctgattga agggcaggag ccagaccatc ccatttcgtt tggacgatga
    30061 ctaatttttt ccctaaacca tctgctactt ttttagcgat ttgaacatca taaccgccgg
    30121 cgtaactatt ttggccttga attggcaagg cattattctc atctgtttgt tgagaccagt
    30181 tgaagggtgc atacccagcc tccataccta cgcgaaactc atcgctcggt gttttttcat
    30241 ctgcatttgc cagagatccc acactgaata gtgagaagac aaaagcaaat agtacggcaa
    30301 ctgaaaccat attttttttc atttttttct ccctttctga ttatatgata cacgagcggt
    30361 ctagatgaaa tgcggtaaag ctttatctga aaaaataaaa agcgaccgat ttatagatat
    30421 aaaaaggccg ctaaaattcc ataaggtgat aaacctctca caaaacatag cgcaacttag
    30481 catcactgct aagacagtcc gcaagctctt aacttgcgtc ccaacataca ctccaagcaa
    30541 aaaagtgcac atttcggcga tcctccctat tcctgcttct ccatgtttgc ttcactccac
    30601 agggttaatg attctcgcga cctctacctc attgattgag gcatctcgta ttcagttatt
    30661 tatacaaaaa aagtttacga tactcgagag gctttgtcaa tctataactg ctgttttttg
    30721 aaaaaatact gataaatata gaaatcttta cttttaccga aaaaacaatc aaaatgtgaa
    30781 tcatctatcc attgcaaacg atagtataat tatacacaaa aatgtatgta atacaataaa
    30841 aaaagaacta gaacagaaaa tttcctgtgt ccagttccat ctcgatcatt taagctgccg
    30901 ctttctttgg ctcacgtcct aggatcgctt gcaaaagaac aacgacgcca taaatggcta
    30961 atattagata aatcaaactt gagtattggg tgattcccca gccccatgtt cgatatacgg
    31021 ctaatgtaat ggctaaaaca attgcggcaa tcacgttgaa taaagcaaaa gcccaaagat
    31081 ttccattttc tttgcgtaca agataaaaaa cagagaaata aacgcttaac gctaaccagg
    31141 cgatacaagc cagaataacg ccccaataaa taaagaatgg taacatatcg attcacctca
    31201 cttttataca gcagtattgt aacacgtttt cattcttttg tcagtgaaca ttgtgataaa
    31261 ttgttcaaat gcccataaca cttttttatt cccatttttc tgacttttca acaataaatt
    31321 tgttcgagta tgggctataa tggaaagtga ggtgacaaaa catgaaacaa ttaacagtag
    31381 acgaagcagc aaaagagata ttagcttatc agcaaattac atttttgacc ggtgcagggg
    31441 tatccacacc ttctggagtg ccagactatc gatctttgtc aggaatctac cacgggatgg
    31501 agcagccgga atacttgctg agtcatcaag caatggtgaa tgaaccggat aaattctatc
    31561 aatttgtcaa aaagatttat catcctgaag caaaacccaa tatcattcat ttggaaatgg
    31621 cacatctagc gaaagaaaaa aatgtttggg ttgtttccca aaacatcgac ggtttgcacg
    31681 caaaggctaa aagcccgcaa ctcgtcaatt ttcatggaag tttgtatcaa tgtcattgtc
    31741 gaaaatgtca acaaacggta gactggaaag aatatcttca cagcgacaaa catcaagcat
    31801 gcggaggaca aatacgccca gacatcgtgt tatatggtga agggtttcaa gatggagtca
    31861 tggagcaggc ggcttatgcc gtcagccaag cagagctgat cgtcattgtt ggaacgagtt
    31921 ttcaagttca tccgttttgt gatctggtac agtttcgcca gccgtcagcc aaaatcttag
    31981 taatcaatca aacgcctgtt tatttagcgg agccttatac gtttattcaa gaagacggag
    32041 cggttgtttt caaaaaaata caagaattag gagcagatta tgactgaatt caaaaccgaa
    32101 aaagaaaaaa tgattgcagg cgagctttat tttgccaatg atcctgagct ggttgccgac
    32161 cgtatgtttg caagaagtca aagccaaatc atcaaccaag cagaatcagc ggaactgcga
    32221 tcccaattgt tgaaagagac ttttggccgt acagggaaaa aaatctatat ggagccagtt
    32281 atcaattttg attacggata taatatattt gttggggaga atttttatgc aaatttcaat
    32341 tgtacatttt tagatgtcag cacgattgaa atcggtgata actgcatgtt cgctccgaat
    32401 gtccagcttt acacggcgac gcatccacta catccagtga aacgtaacag cggtctggag
    32461 tacgcaaaac ccattaaaat cggtaataat gtttggttag gcggaggagt tatcgtcacg
    32521 ccgggtgtca ctctagggga caacgtagta gtcggcgcag gttcggttgt gaccaaatct
    32581 tttcccgaca atgtagtgat tgcaggaaat cctgctcgta tcatcaagac agtagaggaa
    32641 gaactaacag aagagtctct tgaaacatta cgtcataaga tcgatatgat cgatcgagag
    32701 ttgattgctt tattagaaaa aagaatggat acggtaacta aaataggaca agtgaaaaaa
    32761 gaagaaaacc aagcagttta tgatgcaaaa agagagcagc aagtattgga taaagttgtt
    32821 agtctgctga aaaataaggg atataaggaa acaataacag atacgtatgt tgatttgatg
    32881 aaacactcgc gcgaatacca aaacaaaatg aaggaggaat aagtgtgatc gaaggagaga
    32941 cacggcgaaa acaaatcatg caaaccttgg caaatacaga aaaaccagtg agtgctagca
    33001 agtttgcgaa aacttttgga gtcagccgtc agatcattgt aggcgatatt gctttactac
    33061 gagctgcagg taaaagtatc gtggcaacag caagaggtta tatcctcgaa acagaagaaa
    33121 ccaatactgg gcacatcagt aaaatcgctg tccagcatgg aaaagatcaa acagaagaag
    33181 aactgagact aatcgttgaa aatggtggtg aaatcattga tgtaattgtg gaacatcccc
    33241 tttacggtga gttagcagga actttgcaca tcaaaacgcc acaagatatc gattccttta
    33301 tgagacgtta tcaaaaaagt aatgctactt tgttatccga attaacaggc ggcatccatc
    33361 tgcatacgat ccgctaccca gaaaagcata tgctgaaaaa catcaaacaa aaactagctg
    33421 aagcaggaat cctctacgaa ggataacatc gtagctgcta ctttttatcc tagaaactat
    33481 tctatcatct ctgagataaa cacccatatg gaagaaataa gaaaaaataa gaaaagatgc
    33541 gtatccaata cacagaattt tttggaattg cgatttctag atgtctatct aaatttcgat
    33601 tagtggagaa acataaaaaa gggaagtgca gcttttgagc actccccttt tttataaagc
    33661 tttcttgtag catagtatcg aaggaacctg ccaattctct actcgatcac caatacgcct
    33721 tcttttagtg caaatggatt ttgatcgttg atgtggtcat agaacatcac accgttgata
    33781 tggtcgattt catgttgaac gacgatcgat tcataattct tcaaacgaat cttgtgtttt
    33841 tcaccattca tatcataata agacacagtg attttggcat ggcgaacaac atatcctggt
    33901 acttctcgat caacagataa acagccttca ccttccccta aacaagcatc ttgaacagaa
    33961 tggctcaaaa ttttaggatt atacataaca gtacttagag aaggtccatc tgcctctggg
    34021 tcaggacttg gtacgtgaac agcaatgatt cttttagaaa tatctaattg aggagcagct
    34081 aagccaacac cgcctcgtaa gtgtagttct tcagctttga ttggatcttg gctgtttttt
    34141 aagaactcaa gcatctcttt tcctaaagag atatcttctt cgcttaatgg aagagaaact
    34201 tcttttgcaa cttcgcgtaa ggtcggattt ccttcacgaa tgatatcatc cattgtaatc
    34261 atggaatatg tacccctttc ttaaaagact gtgtttcaag tctattttcg cattatagtt
    34321 taccacaaaa atggaaaagt agtagtaaga ttgtttgaaa actacctttt cttaatgatt
    34381 aactacttga ttttcaaccc ggtttcaggt aaagtatagc tgtgtgctct aggcacatga
    34441 cctgccactt ggttaagcga atcaccaacg ttttactcag tcgcagggga ataatcgaaa
    34501 gaggtgaaag aaatggcaat ttcaaaagaa cgcaagaatg aaatcatcaa cgaatacgct
    34561 cgccatgaag gggatactgg ttctccagaa gtacaaatcg ctgtattaac tgaagaaatc
    34621 aaccacttga atgaacatgc acgcgtgcac aaaaaagacc atcattcata ccgcggccta
    34681 atgaaaaaag ttggtcaccg ccgtaacttg ttagcttact tacgtaaaac tgacgttcaa
    34741 cgttaccgcg aattgatcaa acgtttaggc ttgcgtcgtt aattttagtc aaaaagagtg
    34801 ggattcacat gaatctcact ttttttactc gtatatcaat taagtaaaat tccgtgatca
    34861 agctagatgg actcctacta accaaatgaa agctgctcaa aagacggctt tcggcagttt
    34921 tcatttgttt agtagcgtct tgctttcacg agaaggaaaa ggagaagtta tgtcagaaaa
    34981 acaagtattc aaaacgacat ggggcggccg tcccttacaa atcgaagtcg gtcagctagc
    35041 aaaacaagca aacggagccg ttttagtccg ttatggagat acagtagtct taagtgcagc
    35101 tgtggcctct aaagaagcaa aagatacaga ctttttccca ttaacgatca attatgaaga
    35161 aaaaatgtat gcagcaggga aaatccctgg aggtttcatc aaacgagaag gccgtccaag
    35221 tacggaagca acattgactg cccgtttgat cgaccgtcca attcgcccga tgtttgcaga
    35281 aggcttccgt aatgaagtcc aagtaacgaa catcgtgatg agtgtagaaa cagactgctc
    35341 gccagctatg gcagcaatgc taggttcttc tttagcacta tcaatctcag atatcccatt
    35401 tgacggccca atcgcaggcg tagaagttgg tcgtgtaaat ggcgagtacg tcttgaatcc
    35461 aacagtagaa caagcagaac aaacagatat cgaattgact gttgccggaa cgaaacaagc
    35521 catcaatatg gttgaaagcg gcgcgaaaga agtttcagaa gaagacatgc ttggtgcatt
    35581 gctgtttggt tttgacgcta tcaaagagtt agttgctttc caagaagaaa tcgttcaagc
    35641 agtagggaaa gaaaaaatgg aagtgacttt actgcaagtt gatgaagtat tgaaaaaaga
    35701 aatctttgat gcttcttacg ctacaatgaa agcagcagta atgactgaag aaaaacttgc
    35761 tcgcgaagat aatatcgagc aggtgaagat cgatatccgc gaagcttatg ctgagaaatt
    35821 tgcaggtcat gaagatgaag atcacttgtt gaaagaagtt aaacaaatca cagaagatct
    35881 tgaaaaagat gtcgtacgtg aattgatcac catcgataag atccgtcctg acgggcgtaa
    35941 attggatgaa atccgtccat tgtcatcaga ggtcagctta ttgccacgtg tacatggatc
    36001 aggtttgttc acacgaggac aaacacaagc tttatcagct tgtacactcg cacctctagg
    36061 agagcaccaa atcattgatg gtttaggggt ggaagtcagc aaacgattca tccaccacta
    36121 caatttccct caattttctg ttggttctac aggacgtgca ggctctccag ggcgtcggga
    36181 aatcggacat ggcgcattag gtgaacgtgc gttagcacaa gtaatccctt cagaagaaga
    36241 atttccttat acgatccgtt tagtagcaga agtattggaa tcaaatggtt cttcttccca
    36301 agcaagtatc tgtgctggca cattagcatt gatggatgca ggtgtaccaa tcaaagcgcc
    36361 agtcgccggt attgcgatgg gacttgtttc tgatggtgaa aactatacga tcctaactga
    36421 tatccaaggg ctggaagatc atcttggcga tatggacttt aaagttgccg gtacaaaaga
    36481 tggtatcact gctttacaga tggatatcaa gattcaagga atcacagaac aaatcctaac
    36541 tgaagcatta actcaagcaa aacaagcacg tatggaaatc ttagaagaat tgacttcaac
    36601 gattgctgct ccacgtgaag aattaagcca atatgcacca aaaatcgaaa tgatccagat
    36661 cgaacctgct aaaatcaaag atgttatcgg taaaggcggc gatacgatca acggaatcat
    36721 cgatgaaact ggtgtgaaaa tcgatatcga tcaagatgga aaagtaagta ttgcttcagc
    36781 tgatgcagaa atgatcaaaa aagctatcaa gatcatcgaa gatttgacca aagaagtcaa
    36841 agttggtgaa gtttatctag ggaaagttgt ccgtatcgaa aaattcggtg cattcgtcaa
    36901 cttgatcaaa ggaaaagatg gacttgtcca tatttcccaa ttagcaaacg atcgtgtcaa
    36961 taaagtcgaa gatgttgtta aactaggtga tgagatcctt gtgaaagtaa ctgaaatcga
    37021 taaacaagga cgcgtaaatc tgtcaagaaa agcaatgttg aacgaagatg gttcaacgaa
    37081 aaatgaagaa aaataattta attcatagga ataatgtact tgtgatcgtg aaggtgacag
    37141 ggggacaata attattcgaa aaaaggggac tgtgacaaaa gccgtgtcac agtccttttt
    37201 tttcgaataa acggtgttca aaagcttgca tctgcggtaa taagtccagc caagttaaaa
    37261 acattaggag ctgtttttat ttggctgttc ttattacttg atgcagaaca gcttttttac
    37321 aacctctcca ataaacgatg ttcaatcagc tgtccctcga tattagctca aaaaacggca
    37381 aaatatgagg aactattttt ccgtttgttt ctctaatact caggtcaaac cgctgatttc
    37441 acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct gaacactttt
    37501 gttacaactt ctttttttct ggagtttctt ggcgcatatt tttggagtcc ttctgaataa
    37561 agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct gaatttttta
    37621 tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt aggaaaggaa
    37681 gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg gctcggcgaa
    37741 tttttactag gaccactggg accggaactc ggcggttttt atttgatacc agcattattg
    37801 ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag agaacgtcca
    37861 aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg gataattaga
    37921 agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca agcttaccta
    37981 aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac aataccaaaa
    38041 agatatcaat gactggcctg cttgtagcga tgggagtcgt ctacggagac atcgggacaa
    38101 gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca ggaatcaatc
    38161 ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta ctgacaacaa
    38221 tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga attttctcac
    38281 tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg atcggaggag
    38341 ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact gcgattgaag
    38401 gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac gtgatcgtca
    38461 ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt acagatattg
    38521 tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc gtaatgggat
    38581 taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat tatgctatcc
    38641 agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat attttcctgg
    38701 ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa cgaaatattc
    38761 gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg caggctgcgt
    38821 ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg aatccattct
    38881 tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca gtcgctgcag
    38941 tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa gcaatcaagt
    39001 tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg caaatgtata
    39061 tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc acattccgaa
    39121 cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg ttgatgacaa
    39181 cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt gcatatttag
    39241 ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt gtcaaattct
    39301 tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg atgtttattt
    39361 gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg agagattata
    39421 tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag acaaatgtgg
    39481 tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc tattcgattt
    39541 tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa gtgacggatg
    39601 agccatatac gaaagaatat caagtagata tgatggacac tgattttatt gtccaagtga
    39661 atctgttctt aggtttcagg gtccaacagg aaatcaacgt atatgttcgc caagtcatcc
    39721 atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct ctgacaccag
    39781 ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc aatgtgacaa
    39841 ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa aaaatcgcta
    39901 cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa actgttccgc
    39961 ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag tagcgctttg
    40021 acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt catgaaaaag
    40081 ctgtactgca acaagcagta agaacgagca gataaaaaca gcttctcatg tttttcatct
    40141 gtttgggctt aatgccgtag atgcaagctt ttgaataccg tttttatcag aggtcatgaa
    40201 aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat gtggctgaaa
    40261 aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca atctttttgt
    40321 agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca atttcattat
    40381 tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg ctttagtgaa
    40441 aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg ccaagctagc
    40501 tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag gaattacgct
    40561 aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac tagaaccagt
    40621 gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg cgctgggatt
    40681 tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga gctatagtat
    40741 cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt tttataaagt
    40801 aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga agctattagg
    40861 tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga cattagtcgc
    40921 attgaattct tatgaaaaag gagagttgac caaagaagaa tattattatt tggaaaacgt
    40981 gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt ccatgcaagt
    41041 attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac aaggacatac
    41101 tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca ccttgccaga
    41161 tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga ttgaagagcc
    41221 gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc gaaaaaaggg
    41281 taagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag tgacgattga
    41341 agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg acgaagcatt
    41401 gatagcggaa cagtcagatg gctcttatat catctccggc aaactaacat tagatgattt
    41461 ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta ctacacttgc
    41521 gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg agattgcatc
    41581 attccgtttc acggtattag aataccagca tgcacatatc gattatttca aagtagagtc
    41641 cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc acagcctttt
    41701 tgttatctaa aaaacctgtt ttcggtaaac gaagaagata ttttcaataa aagttttcat
    41761 ttttagccgc aacgatttct tttaatcaag gctatcttgc ttctgcatgt tctaatgtga
    41821 aatgttcctc ttcacccatg tgtttcaatt gtttcatcga acgcatataa gagtaaagca
    41881 acacggcaac agaaccgatc caagctaaag gagaagctgc tgctgctcct gggtatcccc
    41941 acattcctgt aagaacgata gctgcaaaag aacgcataag caattccatc ataccggcga
    42001 tcgtagggat cttgctttgt ccaagccctt gaagtgtgta gcgtaaaatg aacaggactg
    42061 ctaagatcca gtaaagagac ccattgatat tgaagtaggt ttgtgctaat tcaaatacac
    42121 gtgtttctga tgggttaaca aacaattgaa caaagaaatg accgaagaaa atgacgatag
    42181 cacctgctaa gagactaaat ccgacactca tcagtaaagt ttgacgtacc ccttgcaaga
    42241 ttcggccata ttctttcgca ccatagtttt gtgcagtgaa agttgccatt gcaataccaa
    42301 atgacatcat cggctgattt gcaaattggt cgatacgacc agcagcggct tgagcagcta
    42361 cgacatctgt ccctagacta tttagtgctg cttgtaacac gatagcacca acagcgatga
    42421 tcgaagattg aaacgccatt ggcaaagcag catttaggtg tacagcaaac tcttcttttt
    42481 caaatttgaa atcttttttg cgtaactgta acaaaggcat ttttttcttg atatacacta
    42541 gacacagtac gctggaagaa acttgtgcga ttactgtagc aatagctgca ccttccacac
    42601 ccatgccaaa atagataata aagatcaaat caagtacgac gttgatgatg accgcaatga
    42661 tcagaaagaa tagtggtgtg cggctgtcac caagtgcgcg aatgacatta gaaagcaaat
    42721 tgaaactgac tgatgcaaag atccctaata aaataattga aataaatgtt tgtgcttgat
    42781 caagtatttc aggaggagtt tgcatcaatt gaagcatcgg acgaataaac aggagactta
    42841 ggacagttaa aatcacagtg acgacaagac taatgacaac actagcagca aaacttttct
    42901 tcagaccccg ataatctttt gcaccgtaac gttgtgcggt aataatggct aaaccagctg
    42961 ttaaaccttg ggcaaagcca atgatcaaaa atgtcaggct tccagttgca ccaaccgctg
    43021 ccagcgcatt tttacctagc gtctgaccca cgatgatcat atcgatcata ttgtagaact
    43081 gctgaaaaac atttccgatc agtaaaggaa cagtgaacat caaaatcagt ttagcggggc
    43141 ttccttttgt taaatcgcgc atcttttaaa ctccttatat ctttaaaatt tagttagtag
    43201 tatatagcgt ttatcctgag aataccaatt agaaatcaat gataatttat gaaactccat
    43261 aaaaagaagc caagaattga gtcaaagaat caacaacttg gcctggtctg taaaataagg
    43321 gacatccacg tttatctatc accttaatat tgtctaatct cacttggtaa aaggatcgtt
    43381 tctctcgtac cgtcatcata ggcgtatact tgagcaggaa attggttcga ataggcaaac
    43441 ggcaaatcaa tatccatttc cacattgctt cctgcttgag aaaaatgcat cgtcactttt
    43501 ccacgatttt cgatcaaatc aaaggttaat atattttcta atggaatgac tcccttaaga
    43561 tctaggtcaa taataaacca tatactgtca atcaattcat ctggcaaact tgatacgaca
    43621 ccaactgaag cgtaacggtt gcggctgcta tcaaaacttt caaacatctg tttaacctcc
    43681 ttcgaatcaa cgaatgtttt atttttataa ttaatcctag gataaactag aaatacttac
    43741 ttcaacgagg gttcattcta ataaagaatc aggaaaaatg caacaataat tgaatacttt
    43801 ttttcaaagt ataaattttt ataaaggtac gataaaagta tgcgaggaaa agcttggtaa
    43861 attaggattt ttcgtttgta aagccattta caaaagtgga agccccatat gaaaataaac
    43921 gattagaaat aaaaaaggcg tgttaaaagc tgctgacccg ccgcttctaa caccacctga
    43981 ttgtttcagt attattcttc tgttgaaccg atcacttctg gttcgccacc ttcacctgtg
    44041 ctttcttctt cttcactttg agcgactaca gcagcgattt gctcttcact gtcggtcacg
    44101 aatttgtaat cgtcgttttt tggaagatcg gctactgtaa tagaatcacc taattccaag
    44161 tttgtcacgt caacttcaat tcgttcaggc aatttgtctg gtgtagcaga tacgactact
    44221 gtatacaagt tttgagctaa cactccacca gatttagcac ccactgattc gccaacaaga
    44281 acgacttctg tttcgacttc tgtttcttcc gacatgttta cagatagaaa ttctgcatgt
    44341 tcgaaatgat tggtaaaagt gttcgtttgg atcttgtgaa ccaatgtatt gactttttgt
    44401 ccatcgatgt ccatcgtgat gactgtgttc gctccatgtt cacgcaacaa tttgcttagt
    44461 gtcaaaccat cgaatgagat aggggtactt tctactttat acccataaac aacagctgga
    44521 actttccctt cgtgacgaag tttgtttctt aatgaacggg gacgtacttc tcttttactt
    44581 acttctaatg atactgacat aaccaaaatt cctccttaaa atgtgactgt ctgctttggt
    44641 aacggacaga ttttatttaa gtgaattcta aaggctaatc tcgtgcacat catcgaaggc
    44701 agggcgctta aaaaagcgca aaaggagaga tgcgcgcatc tccccttaga aagtcaggat
    44761 tcaacttttc tccaccaggt cattacgctg tgtatgagaa atccaattca ctttactatt
    44821 taaccgtaac gtgaactgtt aattaagtca aggaataact atgatgatgt atttgcaagc
    44881 aagaatattc aaggtataat taattagtag taataagaaa ggaaacagca tgcgtttaga
    44941 taaattaata gaagaaaaat tagaaacgac acgcaaggaa atgaagcggc tgtttgcttt
    45001 gaagcaagtg atgatagatg gcacagtcga atttcgacaa aatcgaaatg tagatagttt
    45061 actgcatcaa atcgaagtag caggtgaaca cttagaaaca aatgagtcct attatcttct
    45121 caataaacct gaaaaagctg ttacagctgt atcagatcca gaaaagacga cagtaatcga
    45181 tctgattgca agtgccgatc gtttccaacc attatatcca gtgggaagac tagatagaga
    45241 tacaacagga ctattgctga tcacttcgaa tggacaattg ggatatgaaa tgctacttcc
    45301 agaaaaaaag gtttcaaaga cttatcaagt agttgtgaac gaaagggtga caccggcaga
    45361 cgtaacagca tttgaaaatg gaatcgtatt cgctgatggg tatcaatgta agccggctga
    45421 tttaaaaata ctcaaagcag acaagcacca aagtacggtt ttattgacga tccaagaagg
    45481 aaaattccat caagtcaaaa agatgttctt agcccaagga aagaaagtga ccgcattgaa
    45541 acggctaagc atgggaccat tggaattaga tgaacagcta ggagtaggcg agtatcgatc
    45601 gttgacatta gcagagatga aaaagttaag tatttatttt aaataggagg tcaaaaagat
    45661 gaaatataaa actttattgt ttgatgtaga tgatacattg ttagattttc aattaactga
    45721 agccaatgct ttaagtgctt tgtttgcaga acaaggaatc acattgacac cagaaatcga
    45781 aaccagctat aaggctttga atcatcattt atggcaggag tttgaaaaag gcagactatc
    45841 acgagaagaa ataataggca gtcgttttgg gttacttttc cagcaatttg ggaaaacagt
    45901 agatagtgat caaatggaaa aacgctaccg ccactacttg aaccaaggtc atgatcttgt
    45961 tccaggaagt ctagaaatcc tgaaaaaggt ccatccgcat gctgatcttt atattgtgac
    46021 gaacggtgtg tcgcacacgc aacatcggcg attaacagat gcaggaatgc ttgattactt
    46081 caaggatatc tttgtctcag aagctgtcgg agcgcaaaag ccgatgaaag aattttttga
    46141 tcatgtcttt tcccacattc ctaattttga taaaaagcaa actgtgatca ttggtgattc
    46201 tttgacttct gatatcaaag gaggaaatat ggcagggatc gatacgattt ggttcaatag
    46261 aaataaatta ccagaaatcg aagaaatcca gcctacttat cgaatcgatt cattagagga
    46321 actttattca ttattggaaa ttgaaaagga ttaattttca gaaaaatata gcattttcgt
    46381 gttttttcgc tataatagat tagaatgaaa aaacggatac gaaagaggtt ttagtcaatg
    46441 acaaaaagca agccaattat cattggtgtg acgggtggat ccggaagcgg aaagacaagt
    46501 gtcagccgtg cgattttcaa caacttcccg gaccattcca tcatgatgct tgaacaagat
    46561 tcttattaca aggatcagag ccatttgagt tttgaagaac gattgaacac aaattacgat
    46621 cacccatttg cctttgataa tgatctgctg attcagcatg taggcgactt actgaactat
    46681 aaagccattg aaaaacccgt gtatgactat gtagcgcata caagaagtca ggcaacgatc
    46741 attcaggaac caaaagaagt gatcattcta gaaggaatcc tgattttaga agacgaacgt
    46801 ctgcgtgact tgatggatat caaagtatac gtagatacag atgatgacat tcgtatcatt
    46861 cgtcgtatca aacgggatat ggaagaacgg ggacgcacat tggactccgt gatcgaacaa
    46921 tatttgacag ttgttaaacc gatgtatcat caatttatcg aaccaacgaa gcgctatgcg
    46981 gatatcattg ttccagaagg cggagaaaac cacgttgcta ttgatttgat tacaacaaaa
    47041 gtagctagtt tcttaaacca taaatagtgc gcagcgaaac agttgaacgg actgcgaccg
    47101 tttgaaaagc aaggattctg ctttttgaac agtcaccagc caactgtttt tttatttata
    47161 acaagaggtt gtgaaaacag catcttgacc taagtattaa aaagaagaaa cggaaaatag
    47221 ctgtttatat tttaccgttt tttgagctaa taccgaaggt cagctgattg agcaccgtga
    47281 agtagaggtt gtaaaaaagc tgtctttgca tcaagcgata agagcatcca aataaaacag
    47341 ctcctcacgt ttttcgttgg gttgggctta ttatcgtaga tgcaagcttt tgaacaccgt
    47401 ttaacgagag gttgtaacag aagtggtcag atgcaataaa taagaagaaa cttacgaaaa
    47461 ttatttctcg aatttgtgtg agatttcgac ttattttttt gaagaagctc ttttgtcgca
    47521 ccccc
//
LOCUS       DACDEA010000014        44836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDEA010000014 DACDEA010000000
VERSION     DACDEA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.014"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..243
                     /locus_tag="IV136_000794"
     CDS             4..243
                     /locus_tag="IV136_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464804.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..519
                     /locus_tag="IV136_000795"
     CDS             310..519
                     /locus_tag="IV136_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464805.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            579..1250
                     /locus_tag="IV136_000796"
     CDS             579..1250
                     /locus_tag="IV136_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8464806.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1303..3279
                     /locus_tag="IV136_000797"
     CDS             1303..3279
                     /locus_tag="IV136_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8464807.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3292..4047
                     /locus_tag="IV136_000798"
     CDS             3292..4047
                     /locus_tag="IV136_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8464808.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4044..4868
                     /locus_tag="IV136_000799"
     CDS             4044..4868
                     /locus_tag="IV136_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464809.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5157..7268
                     /locus_tag="IV136_000800"
     CDS             5157..7268
                     /locus_tag="IV136_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8464810.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7306..9363)
                     /locus_tag="IV136_000801"
     CDS             complement(7306..9363)
                     /locus_tag="IV136_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8464811.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9477..9689)
                     /locus_tag="IV136_000802"
     CDS             complement(9477..9689)
                     /locus_tag="IV136_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464812.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9876..10343
                     /locus_tag="IV136_000803"
     CDS             9876..10343
                     /locus_tag="IV136_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464813.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10340..10582
                     /locus_tag="IV136_000804"
     CDS             10340..10582
                     /locus_tag="IV136_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464814.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10600..10902
                     /locus_tag="IV136_000805"
     CDS             10600..10902
                     /locus_tag="IV136_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464815.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10974..13028
                     /locus_tag="IV136_000806"
     CDS             10974..13028
                     /locus_tag="IV136_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464816.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13028..15640
                     /locus_tag="IV136_000807"
     CDS             13028..15640
                     /locus_tag="IV136_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8464817.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15637..18168
                     /locus_tag="IV136_000808"
     CDS             15637..18168
                     /locus_tag="IV136_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464818.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18220..18552
                     /locus_tag="IV136_000809"
     CDS             18220..18552
                     /locus_tag="IV136_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464819.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18553..19146
                     /locus_tag="IV136_000810"
     CDS             18553..19146
                     /locus_tag="IV136_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464820.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19309..21003
                     /locus_tag="IV136_000811"
     CDS             19309..21003
                     /locus_tag="IV136_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8464821.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21691..23568
                     /gene="ltrA"
                     /locus_tag="IV136_000812"
     CDS             21691..23568
                     /gene="ltrA"
                     /locus_tag="IV136_000812"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8464822.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23699..24076
                     /locus_tag="IV136_000813"
                     /pseudo
     CDS             23699..24076
                     /locus_tag="IV136_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24076..25281
                     /locus_tag="IV136_000814"
     CDS             24076..25281
                     /locus_tag="IV136_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8464823.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25297..25941
                     /locus_tag="IV136_000815"
     CDS             25297..25941
                     /locus_tag="IV136_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464824.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25952..26758
                     /locus_tag="IV136_000816"
     CDS             25952..26758
                     /locus_tag="IV136_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464825.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26780..27010
                     /locus_tag="IV136_000817"
     CDS             26780..27010
                     /locus_tag="IV136_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464826.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27556..27831
                     /locus_tag="IV136_000818"
     CDS             27556..27831
                     /locus_tag="IV136_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464827.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27873..28295
                     /locus_tag="IV136_000819"
     CDS             27873..28295
                     /locus_tag="IV136_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464828.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28314..29906
                     /locus_tag="IV136_000820"
     CDS             28314..29906
                     /locus_tag="IV136_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464829.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29918..30253
                     /locus_tag="IV136_000821"
     CDS             29918..30253
                     /locus_tag="IV136_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464830.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30400..30594
                     /locus_tag="IV136_000822"
     CDS             30400..30594
                     /locus_tag="IV136_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464831.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30626..32998
                     /locus_tag="IV136_000823"
     CDS             30626..32998
                     /locus_tag="IV136_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8464832.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33059..34519
                     /locus_tag="IV136_000824"
     CDS             33059..34519
                     /locus_tag="IV136_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8464833.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34530..36734
                     /gene="topB"
                     /locus_tag="IV136_000825"
     CDS             34530..36734
                     /gene="topB"
                     /locus_tag="IV136_000825"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8464834.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36973..37566
                     /locus_tag="IV136_000826"
     CDS             36973..37566
                     /locus_tag="IV136_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8464835.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37582..38481
                     /locus_tag="IV136_000827"
     CDS             37582..38481
                     /locus_tag="IV136_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8464836.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38484..38969
                     /gene="ssb"
                     /locus_tag="IV136_000828"
     CDS             38484..38969
                     /gene="ssb"
                     /locus_tag="IV136_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8464837.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39335..39595
                     /locus_tag="IV136_000829"
     CDS             39335..39595
                     /locus_tag="IV136_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464838.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40036..40242
                     /locus_tag="IV136_000830"
     CDS             40036..40242
                     /locus_tag="IV136_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8464839.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40242..40493
                     /locus_tag="IV136_000831"
     CDS             40242..40493
                     /locus_tag="IV136_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464840.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40508..40945
                     /locus_tag="IV136_000832"
     CDS             40508..40945
                     /locus_tag="IV136_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464841.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40938..41645
                     /locus_tag="IV136_000833"
     CDS             40938..41645
                     /locus_tag="IV136_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464842.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42026..42205
                     /locus_tag="IV136_000834"
     CDS             42026..42205
                     /locus_tag="IV136_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464843.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42546..42809
                     /locus_tag="IV136_000835"
     CDS             42546..42809
                     /locus_tag="IV136_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8464844.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42803..43153
                     /locus_tag="IV136_000836"
     CDS             42803..43153
                     /locus_tag="IV136_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8464845.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43177..43791)
                     /locus_tag="IV136_000837"
     CDS             complement(43177..43791)
                     /locus_tag="IV136_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8464846.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44105..44527
                     /locus_tag="IV136_000838"
     CDS             44105..44527
                     /locus_tag="IV136_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464847.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44537..44740
                     /locus_tag="IV136_000839"
     CDS             44537..44740
                     /locus_tag="IV136_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464848.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT        16478 a         7095 c         9092 g        12171 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta tggaaatgag
      541 aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt
      601 ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      661 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      721 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      781 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      841 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      901 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
      961 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     1021 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     1081 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     1141 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     1201 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     1261 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     1321 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     1381 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     1441 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     1501 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     1561 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     1621 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     1681 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     1741 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     1801 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     1861 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     1921 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     1981 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     2041 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     2101 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     2161 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     2221 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     2281 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     2341 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     2401 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     2461 aaatggggag atacagcagg taaaggactt tcttatgttg ctgaaaaagc agatattaaa
     2521 gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat
     2581 tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag tgatacagtt
     2641 gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa tgatagtgct
     2701 gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa tccatttaat
     2761 caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc aaaattctta
     2821 aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga aggcgcagaa
     2881 tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac agataaagat
     2941 ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct aaaatcagat
     3001 aaagagggtc attttgaaat cactggttta actgaaggag attactcact ggaagaaaca
     3061 aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt agacaaagat
     3121 tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc cgtaccaatg
     3181 actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct aggagcaggg
     3241 ggactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     3301 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     3361 gcagtagcag tctatttaat tccagttgtg gaaacaaatg tagcgaaatt tcaacaacag
     3421 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     3481 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     3541 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     3601 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     3661 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     3721 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     3781 ttacaaaaaa attatttaga ttatcttatg cctgaaaaag accaagatag gatcacgtta
     3841 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     3901 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     3961 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     4021 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     4081 ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     4141 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     4201 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     4261 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     4321 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     4381 tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc acgaatatct
     4441 tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata attgagtcaa
     4501 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     4561 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     4621 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     4681 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     4741 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     4801 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     4861 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     4921 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     4981 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     5041 aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     5101 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa tattagatgg
     5161 agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact ttagtgttgt
     5221 ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5281 cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat tcattgagaa
     5341 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5401 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa atcgttgccc
     5461 attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg actttttcta
     5521 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatggcaaaa
     5581 agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc atgcagtcaa
     5641 tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca aaagtagctt
     5701 tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca gctggtattg
     5761 atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag caacgtgtgt
     5821 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     5881 ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca gcatgtgcag
     5941 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6001 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6061 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6121 atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca caaccatcga
     6181 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6241 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6301 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6361 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6421 cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca ggtaatacag
     6481 tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt agggattata
     6541 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6601 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca gtagaaaact
     6661 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6721 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6781 tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat tacaagcaat
     6841 ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct gcgcgagtga
     6901 tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat gatagcaatt
     6961 caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt ttagatggac
     7021 gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7081 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7141 taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7201 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7261 tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt tgaagattgg
     7321 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7381 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7441 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7501 cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc tttccaataa
     7561 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7621 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7681 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7741 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7801 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7861 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
     7921 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     7981 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8041 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8101 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
     8161 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8221 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8281 attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8341 agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8401 aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8461 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8521 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8581 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8641 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8701 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8761 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8821 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     8881 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     8941 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9001 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9061 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9121 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9181 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9241 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9301 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9361 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggctaa
     9421 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9481 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9541 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9601 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9661 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9721 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9781 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9841 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
     9901 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
     9961 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10021 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10081 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10141 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10201 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10261 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta aataaataca
    10321 aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag gaggaatcac
    10381 agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag aacgccgtgg
    10441 tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg acctattacg
    10501 aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa aacttgtatt
    10561 cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc aattacaatg
    10621 aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc attacaaact
    10681 gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc agtggcttcc
    10741 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10801 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10861 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca agtaggagga
    10921 gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata ttcatgtttt
    10981 ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca acgaagaaag
    11041 tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttcggtcatt gaaacggaag
    11101 aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga
    11161 ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg
    11221 gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat ttaatccgcc
    11281 ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa
    11341 aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaagtga
    11401 gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca aagaaggagt
    11461 caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga
    11521 tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa
    11581 tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat gaagagtcaa
    11641 catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa gaagtagaaa
    11701 aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc
    11761 cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat
    11821 atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg
    11881 gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg
    11941 gacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta
    12001 atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag
    12061 aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac
    12121 gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag acgaatcaag
    12181 aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag
    12241 agttctaccg cggtttacgt acagataagc taaagattag caaagaatta aatgaacaac
    12301 tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa cagatccagc
    12361 aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat
    12421 tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa
    12481 gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc
    12541 aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc
    12601 gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag
    12661 tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg
    12721 gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata
    12781 tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    12841 aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc
    12901 agtcaacaac agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag
    12961 aaggatcaag cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg
    13021 tgaaataatg cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa
    13081 gaataggtat ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa
    13141 acatgacaaa cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga
    13201 gtatttagta acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt
    13261 acaaggaata aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc
    13321 tggtttttgg aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac
    13381 agtttgtgtg gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct
    13441 agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta
    13501 tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca
    13561 ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc
    13621 tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga
    13681 aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga
    13741 tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc
    13801 aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac
    13861 tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga
    13921 taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt
    13981 agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct
    14041 tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt
    14101 gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata
    14161 tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac
    14221 gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga
    14281 ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa
    14341 tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa
    14401 agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca
    14461 agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca
    14521 aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca
    14581 tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt
    14641 agtagagtct attcggatgc actataccga taaaccaatg gctattttta tgcttacacc
    14701 tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga
    14761 attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat
    14821 tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca tgacggtcac
    14881 agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac tttatgcgaa
    14941 gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat
    15001 gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga
    15061 acaagagaac agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga
    15121 ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag aaacgattgt
    15181 actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt
    15241 taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc
    15301 gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt
    15361 acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact
    15421 gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatctct
    15481 tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg
    15541 acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa tgattgggaa
    15601 acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg aattttttct
    15661 ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc caaaaccgta
    15721 acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt tagttggatt
    15781 tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac tttactagaa
    15841 aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg ggtaaatgca
    15901 ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat
    15961 ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat cttggttaat
    16021 ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat
    16081 atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa aacgggatca
    16141 cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact agcaactgac
    16201 ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc gaaaaatttt
    16261 aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat ggcaggtaat
    16321 gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca ataccgattg
    16381 gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg cttagcaaaa
    16441 tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat tttagctggt
    16501 gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac ttgcattatt
    16561 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacaggt
    16621 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16681 attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt gcatgcggca
    16741 ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat tgcgctattc
    16801 aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa aaacggcagt
    16861 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    16921 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaaaaat
    16981 ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
    17041 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
    17101 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17161 gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga cggcacgaga
    17221 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17281 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17341 gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    17401 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    17461 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    17521 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    17581 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    17641 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    17701 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    17761 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    17821 aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca tcaatcacct
    17881 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    17941 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    18001 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    18061 gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag gaacaatgca
    18121 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    18181 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tagtaacatg
    18241 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18301 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18361 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18421 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18481 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18541 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18601 tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc ctgtacagaa
    18661 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18721 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18781 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18841 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18901 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18961 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    19021 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    19081 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    19141 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    19201 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19261 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19321 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19381 ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt tttaaaacaa
    19441 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19501 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19561 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19621 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19681 aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga atatgctaga
    19741 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19801 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct caaagggatg
    19861 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19921 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19981 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    20041 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    20101 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    20161 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    20221 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20281 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20341 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20401 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20461 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20521 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca
    20581 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20641 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20701 gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag
    20761 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20821 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20881 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20941 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    21001 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    21061 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    21121 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    21181 tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    21241 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    21301 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    21361 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    21421 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    21481 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    21541 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    21601 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    21661 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    21721 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    21781 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    21841 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    21901 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    21961 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    22021 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    22081 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    22141 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    22201 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    22261 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    22321 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    22381 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    22441 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    22501 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    22561 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    22621 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    22681 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    22741 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    22801 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    22861 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    22921 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    22981 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    23041 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    23101 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    23161 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    23221 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    23281 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    23341 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    23401 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    23461 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    23521 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    23581 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    23641 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    23701 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    23761 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    23821 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    23881 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
    23941 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
    24001 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
    24061 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
    24121 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    24181 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
    24241 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    24301 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    24361 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
    24421 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    24481 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    24541 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
    24601 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
    24661 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
    24721 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
    24781 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
    24841 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    24901 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
    24961 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25021 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25081 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    25141 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    25201 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    25261 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    25321 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
    25381 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    25441 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    25501 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    25561 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    25621 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    25681 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
    25741 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    25801 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
    25861 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
    25921 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
    25981 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
    26041 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
    26101 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
    26161 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
    26221 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
    26281 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
    26341 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
    26401 agaaccacaa gatctgattt attacgtgca cgaagataag gttaaagatc aagtgaaaga
    26461 tgaagattat gaacaacacg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    26521 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
    26581 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    26641 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
    26701 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
    26761 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
    26821 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
    26881 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
    26941 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
    27001 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
    27061 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
    27121 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
    27181 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
    27241 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
    27301 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
    27361 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
    27421 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
    27481 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
    27541 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
    27601 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
    27661 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
    27721 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
    27781 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
    27841 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
    27901 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
    27961 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
    28021 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
    28081 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
    28141 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
    28201 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
    28261 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
    28321 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
    28381 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
    28441 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
    28501 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
    28561 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
    28621 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
    28681 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
    28741 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
    28801 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
    28861 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
    28921 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
    28981 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
    29041 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
    29101 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
    29161 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
    29221 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
    29281 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
    29341 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
    29401 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
    29461 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
    29521 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
    29581 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
    29641 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
    29701 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
    29761 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
    29821 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
    29881 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
    29941 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
    30001 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
    30061 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
    30121 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
    30181 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
    30241 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
    30301 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
    30361 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
    30421 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
    30481 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
    30541 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
    30601 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
    30661 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
    30721 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
    30781 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
    30841 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
    30901 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
    30961 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
    31021 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
    31081 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
    31141 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
    31201 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
    31261 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
    31321 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
    31381 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
    31441 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
    31501 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
    31561 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
    31621 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
    31681 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
    31741 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
    31801 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
    31861 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
    31921 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
    31981 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
    32041 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
    32101 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
    32161 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
    32221 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
    32281 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
    32341 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
    32401 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
    32461 tggaatggac acgtcggaga aagctattcc ttatattgca gcatggacga agaatggtca
    32521 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
    32581 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
    32641 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
    32701 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
    32761 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
    32821 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
    32881 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
    32941 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
    33001 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
    33061 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
    33121 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
    33181 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
    33241 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
    33301 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
    33361 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
    33421 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
    33481 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
    33541 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
    33601 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
    33661 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    33721 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    33781 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    33841 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    33901 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    33961 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    34021 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    34081 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    34141 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    34201 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    34261 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    34321 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    34381 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    34441 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    34501 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    34561 cacaagcgaa agcttatgcc gatagcttct ctaaagccac tcgaaaagac gggtattttg
    34621 aaatccaaga tcgattgttt cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag
    34681 tcgaattaga ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt
    34741 tgcctatttt ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca
    34801 aggtggtgaa acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc
    34861 gtgaagggga actgatcgct tggacgatca ttcaacaagt aggcgccgat caaggaaaaa
    34921 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccagc
    34981 agttacgaga cgcaaaagag acctatccaa aatttgaaga agcacaagca cgtcagatcg
    35041 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    35101 gcataccagg aagcttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc
    35161 aattgcagga aaagattaga aatttcaaga aagaacctta ttttgaagga aaatccgaag
    35221 taatcgctca aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc
    35281 aagaagcatt tgaagattat ttaaaagaaa agggggttca attgggaaaa cagccaggca
    35341 tgattcgcca agttgaaacc gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat
    35401 ccagccttca gtctaaaatg aatcagctca tgaaagctag tgccaaagac actttggaag
    35461 ccatgcaagg cttgtatgaa gggaaatatc taagctatcc acgaacagac accccttata
    35521 ttacggaagg ggagtatgcg tatttgttgg atcatttaga tgaatacaag cattttttaa
    35581 aggccgaatc aatcccaacc cctattcata cgccgaatag tcggtatgtg aataacaaga
    35641 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    35701 aacaactaag tccactgcag caggccattt atgaacaagt gttaaaaaca acggtggcca
    35761 tgtttgcgga aaaatatacc tatgaagaaa caacgatctt gacgcaagtc cagcagcttc
    35821 agttaaaggc aatcggaaaa gtaccgatag atctaggttg gaagaagtta tttggcaaag
    35881 agaacgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    35941 tggtgacggt agatctacaa gtccttgaaa aagaaaccaa gccaccacag ccttataccg
    36001 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    36061 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    36121 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    36181 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    36241 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    36301 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    36361 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    36421 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    36481 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    36541 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    36601 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    36661 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    36721 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    36781 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    36841 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    36901 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    36961 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    37021 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    37081 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    37141 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    37201 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    37261 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    37321 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    37381 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    37441 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    37501 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    37561 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    37621 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    37681 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    37741 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    37801 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    37861 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    37921 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    37981 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    38041 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    38101 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    38161 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    38221 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    38281 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    38341 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    38401 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    38461 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    38521 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    38581 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    38641 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    38701 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    38761 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    38821 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    38881 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    38941 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    39001 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    39061 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    39121 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    39181 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    39241 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    39301 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    39361 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    39421 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    39481 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    39541 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    39601 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    39661 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    39721 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    39781 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    39841 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    39901 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    39961 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    40021 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    40081 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    40141 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    40201 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    40261 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    40321 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    40381 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    40441 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    40501 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    40561 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    40621 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    40681 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    40741 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    40801 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    40861 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    40921 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    40981 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    41041 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    41101 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    41161 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    41221 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    41281 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    41341 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    41401 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    41461 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    41521 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    41581 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    41641 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    41701 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    41761 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    41821 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    41881 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    41941 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    42001 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    42061 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    42121 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    42181 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    42241 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    42301 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    42361 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    42421 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    42481 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    42541 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    42601 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    42661 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    42721 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    42781 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    42841 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    42901 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    42961 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    43021 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    43081 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    43141 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    43201 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    43261 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    43321 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    43381 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    43441 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    43501 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    43561 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    43621 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    43681 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    43741 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    43801 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    43861 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    43921 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    43981 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    44041 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    44101 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    44161 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    44221 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    44281 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    44341 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    44401 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    44461 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    44521 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    44581 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    44641 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    44701 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    44761 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    44821 tttctttttt tgcttt
//
LOCUS       DACDEA010000015        42973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDEA010000015 DACDEA010000000
VERSION     DACDEA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.015"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            598..1287
                     /locus_tag="IV136_000840"
     CDS             598..1287
                     /locus_tag="IV136_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464849.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IV136_000841"
     CDS             1274..2482
                     /locus_tag="IV136_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8464850.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IV136_000842"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IV136_000842"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8464851.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV136_000843"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV136_000843"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8464852.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IV136_000844"
     CDS             complement(6585..7280)
                     /locus_tag="IV136_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8464853.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV136_000845"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV136_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8464854.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IV136_000846"
     CDS             complement(8479..8670)
                     /locus_tag="IV136_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8464855.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IV136_000847"
     CDS             complement(8691..9581)
                     /locus_tag="IV136_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8464856.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IV136_000848"
     CDS             complement(9568..10335)
                     /locus_tag="IV136_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8464857.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV136_000849"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV136_000849"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8464858.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV136_000850"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV136_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8464859.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV136_000851"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV136_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8464860.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IV136_000852"
     CDS             14951..15289
                     /locus_tag="IV136_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464861.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IV136_000853"
     CDS             complement(15457..16215)
                     /locus_tag="IV136_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8464862.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IV136_000854"
     CDS             complement(16233..17051)
                     /locus_tag="IV136_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8464863.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV136_000855"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV136_000855"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8464864.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV136_000856"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV136_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8464865.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="IV136_000857"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="IV136_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8464866.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="IV136_000858"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="IV136_000858"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8464867.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="IV136_000859"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="IV136_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8464868.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="IV136_000860"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="IV136_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8464869.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="IV136_000861"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="IV136_000861"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8464870.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="IV136_000862"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="IV136_000862"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8464871.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="IV136_000863"
     CDS             27093..27392
                     /locus_tag="IV136_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8464872.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="IV136_000864"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="IV136_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8464873.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /gene="rpsR"
                     /locus_tag="IV136_000865"
     CDS             27980..28216
                     /gene="rpsR"
                     /locus_tag="IV136_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8464874.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="IV136_000866"
     CDS             28359..30332
                     /locus_tag="IV136_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8464875.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="IV136_000867"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="IV136_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8464876.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="IV136_000868"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="IV136_000868"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8464877.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="IV136_000869"
     CDS             33211..34503
                     /locus_tag="IV136_000869"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8464878.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35290..36153
                     /locus_tag="IV136_000870"
     CDS             35290..36153
                     /locus_tag="IV136_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8464879.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36282..37904)
                     /locus_tag="IV136_000871"
     CDS             complement(36282..37904)
                     /locus_tag="IV136_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8464880.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38153..38674
                     /locus_tag="IV136_000872"
     CDS             38153..38674
                     /locus_tag="IV136_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464881.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="IV136_000873"
     CDS             complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="IV136_000873"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8464882.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40755..41573
                     /locus_tag="IV136_000874"
     CDS             40755..41573
                     /locus_tag="IV136_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8464883.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41676..41873)
                     /locus_tag="IV136_000875"
     CDS             complement(41676..41873)
                     /locus_tag="IV136_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464884.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42042..42620
                     /locus_tag="IV136_000876"
     CDS             42042..42620
                     /locus_tag="IV136_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8464885.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13045 a         8246 c         8490 g        13192 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
    25441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
    25501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
    26881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    34621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    34681 tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
    34741 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
    34801 ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    34861 tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg gagctaaacg
    34921 gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
    34981 aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact caggtcaaaa
    35041 cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg gaaataagcc
    35101 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    35161 accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt
    35221 gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg
    35281 ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta
    35341 gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta
    35401 gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg
    35461 atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg
    35521 gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt
    35581 agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt
    35641 tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg
    35701 aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa
    35761 gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc
    35821 aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa
    35881 gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc
    35941 gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac
    36001 gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg
    36061 aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt
    36121 atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc
    36181 ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct
    36241 tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc
    36301 aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg
    36361 atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg
    36421 tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg
    36481 attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa
    36541 agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt
    36601 ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta
    36661 tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg
    36721 aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact
    36781 gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata
    36841 tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct
    36901 aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca
    36961 atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg
    37021 tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca
    37081 ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca
    37141 tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt
    37201 cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc
    37261 aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct
    37321 tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca
    37381 tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat
    37441 tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct
    37501 gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct
    37561 cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc
    37621 acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag
    37681 tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct
    37741 gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt
    37801 gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt
    37861 ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt
    37921 tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa
    37981 ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc
    38041 ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat
    38101 ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga
    38161 aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt
    38221 gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt
    38281 tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg
    38341 atataaagga aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt
    38401 cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat
    38461 tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata
    38521 ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt
    38581 taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt
    38641 tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg
    38701 gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata
    38761 tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata
    38821 cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa
    38881 ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc
    38941 ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt
    39001 gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg
    39061 aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc
    39121 gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc
    39181 ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt
    39241 gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa
    39301 tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat
    39361 agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat
    39421 agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg
    39481 attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga
    39541 tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc
    39601 agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat
    39661 cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa
    39721 ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat
    39781 cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc
    39841 aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg
    39901 aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc
    39961 aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt
    40021 ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc
    40081 aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata
    40141 ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg
    40201 attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata
    40261 gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg
    40321 tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc
    40381 gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg
    40441 acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt
    40501 gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac
    40561 cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac
    40621 ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt
    40681 ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa
    40741 gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg
    40801 ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta
    40861 aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg
    40921 ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata
    40981 ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag
    41041 ataaaacact gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc
    41101 atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt
    41161 tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat
    41221 tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg
    41281 tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag
    41341 cttctggaat cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg
    41401 agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg
    41461 aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg
    41521 gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa
    41581 aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt
    41641 aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta
    41701 tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc
    41761 gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca
    41821 attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac
    41881 cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct
    41941 ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct
    42001 ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg
    42061 aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg
    42121 aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata
    42181 ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg
    42241 tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc
    42301 tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga
    42361 cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg
    42421 tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat
    42481 cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag
    42541 tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca
    42601 tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa
    42661 aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat
    42721 caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat
    42781 caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca
    42841 ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt
    42901 acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa
    42961 agtgtatcga aaa
//
LOCUS       DACDEA010000016        42358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDEA010000016 DACDEA010000000
VERSION     DACDEA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.016"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..347
                     /gene="nrdH"
                     /locus_tag="IV136_000877"
     CDS             123..347
                     /gene="nrdH"
                     /locus_tag="IV136_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8464886.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            506..2665
                     /gene="nrdE"
                     /locus_tag="IV136_000878"
     CDS             506..2665
                     /gene="nrdE"
                     /locus_tag="IV136_000878"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8464887.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2715..3680
                     /gene="nrdF"
                     /locus_tag="IV136_000879"
     CDS             2715..3680
                     /gene="nrdF"
                     /locus_tag="IV136_000879"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8464888.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3769..4908)
                     /locus_tag="IV136_000880"
     CDS             complement(3769..4908)
                     /locus_tag="IV136_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8464889.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5217..5930)
                     /locus_tag="IV136_000881"
     CDS             complement(5217..5930)
                     /locus_tag="IV136_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8464890.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6184..6885)
                     /gene="nagB"
                     /locus_tag="IV136_000882"
     CDS             complement(6184..6885)
                     /gene="nagB"
                     /locus_tag="IV136_000882"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8464891.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7119..8651
                     /locus_tag="IV136_000883"
     CDS             7119..8651
                     /locus_tag="IV136_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8464892.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8842..10044)
                     /locus_tag="IV136_000884"
     CDS             complement(8842..10044)
                     /locus_tag="IV136_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8464893.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10126..10815)
                     /locus_tag="IV136_000885"
     CDS             complement(10126..10815)
                     /locus_tag="IV136_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8464894.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10921..11289)
                     /gene="rplL"
                     /locus_tag="IV136_000886"
     CDS             complement(10921..11289)
                     /gene="rplL"
                     /locus_tag="IV136_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8464895.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11342..11845)
                     /locus_tag="IV136_000887"
     CDS             complement(11342..11845)
                     /locus_tag="IV136_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8464896.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12255..12944)
                     /gene="rplA"
                     /locus_tag="IV136_000888"
     CDS             complement(12255..12944)
                     /gene="rplA"
                     /locus_tag="IV136_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8464897.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(13048..13470)
                     /gene="rplK"
                     /locus_tag="IV136_000889"
     CDS             complement(13048..13470)
                     /gene="rplK"
                     /locus_tag="IV136_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8464898.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13813..14481
                     /gene="sdaAB"
                     /locus_tag="IV136_000890"
     CDS             13813..14481
                     /gene="sdaAB"
                     /locus_tag="IV136_000890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8464899.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14494..15366
                     /gene="sdaAA"
                     /locus_tag="IV136_000891"
     CDS             14494..15366
                     /gene="sdaAA"
                     /locus_tag="IV136_000891"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8464900.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15457..16476)
                     /locus_tag="IV136_000892"
     CDS             complement(15457..16476)
                     /locus_tag="IV136_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8464901.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16649..17536)
                     /locus_tag="IV136_000893"
     CDS             complement(16649..17536)
                     /locus_tag="IV136_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8464902.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17613..18158)
                     /gene="nusG"
                     /locus_tag="IV136_000894"
     CDS             complement(17613..18158)
                     /gene="nusG"
                     /locus_tag="IV136_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8464903.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18259..18429)
                     /gene="secE"
                     /locus_tag="IV136_000895"
     CDS             complement(18259..18429)
                     /gene="secE"
                     /locus_tag="IV136_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8464904.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18453..18605)
                     /gene="rpmG"
                     /locus_tag="IV136_000896"
     CDS             complement(18453..18605)
                     /gene="rpmG"
                     /locus_tag="IV136_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8464905.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18767..19411
                     /locus_tag="IV136_000897"
     CDS             18767..19411
                     /locus_tag="IV136_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8464906.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19467..19970)
                     /locus_tag="IV136_000898"
     CDS             complement(19467..19970)
                     /locus_tag="IV136_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8464907.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19983..20029)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20567..21532
                     /locus_tag="IV136_000899"
     CDS             20567..21532
                     /locus_tag="IV136_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8464908.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21631..22803)
                     /locus_tag="IV136_000900"
     CDS             complement(21631..22803)
                     /locus_tag="IV136_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464909.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22790..23935)
                     /locus_tag="IV136_000901"
     CDS             complement(22790..23935)
                     /locus_tag="IV136_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464910.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(23949..24873)
                     /locus_tag="IV136_000902"
                     /pseudo
     CDS             complement(23949..24873)
                     /locus_tag="IV136_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            24990..26069
                     /locus_tag="IV136_000903"
     CDS             24990..26069
                     /locus_tag="IV136_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8464911.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            26101..26694
                     /locus_tag="IV136_000904"
     CDS             26101..26694
                     /locus_tag="IV136_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8464912.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(26804..27031)
                     /locus_tag="IV136_000905"
     CDS             complement(26804..27031)
                     /locus_tag="IV136_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8464913.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27134..29722)
                     /locus_tag="IV136_000906"
     CDS             complement(27134..29722)
                     /locus_tag="IV136_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8464914.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29742..30401)
                     /locus_tag="IV136_000907"
     CDS             complement(29742..30401)
                     /locus_tag="IV136_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8464915.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30450..30953)
                     /gene="trmL"
                     /locus_tag="IV136_000908"
     CDS             complement(30450..30953)
                     /gene="trmL"
                     /locus_tag="IV136_000908"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8464916.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31174..32040)
                     /locus_tag="IV136_000909"
     CDS             complement(31174..32040)
                     /locus_tag="IV136_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8464917.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32149..32790
                     /locus_tag="IV136_000910"
     CDS             32149..32790
                     /locus_tag="IV136_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8464918.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32852..34213)
                     /gene="mgtE"
                     /locus_tag="IV136_000911"
     CDS             complement(32852..34213)
                     /gene="mgtE"
                     /locus_tag="IV136_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8464919.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34231..35130)
                     /locus_tag="IV136_000912"
     CDS             complement(34231..35130)
                     /locus_tag="IV136_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8464920.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35132..35929)
                     /locus_tag="IV136_000913"
     CDS             complement(35132..35929)
                     /locus_tag="IV136_000913"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8464921.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(35907..36611)
                     /locus_tag="IV136_000914"
     CDS             complement(35907..36611)
                     /locus_tag="IV136_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8464922.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36742..37317
                     /locus_tag="IV136_000915"
     CDS             36742..37317
                     /locus_tag="IV136_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8464923.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            37435..38112
                     /locus_tag="IV136_000916"
     CDS             37435..38112
                     /locus_tag="IV136_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8464924.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38191..39999)
                     /gene="pepF"
                     /locus_tag="IV136_000917"
     CDS             complement(38191..39999)
                     /gene="pepF"
                     /locus_tag="IV136_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8464925.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40175..41173)
                     /locus_tag="IV136_000918"
     CDS             complement(40175..41173)
                     /locus_tag="IV136_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8464926.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41399..42052)
                     /locus_tag="IV136_000919"
     CDS             complement(41399..42052)
                     /locus_tag="IV136_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8464927.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12806 a         8812 c         7481 g        13259 t
ORIGIN      
        1 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
       61 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
      121 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
      181 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
      241 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
      301 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
      361 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
      421 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
      481 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
      541 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
      601 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc
      661 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
      721 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
      781 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
      841 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
      901 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
      961 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     1021 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     1081 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     1141 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     1201 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     1261 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     1321 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     1381 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     1441 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     1501 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     1561 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     1621 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     1681 tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg
     1741 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     1801 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     1861 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     1921 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     1981 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     2041 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     2101 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     2161 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     2221 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     2281 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     2341 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     2401 tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg
     2461 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
     2521 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
     2581 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
     2641 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
     2701 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
     2761 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
     2821 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
     2881 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
     2941 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
     3001 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
     3061 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
     3121 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
     3181 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
     3241 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
     3301 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
     3361 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
     3421 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
     3481 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
     3541 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
     3601 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
     3661 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
     3721 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
     3781 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
     3841 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
     3901 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
     3961 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
     4021 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
     4081 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
     4141 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
     4201 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
     4261 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
     4321 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
     4381 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
     4441 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
     4501 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
     4561 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
     4621 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
     4681 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
     4741 ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat
     4801 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
     4861 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
     4921 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
     4981 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
     5041 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
     5101 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
     5161 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
     5221 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
     5281 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
     5341 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
     5401 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
     5461 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
     5521 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
     5581 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
     5641 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
     5701 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
     5761 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
     5821 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
     5881 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
     5941 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
     6001 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
     6061 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
     6121 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
     6181 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
     6241 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
     6301 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
     6361 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
     6421 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
     6481 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
     6541 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
     6601 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
     6661 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
     6721 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
     6781 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
     6841 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
     6901 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
     6961 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
     7021 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
     7081 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
     7141 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
     7201 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
     7261 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
     7321 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
     7381 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
     7441 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
     7501 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
     7561 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
     7621 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
     7681 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
     7741 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
     7801 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
     7861 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
     7921 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
     7981 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
     8041 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
     8101 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
     8161 atattgccat tcgaggcttc aacgggattg ggaaatctac tttattaaaa acactgatcg
     8221 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
     8281 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
     8341 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
     8401 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
     8461 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
     8521 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
     8581 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
     8641 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
     8701 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
     8761 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
     8821 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
     8881 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
     8941 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
     9001 gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta gcccttgtac
     9061 tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa
     9121 aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat
     9181 aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat
     9241 aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc
     9301 ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt
     9361 tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa
     9421 atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga
     9481 acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa
     9541 atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa
     9601 aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc
     9661 cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa
     9721 aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac
     9781 ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg
     9841 aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac
     9901 tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata
     9961 catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa
    10021 aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta
    10081 tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg
    10141 ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt
    10201 aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc
    10261 acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg
    10321 tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat
    10381 catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca
    10441 atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt
    10501 gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct
    10561 caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc
    10621 tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag
    10681 ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata
    10741 caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat
    10801 tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac
    10861 ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa
    10921 ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc
    10981 ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc
    11041 tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc
    11101 agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc
    11161 agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac
    11221 taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt
    11281 caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca
    11341 attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc
    11401 ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt
    11461 ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga
    11521 tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat
    11581 cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc
    11641 cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag
    11701 catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa
    11761 cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt
    11821 tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag
    11881 ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca
    11941 tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc
    12001 gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa
    12061 aaaactctat gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg
    12121 gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca
    12181 actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac
    12241 attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt
    12301 gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat
    12361 acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg
    12421 attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact
    12481 tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag
    12541 ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca
    12601 acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct
    12661 acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa
    12721 acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct
    12781 gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca
    12841 atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca
    12901 acttttttta atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga
    12961 ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg
    13021 agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt
    13081 tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa
    13141 ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc
    13201 gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt
    13261 tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc
    13321 tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc
    13381 ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa
    13441 tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg
    13501 ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa
    13561 ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat
    13621 ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa
    13681 caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat
    13741 gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat
    13801 gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg
    13861 atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt
    13921 agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa
    13981 acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc
    14041 gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg
    14101 tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa
    14161 ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct
    14221 gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac
    14281 caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat
    14341 atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa
    14401 gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac
    14461 agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta
    14521 gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg
    14581 agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa
    14641 caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat
    14701 gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta
    14761 aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt
    14821 gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat
    14881 cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg
    14941 ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt
    15001 ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat
    15061 caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc
    15121 gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct
    15181 tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc
    15241 gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa
    15301 ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg
    15361 gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac
    15421 gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt
    15481 tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag
    15541 gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg
    15601 tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg
    15661 acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg
    15721 caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca
    15781 gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt
    15841 tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc
    15901 ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact
    15961 ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac
    16021 gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc
    16081 tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa
    16141 ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc
    16201 ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc
    16261 ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa
    16321 ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg
    16381 catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga
    16441 accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag
    16501 ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt
    16561 agatccttct ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc
    16621 aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct
    16681 gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt
    16741 tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta
    16801 acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc
    16861 gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga
    16921 cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat
    16981 taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat
    17041 acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt
    17101 tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat
    17161 tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc
    17221 cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt
    17281 ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc
    17341 cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt
    17401 ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac
    17461 ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt
    17521 tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc
    17581 caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa
    17641 tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt
    17701 tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc
    17761 acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc
    17821 aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa
    17881 ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg
    17941 atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca
    18001 tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt
    18061 ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat
    18121 gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt
    18181 aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag
    18241 tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc
    18301 cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt
    18361 acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag
    18421 aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat
    18481 tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg
    18541 actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt
    18601 gccatttact tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta
    18661 actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat
    18721 gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac
    18781 acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc
    18841 attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa
    18901 tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga
    18961 tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac
    19021 ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag
    19081 tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc
    19141 ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga
    19201 tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct
    19261 tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga
    19321 agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga
    19381 aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat
    19441 ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct
    19501 aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg
    19561 ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc
    19621 atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg
    19681 ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca
    19741 tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa
    19801 agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc
    19861 ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg
    19921 ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct
    19981 cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac
    20041 aataattata acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa
    20101 aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa
    20161 ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg
    20221 tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg
    20281 tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta
    20341 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
    20401 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
    20461 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
    20521 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
    20581 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
    20641 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
    20701 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
    20761 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
    20821 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
    20881 agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
    20941 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
    21001 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
    21061 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
    21121 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
    21181 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
    21241 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
    21301 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
    21361 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
    21421 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
    21481 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
    21541 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
    21601 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
    21661 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
    21721 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
    21781 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
    21841 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
    21901 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
    21961 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
    22021 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
    22081 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
    22141 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac
    22201 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
    22261 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
    22321 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
    22381 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
    22441 atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa
    22501 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
    22561 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
    22621 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
    22681 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
    22741 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
    22801 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
    22861 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
    22921 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
    22981 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
    23041 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
    23101 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
    23161 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
    23221 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
    23281 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
    23341 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
    23401 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
    23461 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
    23521 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
    23581 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
    23641 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
    23701 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
    23761 ccgctttgat tacaattcca gtattaatcg tttcgagatt gccaccttga tcaatctcgc
    23821 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
    23881 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
    23941 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
    24001 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
    24061 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
    24121 attccatcag aagctattgt tcgctgacaa cacgttttct gggagtaatg ggatgtcgat
    24181 cgtcaaacga tccgccgtcc gtaccatatc ttttagagat tctttggttc ctttagcaat
    24241 cacctgtcct tgatccatga tcacgatctg gtcgcaaaga atttctactt cttccatgta
    24301 atgtgttgta tagacaatgg ttgctccttg tcgattcagc tccttgatac tttctaaaat
    24361 tttattccga ctttgggggt ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg
    24421 cttgtgggca atcccacatg cgatgttcaa ccgacgtttc aagccaccac taagttctcc
    24481 tggacgaaac gaaacatatt tttctaatcc cactaattga atcacctctt caatcaaggc
    24541 tttccttttc tctttttcgg aaacatataa accgcaaaaa taatcgatat tttcttgcac
    24601 acttagttcg tcaaagaccg caacgtcttg aggaacgacc ccaatcattg ccttgacagc
    24661 ataattttca ggagtcattg ttgtcccgaa caatttaatg gttcctttgt cataatttaa
    24721 caaagaaagc atacagttga tggcagtcga tttcccactg ccgtttggtc ctaacagacc
    24781 taagacttct ccttcttgta cttgcagatc aaaatgattc aatgctacaa aatctccata
    24841 gcgtttgact aatttatcaa tttcgataat catcatgaaa aactccttcc aaatttcttg
    24901 ttgatctgac tataataaag tgcaagaaaa gaaactagtg acgattggct aaaccacaca
    24961 tgacatttgt cacaatgaag gagtctgcta tgcataaatc tcggctactc aacgaaacta
    25021 gctttctact ggttatctca ttgcttttgc tggtaagtca aggattcgtt gctatgcaag
    25081 ttatctattt attgatcgcc gttttattca ctggtgtcag ttatttactg cctacaaagc
    25141 caaattggat tttccaagtt attctatttc ttttatgtgc ctttttacct gatcttgtcc
    25201 tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga aagccacctt gattacttgt
    25261 ttttgttagg ttttttgttg atttcgacgg ttggtaatca tccccccatc aaggtcgcct
    25321 ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg
    25381 atcttgagaa aaagtacatt gtctccaaag atactcattg ggaaaaacaa caagagttat
    25441 cggaacaaaa taaagcattg gccctgtcac aagaaacaca attagccttg caaattgtgg
    25501 aagaacggaa ccgaattgcc cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg
    25561 cgattttaca aattggtgcg cttgaaatga tcaatcaaga accaaaacta aaagaaccat
    25621 tagaaaattt aagtgccacc gttcacactg ggatggatcg gattcgtcaa agtgtccatg
    25681 acctccatga gacatcgatc tcttttcaac actccttaga gtttctgatc gctgattttc
    25741 cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact
    25801 gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat caaacatagt catgccaaaa
    25861 ccgttgtttt acattttcga acgttgccgg ccttttatcg cttgcaaatc acgaatgatg
    25921 gcaccagccc acaaaaaaat tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg
    25981 ttcaagaaca aaagggacaa gtccatattt tccaagaaaa ccagcaattt cagttaacga
    26041 tcattttacc aagggaggga caccaatgat aacagtagtc attattgaga tgatccattt
    26101 gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt
    26161 ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc atcgtcccga tgttttgctg
    26221 acggacatcc gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat tttggctaaa
    26281 tttcccaaag ccatcctttt gcttttgacg acattcaaag atgaagaata catccgcgaa
    26341 gcattttcca tcggtgtcaa agggtattta atcaaacaga acttgcaagc gattattcct
    26401 tctgtcaaat ccgcttataa tggacaggcc gtatttggta atgaaatcat cgagactttc
    26461 actcaaatga tgaagaataa accgacgatt gatagtcatg cttatgcttc ttttagtgaa
    26521 cgtgagcttg caatcatcaa agaagtagct gccgggaaaa acaataaaga aattgcagat
    26581 gccttgtact tgagtgacgg cactgttcgc aattacatca gccaattact agaaaagcta
    26641 gatttacggg atcgcacgca attggctatc tatttttatc agcatttgca ataagtctta
    26701 tctaacacaa aaaaacctga atgatgctcc ttcgcatcat tcaggttttt ttcacatctt
    26761 ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga ttcgcagggc
    26821 tgccaggatt cgttggattg ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg
    26881 ttttttctag ttttgattct tcacgatcgt ctcgttctac atcaggactt tcattcaatt
    26941 tgaattcttt ttccatctca tctaactttt taaagtcatt gccagattct tttgctaatt
    27001 tcgctaattt atcttcggtt tctttagcca taaagtaacg cccccctttt gatttcagct
    27061 tagcatatca cactgaaaaa acaaaagaaa acgctaacca acgatcattt ttagctattt
    27121 ctcatcttta aaatcaagca ttttttgccg gtataaaatc gtagggagaa ctgttttcca
    27181 taccaatcat cggatctact aactggttat cgatcacttc tgctgtaaga acgaaatctt
    27241 tagaaacagc aggttctacc tcttctttct tttcttctcc aacaaatgaa gaaaacagat
    27301 ctggttccac tgtttccatt acttggtttt tcgtttcatt cgtcccactg tcatcttcta
    27361 caggtagctt ttcttttaca acccgtggct cttttgtata gttcccatct tcaaatggat
    27421 catttccgac caaagaatct tcataagctg ctagtttggt ccggagagtg atattcattt
    27481 tatcattatc catgatcctt gatttcaacg tagtcatccg gcttgctgat tcattcgcta
    27541 cacaagtgac ataggcttgc tcttccccta acaaaatcaa cagttcctta ctgcgagtca
    27601 ctgccgtgta gagcaaatta cgctgaagca tccgctgata ttgatggacc atgggcaaaa
    27661 taaccatctt gaattcactg ccttgtgatt tatggattga gcaacaataa gaaagagtga
    27721 ttttattcca ttcgtttctt ttataagtga cctcatttgc atcaaactgg ataactaatt
    27781 catccacttt atcttcagaa tcttttgcca agatgatacc cgtgatttct cccatatctc
    27841 cattgaatac attcaattcg ggagtattga ctaattggag gactttgtct cctatccgat
    27901 aaacggtgtc gttccaatgt acttcctttt ttccaggttt ccctggattg aatatctcct
    27961 gcatcatttt atttaacgca tcgatccctg caggtccccg atacattggt gctaatactt
    28021 ggatatcttg tgcacggaat cccttatttt tcgctttttc cacaatttta gagatcaagg
    28081 gttcaatttg ataagcatca cttcggaaat aggaacgatc tttttgattc tttgtgaaat
    28141 cttttggcag acgtccttct ttgatctcat gggcaagagg aataatacta gaaccatcac
    28201 cttgccgata aatctgattg agttccattt tgggaatttg ttcgatttcc aaaagatcat
    28261 ggagtacttg accaggaccg acagagggaa gctgatcttt gtctcctact aagataactt
    28321 gcatattcgt cggaatcgct ttgaataatg tgttagccag ccatgtatcg accatagaca
    28381 tctcatcaac aatcaaaagt ccgccttcta attctttcgt actgactgaa ggtgtttttt
    28441 cccgtccatt cagccccaga agacgatgga tcgtactggc aggcagacct gtcgtttcgt
    28501 tcattcgttt agctgctcgg ccagttggtg ctgcaagtag aataggaaaa atttcttggg
    28561 tatattttga aggatccaat tccagtccgt tcaactctgc aaaaagattg acgattccgt
    28621 tgatcacagt agttttcccc gtacctggtc ctcctgttaa aataaataga ggggaacgga
    28681 ttgcttcagc gatagcttgt tcttgcgaag gtccatactg tatgccgaat attttttcga
    28741 tacgccgaat attcttctgc acttcttttt caggataatg gatttctttt ttacgactga
    28801 gaaggcgctg gatcgaatta ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa
    28861 gattagtctc ttcttgttgt atctttcctt cttcaacaag ttggataatt acagtagcaa
    28921 cctgatcagg ctggatttca attgggcgac ttgtttctaa aatacggatc gtttgctgaa
    28981 gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac tgcatgctga aaaatctgat
    29041 gcaagatagc tgctcgaatt cttctcggtg aatctgctgc aataccgatt tgttcggcta
    29101 gattatctgc acgtttgaac ccgattcctt ctatgtcttc gactagctgg tacggatttt
    29161 cttcgatgat atccaaagac tcgttcttat aggcctggta gatactgaaa gctaactgac
    29221 taccgaaccc gtaacgattc agtcccatga tcacttgatc catcccgtgg tttagccgaa
    29281 tcgtctcagc aagcatttct ctttttggct tcgtcaaacc ggagatttgt tttaataccg
    29341 aaggatcagc aatgattttt tcgatagcat cttctcccag caattctaca attttttctg
    29401 ctgttttctt cccgattcct gggaattttt cgctggaaag atacgctacg atcccatttg
    29461 cagttgttgg cttttcttgc tcatatcttt cgagtttaag ctgccttccg tatttaggat
    29521 gatccactaa ttctccataa aaacgataag tctcattttc ttgaatatcg ccgaaacttc
    29581 cggtgactac aatctctttt tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa
    29641 gcactttgta aaaattacta ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat
    29701 aaggcatttc ttctggctgc atcggaagcc gcctccttct tctatagtaa aggttctggt
    29761 gctgtttcag ttcctaaaaa atctgtttgg ttccatactt tcagttcata tggcaataat
    29821 ctgcaaggct cttcagtctc taaaatcgtc aaactactat tgaacaatcc acccatttca
    29881 cgcaactcac ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa agaagctcca
    29941 tgtcctacga ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc tacaacagtt
    30001 tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga
    30061 tctaaatgat tacgcaaatt cggcagatta tccggatatt tctggtacat ttcatcgatc
    30121 gactgacctt ctaaagtacc cagccctagt tctcttagct tatctgtata aaaaatctct
    30181 accggacgag caagctcttt gttgatacct tctgctgtat cacgtgctcg ctttgctgta
    30241 ctggaataaa ttgctttaaa aggaacatat tttacctttt tcccaaatgc tttgatttct
    30301 tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg tctttcctga
    30361 ttccattgtg tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa actcctgtct
    30421 ttcttttatt ttactctata ataaactgtc taatccagct tgtcattttc atagtgatgc
    30481 gtcaattcta aattagggaa tccttgctgg cgaagtgctt catacactat cattgcagct
    30541 gtattcgata aattcaatga gcggacatgc tcatcattca tagggatgcg taaacacttt
    30601 tcttcgtttt cacgcataaa tgtttctggc aaaccggttg tttcttttcc aaataaaaag
    30661 aagtgctctt ctccatcagc aaaattttcg tcactatata ctttatgggc aaattttgag
    30721 atcaaatgta gtttacgatt acctaaaaat gttaaaaaag cttctaaatt actgtggtaa
    30781 gtaatattta catcattcca ataatccaac cctgcccgtt ttagatgttt gtcatcagtt
    30841 gaaaaaccta gtggctcgat taaatgaagc ggagtattcg tggctgcaca cgtacgggca
    30901 atatttccag tgtttgcagg gattaatggt tcaaataaaa cgatatgatt catcaggatc
    30961 attcctctca tatttatgtt cgtttctatt ttctgacaat ttgatttttg cataaaaaaa
    31021 aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct cttctcacta
    31081 acttttatca gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata
    31141 acaccctcaa cttttttttg caacctctac tcattaaaaa ctgctagaaa aacgttttct
    31201 cttagttcct tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa
    31261 gtctgctttg attcgctctt ctacctgcca ttctaaaggt gacatggcca aaagatcttt
    31321 ttgattttcc tctggtatct caaattcata ggttacacgc tgacgttcag ttaatttcat
    31381 tgcttcagca aacttggcta ctacacgttc attggaataa ctctgttttt caggttgatt
    31441 cggataaaaa gcctgacgta gttcctttaa ataattttct tgtggcacca ccttcaacac
    31501 ttctccatct tctgagagta cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt
    31561 taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag ctacacacca
    31621 aaatgcttcg gcagattgtt ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc
    31681 aacagcattt tgaatctttt tcaattttga aagttcattc aagaagctac cttctccgca
    31741 gccaacatct aatagcgaga cttcttcagg taaatgctgc gcaatagtct caataacggg
    31801 ctgatacatg ccagattgga tcattctacg ccttggaacg aacattcctt gatcgtattc
    31861 tgttttaacc gcatgggata aaaaataaag tgttcctttt ttggaaaggt caaaacggtg
    31921 attatttttg catatcaagc cattatcaga cttttttact tcttcttggc aaaccggaca
    31981 gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat
    32041 agttccccct catttctgtt ttatcttacc gttagatgct cactttggca actgctaaaa
    32101 atatgataca atgcctagaa gaaagtgaaa gataaaggag aagattagat gatcaaagaa
    32161 ttttgcgctg aaaactacac atctattcct ctagcaattg ctagaggagc cgatcggatc
    32221 gaattgtgtg acaacttagc ggtcggtggc acaaccccga gtactggtgt gatcgaagaa
    32281 acgttaagtt atgctgggga aaaagatatc cccgtaatga cgattattcg tcctagaggg
    32341 ggcgacttcg tctataacga tatcgaattg aaaatcatgc atactgattt gattgaagca
    32401 aagaaattag ggacggatgg cgtagtattt ggttgtctta ctccttcagg ctggctagac
    32461 gaagaggcct tagaactatt gatcgaaaca gcagaagggt tgcagatcac cttccatatg
    32521 gcttttgaca gtattcctga agatcggcaa ttcgaagcaa tcgattggct ggtagaacat
    32581 ggtgttgacc gtatcttgac tcatggcggt cctgctgaca aaactattgt agaaaatttc
    32641 ccacggctta aagaattgat tgcctataca gatggccgct taatcatttt gcctggtggt
    32701 ggtgtcactg cagagaatgc agcacatgta gctaaagaat tgaacgtaag cgaagtacac
    32761 ggaaccaaaa tcgtggatct tcagttataa tcttataaac aaaaaaacat ttgcaacagg
    32821 cataatgctt attgcaaatg tttttgtttt tttacataaa atagcccata aataaagaag
    32881 caatattgaa ataaatcagt acactcgtca aatcgctcaa agtcgtgata aagggaccgc
    32941 ttgctactgc tggatcaaat cctagacgat ccattagcat cgggattaaa ctacctgcaa
    33001 gattcgccac tgtgattgca aatagcatag ccatccctat gacaaacccc aagataaaat
    33061 tatgtttcca aaaacctacg attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa
    33121 cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa
    33181 tcgcaagtcg acgaacagcc acagctagcg actgggttcc agcattaccg gcagttcctg
    33241 taatcagcga aatgaagact gctaaaatac tcgcttcact taccaagtct tcatagtgac
    33301 tgattaaaga agcggtcgac atacctaaaa acaacagagt gatcagccaa ggcaaacgtc
    33361 gagacgctgc ctttactgga ttttcattaa tttcttccac attgaccccg gcaagtcctg
    33421 aataatcgct agcagcttca tcatcaataa cgtcgatgat atcatcgact gtgacgattc
    33481 ctaacaaatg atcatcataa tcggtaacag gaagagctaa gaagtcataa tctctaaatg
    33541 tttgtgccac atcttcttgg tcgtcgccta catgaactga aagcactcgt tcgctcatca
    33601 gatcagaaat cattgcgtcg tcatcattca cgattaggtc gcgcagagaa ataaccccta
    33661 ctagctgacc ttcttgatta acgacataga tgtaatagat tgtttcagct acatctgctt
    33721 cgttcttcag cacgtacatc gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt
    33781 cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc ttgatttcac
    33841 tggcatcgtc tgccgacatc aaacttaaat atttagcaat ttgcttttta tccagcgtat
    33901 taagcaagtc aacagcatta tctgtataca tctcagaaag catatcagct gcatagctag
    33961 gacgcatttc atctaggtat tctttcatat gttcgttgtc ttcttcaatc acatcgaaca
    34021 tatccgctaa ttctttaggc gacagatagg aatagatcag ctgcctgttc tcttctgtaa
    34081 gagattggta gaattgcccc tgctcataga tatgcatctc taaaaaactg tttctgaatg
    34141 cctgaatatt attttccctt aaatcttgag tcaactgggc aaatctttcc tctaattctt
    34201 gattttcatc caaaccaatc tcacctcgcc ttattcttca aaccgtctca caactgcctc
    34261 catgtctgcg gctaaaggct gcttgaactc cagcaactct tctgtaaatg gatggtaaaa
    34321 acgcagatca taacaatgaa gggcttgtct ttttattccc agatccattc gcccgccata
    34381 catatcatca ccgagcaggg cacagccaat tgcggaaaaa tgaactctta tttgatgtgt
    34441 ccttcctgta tgcagctgga tatcaactaa cgcgatttct gttagtcgct tctctatcca
    34501 gtactcagtc tgtgccgaac gtccttcatc agagaccatt cgcttcaata gcgaagaata
    34561 atctcgggcg atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa gccttccact
    34621 gactaaggcc tgatattttt tcacaaactt cttttcacga agttgcatat ctaattttgc
    34681 gtgcgcaaat ccatgctttg caaaaagcat caaaccagat gtatcccgat ctagtcgcgt
    34741 caccacatgg attacttggt tttcgtaacc ttgtttttta taataagcct ttacacgatt
    34801 agccattgtc ccatttggat ggtattgggc aggaatagac gaaacgcctg ctggtttatt
    34861 tactaccaga acgtgttcat cttcatacac gatatcgatc ggtgtttcat ccaaaagtac
    34921 ggtttcatgt tctccttcag caggaatcac aatggttacc ttatcatcct tagctagaga
    34981 aaacaagaca ttttctactt gatcattaac tttgatttga ccgccttgaa attttatttt
    35041 cgctaacaga ccctttgaga tccctttttc ttttaaaaaa tattttactt gctgagaatg
    35101 atctttctca taatcccatg taaactgcat atcaatcctc actaataaag gcatctttca
    35161 cacgatgcca aaaatgcata tgtcgataag aagcaaaatg gatccgttct tctgctattc
    35221 gataataaat tgagcggata tttgcttttg cgacattcaa ttgatcaacc gttacaagat
    35281 aatcttctgt attttccaat tttatttcta gccattcatc atgggcaatc acgatcggag
    35341 aacccaaagt acgaaacact cgattattca gtgaagcaat ctctgccaac tgaaaggcat
    35401 tgatactagg atggatcaca gcaccgccga cacttttatt atatgctgtc gaaccagtag
    35461 gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa cagctcgttt ttgatgtata
    35521 catcggctac catcgtacga ttggcccgtt taatcgttga ttcattcagt gctaaaaaat
    35581 gctggtccgg tgtttcatcc aaataactga tccgaacatc cagcaaggga taactaacac
    35641 tttgttcccg gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa tcccgccaat
    35701 ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa acgcacatca ttcagacaat
    35761 gactgtagtg atggaacgcc gacaataatg tgccgtctcc gccgactgag atgaccaatt
    35821 ctggctcatg atcatctcgt tgaatccctg cttgttctaa aagtgcgttc aaacgttctg
    35881 tcacttctat agtctttgct tctttattat gaacgatcgc tactttcatc ccctgcctcc
    35941 tctctttcgc tttctttctt caaaagcgtt cgatagtaga catcatctga ttttcctctt
    36001 ccatgggaga ataaccgctg tgcttcttgg atttcttcac ggatactgga catctcttca
    36061 tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa
    36121 acgccttgat atttataatt cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga
    36181 gtccgaatct ggatttccgc caaaattttc ttctcaccgc tgatcagctg tacaggatac
    36241 tcgatcacca gatgataaga acggtagcca cttgcttttt tatttgtgat gtaatctcgt
    36301 tcttggagaa ctttcatgtc ttttctttta cggatgatct caacaacttg atggatatct
    36361 tctacaaact gacacatgat ccttaacccg gcaatatcag acatttcttc ttctaaacgt
    36421 cccattggaa tatgccgtaa ttgtgatttt gttacaatac tatcgactgg cttgactctc
    36481 ccagtgacaa attcaatcgg gacatgtcga ttttgttcac ggaactgttt gcggatcccc
    36541 cgcagtttca ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa
    36601 tctcgttcca tagctgatac ctctacttca ttaacattca ttgtttattc taccatatat
    36661 gcaaagtttt ttcgtactct tgatttatgc attgaccaaa aaaagaaaat taggcaaaat
    36721 agacaaaaag gagtcgatca aatgacacaa atgcttgaaa tcgaatataa atctatgctg
    36781 acaaagcaag agtacgagat attcatcgct cactatcaat taacagataa agatttccgt
    36841 gtacagacta atatttattt cgataccgtt gatgaacagc tgaagaaaca aaattgtggc
    36901 ctacggatcc gtttcgttgg tcatcaagct gaatatacat taaaaacacc tgctgaaaaa
    36961 gggcgtctcg aaacaacgga tacctttata acagaagaag cagaagcatt tatcttcgaa
    37021 aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa tatcgatcct
    37081 gcttcattaa tccaaaccgg aaagcttaca acaaaacggg ctgaattccc tattgaagaa
    37141 ggacttttag ctattgatga aagctgggga gacaatctac acgattttga actggaacta
    37201 gaggtagggg atgcatctgt gggaaaaatt gcttttataa attttttaaa acgtttttct
    37261 ttaccttatc gtcctgctaa gaacaagatc caacgtatgc ttgaagctaa aaattagaaa
    37321 cctttcattt tcacaaggca cttgagttca tttttttact tttttctgtt aagttatagg
    37381 atgtgaaaga atctagtcac tagttgtata gaatctattt tgggggagat tcatatgatc
    37441 gaaatctatt tatttgttaa tcctttaggg gaatactgtt tcgaaatgga acaacagtta
    37501 ttacaattta tcgaagaaga gtacgggaca ccatcaaaag aaaagatgca atttcgcttt
    37561 cttcctttgg tcaatttgca gacaattggg gatgtcatgc aacaaaaagg aatttctcag
    37621 aacaatcttg aggaacgtaa tcacttattc tcaatgactt attctgcagc attggactgc
    37681 aaagcggcgc agtttcaagg aaagaaaaaa ggccgccgtt ttttgatcaa attgcaagaa
    37741 gcagtcggct gtaatgacat accatattct caagaacttg ctgaatccat atttgaagaa
    37801 atcggcggcg atttgaatat gtttaaagaa gatcgtcagt ctgacttcgt taaagaagta
    37861 ttcttttctg atcaaaaaat tgctcgggaa atgggcatca caaaacaccc ttctgcagtc
    37921 gtttacaatt atgcctgcga ccgtgatttt ggtgttttag tagaagtaaa aggtacaagt
    37981 agcctttgga gaatcacaga gctgtgtaaa acaacagata actcatatca agtttttaat
    38041 ggtgaacatt tacatacaaa tgattctcag ttccattcaa gaactagcca tttacacttg
    38101 ctttctaatt aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt
    38161 tctattcgag aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc
    38221 atttagacgt tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc
    38281 aactcctgct tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc
    38341 caagtagttc tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc
    38401 agcagaaaag ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg
    38461 tgaccattcg aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt
    38521 caatttacca taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc
    38581 cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa
    38641 cccatccaag taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa
    38701 taaatactcc gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga
    38761 gtagtcgccg tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc
    38821 catttcgtgg acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata
    38881 tggatttgtg tcatagcttc ccgaagaata agcatcgctt cgtttccctt tattttcgac
    38941 aacatcgatc caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat
    39001 tggtttcagt gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc
    39061 ttcccctaag accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg
    39121 tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa
    39181 agtatcgtat acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct
    39241 gtaattacgg actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc
    39301 aaatgtattt ctaaattgtt cgtaaacact gtacaatccc ttaaatgctg cttcgcgcac
    39361 ccttcgatcc gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc
    39421 accattttct ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt
    39481 atcacttgaa gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga
    39541 taagacatgg gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt
    39601 tggttcttct ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc
    39661 aaaccaggaa acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc
    39721 ttggtaatct gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac
    39781 ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc
    39841 ttgatcaagt gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata
    39901 tttctcatca aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc
    39961 aggcaattct tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc
    40021 cttagctcta tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact
    40081 tttctactca ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca
    40141 tagaaaaccg attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta
    40201 tgcattcgtt aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt
    40261 cccttttatg ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt
    40321 ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa
    40381 accgactttt ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc
    40441 attgttcttg tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt
    40501 tcttcccatc ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac
    40561 tagtgaatat ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa
    40621 tatgccctga tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat
    40681 cagctttcca gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca
    40741 atgagaattg cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt
    40801 tattcaaata attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga
    40861 tttctattgc tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg
    40921 cttccaaaaa tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact
    40981 gtaagtgctc ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg
    41041 caaaatgagc acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac
    41101 aataatattt ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca
    41161 ttgcaagcag catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa
    41221 aaaagagggc gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag
    41281 ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt
    41341 tatacgaaaa aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt
    41401 atacaaaata aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt
    41461 cattcaatgt ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct
    41521 gattctctac atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc
    41581 gctcttcgaa taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt
    41641 ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg
    41701 cgcttccaga taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac
    41761 tcacatcttc tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat
    41821 ttttactgat aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt
    41881 cgcttcctcg gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt
    41941 cgatttcttt gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt
    42001 catgaccaat tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac
    42061 acctcacttt gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa
    42121 agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca
    42181 agctatctta tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag
    42241 ctaattttcc ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct
    42301 aatcaagaat actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagt
//
LOCUS       DACDEA010000017        41907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDEA010000017 DACDEA010000000
VERSION     DACDEA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.017"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..1368)
                     /locus_tag="IV136_000920"
     CDS             complement(118..1368)
                     /locus_tag="IV136_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8464928.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            1777..2571
                     /locus_tag="IV136_000921"
     CDS             1777..2571
                     /locus_tag="IV136_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8464929.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            2665..2967
                     /locus_tag="IV136_000922"
     CDS             2665..2967
                     /locus_tag="IV136_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464930.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(3003..3374)
                     /locus_tag="IV136_000923"
     CDS             complement(3003..3374)
                     /locus_tag="IV136_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464931.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(3376..3777)
                     /locus_tag="IV136_000924"
     CDS             complement(3376..3777)
                     /locus_tag="IV136_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464932.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKIK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(3791..4198)
                     /locus_tag="IV136_000925"
     CDS             complement(3791..4198)
                     /locus_tag="IV136_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464933.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            4542..4991
                     /locus_tag="IV136_000926"
                     /pseudo
     CDS             4542..4991
                     /locus_tag="IV136_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5121..6283)
                     /locus_tag="IV136_000927"
     CDS             complement(join(5121..6007,6007..6283))
                     /locus_tag="IV136_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8464934.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            complement(6960..7033)
                     /locus_tag="IV136_000928"
     tRNA            complement(6960..7033)
                     /locus_tag="IV136_000928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6997..6999),aa:Ile,seq:cat)
     gene            complement(7223..8233)
                     /locus_tag="IV136_000929"
     CDS             complement(7223..8233)
                     /locus_tag="IV136_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8464935.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            complement(8230..8619)
                     /locus_tag="IV136_000930"
     CDS             complement(8230..8619)
                     /locus_tag="IV136_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8464936.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(8719..8859)
                     /locus_tag="IV136_000931"
     CDS             complement(8719..8859)
                     /locus_tag="IV136_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8464937.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(8852..9199)
                     /locus_tag="IV136_000932"
     CDS             complement(8852..9199)
                     /locus_tag="IV136_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464938.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(9213..9707)
                     /locus_tag="IV136_000933"
     CDS             complement(9213..9707)
                     /locus_tag="IV136_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464939.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(9707..10156)
                     /locus_tag="IV136_000934"
     CDS             complement(9707..10156)
                     /locus_tag="IV136_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464940.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(10160..10780)
                     /locus_tag="IV136_000935"
     CDS             complement(10160..10780)
                     /locus_tag="IV136_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464941.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(10780..11688)
                     /locus_tag="IV136_000936"
     CDS             complement(10780..11688)
                     /locus_tag="IV136_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8464942.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(11701..14475)
                     /locus_tag="IV136_000937"
     CDS             complement(11701..14475)
                     /locus_tag="IV136_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8464943.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            complement(14475..15257)
                     /locus_tag="IV136_000938"
     CDS             complement(14475..15257)
                     /locus_tag="IV136_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8464944.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            complement(15275..18097)
                     /locus_tag="IV136_000939"
     CDS             complement(15275..18097)
                     /locus_tag="IV136_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8464945.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            complement(18114..18401)
                     /locus_tag="IV136_000940"
     CDS             complement(18114..18401)
                     /locus_tag="IV136_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464946.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(18455..18823)
                     /locus_tag="IV136_000941"
     CDS             complement(18455..18823)
                     /locus_tag="IV136_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464947.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            complement(18869..19381)
                     /locus_tag="IV136_000942"
     CDS             complement(18869..19381)
                     /locus_tag="IV136_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP8464948.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(19392..19781)
                     /locus_tag="IV136_000943"
     CDS             complement(19392..19781)
                     /locus_tag="IV136_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP8464949.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(19778..20149)
                     /locus_tag="IV136_000944"
     CDS             complement(19778..20149)
                     /locus_tag="IV136_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8464950.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            complement(20146..20460)
                     /locus_tag="IV136_000945"
     CDS             complement(20146..20460)
                     /locus_tag="IV136_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464951.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            complement(20457..20786)
                     /locus_tag="IV136_000946"
     CDS             complement(20457..20786)
                     /locus_tag="IV136_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP8464952.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            20836..21123
                     /locus_tag="IV136_000947"
     CDS             20836..21123
                     /locus_tag="IV136_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464953.1"
                     /translation="MCDPSSVFIVVVYLPAGTVSVQVILPLEETVNPLVTGAIEYVTV
                     LFVAFCGATVAVNCLLPAVPATALEVLGVTVTPITGIVTPPPVQFYRTQHL"
     gene            complement(21080..21967)
                     /locus_tag="IV136_000948"
     CDS             complement(21080..21967)
                     /locus_tag="IV136_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8464954.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            complement(21982..22584)
                     /locus_tag="IV136_000949"
     CDS             complement(21982..22584)
                     /locus_tag="IV136_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8464955.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(22887..23114)
                     /locus_tag="IV136_000950"
     CDS             complement(22887..23114)
                     /locus_tag="IV136_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464956.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            complement(23192..24148)
                     /locus_tag="IV136_000951"
     CDS             complement(23192..24148)
                     /locus_tag="IV136_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8464957.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            complement(24153..24416)
                     /locus_tag="IV136_000952"
     CDS             complement(24153..24416)
                     /locus_tag="IV136_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464958.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            complement(24403..25899)
                     /locus_tag="IV136_000953"
     CDS             complement(24403..25899)
                     /locus_tag="IV136_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8464959.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            complement(25912..27156)
                     /locus_tag="IV136_000954"
     CDS             complement(25912..27156)
                     /locus_tag="IV136_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP8464960.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            complement(27143..27676)
                     /locus_tag="IV136_000955"
     CDS             complement(27143..27676)
                     /locus_tag="IV136_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP8464961.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(27703..27927)
                     /locus_tag="IV136_000956"
     CDS             complement(27703..27927)
                     /locus_tag="IV136_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464962.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(27934..28146)
                     /locus_tag="IV136_000957"
     CDS             complement(27934..28146)
                     /locus_tag="IV136_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464963.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            28629..28808
                     /locus_tag="IV136_000958"
     CDS             28629..28808
                     /locus_tag="IV136_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8464964.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(28801..29214)
                     /locus_tag="IV136_000959"
     CDS             complement(28801..29214)
                     /locus_tag="IV136_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464965.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(29475..29891)
                     /locus_tag="IV136_000960"
     CDS             complement(29475..29891)
                     /locus_tag="IV136_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8464966.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(29967..30263)
                     /locus_tag="IV136_000961"
     CDS             complement(29967..30263)
                     /locus_tag="IV136_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464967.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            complement(30287..30592)
                     /locus_tag="IV136_000962"
     CDS             complement(30287..30592)
                     /locus_tag="IV136_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464968.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(30608..30805)
                     /locus_tag="IV136_000963"
     CDS             complement(30608..30805)
                     /locus_tag="IV136_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464969.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            complement(30802..31101)
                     /locus_tag="IV136_000964"
     CDS             complement(30802..31101)
                     /locus_tag="IV136_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464970.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            complement(31098..31682)
                     /locus_tag="IV136_000965"
     CDS             complement(31098..31682)
                     /locus_tag="IV136_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8464971.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            complement(31679..32092)
                     /locus_tag="IV136_000966"
     CDS             complement(31679..32092)
                     /locus_tag="IV136_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464972.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            complement(32089..32334)
                     /locus_tag="IV136_000967"
     CDS             complement(32089..32334)
                     /locus_tag="IV136_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464973.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(32294..32650)
                     /locus_tag="IV136_000968"
     CDS             complement(32294..32650)
                     /locus_tag="IV136_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8464974.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(32650..32955)
                     /locus_tag="IV136_000969"
     CDS             complement(32650..32955)
                     /locus_tag="IV136_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464975.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(32952..33113)
                     /locus_tag="IV136_000970"
     CDS             complement(32952..33113)
                     /locus_tag="IV136_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8464976.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(33128..33487)
                     /locus_tag="IV136_000971"
     CDS             complement(33128..33487)
                     /locus_tag="IV136_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8464977.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(33484..34335)
                     /locus_tag="IV136_000972"
     CDS             complement(33484..34335)
                     /locus_tag="IV136_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8464978.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(34352..35182)
                     /locus_tag="IV136_000973"
     CDS             complement(34352..35182)
                     /locus_tag="IV136_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8464979.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(35185..35871)
                     /locus_tag="IV136_000974"
     CDS             complement(35185..35871)
                     /locus_tag="IV136_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464980.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(35877..36548)
                     /locus_tag="IV136_000975"
     CDS             complement(35877..36548)
                     /locus_tag="IV136_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8464981.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGDFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(36541..36882)
                     /locus_tag="IV136_000976"
     CDS             complement(36541..36882)
                     /locus_tag="IV136_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464982.1"
                     /translation="MENELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(37208..37396)
                     /locus_tag="IV136_000977"
     CDS             complement(37208..37396)
                     /locus_tag="IV136_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464983.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(37417..37701)
                     /locus_tag="IV136_000978"
     CDS             complement(37417..37701)
                     /locus_tag="IV136_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464984.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(37679..37891)
                     /locus_tag="IV136_000979"
     CDS             complement(37679..37891)
                     /locus_tag="IV136_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8464985.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            complement(37894..38670)
                     /locus_tag="IV136_000980"
     CDS             complement(37894..38670)
                     /locus_tag="IV136_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8464986.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            complement(38796..39017)
                     /locus_tag="IV136_000981"
     CDS             complement(38796..39017)
                     /locus_tag="IV136_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464987.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPREHQKNSFATG
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            39315..39662
                     /locus_tag="IV136_000982"
     CDS             39315..39662
                     /locus_tag="IV136_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8464988.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            39698..40093
                     /locus_tag="IV136_000983"
     CDS             39698..40093
                     /locus_tag="IV136_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8464989.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            40147..40458
                     /locus_tag="IV136_000984"
     CDS             40147..40458
                     /locus_tag="IV136_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8464990.1"
                     /translation="MIYTEFKEWLEKNTTGYETFIVKATNYQIEKNKNRPPKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            40470..41618
                     /locus_tag="IV136_000985"
     CDS             40470..41618
                     /locus_tag="IV136_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8464991.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT        12084 a         8606 c         6638 g        14579 t
ORIGIN      
        1 agatgaaatt taagattttc tttattgagt aatatgtttt gaagcgcacg aaactagacc
       61 cgatcaaaat caaatttttt tcgggttgat tcaaggattt gcgtcaataa cggattgtta
      121 tttctctatc acactccttg gtggcgtata gagttggtgt ttacgtagta gcgtatccac
      181 cagacgcgta aattttcttg cggttaggac gagtgcacgt ttgtgttgat gctttggaac
      241 ctccttatat ttactttgat aaaaagcttt gtattcagga agataatttt ttacagagtt
      301 ggcagcttca actaagtagt atctaaaata tcgatttcct tgttttgtca gaggtgtatt
      361 ttgcgactga tagtttccag attggttcac tttccaactt aatccagcgt attttgccaa
      421 ttttgtctga tcttcaaatc tttcgatttg accaatttca gcgattaaac cggcagcata
      481 gactttacca acgcctggaa tactggttaa gcattgatat tctggaatga caacaattac
      541 ttgttcgatg gctttatcta attctttgat atttttttca agtgctcgta tttctcgtac
      601 gagaacactt aaaacaacat taattgattc ttgtgcaaga gcacctaggc ggtaactcat
      661 ggtaattgca cgttgaattg ctttagcgat tttttctggt tgcttgaaac ggccttttcc
      721 tttttcttga agtaaatcac agaaagcttc taaaggaagc tcagcaagtt catccaatgt
      781 gaaatcttcc gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg attcgtctcg
      841 catttctcga gcaagcgtat tacatttgta cgttagattt tcaagaaaat gttgctttgt
      901 acgaatcaat tgcttaataa gttgatagcg agtacgtgtt aaacgctgta aggccatgta
      961 tttctcttct ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat cagcgattct
     1021 taatgcatcg tttcgatcag ttttattctc atcaaacatg cgactaaatt gtttcacccg
     1081 aaaaggattt tcaatcgtta caagtgtgtt gagcttcttt agtttctcat cttcatgaaa
     1141 aaacatggaa ggatggaagc tgtacatgga cgttgactcc atgccaatca caatttgatc
     1201 aaagtggtag ctgtcattga attcaagaat tgtttctcgt atgaatgaag caccttcgat
     1261 gtcattggca acagagattt cagataagat agacaattga ttgtcgtctg ttaaaaaaca
     1321 aacatctagt ttttcagagc taacgtcaat accgacaaat aatttcatgg aagaaggacc
     1381 tcctttcgat ataaattttg gaaaatgctt cgatactgtg ggatttccca gaaactcact
     1441 gtatagccac aacctcgcct aatagaatac ggtgataaaa gagctagaag ctgccttcca
     1501 aactactatt tgaaagtcta gtccattatc gaaacagcta atgagttggt agtgataaca
     1561 gtggttcggg taacagacta aacagggtag acaaacgcta caggaggaaa tagcaaattc
     1621 ccgttggatc ctttccatac atccattgta ctctgagaaa tcacacaata tcgaaacaaa
     1681 ttccgttagg aatgtgttat tcaataaatt ttataatcta gaaacatttt gaataaaaac
     1741 tattagacaa atgatatttt acgaggagga actaatatga gtaaacaatg tgcaaagtgc
     1801 ggaggtaaaa ttggcttaac ttcatataaa atcaaagata aacaaattgt ttgcggcaat
     1861 tgtatgaaaa aagctggtta cggtatgact actccttata aaataataag atctttgaat
     1921 ttagaagatt tagataaggg tccatcagaa aaaagaaaat taaacatatc tgattatact
     1981 acacaaaaca atcatgatat aggtaatgat agaatgagaa ctactgaaga gatggtagaa
     2041 ttttgtctta aatacggcta tggtaaagga atgactaaaa aatggacaac tcatcatttt
     2101 aacttaatat cagatcaact taacagtgac gaatacgtaa tcttctgctt tgtaggattg
     2161 cataactata tctcgtcaac taagcatgac aataattatg catacgcatt aactaataaa
     2221 cgtttaattg tagctcaaca aaaaatgata ggcaataatg tacaaagtat tatattagac
     2281 aatttaaata atattagtaa aaaaagagaa atactgttgg gtacattaac tattgatact
     2341 cttggtaaag tattgaatgt tgcagttgat aaagatactg ttgataggat aagtgattct
     2401 ttaaacgaaa ttatttataa tttaaaaaga agaaatattt ctgctataaa ttccaataca
     2461 tcaacaattt cctcagcttc agaaataaga aaatataaag aactattgga tgacggaatc
     2521 attacagaag aagaatttaa taaaaagaaa caagaattat tagatttata atatctatca
     2581 aaaaagatga gcatttaatt tagctcatct ttttctattt acaatacaaa catttgttcg
     2641 tatactcttt ttgaggtgaa cattatgttg atggaaggaa aaacacaatt atggtttaaa
     2701 tttgatcctt cgaatagatt tatcaaagat ttttataaag tatggaattc agaagttttc
     2761 tttttagcaa tcgaagatag cttattaatc aatctctact attctaataa gaactatttc
     2821 aaaattcctg ctgcgaaaac tagaatgaag aagggtgtat actttttgtt tgatatcgtg
     2881 actgacgtgc cggacgcacg tagcgatcat cggcgttatg actatataaa gtatactttc
     2941 gttgatccag aaagatacaa agattaaata aaaaaataac aaacaaactc gcttgaaatt
     3001 cattaggtga gtttgtttat tattttttct gcttctcgat tcatcagcgc catatctgtt
     3061 ttcgtaatgg tttcattagg aatatagtca gcacattttc ttttctctaa cattctatac
     3121 ccactattat gtattcctaa ggtattcaat tccttaactg ttgaaaacaa attccaaacg
     3181 gtttggtggc ttccttttcc gctacttcca ttataaataa tatcataatt ttctttcaaa
     3241 tactcatcag attttttaaa agcactgtag tatgctcttg aaacagcagt tcttctataa
     3301 gattctttag aattatattt atcatctgat tcaatttgtt tggaaataga taaatattca
     3361 ttaaagtcaa acattttatt cccccaaagt tgctactaca gatttcccca tatatttata
     3421 gtcagataat acattatcga gagtatccaa aacatcgtaa tctgaggaaa acaattcaga
     3481 tgttgttttg ataacaaaga aatacaagtt taaatcttca tcatatttta tattgatttc
     3541 agcttttctt tttaaaaccg tatttatcac atcaaaaatt ttgccatcat tacttgaaat
     3601 tgtgttaatc tcattttttg tagtaattac aagttgatta tcatttttaa ttttcggagt
     3661 agaacttgca tccacaacaa ctggagatgc tagaattacc gatgcgcaag caaaaatcgc
     3721 agcaaaattt attaagctac cactccgatg tgtatttgat ggtactatat caagcatccg
     3781 tatcgtctcc ttaatttttt tccactggtt tgttgacctt gatatcaaag atttgccttc
     3841 caatctcagt tccattaaat ttaacaacag cataatgctc tcctgcttct tcaaccaaga
     3901 catttctggc gtttatatta aagtttatat cgttaacttg aggaggcaag cttaccgata
     3961 cttcacccgt agaaaaaatt tgtttttcat tcttgtttaa aacttcaatt tctactattt
     4021 ttatcttttc tgtatcctct atctcagaca ccacagctgt tacagaaaat gataaagcag
     4081 taggaattaa aggcgcatta atagaaagag ctggattaat tactttatta ataacattag
     4141 tgccaaattg ctgactttct acatcttctg aaattattaa ttgtactcta taagacattg
     4201 atttctccta atttttacat ttatttaata aatttatgtt tcacttttgc tagtcaattt
     4261 agtttaacaa tagtaacaaa gctttgtcaa cggaaatatt agctaatttt gtaagcatat
     4321 ttataagtat atttgtaagc atatctgaca tttaatcagt agtaaaagtg cctctatata
     4381 aggaacaaac tgttttttta ctatctaaat tcgtatacag ttttactaac aaattagcta
     4441 acaatattag caactatatc aacaactata tgtacttccc ctcaaaaatg atgtgcaccc
     4501 caaaagttag actagaaatc taattaaaag gggtgctttt tttgcggaaa tatacatttg
     4561 tttttaagaa aaaagtagtt tcagactatt taaataacga aggcggctac aaatatcttg
     4621 cacataaata tcaaataaat cgtacactgg ttagacattg ggtaaggatt tataactatc
     4681 atggttggga aggcttggtt ggaggtggca aaagctacac tacaaaattt aaacttgatg
     4741 ttatagaata tatggaaaca aatggtcttt ctatccaaga aactgctaaa aaatttaata
     4801 tcggttcaaa tagaactcta agtaaatgga tagagcaata tgaagaaggc ggtgcttctt
     4861 cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa ttccaagcta aacattccta
     4921 aaaaacttaa agatgagtct cttgaagaag aagttattcg tttaagagca gagaacgcat
     4981 atttaaaaaa gtggaatggc aacacttttt ttgacaaaaa ttataaggtg ttgtgaatgc
     5041 tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg attttctccg
     5101 gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc gcgctcatta
     5161 tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc atacgccaac
     5221 gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc tagacgtagc
     5281 tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga tttataggtt
     5341 gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg agtaattcca
     5401 aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg gtctgtatga
     5461 aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt taccaatgag
     5521 gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa atcaagtatt
     5581 aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt aacgatggct
     5641 tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt cgcttcatta
     5701 caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa tccgcggcgt
     5761 ttcatgattc gaccgattcg gcgccgactg agttgaagcc cacgctttgc taaacacttc
     5821 ttcaattttc gggtaccgta agcctttcgg tttcgaataa actcttcctg aacgatctct
     5881 tctaagtcgg actcattttc gatcggtttc gcttgataat agtaggtttg acgagaaata
     5941 tttagaattt tgcacatcgc tgatatggaa tatttatgct tgttggcatc aattacttgt
     6001 cttttcgtcc gaatatcagc gccgcttgct ttaaaatatc attctccatt tcgagttgct
     6061 tatttttctt tcttagtgcg atcaattctg cttgttctgg tgttaagttg tctctttctt
     6121 tgaatgagcc cgttgattgt gcttgcttca tccatttatc gaaagaagaa ggcgtaagct
     6181 catactctcg aataatttct gcgcgaggct taccagcgag atagagatcg acgatttgtt
     6241 gtttgaattc ttttgaatag gttcttcgtt gacgtcttga catgaaaaat cctccagtgt
     6301 gttttttatt attctacaca ccttattttt tctgtctagt ctagtgtagc cgattcatga
     6361 ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg
     6421 ttgatccaga aaggtataaa gattaaagta ggctacctaa aaaggtagcc cggaacgaat
     6481 tttatcacca tacttgtgaa aggagatatt ttttaagtta gtattaagat tgtgtaatat
     6541 gatgatatct atattttata gtatcagtgc tataaaatca aaaataagtc actaattaac
     6601 taccactcca attgtaagcc ttttttctca ctttttttca aaaatatggt atgcttttta
     6661 atggcttcaa atataaaaga gtttaaagcg taacacactt atggggaagt ggtttggggt
     6721 gcgctttaaa ctcttcttta ttattatctc acaatttaac ccaaatgtct ttctatttaa
     6781 aaatcaaagt aaaacttttc aaatatacag aagtataact atgtgaaaca tcctttcatt
     6841 aatccataaa aggatacata aaaaagccac tcatttgagt ggcaatgaag aaaagcttta
     6901 gcttgtataa tactcttcaa aaaaattcta acacagaacg attcaaatgg ctacgttaat
     6961 gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct ctgaccaact
     7021 gagctaaggg tactggttgt tgccacataa agccataaac aatcaaccag tagaatgtgt
     7081 ggcaacaaac ctgttatcgc atagcttgga gtgtgactat ttatgggtga tagtgaagat
     7141 atgcgataac atcactattt tatcgaataa tttttatagt tgtcaatata gttatgtact
     7201 gctcctcaac gaggagctat ttttatcgtt taggaatatt taaataccaa cgtttgtcat
     7261 ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc attcgttgcc cgcatcatta
     7321 catagacttt cttactagga aaattacgca tattgaaaga tacatgataa ccaacatttc
     7381 cagaagtatt ataagcttga tttacatccg gtctataaat tccatcagct cttactcgag
     7441 ctaattcttt cccagtattg tagtccataa tgaaaatata ctcgtattta tagttagcga
     7501 tgtgccatcc agcgacgtgt aagttcgcat tttcgatttc tccaaactga tcaatgtgag
     7561 aatggttcgt tccatctgat aaagttggat ttgctgcacc tgctcgggtt ggatcaatga
     7621 caggcttgtt ctcagaagtc gttgggtttt cgtctgtaaa tccatgagct aaatcgtaag
     7681 ccagtttttc cttacttaca cccatttctg ataagtatcc atacggatca gtgtgatcgc
     7741 cccaaatgtt ttgagttacc cataaatgtg atttgattcc tggttgatta tacggtgtgt
     7801 ctaatgtaag tggaataccg tatttattag cggaatctct cgctaattca acatatgcct
     7861 tgtagttctt ttcaaacgtt actttatcgt gcgtatgttg cagttcgatt tgcacaggac
     7921 tatttgcatt agcatatgaa ccagcgccat attgtacata accaggttgt ccaacttggt
     7981 aaacgatccc accatcgcca acaatatacg ctgtataggc attgtaccaa gaacgtttca
     8041 tgtactgcgc ttcatttcgt cctgtcgcag tttcgtttgc tgtttcatgc aataggatgt
     8101 attggttatt tgctacttga gaactacctt cgtttgcgcc caaattgaat tcattgttga
     8161 tcgagtaagc aaaggcgttt attggcaata aaaaaagagc tataatagct cctacaaaga
     8221 ttttcttttt catttggttc ctcctcggtc tttattttca gtatcatttc gtatttgttg
     8281 gaatacctct tttaacttat ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact
     8341 aatcccttca tttgcaacat agaacataat aactatttct cttagaggta tagaattccc
     8401 aacgattttc tgtacgtcaa ccgcaacggc aatcacaacg aagattaaga ctttcttcaa
     8461 caatccttcg aaacctatct cactacttat ttctttcaat ttccatgcct tagcaagtcc
     8521 agttaagaag tccacaccca caagaaaaat taaaacatgt agtatattgt ccatccctcc
     8581 caataaacca acaattgtcc cgcctatcac ccctgtcatt actgagagtg tatttaaata
     8641 tttttccaat atctatttct cctttatgca aagaagcgca ctcgtttgag tacgcttttt
     8701 atttaacttt gtgcttaatt aaatttcatt tccagtaatc tgcttatatt ggtcttttgt
     8761 taaacagttc atgtttacat agtcaagtaa atcttggtcc gtataacatc cccaatcata
     8821 aaattgtttt atattttcaa agcccggaaa actattcatt acttttccct ccattcttaa
     8881 tacttgcgac ttccatcgtc aattgagcta acatttgttg cattttttct tgttcctgta
     8941 ccgatatcgc caattgtttt gtcagtagag catttaattg cttcaactga tcgacatctt
     9001 caatatcgtc aaccgctgtg ccactcttct cttcttcagt agctaactca acccactgtt
     9061 ttttatcaaa atcaaatctt ggtctccagt taggaactgg tggtttaatc tctgtacagt
     9121 tttcagggat attttcttga ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg
     9181 atttgtaaac tactttcatt agatttcctc ccttaaattg aataagtgat aacaaatgaa
     9241 taagctgatc cataacttga atttcttctc catttaatgg ctccgtctgc gccaatagat
     9301 agttgagcac tgttcaaagt agaacggtct atcgagccaa ccagttgctc aaaactaatt
     9361 ggtggccgat agccttctgg aattgttagt atcgttgaat catttccacc actgcttttt
     9421 ccgtttaaag ccacaaaata tatagaaact gtttttcctt cacgataaag ctttgctgtc
     9481 cctgtattac cgttagtgac aaccagattt accacttcat atctgttatc atcagtcgtc
     9541 aaaacacgtt tattattgat tgttacagaa cctgaaaaat tcttatgtcc tgcaatggct
     9601 tggtctccat ctgtctgcac caatgcttct ttaccatcaa tagcttttac atgcgttttt
     9661 aagtatttag caactccgtc ttcttttaat tgaacaatat cagccattaa accgtcccca
     9721 ctttctcaaa tgtaatattg gctaatccat caagtttcac tttatcggtt gctgacatta
     9781 aacctgctgt cgtagttgta gcattacctg gatttttctg tgctccagct gcaattccat
     9841 ccaacttagt tttatctgtg gacgacatca acccatttgc tgtagttgta gctacagctg
     9901 tagttgtggc atttattcca ggatcacctt tatctccctt tggtaaaaca aaattaaatc
     9961 tagctgcaga tgatgttcct acattcgtaa cagaagcggt tgaaccacta gaaacggttc
    10021 ctatggtaat tgttgctgct tggccaggat cgcctttatc tcccttcacc gttgttggtt
    10081 tgccttctat agcattccaa tgagtttgtg gataaacctg tacattgctt tgttttattt
    10141 ttacgatatc tgtcattctc tatacctccc caattttttc aaaagtaaaa tttggaattc
    10201 tttcgtttgt gtaattttct gcttgattta cagcttcttg gaatttttga tctacatatg
    10261 actgattgac accaccagtc ccactaccac ctgtagaact aattgttcca tcttcatcaa
    10321 tagtgatgtt ttctccagca gtataatttt tcagtgaatc gagtttttct tttaactcca
    10381 gcgaaaaatt gacatcagat tgtctgactg cagagacttt ccctgtctca tcaatcgtca
    10441 ataattcacc aacttttatg ccacccaaac ggtcgatagt ggcaactggc aacacgtagt
    10501 caccttctcc actgtttaaa attttttgat acatttctgc cgtgataatg ccgtcatctt
    10561 cttcacttgc atagggtaat tctgtgagaa cattctctaa tcccaaatct tctttagtaa
    10621 tgacgacggc tccaattttt ccattaacag ataaaactgt tgcttctcct gctattattt
    10681 cttctaaccc aagaactgcg gaagcatgtg tgattggaaa gaattggcgt tgtacacccg
    10741 ttttttcatc cgtttccatc attcgtttag ttttagccat tagatcaccc caactttctc
    10801 aagagtgaac acattttgct ttggatcatc aacggttgcg atgatcaagg ctccttcttc
    10861 aatcgggaat gctacagttc caactttttc gacttcatga ttatctgaaa agagatcatc
    10921 ctgcaaaata tcagtcactt caatctcccc gtattcaatc gtgaaaagcg ttgctcgcaa
    10981 tttctgatat aaatagtcca tatctgccag caaacgctct gaaatagacg catgacgaac
    11041 tccttgaata tctactcgcg catccgttag ttcggctaac atgactccac ctggatcaat
    11101 cgtctttaaa atatccttga ttgattcaaa ccatgccaaa tagtctgatt cttgtccttc
    11161 tctccaatct tggaaactgt gttcctgttc ttgtctccaa cgttcaaatt cttctttcct
    11221 ttcattcatc cagtccgtaa aatcgccttt attttcatta ataaagtccg tcatatccgc
    11281 aattaaatct tcgattgatt gccaataaga acccatttct ccttctgttt ttgaaacagc
    11341 attaatgaca aaataagaga agtcttgagt tgttccaatc aagtcttctc ctttaaatat
    11401 aatgaaattg gctgtttgtc gatgtaaaca ctgcatggaa tatttatcaa aaatatagtt
    11461 aatttttcct tttttagcat ccacaatttt tgtttctaat tggactggat atttcccacc
    11521 tacaactgat tcaaaatata ccttacattc tgataaatcg tagggaagac cattttcaac
    11581 tatagtcgct tccataacct ctgtattctt attgccttgt cgaacttgaa tcatccccac
    11641 ataattatat ggttctgttg tacttaaaac gacattccat ttactcatag aatcaccacc
    11701 ttaaaattgg atataatttc ttggattctc aaaatcaaca actggctgtg gccaataatt
    11761 tttcataatt tgaaaatgta gatgttctcc tgtagatgga ccagttgttc ccatcaagcc
    11821 tatttgagca cctttctgga ctttttgtcc aacagaaaca tcaatacgag acaaatgagc
    11881 ataacctgtc cacttcccat tattatgttt aataaccacg taattgccat accagtcata
    11941 ataattagca ccagcctgaa caacttcccc atctaatgct gcaaaaacag gtgtattagg
    12001 atttccattt acaagatcaa ttccattatg aaattcttgg ccgccattta ttggactagt
    12061 tctccatcca cattctgaag taactgtcac tggtttttga attggacaaa tgaaattccc
    12121 tccaggttct ggactagtta atccatgtaa tttgttatac caagattggg cataattttg
    12181 gcgttctgga tgtgcagaag ctgggcgttc gaagttcatt tcaaaagcat acgctgcact
    12241 tttggcatca ttgatcgatt taaatcctga tactgtggtt ggatttactt ttccgagcca
    12301 ttgaccgttg aacatacacc aatctaataa ttttacttgg gcttcaatac ttcgataatc
    12361 ttcttgaatc cctgcggtgt tcatcaaacg ctgaacatac tctcgtccgt tccaagttgg
    12421 tgatccgact aacggataag cagaaccatc ccattgaaca atcccataag ctggaccacc
    12481 aagttgttct gtatcagggt tcatacttgc tccaacctcc ccctgaatat ttcctagaat
    12541 acctgcagct gcatattcag aatagccacg agctttcagc attgtccaaa ttttccatgc
    12601 tcttttttca gcttccgtcg tcaattgagg agggatagtt ccaggatcac ttcctccact
    12661 gttgttccct gtgatttctt ttccgttaac tgtgagtttg ccttgtacat ctaagtctcc
    12721 aaaataaatt gctttaccat ttcctaataa aactaatcct ttaccaactt taggagaaat
    12781 caaaatatat ttgccgtctc catttgtacg aataactaaa gaattatctt caataggtgt
    12841 gggagtagaa gctccaggaa aaggattacc agcagaatca gtagtaccaa ttgtgccaat
    12901 ggattctttg gtattccaga actccatccc ttttttggtt aattccatga tttttttatt
    12961 gctgtttaca atttgcaaag ctccttttac taacttgagc tgatcaccaa cagcgttaaa
    13021 tgaagtttca aaaatatctg ctctaatacg tccagattgg atataattgg cattgaagtt
    13081 tccatcaatc gtccatgccg ttttgaaatt tgactcattg aatttcccat caataaaacc
    13141 aatcccttcc gaattagcta caagaaaatg gttagaagta tcaatactat ccccattcat
    13201 ccaaaccatc tgatatggtt gacgactttc gcctttgcca gtatcccaag gattcatcat
    13261 gataattgat ccaccctttg ctccgcgaat gatatccgac tgccatttag aaacttctgt
    13321 ggactcataa aaggtcatct tcttctcatc aagggtctgt atggcagttt gagtatttga
    13381 agcttgacgt gttgatgaag tatttaaatt atcacctaag ccagtttcga cttttcctgt
    13441 taatcgatca atcttcacac tgaaaattct agtctcgtaa tgatagccgc ggtcatcacg
    13501 atgaatacgt ataatattac ctaaactatc tgctccaaaa atagttgctt taaattgaac
    13561 tagcggacgt gaacagttaa ccaattcttg ataggtaagc tgaattagtt caacaggatc
    13621 ttcgcagtct tcgaatataa ttaccttctc acgcttacga cgttttccgt ttttagtagg
    13681 aataccatat ttttcagtca tctcagggat ttccaaatat atctggccag taggcttgtt
    13741 taacggatcc ccttttgatt tggaccagta tacttgatca aactcaattc gacgaccata
    13801 accatctcca acctcttcac cacggccgcg gccgattaga gaagtataga tgttacttcg
    13861 atctacttct ttttcaattg tcaaggcttt atcgccgtac tcatatcgct cattactgta
    13921 ttcgccaatc tgcttgtaaa cttctatcca tttatccgtg attccttctc cacttagatt
    13981 gcacctaaaa acaatctcgc atcctaacgt ttgtagattt tttaaggctt cacggatact
    14041 acagtagtaa aaagtcatag aaaccgctgg cagagttgaa tctaaatgac caactcgcca
    14101 ctcacctaat gtaaaatcta tgactctctg gatggttttc tgaaaaaatt cgttagccgg
    14161 acgaatatca ttaatgatat aagcatccaa ttcatcaggc ccaaaattaa tcccagtgaa
    14221 tatcaacaat gaacccggat cagcaattcc gataatttta tacatagaaa aagacgactc
    14281 gctttcacga accgccatat aagctgactc tttaatttcg tcatcaaact ccatgctaac
    14341 tgctaactta tcattaatta actcatcttt acttggtgtg atttcttttt cttgaactac
    14401 cgaaaaaagt ttatcttccc ctactatttt aataagcttt tggctatcgt cgaaaaaata
    14461 aacatctttg tccattacag ccacactcct ctatatcgta ttaagggagt gccattatca
    14521 cacttaacta gatcaccagt tctaaccata aagtttttaa atgagctggt aagatcaaga
    14581 attctcgttt tattcactcc attcacaaaa acttttcctt cagctatgtc catctcgata
    14641 aagtcacctt tttttatcat tgagttagtc acactaatat tttgacggcc atttgtaatt
    14701 cttaagctcc catcattatt agcctttaaa ctaattgaaa tcggagctac ttcatacgga
    14761 agatattctg agactatgcc gtttgtttca aatatacgtg tatattttct gggatcagaa
    14821 cagattatag agaaactaga tattacacta tgaacgtttc ctggaatatt atcacaggtc
    14881 tgataccttc cgtaataata catatccaac tcatcgttaa agtgaatttc tacatcttta
    14941 tccctgtaaa gcagcctcat caatttatta aagcttcgtt gaaattcaat aggattcttt
    15001 tcttctaatt taaaatgtat agttaaaatc ctctctggaa gtctttgaga actaatacga
    15061 acacctatgc tcaccgcttc actctcaatc tctaaagaaa ccatttctct accttcaaca
    15121 tacaaagtct ggtacccttc tatataattc tcgataaact tcccatcata attcaatgca
    15181 gacgtcggta tccatgtttt ctgactgtcg tttttaaccg tgtctctgaa ttcatacatt
    15241 ggattatgcc actccatcat atttccctca ctttctagaa caataaatta atatccgcct
    15301 ctttaccttg aatgtttgaa atatcctcaa caaacgcttc aaacacttga tttcctaagc
    15361 gaatattgaa aactgcagga cgtttatttt tgccgtagtt aacactatgc tctatttgtg
    15421 ttgtgaaatt tctatttgca ttttttaaat tcgctgaaag atcaatatca ggtgtacgag
    15481 taaaactatc tgcaatcata ttagccatgt ttcctacatt gctttggaca ctggcaaagc
    15541 cattagttaa acctgcgttt agtccattca tgattgcttg cccagctggg attaacaatt
    15601 ttctatcgta ccgaataggt cctttgtgtt ccctaatcca gtcaccaata cctccaacaa
    15661 aatcttgcac agatttccat gcattttgta acccttctag aaaactatcc atgatagctt
    15721 ttccggctgc tagtaaatcg atatttttca agttatcaaa ccagccagtt actctatcaa
    15781 ccgtatcact aacagcattt actaaattat cccatacctc ttgagcacca tttactaaat
    15841 tgttgaaagt atctatagta ccttgtttta ggttttccca tccctgaatg atgttatcct
    15901 tagttccaat aactagatta ataaaccaag ctttgaaaga attccataaa tctttcgctc
    15961 cttgaatcat attattaaac agatcgattg tcccttgttt taggttattc cacccctgtt
    16021 taatactatt tacaatattg ttagttgtct ctttgatcca tgtagtaaaa gaattccaca
    16081 catctttgat ggtagaagtc aacacattcc agatatttat cacagtatcc tttaaggctg
    16141 tgtaataacc aacaactata tctacaaacg tcgtgataat gttttggata tttgtagtta
    16201 atgtagtcca cagcatcgaa gcatcttctt ttaactgatt aaaattgcct gttatcaaat
    16261 caatcaaaag tagaattgga cccattacag cagtttttat aatttcccat gcagaacctg
    16321 cgattgatcc aatttgagac cataggttcg taaagaaatc aatcattggc tgaaaaacat
    16381 tttttatggc aataacatac ggtgttagaa tgtttacaat tccttcccaa gcggaactgg
    16441 cagcttcttt gatgccatcc catatactcg aaaagaactc ttttgtttca gtccatttat
    16501 tcttgatcca atctgccgct ttcccagggg cttcttgaat tgtagtccaa acattgtctg
    16561 cgccttcttt aatggacttc cataaattgt taaaccattc tcctgtagat ttccatgcat
    16621 tctgaatcca ttctactgcc gagcttacag cagacttgat tccctcccat aagccaatcc
    16681 aaaagtttct aaaatcttca ctcgtattcc aaagatagat gaaacctaca acaagtagtg
    16741 ctaccgcagc tataaccaat ccgactggac tggtaagaaa acctatggcg gaacctaatt
    16801 tcttgaacaa agagattccg ttacctaata cacctaaccc cactttcatg gtttgaaacg
    16861 ctttaaccag cattcctaat gcatacaata ctggcccaat tgcaatagcg attgctccta
    16921 tggccactac taatctttga gttgattctg gagcacttac aaatttttct actaagccgg
    16981 atatggcatc tgctactttt ttgatggatg gtgctagaat cttttgaatt acaatagctg
    17041 ctgactcaaa agctccaaac atttgctcga tggaagaatt catattatct tgcatggtcc
    17101 gagccatatc gtcagctgca ccatcagaat ctttcagaga ttttgttaat ttgcccaatg
    17161 aatcaggtcc tttatcaatc aaagccatca tccctgataa tgattcttgc ccatatagtg
    17221 ttactaaagc attttgttgt tgttcaggcg tcaggccttc aaaagctttt ttaagtaatt
    17281 ctacttgagt ttttaaaggt ttcattttac cgtcagcatc ataaaacgaa acacctaaat
    17341 tatccattgt atcttgcata gcctttgttg gccttgctaa cctagacaat gctcctcgca
    17401 acgttgtacc tgcttgagaa cccttaatac ctgcgtcact cataatacca atagctgctg
    17461 cagtttcttc caaagaaata cccattgaat tggctacagg agcaacatac ttcaatgcct
    17521 ctcccatgtc tccaacttca gcattggtat ccgcagcagc acgagcaaat acatcagcga
    17581 catgtcctgc ttcacttgct tctaaaccaa atcctctcaa agcagtagca gtattttcag
    17641 aagctagagc cacatcccct ccagatacag ctgctaagtc taaaagaccc ggcattgctt
    17701 tcatgatttc ttgtgcgcta aatccagcag aagcaagatt ttccatgccg gcagccgatt
    17761 cttttgcgct aaaagcagtt tttgctccta gatcaatcgc ttgctgtttc atctgttcaa
    17821 atgtgtcgcc tgttgctcct gatatagctt ttacacgact catttgttct tcgaagtcgc
    17881 caccaacttt agcagctgct acgcctactc ctataagagg agtggtaata tactttgtca
    17941 ttgcggcacc agtaccttgc atcactttac ctacagcggt tgtcatacta tttgatttct
    18001 cttcaaaagt cttaacagca tcttgcgcat ctttaaaagt ctttacaaat ccactatctg
    18061 tggcttttaa taaggcttca acagaaaatt gttccatgat tttcctcctt tcctcaagag
    18121 ttagctttag ttagtaagct ttggaatttt ttatcttgtt ttgaaagttc ggaaactccc
    18181 atgattgaat cttcgatttt ttgataattg aagaattctt caaaggatcg atatacagga
    18241 actgtctttt tgcctacttt tttctccgct tggacttgct gatttgccca cgctaattcg
    18301 tgaatcaact tttctttgtc aagccaagat aactgggctg cagtcatacg aatgttgtat
    18361 tcatataacg tcattctttc gatatctgag atattggtca tttccaaata tcgaaaagaa
    18421 ttgataagaa tttgttcgta tgccagtgca gaatctattc cgcttgttgt ttttccgctt
    18481 ctttcaattt ctgattcagg tttcggaccg ctaactttcc cgcgttcgac tccgccaatt
    18541 cttttaggac ttcatcaaac aatttttcga tgtctttaac ttcatcgatg taatcatcca
    18601 tttcatccaa cgtaatagtt tcttcttctg ttctatttgc tatttctaag actcgtgaca
    18661 atgtgttggc attataagaa cgtaattccg gtaagacttt tgctgagagt cccattccga
    18721 attccatatt tccatcgatg aaaggcatca ctttgtctaa ttcacgtaca aatttagtgc
    18781 caaatttaaa cgaatattct ttacctttaa tttttaattt caatgttttt catcctccta
    18841 aaataaaaaa gagagcatct aagccctctt atgctcctgt cgaagttgct ttcacggtat
    18901 ctttgaatgc atattgaaca acatcggctt gatcttctgt caaggttgca taaccatctt
    18961 gaccaacacc atttactgca aatgataaac ttaattcaac gttatcctct gcagcagctg
    19021 atggagtaaa ttcagacaca tatgcttggt aataagtagc tttgtactta tttgcatcat
    19081 catctgtgcc ctgttctgct ttgttgattt cccaaatttc aatgatatcg ccatttaata
    19141 aggcttgttt catttcatct acatgagaat cgcctttggc aactattgaa gtagccgaaa
    19201 aatcatattc aactgggctt aaactttgaa cgtttccgtc ttttgtcact gtagagtctg
    19261 aatctcttga taaaccattt tcgtgttctg tttgaaatgc cattttccaa gcagcttcct
    19321 gagtttcttt tttcaataag cgataaagca aaatgacatc aatacctttt aatgcttcca
    19381 tgttcttcct cctatctaat tctaaattca agtgtgacaa ccgctcgttt taggggcgta
    19441 ttggttgttg tgtcgtccat cacttgaatt ccacttgctt gataatttaa atcccaataa
    19501 tagccttctg tggcttgtat ctgcaacgct tgattaaata gtgccgaagc catatcagac
    19561 acttgtttgc gttttttctg caatccccaa acggaaattg tcaaattgac tgttcctttg
    19621 atgtcggttt tattcggatt gtggactgtt tgggagcttt ccaactcaac aaacggataa
    19681 cctacatcgt ctaatggttt gtagtcgtaa gtttggtatc caagagctag gctcctagca
    19741 aacatttcat caaataatga ttgatctctt gttttaatca tttgaataac ctctgcatgt
    19801 ctttcataaa gattgctttc tgcacattaa atgcgggacc aacgaaaggt tgagcgctca
    19861 tgaatcgagt tccatattct aagtacggac tgtaatcact ctgcggtcca acttgtgcaa
    19921 caaaaccgcc ttccaacaac cacattcgaa ttgatcgtct agtgaatcct gtcgggtgca
    19981 caaacacatc cccttcccaa tgtccattaa agcgggcttt ttcttgcatg cgcttagtaa
    20041 gctcggctcc atttgtttta actaccattt gcacatctct aagcgtgaca gcttcttcca
    20101 agtgtttaag cagctcatcc acacctttgt aacccaatga aactttcact ttgcaatcac
    20161 ctcctggacg ataatgctat tcgttgataa caggctgcgt tgagcgacat ctttgtatgt
    20221 ctttccatta aaaataatgc ggtcatattt tggaacaaca aaaagaggca tggttctgat
    20281 aaccttcgcc ccttgtcgta tatccccaaa aatcgccaca cttctatctg ttcctaagtc
    20341 cgttacattt gcgtttgttg ttaccgtaat cggtgcttct tctatccact cgcctaattc
    20401 tggatcgtaa cgatttcctt ctccatcttt ttcaaagata acctcatcta aaaatctcat
    20461 aagaaccgca cgctcccttt tctaggtttg tacaattctt cgtcatcttt ccgtttgaaa
    20521 tcatcaattt ccgatgcgta ttctgaaaaa tcggaatcag ggaaagccat cgataaaccc
    20581 tcttgagcgt atgaagtcat gccctcttgc ccaattcggt taaaacgttt gttcgttacg
    20641 tcaaaaacga tgtattctag tgattctggt gtaacagaaa cgcctagcaa tgacgctaag
    20701 cgctctctag ttcgtctctc gataacttct agcttatcat ctgtagaacc gcttagaagc
    20761 ttcttaacgt catctttaat taccattcgt acctatcctt cctacggctc agtcagtgtc
    20821 aaaacgtgag tatctgtgtg tgatccatct tccgttttta ttgttgtcgt gtatttaccg
    20881 gctggtactg tgtcagtcca agtgatatta ccgctagaag aaaccgtcaa tccgcttgtt
    20941 actggcgcaa ttgaatacgt cactgtttta tttgttgcgt tttgtggtgc aacggtagct
    21001 gttaattgtc tattgccagc agtccccgca actgcgcttg aggttttagg cgttacagta
    21061 acgcctataa cggggattgt tactcccccg ccggttcaat tttaccgaac gcagcatctt
    21121 tgacgatcat aaagccgaca tccattgttg cacgcaacgc aaccagctct tgttcaaaca
    21181 agtttacagg agtgccatct tcgtttttaa gcgtagataa ttgcgcatct tctgaaatct
    21241 tgaacgaaat gttgaatggg ataccataga acatatagtt aaagtcacca gcgtacaatt
    21301 cacctttacc caatgctttc aagtcaacca caggaagacc atcaatcgta ttcgccgagc
    21361 ggtcatagat aaattcaacg tttgtaccga ctgtttgagc ggcagaacgc aattctgtgc
    21421 ggtttttacg atttgagatg aacgcatttg gttcaaattc atttttgcct aaagcatctt
    21481 ctaatgccaa aatgttgtca taagtcaaag gacctttgat aacattagcc gctgcaacga
    21541 cagattcttc taaagactgt ggaaacggat tgtcagtgtt tagtaatgca gccgcatcaa
    21601 actttttgta gaaagcttca gcaattttag gttgcataac agtgaagaaa tctgacattt
    21661 tgtagtgcaa gtattcacga gacacaggaa taatcacgcc tagcttttta gctaccattg
    21721 tcgcttgcaa ccatttaggt ttagatgttt gaatcttctc accttcacca acccagtatg
    21781 cgccaggacc ttcagcaaag tattcaaatt tcttttcctt atctgtcatt tcttcgtatt
    21841 tcgccaattg cattactttc gagttttcca taatgtcgtt gataatcaaa gtattatact
    21901 tgtcagggat tttaccctct ttagtttcat aaactgttac gttatctgga ttccatgttt
    21961 gtgccatatt gtttattcct cctatttgat aattcgattt ttagcagcta gtttagccac
    22021 tgtttcttta gtatttttcg atgctgtaaa ttgtccacct tcatttggtg gtgtttgtct
    22081 tgcgttttct ctcttaatca aagaagcaaa gttagtgatg actgctacag cttgttttgt
    22141 ggcatctgca tcatcagaaa caatcagacc aagtaaatca tcatcgtgtg gtaaatttgc
    22201 atctgtcagc attttagaag cttctttcgt catttcagat agtgcctgtc cacgctttaa
    22261 ttcagcgatt tcagcttctt tttgttccaa ctcatgctgt agtttttctt ccgcattcat
    22321 ttttgccagc tttttagctt cttctttttt tgcttctagt tctttttccc acgctgcttt
    22381 tgctttattc gtctcagcag cgatcatttt cgctacttca tcacgagaaa atgttttgcc
    22441 agtattgttt tcttcttttg cttcggtcgg tgtctcttgt gagccagctg gtaggtttcc
    22501 ttgttgtccc tcatcaccag atccaccatc tcctggttca gaaaaaaatt gtaagttcat
    22561 tggcataaat aaacgttttt tcatgattaa tcctccacgg ttacgccgct acccgataaa
    22621 tttgaccagt tacgccggtc agtcggaaca gctcaattta tattgccccg agcagtagag
    22681 ggcataataa aaagcccgtt attcggcttc acttacatct cctgaaggta aatatttcac
    22741 gctgatatca atcgattgaa taccgtcttc ccattcacta tcagtttcaa cggatactct
    22801 tagcactttt tcgttttgta gaatcttatt taaacttgcg atagctctaa caattgcttc
    22861 ttcattttcc atatgcgtac tctcctctac atagaagcgc tttggaacac gctttgattc
    22921 atattgtttt gcaaagcttt gaagtactct gggttagact gaaccaattc ggttattgat
    22981 tgcacgacag gttcaataac ctttctagcc gtttcataca cttcttttac gattggttct
    23041 aagacatcaa ttaaatgctg tttaacggca tcatagtctt ctttaaattg agcatcattt
    23101 tgatacaaat gcaaaatttc ctctttgctt ttcccatatg ccgtttctaa aaatctgtca
    23161 gatataactt gaaatgaatc tttcttcatt gttataacct tctttcttta agtgacttct
    23221 caaaagcatc acgatcaaca atcggataag tagaacatct atcaaaagga tgtactggca
    23281 tcatattctt acctacttcc atttcactca ctaaatatgg accgtcagcg gcaatgctct
    23341 tacataatcg gcaagcatta ggttcagtta cccatccata tttctcaata ttcgcatcta
    23401 agtaactttc cctctgtata ccgctttgaa ctcttgttgt ttcagttacc attaaacgtt
    23461 gcgtattaaa cttagtattt tctcgtcctt cttctgttag aaacttactt aattctggag
    23521 cgagctgttt aggattacgt cccatcgtca cacttcgaac aagcaactta tccaaatctg
    23581 ctttcaactc tgcttggtac atccatagcc tttcgctgaa cgttgcaaat ccatccgctc
    23641 gaaacgagct gtttatcact tgctccacta attttgcata accgctttta gcaatcgcca
    23701 tttctaagat gccagcttgc cgcttaagct cgtctagccc tgcttttgta agttcattcg
    23761 agaaatactt atctagctcg ttaaacgtag ctataagctc taaaccaata ttggctttta
    23821 acaattccag tcgattgaca cgcatcgtta gattgtatag cttcaattct ttgttcgctg
    23881 taggtgagaa gtctttctct ttaacatact tcttagcctt tctagcaaat gcttttacat
    23941 ccatttcatt agcacgcttc attgcttcgc tacgagtaat tttctgcccg tttgaaaaac
    24001 tattccattg tgcgtctatc tctttctgta tcgcatcctg cgcgtattgc agacgcttct
    24061 tgatctcatt catgcgtttc ttgtcatctt taatctgttg ctcttgccaa gctttctccc
    24121 ttttgatgaa gtaatcttgt gatttcactt aatcactcct cgatgatttc gtaggtctca
    24181 ataaagatat ctggtttgca cggataaaat tcaccatgaa cgcctttgat gatgtaatca
    24241 ccatcagata tttccattgt gccttctaat gtttcaatac caggtaatcc attgggcata
    24301 ttattatggt caatccatgc ttcaccaacg aattctttgc atttgcgata tgaacgtgca
    24361 gatagcattt taagttgcac aatctcgaca gtaactggtt ttttacgtgc tttcatcttc
    24421 ttcaacctcc gtatcagttt ctttgtcact gtcaaacaca cctctgtccg ctgacgtttc
    24481 agcattcaca cgttttaatt ctgcctgtac atctggaaca aaagaagcga gtcctaagat
    24541 cgtctcttga ctgagttcag ctccagcatc aaccaaagat ttcaactctt ccagaatggc
    24601 tttaggtaga ttaggcgtaa atattacacg taagcctttc aaatcggagt tatccatttc
    24661 agaaatactt gattttaggc taaacaaaag acgataacgc cgcataagac cctttttgaa
    24721 tagcctttgc tttgttgccg tcatttgttc aaatccaaat aatttatatt tcatcgcttc
    24781 tcctgattgc actccggaaa aattgtcatc agtaagatca ggaaccattg agatttcgtg
    24841 gatatccttg cgtactctgt ctttgtatgc ttctacaccg ttcacatcat attgtttgta
    24901 aatatatcct gcagtcacac ttgttttatt accgttcaca tcagttccag attcaagcaa
    24961 aagcatattc gcttctttct gcttgatggc gtcctctgtg gatagtcctg ctgcttcaat
    25021 atcaccacta ataactagaa gagcatcgtt tagatcagtc atatagttgg cggtatcaga
    25081 ctgccctgca tcgtatagat caatcaaaga tagtacatct tcatacaagc ccatccgaaa
    25141 acgattagga gaatactctg taataggtac ctctttatat tcatgcggtt catcctgggg
    25201 attttttaac tcaattgctg ttagtgtcgt ctcatcataa gtgatacttt tttcttttgt
    25261 gtatacgatt ggttgaatgt actgtttatc agcatccttg ttgaatcttg tcttaggata
    25321 ccgtacagcc aaaataggct ctcgttttac tgtagtatca tatacaacaa atgtttcaaa
    25381 tacattagcc aaatcaacat aatctgtatc atctgaatct cgatagataa tctcataggc
    25441 tctcccatac ttatccatat caagccagag ttcagcattt aacccatcta tgtcattatt
    25501 agtattaaac tcttcgattt ctttttgttg atttgtatcc tcgatttgca cttttatggg
    25561 attgcctgtg ttgtacccaa catcaaacgt acaaagaact tttccaaagt tatgtgctga
    25621 tcggtgatcc gctttttcct tttctctacg tctacggtta tccatgatat ttgtatttct
    25681 agctttgtaa taatcatcta atacactaag ccgctttacc tgatattcat gatgatgttt
    25741 tatcattgct gctaaagtat ttgaatcgtt aagtaaatct tctgctgagc taaatctata
    25801 gtgaagattt gattctacgc taaactttac ataatttgtg ttcacgtcgt tcgaataatg
    25861 tatatcagta ccgtattcaa attcgtttac cttatccatt tctcacactc cttaaaacat
    25921 acgtttaaca cgtttaatct tttcgttgac attaactttt ggtttcttaa gaagctcatg
    25981 agtatatatc gcatatcgaa cggagtctag cacatcatca tattctttta ttggctcacc
    26041 ttttttcttg tcccaaatgt attgataaat ttcatcccga aatttcatca ctctatcttg
    26101 acagataaac aatttatctt gtttaaatct cttggcgact tcctcaactc cagataaccg
    26161 tgctttatga gcatttttag cattaatctt ttctcttaca aacctagcca catgctccgg
    26221 tctcgcagag tcacaataaa aaggcactcg taagccgtaa cgctcttgaa tgccttttgc
    26281 tatatctacc caataatcaa tctcttcaaa ttgagtagca tgttcttcga ttaaataagc
    26341 tgttccgtca tccgtttcac ctataactac gattgaaccc cagtgttcat atccccagtc
    26401 aacaccacaa taaaagttgc tcaaaggtgg taagtctttt gactggatgt agtgcttgct
    26461 agcatcaaaa tcacgataaa cgacaccttc tgcagaaacc cataaacctt tgatatcacg
    26521 atcataaaac atgccgcttg gtgttgattc tttaatatta tttctatagc gttcagataa
    26581 aaaagtatta tcatccaatt caaagtggaa cgattgaatg tttttacttg aattgtcgat
    26641 atactctttc ttcagccagt gttccgggtt gtcagggttt gtgtcagcta ggattctcgc
    26701 tcctgttcct gaacaacgtg aaacgatttc agcaaatact tcttgtctag ctagtgatgc
    26761 ctcattgata taagcgccat atgctgtcat accacgaatt gcaccaacac cgccaatgtt
    26821 tcctgtataa gcttgtacga ccttaacgcc aaataactta aagtttccat gtttatcgaa
    26881 tttaggttct atgctgtaca tgttgtatag ttcctgtaag atgttctttt ggattgttgc
    26941 acttgaaact cctgctagga tatacattgg ttccttaatg tcttcttcat cggcaatctt
    27001 tcgcacacgt cgcaattcaa acaagaacaa atcattgttt atttttgttt tcccagaacg
    27061 ttttgctcca tgtaataaag taataaacca atcattctta atggttttat ttaacacgtc
    27121 gatttgcttt gggttataaa tatcaactag tcccatctaa ttcaccacta atcttttcta
    27181 acaactcatc aagtttttct tcggtagaac gctctgagtt tatctgtaat tgttctaatt
    27241 ttgcttgcat taattttatt tctgtttgag ctttttctaa ttgcgtttgt gataacagcg
    27301 atttatactt caaatacaaa tccaaggctt tcaatttgct ttctaaatct ggtgtatatt
    27361 catattgcat atgtttaata accttgtttt tctccaagcg gtctatttgc ttgctcacgc
    27421 ttatttgtac ttctccttgc caaatatcta tcaattcatt taaagcgtcc tcagcactta
    27481 atttgcgctt cttttcgata ggatttagcc gttcattaat atactgtatt atgttaggtt
    27541 tagttaggtt ttccttacct attactcttg ctgatcgttt gctataacct gctttaatgg
    27601 cggcttgagt agcattacca ccatttttta tatattcatc cgcaaaagct ttttgttttg
    27661 gtgtcaaatt taccacattc aagccaccac ctttcaattt tattaacaat atctctcaat
    27721 atttgtctgt atgttctgct cactaaaata tccatggcca cagtaacgaa gattgtactt
    27781 atcgatctct ttcggtgtag cttctctggt catttcaatg atggagtact tctttttgat
    27841 ttggactgat tggacaactc taattggatc atctgcgttt ggttgcggat atttatttga
    27901 taatgataca taccagtagt ttctcattat gtatcactct ctctgttggt tattggcaga
    27961 aaaggttcgc atcataaatt ccatagcctg tccttcatta aacccttgta gaataagctg
    28021 atcgtagaaa tacttagctt gttttgcgat taacgctaag cttttttgag tttcatagaa
    28081 tgtcgcctcc atggtttggt ttgcttcatt cgttttaaat aactctctta attggttttc
    28141 attcatagat aaaactcctt tcaaaataaa aagaccactc aatgagtgat ctaatatgta
    28201 atacaggacc cactatcctt cgagggaatg tggattacag aaacatcagc gcgctatagt
    28261 ttccaacctg tatattcggt tttactgaca ggcagttatg gatcaccgta aaccaaagtc
    28321 actggcaatg aatcgaacat tgcaacacca ttttccgata ttttacagac tctccatcat
    28381 aaaaactctc acctctagaa ttgtctatct ttaggggatt tatttcaaga tgcctcttgt
    28441 cctttaccct cgacggtctc ttgcctagct gcataaacta agctcaggtg ctcattacca
    28501 ctgatttaag ttattagcgt tacctttccg ccacagtgac aaatttatat tgtgaaaata
    28561 aatactaagc gtataatttt aattatcagc gagtggtccg ctgaaataaa ttataggtgg
    28621 tgaaaaattt gtactttgtt ataagagaag caactaatgg ccaatattac ttcgttatta
    28681 agtccaataa caatgaagta gtagcaacta gcgaaacata cttgactaaa tattccgctg
    28741 aacaaaccat taattctata aaaaacggaa tcaccaaaga ctctcaagtt atcgatatga
    28801 ctaaataaga cgactaactt catttgctag ggagttcatt tcagctgctt tttctaccaa
    28861 agcagttgag atactttcta gcttttctga tttaacagaa ttaaatactg gcgtcttgat
    28921 actaatcgat acatcttgac gaggatcagg actcgttgct ctattttttt gcaccactga
    28981 tctgtacttt tctactgaat gatactcaat tacttgttct aaatgttttt tgtactcttt
    29041 acatacagct tctttttgga tcaattctaa acgttgttgc tcaataatat caatcaaccg
    29101 ttttcgatcc ataccttgat actttatttg ttcgacttgg tcactagcct tgttgagcag
    29161 ttcttttagt tcagtcaaat ctaatttgac agttgctttg atttctttat ccattcgttt
    29221 tccctccaat acataaatta atagacagca acggatgata gataataaga acaatcagaa
    29281 ggagttgaaa ttcacatcct tattcttaat atttccgttg ctgtctatcg aagcttaatt
    29341 gtgaaacaat aataaaacga tgttcctttt attattattt tgtctcagac ctatcactaa
    29401 tctttcgaca ctaccataat atcacgttaa aacactcaaa aaccctacac tatccctaca
    29461 aaaaccctac aaaatcaacg atactgaact aatacgcctt ttttgtatgc ttcagcaaat
    29521 tcgatcaatg cgatggattt cagcttctct acattcttct ctccgtatcc tcgtatcaat
    29581 tgacctattt cataattaga gtgcttattt acgtcacaga agctgtagta gagtatctga
    29641 cgactaatca gactgagagc catcaaagcc gctaaaatcg cgtctctctc tgcttctata
    29701 tccatcattt gaatgatcgc gtcttctgcc ttattgacgt gctttggtgc cttcggcata
    29761 tcggttatga taggcgactt aatgtctatc aaagagcgac ctgccatccg ctccaagcgc
    29821 cgaaagttct tcagcacatc tctcgcatta catcttgtct gtttgaaatc tacctctcgt
    29881 aacaattgca tcaagtcaaa ccgctccttt atgtgatata ataaacttgt ggaatttatt
    29941 agatcagtcg gagcgatccg gcttttttat ttgtcattga ttagtttaat atccaccaat
    30001 ctcgctacag ctaaattctc tttgcttttc gctaaccact tgtcgcattc catcgtgttt
    30061 tcaatacgaa tgatcgctga gtgattataa acatgttcta catatccacg aaatggatag
    30121 atgaatcctt ctgcttcaca tcgaaccatg tcgccgactt tgaattttgg tttcttacgt
    30181 gttttagggt tctttgtcgg catatctagc attaaaccgc cgatgccgtg gctgctggag
    30241 taaaatccgt cttttagttt cattccgctt cctcctgttt taattttcac catttctttg
    30301 ttgaaaaccc gcaacgtcta tactgtttaa ttcgtttctt aggtctttct agatcaacca
    30361 gaataaatct gtcggatata tgataatctt ctattacagc cgtatacatt ggattcgaaa
    30421 ccgagtacca tgatcttttt ttatcaaact catccgcatc atctcttgta atttctatca
    30481 tgttagcaga cattagatag ctctcttctt ctgcctcgaa tgggcaggaa tattcatccc
    30541 agcttcgagg aaacctggcc aaatctcttt ttaaataaac ttgaacattc attccgcttc
    30601 ctcctcatca tagaaaatca ttccacccaa gacctacgct atgcataaag caaaattcgt
    30661 tacacaatag atgtctttta tggtattcat tcttaaaact caatctgttt ccacattctg
    30721 aacaataaag tttcactttc ttatctggat aaccgttttt gattaactgc tttttaaatt
    30781 gtttattttt ttggcgttta ttcattccgc ttcctcctgt tttaatcccc attgagcgaa
    30841 tgctgctaga acttcaaatt cttctttcat agtaaaatca tcataaaaac caaaaatatt
    30901 cgtcggcata tcgtttgctg catgctttaa cacataaaaa ggaaacggcc attgtgattt
    30961 tgcgtaatat tcttctaacc actccaacac gattttctgg ttgtcgttga gcgcttgtcc
    31021 atgagtgaga actttacccg attcccaatc tcctgttttt ctagcatatt catattgttc
    31081 tgctgttaag taatcgctca tctttctacc acctcttcca ctggcacagc aaatggccag
    31141 tatctttcat caatttcttt gatttccgct tctgttagct gataagcaga tttttcccaa
    31201 gtacatagcg agcgactaat atcaaaacag aaatcatttc tgttattaaa tttcttgata
    31261 agatataagt caccaataat aacttcatac aacggttctt tctcaacctc gtagccgttg
    31321 tataggctca atagtgtttc atatgattgt ctcgttgccc aagcgagtaa ttcttctcct
    31381 tgttcttgcg tcaatatgcc atctcgcaca aaccaatcac agaaataata tgaatcacca
    31441 tctttggatt tgattaaata cgctattttt tcagcttttg ttaaattgtc ggtacttttt
    31501 tcgatccaac ctgccacgag ttgtggaata actggtttct gcgattcatt tagttgttgg
    31561 attagttcta atgcgtgttc taaacccgca tattttccag catgaaaagt acttatacct
    31621 ttaaagtcct ctatgctacc tacaaggctt gatagtttat caatcaattc ctgtttattc
    31681 atcgctgttc ctccaataac tcgctattct cgtatatatt cccgatgact tccaaaggtg
    31741 aatctacaaa gtacaaaaaa tcaaattccg tgccgagatc tttgtaatca acacaaaatg
    31801 cgccttcttc aaactttatc gttgaaaaag ttactttcaa atcagcatcg agagttttga
    31861 caatatcccc ttcaaatata tccacaccat tcttatcttt cattcctgtg gattgcatga
    31921 gttcaatatc aagaaaatca ataatttctt caaactgctt atcttcttct aaatcttccc
    31981 tcaaaactaa acaattgcca taaataaaat taggtaatgc ctcagcaatc accccattaa
    32041 gatgattgct taaaatatca ggttttaccc acgctctaaa cttcggtatc attcgctgtc
    32101 ctcctcgtat ttttcaatca attccattac ttttttcact atttcaaact caaccgcttt
    32161 tgattcttcg aaatcatgaa caatttctgg aaacagtaca tcatcaacta cccacaaaat
    32221 aatctggcgc tttccaccaa aatttatgat tagatggtcc gattccacag atagtgttgc
    32281 tcctgattcg atatcatata aatccatgct gaattgaacg agttttttta tcatttgctg
    32341 tcctcctcaa atactcttct aatatttctt tatacttctc tacaaatttg aaacgatctt
    32401 gatgaagttt cttgctccaa tttgtttgcc gatccagctc acgcatctga tcgaaccctt
    32461 tttgaatttc gttgtaataa aattcaatgt ttgctgctgc tttccaatgc ctgctacttc
    32521 gcactcctgc tcctgtttca gccatttcta acttaactaa ttcagctcgt tcttttgatt
    32581 ttttgtcttt ctgaatcttc atcatgattt tcttgaggat gatgtcactg tattgtgtaa
    32641 tgagatccat tatctctcct ccacatacct aaactgtcgt ccttttgaat caatccataa
    32701 gctcctagct ctatcccaga taatgttttt gcttaatcca gtaatttcag ataactgttc
    32761 agctgtacct gttactagaa ttcgatcacc atgccagatt gcaatttttc tcggcgtttt
    32821 ccgtttaggc ttttcagtcc acattgattt accgagcttt tggacttctg caactatttc
    32881 tttgtcttcc tgccaagatt ctgacttggt taattcagca attcgtttca ttgtcgcttt
    32941 cttatccacg ctcattcctc caatctacga atttcccttc ttaagttctc tatgtgcaaa
    33001 tcgattgcct tcctcgccgt ttcattgacc atcactgcct ttgttcgttc cagatcgtca
    33061 atctcacgct gaaggcttcg aatacgcatt tgaatcactt cttctgttgt catgatggac
    33121 cacctcgcta aaaacgctct tccttgaacg tattccgata ttttttagct aatatcaacg
    33181 gcgcttgata ttgatgacag aacaactttg ccttgatctt aaagtctttt gtctgcattc
    33241 ctttgacatc tacgactttg acaagtttgc cgtttttata aaatgtgaag tcgggaatat
    33301 actcgatctt gcgatacttc tttccgtcta gttcaaattt cggcatcagc tcaaatcttt
    33361 cctgaagttt tactttccag ccatttgctt cagcttgcca caaggctaga tcgtaatact
    33421 ctgcttctgc gatagaatca aacttgatac ctcgatgaac agttttttta ttacggtatt
    33481 tattcatgcg atactacctt tcactggttt tatgcgcttg tctgctgttt gttggaattt
    33541 cagcgcataa ccttctgaat tcttaaatat cctagaaaca attctttcac cgtaggcttc
    33601 tcttagttca ggaccagata agtttgttgt gatgatcgtt gccttgttct gtctggcttc
    33661 taagagcgtg tttaacgtgt tgtttgtaaa ctgcctacta tttgataccc cactacctaa
    33721 ttcagctcca atatcgtcaa aaaccaccaa atcagttgtt ttaatatcgg ctataagcga
    33781 tccttcaatt tcttttctca gttcagtatt gttataagaa aactttattt gctctaataa
    33841 ttcttgatag cttataaaaa gtattttctt gtcataattt gagcgctcaa gtatttccca
    33901 agctgtggcc attgataagt ggctttttcc gcttcctgat ttccctgata gaatgaaatg
    33961 tgcaggatgg ttcagcagaa cctcattcgt atagcttttt gctttttcta aagcgatttt
    34021 tgtttcttga tccactacgt gataattttc cattttgcat ttaaacaaag ttttatctgt
    34081 taataccgaa ccattttgaa aaaaactcaa cgctcgtgct tttaagctat cgttatatat
    34141 ccgttcggtc tgtatatcct ctttcacacg taacgcttta taaccacaac tcatgcatgt
    34201 tggtttacaa cgttctgaac catccttatt tttagctcgc caactataca aaggttcgct
    34261 acattctgga cattttccgc tttgcactaa tactcttctt attagtttct ccatagcatt
    34321 tgctaggctt tccatgtgat gcatctcctt tttaaattgg caagtcgtca tattcactag
    34381 gattgctgta ctgtagtttt tgactttgct ttttatgatt cttcttgtct gctttgattt
    34441 cgaatttgag cttctcaaat ttttctctca atttcttagc acttctaata tttccaaacc
    34501 aaaattcatt tgtaggtagc caattgatca catactcaat cgcttctata gatgctttat
    34561 ctctttcttc catcaacctg attgtgtctg cccatttttc gatatctact ttgttcattt
    34621 cttttggaaa atcttcagtt aaattacttt gcaacttttt agcaaggcgt aagtgttcgt
    34681 cagaatactt acctttcttt tcttctttat ctatatcttt atcttcttct atatctttat
    34741 ctgtaccgtc acgtgacgtc acgctaacgt cattttccaa tttgagacgt tcctgtctct
    34801 ttctttccct gtatttacgg tttctttcag cattttttag ccttacttta tccataccct
    34861 cgatattttg atgtttttcc caattactga tggcaattag tccatcactg cttagatcaa
    34921 tcatgttgaa atttgccaat gtagttagcg ctaagcgaac cgtatttacg tttttgccaa
    34981 acaatgtagc aagcatttct tcggtataag gcatgttcct ctggatatat atcagaccat
    35041 cgtcgttagt ctttcctgct aaaactagta atcgaatcca tataacgatg atggcatccg
    35101 actcaggaac agcttggatt aatcgtattt tttcatcgtc aaacatagta gttttaagtt
    35161 tgatccaact tatctcagcc aaatttatcc tcctatcctt aactttttaa ttgtttcctg
    35221 gtttaacttg atccctttga tttgatattt atttttgaaa ttgatcacac ctattttgtg
    35281 tttctctgtg tgatggattc tgcagagtgc tgcaaatgtg tactctgaat gatcaacttc
    35341 tttgcgcttt cgtcttccca gcgctttgtc aaagtgatcg atatcagctc ctgttttgcc
    35401 acagatgcag cagactcttt ttgtaatgca tttgtagaag taatactctt gattcgctgg
    35461 taaaatctca tatccttctt tgaaaggaat atgatgttca aagatgaaat ctaggatgat
    35521 attcgctaag atattggcat cactcacggt tgtattcgat tcgtctttca ggcttatttt
    35581 gcgccctgtg acaccttcaa aacgaaagta gaagaattcc ttccagaagt ccgttggcat
    35641 gcctgtatcg ataaaaatat cgcctatcag cgcatagatg aagtttcgtt gctgtacggt
    35701 gaaacgtcta ggatcaataa aacgaatttc aatgactcga tcaccatcgt agccgtcata
    35761 catcgtcttt agtcgatcaa tgttcacttc ctcattgatg gttgcgctta tgtcttttcc
    35821 tttgaacttt ttcagaaccg ctgaatatga atcgattaat ggtttaaaca ctcatatcac
    35881 ttcttatcta attcttttct cttagctgct attgctcgct ccatcaaggc acattgctca
    35941 tagcttaact gttcaatagt ttcaacgtta tcagctaaga gccctaattt atctgtctgc
    36001 tcattaacat attcgattaa ggttttggtc atatctttac ccatctgctc attgaaagct
    36061 tctagaatcg tctctagcat gtttaatttc tttgtatcga ttctaggtgg tgttggaata
    36121 tcttcccctt gaaatacata taatcccagt ccgtgtagag ccaatgcttt cacaaagcat
    36181 cgcttcaatg agttattgat ttgcattgca tttggtttaa caactggttg gtttcgataa
    36241 tctaaaacag gaaataattc ggtttccgtg tgtcctttaa ccgttactga gacagataca
    36301 taagtcccag tttcatccat aagaaaaggt ttatattcct caacaagaaa gtcttgatga
    36361 gttccagaaa caaccctgta gtgtttgtac tcattaatag ttaccgttgc ctgtggatca
    36421 ttctttttca taatctccca cgcgtgagcc caagataaat aatcaaaatc tccttttttc
    36481 ttgagaattt tatttaactt acgactaaaa agtttttcaa agttcgttgt ccctttgatt
    36541 tcactcatca aattctgcct ccatttcagc aatgtatttc ttacctggtc cgtaataaga
    36601 gatatcaatc aagttatctc tgtcgtactc ttctagcgca tcaatcaagc catcttcgat
    36661 gacatagata tactcaggtt ttttggactt cctcgataaa tggataagat agacatgatc
    36721 ccaaatactc acaaaatttc ccaagtcatc ttgatcacat gctagttctt catccgtcaa
    36781 gagatttcgt ctgatttttc gattatttgt ttccttgata ttcgatttgc cccaactagg
    36841 atcagtcaaa tattgatcta gagtggaaag ttcattttcc atatgttaaa atctccttag
    36901 ttatgatttt ttgagtgact cattgctttg gtcggcggag tcactttttt atttgttgcc
    36961 atgctttttg cttttcgata tgttgcttgc ttaaaataat aggacggcta tttgcccacc
    37021 aattatcagc aatcacttta ccgattttta gcgcttcttc tcgtgccata gttgctcctt
    37081 tcttttgaat caagcagatt gattaaaacc atcaatgctg cgaacaaact tcccccgata
    37141 atactttggt gtactactat cactaatagc cctaggatga atcctataaa aagtgtgtct
    37201 gtcttcttca taatctaatc tccctatttt ttatttctag cattctcaaa tcctcaagtt
    37261 cagaagcgat tagttcagct tgtctatctg atagctcatc ggcttttcta agcgcttcac
    37321 gatcatcttg taattgtttc ctgcgttgtt taatcaaacg gagaatttga tgttcttgtt
    37381 gcaatgtgta ggacataaaa tcattctcct ttgcctttag aactcaaagt tttctttcaa
    37441 aaatctttgg agttccgatc gttcaattct gatgtctaac ttgctccact gctgtgtttt
    37501 taagcctagg tttatccaat gtgttaattt gtcatcacca attcctaaaa cttttcttac
    37561 ctctgatttg tttggatatg gaggaagctc tactgatttg ttcataaggt gcaatcgttc
    37621 gtccaatgaa ttaagcacag cattcgtaat ttgtgtagtt aattctgaaa ctactaaatt
    37681 atctggaatt gttatttgca tgctttttct ccttttctaa ttctgctagt actgcctcaa
    37741 ttggcttgat ttgtttatct ggttttctac gcccgttcat aatatccgac atataggctg
    37801 ttgaaatatc aagcttttca gctaaccaag cttgactctt gttatgcgta gctagcgcca
    37861 cacgcacttt taaaatgaag tcctgcgaca taactatctc tcctctaata gatcaatttc
    37921 tggaatatat ccttcttttt ttagcgactc ataaatgaac aaacgtcctt tttgagtcca
    37981 ttttgtattc atcacaactt ttgttccacc atcagatttc ggaatctcag ttgtatgaga
    38041 ttttgtatat ccttgtctca tatgtttctt gcataataac cattggttgc ctactttttt
    38101 ctgaatacct agtttatgaa gtagtttgtt catctgttgt ggagacatcc cataatctgc
    38161 tgcaatctga ctaattgtta ctgaatctgt agaagataag atactatcca aatacgaaat
    38221 tttgggttcg tattctgcaa ttttttgttc tgcgattagt cttccagtac gttcttcttt
    38281 caacttagtt gctaattgga tgattgtatc tggattaagc aaggcttctt ctactttttc
    38341 tggagttaga taacctccat gttttctaat tgctggcaac acttcacttg ttacccatcg
    38401 tttgaatttt ttggcagaag gaagttttga ttttaagatt aaactgtaaa ggcctgactc
    38461 gttgatgatt gtcatattac gattttgacc tgatgcacta attcggtgca ttagcctatc
    38521 ctcttcgtca acatgatttc tgacagcgtt gtctgcacgt tcgtatccaa gaatctcagc
    38581 tacatcttta cctacaaaat acggttcatc atttaataaa actgttctta cttcctgttg
    38641 tccgaaatta aaaatttgtg gtgtgttcat tttgctcatt cctttctttg gtataatttt
    38701 gaatagaaag cgaggtgaaa atagtatgga agaatttaat atggatgtcg acgccttatt
    38761 caaacaaacc gtattcaaga ctgttaataa agaattcaaa atagatttcc aaaatgacga
    38821 atcttttcca acagaaattg aattattcga agaactttct cagagcgttt ctgagtcttt
    38881 ttctcgccag ttgcaaaaga atttttttga tgctctcgtg gatgaatttc atcaacaaca
    38941 gcactaaact tttggtcttt aaaatttaaa gttattaaag ctccttttgc ggaaggagtt
    39001 tttttatttt gtttcatgat gtttcctcct tttctttaaa tatgtaagct aacaaaatta
    39061 gctaattttg ttgacagttt ctacaaaatt ttgtagaata agtgcatagt taaataagca
    39121 cagaattacc ctataaatta acattctaag tttcccgacc ttgaatttgt ttactttatt
    39181 aggtatcttt cttattgctt gttagcttat taaattagct tacgaacata tattactata
    39241 aagttttgta gatgtcaacg attatctaca aagtttttaa gatgtgtttt aggcatgatc
    39301 ggagaatcat tattatgaca acatttgaga gagtaaaaat gttagcagat aaacgcaaaa
    39361 tatctattgt cgaattggaa gaaaaactta attttagtaa aaattcactt tatgcgtgga
    39421 aaaagagtaa gccatccatt gataaactaa atgcagttgc tgactatttc catgtttcaa
    39481 cagattattt gctaggacgc acagatgatc ctaacgcggg agttgcacca gaggaaagaa
    39541 aactaaccgt ggaagaagct ttagcatctg ttatgagtag cgacggaaaa ccgctcaccg
    39601 ataatgatag ggaaatacta tcagctatga ttgaagcata tttagagaaa aaagataact
    39661 aaataagtag gtgagtcatt tggacagtca aattgaaatg ataattaatg aactcggcgt
    39721 taaggtagaa gagcgtgaaa accttgatgc cgatggccat tatgttgctt gtatgaatac
    39781 catagtaata aaagctaatt tatctaagta tagaagacaa agaaccttat tacatgaatt
    39841 aggacacgct tctaaacatc atgataatta ttttttatat aacttagcat tctctctcca
    39901 ttcaaaaatg gaatatgagg ctgatcgctt catgattgaa aaattattag atagatatat
    39961 tgcaaagtct gaattagaac cacacaatat caattacatg aaatttatag aagataacaa
    40021 tttaagcgtt cgcttcgaac cacttgtgaa agaattatta aaagctcgca tctattgtta
    40081 tgcagctctc taaaattttt taagcaaaaa aagaacatat gttcaaaaat agaaaggtga
    40141 acaaaaatga tatatacaga attcaaagaa tggttagaaa aaaacacaac cggatacgaa
    40201 acatttatcg tcaaagctac taattatcaa attgaaaaaa acaaaaatag acccccaaaa
    40261 aaacgctggg atgataagaa aatagataaa gctgtattag aaatgtggaa acaagtcgtg
    40321 actaacttgt atcaaacaat tcgtaaagaa aaaggagttc cattaattaa cgggaaggaa
    40381 atatggcttg aatttataga ggaacaagga ctgatcgaat ttttcaatga tagcatggca
    40441 gaattagaat ttgaataggg gtaatattga tggcaatgat aaaacaatat aaaaagaaaa
    40501 atggcgaaaa agcatggtac tttaaaactt atctcggtat tgatccgcta actggaaaga
    40561 aaaaatatac tactaaacga ggatttagaa cacaaaaaga agcaaaaaca gcactttcta
    40621 ggttagaact agaattacaa aaaacaggaa tgcccacaag tacaaatact actttcaaag
    40681 aagcagcaga attatggcta gaaagctaca aaaaaactgt aaaagaaagt tcatattcaa
    40741 ggactaaaat aatctttaat aaacatatat atcccaaatt tggaaatatt aagctttcta
    40801 aaattaatac ggcatattgt caaaaggtag taaatgattg gagtgaaaaa ggaacttcaa
    40861 agcagtaccc tcttttcata aactatatga ataaagtttt taagtatgct ataaatattg
    40921 gtttaacatc tgataatcca acattaaatt tacttattcc aaagccacaa attaaaacag
    40981 aaaagaaatt aaaattatat acaaaagaac agttggaatt atttctaaat gaagtatctc
    41041 aagaacagaa tccatatttt aaaaacagag actatacgct ctttagacta ttagcattca
    41101 gcggatgtag aatcggcgaa atattagcac tcacttggga caatattaat tttaaaacaa
    41161 atgaaatggc cattaaaaaa actgtagctc gttcagataa atattatata tctgaaactc
    41221 ctaaaacaaa aaaatcaaat cgaataattt atttagatga aaaaactata aagcaactaa
    41281 aattttggaa gctcgaacaa agaaagtact tatttcaatt aggatttact aaagctaatt
    41341 atttgtttac caatgacgaa aataatttca caattaatca gtcagtggca gaaagataca
    41401 atatatatcg tgagcgtgcc ggcttacctt atatcggtct gcatggtttt agacatacac
    41461 atgcatcaat gctatatgag gcaggcgcag atcacaaaga agtccaagaa agaatgggcc
    41521 acgcaaatat aaaaactact atggacacat atacacacat tactaacagc aaaaaagaag
    41581 aaacaacaca aaaactaaca aattatatta acttctaaga aagtatggtc aaaagtatgg
    41641 tcaaaaaaat cccttactta gcaaaaaaag ctccatccct tgccacacaa gagatagagc
    41701 agtttattta ttaagcttct tgagctactg gataaacaga cacttcaatt atactgtttc
    41761 ctgataagct ctcaatgctt ctgaacccat gatattagtg cttttcttct attattaaat
    41821 atcttgtaat tttctattct ttctaaaaag tatggtcaaa gtatggtcac tattgataga
    41881 atattaatag tagtagaaag ctgatat
//
LOCUS       DACDEA010000018        40922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDEA010000018 DACDEA010000000
VERSION     DACDEA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.018"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV136_000986"
     CDS             complement(38..1189)
                     /locus_tag="IV136_000986"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8464992.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV136_000987"
     CDS             complement(1202..1639)
                     /locus_tag="IV136_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8464993.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV136_000988"
     CDS             complement(1654..3420)
                     /locus_tag="IV136_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8464994.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV136_000989"
     CDS             3684..4361
                     /locus_tag="IV136_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8464995.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IV136_000990"
     CDS             4364..5491
                     /locus_tag="IV136_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8464996.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IV136_000991"
     CDS             complement(5691..6131)
                     /locus_tag="IV136_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8464997.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IV136_000992"
     CDS             complement(6142..8175)
                     /locus_tag="IV136_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8464998.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IV136_000993"
     CDS             complement(8177..9880)
                     /locus_tag="IV136_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8464999.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV136_000994"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV136_000994"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8465000.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IV136_000995"
     CDS             complement(10963..11988)
                     /locus_tag="IV136_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8465001.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IV136_000996"
     CDS             complement(12197..13066)
                     /locus_tag="IV136_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8465002.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IV136_000997"
     CDS             complement(13525..15417)
                     /locus_tag="IV136_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465003.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IV136_000998"
     CDS             complement(15414..17141)
                     /locus_tag="IV136_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465004.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IV136_000999"
     CDS             complement(17157..17807)
                     /locus_tag="IV136_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8465005.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IV136_001000"
     CDS             18011..18307
                     /locus_tag="IV136_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8465006.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IV136_001001"
     CDS             18479..19099
                     /locus_tag="IV136_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8465007.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IV136_001002"
     CDS             complement(19191..20357)
                     /locus_tag="IV136_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8465008.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IV136_001003"
     CDS             complement(20369..20722)
                     /locus_tag="IV136_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8465009.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IV136_001004"
     CDS             20850..21872
                     /locus_tag="IV136_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8465010.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IV136_001005"
     CDS             complement(22311..22745)
                     /locus_tag="IV136_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465011.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IV136_001006"
     CDS             complement(22855..24714)
                     /locus_tag="IV136_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8465012.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IV136_001007"
     CDS             complement(24832..25992)
                     /locus_tag="IV136_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8465013.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV136_001008"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV136_001008"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8465014.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IV136_001009"
     CDS             complement(27739..28437)
                     /locus_tag="IV136_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8465015.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IV136_001010"
     CDS             complement(28394..30055)
                     /locus_tag="IV136_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8465016.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IV136_001011"
     CDS             30273..31673
                     /locus_tag="IV136_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8465017.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IV136_001012"
     CDS             complement(31925..33004)
                     /locus_tag="IV136_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8465018.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IV136_001013"
     CDS             complement(33090..34049)
                     /locus_tag="IV136_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8465019.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IV136_001014"
     CDS             complement(34061..35584)
                     /locus_tag="IV136_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8465020.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IV136_001015"
     CDS             complement(35594..36157)
                     /locus_tag="IV136_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8465021.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IV136_001016"
     CDS             complement(36163..37026)
                     /locus_tag="IV136_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465022.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IV136_001017"
     CDS             complement(37040..37936)
                     /locus_tag="IV136_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465023.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IV136_001018"
     CDS             complement(38111..39382)
                     /locus_tag="IV136_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465024.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IV136_001019"
     CDS             complement(39372..40889)
                     /locus_tag="IV136_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8465025.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11793 a         8578 c         7089 g        13462 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 ta
//
LOCUS       DACDEA010000019        40585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDEA010000019 DACDEA010000000
VERSION     DACDEA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.019"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            667..2277
                     /locus_tag="IV136_001020"
     CDS             667..2277
                     /locus_tag="IV136_001020"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8465026.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2569..3438
                     /locus_tag="IV136_001021"
     CDS             2569..3438
                     /locus_tag="IV136_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8465027.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3603..4874
                     /locus_tag="IV136_001022"
     CDS             3603..4874
                     /locus_tag="IV136_001022"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8465028.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4871..6166
                     /locus_tag="IV136_001023"
     CDS             4871..6166
                     /locus_tag="IV136_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8465029.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6265..6528
                     /locus_tag="IV136_001024"
     CDS             6265..6528
                     /locus_tag="IV136_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8465030.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6973..7422
                     /locus_tag="IV136_001025"
     CDS             6973..7422
                     /locus_tag="IV136_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8465031.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7479..8897
                     /locus_tag="IV136_001026"
     CDS             7479..8897
                     /locus_tag="IV136_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8465032.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8897..9757
                     /locus_tag="IV136_001027"
     CDS             8897..9757
                     /locus_tag="IV136_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8465033.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9931..10704
                     /locus_tag="IV136_001028"
     CDS             9931..10704
                     /locus_tag="IV136_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8465034.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10851..11216
                     /locus_tag="IV136_001029"
     CDS             10851..11216
                     /locus_tag="IV136_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8465035.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11364..12572)
                     /locus_tag="IV136_001030"
     CDS             complement(11364..12572)
                     /locus_tag="IV136_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8465036.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12591..12869)
                     /locus_tag="IV136_001031"
     CDS             complement(12591..12869)
                     /locus_tag="IV136_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465037.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13062..13718
                     /locus_tag="IV136_001032"
     CDS             13062..13718
                     /locus_tag="IV136_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8465038.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14160..14957)
                     /locus_tag="IV136_001033"
     CDS             complement(14160..14957)
                     /locus_tag="IV136_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8465039.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14954..16138)
                     /locus_tag="IV136_001034"
     CDS             complement(14954..16138)
                     /locus_tag="IV136_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8465040.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16163..17410)
                     /locus_tag="IV136_001035"
     CDS             complement(16163..17410)
                     /locus_tag="IV136_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8465041.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17625..19370
                     /gene="dnaX"
                     /locus_tag="IV136_001036"
     CDS             17625..19370
                     /gene="dnaX"
                     /locus_tag="IV136_001036"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8465042.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19390..19704
                     /locus_tag="IV136_001037"
     CDS             19390..19704
                     /locus_tag="IV136_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8465043.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19729..20325
                     /gene="recR"
                     /locus_tag="IV136_001038"
     CDS             19729..20325
                     /gene="recR"
                     /locus_tag="IV136_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8465044.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20389..21033
                     /locus_tag="IV136_001039"
     CDS             20389..21033
                     /locus_tag="IV136_001039"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8465045.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21048..21377
                     /locus_tag="IV136_001040"
     CDS             21048..21377
                     /locus_tag="IV136_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465046.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21391..22329
                     /gene="holB"
                     /locus_tag="IV136_001041"
     CDS             21391..22329
                     /gene="holB"
                     /locus_tag="IV136_001041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8465047.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22365..23189
                     /locus_tag="IV136_001042"
     CDS             22365..23189
                     /locus_tag="IV136_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8465048.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23182..23529
                     /locus_tag="IV136_001043"
     CDS             23182..23529
                     /locus_tag="IV136_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8465049.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23598..24470
                     /gene="rsmI"
                     /locus_tag="IV136_001044"
     CDS             23598..24470
                     /gene="rsmI"
                     /locus_tag="IV136_001044"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8465050.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24579..25700
                     /gene="dinB"
                     /locus_tag="IV136_001045"
     CDS             24579..25700
                     /gene="dinB"
                     /locus_tag="IV136_001045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8465051.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25754..26356)
                     /gene="nrdG"
                     /locus_tag="IV136_001046"
     CDS             complement(25754..26356)
                     /gene="nrdG"
                     /locus_tag="IV136_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8465052.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26671..28860)
                     /gene="nrdD"
                     /locus_tag="IV136_001047"
     CDS             complement(26671..28860)
                     /gene="nrdD"
                     /locus_tag="IV136_001047"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8465053.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29186..29953
                     /locus_tag="IV136_001048"
     CDS             29186..29953
                     /locus_tag="IV136_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465054.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29943..31985
                     /locus_tag="IV136_001049"
     CDS             29943..31985
                     /locus_tag="IV136_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465055.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32266..32415
                     /locus_tag="IV136_001050"
     CDS             32266..32415
                     /locus_tag="IV136_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8465056.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32426..33940
                     /gene="dltA"
                     /locus_tag="IV136_001051"
     CDS             32426..33940
                     /gene="dltA"
                     /locus_tag="IV136_001051"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8465057.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33937..35142
                     /gene="dltB"
                     /locus_tag="IV136_001052"
     CDS             33937..35142
                     /gene="dltB"
                     /locus_tag="IV136_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8465058.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35187..35420
                     /gene="dltC"
                     /locus_tag="IV136_001053"
     CDS             35187..35420
                     /gene="dltC"
                     /locus_tag="IV136_001053"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8465059.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35423..36691
                     /gene="dltD"
                     /locus_tag="IV136_001054"
     CDS             35423..36691
                     /gene="dltD"
                     /locus_tag="IV136_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8465060.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36832..37914
                     /locus_tag="IV136_001055"
     CDS             36832..37914
                     /locus_tag="IV136_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8465061.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37914..38717
                     /locus_tag="IV136_001056"
     CDS             37914..38717
                     /locus_tag="IV136_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8465062.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38787..39527
                     /locus_tag="IV136_001057"
     CDS             38787..39527
                     /locus_tag="IV136_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8465063.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39538..40542
                     /locus_tag="IV136_001058"
     CDS             39538..40542
                     /locus_tag="IV136_001058"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8465064.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13042 a         7108 c         8458 g        11977 t
ORIGIN      
        1 cttttgtcgc atctccttat tttttactta tgtgattcgt taccttcaat gaatccctac
       61 aaggcacttt tgttgaaaaa aaggtcatct ttgaaaaaat tagtggaatt gttttgtttt
      121 tgatattatt taatagaaaa gatatgccaa aaatccgaga actgtggact acgtttttta
      181 cgtaaaggag gtaaataagg gtgaaagaaa aagattgttt ctacgataag ctatataaac
      241 tgttgctgca agtaggaaaa agtagttctt tgttaggtgg gatattatct atcataatgt
      301 ttgttttatt tttttatcta ttatttcact taaaattttt cagtaacgat tttctgattt
      361 gcttaggttt aacgttagct atgtttatat caagcttatt atgtgggtta aatttttttt
      421 gcactcataa aggacttaag aaataatttc tcaaaggata tcgaaaaaga aaaaattgta
      481 agcggatagt attcatttga gtacttgact ttcccttagt cagcttgtat tatacacctt
      541 gggcaccctt ttagagggtc agaagtttac agaatttcgg ctccctattc ttattgaaga
      601 atagggagct ttgtttattg tctgagactt ttcctgcaca taaattaaaa aggagttttt
      661 gaacttatga caaaatatat ttttgtgacc ggcggtgtcg tttcatcaat cggaaaaggg
      721 attgttgcag cttctttagg acgcttattg aaaaaccgcg gcttaaaagt aactatccaa
      781 aaatttgatc catacatcaa cgtggatccg gggacgatga gcccatatca gcacggagaa
      841 gtattcgtaa cagatgatgg tgctgaaaca gatttggatc tgggccacta cgaacgtttt
      901 attgacatca atttgaacaa atactccaac gtgacgacag gaaaaatcta ttctgaagtt
      961 ttgcgtaaag aacgtaaggg cgaatacttg ggagcaactg ttcaagttat tcctcatatt
     1021 acaaatgaga tcaaagaaaa aatcatgcga gcagctaaaa tgactgattc agatgtgatc
     1081 attacagaag tagggggaac agtaggggat atagaatctt tgccattcct tgaagcactt
     1141 cgtcaaatga aagcagatgt cggtacagat aatgttatgt atatccatac aacattgatt
     1201 ccttacttga aagctgctgg agaaatgaaa actaaaccaa cgcagcacag tgtaaaagag
     1261 ctacgtggtt taggtatcca accgaatatt ttggtggtac gtacagagca acctgtttct
     1321 caaagcgtga aaaacaaatt ggcacaattt tgtgatgtcg aaccagaagc agttatcgag
     1381 tcgccagatg tagatacact ttactccatc ccattagttt tacaagcaca aggaatggat
     1441 caaattgttt gcgaccattt aaaactagat acaccagaag cagacatgac agaatggaaa
     1501 gaattagaag aacgtgtctt gaacttgaag aaaaaagtac ggattgcgct tgtcggtaaa
     1561 tatgtcgagt taccagatgc atatatctca gtagttgaag cattgaaaca ttctggtttc
     1621 gcctataatt cagacatcga gatcgattgg atcaaagcac aagaattaac gagagaaaat
     1681 gtagaagaac gtttgaaaga cgcagatgga atcttggttc ctggtggatt tggtgatcgc
     1741 ggagtagaag ggaaaatcga agcgattcgt tatgctcgcg aaaatgacgt tccgttttta
     1801 ggtatttgtc ttggtatgca gatggcttgt gtcgaatttg cacgtaacgt cgttggatta
     1861 gaagatgcgt catcagctga aacgaatcca gatacagcaa acaatattat tgatttaatg
     1921 gctgatcagg aaaacatcga gaatttgggt ggaactttac gccttggatt atacccatgt
     1981 aaaatcaaaa aaggaacgaa gacggctgaa gcatatgatg gagccgatgt tgtgcaagaa
     2041 cgtcaccgtc accgctatga gttcaataac aaatatcgtc aattgtttga agaacaaggg
     2101 ctagtctttt caggtgtctc accagataac cgtttagtag aaatcgtaga gctgccagag
     2161 aaaaaattct ttgtgggatg ccaattccac ccagaattga tttctcgtcc aaatcgcccg
     2221 caaaaattaa tcaaagcatt tgtcggagca tcattagcag atcacgaaac aaaataattt
     2281 ttgaacttga tagaaagaga aaagtagcaa gagaagggga taagcaattt tcttgtcact
     2341 tttctctttt tctgatttgt tattatttgt aggaaaaaag atagaaagca aaagactttt
     2401 ttcgattaaa gcgctattgg atattttatt ttttgcaaaa aacttcttga aaattttcat
     2461 tttcattgat tttagtagaa aactaccgaa atgtttggta aaatagggtc gttggttaag
     2521 aattgtctta acaaatttag ttttacaaaa cttaggagga attttattat gccagtagta
     2581 tcaggagctg aatttttaaa agctgctcgt aaaggcggtt acgctgtcgg cggattcaac
     2641 acaaacaact tggaatggac tcaagcaatc cttgaagcgg ctgaagcaaa acaagcacca
     2701 gtacttatcc aaacatcaat gggtgcagct aaatacatgg gtggttacaa aatctgtaaa
     2761 gatttaatca ctgatttagt tgaagcaatg aacatcactg ttccagttgc tatccactta
     2821 gaccatggtg attacgaagc tgctttggaa tgtatcgaat taggctatac ttcagtaatg
     2881 ttcgatggtt cacatctacc atttgaagaa aacttgaaat tagctaaaga cgttgttgaa
     2941 aaagcacatg caaaaggtat ctcagttgaa tgtgaagttg gttcaatcgg cggagaagaa
     3001 gacggaatca tcggtaacgg cgaattagct gatattgaag aatgcaaaca aatggttgct
     3061 acaggtatcg actacttagc ttgcggtatc ggtaacatcc atggtcaata tccagaaaac
     3121 tggaaaggat tagcttttga ccacttacaa gcaattgctg atgctgtagg tccagacatg
     3181 cctttagtat tacatggcgg atcaggtatt cctcaagatc aaatcgaaaa agctatctca
     3241 atgggtatct caaaagtaaa tgttaacact gaattccaat tatcatttgc tgcagctact
     3301 cgtaaatata tcgaagctgg taaagataaa gaaggtaaag gattcgaccc tcgtaaatta
     3361 ttagctccag gtaaagcagc aatcgttgaa gatgctaaat ctcatatcga ctggttcggt
     3421 tcagctaaca aagcgtaagc atccacataa cagatacgat atataggaat ggagaacgac
     3481 ttttgaagtc gctctccatt cttttttact taaaacggca gaccatgctc gtttcttttt
     3541 tttatctatg ataaaataca agcatatcca tgaaaaaaga ttaataaaca aggtggaaac
     3601 agatgaaaaa aatcgtaatc aaaggaaacc gacctttagc tggtgaagta acgatcagcg
     3661 gtgcaaaaaa tagtgcagtc gcattgattc cagctgcaat cttagctgat tccccagtga
     3721 tcttggaagg tgtgccggac atacaagatg ttcattcatt aattgaaatt ttagagatca
     3781 tgggcgcaac tgtccatttt tccaacaaca tactagaaat cgatcctcga gaagtcgtat
     3841 cgattccgat gccgcacgga aaaatcaata gtttgcgtgc ttcttattat tttatgggaa
     3901 cactgcttgg taaatttggc gaagcggtag taggcctgcc aggtggctgt tatttaggcc
     3961 ctagaccgat tgacctacac gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg
     4021 aacatggtgc catgtattta cggacagaaa ataaaggatt gcgtggcaat cggattttca
     4081 tggacgtggt ttcagtcgga gcaacgatca atgtgatgtt agctgctgta aaagcaaaag
     4141 gacagacagt aattgaaaac gctgcacgag aaccggaaat catagatgtg gcaactttac
     4201 tgaataatat gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt atcgaaggag
     4261 tcgaaacatt acatggctgt cggcatttca tgattcctga ccgcatcgaa gcaggaacct
     4321 atctagcatt agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac
     4381 atttggaaag ctttatagca aaattgcagg aaattggtgt ccatatgaaa atcagcgaag
     4441 atgaaatcga agtttacccc tcgaaagaat taaaacccgc aaacatcatg acctatcctt
     4501 atccaggatt tgccacagat ctgcagcagc cacttactgc gttactattg atgactacag
     4561 gcacttcaga aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg ccggaactag
     4621 cacgaatggg cgctgatatt accgtggaag gaaatatgat catcgtgaat ggtccaaata
     4681 aacttcacgg cacagaagtc gtggcttctg atcttcgggc aggtgcttgt ttagtgattg
     4741 ctggactatt ggcagaaggg acaacgacga tttacaatgt agattatatc ttacgcggtt
     4801 atgatcatat tatcgagaaa ctaacagcct taggggcatc aattgaaatg atagaggaag
     4861 tgggagctga atgagcgatt atttgacaat ggcagaacta gagaacaaaa cgttaaaaga
     4921 agtttatagt tatgccaaag aatttaaaat tccttattac agtaagatga ataaaaagga
     4981 attatctcta gctgtgattc gtgcacaagc agagaaacaa ggattttatt atatggaagg
     5041 aatattggat atcgttggtc aagatggtta cggtttttta cggccaatca attatggacc
     5101 aagtgcagaa gatatctata tttctgcctc acaaatcaga cgattcagtt tgagaaatgg
     5161 ggacaaagtg gcaggaaaag cccgtccacc taaagaatcg gaacgttatt atggtttgat
     5221 gcacgtggaa agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc
     5281 agccttaaca cctctttatc cgcaaagaca gcttcaactg gaaacaacac cgcaaagact
     5341 atctacccga atgatcgata tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt
     5401 cgcaccgcca aaagcaggga aaacttctgt attaaaagaa atcgcaaatg gtatcaccga
     5461 aaactatccg gaagtagaat taatcgttct actgattgat gaacgaccgg aagaagtaac
     5521 ggatctagaa cgaagcgtga aaggcgacgt ggtatcttct acatttgatc tgcagccgca
     5581 aaaccatact cgagtttctg aattagtatt ggaacgcgca atgcgtttag tcgaagataa
     5641 acgagatgtc gttatcttga tggatagtat tactagactt gctcgggcct ataacttagt
     5701 cgttccacca agcggacgta cattgagtgg cgggattgac ccagccgcat tgtataaacc
     5761 taagaaattt ttcggggcag caagaaacat cgaagaaggt ggcagtttga cgattttagc
     5821 gaccgcttta gtcgatacag gaagccgtat ggatgacgtg atctatgaag aattcaaagg
     5881 aactggaaat ctagaattac aactttcacg tgaattatct gaacgtcgga tcttcccagc
     5941 catcgatatc aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt
     6001 agaagaaatt tggaaacttc gaaaaaatat gactggagat tcattagaat atacggaaca
     6061 actgatccgc tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga
     6121 tgtctccttt ggtaaaaaaa atccgaccag aaatctgaaa agataattgc aaccttgact
     6181 gaaacatgtt aagatgttaa tgttgtaaat cgacaaatca actctggttc agcaaatgga
     6241 gcagggcaaa ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta ccacccagta
     6301 gtattcatgg attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa
     6361 gaaacagttg aatgggaaga cggtaataca tacccagtca tccgtgtcga agtaacttct
     6421 gactcacatc cattctacac tggacgtcaa aaattcacac aagcagacgg acgtgtggac
     6481 cgtttcaaca aaaaatacgg tctcaaagac gcaaacgctg cggaataata aaaacgctga
     6541 taactcaacg tttagcagac tatctgatag ggatagtctg tttttttatg ttttttgcca
     6601 atttaaaact tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa
     6661 aactagtata tagttttaat tatccacttg ataaaaacac cttatattaa cgaaatgaag
     6721 aggggttatt tcgaagtaaa aaaccattta cctaaaatta gaagctaagc tttcctttca
     6781 aggaaggctt tttgttttgt tatagacttg cgattgtttg tgagctagat cgttcatttt
     6841 aatgacttat acacgcggat gatgggtctt tttcctctgt tcattcataa tattgaacgg
     6901 tacagctagc gatgctatac tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg
     6961 gagaggtttt ttatgaggtt tgatcaattg atcgcagaag aactgattgt tttcgatcca
     7021 tcgatagatt caaaaggatc attatttgag cgaatagggg atgtgttgaa aaaaacaaat
     7081 aaggtaagaa atgttcaaaa agtcgttaag gcactattca aacgagagga ggagatctcc
     7141 acaggaatag aagatggatt tgggattccc catgcaaaaa gcaaatatgt gacaaaacca
     7201 gcactgtgtt tttttcatac agccaaatta acggactata gcgggataga tgaaatgcca
     7261 attgaatata tttttgccat cgtagttcct gaaaaatcag cagatatgca tctagagatt
     7321 ctcagcacac tctccagacg attgatgaat gcagatttta gaaagggaat aaaagaagcc
     7381 actacgccag cagatatcat cagacagatt agtcaagaat gaaaatagta gagtctttgt
     7441 ccgagtatcg atagtgaaga aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg
     7501 cttgcccgac tggaattgct catacttata tgtcggtaga aaaattagaa gtaacagcaa
     7561 gaaatctaaa ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat
     7621 ctgaacaaga aatcaaagag gcagatgcaa tcattcttgc agtagataaa gaaatcgaat
     7681 tggaccgatt tgccgggaaa aaagtaaagc gtgtctctac aagccgagca atcaaagaac
     7741 cgcaagtagt aatagacgaa gcattgagag atattggcgt attcgttgtg tctaatgaaa
     7801 aagaaccagc tgcaaatgag aaaaagccga caatctataa tcatttcatg aatggtgtga
     7861 attacatgct tccatttgtg atcgcaggcg gaatcatcat cgcactgagt tttgcctttg
     7921 ggattacagc ggctgatccc aactcagcag actataatgt gttagcagct gcattttcaa
     7981 ggatcggagg agatacagcg tttgccatga tggttcctgc gttaggcgcg gggattgcta
     8041 catcgattgc tggaaaagca ggttttgccc caggaattgt tgccggatta ctggcctcta
     8101 caggtggttc aggttttctt ggcggaatga ttggtggcgt gctagcaggg tatatctgtg
     8161 attttctggc aaataaaatt agcgtaaaaa aagaattttc tgctatttat cagctgattg
     8221 tcgtcccctt tttatcgatc ttaattattg ggcttttgat ggtatttgtg attgaacaac
     8281 cgatcacatg ggtgttggat gctttgacaa attggctgaa tagcttggga aatacatccg
     8341 ggctattgtt cggattactt atcgggatca tgatggcagc tgatatgggt gggccgatca
     8401 ataaatcgac ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg
     8461 ctgcttgtat ggctgcagga atgacacctc ccctaggtat tgctttagcc actttattat
     8521 tcaaacataa atttaccaaa gaagaacgga cttcaggtca atcatgctgg attttaggcg
     8581 cttcgtatat tacagaaggt gctataccgt ttgcggtatc tgacccgatc cgcgtgatac
     8641 ctagtttgat gacaggttca gcagtcgctg cagggatttc catggcagta ggagttactt
     8701 caatggcacc tcatggaggg atttgggtga tgctaattcc aaatgttatg aacaatttac
     8761 ctatgtatgt cttggcaatt gcatcaggta cagtcactac agcctttatg cttggactat
     8821 tgaaaaaaac agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa
     8881 atgaggtgga agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa
     8941 tcaatttgca gtaccagcct tcaatatttg cagctatgat atgatgaaag caatcatcga
     9001 agaagcagag agattagaag caccagtaat cttagaaatt catcctgatg aaatcgccta
     9061 tttaagtgat gagtttgttg cggcagtaag agcctatgct tatacaagta aactgccgat
     9121 cgttatccac ttggatcatg gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg
     9181 ctattcttcc gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg tacagattac
     9241 gaaaaaagtg gttgcgttag ctcatgcggt aaatgtctca gtggaggctg agcttggaac
     9301 gattgggaat aatggttctg ctgaaggagg tgctgaaaca atcatttata cagatcctga
     9361 tcaagcgaag atttttgtga aagaaacagg aatcgataca cttgctgtag caattggaac
     9421 agcgcatggc ttgtatccga aaaacagaac tccaaaatta aatatcgact tactgaaaga
     9481 attgaaccaa caaatcgatc ttccatttgt tttacatggc ggttcaggta atccggacgc
     9541 agaagtcagc gaagcagtaa aatacggagt tgccaaagtg aatttgagct cagatcttaa
     9601 aagcgtcttt ttcaatgctt tgagagaaat attgagtgaa aatccatcga tgtacgagcc
     9661 gaatgcaatt tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga
     9721 aattttgaac acagtaggca aggctagttt atactaaaaa taaaaatgtg tggtcaagag
     9781 ggtatgacaa tagtcttgtc ataccctctt aaccacacac gatgttcaga agtagttttt
     9841 tcgaaaaatt tcttcttatt tctcgaagcc aatcacttct gtcacaacct cttttttcct
     9901 gttactatat aaagtaaatg gggtgatttt atgttgaatg aagtggagaa tagacagcag
     9961 gagattgtga attatctaaa aaacaaccaa tttgcaagag tcaccgattt gatcgagtta
    10021 gtaaattata gcgaagccac gattaaacgt gaccttgttt ttctggaaaa aagtggattg
    10081 atccgtagag ttcgcggtgg cgccatgctt attgacaatc gaaaaattga cgtgccttac
    10141 ctgatgaaga tcaccaaatt agaagaagaa acagaaaaac gtaagattgc agatacagct
    10201 tcttctttga ttcgagatga tatggtctta ttcttggatt ccagtacaac ctctttacac
    10261 ttagtcagaa acctagcaag atttgatggt ttgcagatca ttacgaatgg catcgtcaca
    10321 gctgctatgt tgagtgaata tacaacggca aaagtatctg tattgggagg ggcaattgtt
    10381 ccgaaacgag caacaatcaa cggttcaaaa gcctataacg atgcattgtc atattatgcg
    10441 gatttagcga taatgagttg tcgcgggcta gatttcagcc aaggagtaac agaaactcat
    10501 gaaggagagg caatggtcaa aaaagctttt agaaaacaat cagaccggct tgccgtttta
    10561 gttacacagc aaaaaattgc acataaattc atgcatcaag ccattgcttt tcatgaaatc
    10621 gattatctgg tgacaagtaa agaactgaca aaagaacagt atgcgaaagt aaaggaacac
    10681 catatcgaat gtctgtacgg ataaattatt acgtgaatgt tctaagtggc ttcctcattt
    10741 ttagatagga ccttatcttt gctcgcttct ctcagccgat ggtataataa tgaaaaggtt
    10801 atcttgttag ataatcttac ttttttatcc aggagaaagg agtgcagaaa atgaaacatg
    10861 tgaaggcgtt ggtagtgaag gcaatcatga tctgggcgat tctgtgggtc gtgctgacag
    10921 gtttatacgg tgttagcttt atggattcaa cgattgttgg ggtaattatt gtagtcatga
    10981 tctatgtgct aggggatctg ttgatcttac gtaaagtagg gaacatcgca gcgacgatcg
    11041 cagatgctgg ttcagctgca gttatcttgt ggctctatct atattttatg aatgatacag
    11101 tcgatatttg gatgaaagta ttgattcctg ctctattgat cggtgtggca gaatggttct
    11161 tccacaagtg gttgctaagt caaggcatcg taccagatga acggaaaatg gaataaatca
    11221 acagttaaac tattcttatc gataaatgaa agctttaaaa ataaatagat gtttaattaa
    11281 gaataatcat aaactatttt tactcaaaaa accgctcaga ttattttcag atctgaacgg
    11341 ttttttttat ctattcatca agatcaaatt tcatttttca atgattcaat caattcgccc
    11401 tctgcttcat gactatgttt tcggccatta acaagataga gtgctgtaca tatcaatgga
    11461 atgactccgg caatcacata caatcctcta aatgaagtaa gagattttag catccccatc
    11521 aaataaggac caacgcctaa gcctaggtct aaaccaataa agtaggtaga taaagcaata
    11581 ctgattcgat gttcgcggac gatttttaaa caaacagcct gtccatttga cataaacgta
    11641 ccataaccta ggccgactaa tgctccagag ataagcaatg tcacactatt cgtcgttaca
    11701 ctcagtaaaa ataatcctgc tgttaagaat aaatagctag gatacatcac gtattgttct
    11761 cctttgacat cgaaaatcct tccagtcaat ggacgtgtaa atgtgatgac caaagcatat
    11821 acgacgaaga aaaatgaccc agcagcaact aagtggatta cttttgtata agaagataaa
    11881 aatgataaaa cacttgaata agaaagaccc atcaaaaagg caattcctgt aatgaaaagt
    11941 gctttctttt caataaaact attgatattc catgagcgaa gagatatttt ttgttcttca
    12001 gataatggaa tattttttac aggaaagaca aggcaggcaa tcgtaacaaa gaagaccagt
    12061 acaattgaaa accagataat gaaacgaaaa tcagtcatat tcagcaaaat cattcctaaa
    12121 aatggcccaa cagcggcagc taaactagta cttaatccat aataattgat gccttcccct
    12181 tttttagatg cagggatata tgcagtaacg atagcatttg tagctgtaga aactgtaccg
    12241 tagccgaatc cattcaagaa tcgaactaaa tacaatgccc cgatagttgg cgtataaaga
    12301 tatgctaatg ttgaaaggag atagaagatc gcgccgcctc ttaaggtaaa cttccgacca
    12361 aatagttcta actgtttccc catgaataag cgtgccagca atgttccgat gatatagatc
    12421 ccagaagcaa aacctgcttg gctcaagctg gcgtgtaagg tgtcttgcgc aatgactgcc
    12481 atgatgacca tcaacagata ataaatcagg tagacaataa aattgatcaa tgtgattcca
    12541 ataaaatatt tattaaataa tttcttttcc atgtatgtta tacattcccc ttaagctgaa
    12601 tatgctttta atttttgtat ttgaacttct acatcgtgat aattggatgg atagcgatca
    12661 acaagttggc gaataaataa atctcttgta gacataaaca gatttaaaaa ttcttcgcat
    12721 tcgtttgttg gattctcatc cactgctttg atcaattcga tcgagttttg atacgcatca
    12781 tttaaaaaga ctaaagaagc tttcatatca aatcttgttt gatatgctgt ttctaatcga
    12841 ttaatgattg tgttcatcca gtaattcatg ttttttccct ctattcttaa aaaattttac
    12901 ttctgcagta taacgaataa aaaaaaggaa aaaaatcgca taagtgatgg aaaaaggtac
    12961 ttaacaaatg ttaatatcat tagtaaaata cctttggact ttaaaaagtt ttaaagtact
    13021 gtatgatctt tttagatgaa agtttggatg ggggagcatc attggatagt agagtcttaa
    13081 aggaatattt ggataaaaat gattttccga ttatacgcaa aggatatcat aaatatttga
    13141 cattcgaagg gatggaagat tcccatacgt atatcttgaa agagggaacg gtaaaagcaa
    13201 gtgttatttc aaatgatggg agagaattta accttcgcta tatcaaagat cttgaaatcg
    13261 tctctctttt aaaagacgaa tattcacaat tcatcgattc gccgtataat attcgagttg
    13321 aatcagaata tgcagagttt tatcgaatca atcgtgtgcg cttttggcaa aatatcaacc
    13381 aagacccaaa gctacaacac tatgtaaaag agtattaccg ctatcgtctg ctttattcga
    13441 tgaaaaaaat gcagcaaatg ctattaaacg gaaagctggg agctgtttgt acgcaacttt
    13501 acgaattata tgatttattc ggtgtgcgga cagaagaagg gatgctgatc gatttttcga
    13561 tcacgaatga agaaatcgcc aaattttgtg gcatctcaac tgctagcagt gtaaacagaa
    13621 ttttaaaaca actgaaaaaa gatggtatta tcaccgttaa agaccatcat atcgttattt
    13681 tgaatatgga cttgttagaa gacaatatta tcttttgatg aacaaagagg ttgtgaaatc
    13741 agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt
    13801 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc
    13861 tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga
    13921 atattgtgac ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa
    13981 aaagctgttc tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta
    14041 acttggctgg acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa
    14101 ggggctgtga cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt
    14161 tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg
    14221 ccaccgccgc ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt
    14281 gtcagacgat cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg
    14341 tagatgcctg atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg
    14401 ataagagcca aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta
    14461 attttgccag tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat
    14521 gttgctgatt tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga
    14581 tcataaacat catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta
    14641 ccagaagtcg aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt
    14701 tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa
    14761 ttagatgtag tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc
    14821 tgttgttcca aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta
    14881 gctgtatctg catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa
    14941 aaaaattttt tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga
    15001 aaatcccaag agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct
    15061 ttcctgtttg tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat
    15121 aagtaacaag ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag
    15181 aagttcgcac accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg
    15241 aatagtcttt taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt
    15301 tttctgcttg gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga
    15361 aaggttgcac gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta
    15421 gttctttaaa agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt
    15481 cgatgtcacc tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta
    15541 cttcagcatc tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct
    15601 tattgaaaaa agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga
    15661 ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta
    15721 aacgtcccga tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg
    15781 catcagtcac gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat
    15841 tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct
    15901 cggcttcatt catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg
    15961 aaggttcttc tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt
    16021 ggctgacttc ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt
    16081 tgggatcaaa agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca
    16141 gctgcacacc tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt
    16201 ttggacagaa tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc
    16261 gcatttgtag cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga
    16321 attttccgga attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg
    16381 cagtttgttg attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg
    16441 cttgagaaag ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca
    16501 ttgctcctgc ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt
    16561 atccttcacg tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt
    16621 tcacagaatc atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac
    16681 gtaattcacg ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct
    16741 tagatggtac ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta
    16801 gcgctgttag gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt
    16861 tgtttttagg gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag
    16921 tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg
    16981 cgcttgtacc gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt
    17041 tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca
    17101 gtgttccatt gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag
    17161 cggtataatc gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat
    17221 ggacgactgt tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt
    17281 catctttgcg gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt
    17341 cgatgacttc tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga
    17401 taagacccat aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt
    17461 attataccat aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt
    17521 gcgtattgtt taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca
    17581 cgtataataa gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac
    17641 tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac
    17701 agacactaaa aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac
    17761 ccagaggaac cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga
    17821 acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta
    17881 cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat
    17941 ttatccgtga tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg
    18001 atgaggtaca tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc
    18061 caaaagaaag tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga
    18121 tcatttcacg gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac
    18181 atcttgaatt tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga
    18241 ttgcccgttc tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct
    18301 cgtttagtga aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg
    18361 acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg
    18421 aaacattgaa ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt
    18481 tacaatactg ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat
    18541 cgagtacgat cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt
    18601 tttcaatgat aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg
    18661 ctatttattt ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt
    18721 cagcagatgt agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg
    18781 aactaagaaa cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac
    18841 agccggtaca gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac
    18901 gagtatataa agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat
    18961 gggaagactt gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac
    19021 cagttgcagc tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa
    19081 gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca
    19141 aagattatgc gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa
    19201 attatttgac gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag
    19261 aaggagaaat cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa
    19321 aagcagagga actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag
    19381 tagataatta tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa
    19441 atgcaaaaag aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa
    19501 gccaccaatg aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa
    19561 atcaatgaag aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg
    19621 gcagtcaatg atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac
    19681 gcaaaaggtt tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca
    19741 gaaccaattg cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca
    19801 gcgacaagac tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct
    19861 aaatctttgt taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca
    19921 gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg
    19981 gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat
    20041 catgttctcc atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt
    20101 tcttctctgt tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat
    20161 gcaacaacag agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt
    20221 atcaaagtga ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat
    20281 gaagttactt tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg
    20341 ttttttcaga tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta
    20401 tttattacaa tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac
    20461 cctcttttga aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt
    20521 ccgattgctg aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa
    20581 cgtacggaag cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg
    20641 cctgctctgg caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct
    20701 tatcaaggtg caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct
    20761 acagaaggta ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta
    20821 agaagaatca agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa
    20881 ttccaccagc gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc
    20941 cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt
    21001 attgaacagt atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt
    21061 tagcgattat ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata
    21121 tccgtgccac aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta
    21181 tcgtgggtat tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg
    21241 aatcaagaaa gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg
    21301 tcccttatcc agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag
    21361 gattccatca attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg
    21421 caaccgatcg tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct
    21481 tatctttttg aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag
    21541 cacctgttct gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag
    21601 cggatcgaaa agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc
    21661 aaagtcgatc agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga
    21721 aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg
    21781 ttgaaattct tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta
    21841 ggaagaattc tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg
    21901 aaagaagcac tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt
    21961 ttggctttct taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt
    22021 aatgatgcta aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa
    22081 gcctttatct atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt
    22141 acaggattat ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac
    22201 ggcgaatatg gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag
    22261 cagaaactac gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg
    22321 attttttagc ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag
    22381 gtgtccgata tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg
    22441 tttatagtga caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc
    22501 ttcctaaaaa agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata
    22561 aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat
    22621 tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg
    22681 aatacacatt tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact
    22741 ttagagaatt ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa
    22801 ttggtgtccg cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat
    22861 gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa
    22921 gtttatcttt gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc
    22981 aatatgagag cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa
    23041 ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta
    23101 gtattcggct gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat
    23161 ttatccaagt aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta
    23221 gaatcagagt tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag
    23281 atcgtagaaa aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg
    23341 gaattgaacc aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct
    23401 cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga
    23461 tcaagaaggg acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc
    23521 ggtcgatgac caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat
    23581 taaggagatt gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg
    23641 atttatatct tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc
    23701 gtatgctgca agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat
    23761 tgaatcattt tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac
    23821 gtgtccctca attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg
    23881 caggaatgcc ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag
    23941 ggatttctgt gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag
    24001 ggctagtgcc tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga
    24061 aagaaacatt gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt
    24121 atcggatcac tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt
    24181 tgtgtcggga attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac
    24241 tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt
    24301 tgttcgaaat gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag
    24361 aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa
    24421 atgggctgaa aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa
    24481 gttgtctata atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac
    24541 gcgaacgaat gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt
    24601 tacataatga tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg
    24661 ttgaagaacg agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca
    24721 gtgacactgg tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa
    24781 ttcattcagc aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc
    24841 ctggcgacca tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt
    24901 atacagatat tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata
    24961 aaatcaattg caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga
    25021 gtgtccagct aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat
    25081 cagactatca aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt
    25141 taaaagcttt gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc
    25201 atgagctagg gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc
    25261 gaaattttgg aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc
    25321 cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt
    25381 tgcaaacgga agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag
    25441 cactgagaag agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact
    25501 attctactgt gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct
    25561 tttatcaagc aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt
    25621 tagggattac tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat
    25681 gggaaaatca agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct
    25741 cccaaagctg tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat
    25801 ccgtacattt ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga
    25861 tccgctggtt gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc
    25921 gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta
    25981 gcaattcatc aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt
    26041 ttcggatacg tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa
    26101 gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt
    26161 caggtgtgaa tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc
    26221 aggcaaataa gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat
    26281 taaacggttt gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg
    26341 gttttggatt tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa
    26401 gataaaatct actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa
    26461 agcttgttct tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt
    26521 gaaaaaagac ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg
    26581 cagtaataag cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat
    26641 tacttgatgc aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat
    26701 ttctttatgg cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt
    26761 acgtttgact acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc
    26821 tcgttctgtc ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt
    26881 attcgtacca agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt
    26941 cggattttgt tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt
    27001 tggataatcc ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata
    27061 gtggaaactg tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc
    27121 tgtatccaaa cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa
    27181 atgataacca tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc
    27241 aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc
    27301 aacttcatac aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc
    27361 attgacagaa tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg
    27421 cgcatttgct ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat
    27481 gatttctaag cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat
    27541 cgcgattctt ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc
    27601 ttgaccattt tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa
    27661 acttccagtt aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt
    27721 tgcacattcc aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac
    27781 accttttttc aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa
    27841 tccattgatt cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc
    27901 tagtccaaat cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat
    27961 ttcatattcc aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata
    28021 ttctttttgg cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata
    28081 gttcaattct gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata
    28141 ttgacttgac gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga
    28201 ttttggcgat tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa
    28261 atcaatcaag cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg
    28321 gatatcgcct ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga
    28381 ttttccaacg atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt
    28441 tgcgttttcg ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat
    28501 atctgttgct ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc
    28561 taattcatat tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat
    28621 tttgacattt tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg
    28681 aattttttca taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag
    28741 tgcttcattg atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt
    28801 catctgggat gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat
    28861 tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca
    28921 actaaatatc ggctatatat agtattcaat gtgctgtata attggatttc tatactatat
    28981 gtagtgtata ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg
    29041 catatttaat tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa
    29101 gtgacaaaaa cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat
    29161 gaaaaatgaa agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa
    29221 aagctacggc agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga
    29281 agaaggcgag tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa
    29341 tatcttgtcc acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat
    29401 cacaaaaatg aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt
    29461 tcaagagttc aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc
    29521 agttgaacgg atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt
    29581 gaatatagaa gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg
    29641 aattgcagct gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac
    29701 tggctctttg gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca
    29761 aaaagaaaaa gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga
    29821 tagaatttta tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag
    29881 aagagagttc tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat
    29941 gaatgctgta taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt
    30001 attttttcag tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg
    30061 atcaatcttt agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg
    30121 gtggatttat cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct
    30181 tgaatagaag gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga
    30241 tatcaggtat atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga
    30301 tattactcgg tattattttt tccaaactct tttcaatgat tttggttcga atgatggata
    30361 tggatcttac tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt
    30421 ttttcatcat ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa
    30481 tgattcgaag ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa
    30541 ggcaccgatt actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta
    30601 gtcattttag ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt
    30661 tcttttttcc ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt
    30721 atacatttcg gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca
    30781 attttttgat aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc
    30841 tgattacagt cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta
    30901 ctattttatc ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga
    30961 cagagaaaaa aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca
    31021 ccttgcatta taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc
    31081 aaactgctaa tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt
    31141 tgccgaacat ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc
    31201 tttttcggaa tatttcatta tatggatcta gtattggact gccagataat caagaattga
    31261 agattcaaca aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg
    31321 tactgattgt agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca
    31381 atgtagatgt atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt
    31441 cgataaagtg gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag
    31501 gagaaattag cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag
    31561 aaagctctcg tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa
    31621 tcggtatcaa tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta
    31681 gtattttgac agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat
    31741 tgactaagct ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac
    31801 ttaccttctt tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct
    31861 tcactcagta tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga
    31921 tccttatcta tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg
    31981 aatgatatac ggaataagac aagataataa atagaactaa attttgaata ccatttattt
    32041 ggaataagag gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa
    32101 agtacgagaa attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat
    32161 gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc
    32221 aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt
    32281 tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca
    32341 gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc
    32401 tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga
    32461 ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg
    32521 tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga
    32581 tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt
    32641 tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct
    32701 atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga
    32761 tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt
    32821 aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc
    32881 tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt
    32941 tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc
    33001 attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct
    33061 gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc
    33121 cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc
    33181 tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt
    33241 gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac
    33301 ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact
    33361 agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt
    33421 ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag
    33481 cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa
    33541 tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt
    33601 ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga
    33661 tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata
    33721 ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca
    33781 gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta
    33841 tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat
    33901 cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct
    33961 tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct
    34021 ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta
    34081 tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg
    34141 tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga
    34201 tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct
    34261 ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt
    34321 aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt
    34381 cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat
    34441 aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg
    34501 ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat
    34561 tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca
    34621 tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc
    34681 tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc
    34741 aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg
    34801 tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa
    34861 ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg
    34921 ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg
    34981 atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct
    35041 aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc
    35101 ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt gacatattaa
    35161 tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa
    35221 taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc
    35281 ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag
    35341 tccctgtatc tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag
    35401 caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt
    35461 tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa
    35521 tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc
    35581 gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt
    35641 aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg
    35701 tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc
    35761 tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt
    35821 taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga
    35881 ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt
    35941 aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa
    36001 agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa
    36061 gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca
    36121 tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca
    36181 aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca
    36241 tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt
    36301 ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga
    36361 agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg gactatctca
    36421 agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa
    36481 tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca
    36541 tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa
    36601 tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca
    36661 tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt
    36721 ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa
    36781 tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca
    36841 aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa
    36901 gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac
    36961 ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct
    37021 ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa
    37081 ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa
    37141 acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca
    37201 cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca
    37261 agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt
    37321 gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc
    37381 gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt
    37441 ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca
    37501 caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca
    37561 ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt
    37621 gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa
    37681 atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg
    37741 catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat
    37801 tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa
    37861 ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata
    37921 atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg
    37981 gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt
    38041 ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag
    38101 tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg
    38161 tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg
    38221 gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa
    38281 tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt
    38341 caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat
    38401 cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc
    38461 aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat
    38521 atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta
    38581 tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt
    38641 atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag
    38701 acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag
    38761 gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt
    38821 atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt
    38881 tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc
    38941 tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac
    39001 agtattactg gcaacttcat cctaagagaa atccggagac attgctttca atttatggac
    39061 acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc
    39121 cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa
    39181 tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg
    39241 tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt
    39301 tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt
    39361 caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc
    39421 tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag
    39481 ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg
    39541 atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg
    39601 ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc
    39661 atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat
    39721 ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac
    39781 ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta
    39841 acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc
    39901 cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac
    39961 ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat
    40021 gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt
    40081 gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt
    40141 cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac
    40201 gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat
    40261 tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct
    40321 atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc
    40381 tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag
    40441 aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt
    40501 ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa
    40561 tccacatata tggattccgc tattt
//
LOCUS       DACDEA010000020        40528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDEA010000020 DACDEA010000000
VERSION     DACDEA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.020"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IV136_001059"
     CDS             <1..135
                     /locus_tag="IV136_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8465065.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IV136_001060"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IV136_001060"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8465066.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IV136_001061"
     CDS             complement(2412..2663)
                     /locus_tag="IV136_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8465067.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IV136_001062"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IV136_001062"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8465068.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="IV136_001063"
     CDS             3613..3831
                     /locus_tag="IV136_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465069.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="IV136_001064"
     CDS             complement(4120..4632)
                     /locus_tag="IV136_001064"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8465070.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IV136_001065"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IV136_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8465071.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7519)
                     /locus_tag="IV136_001066"
     CDS             complement(7085..7519)
                     /locus_tag="IV136_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8465072.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="IV136_001067"
     CDS             complement(7529..8317)
                     /locus_tag="IV136_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8465073.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="IV136_001068"
     CDS             complement(8314..9255)
                     /locus_tag="IV136_001068"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8465074.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="IV136_001069"
     CDS             complement(9432..10019)
                     /locus_tag="IV136_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8465075.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IV136_001070"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IV136_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8465076.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="IV136_001071"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="IV136_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8465077.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="IV136_001072"
     CDS             complement(12936..13871)
                     /locus_tag="IV136_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8465078.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..15016
                     /locus_tag="IV136_001073"
     CDS             14057..15016
                     /locus_tag="IV136_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8465079.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15102..16196)
                     /locus_tag="IV136_001074"
     CDS             complement(15102..16196)
                     /locus_tag="IV136_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8465080.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16385..17146
                     /locus_tag="IV136_001075"
     CDS             16385..17146
                     /locus_tag="IV136_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8465081.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17261..17701)
                     /gene="fabZ"
                     /locus_tag="IV136_001076"
     CDS             complement(17261..17701)
                     /gene="fabZ"
                     /locus_tag="IV136_001076"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8465082.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17921..18676
                     /gene="fabI"
                     /locus_tag="IV136_001077"
     CDS             17921..18676
                     /gene="fabI"
                     /locus_tag="IV136_001077"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8465083.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18786..19799)
                     /locus_tag="IV136_001078"
     CDS             complement(18786..19799)
                     /locus_tag="IV136_001078"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8465084.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19964..20884
                     /locus_tag="IV136_001079"
     CDS             19964..20884
                     /locus_tag="IV136_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8465085.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21164..21664)
                     /locus_tag="IV136_001080"
     CDS             complement(21164..21664)
                     /locus_tag="IV136_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8465086.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21771..22289)
                     /locus_tag="IV136_001081"
     CDS             complement(21771..22289)
                     /locus_tag="IV136_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8465087.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22290..23645)
                     /gene="rph"
                     /locus_tag="IV136_001082"
     CDS             complement(22290..23645)
                     /gene="rph"
                     /locus_tag="IV136_001082"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8465088.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23648..24469)
                     /gene="racE"
                     /locus_tag="IV136_001083"
     CDS             complement(23648..24469)
                     /gene="racE"
                     /locus_tag="IV136_001083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8465089.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24631..25287)
                     /locus_tag="IV136_001084"
     CDS             complement(24631..25287)
                     /locus_tag="IV136_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8465090.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25303..25950)
                     /locus_tag="IV136_001085"
     CDS             complement(25303..25950)
                     /locus_tag="IV136_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8465091.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25963..26778)
                     /locus_tag="IV136_001086"
     CDS             complement(25963..26778)
                     /locus_tag="IV136_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8465092.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26792..27529)
                     /locus_tag="IV136_001087"
     CDS             complement(26792..27529)
                     /locus_tag="IV136_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8465093.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            27783..28793
                     /gene="trpS"
                     /locus_tag="IV136_001088"
     CDS             27783..28793
                     /gene="trpS"
                     /locus_tag="IV136_001088"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8465094.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            complement(28963..31383)
                     /locus_tag="IV136_001089"
     CDS             complement(28963..31383)
                     /locus_tag="IV136_001089"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8465095.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(31388..32431)
                     /gene="pheS"
                     /locus_tag="IV136_001090"
     CDS             complement(31388..32431)
                     /gene="pheS"
                     /locus_tag="IV136_001090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8465096.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            complement(32740..33081)
                     /locus_tag="IV136_001091"
     CDS             complement(32740..33081)
                     /locus_tag="IV136_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465097.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            complement(33150..36872)
                     /gene="addA"
                     /locus_tag="IV136_001092"
     CDS             complement(33150..36872)
                     /gene="addA"
                     /locus_tag="IV136_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8465098.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            complement(36869..40396)
                     /locus_tag="IV136_001093"
     CDS             complement(36869..40396)
                     /locus_tag="IV136_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8465099.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
BASE COUNT        11497 a         8701 c         7065 g        13265 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14401 aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg gccatcagga
    14461 aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata attatcctga
    14521 tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc atcataaaag
    14581 tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta aacccaacgg
    14641 ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct tccctaaaaa
    14701 cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga aagccattag
    14761 taaccaacat gatattgata tatattttgc ggaccctgga acaccttctc aacgcccatt
    14821 aaacgagaat tctaacggga ttctgcgtcg taatggactg ccgaaatcaa tggattttag
    14881 aaaagtgaat cagacattta tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa
    14941 atcattgaat tacagaacac caatcgaaat atttttgagc tatgtacaag aagcatttta
    15001 ttctagctta atttgacaaa tcataaccta taaaaaaaca aaataaaaaa gcagaaataa
    15061 ctggctattt ctagcggatt atttctgcaa taactaatta tctatttttt attttcaatt
    15121 aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga ataattcgtc atcggagatt
    15181 cttcctacaa cattgacacg tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct
    15241 cctttgtatt gaccgtaccc ttcattgtca atcagtacat ccccatgatg catatctcgc
    15301 gtattatgag gcgggaaatc tttatctttg tagtatacac gagtcatcgt tgaacgaagg
    15361 atatattctg agcgatcccc tcgataacta tgaaggttat caaacaagca gattcgttcg
    15421 ttttctgtaa taccttcttc cgtatctact ttcaatgttg gataatccgc gttaaatgct
    15481 tctgccattt ctttcaattc ttcttcactt gcgtacgcat ttccaataga aatatcatca
    15541 atcaatcctg taaggattaa atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct
    15601 tccagcgtgc acaagccatc ttgcgtaggc caagggccaa actcagcaga ttgcgaattg
    15661 acgaatgcca tcgtattcaa attatatttt ctgaattttt ctgaacaaaa aacaaagtgt
    15721 tcgtatccta aaccagagta tcgatgagga taaaaattat gagaacctaa taaattatcc
    15781 gtgtttggcg aataactcat gatgttgtca acgtaattcg tgcccgaact catattgatc
    15841 tcgattttga taccaaaagg attgcgcgtc atttttgctt cttctgctcc tgtgaatccg
    15901 atatctaagc ggaccccgta ggcacccatc ttatggaaaa aagataagtc atcgtaagaa
    15961 atctccaact gttcaaataa cgctggattg atatcaacca ttacttccat gcctaggcta
    16021 ttcgcgtaat caactacttc tttaaattct gaaagaactt tttctttatc atcattgatc
    16081 tgcagcaaac tggtaaaaac ccgtttatac ccatacttat gagctaaatc caagtacgct
    16141 ttgtcctttt caaatgttga ccgttctgga taaatcgaga ttcctaattt tcccatatta
    16201 ccatttcctc ctgcatcttt tcgtttttgt atccacttac attatagcaa acacaaagta
    16261 aatggtctac accttttctt tttcttttaa aataaatgat aaattccaat aatttaagta
    16321 ggtattttcc ctctattttg ttatcattaa agataacaaa atagtaaatt ggaaaggaac
    16381 agttatgaag aaaaataaag ttattttaag tattagttta gcagcagcat tgctgtttgc
    16441 tgctggttgt tctgcaaaca acacgacttc ttctagttcg tctacagaaa gttcaaaaag
    16501 tacatctact gcggaaagca cgactgccag tagcgtcgat ttgaacgcat tggaattacc
    16561 acaattgacg aaagatgtcg ccgaaaatga agatctggtc gaaatggtga cgaataaagg
    16621 cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac
    16681 acatgcaaaa gacggttact acaacggact gactttccat cgcgtcatca aagatttcat
    16741 gatccaaggt ggcgatccta aaggcgatgg cactggaggg gaaagcattt ggggagaagg
    16801 atttgaaaca gagatcaaca accaccttta taatataaga ggtgcgttat ctatggctcg
    16861 ctcacaagat cctaacagta acggcagcca gttctttatc gttcaaaata cagatgatat
    16921 gcatgatggt cttttaaaag acgactatcc tcaagcaatc atcgacgctt ataaaaacgg
    16981 cggttatcct tcactagatg gccattatac tgtcttcggc caagtgattg aaggaatgga
    17041 tgtcgtggat gctattgcaa atatcgaaac agatagttca gacaaaccaa aagaagaagt
    17101 aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg
    17161 aatcctgcat agatgacaac gtatgctagg attcaggcgt cttcttttta cataaaaagc
    17221 aagtaagctt ccttttgttt tgctatatca ggttttatag tcaagcattt tggttgacga
    17281 tgaacgtgaa ctcgcactca cagacttttt tatcatctac atatgctgca gataacgctg
    17341 taccgactga tcccttcatt ttggtgattt caaaatgaca tttcagcaca tctcctggaa
    17401 caactttttg acggaatttg gccttattga tcccaccgat ataagctctt tttcctagaa
    17461 actcttctga ttttaggata agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg
    17521 cacccggcat cactggattg tcaggaaagt gaccttggaa aaaatcttca ttgattgtta
    17581 cattttttgt cgcaacgatc ttttttcctg gttctaaagt atctacataa tcaatataac
    17641 aaataggata acgattagga atcaattcca ttacttcagt agcagataat acgtaagaca
    17701 taattaaact ccttctttca ggcaattgcc attttcatta ctttgactat caaagtatca
    17761 tattcctaaa tatttagcaa tcttttttta atttaagaaa aaatagtttg acaatcaaaa
    17821 cattttgact aataattaag aaatcaagaa gttattttag ctattattct ctattttttt
    17881 acgactatca aatttttatc agaaacaaag gagagtcatt atgtcatttc tagaaggaaa
    17941 aaaaatcgtc attatgggcg tcgctaacaa aaaaagcatt gcttggggtt gtgctgaagc
    18001 aatgttgaaa caaggggcag aaattatcta tacttaccaa aatgaacgaa tgaaaaaatc
    18061 attagtcaaa ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt ctagcgacga
    18121 aagcatcgaa aaagcaatga atgccatcgg tgaatatgct ggggagatcc acggccttgt
    18181 ccatgcagtt gcttacgcca acaaagaaga attgtccgga aatgtgtctg atatttccag
    18241 agacggctat caattagctc aagatatcag cagctactct ttaattgctg tatcaaaata
    18301 cgcacaaccg tttcttgcac caaacagcgg gatcgttagc atgacttatc taggttcaga
    18361 acgcgcaatc ccgaattata acatgatggg tattgcgaaa gcttctcttg aagctgctat
    18421 ccgttatttg gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa tctcagctgg
    18481 ggcaatcaaa acattagctg tcacaggtgt taaagactat caaaaactga tccagttatc
    18541 tgaatctcgt acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta atgcttgtgc
    18601 tttcttaatc agcccacttt ccagcggaat catcggtgat attatctacg ttgacaaagg
    18661 tgtccatctt tcataaactt ttgttgcatt ctttaaagta aaacatataa aaatcaggaa
    18721 aaaacttttt aaagtctctt cctgattttt tctttttacg tttttttcaa ctcattactt
    18781 tgaaattatt ccacgacttc aatccagcct tctggtgctt ctatgtctcc aaattgaata
    18841 cctgtcaatt catcatacaa ccgttttgtt actggtccta cttccgtttc actataaaat
    18901 acatgaagat tgttattgtg ataaatccca ccgattggag aaataacagc cgctgtacca
    18961 catgcaccag cttctgcaaa ttgatctaga tcatcaatga aaatatctcc ttcaattgct
    19021 tgcagtccta gtcgttcttc agctaactgc aacaaggaat atttcgtgat acttggtaag
    19081 atagagggcg attgcggagt gatgaatttt ccgtcttttg taattccaaa gaaatttgct
    19141 gcacctactt cttcgatttt cgtatgtgtg gctggatcaa gataaacaca atctgagtaa
    19201 tccagttctt ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc gccgactttt
    19261 gctgcaccag tccccttagg agcagcacga tcgtaatcag atacaataaa attcgttggt
    19321 tttagtccac ctttgaaata cgctcctact ggcgtacaga agacgacaaa taaatattct
    19381 ggagctggat ggacaccaat gttttcaccg accccaatca gtaaagggcg caagtataat
    19441 gtagcgcctg ttccataggg cggcacaaaa tcttcatttg cttttaccac ttgcttgaca
    19501 gcatcaacga acatcttttc agggaccatt ggcatcagca atcgtctagc actttgattc
    19561 atccgtcgac tattttgttc aggtcgaaag agattgacct tgccgtcttt tcgacgataa
    19621 gcttttagac cttcaaaaca ttgctgacca taatgaagcg ctggagagcc ttcatgaatc
    19681 gtcaggtaat tatcttctgt caattctcct tcttcccatt gtccgtcttt ccatttggca
    19741 ataaaacgcc aaggagtttt gatataagaa aaacctagat tgttccaatc gatttgcata
    19801 tacttaccac cttcgttatt ttctccacat tttatcatta tccatattat ttgtcatttt
    19861 attttcagct tttttccttc attatagtta tttttgctat ttcctttttt gaaaacttct
    19921 tggtacaata cagaaagaat caaattttag aaaagaggat tttatgtttt cattattagc
    19981 aactgccggt acgacagact cttctggcga gacattgaca gatcaagctg tcagcaaagt
    20041 aaatgcgttt cagcggtttt gggacgggat tgattgggac gccattttgg ctgcctttat
    20101 ccaaaaaagt ttatatctga tttttttgat cgtccttttt tggattcttc accgtgtggg
    20161 aaaatatctg attgataagt cttttaatca gtattctaaa aaagccattc taaacgagag
    20221 ccgcttaaag actttacata gtcttttaaa tactattttt cattataccc ttggtttctt
    20281 ttttatctat gcaattttat ctgctatcgg tgtgccgatc ggatcacttt tggctggtgc
    20341 cggaattgca ggtgtggcaa tcggtcttgg tgcccaaggg ttcatgagtg atgttattac
    20401 cggatttttt attatcatgg aacaacagat ggatgttggg gactacatca agctagcaaa
    20461 cttaacgatt gaaggaaccg tcgtatctgt tggtatccgt acccttcagc tgaaagcaac
    20521 ggatggtact gtccatttca tcccgaatcg aaacatcacc acgatcagca atctctctcg
    20581 tgcaaatatg caagtacttt tagatattcg gattgttcct gaagaaggat atgacaaaat
    20641 ctatgagatc attgatcgag taaatcgtac attagctgaa aaataccaag aagagctcca
    20701 aactgaacct tctatctttg gattagtgga tcttggaaac agcaattttg cgattcgtac
    20761 ggtctgctac gcattgaacg gaaaacaata tgccttaaaa gaagagttct tgagctccta
    20821 tgtcaaagaa ttgacagctt ctggtttcac gattccgaac agtccaattg ctgtcggaaa
    20881 ataatttgga caaacgatgc aacgaatctc actaaaaaga gcgtgggaca aaagtaaaga
    20941 acacttttgt cccacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    21001 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
    21061 tggagttaaa cgcttttgtc ccagcctctt tttcaagata aagtcaaaac tggtacgctg
    21121 ttttttctac ttattttttg attatctttt agtttctcgt tctttagctc acgatatcga
    21181 tttgttcttt taattcttcg attttcggag acaatatatt acgacgttct aattcatgca
    21241 ccatgaagtt tacggcttta agcagctctt ttcttgtaat gattcctttg aaaatccgat
    21301 cttctgtgac aaccggtaaa aaaggcgaat cgactaacaa atggagtact tcttctagtt
    21361 cccaattttc atcaattgtc ggaacatctg tttctgtcac atcagcaacc gttagtcctt
    21421 ctaacgggtc catgctgatt gtttgcaagt caatcatttt attgaccaca tctgataaac
    21481 ttactagtcc tacaaagtga tcatcttttc ctaatacagg aattttggaa taaccgacct
    21541 tcgataacac aagtaaacca tgggacaatg gatgatgata cataacattt gccacatttt
    21601 ctgcaggaat caaaaacgtt tcctgatttt ccagcagcat ttctcttact ataggtccaa
    21661 tcatctgatg gcctccattt ctatttcatt caacactact attttaccga aaccggatgc
    21721 aaaattcata aaataaagct tatttttatt aaaaattaga gtttttttat tcagcgctct
    21781 ctcgaaaaag aaaaggccaa ctcttcaact gcctgatggg cacggttata gtattgaaca
    21841 gaaatttggt catctaaact ttcgataaga gcatatgatg ggatttggat cgggcctcta
    21901 ggctgagaaa tgctaccagg attcaaaaac aggattttct gatccatctc acagcctatt
    21961 tgatgggtat gaccaaaaaa caccatatcg gcagttgcag ctcgagcttc gattgctaac
    22021 tgtgtcaaac caaatcggac attcgataaa tgaccgtgag tcatgaatac tgtatcctga
    22081 ccagtttgga tcactttttt ctgttcgaat cctggtccat aatcacaatt accttgaacc
    22141 acttggaatg tatcccataa tgggtcagaa gcttctaatt ccgaatcacc acaatggaaa
    22201 aagatatcca cttcagaacg ataacgatct actaagtcaa cgaggatatc tctgtctcca
    22261 tgattatcac ttactactaa ataccgcatc tatctctctc cttccagcca ttgtttccac
    22321 tcggcactca attttttcat tgcttgagcc cgatgactga tcttgttttt ttcttcgctc
    22381 gataattctg ctgctgtttg tttcttacct tctgggataa ataaaggatc atacccaaag
    22441 ccattttcgc cagatggtat acgggctact cgaccgggcc attccgcttc tactacaagg
    22501 ctgtcttttt tgggtgccgc gaatactaac gtacaatgga attgtgctgt tcttttttca
    22561 tccggcacat ctgtcaattc atataataat ttggcgttat tccccgcatc acttttttgt
    22621 tcccctgcaa aacgtgcaga atagattcct ggcataccgc ctaaagcatc cactttcaaa
    22681 cctgaatcat ctgctaagac tggctgatca agcaattgtg caatcgtttc tgcttttaat
    22741 cgggcatttt cttcaaatgt gctgccagtt tcttccacat ctggcaattc aggatagtca
    22801 agtaatgttt ttacatgata tcccgcttct ttgaacatat ttcgaaactc ttctgctttt
    22861 cctggatttc ttgtcgcaat gatgattgtt tttgtttctt ctatccgctc atcattttga
    22921 gcaaactctg ttagcaaagc atgtttttgc tctttgatca aatcttcaat cgcgtttttt
    22981 ccaaaaaaga gcatttcatt caactgttca ccatcaaacg ttgcttcttc tccagttcct
    23041 tgaatctcca cgaattttcc agattctgtc attacaagat tcatgtctac aagtgctgcg
    23101 gaatcttctt ggtaatccaa atctgtgacg caagtcccat ctggtaagat tcctacgcta
    23161 actgcagcaa gatgttcttt tattggatct tctgacaatt ctttttctct taaaagtttt
    23221 tcaatggcta atttcaatgc cacaaatgcc ccagtgatac ttgctgttct agtaccgcca
    23281 tccgcttgga tcacatcgca gtctacgatg atactacgtt ctcctaattt ttccaagtct
    23341 actactgcac gcaaggaacg accaataaga cgctggattt ccattgttct accagaaagt
    23401 ttccctttgc tgctttctct acgatttctc gtactcgttg ctcgaggcag catactatat
    23461 tctgctgtca cccatcctgt tcctgtttct ctcaaaaaag ggggaacaga attttctatt
    23521 gttgcagaac aaataactgt tgtatcacca aaacggatca ctaccgatcc ttctggatat
    23581 ttgaaaacat ttgtttgaag ctccagtttg cgaagctctt gggcttttct cccgtcatga
    23641 cgcatcctta ttttcctccc aattcgatat gttctacatt taagtgtccg attccaagcc
    23701 agtcttcagc tatttcctcg aaaagtttgg cagagccagt tgtataaaac tggcataatg
    23761 tccgaccatt ttgcggtgaa ttgctcaaat tgaaataatc taacagcata gatacttcac
    23821 ctactgtttc tgctccagaa tcgatcagtt gaacattttc tcccattaca ttttgaatga
    23881 tggggcgtaa taatggataa tgggtgcatc ccaaaatcaa tgtatcgatt tttttagtgg
    23941 ttaaaggagc taatgtttct gccacaattt ttttcgccac cgatgaatgg tattcattac
    24001 tttcgacaac tgaaacaaat ttgggacaag caagacttgt cacagtcaat tctggtactt
    24061 tctctttcag tgctttttca taagcttgac tttttaccgt accaatcgta ccgataatgc
    24121 caacttgttt attttgtgtt ttttttactg ccgctctagt accgggaagg atcacaccga
    24181 tgactggaat agaaagagca gctttgattt cttctaaagc caccgcagtt gcggtattgc
    24241 aggcgatcac cagcatcttg attccttgct ccaccagata atccgtcatt tcccaagtat
    24301 actggattac ctgttccgcg ggtctagggc catatgggca gcgtgctgtg tctcctacaa
    24361 ataaaatatt ttcattcggt aattgtttca gggcttcttt tactacagtc aagccgccga
    24421 cacctgaatc aataaatccg atagggcgat tatctgtcaa tcgtatcatc ctttttttca
    24481 ataaattcta tcctccctat tgtgtacttt ttagcgtgaa ttttcaagtg ctcttacttg
    24541 cttaaaacaa tcgaaatctc tttttttcga atggaaagag gaaaagcggt tgtgacttta
    24601 gtttgccaca accgctaaaa gaatcatcta ttaataactg taccgtctct tttccaagta
    24661 accggaaagc agagaaagcg cgtaacatac cacgaagtac ataagtgtca tgatgatgaa
    24721 catcggaagc acataattgg tgttctgtcc ataaataata cgtgcctgat gagaaagttc
    24781 aggtaaagaa atgattactg ctaatgaagt atctttgatc agtgaaatca attggctcac
    24841 aattgccggg atcattgatt taaatgcttg cggtaacacg atcgttcgca tcgattctac
    24901 ataagtcagt cccgttgata atcctgcttc catctgtcct tttggaacag cattgagtcc
    24961 agctcggaaa atttccgaca acatagctga ctcaaaaatc gttaatgctg cgacagcgga
    25021 ccagaagatg ttcatctgaa tgccgatttg aggtaaggca aagtaagtga agaaaatgat
    25081 caataataat ggaagattcc gtataatatc aatcactaca ccaaggattt tggaaaagaa
    25141 agggatattc gcaaaacgaa tcacacctgt cagaccaccg atcaagaaac taaaaatgat
    25201 cgaaaacaat gaaacttcaa cagtaacctt taatccttcc aataaaaaac ggatattcat
    25261 ccatgaaaaa gcgccgctaa aatccatgta tttcccctcc ttctatgaac gaaccgccca
    25321 ttttttctca agataaacca ttaagtaaga taacggtagc gtgataacta agtaaaataa
    25381 cccaacgatg atatacgtat cgaaggtatt gaatgtttca ctagcgatca agtctccttg
    25441 atacatcaaa tctaaaccag ctacgattgc taaaatagat gagtttttta cgaggttgat
    25501 aaattggttg cctaatggcg ggataacgat tttgaatgct tgcggtaaaa caatataacg
    25561 catgatttct gaataggtaa aaccagaaga tagtcctgct tctgtttgac cttttggtac
    25621 ggtttgaatc cctgaacgta ctgtttcagc aataaaagca gatgtataga tcgttaatcc
    25681 aatcgtacct gcttggaatc catctagatt aaaccaataa atcggtacga caacataaaa
    25741 aaacatcacg atgattaata atggaatgtt tctaaaaaat tctacatatg cctttgctaa
    25801 ggcgctcact actttattct ttgataattg gaagattgcc attaacgttc cgataatcaa
    25861 actaaacaca agtgctaaga cactcgaaaa caatgtgatc ttgaacccgt cgacaaagat
    25921 tggtccataa gtttgtatca attcgctcat tgcctctccc ccttactcta cttttccttc
    25981 agtttcattt ggaaaccatt tttgatagat tttatcgtat gtgccatctg catgcatttc
    26041 ctctagcgct tggttaacag ctttcaagaa gttttcttgc cctttgttga tcgcaatccc
    26101 gtaaggctca ttagtaaacg ttccaccaac taactcatac tctggatttt catctgcaat
    26161 ccctaacaaa atcgcattat ccgtagtcat cgcgtctccc tgaccagatt gtaaagcagt
    26221 aaatgcttct gcatagtttt ctaattctaa aatttttgca tctggtgcat gctgtcggat
    26281 attcgcagcc gatgtagaac ctttgactgc tagaaccgtt gtagaagcat tcaaatcatc
    26341 tacacttttg atttggcttc ctttctttac caacaaagct tggccagcat cgaaatagac
    26401 atctgaaaag tcaacctgtt tttttcgttc atctgtgatc gtcatcgttg caataattgc
    26461 atcgatattt ccgtttttca ataatggaat ccgagtttta gaagtgactt caacaaattc
    26521 tgtttttcca ttgtcaccaa ggatcttttt agtgattgct tttgcaatat ctacatcaaa
    26581 tccttgtaca gttctgctct caatatccat cattccaaat aatcgagtat catacttgac
    26641 accccagatg atttcattgg tagatttgct tcgctctaaa atatcttcat ttgccaaccc
    26701 atttccttta catccggcta gtgcaagaaa agcaatgcct aaaaggaaaa tcaggcgtat
    26761 tttttttaac ctgcgcatct ttttcctcct atcaatgatt gataacttta ctgataaatt
    26821 gctgtgctcg gacttcattt ggattttcga aaaagtctct cacatttctg ctgtcttcca
    26881 atacttctcc atcagccatg aagataacac gatccgccac ttctttggca aagcccattt
    26941 catgtgtcac gacaatcatt gacataccgt catgagccaa ttttttcata acgtctaata
    27001 catcaccaat catttccgga tccaaagcag aagtaggttc atcaaacagc agcatctcag
    27061 gattcatcgc taaacctctg gcgattgcca cccgttgctg ttgtcccccg gataacatgg
    27121 aaggataaga atctttctta tctaacatat tgacagtttt caaaaatttc tccgcgtttt
    27181 tgactgcagt tgcctggtct tgcttcaaca ctttgattgg tgctaatttg atgttttcca
    27241 gaacagtttt atttgggtac aaattgaaat gttggaaaac catgccgatg ttttttcgga
    27301 cttctgtcag ttttgtcttt ttatcatgca agtcagtatc attaatcagc agcttacctg
    27361 aagagatcgt ctctaatcca ttgatacagc gaagcatcgt gctctttcct gaaccagaag
    27421 gaccaatgac aacgacaact tctcctttct cgaattcgag attgatattt tttaatgcgt
    27481 ggaacttgcc ataatattta tccacgtgat caaatttaat cattgccata gaattacact
    27541 cccattatta tttagtaaga tttcttttaa agatgtataa taaaatacat tatatctgat
    27601 agtttagtaa tttgtaaagt ttcttagaac gtttattctc ccataaatat ttcttccatt
    27661 tctaatctat tagttatttt atcctctttc cttgtttttt ccttaggaac tgttgtttct
    27721 tttcaggaaa aacggtaaga tattaagtgc tcagacttac aaatcgtaaa gaggtgtctt
    27781 ttttgaaaaa tattatttta acgggagatc gaccaactgg gaagctgcat ttagggcatt
    27841 acgtcggttc tctaaaaacc cgagtggcta tgcaggcaga tgaagacaac caactgtttg
    27901 tcatgattgc tgacatgcaa gcactgacag ataatgctaa aaatcctgag aaggtttcat
    27961 caaatgtctt gcaagttgct ttagactatt tagccgtggg attagatcct gcaaaatcta
    28021 ctttatttat ccaatcacaa attccggaat tagctgaatt aaccatgtat tatttgaact
    28081 tagtcagcgt aggacgcgtt cggcgcaatc cgactgtcaa aacagaaatc gaacaaaaaa
    28141 aattcggtga aagcgtgcct acaggattct ttatctatcc tgtttctcaa gcagcagata
    28201 tcactgcatt caaagcgaat cttgtacctg ttggtgagga tcaaaaacca atgctagagc
    28261 agacacagga aatcgtacaa agcttcaatc atacatatgg tgaagtcttg gtggaaccaa
    28321 aaggcgtatt cccaccaaaa ggaatgggta gattacctgg aatcgatgga aatggcaaaa
    28381 tgagtaaatc gttaggaaac ggtatttata tctccgatcc tgccgatgtc ttacagaaaa
    28441 aagtcatgag tatgtatact gatccaaacc acatccatgt acaagatcct ggacaagtag
    28501 aagggaatat ggtcttcacc tacttggatg tattcggaac agataaagaa gcaattgaag
    28561 aaatgaaagc tcattaccgc cgcggcggac ttggtgatgt aaagatcaaa cgatacctca
    28621 ttgatgtatt ggaagctgaa tttgcaccga tccgtgcgcg ccgagaagaa ttggaaaaag
    28681 atccttcagc tgttatggaa atcttacgta aaggtagtga agaagctgct aaagtagctg
    28741 cacaaacact ttctgaagta aaatcagcaa tgggaatcaa ttacttccat taattcattt
    28801 actctaaaaa agcaggctgt aaaggcctgt ttttttatct cgtctctgtg ctattccttt
    28861 tttaaaatca aaaaaagaga tgtgacgaag ttgtgtcaca tctttttttc aaatgtatgg
    28921 tcttcaaaag ctacttgatc gttacttact atattttcct tcttagcgaa tacttgcctc
    28981 taggttttct accaacgctt tttcgacttt ttccattgct ttattgattt cttcatctgt
    29041 caatgttgct tctgggttga cgaatgtcag gctatacgcc atggatttat gtcctttttc
    29101 aatgttttct ccttgataga catcaaataa ttggatatct gttaaaaatc ttccggcagc
    29161 agaacggata acttctgtta attcctgatt tgtcacagtt tcttttacaa gcaaagcgat
    29221 gtcccggctg actgctggga attttgtcac tgcttcgaaa acgatccctt tttgagcagc
    29281 ttccactata gcagataaat caaactcagc aacatatgtt tctggaatat cataactttt
    29341 tgtaatcgtt ggatgcactt gaccgacaaa accaataaat ttttcgccaa ggtaaatcgc
    29401 agcagttctt ccaggatgca tttccttcat ttctgtcact gtttgataat ggacatcttc
    29461 agtgatgcct agttgttcaa ataagttttc taaaagaccc ttgatggtga aaaagtctac
    29521 attttgagct ttcgtctgcc aatctttttc ttcccaaata ccagaaatag cgattgctgc
    29581 atgttttact tcttgcggca gatgtgtacg tggatcattt tcttggaaga acacccgacc
    29641 aatttcatat aaagcaatct ctgtattttt tcgggcagtg ttataactga tatcatctaa
    29701 tagtccgctg atcaaattca ttcgtaatac agaacgttct tcgctcattg gccagtccaa
    29761 acgtgtaata ttactttcat ttataacaaa ctgtcttgat ttttcttctg ttgtcaatgc
    29821 ataactgatt gcttcgctca atccgcttcc ttcaaagatt gtacgaattt tacgagtagt
    29881 ttcttgttct tttgacaagc ttccagcaac cgtttctcca cttggaagtg tagatggcaa
    29941 ctggtcgtat ccataaatac gagcaacttc ttcgataata tctgcttcga tttggatatc
    30001 ccagcgtctt ggtggtacta ctacggaata aacatactcc ttttcttcat agctaaatcc
    30061 aagtgcttca aaaatctcat ttacttcttt aacgacaaga gatgttccta aatattgatt
    30121 gatgcgttca agtgaaatct tcactgatac gtcttctgct ttgatctctg agcctttgac
    30181 cgcgccttgt agaacttccc cacccgcaag agaaacgatc attgctgcag caacctcact
    30241 ggcttctcca acagtcgcat ggttgattcc tttttcaaaa cgtgcagagg attcactacg
    30301 caaattaaat tgttttgctg tacgacgaat cgaaattgga tcaaataaag cagattctag
    30361 tgcaactgtt gttgtttcat tcgtgatctc tgagtctaac cctcccatga caccagctaa
    30421 agcaactggt tctttgccgt tagtaatgac gagattgtca gttgtcaatt cccgagattc
    30481 tccatctagt gttactaatg tttcattttc ttttgcacgg cggacaaaga tctcttttga
    30541 tttcaatttg tcgtagtcaa atgcatgtag tggttgtccg aataaaagca aaatatagtt
    30601 cgtcacatct actacattgt tgattggacg aatgccttcg ttcatcaaaa gattttgcag
    30661 ccattgagga cttggctgga ttcttactcc ttcaatgatt ctgatttgat aagcaggaac
    30721 tgcttcttta tcttcaactt ccacttgaat tttatcgcta gcaggacgtg aggattctac
    30781 cagcttttcg tcatcgaatg taggagtctg acgataaatt gccccaactt cataagctac
    30841 gccacgcatt gacaatgcat ccgcacgatt cggtgtgata gataattcaa taatcgaatc
    30901 atccatatcc aaataaggaa aaactggatc gccatttaca gcttcttgtg gcatataata
    30961 gataccttca gcgtattctt tcggtataac actatctgaa tagccaattt cttgtaatga
    31021 gcaaatcatg ccatttgata cttggccacg catttttcct tttttgattt ttacattacc
    31081 agcgatacgt gagttaggaa gtgcaacaat cactttgatt ccaaccttca cattaggcgc
    31141 tccacaaacg atttgagaca gttcgtcttc gccaatatcg acttgacaaa tagaaaggtg
    31201 atcagaatct ggatgtggta cgcattcctt gacttcaccg acaacgattt ttttcaagcc
    31261 ttcttctgga accgttactc cctctacttc gattcctgtc acagacatct tatccgctaa
    31321 ttcttttggt gttacattcg ataaatctac ataacgattg agccatttat atgaaactaa
    31381 cattttctta ctcccccttg aactgactta agaaacgtaa gtcattttga tagaaattac
    31441 ggatatcatt cactccatac cgcaacatag ctacgcggtc tggtccaagg ccgaaagcaa
    31501 agcctgaata ttcttctgga tcgattcctg acatcttcaa cacattcggg tgtaccatgc
    31561 cagctcctaa aatttcgatc catccagtgt atttacatac gttacaaccg gcaccaccac
    31621 atttgaaaca gctgacatct acttctactg atggttccgt aaatgggaaa taacttggac
    31681 gcaaacgaat ttcacgatct tccccaaaca tttttttcat tacgacttct aatgttcctt
    31741 taaggtcgcc cattgtgatg tttttatcaa caactagccc ttcaatttga tggaactgat
    31801 gactgtgggt cgcatcatct gtatctctgc ggaagacttt ccctggcgag atcattcgta
    31861 gagcaccctt agagaaatca tgtttttcca ttgtccgtgc ttggactggt gaagtatgtg
    31921 tccgaatcaa tatctcgtct gaaatataga aagtatcttg catgtccctt gctggatgat
    31981 cttttggcag attcatccgt tcgaaattgt aatgatcaga ttctacttca tatccttcta
    32041 cgatctgata gcccataccc acaaaaatat cttcaatttc ttccatgatt tgtgtcacga
    32101 tatgacgtgt accatgtttt acttttctac caggtagtgt cacgtctatt gtttcttttg
    32161 ccaaagctgc atttaacgct tcttcttcta atacttgttt ttttcgcacg atctcttctg
    32221 tcaaaagatc tcggatctca tttgcgaaac ttccgacttt tggtcgctct tctggactta
    32281 gatctttcat cccacgtaat acttctgtga tcggtccttt ttttcctaac gtttcaacac
    32341 gtatttgatt taattcattt aaatcttgtg ccgctgtgat tttgacgagc gttgcttctt
    32401 tcaagctctc taattgtgct tgtagagaca tgtgcttgtt cctcctttaa attccaaata
    32461 caaaacaaaa agacctcatt cctgttaagg aacgaggtct tcgcgttacc atcctaattt
    32521 gcggataaat gacttacacc gcactcttac ttcgttaacg acaattgccg gtctgatttt
    32581 atcagacgaa ctcggaaagt gaacttcatt tttcaaacac aggatctgct ttcagtctgc
    32641 gacagatctc cctgaactgg tttagccaaa ctactctttt tcgtcatcgt tctttactta
    32701 ttcgtatgga ctatagttta caagattttt tttacaaaat caaccgcatt cttcctctgt
    32761 gatccatttt tcgctccaaa cttgtatttg acgcatcgct ttttgcaagt ctgctccttt
    32821 ttcagtcaag agatattctg aacggctctt ttcgctacag gcaaagtttc gagtgactat
    32881 tccttctttt tctaattcct tcaagcgttc gactaatact cgatcactga ccatcgggat
    32941 ttttgccgca agctcaacaa agcgttgcgg accgttttcg agtagtacat cgataatcaa
    33001 tccgttccat ttttttccta aaatagagaa tgttttttca aatttagggc ataaaacgaa
    33061 ttctgtctgt gttagctcca tcatactcac ctcttcccag agtatagcat aatccttaca
    33121 aaacataagt caagtttgct caattataat tatttgttaa tgtttataat ttgtttcact
    33181 cgaagcaaaa ttaggaaaac attttctact ggcttattca aagcttctga cattgcttgt
    33241 tggtaaagtc ttagctgtcc ttgatatttt tggacgattg catctatttc tgctggatca
    33301 tccggatgta aaataaaatc tgttttaaaa tcatagatat tgagatgatc tttttcttct
    33361 aggtatccat cgacgattcc gtggattaat agttcatctt cttgatttgg atagtcttga
    33421 aatacttcgt cagcaggaag cagcatggaa aaaggttgtt ctcgttttac gttttctttg
    33481 ttggcaatca gctgttgacc aagttctgtt tgaaagaacc atataataga agaaacatct
    33541 acttttttcg cgactttttc gtctactaac cgtttcctta ccaattcctg taatttttga
    33601 tggatactct ctgtagttgg catctctaat ggcagtaatt gtaacaaagc atgcgtagca
    33661 gttccaacag cagctgctga cacttgccga tctttttgca aaaattttgg ttctgctagt
    33721 tgttcttgcg tataacgaaa ttgacgggat gctgctttgt tctgactgct ttccagagtc
    33781 aaacgtgatt cttgtgtatc atctggatct tcgaataatc gtttgatttc agatacagat
    33841 tgataacttg tggtttgtga agaagcttga taaggatatt catatgccag acgtttttgt
    33901 aacgaatctg ctaataaagt ttcatcttct ttcatctgct gatctaagtt aaccagttct
    33961 ttttcagcaa gcaactgtct ttgttcaatg atcgattgct gattcatcca tgaaatagaa
    34021 aactgtgcat ttgaatgttg aagaagagaa gtcgaaattt cttcctctaa gtatttctgc
    34081 atttcaggat gacggatcaa tccgtaacca atccaattca tcaagctgct tctgcccttc
    34141 aaacgtagcg caggatcaaa aactaactcc tcatgatcag ctgcttcaga ccacgtttgg
    34201 atcatttgct ctttattttt ataggaaccg acaatgaaaa gcttttgttc tgcccgagtc
    34261 aatcctacat acaatttacg catttcttcc gacaacaatt tgttttgctt tgccaatttg
    34321 atggcttgaa acggtaacgt atcatataac actcgtgttt caggatccat atagcgaatt
    34381 cctgctccaa gtttttcttc aaaagcatac cgattgcgca agtcttgtaa gttgaactcc
    34441 tttgtcatat ctaacaaaaa tacaactggg aattccaagc ctttacttgc atggatcgtc
    34501 ataacgcgaa cagcattatc ttctattgaa atgactggtt ctgctaagtc tttatctttt
    34561 tcttgcattt tttcaatgaa acgcacgaac tggtataatc ctctgaaaga actttgctcg
    34621 taagctttgg cacgatcaac caatgcataa agattggctt gtctctgtgc ccctgctggc
    34681 agaccgacca catactctag atatcccgtc tcataataga tatcccataa caagtcagtg
    34741 atcgaagaac ggcgagcaag ttctcgccaa tgctctagtt gcttgccaaa atgttccagc
    34801 ttagctgcta attcatcttc gtttgatgct tgataagcaa gcacagcatc atagtaagta
    34861 tgtgcacggt tagctaatcg aatgctggct aattctggtt caatcagacc cacgatcggt
    34921 gagcgcaata cagccgcaag cggaatatct tgatagggat tatcgattaa ttgtagcagc
    34981 gagatcatcg tgcgaatttc tgtcgcttga aaatagtttt gagcatcatt catctctaat
    35041 ggaatatcca atgttttgaa aatctctaaa atcgttaagt tgttcttctt tgtaggtgtc
    35101 aagagaacaa tgtctttgta ctctatcgga cgactttttt ttgatttttt atcataaatc
    35161 ataaaggagg aatcaattaa ttgacgaatt tttagtcctg tcataaaaag ttcgccttct
    35221 gttttgtctt ccagtatatc atccgtcgga atctcaatct ctgattcttc ctgttctttt
    35281 tcatagatca tgatttccgg ctcaaactgt tcattctctg gaaagtctga aaatccttgg
    35341 atcagtttgg ctgcttcgtc ataattgatt tgacccactg ctggatccat tagttgttca
    35401 aaaataagat tcgtaaaaga aaggacttct tttctagagc ggaagttctc agccaaaacg
    35461 attcgacgtc cgccttcttt ctttgagaaa ttctcgtatt tcccgataaa taaactagga
    35521 tctgctaaac ggaaagaata gatggattgt ttgacatccc caaccatgaa catatttcct
    35581 tttgtatcat cgggttctct tagccagtat aaaatggctt cttgtaattg gttcacgtct
    35641 tgatattcat ctaccattac ttctttgaat ttttttcgat aatgttttga agcttcactt
    35701 ggcagccata catctttcgt tttttctgtg agaatctgca acgctaaatg ttccagatca
    35761 ttgaaatcca gcacaccttt ttcccgttta cgcatactga aaccgtccat gaagctttgc
    35821 gtgacttttg tcatttcttc gactaacggt agtgctttgt ccgttaattc ttccatttgg
    35881 cttggggaaa caggaaaata acttttactt atctgctgaa tcagtttttt tgctttatcc
    35941 ctcatgccct tgacttcatt ggaaatttct ttcaattctg ctttccgact gctcttaaaa
    36001 gttgaaaaag ttagttgttc caaaccagca taagcttctt ctaaccgatc ttgcgagaac
    36061 gcttcataga tattcttgat ctgctgttgt tctccagcta cttgctcatg cattttttct
    36121 aatccttcac cttgtgctaa ctgagtcatc tgttcatata actgtacaca ttggtatata
    36181 tctgccaaaa ctaaaggacg gatttgtttt tgatataatc gagatttcgc tagcccctca
    36241 ggcaaacgat aattatcgct taactgttct agccattttt gggggtcagg gttggcacgg
    36301 gcaaactcat aaagagaaaa caccagattc gtcaagccgt catctgaacg atcattagaa
    36361 aaattcattg ttaattggaa aaatcgttca tcattttctg cataaagtgc ttctctcagc
    36421 tcgtcccaca catcctcctt catcaagatc gtttctgttt catctgttaa catgcgaaag
    36481 actggatcaa tatcaattaa gtaataataa cgtcgaataa ctgtcaggca aaaagcatgt
    36541 aaggttgaga tattcgctgt gggcaataaa actaactgct tagtaaaatg ctgtctacgt
    36601 acagggtcac tttcgctatt gacggattct tgtaatgcct cttgaattct ttctttcatt
    36661 tctcttgcgg cagcttcagt aaatgtcaca atcaataatt catcgatatc aaaacccatt
    36721 tttagttttt caatcactct tcgaacaagc actgtagttt tacctgatcc ggcagacgca
    36781 gagactaata gattatctcc ttgatcaaaa atcgcttgcc actgatcatc tgtgaatcgt
    36841 tcattttgcg gttttaaagg aatttgattc actcttcttc actccttttc aatattctac
    36901 gcagtgcttc ttccttatct attttctcca gacggtggta attattttct ttcagcatca
    36961 catcaaacgt acagatgctt ctgaatggac aaaattcgca ggcaatccgc tgtttatctt
    37021 tataagctgg atttagtttg atttctccgg aagtaatcgc atctccagct aaccgcatat
    37081 tttcccgatt atgcgccatg aatagttgca gttcttcttc cgtgaaaaat ttgtctttac
    37141 tgaaactacc tttttggtat tcatctttcg catttttttt cactggatag atcagtgatt
    37201 gttcttttgc ttccagtgaa tgatctagta cttctaaaag agcgggatca tcgacaaaaa
    37261 cgccatcata actaaatttt ttcaatgtat tcttctctac atcatctgtc tccgtcaata
    37321 caggattatg aacatgtaaa taatatgatc ctgctggttt aacagccgcc tttccagtca
    37381 aacgtaccgc atccatcaat gctacgtcca gatacgtcaa taattgcata gccatgccat
    37441 aataagcttc tgtgacatcg aatgaacgtc cgctagattt gtaatcaacg acgcttagcc
    37501 aagtatcttc ttgttcagaa gctacatcga ttcgatcgat tttccctcga acatggagtt
    37561 ttccaccatt actcaacggt aattccagac ctgtgatacc ttttgctcca gcaatctgac
    37621 caaatagaat ctctgtctga agaggatgca tgcctgtatt tttgctctgt ttttgcaaag
    37681 cccatgtcac tttttgaatc gtctgtccta actggtagcg gatatagttc attcttgcag
    37741 aactatccaa tatctcaaac cgaagttctc cgaatacttc ctgcagcact ttttcagtaa
    37801 agtctctacg ctgcgcatcg gacatattca ctaaggataa ttggtttgaa aacagtagct
    37861 tgaagaaacg atccaatgat tcatgataaa aatcaccagt tgctgctgga gttagaccat
    37921 aaacagttcg ttctttcaat ttcaaaccaa attgcatgaa gtatttgtat tcacaattat
    37981 aaaaattctc catacgtgaa attgaagtat aaatctcttt tccataaagc tcttccgcca
    38041 ttttttctgg catcgaaaca ggaacattta aatgttcctg actttcaaaa atctgtaaag
    38101 caaaatttcg ccatgacgaa ttcagtacta attctttgag cttttgccag tgctttggta
    38161 caggtttttt ttcatctaaa gctaatcgat aaatcctatt gactgtattg attccgcttc
    38221 tgtaagtccc tacataacaa ttagggtcgc tttcgatcgt caaatgctct tttctctcga
    38281 cgggaacaga taaatattct gtgattctac gcaaataagg agacatcttc agcgaaccat
    38341 ctgtgtcata gctttgtgca taactaagat ataatcgatc tgttccggaa agaaaaacaa
    38401 gataggcttg gaaaggctca aaagagatac tttcctttga tgggtcgaat aaaaactgtc
    38461 catctggcaa ggcctgattt aatacttctc gttcttcact ggacaacaat ccttttgtct
    38521 cttttcggtc tggaaacact tgatcattca accctaatgc aaaagtgacg gcagcttgat
    38581 ttggtcgaac taattccaaa cgattgattc gcacttggtc gattgcggta gggattttac
    38641 cataacttag attttctaga ccacttgtaa tgatttcttg gaacagtgac cagtcaaaag
    38701 ccgattcacc ataaattact acatattcat ccaacagatc aatcagcgct ccccatgtct
    38761 gctcatggtt tcttgccgct tctaagtttc ctctgtctac ttcctgatta cgccaaaaca
    38821 ataattgttg ctgtacgcca ctttggatca aaaattgata aaaaacattt gcggcctctt
    38881 gtcctgtttt gcttttcttt atttttttca aaaatactgg tacagctttc tgaaataatc
    38941 gtctaatcgt atttgactgt tcttccaaat actgtacgtc ttcaatctgt tctgcttcaa
    39001 aatcatacgc cacaaactgc cagtcttttt cacgagtcca ataagttcct tgataattgt
    39061 aagcaagtgc tacattttca gtgatgtcca gttttttcct gaattcatct cgctcttgtt
    39121 tccattcatc aagttcggtt tctgcttccg tcgacagaaa taattcagtt ttgaaaaaac
    39181 gcataatatc acttagccga taagaatagc ggtcaagagc gaacagactg ttgatccatt
    39241 cgatcagcgg atgattttcc atcatttgtt cttcatcgat ataaaaagga attgccattt
    39301 cttgaaagat cggccgaagg atatttccat ataaagacat attttttgtc agcacttgga
    39361 tatcacgata acggtagttt tcttcactga ctagtcgtct gatttcaaca gctaattgtc
    39421 ggacttcttc agttggattt tcagcttccc acaaatggag atgctcttgt ccccctattt
    39481 tgtgtactgc cgctttcttt tggctagatg tctcttccca cattgtttgc agcagttgaa
    39541 gctctgtcgg acgagtctgc tctggtgcaa atcggtccac taatataggg actttccgtt
    39601 ctcttgcact ttgaaaaaga cggaaataaa gctttccagt ctcatgaaag agcgtaagta
    39661 cttctggaag atccgctgta taaggacgat ccagcaataa atcgacaacc aattgcccct
    39721 tttccatcat tgcctgcagt aattgatact cttgaccatt gattcgagaa aaaccactga
    39781 caataaattt gacgttgttg aaatcttgtg tctgcatata atttgctagt atagatagtg
    39841 catcttcatt tgttagtgca cgttgagtca attcgctgtc ataagctgaa aaaatcagct
    39901 tcaagtcttt cattttaagt tgctggtctt ctgcattagg tgaatcacca aataaaagca
    39961 tcaggtcttc tggttgaata tttcctgtac gcaattcact gtagagttct tgtaattgac
    40021 gaataaaccc tcttttattg atttctccgc gaaagattgt taattcttcc tcattttttt
    40081 ctaaaatctg tcgcaagatc attgcagatc ctgattctga taattcattt cctgtaagaa
    40141 gagttgtctg ttgcagcaaa taccaagcca gacgataaaa actaaatact tgaacgtttg
    40201 ttgtgctgaa agtttttttt ccgcttcttc ttcgcatctc cgctaaaatt tcttgttcct
    40261 gttcgaattt gctgtagttg ggaacaagga aaaatacttg gttacttgca tcctggtcga
    40321 gccaagcgga tgcttcgtcg atcaagactt gacggtgatc ttttttcccg tttccaagaa
    40381 taaattgtaa agacatgctt ttcctccttc aacacatgat acggtgctcc tttattatat
    40441 caaaaaatac tgaacatttg ttctatattc ttcaaattat atccttttaa ggttctgata
    40501 tttctacttt tttaagtata cgcaaaaa
//
LOCUS       DACDEA010000021        39950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDEA010000021 DACDEA010000000
VERSION     DACDEA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.021"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(142..990)
                     /locus_tag="IV136_001094"
     CDS             complement(142..990)
                     /locus_tag="IV136_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8465100.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1214..3154)
                     /gene="thrS"
                     /locus_tag="IV136_001095"
     CDS             complement(1214..3154)
                     /gene="thrS"
                     /locus_tag="IV136_001095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8465101.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3491..3670)
                     /locus_tag="IV136_001096"
     CDS             complement(3491..3670)
                     /locus_tag="IV136_001096"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8465102.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3717..5540)
                     /gene="cadA"
                     /locus_tag="IV136_001097"
     CDS             complement(3717..5540)
                     /gene="cadA"
                     /locus_tag="IV136_001097"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8465103.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5818..8097
                     /locus_tag="IV136_001098"
     CDS             5818..8097
                     /locus_tag="IV136_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8465104.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8190..8837)
                     /locus_tag="IV136_001099"
     CDS             complement(8190..8837)
                     /locus_tag="IV136_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465105.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9016..9735)
                     /locus_tag="IV136_001100"
     CDS             complement(9016..9735)
                     /locus_tag="IV136_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8465106.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9866..11017)
                     /locus_tag="IV136_001101"
     CDS             complement(9866..11017)
                     /locus_tag="IV136_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8465107.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11234..11974)
                     /locus_tag="IV136_001102"
     CDS             complement(11234..11974)
                     /locus_tag="IV136_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8465108.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11978..12301)
                     /gene="rsfS"
                     /locus_tag="IV136_001103"
     CDS             complement(11978..12301)
                     /gene="rsfS"
                     /locus_tag="IV136_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8465109.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12358..12951)
                     /locus_tag="IV136_001104"
     CDS             complement(12358..12951)
                     /locus_tag="IV136_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8465110.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12935..13582)
                     /locus_tag="IV136_001105"
     CDS             complement(12935..13582)
                     /locus_tag="IV136_001105"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8465111.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13595..13906)
                     /gene="yhbY"
                     /locus_tag="IV136_001106"
     CDS             complement(13595..13906)
                     /gene="yhbY"
                     /locus_tag="IV136_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8465112.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13922..15031)
                     /gene="yqeH"
                     /locus_tag="IV136_001107"
     CDS             complement(13922..15031)
                     /gene="yqeH"
                     /locus_tag="IV136_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8465113.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15028..15555)
                     /locus_tag="IV136_001108"
     CDS             complement(15028..15555)
                     /locus_tag="IV136_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8465114.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15735..16520)
                     /locus_tag="IV136_001109"
     CDS             complement(15735..16520)
                     /locus_tag="IV136_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8465115.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16513..17382)
                     /locus_tag="IV136_001110"
     CDS             complement(16513..17382)
                     /locus_tag="IV136_001110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8465116.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17383..18762)
                     /locus_tag="IV136_001111"
     CDS             complement(17383..18762)
                     /locus_tag="IV136_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8465117.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18770..19189)
                     /gene="fabZ"
                     /locus_tag="IV136_001112"
     CDS             complement(18770..19189)
                     /gene="fabZ"
                     /locus_tag="IV136_001112"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8465118.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19214..19690)
                     /gene="accB"
                     /locus_tag="IV136_001113"
     CDS             complement(19214..19690)
                     /gene="accB"
                     /locus_tag="IV136_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8465119.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19693..20928)
                     /gene="fabF"
                     /locus_tag="IV136_001114"
     CDS             complement(19693..20928)
                     /gene="fabF"
                     /locus_tag="IV136_001114"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8465120.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20947..21684)
                     /gene="fabG"
                     /locus_tag="IV136_001115"
     CDS             complement(20947..21684)
                     /gene="fabG"
                     /locus_tag="IV136_001115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8465121.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21696..22613)
                     /gene="fabD"
                     /locus_tag="IV136_001116"
     CDS             complement(21696..22613)
                     /gene="fabD"
                     /locus_tag="IV136_001116"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8465122.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22720..22944)
                     /locus_tag="IV136_001117"
     CDS             complement(22720..22944)
                     /locus_tag="IV136_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8465123.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22997..23959)
                     /locus_tag="IV136_001118"
     CDS             complement(22997..23959)
                     /locus_tag="IV136_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8465124.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23969..24409)
                     /locus_tag="IV136_001119"
     CDS             complement(23969..24409)
                     /locus_tag="IV136_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8465125.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24715..25680
                     /locus_tag="IV136_001120"
     CDS             24715..25680
                     /locus_tag="IV136_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8465126.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25737..25967)
                     /locus_tag="IV136_001121"
     CDS             complement(25737..25967)
                     /locus_tag="IV136_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8465127.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26053..26940)
                     /locus_tag="IV136_001122"
     CDS             complement(26053..26940)
                     /locus_tag="IV136_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8465128.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27029..28252)
                     /locus_tag="IV136_001123"
     CDS             complement(27029..28252)
                     /locus_tag="IV136_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8465129.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28269..29348)
                     /locus_tag="IV136_001124"
     CDS             complement(28269..29348)
                     /locus_tag="IV136_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8465130.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIEKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29479..31206)
                     /gene="ptsP"
                     /locus_tag="IV136_001125"
     CDS             complement(29479..31206)
                     /gene="ptsP"
                     /locus_tag="IV136_001125"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8465131.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31206..31472)
                     /locus_tag="IV136_001126"
     CDS             complement(31206..31472)
                     /locus_tag="IV136_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8465132.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31951..34185
                     /locus_tag="IV136_001127"
     CDS             31951..34185
                     /locus_tag="IV136_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8465133.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34333..34653
                     /locus_tag="IV136_001128"
     CDS             34333..34653
                     /locus_tag="IV136_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8465134.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34858..35082)
                     /locus_tag="IV136_001129"
     CDS             complement(34858..35082)
                     /locus_tag="IV136_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8465135.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35280..36860)
                     /locus_tag="IV136_001130"
     CDS             complement(35280..36860)
                     /locus_tag="IV136_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8465136.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37261..38637)
                     /locus_tag="IV136_001131"
     CDS             complement(37261..38637)
                     /locus_tag="IV136_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8465137.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38765..39883)
                     /locus_tag="IV136_001132"
     CDS             complement(38765..39883)
                     /locus_tag="IV136_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8465138.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11345 a         8583 c         6895 g        13127 t
ORIGIN      
        1 caactatttt tttcattaaa aaataaactt atttttgaaa attatgtttt cttaagtcgt
       61 gaaacacttt gatggatcat ttatttgtgg aaaactacgt tttgttattc taaaaaactt
      121 atataaaaaa tagtaaggtt tttatttcga atccataaaa tattcttgga ttgcttctcg
      181 tgtttttttc actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat
      241 atcgatcgta gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc
      301 ttttggtcta gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa
      361 ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac
      421 atctgcactt ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac
      481 aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga
      541 ccatttttgc agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac
      601 agtctctgcc gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc
      661 tactgtttct tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa
      721 ggactcattt gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga
      781 aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac
      841 tgtcggcgca ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt
      901 ctcaatatta ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat
      961 catactttca atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta
     1021 tgtaagtata ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa
     1081 caatccgccc aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg
     1141 tctttgagga atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca
     1201 ggaaatttac ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa
     1261 taaagatatt caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg
     1321 ttgcatcttc caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag
     1381 aggcacgtat cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct
     1441 tcatttgcaa acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag
     1501 ggatgattgt tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg
     1561 ttaaataagc gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac
     1621 gatgcgtatt ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga
     1681 agtctaattg tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat
     1741 ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt
     1801 catccatagc ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat
     1861 atttatctgt attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga
     1921 agtcagcata tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat
     1981 caggacgaac aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg
     2041 acaaagcacc tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta
     2101 attcacgata agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg
     2161 ggcgaagaac aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat
     2221 aatgatccca gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata
     2281 catgttggta acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga
     2341 ttgttgcccc ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga
     2401 acaagtcaag ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac
     2461 ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca
     2521 tttgattgtt tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt
     2581 gtatacggcc agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt
     2641 gataagcagt aatcacttct tcttctggca attcactaat cagttctact ttgtacggat
     2701 cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat
     2761 tttctttgac gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt
     2821 ggttttgtcc attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa
     2881 agtggatatt tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac
     2941 ggactaagcc aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct
     3001 cgattggacg agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg
     3061 ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt
     3121 tgactgcacc atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg
     3181 attgatcaga tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac
     3241 taggacgaaa agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt
     3301 gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt
     3361 ctcctggaaa agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg
     3421 gctttctcat caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg
     3481 gaaactaaac ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat
     3541 tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga
     3601 tatctttcat catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta
     3661 cgaatggcat aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat
     3721 actatgtttg tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag
     3781 gcactcaaaa tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg
     3841 attagtcccg tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca
     3901 gactgtttcg ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag
     3961 agatcatctt tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc
     4021 cccattgcaa taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct
     4081 accatcgcaa caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga
     4141 ggcaagctac gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg
     4201 gcagatccat ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg
     4261 attgtctttg ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat
     4321 cgatcaattg ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa
     4381 gaggcacgtc caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc
     4441 acttctttca gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa
     4501 cgagctaaga tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct
     4561 ttttttattc cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt
     4621 cctgttttat caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt
     4681 tttacaacga tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca
     4741 gctagaatca gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct
     4801 tttgagaacc accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca
     4861 taacgatcag ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta
     4921 ataatcattt gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg
     4981 tctccattta aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac
     5041 tcacctgtca aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg
     5101 ggtacaagtt cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact
     5161 atatcctgca attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa
     5221 gactgcagtt ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta
     5281 agcatgatca atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt
     5341 gcggtaatcg ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc
     5401 aaaaacatca agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc
     5461 ggctggtgaa aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa
     5521 aattttttga aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta
     5581 ataaatttcc agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac
     5641 aataggcctt aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac
     5701 aaacaaaaat attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt
     5761 cgaaaaacta tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg
     5821 aatgagaaac aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa
     5881 caaaatatgc tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa
     5941 gacacagcta atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt
     6001 gaatatcgcc aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa
     6061 aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt
     6121 aaagaagacc gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca
     6181 gaagaaacaa tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag
     6241 attggtcccg cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa
     6301 cgacaattta aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc
     6361 aatgatgact ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc
     6421 gtctctacga tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg
     6481 ctgattgaag gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt
     6541 ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac
     6601 catttgtttt ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct
     6661 acacagacat tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa
     6721 cagcaacaag aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc
     6781 ggattgacct tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc
     6841 ttgttccgtg atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa
     6901 tggtataaag aaacaaatga acaattacct ttacatcata gactttctct attacaaacg
     6961 aagctcctga aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa
     7021 gatctggcgg aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca
     7081 gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc
     7141 cgtcgaatcc atcgagggat catgaattat caatttgtca atataacgaa acaatatctc
     7201 cactttctac aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg
     7261 tggcaggaac atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag
     7321 gatgctgtac tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa
     7381 gctcgctata tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg
     7441 caagccttgt tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt
     7501 ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt
     7561 ttccaattaa cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt
     7621 attgtgaaag aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc
     7681 caaggaacaa cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag
     7741 ggaaaagccc ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct
     7801 ctttatgctc aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt
     7861 gactttatga agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt
     7921 gaccgagttt tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg
     7981 atcctttaca ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat
     8041 gaaagtcctt tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta
     8101 aaaagacaac tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca
     8161 gaaaaagtaa ttcaatcatt taaggtcatt tattctatat ataaatccaa atctgtttca
     8221 ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt ttctggatat
     8281 tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa tcggtaaatt
     8341 cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata gccgccacaa
     8401 tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacttt attttttttc
     8461 gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgttt caagctccac
     8521 tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg aaaggctgaa
     8581 tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag cattggtgcg
     8641 cggtccacca tacactctaa ataacggttc agataccctg taccgacata gtttcctaat
     8701 cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca atcagttacg
     8761 ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa
     8821 gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata tcatttcaca
     8881 tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa atcacacaaa
     8941 aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc
     9001 gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc
     9061 tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt
     9121 tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc
     9181 ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc
     9241 ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc
     9301 gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc
     9361 tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt
     9421 tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc
     9481 ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg
     9541 gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt
     9601 ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa
     9661 ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc
     9721 ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac
     9781 gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc
     9841 attcttctca tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt
     9901 ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt
     9961 gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc
    10021 ttcttccagc atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa
    10081 tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg
    10141 tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg
    10201 ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca
    10261 aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg
    10321 aacaagttga aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt
    10381 cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt
    10441 catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg
    10501 cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa
    10561 tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat
    10621 cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga
    10681 aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca
    10741 gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga accgcccatt
    10801 cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc
    10861 atttatcgat gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga
    10921 cgacctctgc tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa
    10981 aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc
    11041 gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta
    11101 tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat
    11161 gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga
    11221 gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg
    11281 ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca
    11341 ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa
    11401 atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt
    11461 tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc
    11521 tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc
    11581 cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt
    11641 tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata
    11701 aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc
    11761 tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg
    11821 caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt
    11881 cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt
    11941 catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat
    12001 cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat
    12061 ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc
    12121 cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa
    12181 ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg
    12241 aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa
    12301 tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta
    12361 tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg
    12421 cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc
    12481 ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc
    12541 tgctacataa atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg
    12601 gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc
    12661 aacaagacca tgccagattg cattattata attcaataac tccggatcaa aaccgtctct
    12721 tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc
    12781 agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc
    12841 ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat
    12901 caggttttct ctagaataag cagtatattc attactatag tccatattca tacaaccctt
    12961 cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt
    13021 tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta
    13081 caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag
    13141 aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa
    13201 taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt
    13261 aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca
    13321 ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat
    13381 gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt
    13441 gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta
    13501 cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa
    13561 atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata
    13621 gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt
    13681 ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat
    13741 ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt
    13801 gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat
    13861 gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc
    13921 cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta
    13981 ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt
    14041 tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag
    14101 gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc
    14161 gatgaatatt tacatcattt gacacataag caataaatga agcacgattg ccgctgataa
    14221 aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt
    14281 taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg
    14341 ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag
    14401 gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta
    14461 ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc
    14521 cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca
    14581 tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt
    14641 aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat
    14701 ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga
    14761 ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc
    14821 ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tggatctcat
    14881 tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt
    14941 ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac
    15001 cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg
    15061 gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt
    15121 ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc
    15181 tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc
    15241 caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata
    15301 caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac
    15361 aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc
    15421 atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc
    15481 tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt
    15541 gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc
    15601 ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt
    15661 tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta
    15721 gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc
    15781 tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg
    15841 actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt
    15901 cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga
    15961 ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt
    16021 tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct
    16081 tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg
    16141 gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta
    16201 ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct
    16261 tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc
    16321 cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca
    16381 acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag
    16441 atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca
    16501 tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt
    16561 atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa
    16621 taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac
    16681 gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat
    16741 tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag
    16801 accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa
    16861 gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc
    16921 ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc
    16981 catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt
    17041 accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt
    17101 atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact
    17161 tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca
    17221 atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg
    17281 acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt
    17341 aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc
    17401 cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc
    17461 tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc
    17521 ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt
    17581 tccatggacg attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg
    17641 gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat
    17701 cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat
    17761 tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat
    17821 ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac
    17881 ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc
    17941 gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag
    18001 tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt
    18061 gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc
    18121 taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc
    18181 atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc
    18241 ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac
    18301 tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt
    18361 acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt
    18421 tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca
    18481 catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc
    18541 ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt
    18601 tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc
    18661 ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac
    18721 agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca
    18781 taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta
    18841 ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta tctcccggtg
    18901 taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga
    18961 attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata
    19021 cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta
    19081 catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa
    19141 gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc
    19201 tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg
    19261 actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca
    19321 tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt
    19381 ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa
    19441 caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg
    19501 ttgcttgttc agatatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa
    19561 ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac
    19621 gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat
    19681 tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa
    19741 ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac
    19801 tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg
    19861 ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc
    19921 agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg
    19981 gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata
    20041 tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc
    20101 accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc
    20161 aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac
    20221 tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga
    20281 accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc
    20341 catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt
    20401 gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc
    20461 tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat
    20521 cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac
    20581 ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc
    20641 ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat
    20701 tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta
    20761 agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt
    20821 gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt
    20881 tccaataggt gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc
    20941 cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata
    21001 tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg
    21061 catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc
    21121 agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg
    21181 tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc
    21241 aatatttcca atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt
    21301 catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag
    21361 acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc
    21421 attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc
    21481 cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc
    21541 gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc
    21601 agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa
    21661 tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca
    21721 aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat
    21781 ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac
    21841 ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg
    21901 attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca
    21961 gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct
    22021 gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat
    22081 agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta
    22141 ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc
    22201 gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg ggtagcttct
    22261 gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta
    22321 ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt
    22381 ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta
    22441 tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg
    22501 agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt
    22561 cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc
    22621 tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta
    22681 tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac
    22741 gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa
    22801 ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc
    22861 ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat
    22921 ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt
    22981 tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat
    23041 agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt
    23101 catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca
    23161 ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg
    23221 cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg
    23281 caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac
    23341 cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc
    23401 tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt
    23461 ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat
    23521 cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga
    23581 tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa
    23641 aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg
    23701 tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt
    23761 tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta
    23821 cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca
    23881 tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa
    23941 tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt
    24001 ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct
    24061 tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta
    24121 gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt
    24181 ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta
    24241 cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga
    24301 ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat
    24361 tgaagacact aacaagataa tcatttactg tttttaaatc tgattccatg ttccaccgtc
    24421 cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa
    24481 aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat tttactcatt
    24541 taaaacaact gataaatcag catttataat ttcttttatt agaaaaatca tctatctcac
    24601 atattttgat tatcaaagta ttttctgtga acagaactat agaattccct aaaacttgtc
    24661 cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga tcctatgtta
    24721 aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa agaccgagat
    24781 ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag cagctatttt
    24841 catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat aacagatttt
    24901 gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca attgatccaa
    24961 cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa ttatgtacat
    25021 attaccaata agggatttgt attaacagat actcctacgg ctcgaacata cgggcctttt
    25081 cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta tcaaatgcct
    25141 tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct acaggaaatg
    25201 acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa taaaaagcta
    25261 ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt cgaaaaagcc
    25321 ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat ccaggaacaa
    25381 aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc agatgagagt
    25441 cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat tacctctggt
    25501 gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa
    25561 ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa aaaattagaa
    25621 aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca actaaattag
    25681 ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc taaaatttat
    25741 tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc gtattgctga
    25801 tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt tactgtacac
    25861 ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc tgactcgacc
    25921 atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg aggtcctcct
    25981 tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat tttctctaaa
    26041 aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc aaagtttgat
    26101 caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat atagtttcaa
    26161 tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta atgcaatctt
    26221 taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg
    26281 aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg tctcaatcac
    26341 tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca ttcctgtcat
    26401 cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc cagcagaacc
    26461 atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat cataaacggc
    26521 ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt caccaccgga
    26581 taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc
    26641 caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa
    26701 aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta ccattgtaaa
    26761 gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag ctccttctgt
    26821 tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt gtcctgcttc
    26881 tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac caagtttcat
    26941 tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg ctagaaacaa
    27001 ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat tttattttga
    27061 ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga taaacaacca
    27121 tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata tcataaagtt
    27181 tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca ataagtgctg
    27241 gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg aacaatcggt
    27301 tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct tctgtataag
    27361 taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct ttataataaa
    27421 gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct aactgcagtt
    27481 ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata actaatctgg
    27541 cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg atatttttct
    27601 cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga cctagtgact
    27661 cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt tcaatgtcga
    27721 taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat gtatcaataa
    27781 cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta gacaagtatt
    27841 ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag tcttcataca
    27901 tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc ttcccaagta
    27961 tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct ttggcaacca
    28021 aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta
    28081 cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg gtcgttgtaa
    28141 aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg atcgatgtag
    28201 caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc atgtttttcg
    28261 cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac tttttcattc
    28321 agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact tcgctctttt
    28381 gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat ctgttggatc
    28441 agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc ttctaaccag
    28501 ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa
    28561 acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc acctgaatac
    28621 gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc aggatgatct
    28681 tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg accaaaccaa
    28741 ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat tcctttacgt
    28801 tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt gaaatattct
    28861 ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat cccattggaa
    28921 atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga ataaggtgtt
    28981 ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt ttcgatagga
    29041 gaaagctggt tcgatccgat aaaggaattt cggaaatctt cctctgtaga atgcgcatga
    29101 tagacaactt tttttccttc tcttctggct ttattgacca tattatgact attgattccg
    29161 tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata agagataccg
    29221 acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga ttttgcaagc
    29281 atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc acttatatag
    29341 tataacatag cctacaaact gtcactcaaa cttaagccct atttttgtct attcttactg
    29401 acaaaaacgt tttcttaaaa aagaacaaaa aaactggcgt agctctttta caagccacac
    29461 cagcttttga tacgttgttt attttacata ttcatgaaca agttcaacga cttcttccat
    29521 catatcgcat tctttcaatg cacgatcagc aagttcagcc attttcgctg tgtccaagcg
    29581 tttcattaaa ctacgcgttt taagaataga tgtcgcactc attgagaact catctagacc
    29641 catacctact agcaatggaa cagctgtttg atctccagcc atttctccac acatacctgc
    29701 ccattttcct tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta aaattgatgg
    29761 gttatatggt tggtacaagt aagaaacgcg ttcgttcata cggtctgctg ccattgtgta
    29821 ttgaatcaaa tcgttcgtac ctacagagaa gaaatctact tcttttgcaa acttgtctgc
    29881 taagactgct gctgctggaa tctcgatcat gatgccgact tggatcgtat cagatacttc
    29941 cttgccttca cttatcaatt tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa
    30001 ttctttgagt gtagctacca tagggaacat gatacgtaaa ttaccatgaa cagatgcacg
    30061 aagcaatgca cgcatttgtg tacggaacat ttcgtcgcct tgttcagata aactgatacg
    30121 caatgcacga tatcctaaga atggattcat ttcatgaggt aattgcagat atggtagttc
    30181 tttatctcca ccgatgtcca ttgtacgaac aactacaggt tttccttcca taccttctaa
    30241 tacagctttg taagcttcat attggtcatc ttctgttggg aagtctggag aatccatata
    30301 caggaattca gtacggtaca gaccaactgc ttctccacca ttattatgga caccaactag
    30361 atcttttggt gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt
    30421 ttctgcattt tttaattttt cccattctgc tttttgtgct gcgtaatctg cacctgcttt
    30481 ttcaaattca gctttttgtt catctgtagg atcgatgatt acatcgcctt cgatcccgtt
    30541 gacagccaaa atatcgcctt ctttgacttt agcagtaatt tcttttgtcc caacgattgc
    30601 tggaatttct aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac caatgtctgt
    30661 tacaaacgct tttacaaaat tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg
    30721 tgcaacaacg accacttctt cgttgatcat tgatggattt ggtaaagtaa cgcctaataa
    30781 gtgcgctaaa atacgtttag caacgtcacg aatatctgca gcacgctctt gcatataagc
    30841 attatcttcc attgcttcga acataccgat atacatatcc gttacttctt ttagcgctga
    30901 ttcagcattt acgctgttgt ctttaatatt ttgcttgatt tgacctacca tttctggatc
    30961 tgaaagaacc atcaaatggg catcaaatac ttgcgcttct gcttcaccta agctttgcgc
    31021 tgctttttca cgaatttgct gcaattcttc agtagattta gctaatgcag catccaaacg
    31081 agcttcttca gccgcagtat cttctactgt tgccttactg aatgttaaat ccggttgaac
    31141 tagcaggtaa gcttttgcaa cggctacacc gtcactagcg gcaatccctt ttagcatttc
    31201 aaccattatt cagataagcc ttctttcttc attgtttcaa cgattgctgc cattgcatca
    31261 gcttcgtcag caccttctgc agtaatagta acatcagaac cttgacctac acctaaagac
    31321 ataacgccca tgattgattt caagtttact gatttacctt tatactctaa gtttacatca
    31381 gagttaaatt tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg gatcccagtt
    31441 tctgctacta cgtgaaattc tttcttttcc atattaatcg gtctccttta agtgagtgtt
    31501 gtttttatgt gagggttttc ttttttagta attcagagaa tcctaacaaa tgccaccctt
    31561 tcatctgtaa agattaacat tttttttcgt tagatacaac agttttcatt caatttcaga
    31621 taaaagtatt caaattattc cggattcttt ttcatccttt tcttcacatt ttataacaaa
    31681 cgtgtttatt tcctttttct tgtcgctttc tcttctcctg acgtttatca ttctttcctt
    31741 tgaccctctt tgttttcata tgaaacgaat tacttgcttc cctttttatt tttagtataa
    31801 taagagtcaa aggtcaggaa tggtcaatag agatttcacc ttttctacct cataaatctc
    31861 ttaaaaaact aggggttatt cttttacctc atgaaaaacg agatgtaaaa tatggatgta
    31921 cccatttttg tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg caagaatgag
    31981 gcaacgattc acttgtatgc cactgtcaat ggtcagcgga cacaactcga ttactgtcag
    32041 agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat ggcccaaaat
    32101 gatccatttg gatttggcag tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa
    32161 cagcaaggaa attatgagca aacaccacca acacaatttg gcggaaacaa caacttcaat
    32221 ggtggtcagc cacctcaagg caatacagaa ggtttattgg gagaatacgg aatcaatatc
    32281 acagaacaag cacgacaagg tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa
    32341 cgagtcattg aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat tggagaacca
    32401 ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta
    32461 ccgcaaaaat taatggacaa agaagtcatt cgtttagatg tcgtatcttt agtccaaggt
    32521 actggtatac gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa
    32581 gcagaaaatg tgatcctatt catagatgaa gttcacgaaa tcgtcggtgc tggttctgct
    32641 ggtgatggaa atatggatgc tggtaatatt ttaaaaccag ctttagctcg aggagaattg
    32701 caaatggttg gtgctactac cttaaatgag taccgcatca tcgaaaaaga cgctgcttta
    32761 gaacgtcgta tgcaacctgt acgggtagat gaaccaactg tcgaagaaac gatcagtatt
    32821 ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg taaaatatac tgatgaggcc
    32881 atcgaagcag ccgcaacttt atccaatcgt tacgtccaag atcgcttctt gccggataaa
    32941 gccatcgatt tgctagatga aacaggttct aagaaaaact tgacgatcca aatcgttgat
    33001 cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt agcttctcga
    33061 gaagaagatt tcgaaaaagc agcttattat cgcgatcaga tcaataaact tcaaaaaatg
    33121 aaagaacgtc agctcactga agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa
    33181 atcgttgaac aacggacagg tattccagtg ggtgaattaa aagaaaaaga acagacacaa
    33241 ctgaaaaact tagcagatga cttgaaagct catgtcatcg gacaagataa cgctgtagat
    33301 cgagtagcga aagcgattcg ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc
    33361 ggctccttct tatttgttgg accaactggt gtaggtaaaa cagagcttgc gaaacaactt
    33421 gcctacgaac tattcggttc acaagattca atgattcgtt tcgatatgtc tgaatatatg
    33481 gaaaaacata gtgtctctaa attgattggt tctcctccag gttatgtcgg atacgaagaa
    33541 gctggtcaat taacagaaaa agtacggaga aatccttaca gtcttgtctt gttagatgaa
    33601 gtagaaaaag ctcatccaga tgttttgcat atgttcttgc aaatcttaga tgatggtcga
    33661 ttgacagatg cacaaggaag aacggtcagc tttaaagata cgatcatcat tatgacaagt
    33721 aatgccggaa ctggtaaagt agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt
    33781 actcgttctg tattgaacca attaaacaac tactttacac cagaatttct gaaccgtttt
    33841 gacgggatca tcgaattcag tgcattatca aaagaaaact tgatgacaat cgtcacacta
    33901 atgctagacg atgtcaacca aatgctggca gcacagcaac ttcacatcga agttcctacg
    33961 aatgtcaaag aaaaattagt tgacttaggc tatgatccat ctatgggtgc tcgtcctctt
    34021 cgacgcacga tccaagaaca aatcgaagat ggtatcgctg aattttatct tgatcaccct
    34081 tctatccatg agttgaaagc aaaactggat aaagatggaa aaatcatcgt tacttctaaa
    34141 ccagaacgtt tggctaaaga atcagccgaa gaaacggctg aataaatcca gttttatagc
    34201 gtgagacggt atttctttga gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac
    34261 tgttcatttt ataaaaaaga aattataata agagaaacaa gcaattaaaa atacataaaa
    34321 gaggtgagcg ccatgaaatt ggtaaatgta acaaacagcc attcccgtct tgtattaaac
    34381 cagcttgaaa acactgatgc ccatctagtc aaggtctaca cagccggaaa taccactatt
    34441 gtctacactg aagcccctga acataatgaa attctgctga tcaatgacaa acgtaaaatc
    34501 cagccgaaag aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa agacgccgtt
    34561 taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca
    34621 aaacatgaat taggcgctac attagccgtg taaacaaatt ttgaacataa taattaaaga
    34681 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgaa
    34741 agcagaagca actccttcgg aaataagctg aaattcacga aaattgcttt tcaaattttt
    34801 gtgaattcca gcttatttct tggagttaaa cgcttctgtc tcagcctctt tttctttcta
    34861 gctttttgat ttttctcttt tcttttgtac ttttctcttt tcattttcag ttctacgcgt
    34921 gtctttattt ttaacttttt cctctttatg gatgattgat cgtacttttt gtgcaatttg
    34981 ctcgtttttg tcccagtctt ttacatctaa taatcgacat aatcgtaaca tctcttcttt
    35041 tttctgtttt tttctttcac gtctttgcca ttctgtttcc atgtgtcaca ctctcctttc
    35101 tttaatcata tgtacgcaaa ataattttta tatttttttg attagacatt tctatgattt
    35161 taatatagta aaaagagccg tcacggaaaa tccgtcacgg ctctaaacaa aatatgagag
    35221 gctgtttttg tttggctatt cttattactt gatgcagggc agcttgtgtc aagacttctt
    35281 tacatcaaac tttttaattt tacattggga tatttatccg caaaccagcg ttctgcaaat
    35341 tggttctcga ataagaacaa cggttggtcg aaacggtcac gagccaagat attgcgactt
    35401 gaactcatct tctcatccaa atcttctgga tcgatccagc gagcgatttt gtgtcccatt
    35461 ggagacatga ttacttcggc gttatactca tttaacatac gatattggaa tacttcgaat
    35521 tgtagctgac caacagctcc aatgatatat tcttccgtta gatatgtttt ataaagctga
    35581 atcgctcctt cttgaactaa ttgatagatt cctttgtgga aggatttttg tttcatgaca
    35641 ttttttgcag tcactttcat aaacagctct ggagtaaatg aagggagttc ttcataagca
    35701 acttttagtt ttccttcgaa caatgtatcc ccaatctgat aattgcctgt atcatataca
    35761 ccgatgatat cgcctgctac tgctttttcg acgttttctc tcgaatctgc cataaattgt
    35821 gtgacattgc ttaatttcag ttttttattt gtacgttcca accagacatc cattcctcgt
    35881 tcgaatgttc cagaacagat tcggacaaaa gcgattcggt cacgatgggc tggattcata
    35941 tttgcttgga tcttgaatac gaaacctgaa aattcttcct cataaggact taccatctga
    36001 tcatcttgtg ttttgtgtcc ataaggagct ggtgccaatt caacgaaagt ctctaaaaaa
    36061 gtctgtacac cgaagttagt caatgcagaa ccaaaaaata ctggtgtctg atcgccacga
    36121 gcaatttttt cttgattaaa tgcatctcca gcctctttga ttagttctac ttcttctaag
    36181 acttgttcat ataaggaatt ttcagttaga ggatgatttt cgggaagcag tccatcttca
    36241 tccaattcta ctaatcgttc atcctgataa ttttccggac gataaaactc tacacgatta
    36301 tggtagatat cataaagacc ttctagccct ttacccatac cgattggcca attcatcgga
    36361 taagattcga tatctaatag ctcttctaat tcttctaaca attccaatgg ttcacgacca
    36421 tcacgatcta gtttgttgat aaaagtaaat atcgggatgc cacgtttttt tacaacttgg
    36481 aataatttct ttgtctgtgc ttcgatccct tttgcactat cgattaccat gaccgcacta
    36541 tccacggcca tcaaagtacg ataagtatct tcagaaaaat cttcatgtcc tggtgtatcc
    36601 aagatattta ctcgtttccc ttgataatca aactgcataa cagaactagt aacagaaatc
    36661 ccacgttgtt tctcgatttc catccagtct gatttggcaa aattacctgt ttttttgcct
    36721 tttacggtcc cggcttgacg gatagcccca ccaaacaata gtaattgttc tgtaatcgtt
    36781 gttttcccag catccggatg ggaaatgatc gcaaaagttc tgcgattgtc cacttgttgc
    36841 tttaattttg gattattcat aacctaactc catttcttcc attcaattat ttatattttc
    36901 tctaggcaca gattgtccga tctcttaaag aaagacaatt tcatataggg ctgtgacaaa
    36961 agccgtgtag cagtctcttt ttccgagtaa acggcgttct agagacagcc tatcagcaat
    37021 aagcccaccc aagccaaaaa tacgaaaagc tgtttttgct tggctgctct tattacttaa
    37081 tgcaggatag ctttttcaca acctttgtca actactacaa ctgaatcagt atagcatgga
    37141 ttcagcgaat gaaaaagcca ccaaaccata agagtcaaat atttttttgt cagctgacta
    37201 aaagaaaaga cgtgttgctt gtttattcaa gcaacacgtc tttcataacc aagtttccaa
    37261 ttatcttctt ggctcgtcat cttcgaattc ttttctgaag tatcttcgtt cttcttcagg
    37321 ttccgcttct gtttcttccg gatcatgaaa aatgacacgg attttaagga ttcgagaacc
    37381 ttccatttct tcagaaacca gcgtaatatt atcgacatca aacgataact tttcgccttc
    37441 atccggaatc atgcctaatg ctgtaatcaa ataaccagcc atcgtatcta catcactcat
    37501 atgcagatca ctgtcaaaag cttcattgaa ctcatcaatc agcatacgtc cttggatgat
    37561 atattcgtgt tcatcgattt tttcataaag attctcaact tcatctgatt catcatcgat
    37621 ttctccgacg atttcttcta aaaggtcttc tagtgttgct aacccaacga cgccgccata
    37681 ttcatctaat aaaatagcca tctgattctg cgtctttttc atttcatata acagatcatc
    37741 aataaagacc gtttcaggaa cgaataaagg ctcctgcatg attttcttga tatctaaatt
    37801 atcaaagccg aatttatggg cagcttttag taaattcttt gtatgaagga ttccgactac
    37861 tttatctttg tcttcgttat aaacagggat tcttgaatag ttttcggata aaacttcatt
    37921 cacattttct tccacagtgt cgttgatatc aatcataaat gcatccgttc gtgggaccat
    37981 gacttctcgg gcaactttcg tatcaagaga aaaaacccct tggagcattt ctaattcttc
    38041 attattcaat acgccttcgt tttcaagcat ataacgcatt tcatctctgg tcatttttga
    38101 atcttcatca tcaaaagtca tcggcgtgat acgcgccaat aaacttgtgg aagcagaaag
    38161 cagccagacg aagggcttag ctactacacc aatcaagcgt atcagccctg aagtgaactt
    38221 tgcgacttct tctgatttat ttaaagcaat acgtttaggg tacaattctc caaaaacaat
    38281 cgatacatag gtcaaaatag ccagcacaat gatatttgca atattttttg ccgctgtgcc
    38341 gccacctaat accggagcta accgttccga taaagtatta gctagtgaag caccagacaa
    38401 aatgttgacc aaagtgattc ctacttggat tgtcgatagg aaattatttg gattctgcaa
    38461 tatttttaaa agcttttgag atttcacatc tccttcatca gctttttgct cgattcgatt
    38521 cttattgatc gaaacaacag caatttcgga agctgctaaa aaagcattga tcaatgtcaa
    38581 aacgatcaat aataaaatct gcgctaacag cgactgactc tcagggtcag cattcataat
    38641 cgcattctct ccttataaaa ttaattttta gtttacgata gaaaaaaacg tggcactata
    38701 gccacgttaa aatggtatca caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa
    38761 ctacctacga ggtcgtagca attttcttct tgttttcatt ttgcttgtct tctttgacga
    38821 ttttgattat ataggaaatg atcgtcttgc agattgcgta aaaaggaaca cctaagaaga
    38881 taccaagcaa tccggcaata tttccagcta ctagaagaac gataatgatc gttaatggat
    38941 ggattttcag tgatttccca attacattag gataaatgat attcccatcc aactgttgga
    39001 cgattagcac aaccacacaa caaagtaatg cttttaccgg atcattgaag actgttacta
    39061 gaaatgcagg tgctagtcct agataaggtc ctaaatatgg aattaggttt gtcaatcccg
    39121 caatgacccc aaataagaaa gcataatcca caccaattac catatatcct aaaaatgtga
    39181 aggtaccaac gaataaacat tcgattgctt gtccgctaat gtaattagcc aatgttttat
    39241 tcagttgccc caataactcc acaatttggt ttcttctttt ttctgggaaa aactgttgga
    39301 tgtttggcac tagtttttct ccgtctttca acatatagaa caagatgaat ggtgctgtaa
    39361 tgattactac ggttgccgaa gcgatcgttc caacgatcga acctaaacta ttggatactc
    39421 cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc taattgctca aaatatttat
    39481 tgatatctac ttccttgaac aaaggcaatt cggctacctc ttttaaccag ctttctacct
    39541 gtttgatgaa atcaggcata ttagatgcca atgaggaaat ttgctgtact agacttggga
    39601 tgacactaag taaaatccaa ataattgctg caactaaaag gagcagcaca attgcgacag
    39661 cccatattct tttgattttt gctttcatca acagcttgac aataggattc aataaatagt
    39721 aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt
    39781 cgaaaataaa gtttatttct gatgaaatca tgatcaatgc agctatgatc aataattcaa
    39841 gcgaccaaaa cattagttta gactgtctaa gtttttcaaa caatagattt cctcctttct
    39901 ttcttactag tatatattat attcgttaaa aattctttta ttcttttttt
//
LOCUS       DACDEA010000022        39893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDEA010000022 DACDEA010000000
VERSION     DACDEA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.022"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..710)
                     /locus_tag="IV136_001133"
     CDS             complement(18..710)
                     /locus_tag="IV136_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8465139.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IV136_001134"
     CDS             705..971
                     /locus_tag="IV136_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8465140.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IV136_001135"
     CDS             complement(1147..1716)
                     /locus_tag="IV136_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8465141.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..2113)
                     /locus_tag="IV136_001136"
     CDS             complement(1790..2113)
                     /locus_tag="IV136_001136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465142.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2267..3079)
                     /locus_tag="IV136_001137"
     CDS             complement(2267..3079)
                     /locus_tag="IV136_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465143.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4281
                     /locus_tag="IV136_001138"
     CDS             3433..4281
                     /locus_tag="IV136_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8465144.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IV136_001139"
     CDS             4426..5367
                     /locus_tag="IV136_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8465145.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="IV136_001140"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="IV136_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IV136_001141"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IV136_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8465146.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9831..12017)
                     /locus_tag="IV136_001142"
     CDS             complement(9831..12017)
                     /locus_tag="IV136_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8465147.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IV136_001143"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IV136_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8465148.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="IV136_001144"
     CDS             complement(12239..12679)
                     /locus_tag="IV136_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8465149.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="IV136_001145"
     CDS             complement(12753..13292)
                     /locus_tag="IV136_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8465150.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="IV136_001146"
     CDS             complement(13907..15373)
                     /locus_tag="IV136_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8465151.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IV136_001147"
     CDS             complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IV136_001147"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8465152.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17907..18086)
                     /locus_tag="IV136_001148"
                     /pseudo
     CDS             complement(17907..18086)
                     /locus_tag="IV136_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18290..18649)
                     /locus_tag="IV136_001149"
     CDS             complement(18290..18649)
                     /locus_tag="IV136_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8465153.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18679..19020)
                     /locus_tag="IV136_001150"
     CDS             complement(18679..19020)
                     /locus_tag="IV136_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465154.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(19017..19691)
                     /locus_tag="IV136_001151"
     CDS             complement(19017..19691)
                     /locus_tag="IV136_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8465155.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IV136_001152"
     CDS             complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IV136_001152"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8465156.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22081..23271)
                     /locus_tag="IV136_001153"
     CDS             complement(22081..23271)
                     /locus_tag="IV136_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8465157.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23292..23819)
                     /locus_tag="IV136_001154"
     CDS             complement(23292..23819)
                     /locus_tag="IV136_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8465158.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(23866..26655)
                     /locus_tag="IV136_001155"
     CDS             complement(23866..26655)
                     /locus_tag="IV136_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8465159.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26802..27002)
                     /locus_tag="IV136_001156"
     CDS             complement(26802..27002)
                     /locus_tag="IV136_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8465160.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27454..27774)
                     /locus_tag="IV136_001157"
     CDS             complement(27454..27774)
                     /locus_tag="IV136_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465161.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(28277..29395)
                     /locus_tag="IV136_001158"
     CDS             complement(28277..29395)
                     /locus_tag="IV136_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465162.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            29868..31175
                     /locus_tag="IV136_001159"
     CDS             29868..31175
                     /locus_tag="IV136_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8465163.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            31293..32492
                     /locus_tag="IV136_001160"
     CDS             31293..32492
                     /locus_tag="IV136_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465164.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(32519..33787)
                     /locus_tag="IV136_001161"
     CDS             complement(32519..33787)
                     /locus_tag="IV136_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8465165.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33950..34597)
                     /locus_tag="IV136_001162"
     CDS             complement(33950..34597)
                     /locus_tag="IV136_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8465166.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(35005..35508)
                     /locus_tag="IV136_001163"
     CDS             complement(35005..35508)
                     /locus_tag="IV136_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8465167.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(35631..36395)
                     /locus_tag="IV136_001164"
     CDS             complement(35631..36395)
                     /locus_tag="IV136_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8465168.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            36502..36777
                     /locus_tag="IV136_001165"
     CDS             36502..36777
                     /locus_tag="IV136_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8465169.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            37373..38323
                     /gene="yidC"
                     /locus_tag="IV136_001166"
     CDS             37373..38323
                     /gene="yidC"
                     /locus_tag="IV136_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8465170.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(38502..39476)
                     /gene="lacD"
                     /locus_tag="IV136_001167"
     CDS             complement(38502..39476)
                     /gene="lacD"
                     /locus_tag="IV136_001167"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8465171.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(39473..39646)
                     /locus_tag="IV136_001168"
                     /pseudo
     CDS             complement(39473..39646)
                     /locus_tag="IV136_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
BASE COUNT        11419 a         8385 c         7059 g        13030 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctctcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat tcttggtaaa
     2281 gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag tgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgagaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacttct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgaa aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcacacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaagaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagtt ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aatcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccaaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcatat aataagaaaa atggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatctgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttaggtt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggaacgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttgacttcta gtttgatttg atcagccacg
    10381 gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtaacttcc
    11101 atgtgaagta gaccattcgt attgattgtc ccgccaaaga gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accatagccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattatctta
    12721 tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa caattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttagctcaaa
    13441 aagcggcaaa atatgaggag ctatttttcc gtttgtttct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaagtca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttccaaa gcttgcatct gcggtaataa
    13681 gcccagccaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt cttcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagacg attggtgtga tgatattcgt aaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaaaatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcact agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 gaccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttt
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttca
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgagt cgcccgtcaa catgactgtg ttcttcacac cagcctgttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttaaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ctggatatta gttgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aatatattgc
    16501 atgaatttct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcggtttgcg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gcgcctccga taccgccaaa gaaactaagt
    16681 ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccgcttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat cattgcccct
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaatccca tataaataaa
    18181 agacgtaaaa gtcacatggg agaacttata caggaaaaca catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgcgttttct aacctttatt attctctttt tatacaattt
    18301 ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta
    18361 tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta
    18421 ggcctgaaac gatcagccag atgatattaa cgatcaatga catgccagac tggtgataaa
    18481 ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga
    18541 ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac
    18601 cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt gtatttctcc
    18661 ttctattact gtttttgatt aaaactgggc aaaaattgat tttattcttc tttttttagc
    18721 ttccagcatc aagtattctg caaatttttt ttggatttct tccgtatttt cttttgttac
    18781 taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc acccgttctt ctgactgttg
    18841 cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag
    18901 tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc
    18961 agtaatcagc tgattgattt gaggtaaatc ttcttttact gcccaccgta tttccatcat
    19021 ttctcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc ggcaattgca
    19081 tctatccagc tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac cccatcttct
    19141 cttgtatatt tcgaaaaacc ataagcaatc cctaaagcat tgaatatgcc atctaaagga
    19201 accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag
    19261 tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg
    19321 atgttgcaga tttccgctgt ttgaagttca tttagtgatt ttcgataaac ggattcaaat
    19381 tgctgcgcag atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca
    19441 ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag
    19501 cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaattcc
    19561 atcgttggat attgggagat tagttttccg gcaatcaaaa gtggataacc tttacctaga
    19621 tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tgtcaccgat aaataatata
    19681 cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt
    19741 atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acaccagcct
    19801 agtcccttca atcaattgaa tgaacgctca tcaagtgtct agttttttat actttttttc
    19861 ttaacgtgcc ggattcccct tgattccttc gttgtttggt ccagagaaat cgatcacacc
    19921 taagcttccg tcaatcagac cactgatgac gccatcagaa catatattta taggctcatc
    19981 taagacttcc agtaaatgtt cttttactaa tatctcttct agattgcttg ggatagattg
    20041 gtcataactt tatctggtcc gatgatttca aggatgaatt aaacttacat atggatcatt
    20101 gcatgctgaa aatacggacc tactccatct tcgaatgtta ctattagttt gttttcaggt
    20161 actagatgct gctttatttc aacagcagaa gcatcatcaa atcagatttc catttgaata
    20221 accttctttg tatatgtttg attgattgcg atttcgttat agaaccttgt gaatcaaata
    20281 gcaatttctt ttgctttctt ctcttgtctg ctggaaactt cctttatccc gtattttaaa
    20341 aaaagaactg cgagagttcg cagttctttt ttttaaataa acgttgctct ccggccaact
    20401 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    20461 tgtgactcgg agctgaacgg caggcgcaca acctcttgat ataaacggtg ttcaatcagc
    20521 tgaccctcga tattagctaa aaaaacggta aaatatgaga agctattttt ccgttttgtt
    20581 tctctaatac tcaggtcaag gcactgattt cacaacctct tccttaaacg gtgttcaaaa
    20641 gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat ttccacaaaa
    20701 tttttcttaa tactcgaaac taaacagctt tttcatcacc tcttatggat aaattcggtt
    20761 caacaaacgt gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac
    20821 tgttcgttca aggcctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc
    20881 aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa
    20941 atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat
    21001 catatctgca caaattacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt
    21061 gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt
    21121 ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag
    21181 taattcgatt gcatcatcgt aagtaatacg agggaatggc aattttgtat aagtttttaa
    21241 gacttctttg tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg
    21301 tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc
    21361 catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc
    21421 acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta
    21481 taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc
    21541 cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcgattttga caaaatgatg
    21601 ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg
    21661 ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaaatctg ttccgtgttc
    21721 ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg taattccgat
    21781 ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt
    21841 ttcttggtgt agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac
    21901 tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga
    21961 tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc
    22021 gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat ttcttcagga
    22081 ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt
    22141 agcgcgatca gtggcataac tcatgcggac attttctggt gccccaaagc cttcgccagt
    22201 cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata
    22261 cttacacatg cgtagcgttt cttttacatt aggaaaaaga tagaaagctc cttgcggctt
    22321 ttcaagagaa acacccggca gttctgccac aagcggatat aacgtattca ggcgttcttc
    22381 aaaggcgttc cgcatttctt ctactgtatc ttgctcacct ttcaatgctt caacagctgc
    22441 atattgactg actgctgtcg gattgctagt tgattgggaa gcaacagcga tcattccatt
    22501 gatgatcaca ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt
    22561 agacacgccg ttgatgatga tggtctgatt tttgattgct tctgaaatcg ttgcaattgg
    22621 agtaaactca ttgccattat aaactaaacg gccataaata tcatcagcaa ctatcaaaat
    22681 atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc
    22741 agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc
    22801 tagctgttca acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag
    22861 tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac
    22921 ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc
    22981 tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac
    23041 gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccatt
    23101 gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc
    23161 cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc
    23221 tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat
    23281 tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc atagccaatc
    23341 aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca
    23401 tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc
    23461 tgttttgctt cattttcagt cagtcccttt gtttgatcca atacttttac tttttcacca
    23521 gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag
    23581 tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc
    23641 tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa
    23701 aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga
    23761 ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt
    23821 ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc
    23881 atttttcttt aagaattcat ggatatcctg atagatttcc tccatagcta gctctttgat
    23941 cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag taactagtct
    24001 tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc gtcctaatgc
    24061 ttggcgtaac ttcaaagttg ctttaggtac ggattcctgg taaaaaggat tgatcccttc
    24121 tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa
    24181 acgtgtgact acaacgatct gcaaagcatc tccaggcaaa tctacacctt cccaaaaact
    24241 gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg
    24301 gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat
    24361 tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc
    24421 ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct
    24481 gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc
    24541 ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagcctct tagagagata
    24601 gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg
    24661 ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg
    24721 ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca
    24781 ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aggctatata aacttgctaa
    24841 atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg
    24901 tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga
    24961 ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc
    25021 gttgggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc
    25081 gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt
    25141 cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg
    25201 tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa
    25261 atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg
    25321 tgtttcttgt actaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt
    25381 ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat
    25441 acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc
    25501 caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata
    25561 ttgtttttgt tctggcggtt ccacaagtgt tcctttaaat cgttgcaggt cgatatagtg
    25621 gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg
    25681 aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg
    25741 tttttcaggt gtagccaaat aactcaatgg tagcagataa ccgattgttt tacccattcc
    25801 tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc
    25861 attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt
    25921 tgcttgtttt tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac
    25981 ttcttttttc tgtaaagcta gcccatcgac gatttctaac gagggatcaa gtggctctgg
    26041 tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg
    26101 tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc
    26161 gatcagcaaa agaagttgac ctgtcacttc ggcatcacta tctgcctgat gaggattctc
    26221 atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa
    26281 gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca
    26341 tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat
    26401 ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg
    26461 gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg
    26521 atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa
    26581 ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata
    26641 agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata
    26701 ctacaaagtg cagaaaaata ctctcatcgc tttttaaaga aaatagacaa aaacgcccag
    26761 acacgcaaat gtctgggcgt ttatttttta gtataggtta attaagcttt agtaacgttt
    26821 gttgcttgag gtccgcgttg gccttcttca acatcgaaag ttactgcttg gccttcttct
    26881 aaagttttga atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt
    26941 tctgcagtga tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc
    27001 ataataaaaa tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat
    27061 ctggaagatg tttatttgaa acaagctacc acagattacc gaacaaaaac tactcctcta
    27121 gtatatcata ccgtttacaa aaaagctaga gtttttcgac tttttttatt aaaaaaagtt
    27181 ttctcagcga ttacataaag cgaaaaaccg aacttttttt aaaataaacg acatattgtt
    27241 cagttggtgt ttttttgctc tgatgttctt ttttaaacta aaaaaattat tctttctgac
    27301 tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt
    27361 tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga
    27421 tcaacgagat tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg
    27481 tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagatatg
    27541 ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa
    27601 agaccgttct tctgtttcag gaatcgcaga atcaagatct gttgtctgta cataaacatt
    27661 tttgtacaga cgtgtgcctg taataggaga ccgctgtgtt tcttcagcaa aaactggctg
    27721 acttgttaaa cttagggata gaaacagcaa acaccataac agtctttttt tcataaagac
    27781 ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca
    27841 ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc
    27901 acaacctctt ttttaaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    27961 acaaaaattt gagaaataat ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc
    28021 tgtcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccacc
    28081 aaacaaaaaa tatgaagagc tatttttgct tgctgttctt attacttgat gcagaacagc
    28141 tttttcacaa cctctcatac acggtgttta atcagctgac cttcggtatt agctcaaaaa
    28201 acggaaaaat atgtgaaact atttttccgt ttgtttctct aatactcagg tcaaaacact
    28261 gatttcacaa cctcttttaa taacgaagaa tcgctgtcag actcttttca tctggcgcat
    28321 ctttttgatc caaaataaac actttcccgc cattaatgat cacatcatct gctaatgcat
    28381 tcaacacttg acgccgatca tattctgttt caggatcttc accgaaacca tcagctaagt
    28441 ttgacgtagc gataaataaa tgtgacactt ttccttcttt tgcagctgga acgatatcaa
    28501 ctaactgatc gatgaatttc ttatctaaga gtttattgta ttcttttgat tcttgagctg
    28561 cgagttcact tgacagcttc ttcgcccctt cttcgatatc tctaaaggat agctgcgcag
    28621 gagaagcaga aatcgcaacg tctgcacaat agtaaggatt tttggcgatt tttttgaaca
    28681 tcgtctgatt ttctggtaga gcaaataaat aaatcggcca ttgttctgga ttttcgaaag
    28741 aatccttcaa gtacgtatca actgcttggt aatagttgac ccaatcgatc tctacttctt
    28801 catcttttgg atttacgcca tggaaagtta ctccatctcg ggctccgcct cctccattag
    28861 aagagaagtt caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg
    28921 gtgccccttc tggtagagat acttctttta cttgtttgtt gctgacttca tacaatttca
    28981 tggagtcgcg gttcaatccc aataaataat aagaatagtt gaactgccca tttttgataa
    29041 tagccagtaa ataaggaaga tcacttacat agtattgatc gtctaccggg atactcaaac
    29101 gatggatata ggtttggtca gcagttataa tgaccgctac actcgtagtt gcactacgcc
    29161 aaaacgaagg atctcctaac aaattttcaa ttttttcttg gaacggggac cagtcatgtt
    29221 cggaatattt tttctcaaaa cgtttctttg cttctttcga aaaatttttg aatttgatct
    29281 gatctttttc cacatcttga tgggcgacat gcgtattcag catgatcgtg acgaatggtc
    29341 ctttagcgcc ttctgaaatc aattgtgaca ataattcttt atcctttttt gccataaacg
    29401 tacccctcct aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca acactatttt
    29461 tccatacaat aaaacgtatc taaaaaaata aaacggtttc tacggtttta aatttagcta
    29521 tttttttaag ttatcttcat aatttcctca attatttagc cccagtcttc aactattctt
    29581 catgaaatgc tgataaacta agaaaaagaa taaaagaggt gtatcttatg catttgatta
    29641 tgattttcag tttattaagt attatcagtt tatttgcttt cagcgcttcc cgcttattag
    29701 aggcggaaac acagcctgtt cctataaaag tacgaaaaaa tgaccgctaa ccttttcata
    29761 acgcgcgttc ttatcctaaa gtttttttca gaaacattga aactctcttc cttctctgat
    29821 aaaattttaa tttatagcga aacaaattaa atacaaggag atcggatatg tccataaaaa
    29881 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    29941 ctgttgctgt gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtgg
    30001 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    30061 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactat
    30121 catgggatga taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt
    30181 tatcgaatgt tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg
    30241 caatcatcca gtcagccaat gcttcaatgg tggccttggc agaaaaagtc tctggcagcg
    30301 aagctaaatt cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa
    30361 cgatcgttaa tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca
    30421 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt
    30481 tgatcttgga tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg
    30541 aaaatacgca atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta
    30601 attacaagga aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt
    30661 tcgttggtac gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg
    30721 gccatgcgga aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct
    30781 atgacaattg gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agcctaaaaa
    30841 ccattgatgt aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa
    30901 aagtatgggt acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca
    30961 agaagcaagt cactgacaac gagatccaag cacctatgga taaaggtaca aaaatcggta
    31021 ccgctacgat caccttggca gacgataaat taggttattt agaagataac aatgcgccat
    31081 cgacagagat cattacggat aaaacagtgg aaaaagcgaa tatgttcgtg attggctggc
    31141 gacgcgtcgc agaatttttc ggcaacttgt tttaattgaa caataagaaa agcatctttc
    31201 catcggaaag atgttttttt gctttttcta gtttaataac agcttttttc atctattttc
    31261 gttatactgt ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt
    31321 tagttctcta tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc
    31381 cttccttgtt cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg
    31441 tgttggaaat tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct
    31501 ttgctttttc ttatttcaca gaaaaacgaa ggctgctgaa tggtctttta ttcaatgttt
    31561 tcctcatcag tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatattttcc
    31621 ttggcggact gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt
    31681 atggattgat tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt
    31741 cattagctaa tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg
    31801 actttttctt gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc
    31861 tgatcttgat ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc
    31921 agttcaaccg gcctcgatac aatcaagact tcatcgttgt gttaggcgct gggttgatca
    31981 atggagaaac agtttctcct cttctagcca agcgaatcaa taaggcgatt gctttttatc
    32041 gggctcaaag ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag
    32101 cagacgaaaa agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc
    32161 ctgaaagaga cattcttgtc gaaaccaatt cgacaaccac tttggagaac atgctctatt
    32221 caaaggaaat catggatcag caaatgaaag ggccataccg cgtgatcttt agttccaaca
    32281 attaccacat ttttagagcc ggtctttatg cgtgtcaagc caaattgaaa gcaaatggta
    32341 ttggtgcaaa aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacatcgcca
    32401 tcttgatgat gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg
    32461 cattcttatc gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc
    32521 acaccttgtt tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga
    32581 ccgctacttg ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc
    32641 agatcataaa gaagtatact gcagcttgtg aaccgaacaa ttcgatcccc atcacaaagc
    32701 aggcaatcgg tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa
    32761 aaggaacaga aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa
    32821 acaatggtgt cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata
    32881 acttcccaat aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa
    32941 gactcaatcc gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc
    33001 cacctatcgt atttcgccaa acaggatgag gaatccattg tgcatatcga gcttttaaaa
    33061 agacgatcga acggctgaat acccagccaa tcaaaccaaa agcaatccca gcaacaaaaa
    33121 ctttgaagaa cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag
    33181 aaacaccata gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga
    33241 tcgcttctgt tcggactttt ccgattgcca acacttctaa tccaaataat gttccagcta
    33301 aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc
    33361 gttctgattt atccaatcgg aacaaacgtg ctgtattatc tgctaacgct ccccccattt
    33421 ggactgctgt tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa
    33481 acagtgtaag tggaataagt cgcaacggga ttttttcttc tccaccattt ccttgatcga
    33541 tcactaagtt gtttccacga ctagagagac cgccgtgata cacatagaga taagcaaaaa
    33601 gcgcaccact aactggtagt agataaagga gccaaggatg tgatagtcgg gtatctgtca
    33661 caactgtcaa gctatttaag aaaaaagcag ataagctccc catgaatata ccgagaatcg
    33721 ctgtgatcac cagccacttc agcatataca ctaaaatgcg cattggactc ggtaattgtt
    33781 tttccataaa aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca
    33841 gactttagta ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct
    33901 attcacttcc attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt
    33961 tctttacacg ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt
    34021 ggtcactatc atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt
    34081 tcgttgtaga aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct
    34141 tatttatcgt tggtgataaa gtaccactaa tcccttttaa tttttcatct tcgtaataga
    34201 ccatgaattt tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt
    34261 cgatttcatt ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt
    34321 gtggtttgct aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt
    34381 ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgatttcta
    34441 gcagttcgtg atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta
    34501 ccgtttttgg atcattgaca gatgagtacg catttaataa ctgttctact tctcttttta
    34561 taaaaaaata ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa
    34621 ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat
    34681 cccggactct tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat
    34741 tcttctatgt acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc
    34801 aactgcaaac agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg
    34861 aatggtgttc aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa
    34921 gtaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat
    34981 atggtctatt attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca
    35041 tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc
    35101 cacgaatgtc acgatatatc cttctttgta cttaggcggt gcgatcgttg caccccacat
    35161 gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt
    35221 tacagcaatt cttggatgga tatacgcatg tgttccctcg tcaaatttcg tcgtacgcca
    35281 atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc
    35341 ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt
    35401 ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc
    35461 ttgtacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgatct
    35521 gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg
    35581 attattctag tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa
    35641 aaatgataaa gaacgatccc tgctgcaact ccgacattca atgattccgc atttcctttc
    35701 attgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac tccctgacct
    35761 tcgtttccca tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct
    35821 tcttcattta attctgtacc gtacaccgta aaccctttgc ctttcattac agggattaca
    35881 tctgccaaat ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc
    35941 acctttgtac tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac
    36001 gcatctgccg tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa
    36061 agccagcctc ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac tgctaagatt
    36121 ccttgcggtg taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta
    36181 gaaatctctt tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca
    36241 aaaagttctt ttatttctgc atcagacgac gcagcttctt cgatcagatg gaacccttcc
    36301 aaaagatatt ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg
    36361 attcttggat tttttgctga ttggatctct ttcacattcc tcacctcaac tgattatatc
    36421 atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa
    36481 caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag
    36541 ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg
    36601 gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc
    36661 caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg
    36721 gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt
    36781 tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat agcttctcat
    36841 attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt caataaggtt
    36901 gcgaaaaagc tgatctgcat caagcattaa ggactggttg cgaaaaagtc gcttagtttt
    36961 gagtattaag gaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgtcttaa
    37021 taccaagaaa ctgacttttg aacaccgtcg ataaggttgt ttttgaggcg ctttgcctca
    37081 agtattaaag acaggtagta aaaaagctga tctatcactt gcagtgagtt catttgactc
    37141 ctctatttcg ggcagaataa aaaagttttt agttttttaa ataagaaaga aacacttgga
    37201 atcactcttt tctttccaag tatttctttc ttgatttttt gtcttttttc aaaatttttc
    37261 gctacgaaaa agaaagaata tttatatgaa atttctatga agttgattga attccatgcg
    37321 cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa
    37381 atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg
    37441 tgtgcggatg gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt
    37501 ccatcctatg ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg
    37561 ggcagttatt gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca
    37621 atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc
    37681 agcacaacaa aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga
    37741 aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc ggcggaatcg gctgtctgcc
    37801 tctattgatc caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg
    37861 tatccgtgaa tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgttgc
    37921 cattgcaggg gtcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga
    37981 acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc
    38041 gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct
    38101 gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag tggcagaaga
    38161 gttgaaacaa aacccgccaa aacaagtcgt cactccaaga aaagacgtga cagaatcagc
    38221 aaagccagct gacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa acagtacaaa
    38281 caacaaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa ataagagtgt
    38341 aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt ttgaaaaaga
    38401 aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat tgatcgctat
    38461 ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc aacgtagaca
    38521 ggtgttgctg tttcttttaa taatgtactt aattccttaa tattttgttt tccttcttct
    38581 aacatccaac tactcgcttt tttttcgcct tcggtaacaa aaggtgccac tcccttcgcc
    38641 catgttgctc gcccacataa gataccattg aatgtcgaac ctgattgttt tgcaaaacgt
    38701 aatgtatctt gaaatagcgt agcattgact cccgcactta aaaaaataaa cggttgttca
    38761 gtcactttcg attgttcata gaaatacgcg gatgcctctg cttgactata acaagtctct
    38821 tgcccatatc cttccacaaa tctcatattc actggcactt ctactttcaa tacatccact
    38881 tgataacgtt cttttgaaaa ttctcgcatg gcttcaataa ctttatgggg ttttactttt
    38941 gcataggcaa gagaagacgg atcgccaata gttgtatcat aagaaaggat ctctaaaaag
    39001 aaaggtatgc cttctgcttc gcattctgaa ccaatccgtt ctataactat ttgtttttgc
    39061 acattaatct ctgctggttc atccacgtca tagtacaata aaaatttaca agcatccgca
    39121 ccagcttctt ttaacctttt caccgaccaa atattcaata aatcaggtaa tcttcccggc
    39181 attttcgtat catagccggt tttttcataa gctagcagca agccacattc tttctctcga
    39241 agcgctgctg caggcaggcc gtattctgga tcaagtaaaa tagctgaggc atagggagtc
    39301 aggtattgag aaaccatctg tttaaattgg atgatgtctt ctgatgtagc ttctttatat
    39361 tggttgatca ttttcttcaa agcaccacgc tggtcgattg ctaacgcgct aataattcca
    39421 gcttcatccg tcaatcgctt caaacgttcc tgcttttcta tactcatctt cttcatatat
    39481 cttcacctat ttctttagtt aatattgtgg ctatttttgt ttttgatttt gcttttctca
    39541 actgttctgt aacctctttt cgcatcaatg atcgtgcaac gagagacaat aatttcaaat
    39601 gagtcgttcc tgcttctgaa tctggaataa ataacgcaaa aataaaatag taaatatcta
    39661 ctcgtgaaaa cgggtggttt ttggcagccc tagaaggcct catcacttgc ttatcctctt
    39721 aagaggggct ttttaaggga ccaccaactt gtaccgaccc ccaaaagtta gaccaaaaaa
    39781 tctaactttt ggaggtcggt attttttatg gctaagtata cttttgaact taaattaaag
    39841 atcgttcatg attatttgga tggcaaaggc ggaagtgatt atctggcaaa gaa
//
LOCUS       DACDEA010000023        39155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDEA010000023 DACDEA010000000
VERSION     DACDEA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.023"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..3447
                     /locus_tag="IV136_001169"
     CDS             169..3447
                     /locus_tag="IV136_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8465172.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3988..5895)
                     /locus_tag="IV136_001170"
     CDS             complement(3988..5895)
                     /locus_tag="IV136_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8465173.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6025..6900
                     /locus_tag="IV136_001171"
     CDS             6025..6900
                     /locus_tag="IV136_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8465174.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6980..7954
                     /locus_tag="IV136_001172"
     CDS             6980..7954
                     /locus_tag="IV136_001172"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8465175.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8143..9279
                     /locus_tag="IV136_001173"
     CDS             8143..9279
                     /locus_tag="IV136_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8465176.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9416..9760
                     /locus_tag="IV136_001174"
     CDS             9416..9760
                     /locus_tag="IV136_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8465177.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10031..11317
                     /gene="efmA"
                     /locus_tag="IV136_001175"
     CDS             10031..11317
                     /gene="efmA"
                     /locus_tag="IV136_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8465178.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11455..12054)
                     /locus_tag="IV136_001176"
     CDS             complement(11455..12054)
                     /locus_tag="IV136_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8465179.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12458..13579
                     /gene="mnmA"
                     /locus_tag="IV136_001177"
     CDS             12458..13579
                     /gene="mnmA"
                     /locus_tag="IV136_001177"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8465180.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13825..15246
                     /gene="arcD"
                     /locus_tag="IV136_001178"
     CDS             13825..15246
                     /gene="arcD"
                     /locus_tag="IV136_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8465181.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15260..16378
                     /locus_tag="IV136_001179"
     CDS             15260..16378
                     /locus_tag="IV136_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8465182.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16438..17730)
                     /locus_tag="IV136_001180"
     CDS             complement(16438..17730)
                     /locus_tag="IV136_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8465183.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17880..18440
                     /locus_tag="IV136_001181"
     CDS             17880..18440
                     /locus_tag="IV136_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8465184.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18569..18946
                     /locus_tag="IV136_001182"
     CDS             18569..18946
                     /locus_tag="IV136_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8465185.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18879..20951
                     /locus_tag="IV136_001183"
     CDS             18879..20951
                     /locus_tag="IV136_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8465186.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21162..22109)
                     /locus_tag="IV136_001184"
     CDS             complement(21162..22109)
                     /locus_tag="IV136_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8465187.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22355..22819
                     /locus_tag="IV136_001185"
     CDS             22355..22819
                     /locus_tag="IV136_001185"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8465188.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22881..23924)
                     /locus_tag="IV136_001186"
     CDS             complement(22881..23924)
                     /locus_tag="IV136_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8465189.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24301..25266)
                     /locus_tag="IV136_001187"
     CDS             complement(24301..25266)
                     /locus_tag="IV136_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8465190.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25500..26345
                     /locus_tag="IV136_001188"
     CDS             25500..26345
                     /locus_tag="IV136_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8465191.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26536..27723
                     /locus_tag="IV136_001189"
     CDS             26536..27723
                     /locus_tag="IV136_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8465192.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27729..28085
                     /locus_tag="IV136_001190"
     CDS             27729..28085
                     /locus_tag="IV136_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8465193.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28087..28425
                     /locus_tag="IV136_001191"
     CDS             28087..28425
                     /locus_tag="IV136_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8465194.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            28820..29854
                     /locus_tag="IV136_001192"
     CDS             28820..29854
                     /locus_tag="IV136_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8465195.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29847..30533
                     /locus_tag="IV136_001193"
     CDS             29847..30533
                     /locus_tag="IV136_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465196.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30557..31405
                     /locus_tag="IV136_001194"
     CDS             30557..31405
                     /locus_tag="IV136_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465197.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31602..32375
                     /gene="sufC"
                     /locus_tag="IV136_001195"
     CDS             31602..32375
                     /gene="sufC"
                     /locus_tag="IV136_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8465198.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32388..33674
                     /gene="sufD"
                     /locus_tag="IV136_001196"
     CDS             32388..33674
                     /gene="sufD"
                     /locus_tag="IV136_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8465199.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33671..34906
                     /locus_tag="IV136_001197"
     CDS             33671..34906
                     /locus_tag="IV136_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8465200.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34893..35363
                     /locus_tag="IV136_001198"
     CDS             34893..35363
                     /locus_tag="IV136_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8465201.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35368..36759
                     /gene="sufB"
                     /locus_tag="IV136_001199"
     CDS             35368..36759
                     /gene="sufB"
                     /locus_tag="IV136_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8465202.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36854..37996)
                     /locus_tag="IV136_001200"
     CDS             complement(36854..37996)
                     /locus_tag="IV136_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8465203.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38035..38769)
                     /locus_tag="IV136_001201"
     CDS             complement(38035..38769)
                     /locus_tag="IV136_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465204.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            39001..>39155
                     /locus_tag="IV136_001202"
     CDS             39001..>39155
                     /locus_tag="IV136_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8465205.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGV"
BASE COUNT        12694 a         7195 c         8247 g        11019 t
ORIGIN      
        1 aaacttattc tttttacgtt aataaatctc tttattatca acaacttaag ttatattttt
       61 tctttttata aaatacactt attataattt tgttttttat tgacagtaaa aataaattag
      121 gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa
      181 cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt
      241 tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg
      301 aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg
      361 gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct
      421 gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg
      481 tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa
      541 ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc
      601 aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa
      661 ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt
      721 tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga
      781 caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg
      841 gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca
      901 gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat
      961 aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg
     1021 gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca
     1081 ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa
     1141 tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc
     1201 gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg
     1261 attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg
     1321 tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg
     1381 cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa
     1441 ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca
     1501 gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac
     1561 cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga
     1621 agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca
     1681 atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt
     1741 aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt
     1801 cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt
     1861 ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga
     1921 aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc
     1981 agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt
     2041 acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg
     2101 acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt
     2161 tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca
     2221 gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc
     2281 aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa
     2341 aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca
     2401 agttttaata acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac
     2461 taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc
     2521 cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa
     2581 cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct
     2641 ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc
     2701 catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg
     2761 gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa
     2821 tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat
     2881 ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa
     2941 ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt
     3001 gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa
     3061 ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc
     3121 acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc
     3181 cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca
     3241 gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat
     3301 ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga
     3361 gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt
     3421 tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt
     3481 gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc
     3541 tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag
     3601 gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     3661 ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc
     3721 aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta
     3781 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa
     3841 actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa
     3901 ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt
     3961 gaagctgacc acttctggca caacctctta ccagattcgc actctttttt ctggcgctag
     4021 atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg
     4081 ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg
     4141 gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat
     4201 agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc
     4261 aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc
     4321 gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca
     4381 ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga
     4441 aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg attgttctag
     4501 actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg
     4561 ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt
     4621 ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa
     4681 agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc
     4741 ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc
     4801 atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac
     4861 catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc
     4921 aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc
     4981 ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga
     5041 tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa
     5101 atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac
     5161 tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt
     5221 gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc
     5281 ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat
     5341 tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa
     5401 gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg
     5461 aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc
     5521 atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt
     5581 attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg
     5641 agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg
     5701 tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat
     5761 caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc
     5821 agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa
     5881 tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa
     5941 aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt
     6001 gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta
     6061 gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag
     6121 cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt
     6181 tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat
     6241 caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct
     6301 agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag
     6361 gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg
     6421 gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat
     6481 caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat
     6541 tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt
     6601 ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt
     6661 gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa
     6721 cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca
     6781 gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg
     6841 ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa
     6901 gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag
     6961 aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag
     7021 tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg
     7081 taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat
     7141 tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat
     7201 ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt
     7261 attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac
     7321 tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga
     7381 tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat
     7441 gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt
     7501 ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac
     7561 attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga
     7621 tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc
     7681 acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca
     7741 tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg
     7801 tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac
     7861 atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc
     7921 gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt
     7981 gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct
     8041 atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta
     8101 tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg
     8161 gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg
     8221 caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa
     8281 ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc
     8341 tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt
     8401 caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc
     8461 atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg
     8521 aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg
     8581 atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta
     8641 gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc
     8701 catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca
     8761 aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc
     8821 ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg
     8881 gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa
     8941 ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat
     9001 ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat
     9061 gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt
     9121 acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg
     9181 atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag
     9241 tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat
     9301 gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa
     9361 aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc
     9421 atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc
     9481 aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt
     9541 gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt
     9601 atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt
     9661 caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga
     9721 tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca
     9781 actagttttg aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt
     9841 tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt
     9901 gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt
     9961 gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag
    10021 agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt
    10081 gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat
    10141 catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact
    10201 aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga
    10261 taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc
    10321 agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat
    10381 gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt
    10441 tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt
    10501 catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat
    10561 tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc
    10621 aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg
    10681 gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt
    10741 gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg
    10801 tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg
    10861 cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc
    10921 ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg
    10981 gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac
    11041 actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg
    11101 gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt
    11161 agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg
    11221 cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa
    11281 actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa
    11341 gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg
    11401 aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga
    11461 cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt
    11521 gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac
    11581 atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc
    11641 gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac
    11701 gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt
    11761 tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt
    11821 tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg
    11881 catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct
    11941 agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt
    12001 ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct
    12061 tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct
    12121 aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa
    12181 atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga
    12241 gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat
    12301 gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg
    12361 aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt
    12421 agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg
    12481 cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga
    12541 acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg
    12601 tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat
    12661 tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct
    12721 agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa
    12781 attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca
    12841 ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa
    12901 taataaagat caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat
    12961 gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct
    13021 tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa
    13081 acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga
    13141 agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg
    13201 gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac
    13261 aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga
    13321 tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc
    13381 gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg
    13441 tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt
    13501 ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa
    13561 agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga
    13621 aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat
    13681 tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc
    13741 tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca
    13801 attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga
    13861 ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat
    13921 ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt
    13981 attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt
    14041 ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg
    14101 ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc
    14161 atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca
    14221 agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc
    14281 atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta
    14341 tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat
    14401 ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg
    14461 gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag
    14521 aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat
    14581 atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca
    14641 caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc
    14701 aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca
    14761 gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat
    14821 aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta
    14881 ttgactttct tagttactga ttatgcctat caatttgcct attctttatg tacagcggca
    14941 attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga
    15001 atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt
    15061 ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc
    15121 tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt
    15181 gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag
    15241 atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa
    15301 atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga
    15361 cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga
    15421 accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct
    15481 ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct
    15541 tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta
    15601 tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa
    15661 ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac
    15721 aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac
    15781 tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt
    15841 atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg
    15901 tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac
    15961 agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg
    16021 cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt
    16081 ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa
    16141 tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc
    16201 catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt
    16261 agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa
    16321 agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac
    16381 aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta
    16441 ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc
    16501 agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac
    16561 aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc
    16621 gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg
    16681 tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca
    16741 agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa
    16801 tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact
    16861 cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg
    16921 tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg
    16981 aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt
    17041 gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga
    17101 aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa
    17161 gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga
    17221 agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa
    17281 ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc
    17341 attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac
    17401 actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag
    17461 catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt
    17521 caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata
    17581 attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc
    17641 agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct
    17701 aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc
    17761 tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct
    17821 ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa
    17881 tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag
    17941 agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc
    18001 cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac
    18061 ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag
    18121 cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg
    18181 agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa
    18241 cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc
    18301 atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat
    18361 actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact
    18421 ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta
    18481 aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc
    18541 tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa
    18601 tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga
    18661 gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta
    18721 tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg
    18781 ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa
    18841 tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg
    18901 aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa
    18961 attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga
    19021 aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga
    19081 tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca
    19141 agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag
    19201 aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc
    19261 ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac
    19321 ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc
    19381 tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag
    19441 gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt
    19501 tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc
    19561 ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga
    19621 tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc
    19681 cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg
    19741 caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc
    19801 cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc
    19861 aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc
    19921 aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg
    19981 aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg
    20041 tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg
    20101 ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga
    20161 aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc
    20221 ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct
    20281 tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg
    20341 agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta
    20401 aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa
    20461 gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga
    20521 atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat
    20581 atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga
    20641 aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa
    20701 gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca
    20761 tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc
    20821 ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa
    20881 aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa
    20941 tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtctgcttc aagaaataag
    21001 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa
    21061 gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt
    21121 cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat
    21181 tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct
    21241 aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg gattccacca
    21301 atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat
    21361 gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata
    21421 aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg
    21481 aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa
    21541 atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct
    21601 gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca
    21661 tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa
    21721 ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca
    21781 gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca
    21841 tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct
    21901 ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc
    21961 ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca
    22021 tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta
    22081 aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat
    22141 atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt
    22201 aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt
    22261 atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga
    22321 ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt
    22381 tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga
    22441 aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt
    22501 tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca
    22561 gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga
    22621 agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac
    22681 caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac
    22741 aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga
    22801 aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca
    22861 actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact
    22921 gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat
    22981 tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg
    23041 ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa
    23101 aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca
    23161 gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca
    23221 acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa
    23281 ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa
    23341 agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc
    23401 aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct
    23461 ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct
    23521 ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca
    23581 ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga
    23641 gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg
    23701 ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat
    23761 aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga
    23821 agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc
    23881 gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc
    23941 gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa
    24001 gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca
    24061 gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    24121 aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt aaacggtgtt
    24181 caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac agctgttttt
    24241 gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt gaatcgatta
    24301 ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa ttaagaaacg
    24361 tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt ccgcaacttt
    24421 taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa cagtagtaaa
    24481 tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg gataaaaacg
    24541 aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt gagataaagc
    24601 gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat aacctgttag
    24661 gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat gagccatatt
    24721 tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc agatcaatga
    24781 aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta cagtgatgac
    24841 cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg aaagatcagc
    24901 tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga taacagcgca
    24961 cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat aagtacaaat
    25021 aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat catgcaacgt
    25081 ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca taagaatcat
    25141 agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa acagatttcc
    25201 aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt tcaaaaagtt
    25261 cttcatatat aatattctcc tttttattta atagatttta gtaccaagat gaacgaatac
    25321 attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt gtcttcatct
    25381 aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga tggttttttg
    25441 tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga agtggtgcca
    25501 tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt tccagagcag
    25561 cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag ttggagcggg
    25621 aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca actaagcaag
    25681 ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa actcagcaaa
    25741 aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc cacacctttg
    25801 ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct aaagtgcatt
    25861 ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa catgaatccg
    25921 attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac tctttgatgg
    25981 aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta agagaagcaa
    26041 actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc caagataaga
    26101 agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga atcggtcgct
    26161 cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg aatttccatc
    26221 gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta gagattgccg
    26281 aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag aatctcagct
    26341 ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa gcagtaggct
    26401 tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc ccaaatgtct
    26461 atggaaagac tttccattat ctaactggac cgctataata atcagcagta tggtttttaa
    26521 gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg tattgattat
    26581 ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt atatgtagca
    26641 cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca agttgtctgg
    26701 tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa atggatctgg
    26761 aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt gctcaaattc
    26821 tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt cggtgcgttt
    26881 actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc actgatcgta
    26941 actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg gctgattggg
    27001 aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga tgactttgga
    27061 acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg aatctcttgg
    27121 cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac gatcttcttg
    27181 gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc ttatcagttt
    27241 gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag ttatcagccg
    27301 gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt caacgtaagc
    27361 aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg tgaaaacttc
    27421 ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta tcgtatgatt
    27481 cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg tttgatcatg
    27541 atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact tcctttaaca
    27601 gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa catgatcggt
    27661 atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc ttcaaggagg
    27721 taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa aaagccaaag
    27781 cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa gaagtaccga
    27841 aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt cgcctgttca
    27901 acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc gagtttacgg
    27961 cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga ccactgatct
    28021 tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa ggagaactga
    28081 tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt atttaatgga
    28141 aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga catcacgttt
    28201 gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat cgaagttgaa
    28261 gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc ttctgttaat
    28321 gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa tgcgtggatc
    28381 ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca gaaaatcact
    28441 tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc tatatgaaac
    28501 gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc ctgtttgctg
    28561 aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc gagcgtaagc
    28621 aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag ctaagtccaa
    28681 gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac acggaagttc
    28741 aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg aaaaatcata
    28801 agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa agacattcaa
    28861 tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg aaaaagggga
    28921 tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc gtctcctgaa
    28981 tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata ttacaaaatt
    29041 aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc agcatttcaa
    29101 cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat tagctggcgt
    29161 accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg ggttagaagg
    29221 acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg tcggtatcgc
    29281 acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta gcgcattgga
    29341 cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga aattgaattt
    29401 gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca acaaagtcgc
    29461 tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct tcagacaacc
    29521 gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag ctgattcgac
    29581 agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa ctttgacctt
    29641 tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct ataacatcga
    29701 tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg gttcattgac
    29761 tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca tcaaagaaca
    29821 aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca tatctagatt
    29881 ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta tatatgacat
    29941 taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc ctttactcta
    30001 ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg atcatcagta
    30061 atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca tttactaaag
    30121 caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt ttatccgcag
    30181 caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct ggtgtactgg
    30241 aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc ttgatacctg
    30301 aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg atcggattta
    30361 ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg caggaaggct
    30421 accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta ctaatcgtat
    30481 ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga taataggata
    30541 tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc ttttgctgac
    30601 tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa ataaagcaga
    30661 cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt tggaacatgt
    30721 gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg atgactatgt
    30781 attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc agcatattcc
    30841 ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg gtgctgtcca
    30901 catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat taaaagatgg
    30961 tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca caattctaca
    31021 agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa cattcgatga
    31081 tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat cacttttagc
    31141 ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact tcgcttacgg
    31201 cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact ctccttacgt
    31261 gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa aactgattga
    31321 agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg gctctgttaa
    31381 accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa taagtctgta
    31441 atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa ttgtttaata
    31501 ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc tcaattagtc
    31561 taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc ttagaaataa
    31621 aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac ctaacaatga
    31681 aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct accttatctg
    31741 ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg tttgacggga
    31801 aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt ttagctatgc
    31861 aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca gcgatcaatg
    31921 cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag ttagatgaaa
    31981 aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac gaaggttttt
    32041 ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa cctacatttg
    32101 caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc gttgctaaag
    32161 gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac tatcaacgat
    32221 tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga gtagtcatga
    32281 cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt atcagtaaag
    32341 aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg aaagaaaaaa
    32401 actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc gaacctgatt
    32461 ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca ttgccgcata
    32521 tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca caaaccatca
    32581 gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg cttgtccaac
    32641 aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa ggagtcattt
    32701 ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa tattatatga
    32761 aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct tttatgaaca
    32821 gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc gagtccttat
    32881 tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc gtggcagatg
    32941 aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa gtagcaaaat
    33001 catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc aaatattcag
    33061 ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg cacattttac
    33121 gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg atcgctgact
    33181 ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg gcaatcagct
    33241 cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct catacgatcg
    33301 ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt aatggtatcg
    33361 gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt gtcttgatgc
    33421 tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa aacgaagtaa
    33481 ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac tatctgatga
    33541 gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc ctcggttctg
    33601 tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta atcgaaggga
    33661 agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct tatttcaaga
    33721 cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa agccaaaaca
    33781 agtactccag gcattagcgt actattacga acacgataac gccaatgttc atcgcggtgt
    33841 ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa aagtacgggc
    33901 gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga cgacaagctt
    33961 gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg agatagtgct
    34021 ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa aacgaacagg
    34081 tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg aggatgcagc
    34141 gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca atgtcttagg
    34201 tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg gggtcatcgt
    34261 agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac tagactgtga
    34321 tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg ttttatatgg
    34381 taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa tgatcgattt
    34441 tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag cagggacacc
    34501 aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa aactcggtat
    34561 ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa aattacaagc
    34621 aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag gagtcgttgc
    34681 attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca tggaaggggt
    34741 agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg aagtatcagc
    34801 gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc gtttagttga
    34861 agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta gacaatttat
    34921 accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc ttgactgact
    34981 cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa ttgcaagtcg
    35041 atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc tccatcagta
    35101 cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa gaagcagaac
    35161 atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat cccgaccaat
    35221 taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc aaatgtgcga
    35281 cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca gctttccatc
    35341 attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga atataaattc
    35401 gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac agaagaagtg
    35461 atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt ccgtctgaaa
    35521 tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct ttcagacatc
    35581 gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg tgactgggaa
    35641 gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga agctgaacga
    35701 gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca taatatgaaa
    35761 gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt gaaagaatac
    35821 cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa caaattagca
    35881 gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa agccgtcaaa
    35941 gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg gcaattcgaa
    36001 cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg ttgtacggca
    36061 cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac aagaaaagat
    36121 gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa cttagtaacc
    36181 aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa cctaggtgca
    36241 aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg aggcaccatg
    36301 ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa aatgatccac
    36361 aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga cggcggagaa
    36421 gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat ctctcatatc
    36481 gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc attcaatgag
    36541 atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat ctcagaagaa
    36601 caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga aatgatcgtc
    36661 atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt cgaactcaat
    36721 cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag gagctagaac
    36781 ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa tgaccagatt
    36841 ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct gttttcttag
    36901 gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt tcatgcccta
    36961 atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta gcatgtgtat
    37021 gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata tatcttaacc
    37081 aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa acatgttgct
    37141 ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt aatttccagt
    37201 tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa gctcttgttt
    37261 tcggagattg aattataact ttaccaaatt catctacagc aagagtttta ttgacatgta
    37321 gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca ctctttctca
    37381 tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca aaatctttaa
    37441 aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt ttctcatact
    37501 ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat tgcattgcaa
    37561 ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt tcatatttac
    37621 tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat agtttaagct
    37681 taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg attgttgaag
    37741 ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca aatgtcatat
    37801 caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca taatcttttt
    37861 gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca ccagttcgtg
    37921 gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc tttttgtact
    37981 ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt accactagat
    38041 ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg aaatcgtttg
    38101 ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat aagacatgtc
    38161 tttcaattga ataggtagcc attcatctac cattctttct agtagttctt ctatactgct
    38221 agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga ataattcttc
    38281 tatttttaag atattgaaaa gactaatttt ttcattttta actttattac aagtcttgtt
    38341 agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc atgtatctaa
    38401 attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt tatctggaaa
    38461 tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa tatattcttc
    38521 tacattgcca caaactaact cctctatact catatttccc aaatcagcaa ttcgggataa
    38581 tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt ttttgtcaat
    38641 taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt tactctttct
    38701 tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta ttggtggacg
    38761 ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg gtgttgtaaa
    38821 taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt atgtcgatga
    38881 actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt attttgagca
    38941 aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg agggggaaat
    39001 atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca agtgaaagag
    39061 ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac tctagagtat
    39121 atggactatc agcaggcaag tgaatatcta ggtgt
//
LOCUS       DACDEA010000024        38200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDEA010000024 DACDEA010000000
VERSION     DACDEA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.024"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..252
                     /locus_tag="IV136_001203"
     CDS             22..252
                     /locus_tag="IV136_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8465206.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            349..1650
                     /gene="murA"
                     /locus_tag="IV136_001204"
     CDS             349..1650
                     /gene="murA"
                     /locus_tag="IV136_001204"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8465207.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1663..1839
                     /locus_tag="IV136_001205"
     CDS             1663..1839
                     /locus_tag="IV136_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8465208.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1974..2270
                     /gene="yidD"
                     /locus_tag="IV136_001206"
     CDS             1974..2270
                     /gene="yidD"
                     /locus_tag="IV136_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8465209.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2454..2921
                     /locus_tag="IV136_001207"
     CDS             2454..2921
                     /locus_tag="IV136_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465210.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3006..3890
                     /locus_tag="IV136_001208"
     CDS             3006..3890
                     /locus_tag="IV136_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8465211.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4007..4240
                     /locus_tag="IV136_001209"
     CDS             4007..4240
                     /locus_tag="IV136_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8465212.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4328..4408
                     /locus_tag="IV136_001210"
     tRNA            4328..4408
                     /locus_tag="IV136_001210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4362..4364,aa:Tyr,seq:gta)
     gene            4415..4486
                     /locus_tag="IV136_001211"
     tRNA            4415..4486
                     /locus_tag="IV136_001211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4447..4449,aa:Gln,seq:ttg)
     gene            5001..5708
                     /locus_tag="IV136_001212"
     CDS             5001..5708
                     /locus_tag="IV136_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8465213.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5768..6412)
                     /locus_tag="IV136_001213"
     CDS             complement(5768..6412)
                     /locus_tag="IV136_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8465214.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6539..6826
                     /locus_tag="IV136_001214"
     CDS             6539..6826
                     /locus_tag="IV136_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465215.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6934..7254
                     /locus_tag="IV136_001215"
     CDS             6934..7254
                     /locus_tag="IV136_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8465216.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7335..8648
                     /locus_tag="IV136_001216"
     CDS             7335..8648
                     /locus_tag="IV136_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8465217.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8645..9364
                     /locus_tag="IV136_001217"
     CDS             8645..9364
                     /locus_tag="IV136_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8465218.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9492..10043
                     /gene="raiA"
                     /locus_tag="IV136_001218"
     CDS             9492..10043
                     /gene="raiA"
                     /locus_tag="IV136_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8465219.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10265..12799
                     /gene="secA"
                     /locus_tag="IV136_001219"
     CDS             10265..12799
                     /gene="secA"
                     /locus_tag="IV136_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8465220.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12925..14026
                     /gene="prfB"
                     /locus_tag="IV136_001220"
     CDS             join(12925..12987,12989..14026)
                     /gene="prfB"
                     /locus_tag="IV136_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8465221.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14131..14817
                     /gene="ftsE"
                     /locus_tag="IV136_001221"
     CDS             14131..14817
                     /gene="ftsE"
                     /locus_tag="IV136_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8465222.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14801..15694
                     /locus_tag="IV136_001222"
     CDS             14801..15694
                     /locus_tag="IV136_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465223.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15846..17294
                     /gene="cls"
                     /locus_tag="IV136_001223"
     CDS             15846..17294
                     /gene="cls"
                     /locus_tag="IV136_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8465224.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17631..18488
                     /locus_tag="IV136_001224"
     CDS             17631..18488
                     /locus_tag="IV136_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8465225.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18603..19523
                     /gene="pstC"
                     /locus_tag="IV136_001225"
     CDS             18603..19523
                     /gene="pstC"
                     /locus_tag="IV136_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8465226.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19523..20407
                     /gene="pstA"
                     /locus_tag="IV136_001226"
     CDS             19523..20407
                     /gene="pstA"
                     /locus_tag="IV136_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8465227.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20422..21228
                     /gene="pstB"
                     /locus_tag="IV136_001227"
     CDS             20422..21228
                     /gene="pstB"
                     /locus_tag="IV136_001227"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8465228.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21244..22002
                     /locus_tag="IV136_001228"
     CDS             21244..22002
                     /locus_tag="IV136_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8465229.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22015..22692
                     /gene="phoU"
                     /locus_tag="IV136_001229"
     CDS             22015..22692
                     /gene="phoU"
                     /locus_tag="IV136_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8465230.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22930..23961
                     /locus_tag="IV136_001230"
     CDS             22930..23961
                     /locus_tag="IV136_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8465231.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23958..24725
                     /locus_tag="IV136_001231"
     CDS             23958..24725
                     /locus_tag="IV136_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8465232.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24825..26393
                     /gene="liaX"
                     /locus_tag="IV136_001232"
     CDS             24825..26393
                     /gene="liaX"
                     /locus_tag="IV136_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8465233.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26693..27010
                     /locus_tag="IV136_001233"
     CDS             26693..27010
                     /locus_tag="IV136_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8465234.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27010..27360
                     /locus_tag="IV136_001234"
     CDS             27010..27360
                     /locus_tag="IV136_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8465235.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27527..28465
                     /locus_tag="IV136_001235"
     CDS             27527..28465
                     /locus_tag="IV136_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8465236.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28480..29310
                     /locus_tag="IV136_001236"
     CDS             28480..29310
                     /locus_tag="IV136_001236"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8465237.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29354..30379
                     /locus_tag="IV136_001237"
     CDS             29354..30379
                     /locus_tag="IV136_001237"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8465238.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30394..31332
                     /gene="galU"
                     /locus_tag="IV136_001238"
     CDS             30394..31332
                     /gene="galU"
                     /locus_tag="IV136_001238"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8465239.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31457..31897
                     /locus_tag="IV136_001239"
     CDS             31457..31897
                     /locus_tag="IV136_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8465240.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31900..32502
                     /locus_tag="IV136_001240"
     CDS             31900..32502
                     /locus_tag="IV136_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8465241.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32565..33668)
                     /locus_tag="IV136_001241"
     CDS             complement(32565..33668)
                     /locus_tag="IV136_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8465242.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33878..34879
                     /gene="ccpA"
                     /locus_tag="IV136_001242"
     CDS             33878..34879
                     /gene="ccpA"
                     /locus_tag="IV136_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8465243.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35234..35629
                     /locus_tag="IV136_001243"
     CDS             35234..35629
                     /locus_tag="IV136_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465244.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35629..35880
                     /locus_tag="IV136_001244"
     CDS             35629..35880
                     /locus_tag="IV136_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465245.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            35923..36318
                     /locus_tag="IV136_001245"
     CDS             35923..36318
                     /locus_tag="IV136_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465246.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36315..37571
                     /locus_tag="IV136_001246"
     CDS             36315..37571
                     /locus_tag="IV136_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP8465247.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37583..37906
                     /locus_tag="IV136_001247"
     CDS             37583..37906
                     /locus_tag="IV136_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465248.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37899..38165
                     /locus_tag="IV136_001248"
     CDS             37899..38165
                     /locus_tag="IV136_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465249.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12823 a         6497 c         8247 g        10633 t
ORIGIN      
        1 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
       61 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
      121 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
      181 ttagggtaca ctgcaagtaa ttttttctta gaagttatcg ctttatgccg aaatcttttc
      241 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
      301 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
      361 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
      421 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
      481 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
      541 gtagattttg atgaacaaaa gaatcaagta acaatcgatg cttcaagaca attagaaatc
      601 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
      661 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
      721 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
      781 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
      841 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
      901 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
      961 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
     1021 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
     1081 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
     1141 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
     1201 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
     1261 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgta
     1321 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
     1381 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
     1441 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
     1501 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
     1561 catatcaaac tacagcaatt aggtgcagac gttgaacgtg tagacatgga tcaaacttct
     1621 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
     1681 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
     1741 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
     1801 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
     1861 ctagtcgata tgaagtgagc catgaagcag aacatgacaa aagaaaacag aagggaacgt
     1921 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
     1981 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
     2041 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
     2101 cactaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
     2161 gcggtatcga ttatgtacca ttgacattcc aattaacgcg aaataagaaa gataaaaaag
     2221 aaccagatta taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa agaaacaatt
     2281 tggaaaatag cttcttatat tttgcaaaat tgtgagttat tgccgaagtg ttggattttg
     2341 aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
     2401 gaaaattatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
     2461 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
     2521 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
     2581 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
     2641 tagccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
     2701 agctttcaca actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca
     2761 gtgcggcgtt acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
     2821 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
     2881 taattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
     2941 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
     3001 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
     3061 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
     3121 aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
     3181 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
     3241 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
     3301 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
     3361 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
     3421 cgaaaggaca aatcaataaa ggggcacatc tttatggtgg agaatttggg ttgatgttct
     3481 taaatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
     3541 gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa agatgtcttt gaattagcag
     3601 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
     3661 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggtgttt
     3721 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
     3781 tcgatctgca tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc
     3841 ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga
     3901 gaaggtgcag cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa
     3961 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
     4021 taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
     4081 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
     4141 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
     4201 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
     4261 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
     4321 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
     4381 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
     4441 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
     4501 aagctctcgt tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc
     4561 tgctgattta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
     4621 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
     4681 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
     4741 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
     4801 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
     4861 aaattgtttc tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg
     4921 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
     4981 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
     5041 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
     5101 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
     5161 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcgcgagaaa
     5221 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcgcaaaatc
     5281 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
     5341 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
     5401 atgatgtccc tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca
     5461 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
     5521 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
     5581 tagaggtaaa acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
     5641 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
     5701 aaaaataaaa aacaggtgat ccctgattcc ttacttagaa ataaaagaga atcagaatca
     5761 cctgttttta tatatgatca gttaccagtt gctcgtgata agaaacagga ccctcttcaa
     5821 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt
     5881 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
     5941 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
     6001 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagacagcat
     6061 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
     6121 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgccag
     6181 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
     6241 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac
     6301 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
     6361 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
     6421 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
     6481 acggttgcgc aatcgctgta gtatgcgata ataaagacaa aggtaggtgg atctggggat
     6541 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
     6601 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
     6661 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
     6721 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
     6781 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
     6841 gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt
     6901 ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta
     6961 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
     7021 aaaggaatcg actcggatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
     7081 aataaagatg tagatgtact gttattagga cctcaagttc gctttatgaa agctgatttt
     7141 gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca tcaatatggc tgattatggt
     7201 atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa
     7261 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
     7321 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
     7381 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
     7441 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gatggacagc
     7501 ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac
     7561 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
     7621 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
     7681 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
     7741 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
     7801 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
     7861 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
     7921 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
     7981 cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac
     8041 gattcatcta cttaagtgcc acacctgata aaaagttact aaaggaaatc aagcagacgt
     8101 ttgacatcca caaacttgct ttgcgctttc atcggcgttt acttcctgaa cccgtcttgg
     8161 ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc
     8221 ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga
     8281 tgatgcatcg tttgaaagcc acaattcaag aggctcttcc gagatatgag atcacagatg
     8341 tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg
     8401 acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta
     8461 tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa
     8521 gagccgatcg taaagggcct tatacgaaca gtcaagtata ctttttctat gaagaaaaaa
     8581 ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga
     8641 tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat tcagcgagat
     8701 tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca
     8761 tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag
     8821 tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt gactgtcaag aatggaagaa
     8881 gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa
     8941 ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata
     9001 taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc
     9061 tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag
     9121 aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac
     9181 gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat
     9241 acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca
     9301 cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt ctttagcaag
     9361 atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat
     9421 aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag
     9481 gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga
     9541 ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg
     9601 atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa
     9661 cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa
     9721 gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta
     9781 atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tactgcttta ttcaatgaag
     9841 aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga
     9901 aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta
     9961 tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat
    10021 acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag
    10081 ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata
    10141 aattatgaag ttcttacttt aatacgctta agatatttca attatctata actaatgata
    10201 aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca
    10261 acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt
    10321 agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga
    10381 acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga
    10441 tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattggggtt
    10501 atatccacac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga
    10561 aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct
    10621 gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac
    10681 ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa
    10741 atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga
    10801 attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg
    10861 tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac
    10921 gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa
    10981 cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat
    11041 cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata
    11101 cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat
    11161 catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt
    11221 cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc
    11281 taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa
    11341 ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga
    11401 agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc gtccggttat
    11461 ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc acgctgtcgt
    11521 gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt
    11581 tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct
    11641 gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc
    11701 tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt
    11761 aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga
    11821 taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac agttctactt
    11881 gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaat ctttcttaga
    11941 tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt
    12001 agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca
    12061 atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat
    12121 catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg
    12181 tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga
    12241 ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata tcgaaggaaa
    12301 aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa
    12361 agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg
    12421 tgttgtggac tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat
    12481 cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa
    12541 gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc
    12601 tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga
    12661 agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa
    12721 caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca
    12781 tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag
    12841 aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa
    12901 agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa
    12961 atgcaggaaa aggtcacaag cttcaggagg tctctttgac ttagaccagc tggaagaaga
    13021 cattgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgata gcgaagctgc
    13081 tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact tatgatcaat tccattctct
    13141 tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg ttgcaggaag aacctgatgc
    13201 agccatgcaa gaagaagcag aaacacgtat caatgcgcta gatgaaaaaa tgaagacata
    13261 tgaattatct atgttgttag atggtcctta tgatcgaaac aacgccatcg ttgaattgca
    13321 tccaggggct ggcggaacgg agtcccaaga ttggggcagt atgcttttac gtatgtatac
    13381 acgctgggca gagcagcatg ggtatcaagt agagacacta gattatcaag caggtgatga
    13441 agcaggaatc aagagtgtga ccctactgat caaaggacac aacgcatttg gttatctgaa
    13501 atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct tttgactcag cgaaacgacg
    13561 tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga
    13621 aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca
    13681 catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc
    13741 tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc
    13801 taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga
    13861 acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc catattctat
    13921 ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga
    13981 ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac ccattgttaa
    14041 attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct
    14101 aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag
    14161 tactcaaatg gtacgaccgc tatccgcaat ctctcaattg tcatcgaaca aggagagttt
    14221 gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga
    14281 gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa
    14341 aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta
    14401 ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa
    14461 cctcgggata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa
    14521 gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg
    14581 gcaatagtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca
    14641 gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc
    14701 gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc
    14761 gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac
    14821 ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt
    14881 tgcttcagtc agtgcggtaa cgatcacatt gactttagtc ggtgtattca tggcagttat
    14941 catgaacgca actaaattag ctcaagatat agaaggaaac gttgatgtct ctgtattcgt
    15001 agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga
    15061 tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga
    15121 aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt
    15181 agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa
    15241 gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg
    15301 tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct
    15361 catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg
    15421 tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat
    15481 cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg catggttttt
    15541 ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc cacaaaatat
    15601 cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc
    15661 gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata aaatgagaaa
    15721 gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc
    15781 ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg
    15841 aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc
    15901 tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatgggtgc
    15961 tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga
    16021 tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa
    16081 ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag
    16141 tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta
    16201 aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata
    16261 tcaagcaggc agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag
    16321 gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc
    16381 ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag
    16441 caggcggaga agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca
    16501 attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt
    16561 tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc
    16621 atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga
    16681 attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc attcactcta
    16741 ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca
    16801 aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt
    16861 attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa
    16921 aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt
    16981 ctttcgctgc agagctaatc gagtatggag caaggatcga aacatatgag aatggattta
    17041 ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg
    17101 atgttcgttc atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg
    17161 cttcacgtgt aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag
    17221 aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt
    17281 cgccgctcct ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt
    17341 ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga
    17401 gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata
    17461 tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta atgagatagt
    17521 tttttttaca attatgagaa tttattttgt tatttaatga ttttgattcg ttttcatcaa
    17581 gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac
    17641 ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt tcaaagtgga
    17701 ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa
    17761 cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg
    17821 gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt
    17881 tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag
    17941 ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa
    18001 acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc
    18061 agccagtagt tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat
    18121 gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc
    18181 gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg
    18241 atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga
    18301 acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga
    18361 caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc gttggagaac
    18421 taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta
    18481 aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc
    18541 aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat
    18601 ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc
    18661 gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt
    18721 tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg
    18781 attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg
    18841 taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg
    18901 ccacgccatt tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa
    18961 cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt
    19021 ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg actgggtttg
    19081 gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga
    19141 ctgttgatgc attaaaggca gtgccacggc actatcggga agcttcatta gcgcttggtg
    19201 ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa
    19261 cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca
    19321 tcggtaatgc agcattgatg ccaacaggtc ttactactcc tgcttctaca ttgacgtcca
    19381 tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt
    19441 cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg
    19501 ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt
    19561 tttatatata gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt
    19621 agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca
    19681 agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat
    19741 gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa
    19801 aaactggcta acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt
    19861 cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat
    19921 tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga
    19981 ggagtcttta caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc
    20041 acgctgggaa acagtgataa aagtggtggt tccagaagcg acaccaggga ttttgactgg
    20101 tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg
    20161 acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat
    20221 cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg
    20281 cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga ttattgctgt
    20341 tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc
    20401 agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc
    20461 atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac
    20521 aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt
    20581 ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg
    20641 atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct
    20701 caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg
    20761 ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac
    20821 aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa
    20881 gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta
    20941 tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt
    21001 gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac
    21061 tatacgatca tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact
    21121 gcctttttct atttaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga
    21181 ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc
    21241 aacatgacaa aagagattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa
    21301 gcactaaaag ggatcgatct aagcttcaat caaggggaaa tcactgcgat gatcggtcct
    21361 tctggttgcg gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg
    21421 aatgtaacga tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca
    21481 gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct
    21541 ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa
    21601 gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa
    21661 gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc
    21721 gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt
    21781 gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg
    21841 atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc
    21901 tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat gaatccagaa
    21961 aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta
    22021 cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg
    22081 atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta
    22141 gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag
    22201 aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt
    22261 acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct
    22321 aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc
    22381 gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca
    22441 gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat
    22501 cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga
    22561 acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc
    22621 tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat
    22681 gagattttct aaactgaagc tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat
    22741 cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaaatagc
    22801 tcctcatatt tttcgtttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt
    22861 tattcgggaa agggtttgtg acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa
    22921 ggataaagaa tgcaaaaaaa aattagcatc atcactatat tttttatttg tgtgcttact
    22981 tcatgggtta ttcagcatca agccgcttta tatgaagtac ctgtcatcga gatagaaaaa
    23041 gtacgaataa aagaggatca tcaattgatt acaggaaaac tgatgaacac ggatcaaaaa
    23101 ggagaatcga tttatttatc ggttccttat caaaaaaatc aaatagaaac agcaaatata
    23161 aaagcgagac aaatgtatct agtccaaatg catggagatg aatggcagct agtagcaaaa
    23221 aaaaatgatg gctggctttt cttcttcaca agtatcttca ttggcgccat gttgcttatt
    23281 ggtggaaaac agggtctatt tgctttagga agtctcatca tcaatatcgt tttactttta
    23341 cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaatat atctatcctg
    23401 ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa tagaagcagt
    23461 ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg
    23521 acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga
    23581 ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat
    23641 accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca
    23701 gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc
    23761 aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga
    23821 aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc
    23881 attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt ttttctgact
    23941 gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg
    24001 tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag
    24061 ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg
    24121 tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa
    24181 aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc
    24241 ctatcactga ttttttagca atccaaggtt ttacatcggt agaactagaa gaattatctg
    24301 gcttagataa gactttagcg attgatttta gattacttac gagatcgttg cttctgatta
    24361 gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat
    24421 atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta
    24481 aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag
    24541 cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat
    24601 catttgttct agaatatacg atggctattc ttaccacatt gtccgcacta cttgttcttc
    24661 ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg
    24721 agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg
    24781 taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac
    24841 gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag
    24901 aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg
    24961 ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa aacggcgaag
    25021 aagaaatttc tgagccagtt gatccaaagg aaagagaaag acaagatcaa gaaaacttag
    25081 aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag
    25141 taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa gcattgatgc
    25201 agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaga
    25261 cgttagaagc agaaatcaaa gaattagaag ctagtggcga tacactgtta gaagaacaat
    25321 tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag aaaaaagaaa
    25381 cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca gatacactga
    25441 atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa
    25501 cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat gtcagcttac
    25561 gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa gctcctgcag
    25621 caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca
    25681 acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct gaaccatttg
    25741 aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc ttccaaattc
    25801 caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta tacgaccacg
    25861 cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct aaagatattt
    25921 atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg atgctggaag
    25981 tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatcg
    26041 aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct gtgtctttgg
    26101 taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt
    26161 cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa ggacatgcta
    26221 aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa
    26281 aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa atcgcgcaag
    26341 tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt
    26401 gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag cttcccctat
    26461 tttacaaaat tgtgccttat tgccgatagt ctatctcttg aacaccgttt accaagagat
    26521 tatgaacctg ccaattatcg ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt
    26581 tttatgaata tggtaatttg tgaccgagaa ttttgcagaa agacaccgat taagaagaag
    26641 gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca tgatgaaaaa
    26701 aaagttgaca aaatctaata aaaatgttgt tttgacaggg actttagcag gaataggaga
    26761 atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga gcttagttgc
    26821 ttttggaagt ccaattattt tatacatttt attagctttg attatcccat ctgcgcgttc
    26881 tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt atcgtgaaca
    26941 aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg acgaatggag
    27001 tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt catatcattg
    27061 gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt tcttgccagc
    27121 ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact gtccctgccc
    27181 tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat tcttaaaatg
    27241 acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc agcgttgtta
    27301 gtagctatct tgatgtcgct tgtcaatgta attgtcagcg aacataacag tagaaaataa
    27361 aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga atgcgttttc
    27421 tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca
    27481 atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt
    27541 aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg acgagaaaag
    27601 cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt tgactggtta
    27661 ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa tcagttttgc
    27721 ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat
    27781 gcccgcattt attatttcaa gaggattacc agctccagaa gaaatgatcc aggcagcaaa
    27841 tgaaaatggg atagctgtac tgcgttctcc catctcaact tctcgtttat taggagaatt
    27901 atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg ttttagtgga
    27961 tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc
    28021 gttggagttg atcaaacgcg gccatcgatt gatcgcagac gatcgagtag atgtttatca
    28081 gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc tgattgaaat
    28141 ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc
    28201 tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga aatacgaccg
    28261 tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat
    28321 ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg
    28381 tgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat
    28441 tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca aatcaatcga
    28501 gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat cgtatcaggt
    28561 atcattatcg ctatgtggct tagcagcaga gaggctgttc gggtaggatt aaaagcagat
    28621 gatgtgacag attttatgtt gtggggattg cctatctcca ttattggcgc tcgcttatat
    28681 tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt caatatccgt
    28741 tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct gtattttttt
    28801 actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc aagtgtatta
    28861 ttagcacagg caatcggtcg ttggggaaat ttcatgaacc acgaagcata tggaccagac
    28921 acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga taatatgtat
    28981 atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag tttgattgga
    29041 tttattttgc ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt
    29101 ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt acgtacagac
    29161 agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt gttatttgtc
    29221 ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc
    29281 tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg aaaaaaattt
    29341 caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt ggggtactgc
    29401 tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac atcatgcttc
    29461 acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag atcattatct
    29521 acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg cagatgcggt
    29581 gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag tgcctaaatt
    29641 gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggaa cacataaaag
    29701 gatctctgac atcttgttag aggaaatccc agcagaaaaa agaaaagctg ttgttgttct
    29761 ttctggacca agccatgcag aagaagtagc aaaacatgac atcactacga tcacagctgc
    29821 aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata attattttcg
    29881 gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga aaaatattat
    29941 tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa aggcagcaat
    30001 catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct
    30061 gacctttatc ggattaagcg gaataggcga tctagtcgtt acttgcacaa gtgttcattc
    30121 taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag aggtgctagc
    30181 caatatggga atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg aactagccca
    30241 aatgatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa
    30301 agaaatcaaa acagccgcaa aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt
    30361 ctcattaaca attttttgaa ggagcgaatg tatatgaaag ttaaaaaagc agttattcca
    30421 gcagcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa ggaaatgttg
    30481 ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa atcaggtatc
    30541 gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca
    30601 aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt gaaattagta
    30661 gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa gggtttagga
    30721 catgccgttc ttcaagcaag agcctttatt ggaaatgaac catttgtcgt tatgctaggc
    30781 gatgatataa tggaagacga agtaccattg acacagcaat tgatcgatga ctatgagcgt
    30841 acacacgctt ctacgattgc cgttatgcaa gttcctcacg atgaaacttc aaaatacgga
    30901 atcatcaacc caggggaagt actggaagat gggttataca atgtcaaaaa ctttgttgaa
    30961 aaaccagatc caagcaaagc accaagtgat ttagctatta ttgggcgtta cttattgact
    31021 ccagaaatct ttgatgtatt atcagaacaa caaccaggtg ctggaaatga gatccaacta
    31081 actgacgcaa tcgatacgtt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt
    31141 aaacgttatg acgttggtga caaatttggt tttatgaaaa caagtattga atatggtctg
    31201 acacatccag aagtaggggc acctttgcgt gaatacatca tgtctttagg taaaacatta
    31261 gaagcagaag gaagcaagat caatcataat ccaaaaggaa taacagaaat caaagaagaa
    31321 aagaaaaaat aatctttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg
    31381 aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca
    31441 agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag caatcgcctt
    31501 tgcgatactt gtcgttttca tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa
    31561 agtagaccag actgtcgcag aagcaaatac gacaattgag atcgtcacac aagacgtaga
    31621 tcttttatct agacaagtag aaggcttgct agtcaaaagt aatgagctgc tcttggatat
    31681 caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag
    31741 tgtatcggaa cttaatcatt ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa
    31801 aacgacagct aaagcgactg ttgccggaaa agtcggtgga acagcgatga aattttttaa
    31861 aaacaaaaaa catactgaaa cgacaggagg aaaataagaa tggctaaaaa aggtggattt
    31921 ttattaggtg cagtagttgg aggtacagcc gcagcaatcg ccgcattatt gttagcaccc
    31981 aaatctggta aagaacttcg ggatgaattg gctgctcaag cagatgattt gatggacatg
    32041 gcttcagatt acacagatat ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa
    32101 gaaaaagcgc aaggtctttc tcaacaagca ggggatctag caggcaatgt taagcagaag
    32161 gtaagtgaat cagcagagga agcaggaagt gaatcagatg atatcttgtt aaacctcaaa
    32221 gaacaatcag ctgatttatc taatcgtttc aaaaaaacag cagaagatct gaaaaagcca
    32281 gcagacgaat taggtagtat tgctaaagat acttcagaag atatcatgat cgatgtaaaa
    32341 gatgctgcag ataaagcagg agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt
    32401 gcagatgaca caaaagaaat gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa
    32461 gaaatgaaag atacaatgac agattcgact accaatcact aagattactc atttctagaa
    32521 taaaaaaggt gagagcggac cagctctcac cttttttatt ttcattactc taaaacaagt
    32581 aattcttttg gtgtagtcgt gaatggcaca caaccatcac ttgtcacata cacacaatct
    32641 tcgatacgta cccctacttt ttcaggaata tagattcctg gttcaagaga gaagcacatc
    32701 ccttcttcaa tcactaagtc gtttccttct acaagagaag ggaattcgtg gacggttgtt
    32761 ccgattccgt gacctaaacg gtgattgaaa tattcgccgt agccatagct gttgatcacg
    32821 tttcgtgcga tttgatcaag ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg
    32881 gcaagctgtg cttcaagagt gatgttatag attttttctt ggaactcagt tggtttttga
    32941 taagcaactg tacgagtagc atcactgcaa tagccattcc aaataacgcc aagatcgaat
    33001 aacactagtt catttggtga aaccttcgtg cttcctggtg taccatgagg gcttgcagca
    33061 tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat
    33121 tggtattcga tttctgcgat aattgcttgt tcagttacgc cttcttttac tgttttgaag
    33181 ccaatttcaa aggcaacatc ggcccattta ccagcttcga tcagtttgtc tttttcttct
    33241 accgttttga tcagttgcag acgctggata atagaagtta gatcggtgta agaatctgta
    33301 cctgtcaaga tatcagagat agcatcgaag cgatcgacag tcagagcatt cttttcaata
    33361 cctacattac caggttgccc atagcgtgcg cgaagctctt ttccgatgat ctcccaagga
    33421 ttttcgctgt ctaagtagcc acgtacatcg tacgcccagc tgctgttttg agcatcttca
    33481 acttccagag caggagtgaa aaggaaagga tcttgttcca gagggataaa aagagctagt
    33541 acacgctcgt gaggatcact ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg
    33601 atataagcca attctacttg gtgttgtgcc atccattgac gtagttcatt gatttttacg
    33661 ttattcattt ggcaacctac tttctaaatt aattttgtat tcaataattg taccactgat
    33721 tgattaaaag accaacgttc ttaaaactga aaaaagtaaa aattttcatg aaaacagaga
    33781 taaaacggtt gcaattgttg agattatttg atatcctatt ttagaaatgt aagacgtatt
    33841 ttcagaaaac atataaaata tgaataggag aacacaaatg gaaaagcaaa caatcactat
    33901 ctatgatgta gcgagagagg caaatgtttc gatggctact gtttcacgtg tcgtaaatgg
    33961 caatccaaac gtcaaaccag caacaagaaa aaaagtcttg gaagttatcg atcgcttgga
    34021 ttatcgtcca aatgcagtcg cacgtggctt ggcaagtaag aaaacaacaa cagttggggt
    34081 aatcattcct gatgtcagca atatgttctt tgcctcttta gcacgtggta tcgacgatgt
    34141 ggcaacaatg tacaaataca atatcatctt agcaaactca gatggcaacg atcaaaaaga
    34201 agtcaatgta ttgaataatt tattggctaa gcaagtggac ggcgtgatct ttatgggtca
    34261 ccatatcaca gacgaaatcc gtggagaatt ttcacgttca aaaacgcctg ttgtactagc
    34321 tggttccatt gatccagatg aacaagtagg tagcgtaaac attgattatg tgtcagcaac
    34381 aaaagatgca gtaaataaat tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg
    34441 tgcgctgatt gatcctatca atggtcaaaa ccgtctaaaa ggctataaag aagcattaag
    34501 cgaaaacaat ctaccatata gcgaaggatt gatttttgag gcacaataca actttaaaga
    34561 tggtctgtct ctagttgacc ggatccataa tagcggtgca acagctgtct atgtttcaga
    34621 cgatgaatta gccattggta tcttggatgg tttattagac cgcggcgtaa aagtaccaga
    34681 agaattcgaa atcatcacaa gcaacaattc tttactgaca gaagtagctc gtcctcgttt
    34741 gacaagtatc acacaaccat tatacgattt aggagcggta tccatgcgcc tattgacgaa
    34801 aatgatgaat aaagaagaag tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga
    34861 aaaaggatca actaaataat aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa
    34921 cttctggaac aacgtttata cgagaaaggg ggttatgaca aaattttgtc ataacccctt
    34981 tttttttgtg actttaaaaa aagagaaaat ggcggaaaca aaccttacgt ccatcgtata
    35041 cgacacaatc tgtgcgtaga tgctacattt tgttcactac tatcaaaatt actttaaaat
    35101 gaaaacgtga taaaaaagaa agtagaaaag tagataagta actctaaaaa atccaaaaaa
    35161 atccaggctc gattgcgtac ttatcttaaa ggagaaacaa tccatcatat ataacccgaa
    35221 aggagacttc tatttgccta agaaaagacg aaagaccata gtcatcattt tgaccattct
    35281 tactatagga atcggaggga aattttacat ggacaaaaga gaaatgcaaa gacagaaaga
    35341 tttactcgca gtagaaaaac agtccgtcaa agttctgaaa aatacctttg cggatatcaa
    35401 ggaagtgaag attgaaaaat ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat
    35461 tctaatgact aataaacaag atcaatcgat ttatttttct tatagttttt ggaaagaaag
    35521 aaatgagata ggaagttacg gaatagttga tgaaaaaaag caaaaagaag gaaatacgct
    35581 caataaagtt aaagtaactt actctaatgg aaatgaggaa agtatttaat gattactgac
    35641 aaacaatata gtgaattaag tgatgctgtt tactggctag atcctaaaca tagagactat
    35701 gtacctgaaa tgcaagaaaa tttaagtttt aaaattaacg gcactatgta taaaatccta
    35761 aaaatcaaaa actccttcga cggcatgcag gcgatggctg tagcgccaat cgtccatagc
    35821 aaattggaga aaaatttcaa aaataaaaaa attcctgcaa actttcgcgt acttaaataa
    35881 tgaaggtgtc aatacttaca taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga
    35941 aaactcagct tagccatttt attaatcgtc atattaggga tcggagggaa agtctatatg
    36001 gacaaaagag aagcacaaag gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa
    36061 gttctgaaaa atacctttgc ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac
    36121 cctgttaccg gaagttatgg tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt
    36181 tattttgatt atagtttttg gaaggagaga aatgaacttg gtggttttgg cattgagaac
    36241 agagcggtac aaaagacagg agtcactata aaaagagtaa aagtaatttt ttctaacgga
    36301 caggaggagt tagtatgaca ataaacgata agcattacaa tgatattagt gagaaggttt
    36361 attggctaga tcccaaatat ccccgctaca atgagggata caaaaagaac tctgttaaag
    36421 aatttgctgg tatggaattt caaatcctac aaatcaaaga ctcattggac ggcatgcagg
    36481 cgatgtctgt agcgccaatc aagaacggta aagtggatct ttctcaagta gtgattgctt
    36541 ttgcaggaac gaacttaagt gattggaaag atttggaaac ggatgcaaga agtatcatga
    36601 atctctacgg gaatagacca ggagcaagtt tttctgaaaa agcaacaaca aacaagattt
    36661 tatccgttag tggattgaaa acagcggggc aaatcaattc agcgattgca tttgctaatg
    36721 aagtaaaaaa ggaatatgga gaagctgcta tttctactac cggccattca ttaggtggat
    36781 tcttagcatt atatgtagca gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg
    36841 acccgtatga tctcttatca ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc
    36901 tcaccaatta ccgaaatcac gccgatttta tcggtaattt tggtggtaat ggcacaggtg
    36961 ctgaagttcg gatcagtatg gaaacagcat tcttgaatct gcttgcttcc cacagtttaa
    37021 cctcttggaa gtttgatgaa aatggaaagc tgattattcc tgaaaatgaa aacaatgtta
    37081 aagctcgaaa acaacagaaa gaaaaccaag cgacccagtt gttttcctat cagttgcaag
    37141 aactgaacaa catacgtaaa aaactacgat taagtggcgg ccagctgaca gttaatgaac
    37201 aaatttactt agatgacaca caagctaggt tagtagtgga aaagttatcg gcaaaaatga
    37261 aaagcgctat ggaagaagtt atccgcatca atcaaagagg aatttttgag ctagagaaaa
    37321 aatggcaaga aggcttgcgg caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa
    37381 tcatagaagc attagctagt gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag
    37441 aagtctatct gctacgaatc aataaggcaa gacgtatggg ggaggccttt gatacattgg
    37501 cgaaggagat ccaagacaaa atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc
    37561 aattcaatta aaggagaagt agatgaaact agaagaattg gaaagagatt atcgaagtga
    37621 acgaagacaa ttagaagctc aagaagatga attgatccac tttaagaatc aaggattacg
    37681 attgatggag caagaggagc agcgactgca tcatgtatta aagaacttag ctttagataa
    37741 tgagcctctg tttcaagcaa gacgaagtta ttcgaattat caggagcatt atcaacaagc
    37801 cctacaagta gaacgacgaa aactctatca gcagcaagat gaactagaac gttcctatcg
    37861 tcaaaaaatc agacaaataa ctatggagaa atcatcttat gaatgaattt ttaatgcaat
    37921 tactaaaaga attagagaaa aatctacatc gatcaaatgt acgttcagtt ggtatgggga
    37981 tatctcagca aatttgggca gaaaaacgat tgacagatca aaactaccaa atgactgcag
    38041 aagaagctgc tcaagaattt tctaaccgac atgtacactt gaaaggatca gtaaaagggc
    38101 aatttgcaga aaaactaact gaattattcc aaagacaaga aaagaaatta aaacagtttc
    38161 gttagtaaag ttatatagaa aaattttgtg aaaatggttt
//
LOCUS       DACDEA010000025        36326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDEA010000025 DACDEA010000000
VERSION     DACDEA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.025"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            356..631
                     /locus_tag="IV136_001249"
     CDS             356..631
                     /locus_tag="IV136_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465250.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            644..2722
                     /locus_tag="IV136_001250"
     CDS             644..2722
                     /locus_tag="IV136_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8465251.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2882..4768
                     /locus_tag="IV136_001251"
     CDS             2882..4768
                     /locus_tag="IV136_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8465252.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4780..5727
                     /locus_tag="IV136_001252"
     CDS             4780..5727
                     /locus_tag="IV136_001252"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8465253.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5747..6274
                     /locus_tag="IV136_001253"
     CDS             5747..6274
                     /locus_tag="IV136_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8465254.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6337..6990)
                     /locus_tag="IV136_001254"
     CDS             complement(6337..6990)
                     /locus_tag="IV136_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8465255.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7122..7964
                     /locus_tag="IV136_001255"
     CDS             7122..7964
                     /locus_tag="IV136_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8465256.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8122..9018
                     /locus_tag="IV136_001256"
     CDS             8122..9018
                     /locus_tag="IV136_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8465257.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9021..9734
                     /locus_tag="IV136_001257"
     CDS             9021..9734
                     /locus_tag="IV136_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8465258.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9754..10272
                     /gene="msrA"
                     /locus_tag="IV136_001258"
     CDS             9754..10272
                     /gene="msrA"
                     /locus_tag="IV136_001258"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8465259.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10269..10496
                     /locus_tag="IV136_001259"
     CDS             10269..10496
                     /locus_tag="IV136_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8465260.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10648..11199
                     /gene="lepB"
                     /locus_tag="IV136_001260"
     CDS             10648..11199
                     /gene="lepB"
                     /locus_tag="IV136_001260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8465261.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11282..11824)
                     /locus_tag="IV136_001261"
     CDS             complement(11282..11824)
                     /locus_tag="IV136_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8465262.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12061..12459)
                     /locus_tag="IV136_001262"
     CDS             complement(12061..12459)
                     /locus_tag="IV136_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8465263.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12719..13579
                     /gene="ylqF"
                     /locus_tag="IV136_001263"
     CDS             12719..13579
                     /gene="ylqF"
                     /locus_tag="IV136_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8465264.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13572..14339
                     /locus_tag="IV136_001264"
     CDS             13572..14339
                     /locus_tag="IV136_001264"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8465265.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14395..15252
                     /gene="dprA"
                     /locus_tag="IV136_001265"
     CDS             14395..15252
                     /gene="dprA"
                     /locus_tag="IV136_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8465266.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15374..17452
                     /gene="topA"
                     /locus_tag="IV136_001266"
     CDS             15374..17452
                     /gene="topA"
                     /locus_tag="IV136_001266"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8465267.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17421..18797
                     /gene="trmFO"
                     /locus_tag="IV136_001267"
     CDS             17421..18797
                     /gene="trmFO"
                     /locus_tag="IV136_001267"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8465268.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18995..19900
                     /gene="xerC"
                     /locus_tag="IV136_001268"
     CDS             18995..19900
                     /gene="xerC"
                     /locus_tag="IV136_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8465269.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19937..20485
                     /gene="hslV"
                     /locus_tag="IV136_001269"
     CDS             19937..20485
                     /gene="hslV"
                     /locus_tag="IV136_001269"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8465270.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20499..21899
                     /gene="hslU"
                     /locus_tag="IV136_001270"
     CDS             20499..21899
                     /gene="hslU"
                     /locus_tag="IV136_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8465271.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21917..22708
                     /gene="codY"
                     /locus_tag="IV136_001271"
     CDS             21917..22708
                     /gene="codY"
                     /locus_tag="IV136_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8465272.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22790..23665
                     /locus_tag="IV136_001272"
     CDS             22790..23665
                     /locus_tag="IV136_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8465273.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV136_001273"
     CDS             complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV136_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8465274.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24562..24996
                     /locus_tag="IV136_001274"
     CDS             24562..24996
                     /locus_tag="IV136_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8465275.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25162..27207
                     /gene="parE"
                     /locus_tag="IV136_001275"
     CDS             25162..27207
                     /gene="parE"
                     /locus_tag="IV136_001275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8465276.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27219..29669
                     /gene="parC"
                     /locus_tag="IV136_001276"
     CDS             27219..29669
                     /gene="parC"
                     /locus_tag="IV136_001276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8465277.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29946..32177
                     /gene="pflB"
                     /locus_tag="IV136_001277"
     CDS             29946..32177
                     /gene="pflB"
                     /locus_tag="IV136_001277"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8465278.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32421..33182
                     /gene="pflA"
                     /locus_tag="IV136_001278"
     CDS             32421..33182
                     /gene="pflA"
                     /locus_tag="IV136_001278"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8465279.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33267..34193
                     /locus_tag="IV136_001279"
     CDS             33267..34193
                     /locus_tag="IV136_001279"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8465280.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34375..35268
                     /locus_tag="IV136_001280"
     CDS             34375..35268
                     /locus_tag="IV136_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8465281.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35402..35527
                     /locus_tag="IV136_001281"
     CDS             35402..35527
                     /locus_tag="IV136_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465282.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35661..36293)
                     /locus_tag="IV136_001282"
     CDS             complement(35661..36293)
                     /locus_tag="IV136_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465283.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12747 a         6225 c         7632 g         9722 t
ORIGIN      
        1 ttaatcatac tagagaacga tatgtttttt aatacctaaa ataaaaatgg ggctgaagaa
       61 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
      121 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      181 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      241 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      301 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      361 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      421 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      481 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      541 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      601 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      661 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      721 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      781 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      841 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      901 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      961 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
     1021 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1081 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1141 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1201 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1261 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1321 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1381 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1441 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1501 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1561 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1621 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1681 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1741 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1801 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1861 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1921 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1981 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     2041 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2101 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2161 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2221 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2281 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2341 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2401 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2461 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2521 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2581 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2641 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2701 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     2761 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     2821 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     2881 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     2941 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     3001 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     3061 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     3121 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     3181 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     3241 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     3301 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     3361 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     3421 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     3481 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     3541 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     3601 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     3661 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     3721 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     3781 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     3841 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     3901 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     3961 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     4021 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     4081 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     4141 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     4201 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     4261 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     4321 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     4381 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     4441 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     4501 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     4561 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     4621 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     4681 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     4741 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     4801 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     4861 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     4921 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     4981 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     5041 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     5101 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     5161 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     5221 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     5281 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     5341 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     5401 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     5461 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     5521 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     5581 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     5641 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     5701 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     5761 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     5821 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     5881 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     5941 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     6001 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     6061 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     6121 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     6181 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     6241 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     6301 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     6361 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     6421 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     6481 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     6541 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     6601 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     6661 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     6721 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     6781 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     6841 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     6901 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     6961 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     7021 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     7081 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     7141 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     7201 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     7261 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     7321 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     7381 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     7441 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     7501 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     7561 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     7621 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     7681 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     7741 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     7801 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     7861 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     7921 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     7981 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     8041 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     8101 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     8161 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     8221 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     8281 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     8341 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     8401 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     8461 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     8521 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     8581 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     8641 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     8701 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     8761 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     8821 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     8881 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     8941 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     9001 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     9061 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     9121 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     9181 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     9241 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     9301 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     9361 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     9421 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     9481 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     9541 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     9601 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     9661 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     9721 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     9781 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     9841 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     9901 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     9961 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
    10021 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    10081 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    10141 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagactatta taaaaagcat
    10201 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    10261 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    10321 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    10381 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    10441 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    10501 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    10561 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    10621 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    10681 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    10741 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    10801 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    10861 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    10921 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    10981 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    11041 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    11101 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    11161 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    11221 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    11281 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    11341 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    11401 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    11461 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    11521 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    11581 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    11641 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    11701 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    11761 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    11821 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    11881 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    11941 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    12001 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    12061 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    12121 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    12181 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    12241 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    12301 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    12361 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    12421 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    12481 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    12541 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    12601 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
    12661 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
    12721 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    12781 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    12841 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    12901 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    12961 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    13021 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    13081 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    13141 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    13201 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    13261 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    13321 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    13381 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    13441 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    13501 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    13561 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    13621 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    13681 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    13741 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    13801 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    13861 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    13921 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    13981 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    14041 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    14101 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    14161 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    14221 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    14281 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    14341 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    14401 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    14461 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    14521 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    14581 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    14641 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    14701 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    14761 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    14821 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    14881 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    14941 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    15001 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    15061 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    15121 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    15181 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    15241 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    15301 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    15361 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    15421 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    15481 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    15541 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    15601 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    15661 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    15721 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    15781 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    15841 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    15901 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    15961 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    16021 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    16081 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    16141 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    16201 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    16261 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    16321 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    16381 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    16441 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    16501 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    16561 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    16621 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    16681 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    16741 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    16801 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    16861 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    16921 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    16981 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    17041 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    17101 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    17161 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    17221 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    17281 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    17341 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    17401 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    17461 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    17521 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    17581 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    17641 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    17701 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    17761 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    17821 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    17881 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    17941 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    18001 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    18061 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    18121 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    18181 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    18241 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    18301 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    18361 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    18421 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    18481 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    18541 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    18601 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    18661 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    18721 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    18781 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    18841 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    18901 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    18961 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    19021 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    19081 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    19141 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    19201 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    19261 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    19321 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    19381 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    19441 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    19501 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    19561 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    19621 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    19681 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    19741 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    19801 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    19861 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    19921 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    19981 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    20041 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    20101 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    20161 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    20221 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    20281 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    20341 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    20401 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    20461 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    20521 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    20581 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    20641 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    20701 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    20761 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    20821 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    20881 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    20941 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    21001 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    21061 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    21121 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    21181 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    21241 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    21301 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    21361 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    21421 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    21481 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    21541 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    21601 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    21661 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    21721 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    21781 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    21841 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    21901 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    21961 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    22021 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    22081 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    22141 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    22201 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    22261 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    22321 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    22381 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    22441 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    22501 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    22561 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    22621 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    22681 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    22741 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    22801 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    22861 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    22921 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    22981 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    23041 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    23101 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    23161 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    23221 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    23281 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    23341 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    23401 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    23461 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    23521 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    23581 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    23641 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    23701 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    23761 cacactcccg ttttatctct ttggaatgaa aaagtatcag gattttttct tgctgctatt
    23821 caaaccaaac gggattcgac tttcagtatg gtttctgata cgcttgatat tgtctttatg
    23881 ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt
    23941 agccaaaatc acaggagcta cataaggaag gacgatcgta gataaagtga tcaatactgc
    24001 actgatcata ctgctcaaac tcaccatact tgtacaaaat aaactaacga taaagatgat
    24061 acaagaataa acaaaaaata caggattaaa tcctaaaagc attcctgcac ttgtcgcaac
    24121 agcttttcct cctttgaacc cagcaaaaat cggaaatgta tggccaagaa tggcgcatac
    24181 cccaaaccaa agagggttaa tcccagaaag gtggaagatc atcggcaaac aagtagccaa
    24241 tgtacctttt aaaatatcca taagcaggac aacaatcccg gctgttttgc ctaatacacg
    24301 aaatgtattg gtcgttccag tattaccgct cccgtgttgt cggatatctt ttttgaaaaa
    24361 caatttgcca atccagacac cggaaggaat agaacctaaa agatatgcta aaataagtaa
    24421 taagatcaat ttcatttttt accctctttc aagttgaaaa ctatccttta ttttagcatg
    24481 aataggtaac aggaacaatt gaaaaaatga aagatacaag tataatagat ggaataaaaa
    24541 gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa cacaagaaac gattttcaac
    24601 tatttggaag aagcaaaaaa tattgccgtc gtaggcttga gcaataaacc agaacgaaca
    24661 agttacatgg ttgcacaggt attgcaagac aatggatata aaatcatacc agtaaatcca
    24721 ttattggctg gtgaagaaat tctaggcgag aaagtctatg cgaatttgac agaaatcgat
    24781 gatccaatcg atatcgtaga tgtatttaga cggagtgaat ttttacctga aatagcaggt
    24841 gattttttga aaactgatgc taaagtgttc tgggcacaat tagggattga aaacaaagaa
    24901 gcagctgaga tgctaagaaa agcaggacgt acagatatcg taatggatcg ttgtattaag
    24961 attgaacttg gtaagttgaa agaaaaccaa aaataaaaat gaccacaaag aagagatgat
    25021 cgatagaggg gaatgtcgaa aaagcggtta ggagataatt aaaattgatt tctcgtctac
    25081 cctctttttt ttcacgacct tttctagtat gataatcgtg gtgcggtttt gccgcaaagt
    25141 attattgaag gagcgtttgc cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc
    25201 atccaagtat tggaaggatt agaggcagtt agaaaaagac cagggatgta cattggatcc
    25261 actgacagcc gaggcttgca tcatttagtc tatgaaatcg tcgataatgc agttgacgaa
    25321 gcattatcag gttatggcag tgagattgat gtgaccatcc atgaagacaa tagtataact
    25381 gttgcagata gcggacgtgg gatgcctgta ggaatgcatg cttccggaat accaacagtt
    25441 gaagtgattt ttactgtgct gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact
    25501 tctggtggac tgcacggtgt aggagccagt gtggtaaatg ccttatcgaa atggctaatt
    25561 gtaacgatcg ttagagatgg cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct
    25621 gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt
    25681 ttaccagatg atacaatatt cagtacaacg aaattttcat atgaaatttt agccgaacgg
    25741 ttaagagaat ctgcctttct tctaaaaggc gttaaaatct ctctttctga tcttcgtgga
    25801 gaagaaccag tcaaggaaat ttttcattac gaagaaggaa tcaaagaatt tgttgattat
    25861 ctgaatgaag aaaaagatac attgacacca gttgtttatt tttcaggaga aaaagaaggt
    25921 atcgaagtag aggtcgctta tcaatacaat gacggttatt cagaaaatgt tttatctttt
    25981 gtcaataacg ttcggacaaa agatggggga acccatgaag ctggtatgaa agcggcgatg
    26041 acgaaatctt ataatgaata tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat
    26101 ctggaaggta gtgatttcag agaagggtta gctgctgtac tttctatccg tgtacctgaa
    26161 aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc
    26221 gtagtagata acgtcatcag tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg
    26281 agccagatgt tggtccgtaa agcaatcaaa gccagagaag cgagagaagc agcacgtaaa
    26341 gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag gcgaatctct tctttcagga
    26401 aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg
    26461 gattctgctg gcggatctgc taaacaagga agagacagaa agttccaagc aattctacct
    26521 ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa
    26581 gaaatcaata cgatgatcta tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa
    26641 gactgtaatt acgacaaagt aatcatcatg accgatgcgg atacagacgg cgcccatatt
    26701 caagtattac tgcttacttt cttttatcgt tatatgaaac cgctgattga agctgggaaa
    26761 gtatatatcg cgttaccgcc tttatataaa gtatccaaag gacaaggaaa gaagcaagtc
    26821 attgaatatg cgtggacaga tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga
    26881 tatatgctgc agcgatataa aggacttgga gagatgaatg cggaacaatt gtgggaaaca
    26941 acgatggatc cgacttcccg tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct
    27001 gaacgtcgag taaccacact gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa
    27061 aatcacgtgc aatttacgct cgaagaagat ggcagtatct tagataaaaa agaggatact
    27121 gaaatctctc cttcagtttc taatgattta ttagatgaag aacgagcaga taaaaatgaa
    27181 aacaatcagt tatttgaagt agaataggag gtcacctgat ggaaaatcga caagaagtac
    27241 aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc aaatatatta
    27301 tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa cgccggatat
    27361 tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat
    27421 ctgtcggaaa tatcatgggg aattatcacc ctcatggtga ccgcagtatt tatgaagcga
    27481 tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg cacgggaata
    27541 acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc agattatcca
    27601 agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc gtgtggaatt
    27661 ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac cttctcgtca
    27721 atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat aacttagctg
    27781 aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt gaaaaattga
    27841 tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga aaagatgaaa
    27901 tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa acaaagatag
    27961 aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat gaagtaaata
    28021 aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc gatggaatag
    28081 ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag
    28141 acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta cagatcaatt
    28201 acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga ctgaaagaca
    28261 ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt caattcgatt
    28321 tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct ttatcgattc
    28381 tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt
    28441 tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca ttgcaattat
    28501 accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc ttgattgcgc
    28561 aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct gttatgaaaa
    28621 aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca atcgaagacg
    28681 aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac gtaattgtca
    28741 gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt gcttccaaac
    28801 cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa ttgagtacat
    28861 tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct gtccatgaac
    28921 tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc tccaatctgt
    28981 ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca gagaaaacat
    29041 ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag ttcgaaccat
    29101 ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa gaagatgaac
    29161 tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta gtaacgcatc
    29221 aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca aaggcagctg
    29281 gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg cttgtgttag
    29341 cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg
    29401 ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg cgtgagctga
    29461 aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa ataaccttga
    29521 tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata ggtgatcgaa
    29581 catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc gaagttatcg
    29641 atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa gagacgccat
    29701 ctgtattaat acaggtggcg tctctttttc attcattttt aataaatcct tatacgtttg
    29761 caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt atgtcaacgt tgatttaaat
    29821 atatgtaaat aaaacataaa aattgttaaa taaaaaacaa gaaaaccctt gcaaagatag
    29881 ggtgctgtgt tatactaatc acgtaaaaac tgttacataa aacagaaatt taggaggtcc
    29941 atacaatgga gcaatggaat ggatttaaag gtaccaaatg gaaagaaggc gtagacacta
    30001 gagactttat tcaaaataac tacactgaat atcgcggtga tgacagcttt ttggaaggcc
    30061 caacagaagc aacagaaaaa ttatgggatg aattgcaaaa gttaaatgat atccaattcg
    30121 agcgtaacgg tgtctatgat atggacacag aaatcgtatc aactattact tcacacgatg
    30181 caggctattt ggataaagat ttggaaaaaa tcgtcggtct acagacagac aaacctttga
    30241 aacaagcatt catgccgttc ggcggaatca atatggctaa caatgcgcta aaaagcaatg
    30301 gctatacacc aaacgatgaa ttgacacata ttttcactga ctggcgcaaa acacataatc
    30361 aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc tcgtaaaaac aaaatcatta
    30421 cagggcttcc agacgcttac ggtcgcggac gtatcatcgg tgactatcgt cgtgtggcat
    30481 tgtatggaat cgactatttg atggaacaaa agaaaaaaga tcatgatatg actggttatc
    30541 atacaatgag tgaagaagtg attcgtttac gtgaagaaat cactgaacaa tatcgtgcat
    30601 tagcgcaaat gaaagaaatg gctgcgaaat atggctatga tatttctaaa ccagctgcaa
    30661 atgcaaaaga agctgtccaa tggctatact tcggatattt agcagcaatc aaatcacaaa
    30721 atggtgctgc gatgtcaatc ggacgtatct cagcattcct tgatatctat atccaacgtg
    30781 atttggataa tggagtaatc acagaaaaag aagcacaaga attgatcgac cacttagtaa
    30841 tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata taaccaatta ttctcaggat
    30901 tccctatctg ggctacttta tccattgcag gtatgggatt agatggtcgt tcattagtta
    30961 caaagaatga tttccgtctg cttcatacat taacaaatat gggaccttca ccagaaccaa
    31021 acttgactgt tttgtattct gaacaccttc cagaaggatt cagaacatat gctagcagaa
    31081 tcgctatcga aagttcttcg attcaattcg aaaatgataa tcagcttcgt gaacaatggg
    31141 gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac agttgtcgga aaagacatgc
    31201 aattctttgg cgctcgtgct aacttggcta aaacactatt atatgccatc aatggcggag
    31261 tcgatgaagt gactggcgcc caagttggac cagaatatcg tccaatcaca tcagaaatac
    31321 tagactttga agaagtgaaa cataacttca tgaatatgat ggattggtta gcagaactat
    31381 atgtcaatac gttgaacatc attcattata tgcatgataa atattcttat gaagcagctc
    31441 agctggcttt gatggattct gacttgcaac gcacatttgc tacaggtatt gctgggatct
    31501 ctcatgcggc agacagcttg tcagcgatca aatatgcaaa agtaaaagca atccgtaatg
    31561 aaaaaggaat agcagttgat tttgaagtgg atggcgactt ccctaaatac ggaaatgatg
    31621 atgaccgtgc agatgaatta gtttcatgga tcttggaata cttcatggga caaatcaaac
    31681 gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt attgacgatt acttcaaatg
    31741 ttgtttacgg taaaaataca ggaaatactc ctgatggccg tgaagctgga aaaccacttg
    31801 cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg gttattagct agcctgaact
    31861 caacagctaa aatgaattac ggctgtgcat tggatggaat ttcaaatacc caaacaatca
    31921 atccttcagg tcttggaaaa gaacaagata caagaatcga aaacttgcgt aatgtcttag
    31981 atggatactt ctcaaaagga gcttatcatt tgaatgtcaa tgtattcagt aatgatttat
    32041 tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt gacgatccgg gtatctggtt
    32101 atgcagttaa attccgtgac ttgacaagag aacaacaatt agatgtcttg atgagaactt
    32161 cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc gacttagcgt cgaaaggacg
    32221 ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct tatttgaaag aaacgattta
    32281 caggggcagg acttgtatgt tccgtccctg taaattttac acataacgaa gcggcaattg
    32341 cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac ttgtacttaa aatgaaatca
    32401 aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg ctatgttcat tctatcgaaa
    32461 ctttcggttc agtagacgga cctggactgc gctttgtcgt gtttatgcaa ggatgccgca
    32521 tgcgttgcca attttgtcac aatccagata cttggaatat tggcggaggg aaagaataca
    32581 cagcagatga acttttagat aaagcagaac gcttcagacc ttactgggga gataaaggtg
    32641 gtattactgt tagtggtgga gaacctctat tgcagatcga ttttttgatc gaactattta
    32701 aaaaagcaaa agagcggaag atgcatacta cattggatac atgcggaaaa ccttttacgt
    32761 atgaagaacc tttcttttca cgttttcaag aattgatgaa gtatacagat ttactgttat
    32821 ttgacatcaa acacatcgat aatgaagagc ataaaaaact aacgcattgg gacaatgaaa
    32881 atattttaga aatggctcaa tatttgtcaa aaatcaacaa accggtttgg attcgccatg
    32941 tactagttcc tgagcgaagt gattatgatg aatatttgat ccgactagac aactttattc
    33001 aaaccttgtc aaatgtagac agagtagaga ttcttcctta tcatacgatg ggaaaataca
    33061 agtgggaaac acttggtttg aaatatccat tagaagggat cgaaccacct acaaaagaac
    33121 gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta tacaaaatat ttaacgcgat
    33181 aggtcgcatc ggaaagaaga taagtattgc ttctttccgt ttttttgcta ttattaaata
    33241 ggagaaatca caaaggagcg aatgacatgt caaaaatttt agtttttggt caccaaaatc
    33301 cagatacaga tgcaatcggg gcagccattg cgttttcaca cttgcaaaat gaattaggta
    33361 aagaaacaga agcagttgct ctaggtacac ctaatgaaga aacacaatac gcattagacc
    33421 actttcagct atctgcacca cgagtagtag agtcagctaa agcagaaaat gcagatgtca
    33481 tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga tatcagcgaa gtaaatattt
    33541 tagctgtagt agaccaccat cgtattgcaa actttgaaac agctaatcca ttgtactatc
    33601 gtgcagaacc agttggatgt acaagtacga ttattttaaa actatataaa gaaaaccaag
    33661 taacaattcc taaagaaatc gcaggaatca tgctctcagc tatcatttca gatacattgt
    33721 tgttcaaatc accaacatgt acgcctgaag atgaagcagc tgcaaaagaa cttgcagcaa
    33781 tcgctgaagt cgatctaaac acttatggac ttgaaatgtt gaaagcagga acaaatctaa
    33841 gtgataaatc agctgcggtt cttcttgatt tggatgcaaa aagtttccca atgggcagtt
    33901 ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa tgaagtgatg gatcgacaag
    33961 cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa tggctacgat ctgttcgtat
    34021 tagttgtcac aaacatctta gacagtgatt cagaattact tgttgctggt aaaggaaaag
    34081 aaaaagtaga agaagctttc caaacaactt tgaccaacaa ccgtgctttg cttaaaggtg
    34141 tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc tgtatttagc taatcaaatg
    34201 gtagtatgaa gcattcaaaa tgaatggata agcgattgat ataaaaagaa acatagaaca
    34261 tactaaaggc cgaagaaatg gaggagaata acagtcaatt atgactcttg ttcttcctcc
    34321 tttttacgta aaagcctttt aagtaaaata aaacggatag aaaagagaac atatatgaac
    34381 tattattcaa aaaataagga aaacttttat caaaaactga ccgatgatcc gcttttttct
    34441 tcattgaccg attatctata tgaacatcga gaacaagaaa cgattctaag agaattgaaa
    34501 aaagaatttt cgcaaaataa attttcccat tttcttgatt tattgatcga tgcaggatta
    34561 atcaagaggg aagaacgaag atatcatttg aactttccaa tatttgatcc taaagattac
    34621 ttgcaacaag ccacttcggc tgctgaaacc atagctgaac aattgaagag actctctgtt
    34681 gatgaacaaa aattagcgat gggagaagtt atttgggcgt attgtttcga agatgaacgg
    34741 aaagaagcgt atttttatgg agtaagaaac agccgagaga cagaattgct gaggacaact
    34801 gcaggaaatg agaagtaccg ttttatcaca ctttcctcta tagaacactt cccgcttact
    34861 ttggctaatt acttttttgt acagaagaat cagcttcctg taacgaaggc attcaaagaa
    34921 ttagccgaac ttattggaga cgtaaatgaa tcgtactttt ttgatcaaat agaagttatc
    34981 gttgacagga tacgtaagaa taaatataaa aatcgccggc ctagtatttt ccatcagtct
    35041 ctacttgtga cgaatactat aaaggaagaa gaatcattca cattagaatt acctattgtc
    35101 gaaaaaaaca attttgagat tgaattacca acattggatc cttcattgac aatggaggaa
    35161 atggcctttc ttaaacgaca aatcttttct gagctgagca agaaatttat accgcatgca
    35221 ttttcttata tcaaagaata tggaacagtt ttagtgagta aaacctaaga gagcagtaag
    35281 caaaaattat actgactctt gcaggaggag cctttctctt cctttatggt taaaagaatt
    35341 atttgagtat tttgcctatc taccacctat tagattcact acatggtctg gaaacaaagg
    35401 aatgcaaaaa aatggtgaga aatgtggaat gacaaaggaa gtagtgataa gaaaagttag
    35461 atttttaaat aatcaatatt atgattcagt caagtatggc attttatggg aagaactagc
    35521 tgattagatt aggaagtcca tttgataatc cagaatcact aagaaacgaa tggcgagtca
    35581 ttaataattt atccaatctt gggttattct tttagattga agtgtttaaa actaaaaaag
    35641 catttatgaa gtcgtaatag tcaggcttca aaatgctttt tattttgttt gttttttctt
    35701 tgccacataa atacacctag actgacgatg aagagcatat ccaacagatt tcctgcgact
    35761 tggatcgtcg aagggttggt cagtggcatt aaagcaagga caaaaaagga aaacaagctg
    35821 taaataaaac aggtgacagg aactaatcga aagaagagcg gacatttcaa ttggctaaaa
    35881 atgagtgaaa aaataaaata agtaaaaatg gataaaatca cgactaaacc attcatgata
    35941 aagaagaaaa gcgtattgtg attaaacgaa taaaaaagaa ttgctaaaaa agaaaacgtt
    36001 ttaaatgcaa tcaatacgct tgaagcttcg tttaacgtac caggatgtcg atgaaaaaaa
    36061 taggcaccga aataaacaga aaacagtaag ccgcaaagag aagacaaaat caaaaaaaac
    36121 ggattttctt cggcttctgt gatatcagaa tgatccaaaa gcaaccagcc taatgcacta
    36181 gaaggcgcat gtgaggtctt aaggtatgaa taacatagaa aaaataagat cgtcaaagtt
    36241 gcaaaaaaag agcctaaatg taaaaaatta gtggttctac gttgaatttc cataatatct
    36301 ccaaattaaa ttgataagta tataaa
//
LOCUS       DACDEA010000026        35933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDEA010000026 DACDEA010000000
VERSION     DACDEA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.026"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IV136_001283"
     CDS             25..696
                     /locus_tag="IV136_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8465284.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IV136_001284"
     CDS             755..1195
                     /locus_tag="IV136_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465285.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IV136_001285"
     CDS             1207..1875
                     /locus_tag="IV136_001285"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8465286.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IV136_001286"
     CDS             1939..2562
                     /locus_tag="IV136_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8465287.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IV136_001287"
     CDS             2618..3004
                     /locus_tag="IV136_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8465288.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IV136_001288"
     CDS             3217..4608
                     /locus_tag="IV136_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8465289.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="IV136_001289"
     CDS             5219..5587
                     /locus_tag="IV136_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8465290.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="IV136_001290"
     CDS             5767..6708
                     /locus_tag="IV136_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8465291.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="IV136_001291"
     CDS             6777..7508
                     /locus_tag="IV136_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8465292.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="IV136_001292"
     CDS             7652..9832
                     /locus_tag="IV136_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8465293.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="IV136_001293"
     CDS             9852..10334
                     /locus_tag="IV136_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8465294.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="IV136_001294"
     CDS             10350..11111
                     /locus_tag="IV136_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8465295.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="IV136_001295"
     CDS             11242..12576
                     /locus_tag="IV136_001295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8465296.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="IV136_001296"
     CDS             12883..13368
                     /locus_tag="IV136_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8465297.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="IV136_001297"
     CDS             13361..14044
                     /locus_tag="IV136_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8465298.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="IV136_001298"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="IV136_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8465299.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="IV136_001299"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="IV136_001299"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8465300.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="IV136_001300"
     CDS             17660..18289
                     /locus_tag="IV136_001300"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8465301.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="IV136_001301"
     CDS             18341..18673
                     /locus_tag="IV136_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8465302.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="IV136_001302"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="IV136_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8465303.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="IV136_001303"
     CDS             19499..20872
                     /locus_tag="IV136_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8465304.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="IV136_001304"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="IV136_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8465305.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="IV136_001305"
     CDS             21927..22367
                     /locus_tag="IV136_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8465306.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="IV136_001306"
     CDS             22511..23212
                     /locus_tag="IV136_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8465307.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="IV136_001307"
     CDS             23209..24330
                     /locus_tag="IV136_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8465308.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="IV136_001308"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="IV136_001308"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8465309.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="IV136_001309"
     CDS             25709..26617
                     /locus_tag="IV136_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8465310.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="IV136_001310"
     CDS             26652..26924
                     /locus_tag="IV136_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8465311.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="IV136_001311"
     CDS             26935..27981
                     /locus_tag="IV136_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8465312.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="IV136_001312"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="IV136_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8465313.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31663)
                     /locus_tag="IV136_001313"
     CDS             complement(30992..31663)
                     /locus_tag="IV136_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465314.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(31656..32150)
                     /locus_tag="IV136_001314"
     CDS             complement(31656..32150)
                     /locus_tag="IV136_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8465315.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="IV136_001315"
     CDS             complement(32170..33390)
                     /locus_tag="IV136_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465316.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="IV136_001316"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="IV136_001316"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8465317.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..>35933
                     /locus_tag="IV136_001317"
     CDS             35568..>35933
                     /locus_tag="IV136_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465318.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDI"
BASE COUNT        12078 a         6300 c         7702 g         9853 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat att
//
LOCUS       DACDEA010000027        34036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDEA010000027 DACDEA010000000
VERSION     DACDEA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.027"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..413
                     /locus_tag="IV136_001318"
     CDS             <1..413
                     /locus_tag="IV136_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8465319.1"
                     /translation="TNEDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDF
                     KALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPG
                     RPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            450..1460
                     /locus_tag="IV136_001319"
     CDS             450..1460
                     /locus_tag="IV136_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8465320.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1575..2288)
                     /locus_tag="IV136_001320"
     CDS             complement(1575..2288)
                     /locus_tag="IV136_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465321.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2478..3068)
                     /locus_tag="IV136_001321"
     CDS             complement(2478..3068)
                     /locus_tag="IV136_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8465322.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3552..3779
                     /locus_tag="IV136_001322"
     CDS             3552..3779
                     /locus_tag="IV136_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8465323.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3795..4961
                     /locus_tag="IV136_001323"
     CDS             3795..4961
                     /locus_tag="IV136_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8465324.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5195..6238
                     /gene="hrcA"
                     /locus_tag="IV136_001324"
     CDS             5195..6238
                     /gene="hrcA"
                     /locus_tag="IV136_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8465325.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6332..6895
                     /gene="grpE"
                     /locus_tag="IV136_001325"
     CDS             6332..6895
                     /gene="grpE"
                     /locus_tag="IV136_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8465326.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            6946..8775
                     /gene="dnaK"
                     /locus_tag="IV136_001326"
     CDS             6946..8775
                     /gene="dnaK"
                     /locus_tag="IV136_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8465327.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8926..10092
                     /gene="dnaJ"
                     /locus_tag="IV136_001327"
     CDS             8926..10092
                     /gene="dnaJ"
                     /locus_tag="IV136_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8465328.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10174..10839)
                     /locus_tag="IV136_001328"
     CDS             complement(10174..10839)
                     /locus_tag="IV136_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8465329.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10815..10988
                     /locus_tag="IV136_001329"
     CDS             10815..10988
                     /locus_tag="IV136_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8465330.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11216..11422)
                     /locus_tag="IV136_001330"
     CDS             complement(11216..11422)
                     /locus_tag="IV136_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8465331.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(11448..11867)
                     /locus_tag="IV136_001331"
     CDS             complement(11448..11867)
                     /locus_tag="IV136_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8465332.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            12371..12724
                     /locus_tag="IV136_001332"
     CDS             12371..12724
                     /locus_tag="IV136_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465333.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(12784..12951)
                     /locus_tag="IV136_001333"
     CDS             complement(12784..12951)
                     /locus_tag="IV136_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465334.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(13070..14989)
                     /gene="tet(M)"
                     /locus_tag="IV136_001334"
     CDS             complement(13070..14989)
                     /gene="tet(M)"
                     /locus_tag="IV136_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8465335.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(15005..15121)
                     /locus_tag="IV136_001335"
     CDS             complement(15005..15121)
                     /locus_tag="IV136_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8465336.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(15366..15443)
                     /locus_tag="IV136_001336"
                     /pseudo
     CDS             complement(15366..15443)
                     /locus_tag="IV136_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(15597..16232)
                     /locus_tag="IV136_001337"
     CDS             complement(15597..16232)
                     /locus_tag="IV136_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465337.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            16744..17886
                     /locus_tag="IV136_001338"
     CDS             16744..17886
                     /locus_tag="IV136_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8465338.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17879..18085
                     /locus_tag="IV136_001339"
     CDS             17879..18085
                     /locus_tag="IV136_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8465339.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            18164..19357
                     /locus_tag="IV136_001340"
     CDS             18164..19357
                     /locus_tag="IV136_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8465340.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(19364..19522)
                     /locus_tag="IV136_001341"
                     /pseudo
     CDS             complement(19364..19522)
                     /locus_tag="IV136_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(19528..20265)
                     /locus_tag="IV136_001342"
     CDS             complement(19528..20265)
                     /locus_tag="IV136_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8465341.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20267..21769)
                     /locus_tag="IV136_001343"
     CDS             complement(20267..21769)
                     /locus_tag="IV136_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8465342.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22028..24022
                     /gene="uvrB"
                     /locus_tag="IV136_001344"
     CDS             22028..24022
                     /gene="uvrB"
                     /locus_tag="IV136_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8465343.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24032..26848
                     /gene="uvrA"
                     /locus_tag="IV136_001345"
     CDS             24032..26848
                     /gene="uvrA"
                     /locus_tag="IV136_001345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8465344.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27111..28376
                     /locus_tag="IV136_001346"
     CDS             27111..28376
                     /locus_tag="IV136_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8465345.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28380..29111
                     /locus_tag="IV136_001347"
     CDS             28380..29111
                     /locus_tag="IV136_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8465346.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29246..30130
                     /gene="rapZ"
                     /locus_tag="IV136_001348"
     CDS             29246..30130
                     /gene="rapZ"
                     /locus_tag="IV136_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8465347.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30127..31125
                     /locus_tag="IV136_001349"
     CDS             30127..31125
                     /locus_tag="IV136_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8465348.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31159..32094
                     /gene="whiA"
                     /locus_tag="IV136_001350"
     CDS             31159..32094
                     /gene="whiA"
                     /locus_tag="IV136_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8465349.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            32637..33287
                     /locus_tag="IV136_001351"
     CDS             32637..33287
                     /locus_tag="IV136_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465350.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33474..>34036
                     /locus_tag="IV136_001352"
     CDS             33474..>34036
                     /locus_tag="IV136_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8465351.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11172 a         5979 c         6954 g         9931 t
ORIGIN      
        1 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
       61 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
      121 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
      181 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
      241 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
      301 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
      361 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
      421 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
      481 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
      541 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
      601 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
      661 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
      721 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
      781 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
      841 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
      901 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
      961 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
     1021 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
     1081 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
     1141 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
     1201 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
     1261 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
     1321 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
     1381 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
     1441 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
     1501 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
     1561 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
     1621 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
     1681 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
     1741 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
     1801 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
     1861 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
     1921 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
     1981 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
     2041 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
     2101 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
     2161 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
     2221 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
     2281 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
     2341 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
     2401 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
     2461 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
     2521 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
     2581 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
     2641 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
     2701 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
     2761 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
     2821 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
     2881 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
     2941 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
     3001 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
     3061 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
     3121 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
     3181 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
     3241 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
     3301 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
     3361 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
     3421 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
     3481 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
     3541 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
     3601 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
     3661 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
     3721 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
     3781 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
     3841 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
     3901 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
     3961 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
     4021 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
     4081 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
     4141 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
     4201 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
     4261 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
     4321 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
     4381 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
     4441 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
     4501 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
     4561 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
     4621 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
     4681 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
     4741 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
     4801 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
     4861 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
     4921 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
     4981 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
     5041 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
     5101 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
     5161 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
     5221 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
     5281 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
     5341 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
     5401 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
     5461 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
     5521 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
     5581 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
     5641 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
     5701 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
     5761 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
     5821 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
     5881 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
     5941 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
     6001 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
     6061 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
     6121 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
     6181 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
     6241 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
     6301 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
     6361 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
     6421 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
     6481 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
     6541 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
     6601 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
     6661 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
     6721 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
     6781 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
     6841 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
     6901 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
     6961 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
     7021 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
     7081 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
     7141 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
     7201 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
     7261 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
     7321 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
     7381 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
     7441 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
     7501 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
     7561 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
     7621 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
     7681 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
     7741 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
     7801 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
     7861 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
     7921 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
     7981 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
     8041 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
     8101 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
     8161 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
     8221 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
     8281 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
     8341 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
     8401 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
     8461 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
     8521 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
     8581 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
     8641 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
     8701 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
     8761 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
     8821 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
     8881 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
     8941 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
     9001 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
     9061 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
     9121 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
     9181 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
     9241 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
     9301 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
     9361 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
     9421 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
     9481 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
     9541 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
     9601 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
     9661 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
     9721 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
     9781 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
     9841 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
     9901 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
     9961 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    10021 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    10081 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    10141 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    10201 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    10261 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    10321 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    10381 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    10441 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    10501 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    10561 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    10621 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    10681 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    10741 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    10801 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    10861 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    10921 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    10981 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    11041 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    11101 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    11161 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    11221 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    11281 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    11341 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    11401 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    11461 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    11521 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    11581 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    11641 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    11701 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    11761 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    11821 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    11881 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    11941 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    12001 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    12061 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    12121 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    12181 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    12241 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaaatataa acctataaac
    12301 catataacac gtttttctat acctgttttt aattcagtag gaacaataaa atgtatagag
    12361 gtggtctact atgcgtaaaa aagaagataa atatgatttt agagcctttg gtttagccat
    12421 taaagaagct cgattgaaac gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat
    12481 tgacccacgg tacttaacta atattgaaaa taaagggcaa caccccagca tacaagttct
    12541 ttatgacctt gtatcgttac ttcatgtttc cgttgatgaa tttttcttac ctgctaataa
    12601 cttggtaaaa agcacccgac gattacagat agagaaatac atggatagct ttacagacaa
    12661 agaactatcc ttaatggaat ctttagccag cggtatcaac gaagcaagaa acatcgaaga
    12721 ctaattaaaa gaatccatac ataacggaaa gagccgataa aatgagattg tattaatctc
    12781 attttatcgg ctctgcgtct ttgcgtctgg ctctgtaatc acagttactt tgaactgctt
    12841 tatttcaatt aaattttctt gtctgcattt cggacaatag agggggaatt tttttaattc
    12901 agtatcttcc cttatcttta atcgtgtttt atttccacat acaggacaca atatccactt
    12961 gtagtttata ataactatct cctcctttac actttaattc aaatctttat taaaaaatat
    13021 ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac taagttattt
    13081 tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc tggcaaacag
    13141 gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta cgtccatttg
    13201 taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt tctccactaa
    13261 gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat ttaggagcat
    13321 cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta agatatggct
    13381 ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga gcaagcatcc
    13441 gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa cagattttac
    13501 agtccgtcac attccaacca tacaatcctt gttcacagcc atagcgtatc ccctccataa
    13561 ctgcattttg aaacgattga tttaagtatc caagagaaac cgagctctca tactgcattc
    13621 cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa ggatttggcg
    13681 gcacttcgat gtgaatggta tattctgcat tttttaacgg tctctccata taaatgactg
    13741 taggctcttt tagttctatc tccacatgat acttttcttg caacagtgca ctaatcactt
    13801 ccatttgtac tttccctaag aaagaaagta taatttcatg tgtcgtagaa tccacgtaat
    13861 atcgtagaag cggatcacta tctgagattt ccaaaagggc atcaagcaac atttctctct
    13921 gttcaggttt actcggttca acagttgttt gtagtagagg gtgcggattt tcaatctttt
    13981 ttctctgtgg caatagtttt gtatctccaa gaacactatt taacttcaaa aactcatttt
    14041 gcaaaataac aatttctcca gaataagctc tatcaatctt acataattca ccatttattg
    14101 aagtatacat ttctgtaact tttatttttt ctttttctga tactctaacc gaatctcgta
    14161 aatgtagtac tccactataa aggcgtatat atgcaagacg ttgtcttttt tttgtatatt
    14221 caattttgaa aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt
    14281 tattagtaat aacttctata aggttatcaa tccctatatt actttttgca cttccatgat
    14341 aaagagggaa cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg
    14401 cttctaatga tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg
    14461 tatcccattg ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct
    14521 tctgtttgat tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata
    14581 aatcaattcc attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc
    14641 taagtgcatg aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta
    14701 gaattgcccc atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc
    14761 ctggcgtgtc tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct
    14821 gaattgtaat tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt
    14881 ccacgcttcc taattctgta atcgctccac tgttatataa taagctttct gttaaggtag
    14941 tttttcctgc atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct
    15001 ccattcaaaa acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat
    15061 ttttatgcat aaccataggc atacaaagca tacagatatt ctccggatac tttagaatca
    15121 catgataaag gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc
    15181 caattatttg ttcccactat caaattgaca gtttatttaa gaataccttg ccgcatattt
    15241 attaactcct tttaaataga tacttaaata atagcacgta agagcatatt tgtaaaggaa
    15301 tctccaattt tttatcaaag agagtacgtg attacaaaat agctgtaata atgtaccaat
    15361 atttgttatt ctataatctt ccaattactc ccgttctttt caagtaccaa atcaaattga
    15421 gatacctgcg ttgcctttgc ctgatttacc acaaatcaga ttttatccct ttcttcctct
    15481 tgattgccca caacagaaaa gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc
    15541 tttgcactct tgatcgggct ttttgtttgc tgtatcttct cgcaagagga aaaaagttac
    15601 tcgtcctcat catcaaaatt gttattcctt aatacttctc gtaacagttc catatcttct
    15661 tttgagttcg ggtttatcat tttcacgact tgataaggtg tcttatactt atgtccctct
    15721 atgctttccc caaacatatc catactgtat aaatcatcat agcggtctaa taatttttga
    15781 aacttcaaat gctggataaa ttctttgatt gggtaaaggt cggacgcttc aatgcttttc
    15841 ccgttgatat agtctgtaat atattcccgt aacttttcta actcatattc cagccattct
    15901 tcctcgctgt caaagaagtt atcatagtgt ccatgtttca gttctgcatt tagacgttct
    15961 tcggctctta aaaactgaca gacttccttt tcagattgat tgacatactt ccattctccc
    16021 gacaacaatt cattgggcgt aacgtccagc acttccatta tcttttccag cgtttcatag
    16081 gcaggatagt tcaaccctct ttcaatcttg gaaaggctct gcatattgat accgattttg
    16141 tccgcaagtt cctgttgttt cattccccta aattttctta tggtctgtat attatttcct
    16201 aaatggctta ttttcattgc ataaccctcc atatctcgtt atatcttcgc tattataagt
    16261 ctatcatgct taatatgatt tgtaaaggca aaaaaagata ttgacaaata agcatataag
    16321 aattactatg ttcttgtaat cgaataagca caatagaatg aaatataaaa acattcaaaa
    16381 agaaatacct atctgattaa atccgtagat atgaatatcg gttgaaatgg aacttgttag
    16441 aaaaggcaaa acagaacttg ctgacaaagt atttctgaca cggtggcgga agcctctcag
    16501 gcttaccgcc atgacacctt gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg
    16561 cgagccttgt ctgtgccccc gttatctaac aggggggcac tatttcacaa aagacaagtc
    16621 aaaaaccata caatcccaaa gttgtgagta gtaatgagaa gttttaagaa gatagtgcat
    16681 atcacacttt ttgaatagag gtttacaggt atttcaaggc tattgtggga ttggaggaat
    16741 acaatgaaca atttagaatt tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt
    16801 tcacaaggag aacttgcaag tcttacccat gtatcaagat atagtatcaa ccgctttgag
    16861 aacggaaaag ccaacgccag caaggaaaca caaaatatta tcctgcgttg tctgaattat
    16921 tatatctgcg ataagccttt ttatctgctc gttgattatc tgtctgtccg ttatccaacg
    16981 actgacgcat tggaaattat ccgaaaggtg cttggcatga aagcagatta ttttatccat
    17041 tatgactatg ggtattatgg ctataaagag cattacgcct acggagaaat caaggtcatg
    17101 gcttctgatg acgaacacat gggcgtattc ttggaattaa aaggggcagg ctcacgcaac
    17161 atggaatatg tcttgcaggc acagaacaga gattggtatt catttttaaa ccgctgtctt
    17221 gactgtgacg gtgttatccg acgttttgac ttggcaatca atgatatgtg tggtctgttg
    17281 gatattcccg ttttgtctga aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat
    17341 tatgaaaatg tgcagggtgg aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt
    17401 tatatcggtt caaaggcaag cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag
    17461 gcaaccaaaa agaaacatac ggatattatc aatcgttttg aaattcgtct gcgtggtaca
    17521 aaagcagtac aggcagttga ggaattgtta ttgacatata accctcatgg gctggtgttt
    17581 taccttatta ctgattttgt gcaatttccc gactatccgc tgtgggagat attcatttcc
    17641 catgacagtt taccttttga aatgaacccc gtacctgtca atatggaacg cactctgcaa
    17701 tggctggaaa gacaggtcat gccgtcaatc gtgatgatag aggaaatcga caggctgaca
    17761 ggctcaaact acatgaaaat gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg
    17821 cttgtggaac agatgtgtac ggacatagca gacgtaatcg aaagtgaggg ggtgttttat
    17881 gagtaatgca caggatattc ccgtatggga aaaatacacc cttaccattg aagaagcgtc
    17941 aaagtatttc cgtatcggag aaaacaagtt aagacgattg gcagaggaaa acaaggacgc
    18001 tggctggctc attatgaatg gcaaccgcat acagattaaa cgccgacagt ttgaacaggt
    18061 cattgataaa ttggacgcta tctaatgcaa atgagccttg tatgtgttat gatgaacaca
    18121 agtcatatca aggctctttc caacaaggaa aggagcagac accatgaaag aaaaaagacg
    18181 ggataacaaa ggtcgtatcc tgcatactgg agagagccaa cgaacagaca gaaaatactt
    18241 atataaatat gtagacgcat ttggaaacac aaaatatgtg tatgcttgga gattgacacc
    18301 cacagacccg acaccaaagg gaaaacggga aaaaccctca cttcgagaac tggaacagca
    18361 gataagacgg gatattgagg acggtatcga cagcactggc aagaaaatga cgctttgcca
    18421 actttatgcc aaacagaacg cacagagggc aaatgtgaag aaaagcacac agaaacaacg
    18481 ggaacaactc atgcggttat tgaaagagga caagttaggt gctaggagca ttgatacaat
    18541 caaaccctct gacgctaagg aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa
    18601 taccattaac aaccataaac gctcgttaaa agcgtcattc tacatagcca ttcaagatga
    18661 ttatgtgagg aaaaatccct ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa
    18721 aaagaaagtc gcattgacag aggaacagga acaagcctta ctctcattca tcaagacgga
    18781 caatgtgtat cacaagcatt atgatgatgt gctgatactg ttaaagacag gacttcgtat
    18841 ttcggaactg tgcggactga cagtagccga cattgatttc aagaatgaag ttgtgattat
    18901 cgaccaccag ttactaaaga gcaaggaaca gggctattat attgaaacgc ctaaaacaaa
    18961 aagcggaata aggcaagtac cattaagcaa agaaacgata caggcatttc aacaggttat
    19021 gaaaaaacgt ccaaaggcag aaccatttgt gatagacgga cggggcaact tcctatttgt
    19081 caatcataag ggcaagccca aagttgcgat tgattataac gccttattta tccgtatggt
    19141 aaagaaatac aacaagcacc acaaggacaa tccattgcca catatcacac cgcatacgct
    19201 acgccataca ttctgcacaa gactggcaag caagaacatg aacccaaaag atttacagta
    19261 tatcatgggg cattcaaata tcagtatcac aatgaactgg tatgctcatg cgtccataga
    19321 taccgcaaaa tcagaggttc agcgtctaat tgcatagaag tatttaccac gattttaacc
    19381 acgcttgata gtgaaaatat aagaagatag acctagatat gtgaggttta ccacaaaagc
    19441 aaaatgcccg tagagcctat aaaataaggc tttgcggaca tttaagaaga tataaaaaga
    19501 tagtcaaaaa gacatatata atttatttta aatatttaat actttattca agaaatcttt
    19561 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt
    19621 tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt
    19681 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
    19741 gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat
    19801 cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata
    19861 catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc
    19921 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
    19981 aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt
    20041 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
    20101 ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg aagggccaat
    20161 gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc
    20221 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
    20281 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
    20341 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
    20401 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
    20461 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
    20521 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
    20581 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
    20641 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
    20701 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
    20761 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
    20821 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
    20881 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
    20941 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
    21001 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
    21061 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
    21121 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
    21181 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    21241 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    21301 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    21361 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    21421 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    21481 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    21541 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    21601 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    21661 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa
    21721 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    21781 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    21841 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat
    21901 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    21961 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    22021 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    22081 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    22141 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    22201 tgagaaagtg aataaaccaa cactgattat tgcccataac aaaacattgg ctggtcagct
    22261 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    22321 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    22381 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    22441 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga
    22501 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat
    22561 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    22621 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    22681 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    22741 agttttagga gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga
    22801 tgaacatatg gagcatgccg tagcgaatat caaagaggaa ttagaacagc gtttaactgt
    22861 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga
    22921 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    22981 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    23041 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg
    23101 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    23161 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    23221 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    23281 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    23341 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    23401 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    23461 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    23521 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    23581 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    23641 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    23701 agacaaagta acagattcta tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat
    23761 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    23821 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    23881 gtacgaagaa atgacgaagg aagaaaaaga cacgttattg atgaaattag aaaaagaaat
    23941 gaaagatgcg gcaaaagctt tggattttga aacagccgcg aatgtgcgag atatgatttt
    24001 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcatgg
    24061 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    24121 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    24181 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    24241 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    24301 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    24361 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    24421 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat
    24481 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    24541 aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga
    24601 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    24661 tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta gaggcggccc tgcgattagc
    24721 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    24781 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    24841 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    24901 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    24961 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    25021 cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg
    25081 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    25141 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    25201 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    25261 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    25321 aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    25381 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    25441 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    25501 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    25561 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    25621 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    25681 gtgggcagca gattatttga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    25741 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    25801 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    25861 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    25921 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    25981 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    26041 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    26101 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    26161 gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    26221 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    26281 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggtaaac gatataattc
    26341 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    26401 agaggatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    26461 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    26521 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    26581 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    26641 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    26701 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    26761 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    26821 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    26881 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    26941 agttctttct acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa
    27001 aagaattgag caatcggagg actttccttt gattgctttt ttctttatgg tatgatattc
    27061 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    27121 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    27181 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    27241 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    27301 accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    27361 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    27421 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaaggctga
    27481 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    27541 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    27601 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    27661 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta ggccggatat
    27721 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    27781 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgccttg
    27841 gtcatccact attagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    27901 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    27961 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    28021 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    28081 ctcgttttgt cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa
    28141 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    28201 atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    28261 gtactactgt attttatgaa aaagaccgat caatcaagcg ctggttgttg atgaaatata
    28321 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    28381 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    28441 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    28501 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    28561 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcgacca aacttcattg
    28621 tgttgacttg taacaccgca cactactttt ttgaagagtt acaagcggca acggatattc
    28681 ctatcttgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    28741 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    28801 aaaatcttgg tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa atcaattacc
    28861 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctctat tacgaaatct
    28921 tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc acagaattat
    28981 ctttgatgca tgaatttgct gaagataatc attacccagt gatcgatgct caatctattt
    29041 tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg gatacagcat
    29101 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    29161 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg gatgatataa ttttattatg
    29221 cacaatgtga gaggggttcg taaatatgac agataatttg caattagtca tcataacagg
    29281 tatgagtggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    29341 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattgatca aagaatcagg
    29401 taaagtcaca aaaatggctt taggagtgga tttgcgttct cgttccttct ttgaagaaat
    29461 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    29521 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    29581 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    29641 gaaagcccaa gcttccgtga tcattgatac gacaacattg acaccacgac aattacgtga
    29701 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    29761 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    29821 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    29881 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    29941 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    30001 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    30061 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    30121 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    30181 ggcactggtc tgccagttat cctgaaaagt ttacgaaatc aaggagcaga tatcacagca
    30241 gtagtgactg ttgcagatga tggtggaagc agcggtgcaa tcagagaatc gatcgctatg
    30301 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    30361 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    30421 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    30481 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    30541 accttgcatg ctgtttttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    30601 aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    30661 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    30721 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    30781 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    30841 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    30901 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    30961 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    31021 gtgatttcca ccgacttttt agaattgaaa gatggtggtg tcttccacga tggagacaaa
    31081 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    31141 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    31201 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    31261 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    31321 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    31381 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    31441 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    31501 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    31561 ggttcagtca ataatccgga aacgagtcgt tatcatttgg aaatattttc tatttatgaa
    31621 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    31681 gaacggcgca atggctatat ttcgtattta aaaggagcag aaaaaattgc tgatttctta
    31741 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    31801 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    31861 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    31921 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    31981 ttgaaagaat taggggaaat gattccctca ggtgcaatat caaaatcggg aattaatcac
    32041 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    32101 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagctatac
    32161 ttgcttttct tccgtcaaac tataattcca actttactgg agtaacataa tgttgaaagg
    32221 cttctattgt tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt
    32281 agttttatac ataaactttt gcttaatata gaagtcctga tatagatagg atcaagtatt
    32341 tatctataac tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact
    32401 gtttttctta cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc
    32461 attggattag ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga
    32521 tggagtgatt tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg
    32581 gtatacgtta tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt
    32641 ttataaaggg agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag
    32701 tactttttat ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt
    32761 tacttcgagc aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta
    32821 ttgtatttat ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc
    32881 cgtttagtct tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg
    32941 cagaatttat aggaagtttt agagggaatg tgacaactca acctagacga agttggagta
    33001 taaaactttt aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag
    33061 aaagtgaaga tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg
    33121 gtggaagcaa gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc
    33181 actatgactt agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt
    33241 gggaatatgc gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac
    33301 agaatgtaaa atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat
    33361 agcattgttg aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt
    33421 aacataattg gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa
    33481 cagtgagatt atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg
    33541 aatcaaaatc aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc
    33601 tttttccgat ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag
    33661 aagaaataaa tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt
    33721 ctaatgttgc ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta
    33781 tgctagagag tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa
    33841 gtatagctgt tcttttgtgc ataaagggtt atgttagcaa gaagaataaa gatattttaa
    33901 cactaatcgg tgtaaacaat ttatctagta ctaaaattat actgtttcct gaatctataa
    33961 aagaaggatc aaggttgttg ataattttta taatgcttat actgttttct gttttatttg
    34021 gggtgatgtt tataga
//
LOCUS       DACDEA010000028        33431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDEA010000028 DACDEA010000000
VERSION     DACDEA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.028"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..562
                     /locus_tag="IV136_001353"
     CDS             14..562
                     /locus_tag="IV136_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8465352.1"
                     /translation="MITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTK
                     GRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHAD
                     YLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYV
                     NTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(650..1042)
                     /gene="rpsI"
                     /locus_tag="IV136_001354"
     CDS             complement(650..1042)
                     /gene="rpsI"
                     /locus_tag="IV136_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8465353.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1058..1501)
                     /gene="rplM"
                     /locus_tag="IV136_001355"
     CDS             complement(1058..1501)
                     /gene="rplM"
                     /locus_tag="IV136_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8465354.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2020..2487)
                     /locus_tag="IV136_001356"
     CDS             complement(2020..2487)
                     /locus_tag="IV136_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8465355.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2607..4145)
                     /locus_tag="IV136_001357"
     CDS             complement(2607..4145)
                     /locus_tag="IV136_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8465356.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4225..4911
                     /locus_tag="IV136_001358"
     CDS             4225..4911
                     /locus_tag="IV136_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8465357.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5112..5639)
                     /gene="msrA"
                     /locus_tag="IV136_001359"
     CDS             complement(5112..5639)
                     /gene="msrA"
                     /locus_tag="IV136_001359"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8465358.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5723..6400)
                     /gene="pgmB"
                     /locus_tag="IV136_001360"
     CDS             complement(5723..6400)
                     /gene="pgmB"
                     /locus_tag="IV136_001360"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8465359.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6397..7527)
                     /locus_tag="IV136_001361"
     CDS             complement(6397..7527)
                     /locus_tag="IV136_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8465360.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7527..9710)
                     /locus_tag="IV136_001362"
     CDS             complement(7527..9710)
                     /locus_tag="IV136_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8465361.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9707..10732)
                     /locus_tag="IV136_001363"
     CDS             complement(9707..10732)
                     /locus_tag="IV136_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8465362.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10777..11604)
                     /locus_tag="IV136_001364"
     CDS             complement(10777..11604)
                     /locus_tag="IV136_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8465363.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11601..12668)
                     /locus_tag="IV136_001365"
     CDS             complement(11601..12668)
                     /locus_tag="IV136_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8465364.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12694..13530)
                     /locus_tag="IV136_001366"
     CDS             complement(12694..13530)
                     /locus_tag="IV136_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465365.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13527..14396)
                     /locus_tag="IV136_001367"
     CDS             complement(13527..14396)
                     /locus_tag="IV136_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465366.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14466..15749)
                     /locus_tag="IV136_001368"
     CDS             complement(14466..15749)
                     /locus_tag="IV136_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8465367.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15763..17448)
                     /locus_tag="IV136_001369"
     CDS             complement(15763..17448)
                     /locus_tag="IV136_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8465368.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17723..18748
                     /locus_tag="IV136_001370"
     CDS             17723..18748
                     /locus_tag="IV136_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8465369.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19343..22996)
                     /gene="rpoC"
                     /locus_tag="IV136_001371"
     CDS             complement(19343..22996)
                     /gene="rpoC"
                     /locus_tag="IV136_001371"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8465370.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23075..26692)
                     /gene="rpoB"
                     /locus_tag="IV136_001372"
     CDS             complement(23075..26692)
                     /gene="rpoB"
                     /locus_tag="IV136_001372"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8465371.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27420..28388
                     /locus_tag="IV136_001373"
     CDS             27420..28388
                     /locus_tag="IV136_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465372.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28406..29311
                     /locus_tag="IV136_001374"
     CDS             28406..29311
                     /locus_tag="IV136_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465373.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29308..30114
                     /locus_tag="IV136_001375"
     CDS             29308..30114
                     /locus_tag="IV136_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8465374.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30271..31239
                     /locus_tag="IV136_001376"
     CDS             30271..31239
                     /locus_tag="IV136_001376"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8465375.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31556..32080)
                     /locus_tag="IV136_001377"
     CDS             complement(31556..32080)
                     /locus_tag="IV136_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8465376.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32367..>33431
                     /gene="scm"
                     /locus_tag="IV136_001378"
     CDS             32367..>33431
                     /gene="scm"
                     /locus_tag="IV136_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8465377.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9426 a         7163 c         6289 g        10553 t
ORIGIN      
        1 ttacacactt tttatgatta ccctgtatct tggtgataga aaaagtgaga cttacgcttt
       61 gcaatggaaa tatattgatt ttgaaaaaca aacggtccgt cttaaacata ctttagataa
      121 atatcaaaga aaaaagttta caaaaggtcg aaaagatacc gtcattcaag taccagaagt
      181 tgtaatgaca ttgctaagtg aatggaaatc ggtacaagct gatcaattgt tgaaattaaa
      241 aattaagcaa acgttagatc agtatctctt tacttatact aaaccatctg gagaagtaaa
      301 ttgtcctgtt catgccgatt acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc
      361 tgacttagtt catttatctc cacacaaact ccgtcatact tatgcaacta tagctagaca
      421 aggtggagca gatatgaatc aaatttcaaa tgctttaaca cactcagata tttctacaac
      481 aaagatatat gttaacacac cagatatagt tgataaggct gtttttgaag cttttcagag
      541 aggattgaat aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc
      601 actttccaaa taattgaaaa gtgccagaaa cgttgatata taaaggatat taacgttttg
      661 agaattgtga agctttacgt gctttcttaa gacctggttt tttacgttcg accatacgtg
      721 cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg gaagtcagga tcaacttcta
      781 gcaatgcacg agcgatacca tgacggatag ctcctgattg tccagcgtat cctccgccgt
      841 ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga
      901 ttacttcacg caagtcagca tgtggaatgt attcttcaac atctttttta tttacagtaa
      961 ttttgccagt tcctggtact agacgtacgc gagcaactgc gtttttacga cggcctgtgc
     1021 cgatatattg aacttgtgcc aatgaaattc cctcctatta gattaagttt gtgatgtcta
     1081 aaacttctgg ttgttgtgct gcatgtggat gttctgctcc accgtatacg ttcaattttg
     1141 taaattgttt gcggcctaaa gtgtttttag gaagcatacc ttttacagaa gtttcgatca
     1201 aacgacgaga atttttagca cgcaattcgc cagcagtgat tgatttcaat cctcctgggt
     1261 agttgctgtg acggtaataa attttgtcag tcgctttttt acctgttaat ttcactttgt
     1321 ctgcattgat gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa gttggtttat
     1381 ttttaccacg tagtacagat gcaacaactg ttgaaagacg tcccaatgga acatcagttg
     1441 cgtctactac ataccattta cgttctactt cgcctggttt ggccatatat gttgtacgca
     1501 cgttattttc ctccaattta agttctgatg tttgacatac acttcgagtt tccggggctc
     1561 tttgtggggc aaacaatacc atttaacata ttacccgcta atctagtcaa tgtcaacgtt
     1621 tttctgcttg attgaaaaat ttatatgaaa ctttacttct tttctttctt ttatataaaa
     1681 gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac
     1741 cccttatttt gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     1801 ataaaacata agaagctgtt ttttaaaatt gtttcttgtg actcggagct gaacggcagg
     1861 cttacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
     1921 ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga
     1981 tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga tctctggcgc
     2041 tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt tgaattcgat
     2101 aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc ctttttggta
     2161 aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg gaatagtacg
     2221 atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac gcaatgtaga
     2281 ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact gagcgttcaa
     2341 atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc ctctcatgta
     2401 ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta attgatttaa
     2461 tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc ttacacttaa
     2521 aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga acgattttca
     2581 gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc ttctcgcagt
     2641 tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag
     2701 attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt caattctatc
     2761 aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc taaaatcaca
     2821 gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc cagtacatca
     2881 gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg gatctctgtg
     2941 agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca gatcccttct
     3001 agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat aaaactgcca
     3061 aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg aaaaatcaga
     3121 ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata tggattttct
     3181 cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag
     3241 atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa
     3301 caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg
     3361 ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa
     3421 agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct
     3481 gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc
     3541 tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca
     3601 aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa
     3661 aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg
     3721 ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc
     3781 cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg
     3841 ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg
     3901 ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta
     3961 attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc
     4021 atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct
     4081 tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg
     4141 ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct
     4201 tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga
     4261 tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga
     4321 tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg
     4381 atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg
     4441 ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga
     4501 aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt
     4561 tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg gattttttgg
     4621 ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg
     4681 tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg
     4741 agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga
     4801 atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc
     4861 attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt
     4921 gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac
     4981 ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct
     5041 cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct
     5101 ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa
     5161 ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct
     5221 tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc
     5281 ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta
     5341 tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc
     5401 ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca
     5461 tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt
     5521 ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct
     5581 tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata
     5641 taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt
     5701 gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg
     5761 aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta
     5821 tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt
     5881 ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga
     5941 gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa
     6001 aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca
     6061 atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa
     6121 cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa
     6181 aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct
     6241 ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa
     6301 tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa
     6361 gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg
     6421 aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg
     6481 ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc
     6541 aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac
     6601 gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc
     6661 tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag
     6721 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
     6781 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
     6841 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
     6901 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
     6961 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
     7021 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
     7081 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
     7141 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
     7201 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
     7261 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
     7321 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
     7381 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
     7441 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
     7501 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
     7561 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
     7621 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
     7681 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
     7741 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
     7801 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
     7861 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
     7921 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
     7981 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
     8041 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
     8101 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
     8161 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
     8221 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
     8281 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
     8341 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct
     8401 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
     8461 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
     8521 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
     8581 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
     8641 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
     8701 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
     8761 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
     8821 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
     8881 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
     8941 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
     9001 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
     9061 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
     9121 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
     9181 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
     9241 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
     9301 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
     9361 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
     9421 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
     9481 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
     9541 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
     9601 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
     9661 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
     9721 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
     9781 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
     9841 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
     9901 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
     9961 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
    10021 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
    10081 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
    10141 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
    10201 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
    10261 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
    10321 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
    10381 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
    10441 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
    10501 gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
    10561 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
    10621 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
    10681 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
    10741 ccctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
    10801 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
    10861 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
    10921 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
    10981 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
    11041 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
    11101 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
    11161 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
    11221 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
    11281 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct
    11341 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
    11401 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
    11461 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
    11521 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
    11581 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
    11641 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
    11701 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
    11761 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
    11821 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
    11881 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa
    11941 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
    12001 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
    12061 gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
    12121 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
    12181 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
    12241 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
    12301 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
    12361 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
    12421 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
    12481 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
    12541 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
    12601 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
    12661 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
    12721 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
    12781 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
    12841 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
    12901 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
    12961 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
    13021 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
    13081 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
    13141 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    13201 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    13261 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    13321 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    13381 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    13441 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    13501 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    13561 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    13621 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    13681 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    13741 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    13801 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    13861 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    13921 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    13981 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    14041 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    14101 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    14161 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    14221 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    14281 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    14341 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    14401 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    14461 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    14521 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    14581 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    14641 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    14701 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    14761 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    14821 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    14881 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    14941 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    15001 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    15061 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    15121 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    15181 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    15241 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    15301 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    15361 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    15421 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    15481 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    15541 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    15601 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    15661 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    15721 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    15781 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    15841 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    15901 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    15961 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    16021 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    16081 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    16141 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    16201 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    16261 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    16321 ggtcgttcca tctgggtttt tcttgtagtt tactcgggta ccctctttct tcagctgttc
    16381 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    16441 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    16501 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    16561 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    16621 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    16681 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    16741 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    16801 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    16861 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    16921 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    16981 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    17041 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    17101 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    17161 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    17221 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    17281 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    17341 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    17401 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    17461 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    17521 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    17581 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    17641 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    17701 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    17761 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    17821 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    17881 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    17941 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    18001 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    18061 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    18121 tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt
    18181 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    18241 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    18301 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    18361 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    18421 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    18481 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    18541 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    18601 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    18661 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    18721 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    18781 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    18841 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    18901 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    18961 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    19021 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    19081 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    19141 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    19201 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    19261 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    19321 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    19381 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    19441 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    19501 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    19561 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    19621 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    19681 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    19741 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    19801 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    19861 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    19921 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    19981 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    20041 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    20101 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    20161 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    20221 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    20281 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    20341 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    20401 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    20461 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    20521 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    20581 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    20641 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    20701 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    20761 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    20821 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    20881 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    20941 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    21001 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    21061 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
    21121 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    21181 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    21241 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    21301 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    21361 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    21421 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    21481 cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta acaactggtt
    21541 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    21601 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    21661 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    21721 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    21781 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    21841 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    21901 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    21961 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    22021 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    22081 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    22141 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    22201 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    22261 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    22321 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    22381 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    22441 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    22501 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    22561 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    22621 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    22681 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    22741 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    22801 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    22861 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    22921 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    22981 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    23041 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    23101 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    23161 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    23221 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    23281 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    23341 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    23401 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    23461 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    23521 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    23581 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    23641 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    23701 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    23761 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    23821 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    23881 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    23941 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    24001 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    24061 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    24121 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    24181 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    24241 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    24301 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    24361 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    24421 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    24481 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    24541 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    24601 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
    24661 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    24721 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    24781 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    24841 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    24901 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    24961 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    25021 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    25081 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    25141 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    25201 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
    25261 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    25321 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    25381 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    25441 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    25501 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    25561 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    25621 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    25681 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    25741 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    25801 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    25861 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    25921 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    25981 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    26041 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    26101 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    26161 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    26221 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    26281 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    26341 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    26401 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    26461 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    26521 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    26581 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    26641 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    26701 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    26761 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    26821 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    26881 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    26941 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    27001 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    27061 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    27121 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    27181 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    27241 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    27301 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    27361 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    27421 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    27481 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    27541 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    27601 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    27661 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    27721 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    27781 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    27841 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    27901 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    27961 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    28021 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    28081 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    28141 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    28201 tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc atggcagcaa
    28261 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    28321 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    28381 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    28441 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    28501 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    28561 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
    28621 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    28681 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    28741 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    28801 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    28861 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    28921 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    28981 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
    29041 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    29101 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    29161 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    29221 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    29281 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    29341 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    29401 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    29461 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    29521 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    29581 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    29641 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    29701 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    29761 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    29821 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    29881 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    29941 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    30001 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    30061 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    30121 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    30181 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    30241 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    30301 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    30361 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    30421 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    30481 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    30541 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    30601 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    30661 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    30721 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    30781 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    30841 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    30901 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    30961 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    31021 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    31081 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    31141 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    31201 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    31261 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    31321 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    31381 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    31441 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    31501 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    31561 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    31621 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    31681 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    31741 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    31801 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    31861 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    31921 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    31981 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    32041 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    32101 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    32161 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    32221 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    32281 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    32341 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    32401 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    32461 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    32521 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    32581 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    32641 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    32701 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    32761 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    32821 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    32881 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    32941 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    33001 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    33061 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    33121 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    33181 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    33241 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    33301 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    33361 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    33421 cagattcatc a
//
LOCUS       DACDEA010000029        31557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDEA010000029 DACDEA010000000
VERSION     DACDEA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.029"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(869..1210)
                     /locus_tag="IV136_001379"
     CDS             complement(869..1210)
                     /locus_tag="IV136_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8465378.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1210..1440)
                     /locus_tag="IV136_001380"
     CDS             complement(1210..1440)
                     /locus_tag="IV136_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8465379.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1727..2065
                     /locus_tag="IV136_001381"
     CDS             1727..2065
                     /locus_tag="IV136_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465380.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2270..2509)
                     /locus_tag="IV136_001382"
     CDS             complement(2270..2509)
                     /locus_tag="IV136_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8465381.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2485..2832)
                     /locus_tag="IV136_001383"
     CDS             complement(2485..2832)
                     /locus_tag="IV136_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8465382.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3564..4145)
                     /locus_tag="IV136_001384"
     CDS             complement(3564..4145)
                     /locus_tag="IV136_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8465383.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4328..4954)
                     /locus_tag="IV136_001385"
     CDS             complement(4328..4954)
                     /locus_tag="IV136_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8465384.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4976..6307)
                     /locus_tag="IV136_001386"
     CDS             complement(4976..6307)
                     /locus_tag="IV136_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8465385.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6462..6863)
                     /locus_tag="IV136_001387"
     CDS             complement(6462..6863)
                     /locus_tag="IV136_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8465386.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6949..7155)
                     /locus_tag="IV136_001388"
     CDS             complement(6949..7155)
                     /locus_tag="IV136_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465387.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7250..8752)
                     /locus_tag="IV136_001389"
     CDS             complement(7250..8752)
                     /locus_tag="IV136_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8465388.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8770..9225)
                     /gene="argR"
                     /locus_tag="IV136_001390"
     CDS             complement(8770..9225)
                     /gene="argR"
                     /locus_tag="IV136_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8465389.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9323..9940)
                     /locus_tag="IV136_001391"
     CDS             complement(9323..9940)
                     /locus_tag="IV136_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465390.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10161..11534
                     /locus_tag="IV136_001392"
     CDS             10161..11534
                     /locus_tag="IV136_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8465391.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11609..12721
                     /locus_tag="IV136_001393"
     CDS             11609..12721
                     /locus_tag="IV136_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8465392.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12793..14430)
                     /locus_tag="IV136_001394"
     CDS             complement(12793..14430)
                     /locus_tag="IV136_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8465393.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14598..15206)
                     /locus_tag="IV136_001395"
     CDS             complement(14598..15206)
                     /locus_tag="IV136_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8465394.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15590..16741)
                     /gene="queG"
                     /locus_tag="IV136_001396"
     CDS             complement(15590..16741)
                     /gene="queG"
                     /locus_tag="IV136_001396"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8465395.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16760..17512)
                     /locus_tag="IV136_001397"
     CDS             complement(16760..17512)
                     /locus_tag="IV136_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465396.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17653..18603)
                     /locus_tag="IV136_001398"
     CDS             complement(17653..18603)
                     /locus_tag="IV136_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8465397.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18604..19443)
                     /locus_tag="IV136_001399"
     CDS             complement(18604..19443)
                     /locus_tag="IV136_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8465398.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19440..20186)
                     /locus_tag="IV136_001400"
     CDS             complement(19440..20186)
                     /locus_tag="IV136_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8465399.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20424..21794
                     /locus_tag="IV136_001401"
     CDS             20424..21794
                     /locus_tag="IV136_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8465400.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21849..22745)
                     /locus_tag="IV136_001402"
     CDS             complement(21849..22745)
                     /locus_tag="IV136_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8465401.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23026..23703)
                     /locus_tag="IV136_001403"
     CDS             complement(23026..23703)
                     /locus_tag="IV136_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8465402.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23794..24699)
                     /locus_tag="IV136_001404"
     CDS             complement(23794..24699)
                     /locus_tag="IV136_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8465403.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24874..26328
                     /locus_tag="IV136_001405"
     CDS             24874..26328
                     /locus_tag="IV136_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8465404.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26344..27375
                     /locus_tag="IV136_001406"
     CDS             26344..27375
                     /locus_tag="IV136_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8465405.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27382..28569
                     /locus_tag="IV136_001407"
     CDS             27382..28569
                     /locus_tag="IV136_001407"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8465406.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28725..30107
                     /locus_tag="IV136_001408"
     CDS             28725..30107
                     /locus_tag="IV136_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8465407.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30234..30464
                     /locus_tag="IV136_001409"
     CDS             30234..30464
                     /locus_tag="IV136_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465408.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30664..31332)
                     /locus_tag="IV136_001410"
     CDS             complement(30664..31332)
                     /locus_tag="IV136_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8465409.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8915 a         6394 c         5695 g        10553 t
ORIGIN      
        1 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
       61 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaacttac attatactgg
      121 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
      181 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
      241 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
      301 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
      361 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
      421 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
      481 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
      541 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
      601 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
      661 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
      721 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
      781 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
      841 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
      901 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
      961 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     1021 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     1081 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     1141 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     1201 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     1261 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     1321 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     1381 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     1441 tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt
     1501 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
     1561 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
     1621 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
     1681 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
     1741 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
     1801 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
     1861 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
     1921 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
     1981 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
     2041 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
     2101 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
     2161 gatgcagtat atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc
     2221 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
     2281 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
     2341 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
     2401 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
     2461 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     2521 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     2581 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     2641 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     2701 cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg
     2761 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     2821 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     2881 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     2941 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     3001 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
     3061 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     3121 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
     3181 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     3241 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
     3301 tttcacaacc tctccactaa acgatgttca atcagctaac cttcggtatt agctcaaaaa
     3361 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
     3421 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
     3481 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
     3541 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
     3601 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
     3661 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
     3721 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
     3781 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
     3841 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
     3901 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
     3961 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
     4021 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
     4081 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
     4141 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
     4201 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
     4261 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
     4321 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
     4381 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
     4441 tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca
     4501 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
     4561 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
     4621 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
     4681 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
     4741 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
     4801 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
     4861 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
     4921 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
     4981 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
     5041 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
     5101 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
     5161 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
     5221 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
     5281 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
     5341 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
     5401 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
     5461 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
     5521 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
     5581 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
     5641 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
     5701 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
     5761 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
     5821 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
     5881 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
     5941 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
     6001 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
     6061 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
     6121 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
     6181 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
     6241 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
     6301 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
     6361 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
     6421 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
     6481 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
     6541 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
     6601 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
     6661 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
     6721 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
     6781 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
     6841 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
     6901 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
     6961 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
     7021 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
     7081 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
     7141 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
     7201 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
     7261 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
     7321 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
     7381 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
     7441 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
     7501 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
     7561 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
     7621 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
     7681 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
     7741 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
     7801 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
     7861 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
     7921 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
     7981 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
     8041 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
     8101 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
     8161 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
     8221 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
     8281 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
     8341 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
     8401 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
     8461 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
     8521 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
     8581 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
     8641 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
     8701 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
     8761 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
     8821 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
     8881 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
     8941 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
     9001 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
     9061 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
     9121 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
     9181 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
     9241 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
     9301 ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca
     9361 ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag
     9421 ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt
     9481 cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata
     9541 ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta
     9601 tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt
     9661 tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga
     9721 cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat
     9781 agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga
     9841 tccctctgat gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca
     9901 tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt
     9961 ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact
    10021 atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg
    10081 cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa
    10141 attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa
    10201 ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt
    10261 tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga
    10321 tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta
    10381 gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt
    10441 tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag
    10501 gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag
    10561 gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt
    10621 atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg
    10681 ttttgtttct ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa
    10741 gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa
    10801 catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg
    10861 gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta
    10921 ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat
    10981 tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt
    11041 ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt
    11101 ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt
    11161 ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga
    11221 ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca
    11281 ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct
    11341 tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc
    11401 agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag
    11461 gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg
    11521 ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa
    11581 atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat
    11641 cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga
    11701 tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa
    11761 acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact
    11821 tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg
    11881 acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc
    11941 agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt
    12001 gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt
    12061 agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac
    12121 tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga
    12181 tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc
    12241 tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag
    12301 tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg
    12361 aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag
    12421 cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat
    12481 aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc
    12541 atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc
    12601 tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca
    12661 ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata
    12721 gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa
    12781 aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc
    12841 tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg
    12901 tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa
    12961 atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct
    13021 ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt
    13081 caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc
    13141 cctaagagga tagacacgga aaggattagc aaatggttca atgactagct catctagctg
    13201 tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga
    13261 cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat
    13321 ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg
    13381 tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc
    13441 tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc
    13501 atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc
    13561 attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt
    13621 atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt
    13681 ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt
    13741 gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg
    13801 taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa
    13861 tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac
    13921 agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga
    13981 atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc
    14041 agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc
    14101 ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg
    14161 agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc
    14221 tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact
    14281 cccttttgct agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta
    14341 aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc
    14401 gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac
    14461 gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa
    14521 cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc
    14581 attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc
    14641 catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa
    14701 tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta
    14761 gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt
    14821 ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg
    14881 tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt
    14941 tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg
    15001 cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct
    15061 gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca
    15121 gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct
    15181 aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt
    15241 tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac atgacattta
    15301 atgacaaaat aacgaattat ttaatatttg taacatttaa acaaatattt ttttgataca
    15361 aaaccataaa agccttgttg tataagcaaa gtaatcgttt ttctatgttt ttttattttt
    15421 gaatgaaaaa cgcagattct gtaacattca ctgcaataat ccataaaaaa aagcaaaaaa
    15481 atttttgatt tttatcaaat tctcttaaaa aaatgtaaaa aatacaaaaa aaccagtaat
    15541 tgttacaaca attactggac aaaaactttg aataatgtta actatttttt tattttaact
    15601 ttctccgcaa taatttctgg gcatgttcca attcgtttat aacgacttct tctgtttctt
    15661 tagctaatat ttcatctaat acatcaagcc atttttccgg ttcttttgtt cctagacgtc
    15721 caatagccca agctgctgtt cctctgatga cagggcggac atctgttaaa cattctgaaa
    15781 tctcaggcaa tgccgttcga tcacctagat tagctaaagc aatcaatgcg tttctctgca
    15841 aaggtttctt cccacgccat gaaccagcta aatgcccaaa ttgctgtttg aagtctttat
    15901 tagacatcga aagcattggc tttaattttg gatatatttc gtctactttt ggttccatct
    15961 cttcatgaaa atgaaaatct tttccttgat tgtaagggca gactaactgg cagatatcac
    16021 atccataaat gacatttcgc atttttttac gatattcttc cggcatcatc ccttttgtct
    16081 gagtttgata agacaaacat cgctgggcat tcattcttcc gtcaccaagc aatgcttttg
    16141 tcggacaagc agtcacgcag cgcatacagt caccgcaacc aaattctcct ggtttatcag
    16201 gctcaaatac aatattagta acgatctcac caagatagac aaatgaaccg aactcttcag
    16261 tgatcaacaa tccgtttcgt ccaataaatc ctaaaccagc tcgctgtgct acagcgacat
    16321 cgatcagttc tccagtatct acttgcggag ctagacgcca ttcctcttct ttttgccatt
    16381 tttctgctct tgtttggatg aattcgatca atcggttcaa acggtctctg agtatatcat
    16441 ggtaatcgat cccccaagaa gcacgagcaa actgcccgcg tttttcatcc ctaggcactt
    16501 cttcatggat cctagtagga tacgcaagtg caatggaaat gatcgatttc ggctgatcaa
    16561 atgtcatttc tggataaaga cgctcatcga tatttggatg ttcaaagcca gaagtatggc
    16621 cagctgcttt ttgttcttcc aacgattctt ttaatgaatc aaaaggttcc gccgtggtga
    16681 acccgatttt gtcaattcct aatcgtttgc tttcttgtat gatttcttct tttaatgtca
    16741 tggtcttcct cgcttgctgt taacggctgt ataacccata atatagtttt tcttttttca
    16801 gctggacaaa ctggatctgc tgatcggcaa tcttcttttc ttccatccat ttttttcttg
    16861 cagcaaatac ttcccaataa tttgttgaaa ttaattttct tttgttatct aatgcattaa
    16921 aaaaattcaa tgaaactggt tttgtactta acagttcatg gagccttacc tcttcccatg
    16981 gcgaaaactc agacagatcg cgggaataca atacttcttc atagagattt ttttgctgtg
    17041 tgtacgtgaa tgtttgcggg attttcccag gcctagcaat ttctactaga cgattataaa
    17101 acatattcat taatttgctg ttggcaataa attgactttt ataataggcc gactggatct
    17161 ttttgattac tccttctgct ttgatgactg aaaataatgc aggccatgct ttgacggtta
    17221 tttcgtcgtt ttgtttaact aatacgccag gtgtgaagcc gactggagaa aggaacacaa
    17281 aataaccgat accggtattt tcatctaaag gctgaaattc atcaaacaac catttgccta
    17341 tgtacagctc ttcatctggt tctgttgtct cattttgatt tgctatgaat aatttagcta
    17401 attctgcttc aagaaattga aggtcttgat ccttgtcggt attttcctgc tggctgatca
    17461 atcgaaagac ttcttcttta atcgtatatt tatctttacg tgtaaaattc atagccacac
    17521 cttccttcct tactctaact ataacggaat ttcttcaaaa aaggtatctg tacgacgaaa
    17581 aacagcataa ttttttttca tatctcttat aaaaaatgca aggtctgctc ctcaagctag
    17641 accttgcact tttcagcttt cttttgtttg tgacaaacct tcatggatcg tttcaatatt
    17701 ccatttcatc atctgatagt aagaatctcc ttgttctcct tcttttgcta tggaatcagt
    17761 gaacagttta tcatagattt tcaaacccgt ttcttttgct acccgttcca tgcttcttga
    17821 gtctacactg gtttccacga ataaaaccgg tacttctttt gctcttatct gatcaatgat
    17881 cgctttcatt tgatcaggcg taccttgact ttctgtattg atctcccata tataagctgc
    17941 tggcaaatca tatgctcgtg aaaaatactt gaatgcccct tcacttgtta ctaataattt
    18001 tttgttctct tcgatagaag caaaactttc cttcgcttgt gtatctaatt cttttagttt
    18061 ttccacatat gcttctgcat tttttttata gagtgtcgca ttttctgcat ctttttcaga
    18121 gaatatacgt gcgatttcct ccacatattt tattccgtta gataggtcta gccaagcatg
    18181 gggatctgct tttgtatgtt cttcaccgct agttaaataa agaggttcta catttttgct
    18241 aactgcaaaa taatcttttc cttcttcttt tttagccgtt tccatcaagt tatcgaacca
    18301 gctgttacct gtttcaagat tcaaaccgtt gtataaaata acatctgcat cgcttgcctt
    18361 tttgatgtct tctggtagta cttcatattc atgcggatct gttccgactg gtacgatact
    18421 gtggatatct atttgatctg ttccgacctc ttttgccatg tccgccaaga tcgaattggt
    18481 agccacgact gacaactttt ctttacttcc ttcatccgct tggtcagaag cctgctttcc
    18541 gcaaccggct aataccaaga aactaactgc caagattaaa aagagtgatt tttttgtttt
    18601 catctatctg cttcctttct gaaataaaat tccttttgtt ggagaaaata aaaaagcaag
    18661 gataaaaaat gcggtacatg ctaacacgat cgtagctcct gatggccaat tgtaactata
    18721 actgaaaaac acaccgacaa ctgcacttag cataccgatt cctgcagctg taatgatcat
    18781 tttcagtaaa tggttcgtta gcaagtaagc cgtggcggct ggtgtgatca acatggcaat
    18841 caccaagatt gttcctactg tctgtaaact gactacagcc actaatgtca aaaagaacat
    18901 caaaagataa tggatcagcg atgtgttcag cccataagct tttgccattg tcggatcgaa
    18961 tgatgtgatc ttcaattctt tataaaagaa aaagacaaat atcaagacga ttccactaac
    19021 gagtgctgtc agcaacaaat cgctttccct aacagctaag acattcccaa ataaaatatg
    19081 atacaaatct gtcgcactct gggcatatga aatcaagata atccctaatg caaaaaagga
    19141 gctaaagaca attccaatcg ctgtatcatt tttcaatcgg ctccgctgag tcacgaaacc
    19201 aatcactgct gctgttagca tcccaaaagc agtggctcca aagatgtaac taaaaccaaa
    19261 catataagaa atggctacac ctggaagaac tgcgtgagag atagcgtctc ccatcaaaga
    19321 catccctcgt aaaataataa aagaaccgat gactccagaa gcaagtccta caagcatgga
    19381 agtgaccaaa gcgtattgca ggaattgata attcattaat ccatcaataa aggattggat
    19441 cattttttct ctcctccttt tactaaatgt ctgatgatct caccataggc caattgaagg
    19501 ttcttcgatg taaagacttc ttgtactgga ccggaagcga tcagctgttt atttaaaaga
    19561 ataacctcat cgaaatattc ttctacttta tgaagatcat gatggacgat caaaatcgtt
    19621 tttcctgaat tcttcaattc ctgcaagatg tcaaagatct ttcgttcact taacgcatca
    19681 atccctacga atggttcatc taaaaagatc cattctgctc cttgggctag agctcttgca
    19741 ataaaaactc tctgaagctg tccacccgat agttcgctga tctgtctttt ggcatattct
    19801 tccatcccta cttttttcaa agcttgtctt gcacgttctt tttctttttt cccaggtctt
    19861 tgtccaattc gtaaagatgg atatgttcct aaaagtacaa cgcctaatac atcaattgga
    19921 aaggaaagat ccaattcact tcgctgttcc acgtaggcta tttttttacg gactgagttt
    19981 accgactgtc cagaaaaggt gactttacca gtagtagctg gtatcaactc tagcaaagat
    20041 ttcataaatg ttgattttcc tgcaccgttt ggtccgataa taccggtaat tttttgtgga
    20101 tttatcgtca agttgatagt tttcaaaaca gttttccctt gataagcggc tgaaaggtct
    20161 tgaattacaa tggccgtacg ttccaaatct tacactcctt tttaggttta cctaactttt
    20221 tatttaaata tagtttaact aaaatggaaa gcattttcaa gtagaatgat gaaaaagttt
    20281 ttttcgaatt ttcctcgcat tttatgaaaa ttttctgtag aatcacatca agagttcaat
    20341 aaggctaacc taataatgaa aaacatgtgt tgaaacgttt gattattttt tcttgttgac
    20401 tcaacaacga aaggagtttt tgtatggaag aagaaaagtt aactcgaagt cttagtgccc
    20461 gtcatatcca aatgatcgca ctaggtggaa cgatcggtgt cggtctgttt atgggtgcct
    20521 cctcaaccat acgttggaca gggccctcag tgatgggagc ctatgcgatt gcaggattct
    20581 ttttgtattt gattatgcgt gctttaggcg aaatgcttta cgttgatcct tcgactggtt
    20641 cttttgcaaa ttacgcgagc gagtacatcc atcctgtggc tggctacttg accgcttgga
    20701 gtaatatttt tcaatatatt gtcgtaggaa taagtgaagt gattgctgta ggatcttata
    20761 tgaattattg gtggcctgat ttgccggcaa ttattcccgg aatcattgtc gtcttgtttc
    20821 ttatgctggc aaatcttatt tctgtcaagg cgtttggcga attagaattt tggttttcga
    20881 tcatcaaagt cataacgatc attttgatga tcattgcagg tcttggggta atcttatttg
    20941 gatttggtaa tcatggtcag gcagtaggga tttcaaatct ttggaaaaat ggtggattct
    21001 tcactggagg cgtcaaagga tttttctttg cattatcgat cgttgttgct tcctatcaag
    21061 gaatcgaact gatcggaatg actgctggtg aagcagagaa tcctaaaaag acgatcattg
    21121 aagctgtcca gtccactatc ggtcgaatct tgatttttta tattggtgct atttttatca
    21181 ttgtgagtat ctatccgtgg aatcaattga gtgagattgg ttcacctttt gtacaaactt
    21241 tttctaaagt cggcattaca tttgctgcag gactgattaa ttttgtcgtg attacagcgg
    21301 cattatcagg ttgcaattca gggattttca gcgcaagtcg tatggtctat acacttgcca
    21361 taaatggaaa gatgtctaaa aaatttctga aactatctcg tcacggtgcg cctttttatc
    21421 ctgtcgtagc aatctcaggt ggaatcttaa ttggtctgat cttaaattat cttctaccaa
    21481 tgttcatcaa ccagtcagaa aatattttcg ttttcgtata cagctcaagt attttgcctg
    21541 gtatggttcc ttgggtagtg attttgatca gcgagattcg cttcaaaaag aagcacgcag
    21601 ataaaatgca tgatcatcct tttaaaatgc cactatctcc ttatacgaat tatgtgacac
    21661 ttgctttttt agctatcgta ttgattttca tgtttatcaa tcctgaaacg agaatctcat
    21721 tgattattgg actcgttttc ttgctttaca tgacgatcca ttactttgta cgcgaaaaaa
    21781 gaaccggaaa ataaaaaaag aacgaagctt caccttagtg gaagcttcgt tcttttttat
    21841 tgcttttatc aattcaattt tagcgtctca ccgggagtca ggaaataatt ctccatgctc
    21901 atcccattca gcgacaagat cgtttctact ggtacgccag ttcgttcggc tatttgattt
    21961 ggaccttctc cttccaaaac tacggttgtc gcagcctgtg aagtcgcgct gttggtatta
    22021 gtttcagagg aatctgttgt gtcttgtgtt gttcgagctt gcatttcaga agcttctacc
    22081 gactctgctg tctcagaatc agaagatgtt gtgtcttgtg tgcttgttga ctctgcttga
    22141 agagaggcgc tagtctgcaa ataaatatcg gaaatcttag cgacatgatt cgtataatta
    22201 gccgataatt gattgatcaa agtatctaag tctacaccgt ctttattttc tgctgcctga
    22261 tacgctgcac gtaattttct gatcccttcg tcggagattg cttgaagctc acgtgctttt
    22321 tggttagcaa ctgctgataa accagctatt ccctcttgat ttccctgaat ttctgtctgg
    22381 tattcttcaa tcaatgcagg cgtcttttct tcgatctttt tcgaataatc atttacgatt
    22441 gcttgaagtt gctctggcaa accatttgct gttttttctt cagaagtttg tgtctctttt
    22501 gttttagttg atgactcact tgtttgtttc gtctctgttt tctttgtcgt tgtttccgtt
    22561 atggtcgtac ttttcgcttc tgacgaattt tttccatgaa aatatagacc cgcaactcct
    22621 cctgctatga tcaaaaacac ggcaactgct gccccaatca tgatcatccg cttacgagct
    22681 gcctgttttt gctgtcttct tcgggtcatc cgaacatttt ctccccactc attttcatca
    22741 tacatttttt tcaccaccaa tttttatgta taatagcgtt atcaaacgct tcatattctt
    22801 attgtagcaa ataattgtcg ctgtgcacat ttttatcttt gttatttata agaaaatttc
    22861 accagttgag atgattccaa gattgtccgt cgatttctcc ttgcagctgc attgttcgtt
    22921 cgtctatctc agccatcagt ttctttattt catataagag cgcacggtct tgatattctg
    22981 atttttcgct taattcatct aattgttgga tcagccaatc atttgtcata atgaagttcc
    23041 tttcatttct tgcagtgtaa aggtcaagtc ttcgatctgc tcttttgcct cagtcagtgc
    23101 ttctacgata tatgcttgtt tttcagattc ttcttcctgc attttctgat tttcctgtac
    23161 tctttgaata aaccagtcaa tccgctgagt cagcgatggt ttgccttcca atgagagata
    23221 ctgctgataa gcaattagct tctcccaacg tttttcctgg gataaataca taaattgtgc
    23281 taaaacgaat ggcggaagat aggtcatatg gaatttattt gcgattcgct gatacggcct
    23341 caaaaattcg gaaatcgtat attcttcttt tcctccagct tggtattctt tttccgccac
    23401 acctgttgtc aatacgattc ctagttcttt cccttttaat acatgtgctg ctcgttccaa
    23461 gacattatct tgccaaattt ttaaatgagc aggagaacta taccagtaaa acggaaactg
    23521 gaaaatgatc cgatcatgct gggccaagag ctgtagttca gtctctatat ctatttttcc
    23581 gtctggataa cttgattcca aatgatgcca agtagctggt aaaccagcag aagcttcttt
    23641 gaaaaattgc tgatttaaag attcttcgat cgttggatgg ctaatgatga taagtgtacg
    23701 catccctttt cctccttttt agtcttcgct ttacagtgta gcataaccgc cacaagtttc
    23761 ttttgccgat gcttgtcatt ttaaccagac taatcaggtt gtccaagaaa ctgcggtttg
    23821 tttttccaac tgttttttca aggtatccaa agcaatattc ttaaaatttt cgtttttcat
    23881 acgtttacga tagttcgtag ccgtttctcc ggtactcttt ttgaacattc ggctaaatac
    23941 gactagacta ttgaacccta cttgataggc aatactttcc acggattcat ccgtcagaga
    24001 aagaagaaac gcccctcgat tgatccgtac cccgatcaaa tattccttga tactggtacc
    24061 catatgattt ttgaatactc gcgacaaatg gctccgattg accgctaaat gctgggcgat
    24121 ttcctcaaca gacaattttt cagcataatg tgcctcgatg tatttgactg tttcaacagc
    24181 taaagaggaa tgaacctttt gtacaccacc gctgaccttt ccgccatctt ttaaaaggac
    24241 tgataaaaaa cggtaggtta atgctgttaa ctgtaattca ttttccaagg tttcttccgt
    24301 atctttcatg cattctaaaa taatgtcgat atatttttgt atgtcagaag agaccatcgt
    24361 gtattggtct tctctgaaaa gcgactgccg aatgatttct tctgcagcac tcccaccaaa
    24421 tgaaagccag caatacatcc acggatcttc tccatctgat aagtaaaacg tcgagtctcc
    24481 tggacgaatc aagaacagat cacctttttt caattggaat tgttgatttt tcacatgata
    24541 agatcctttg ccttccatca cgacatgcag gatgtaatca ttgcgaatag caggaccaaa
    24601 agaatgaaac ggcatcgttt tagagaaacc acaaaaatca aaatacaatt ctttttctac
    24661 ttgttgatgt tcctttttta aaaaaatcga ttgctccacg tctatccctc cttactcctt
    24721 tagtataatg caacaaaaag ttaaataaaa gtcacagaaa tatattaaaa aggaagcgat
    24781 ttcttgttaa gtttaagtca ttaaaagatc tttgttattc gttgtaaccg gtttacttag
    24841 tattatatat tgaacaggag aggaatacag attatgaaaa gcaaaatgat ccaacgtgtg
    24901 tcctacgctt taggcgcttt tggacacgac acattctatg ttacattaag cacttatttt
    24961 atggtattcg tgacaagcca gatgttcgca ggaacagata aggcaacgaa tgcaaaaatg
    25021 attgctacag ttacaagctt agttgtaggt atccgcttag ttgaaattat ttttgatcct
    25081 ttgattggtg gcatcatcga taatactcgt acaaaatacg gtaaattcaa accatggtta
    25141 gttatcggcg gcgttgttag ttccgtactc ttggtagcaa tctttaccaa tttctttggc
    25201 ttagctactc ataatacgac tttatttatc atattatttg caatcgtttt catcttattg
    25261 gatagttttt attcatttaa ggatatcgct ttttggtcaa tgattccagc tttgagtacg
    25321 gaaacaaagg aacgtgaaaa atttgctacg tttgctcgtt tcggctcttc tctaggtgca
    25381 aatggtacta ctctagtcgt tgtccctatc gttactttct ttacctatct tgttactcat
    25441 aaacatattg aaggcgcttc aggctggttc tggttcggtg tgattgctgc tgttatttca
    25501 ggcggtactg cgattatcac tgccttgggc acaaaagaag tcgatactgc gatccgtcag
    25561 caaaatgaaa aagctgggat caaagatgtg ttcaaagcag ttgctcaaaa tgatcagttg
    25621 atgtggcttg cccttgctta tatcgcttat gcgattgcca atgtagcaac aactgccgta
    25681 ttattctact tcttcaaata tgtacttggc caaccaactg aattttggat cgttggtcta
    25741 gttgctgcct tacttggttt agtagctgtt ccactattcc caatgcttac acgcgtcatc
    25801 tctcgtcgct acgtttacct tggagggatc gcaatgatga tttcttctta ctttttcttt
    25861 acgatcgcag gttcaagttt gccaatcgtt atcatcgggc tagtattgtt ctacttccct
    25921 caacaattga ttttcttatc cgctttgatg acgatcacag actctgttga atatggtcaa
    25981 tggaaaaatg gtatccgtaa cgaagctgtt actctatcaa ttcgcccttt actagataaa
    26041 atcgctggtg ctttgtctaa cgggatcgta gggtttgtcg ctatcgcttc tgggatgact
    26101 ggtaatgcta ctgctgctga tatcactgct catggcgtaa cgactttcaa agcttatgca
    26161 ttctatgctc ctgctgcttt gatgattttc tccgctatta tttttgcttg gaagattaca
    26221 ttgacagaga aaaaacatgc acaaatcgtt gagaaattgg aaaacaaatt ggctccagct
    26281 gatacaatgg aagatgaact tcacgatgca gtagttcctt cccattaaga aaggatcttg
    26341 aaaatgaaga ttacagaaaa agattttggc caaggttatc acttgataac cttggagaac
    26401 aaaaacaagc tagcactttc tatctctgat ttaggtgcta gaatcgtcag tttaaaatca
    26461 aatgatcgag aattagtttt gggatttgac accgctgaag aatatatcga aaaagatccg
    26521 tatatcggtg cttctatcgg tcgcaccgca gggagaatcg aaaatggccg cttttctctc
    26581 aatggaaaaa cttaccaatt agccacagat ccaaaaactg gacataatct gcatggtggt
    26641 gctcctggat ttgaattaaa aaaatggtct tatgtcattt tgaatggcga aaatgaagct
    26701 tctgtcattt ttactactac aagtcccgac ggagaacatg gttttccagg tacaatggat
    26761 gtggaaatcc gctatacttt gacaaaagat aatatctggc gagtgacctc tcgcggcacc
    26821 agtgaccaag atactttatt caatccgaca aatcatgttt acttcaactt gacgggagat
    26881 gcttctcagt caatcgatca acatgagctt tggctcaata gcgaagctta tgctcctttg
    26941 cgaacagata gtattcctat aggggtaaaa gaaaatgctg caggaagtgc tttcgatttt
    27001 caaataccta aaaaattagc gtccgttttt gctagtgatt tggatcaaaa aaatcttgtt
    27061 gacggaatcg atcatccatt tttcttaaaa gaaaccggct tgggtaaaga agccgctcgc
    27121 ttgactagcc ccgataaaag aatccaagta gatattgcta cagatgcttc tagtgtggtt
    27181 attttcactg caaatttcgg cacagaaaca ccagaaatgc gcaaccgaaa actggcacat
    27241 cacggtggca tcacatttga gactcaaaca gctccaggtg cagagcgctt ctcagcattt
    27301 ggttcgattc atttaaaagc cggatcagta tttgaaacag taacagaatt caaaatcaaa
    27361 acaagaaagg aataactcat catggaaatc aaaagaagct taaacacaaa atttgccgaa
    27421 acattcggac ctaaaaaaac tacacaatac ttctcaccag gacgcatcaa tttgattggt
    27481 gaacacacag attacaatgg aggccatgtc ttccccgctt ccatcactta tggaacgcaa
    27541 ggtgtagcag cacctcgaga cgataataaa atattagttt attccacaaa ctttgacgat
    27601 atcggtgtca ttagttttac tttagatgag ctagaatatg ataaaaaagc aaactgggca
    27661 aattacgtaa aaggaatggt ccttaaatta aaagaagctg gacactcaat cgatcatggg
    27721 tttgagttat tagtggaagg aacaatccct aatggtgcag gactttccag tagcgcttca
    27781 ttagaattat tagtaggtgt tgtattagaa gatctgtttg atttaaaaac agatcgcttg
    27841 gaactagtcc aaacaggtaa aaaagtagaa aatgaattca tcggtgtcaa ttcaggtatc
    27901 atggatcagt ttgccatcgg cttcggtgag ctagatcatg caatcttatt agatacaaat
    27961 acgctgaaat acgaaatggt tccagtgaaa ctagatggtt atgcaatcgt catcatgaat
    28021 acgaataagc ggcgggaatt ggcagattca aaatacaatg aacgccgtag cgaatgcgaa
    28081 gaagccttgg cacgtttgca aaaaactttg cctatctctt ctttaggaga cttagatgaa
    28141 gaagaatttg aatcaactat tgaccaaatc ggcgatgata cattgatacg cagagctcgc
    28201 catgccgttt ctgaaaatca gcggactttg aaggcaaaag ctgaattaga agctgggaac
    28261 ttagaagcat ttggtcaact gttaaatgat tctcaccact ctcttcgcta tgattatgaa
    28321 gtaactggga tcgaattaga tacactagta gatgctgctc aaaaacaaga aggtgtctta
    28381 ggtgcacgaa tgacaggagc tggatttggt ggctgtgcga ttgctcttgt caaagaagaa
    28441 aatatacctg ccttcaaaaa caatgtctat gatgaataca tgaaagttgt cggctatgct
    28501 ccggaatttt atgttgcaca cattggaaat gggacaacaa agatcgatcc agaagctgtt
    28561 catgtttgat aaaaaaatca acgatctcag ctaaaaacaa atagataaaa ataacctctc
    28621 agcaatttcc aaatgtgagc cttttgctcg tatttgaaaa ttgctgtttt ttattgacac
    28681 ggaaagagaa cgtgtgttct aattgtgttg aagaggtgat tgttatgtgg gcgaatcaaa
    28741 atccggtatt tgtttatgaa aaagaacctt caagagatat tttatgtatt gactgcaaat
    28801 ctttttacgc ctccgtagag tgcgttgaaa aggatcttga tccccttaca actaaattag
    28861 tagtcatgtc ctatccgtcc gattcgacag aaacacgggg tagcggatta atcttggctt
    28921 ccagtcctgc agcaaaaaaa gcttacggca tcaccaatat cagccgtgcc agagatcttc
    28981 ccttccccta tccaagtgat ttatatattg ttgccccaag gatggcctat tatatggaaa
    29041 agaacagaga aatcaattct atctataaaa aatatgcaga cgaagcgaac catcatgtct
    29101 attccgtcga tgaaagcttc cttgatgtaa caaatagcat ggcactcttt ggtaaaacaa
    29161 atgcgtacga catggctaaa ctgatccata aagatgtgtt tgagcagacc ggaatctaca
    29221 cgactatagg aattggcgat aacccgctac ttgctaaact agccttagac aatggctcaa
    29281 aagacggaaa gcaaatgttt gctgaatggc gttatgaaga tgtaccagaa aagttatgga
    29341 aaatctcaga tatcactgac ttttggggaa tcggcaaacg aacagcaaaa cgtctgaaac
    29401 aaatgggtat acattctgtt tatgatttag ctcacgcgga ttattatcac ttgaaaagca
    29461 agctaggggt catcggtact cagctttatg cccatgcttg gggcatcgat cgaagttttt
    29521 tgggacaaga atatacacca aaatcaaaaa gtatcggaaa tagtcaaatc ctagatagag
    29581 attatacaag acgagaagaa atcgagatcg ttatcaaaga aatggccgac caagtcggca
    29641 ctcgcttgag acgatctgca gcaaaaacac aagtagtcag cttatgggta ggatactctt
    29701 taggctatct tgatccaaat ggcaagaccg ggtttcacca gcaaatgaaa atcgaagcga
    29761 caaatgctag cagagatcta gcagatgctt tattgaccat tttccaccag cattaccact
    29821 accaggatat tcggcgtgta ggcgtgaact gttccgacct cgtatacgcg acaggtttac
    29881 agcttaacct ttttgaagat cctgaaaagc agataaatga agcgaaagtt gattttgttg
    29941 tcgatacgat tagaaaaaaa tacggctttc aatcaatcat ccatgcgcat agtctcttgc
    30001 aaggaggacg cgccatcgca cgtagttctc tcgttggcgg gcatgcaggt ggtatgagtg
    30061 gattagaagg tggaaaagat gagacggaca gcaaaaaaat tcattgacta taacgagtat
    30121 cgagatcggc catttggatt aaaatggggg actgcttttg cgatggaaga actagtcaac
    30181 ggtatcaaaa aaatgaagaa gaagcacgca aacagcctct tcctaaacaa caaatgacat
    30241 tgcaagaaat cgatagtata ctgacagaag cctttttatc tcgtaaagca gtaatcattc
    30301 agctcaattt aaaagatgaa tttggaagac tcttggatga ggtcgaaggg cacttcattg
    30361 gtgaagccta tgaagattac tttatgattg accatcagcc gatattgtgg gaagacgtac
    30421 ggtatattga gttaaaggaa acaaaaaaat ggtttgtgac atgagaggtt gtgacagaag
    30481 tgatcaactt caaaaaataa gaaaggattt ttcgaaaatt gcttcttaaa tttttgtgga
    30541 attttggctt atttttcgaa gaagcaactt ctggaacaac gtatattaga tatcagaggt
    30601 tgtgaaaaag tcgcttagtt ttgagtaaca agccaaggcg ctgatttcac aacctctgtc
    30661 tatctatcca tctattcggt aagattcatg catgacgtct tctttattta gtatgtagag
    30721 ccctttttct tctgaccatt caacataaat cagctgtttt acatctgcat agccttcttt
    30781 cttcaagttc tccataagcc atgcttcagt caattgattg gactgaagca ctttatgctg
    30841 aatttgccct tcatctacaa gcaggatcga taaagctttg tcttcatctg attttttcaa
    30901 tacactcatt tgaccggcat tctctaggat aacatgtttg gcattttcaa gaaaaaagca
    30961 ttcttgctgt cttaagattg ctcgcaactg ctccatctcg atatggttat ttgtcaactc
    31021 tgtcatgttg atgacgccgt ctttgattag aacagatggc tcgcctttga cccaacgatt
    31081 gaccttttca tttttttgga cgatacgttc gataccataa atcattactg cccaaagcgc
    31141 aatggcaaat aggacatgcc ctacatggac attgtcatca taggttgatt cttctaagat
    31201 cccacctaaa acaagcgtat agactaaatc aaacggtgtg atttctgaca tggatttctt
    31261 cccaagcaaa cgtaccacta ataataaacc gatcaacccg aacaccagtt tcattgtaat
    31321 tgaaaaaatc attttttctc cccctaacta tctgttaatt aaattatata tgctttctca
    31381 cgaacacgca tggaatatac aaggaagaaa tgtatccttt tactaaaaaa aggactgtga
    31441 cactgtagtg acccccaaaa gttagacttt tttggagtgg agaaatccac tccttttttt
    31501 tatgctctaa aatgatattt ttacacaacc gttacggttg ttttacgaaa ttggacg
//
LOCUS       DACDEA010000030        30272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDEA010000030 DACDEA010000000
VERSION     DACDEA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.030"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            77..1405
                     /locus_tag="IV136_001411"
     CDS             77..1405
                     /locus_tag="IV136_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8465410.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1607..2440
                     /locus_tag="IV136_001412"
     CDS             1607..2440
                     /locus_tag="IV136_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8465411.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2456..3193
                     /locus_tag="IV136_001413"
     CDS             2456..3193
                     /locus_tag="IV136_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8465412.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3348..4316)
                     /locus_tag="IV136_001414"
     CDS             complement(3348..4316)
                     /locus_tag="IV136_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8465413.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4548..5381
                     /locus_tag="IV136_001415"
     CDS             4548..5381
                     /locus_tag="IV136_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8465414.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5415..6254
                     /locus_tag="IV136_001416"
     CDS             5415..6254
                     /locus_tag="IV136_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8465415.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6280..6840
                     /locus_tag="IV136_001417"
     CDS             6280..6840
                     /locus_tag="IV136_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8465416.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7039..8760
                     /locus_tag="IV136_001418"
     CDS             7039..8760
                     /locus_tag="IV136_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8465417.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8925..10067
                     /locus_tag="IV136_001419"
     CDS             8925..10067
                     /locus_tag="IV136_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8465418.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10083..11294
                     /gene="thiI"
                     /locus_tag="IV136_001420"
     CDS             10083..11294
                     /gene="thiI"
                     /locus_tag="IV136_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8465419.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            11867..12514
                     /locus_tag="IV136_001421"
     CDS             11867..12514
                     /locus_tag="IV136_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8465420.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            12907..15552
                     /locus_tag="IV136_001422"
     CDS             12907..15552
                     /locus_tag="IV136_001422"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8465421.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            15862..17175
                     /locus_tag="IV136_001423"
     CDS             15862..17175
                     /locus_tag="IV136_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8465422.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17165..17818
                     /locus_tag="IV136_001424"
     CDS             17165..17818
                     /locus_tag="IV136_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8465423.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            17873..18565
                     /locus_tag="IV136_001425"
     CDS             17873..18565
                     /locus_tag="IV136_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8465424.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            18715..18915
                     /locus_tag="IV136_001426"
     CDS             18715..18915
                     /locus_tag="IV136_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8465425.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19169..20848)
                     /locus_tag="IV136_001427"
     CDS             complement(19169..20848)
                     /locus_tag="IV136_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8465426.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(20850..21062)
                     /locus_tag="IV136_001428"
     CDS             complement(20850..21062)
                     /locus_tag="IV136_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8465427.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21455..23185
                     /gene="efrA"
                     /locus_tag="IV136_001429"
     CDS             21455..23185
                     /gene="efrA"
                     /locus_tag="IV136_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8465428.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23182..24942
                     /gene="efrB"
                     /locus_tag="IV136_001430"
     CDS             23182..24942
                     /gene="efrB"
                     /locus_tag="IV136_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8465429.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25037..25234
                     /locus_tag="IV136_001431"
     CDS             25037..25234
                     /locus_tag="IV136_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465430.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25387..25872
                     /locus_tag="IV136_001432"
     CDS             25387..25872
                     /locus_tag="IV136_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8465431.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(25976..26569)
                     /gene="clpP"
                     /locus_tag="IV136_001433"
     CDS             complement(25976..26569)
                     /gene="clpP"
                     /locus_tag="IV136_001433"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8465432.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            26749..27276
                     /locus_tag="IV136_001434"
     CDS             26749..27276
                     /locus_tag="IV136_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8465433.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27367..28104
                     /locus_tag="IV136_001435"
     CDS             27367..28104
                     /locus_tag="IV136_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8465434.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28108..29025
                     /gene="miaA"
                     /locus_tag="IV136_001436"
     CDS             28108..29025
                     /gene="miaA"
                     /locus_tag="IV136_001436"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8465435.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29027..30256
                     /gene="hflX"
                     /locus_tag="IV136_001437"
     CDS             29027..30256
                     /gene="hflX"
                     /locus_tag="IV136_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8465436.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10009 a         5164 c         6501 g         8598 t
ORIGIN      
        1 atcatagaag agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat
       61 ttggaaggat gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca
      121 gtttcaagca aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac
      181 tgcttctgct gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt
      241 ggctacgggg aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact
      301 gaacacattc cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca
      361 aaattacact tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat
      421 tgcaacagga aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca
      481 acaagatggc ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc
      541 taaatcggtg atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt
      601 gaacaaatta ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac
      661 attagacgct attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc
      721 tatctcatta ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa
      781 ctatcatctt gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa
      841 attagatgat tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc
      901 ttatacagta gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt
      961 tgatatggaa actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg
     1021 gtttggttgt gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc
     1081 ttattcaatg gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga
     1141 ttttggagaa agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg
     1201 gcaatcaaca aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt
     1261 ctttgttatg agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga
     1321 tttattgacg aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg
     1381 ggatccaatg ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata
     1441 aaaagagctg gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg
     1501 tccctgtgct ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa
     1561 aatatgatga cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac
     1621 tgttttatct gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa
     1681 tcagacctat cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt
     1741 aaaaaaagga ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg
     1801 tgtaccggat acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg
     1861 gatcgagccg ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca
     1921 gcaaacagcg ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa
     1981 aaattctttt gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat
     2041 ttctttggca gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt
     2101 taaagaacgt gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg
     2161 agacctgtgg agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc
     2221 agctgtttct tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt
     2281 ccgaccggaa tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga
     2341 ccgactgccg atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag
     2401 ttacggttcc caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc
     2461 aaaaaaatat accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat
     2521 gacatttcct tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt
     2581 gggaagaggc agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc
     2641 catggagatt aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat
     2701 ggagtacaat aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga
     2761 attagtcaaa atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag
     2821 tgttaatgaa gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca
     2881 ggaaaaaatt gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga
     2941 tatcgatagt aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt
     3001 aggatatgac tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga
     3061 agtagagtac gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt
     3121 caaaacaaaa catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg
     3181 gaaaaataaa taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa
     3241 tttacgaaaa ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat
     3301 ttttgtcaca gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata
     3361 aacgaaatca attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt
     3421 gctttttgac tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc
     3481 ccgccttcat aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact
     3541 acagggattt tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc
     3601 cccgcaccta aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat
     3661 aagtcccctt gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa
     3721 atgtccagtc gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact
     3781 aagccgatgg gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt
     3841 gtcacgtagc gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc
     3901 gcttcttcac aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca
     3961 agttcattac ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt
     4021 gtatcaagaa aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg
     4081 gatatacctg atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc
     4141 atgatgtgtg gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg
     4201 attgtgattt ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc
     4261 atgttttctt tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta
     4321 cagacttcct tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag
     4381 agttttgcta tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta
     4441 ttaaaatgga aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc
     4501 ttttacaaga aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg
     4561 gattaacatc gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg
     4621 aatatgcagg gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa
     4681 agtcctcagt tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag
     4741 tctatcaagg aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga
     4801 tgattgcgca ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct
     4861 ttttagtcca attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca
     4921 aacttagaaa gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt
     4981 acagcgatga atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag
     5041 ccatcatcga tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca
     5101 atccgaactt tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg
     5161 ataaagagtg gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac
     5221 tagcacatga agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata
     5281 ataattctgg tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca
     5341 agccagatgg actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga
     5401 aaggggcgaa gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca
     5461 atcgttcaaa agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca
     5521 ttttatgcgg tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat
     5581 tggaattaga tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga
     5641 ttatcgacca cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga
     5701 accatcgaca aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat
     5761 taggacaatc gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac
     5821 gtctgatgaa aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa
     5881 cgttagagat attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac
     5941 agaaaaagtt agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg
     6001 tagatatcat cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg
     6061 tcgaaattga aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc
     6121 tgaaaggtgt aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg
     6181 cagactttgt gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg
     6241 gtctgattta ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt
     6301 caaaaagtag aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct
     6361 tttacagaaa atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt
     6421 gttgcagcta cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa
     6481 gcattagata aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg
     6541 gttgaacaag tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta
     6601 ctaggaatag tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta
     6661 tttccagatg gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac
     6721 tggcagatgg aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa
     6781 gaagcgattg ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga
     6841 tcagagtttc actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa
     6901 gttagtgaat gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag
     6961 acgtattatt tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg
     7021 aaaacggagg aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt
     7081 atagtcgcca ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga
     7141 ttggatgcct tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc
     7201 gatgatgtta aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt
     7261 caaaaatgga atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa
     7321 gtggaaaacc tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca
     7381 gaagctacaa tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa
     7441 ttgcgggaaa cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa
     7501 gaactgaaag aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg
     7561 caaaaacaga tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct
     7621 ggagaccctg ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg
     7681 gaagctctga tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa
     7741 caacttgaag aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca
     7801 gaagaagatc ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc
     7861 aatcttgaaa aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg
     7921 attgattcgt tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact
     7981 aaccaatcaa cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg
     8041 attgagctgg atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta
     8101 cgaggtttcc aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag
     8161 atccgtcaac acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa
     8221 gtcttagaag atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga
     8281 aaaggcgaaa aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg
     8341 aaacgttttg tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt
     8401 tttgttgcta ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac
     8461 atggaagagg tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag
     8521 aagacacatg atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat
     8581 cgttatcgtc atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc
     8641 aataaagaat atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta
     8701 gaacctggtg ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa
     8761 agcaaaaaag ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt
     8821 tttgtatatt tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga
     8881 caaccgttat aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata
     8941 acagtgcaac aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc
     9001 ggattattgg gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc
     9061 agcaagcaag aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta
     9121 cttcaggcgg aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag
     9181 aatatgggaa tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag
     9241 aacaattaaa agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg
     9301 tacgtgtcga cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg
     9361 ctgtgaataa tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg
     9421 attttcctaa aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct
     9481 cgcaatggtt gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc
     9541 cacggggaac tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag
     9601 gtggagggca agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta
     9661 tggttaaagc tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac
     9721 gcttaaaagt agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag
     9781 aaggaactga ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag
     9841 ttctcgtcca tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct
     9901 cacgtaaaca tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta
     9961 catctgctgt tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta
    10021 tgattatttt caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc
    10081 ttgtgaaata cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc
    10141 gaaaaagctt tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag
    10201 aaatcaaaat ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc
    10261 agctagttat tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc
    10321 gtgtagaaaa agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt
    10381 atactgggaa agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga
    10441 caagtaatga attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc
    10501 aagttcaaat gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt
    10561 atctatcgta tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag
    10621 gaatgcttat gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc
    10681 ggggagtgga aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac
    10741 tgcaaaaagc aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta
    10801 tcgaagtgcc atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt
    10861 ggatgacaat tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc
    10921 atggtttagt cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa
    10981 gtatggtggc tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg
    11041 ataaacttga aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc
    11101 cttttgaaga ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg
    11161 aaaaggtcca tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag
    11221 ctggcttgaa gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt
    11281 cagaattgct ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa
    11341 cgaaaaaata gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg
    11401 aataccattt atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa
    11461 attttattta gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga
    11521 aatagcgagg ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat
    11581 tgcttttcaa atttttgtga aatttcggct tatttttcga agaagttact tctggaacac
    11641 cgtgtatcag aggttgcgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
    11701 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
    11761 accgttttat ttgtctcaca cccttttcct tcctacttgt aaccttgctc ccgtgttaga
    11821 aacatgttac actaaatatg tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac
    11881 agatacactg ccaaaagcaa ctgcgaaaag attaccttta tatttacgct acttgaagat
    11941 gctggatgac tcaggtataa gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat
    12001 tccttctgca acgatccgca gagacttctc gcaattaggc gagctaggca gaagcggata
    12061 tggctatgat gtcccttttc ttatcgatgt attcaataat atccttaata ctaaagaaga
    12121 aaaacgaatc gcattagtag gatatggaaa tcttggcaaa gcattgaaac acaacaattt
    12181 tagaagaaat gaaaatctaa atatcgtgtg cgtctttgat aatgatccag cactgatcaa
    12241 tcgagtaatt gatggagaaa tgatctatcc gattgatcgt ttcgcggaga tagccaaagc
    12301 aaaaaatgtg acagtagcaa tctctacggt accaagtaaa tattcacaat ctgctattga
    12361 tgaaattgtc aaaggaaatg taaccgctat acttaatttt gcgcctgaca gagtaactgt
    12421 cccagcttat gtcaatgttc aatacattga tttgacaacc gagttgcaga cgcttattta
    12481 ctttgatgag aattactcag aagttttttc ttaggaaaat ttgagtaggc ttattgatct
    12541 tgcgagaact cgtcgtaatt ctttcaaaaa ttttttagta ctaaatgaag tggttttttc
    12601 acgaccactt gttgactaaa aaaaaaaacc attgtagaat gaagcataac gctgaacaag
    12661 aggagtaact ggcacattca ttcttagaga gagaactatc tgctgaaagg ttctcaatga
    12721 caaccagtga aggtagcttg ggagtttctg tcttgaatgg agtagagata gacgagtcgt
    12781 gatcgttaac tcacttgaga ggcaataatg attgattatt gcaatttagg tggtaccgcg
    12841 taaatcatac gtcctagaat acgaaagtat tttaggacgt tttttagttt aaaggaggaa
    12901 aacagcatga cggaagaaaa taacctatca acaaaatata atccgcaaga agttgaagct
    12961 ggacgttatc agcattggtt agaacaagat ttgtttaaac cgagcggaga taaaaaagca
    13021 aaaccttatt caatcgtaat cccgccccca aatgttactg gaaaacttca tttagggcat
    13081 gcttgggata cgaccttgca agatatgctg attcgtcaaa agagaatgca aggatatgat
    13141 actttatggc tgcctggtat ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg
    13201 aagcttagag agcaagggat ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa
    13261 gtgtgggaat ggaaagaaga gtatgcgagc cacatccgcg aacagtgggc taaaatgggc
    13321 ttatctttag attacagtag ggaacgtttt acattagatg atggattatc tgaagcagta
    13381 cgtaaagttt ttgttacctt atacgaaaaa ggtttgattt atcgtggtga atatatcatc
    13441 aactgggatc ctcaagcacg aacagctcta tctgatatcg aagttattca taaagatatc
    13501 gaaggcgcct tttatcatat gagctatgaa ctagcagatg gatcaggcgt agtagaaatt
    13561 gcgacaaccc gtccagaaac gatgttggga gatacggcga ttgctgttca tccagaagac
    13621 gagcgttatc aagcgctcat cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc
    13681 ccaatcattg cagatgaata tgtagatatg gaattcggga caggtgtagt aaaaatcact
    13741 ccagcacatg atccaaacga ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat
    13801 gttatggatg atgatggtac gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt
    13861 tttgctgctc gtaaagcaat cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc
    13921 gatacgatga tccacagtgt gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg
    13981 ctgtctactc aatggtttgt aaaaatggcg ccgttagcga aaaaagccat tgataaccaa
    14041 gaaacagatc aagcagtgga attcttccct ccaagattca atcaaacgtt tttacgttgg
    14101 atggaaaatg tccatgattg ggtaatctca cgacaattat ggtggggaca tcaaattcct
    14161 gcatggtatc acaaagaaac aggtgaaatg tatgtaggga tggaagcacc agcggatagt
    14221 gaaaactggg tacaagatcc tgatgtttta gacacttggt ttagttcagc tttgtggcct
    14281 ttctcaacaa tgggctggcc agatacggaa agcgaagatt ataagcgata cttccctact
    14341 agcactttag taaccggtta tgacatcatt ttcttctggg taagcagaat gattttccaa
    14401 agcttggaat ttactggcga acgtccattt gagaatgtct tgattcatgg attgattcgt
    14461 gacgaacaag ggcggaagat gagtaaatca ttaggtaatg gaatcgatcc gatggaagta
    14521 atcgaaaagt atggagcaga tgctctccgc tggttcttat cgaatggttc tgcaccagga
    14581 caagatgtcc gcttcagcta tgaaaaaatg gatgcttctt ggaactttat caacaaaatc
    14641 tggaatgctt cacgtttcgt tatcatgaat atcgaaggaa tgaccgtaga agagatcgat
    14701 ttatctggag aaaaatcagt ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc
    14761 gaacgagtaa ctgagctatt ttatcgtttt gaatttggtg aagcaggtcg acaactgtat
    14821 aactttatct gggatgattt ttgtgactgg tacatcgaaa tgagtaaaga agtactttat
    14881 ggagatgatg ctgtttcaaa acaaaccacc cgtagcatct tagtccatac acttgatcag
    14941 atcctacgtt tattgcatcc aatcatgcca ttcgttacag aagaaatatg ggaacatatt
    15001 cctcatcagg gaaattctct agtagtggca gaatatcctg ttgtacgtcc agagtttgat
    15061 gatgaaacag cttctaaagg gatggaagta ttgaaagaat tgattcgagc cgttcgaaat
    15121 attcgttcag aagtgaatac accattgtct aaaccaatta cgttgctaat taaaacaaac
    15181 gatgctaaaa tcgatcaatt tttagttgaa aatacaagct atatcgaacg cttctgtaat
    15241 cccgaagagt tgacgatatc aagtgagatc actgcgccag atctagcgat gtcagctgtt
    15301 ctgacaggtg cagaaatctt cttgccgtta gcaggattga tcaatattga agaagaaatc
    15361 aaacgattag aaaaagaact ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa
    15421 ctttctaatg aacgttttgt ttctaacgcg ccggaagaag tagtagaggc agagcgtgca
    15481 aaagaaaaag attatttaga gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt
    15541 actattcaat aagaatagaa aaagagagtg tgaaatcagc tagctattac taaaagctag
    15601 ctgattgaac atcgttacag gaaagagttt gagcctcact cttagcttcg attcataagg
    15661 agatgtgaca taaattttgt cgcatctctt ttttagtcta ctcttattat gtaggagaag
    15721 gagggaggtg gatcatgtat caagagtcaa taaagtagtt gataaaatcg ttcctctctc
    15781 tgtttctttt ctctttgtca tacttttatt tttcttatgt gttctatttg ttataataaa
    15841 agagaaatgg gggaattgtt gatgacgatt gaagaagcaa tagaatggat ccatagtcga
    15901 ttaccatttg gttcacgtcc tggccttgac cgagtcgaag ctttattaga agtggtaggg
    15961 catccagaga gaaaagtaaa aacgatccat atcgctggaa caaacggaaa aggttctact
    16021 gtagcttatt tgagaagcct attagaagag gctgggctga cagtaggtac atttacttca
    16081 ccttatatcg aaacgttcaa tgaacggatc gcaatcaatg gcaaaagcat tccagacaat
    16141 gatttgatcc agcttgtaaa aaaatatcag ccactgatca aacaacttga tcaatttgaa
    16201 gcagtagctg gtattactga attcgagaca ttaacagcaa tggcatttga ttattttttg
    16261 atgaagaaag tggacgtagc gattatcgaa gttggcttag gcggcttatt ggattctacc
    16321 aatgtagctg taccaatgct gacgggaata acaacgattg gacttgatca taccgaaata
    16381 cttggagata caattgaaga aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt
    16441 ccagtagtaa caggaaatat tgccccagaa gcttttactg tcatccaaca aacagctaaa
    16501 gcaaaaaatg caccgcttta tgtatacggg gaaaattata aaataaacta tctgcatcct
    16561 gatgagatgt ggggagaagt atttgaattt tttaatgaaa taggaaagat ccctcaatta
    16621 accactccgc ttttagggca acatcaagct gaaaatgcag gagtagccat cgaattgtat
    16681 tttgtttact gtcaattgga aaagctgcct ttccgagaaa aagatgtttt gaagggattg
    16741 aagaaatctc gatggccagg aagaatggag cgtttgagta acgagccatt agttgtttta
    16801 gatggtgccc ataatacgca tgcaatgaac cggcttgttg agaatttgaa aaaagaattc
    16861 aaaggatatc aaatcaacat tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg
    16921 ctagaacgat tgcagcaagt accaaatgtc catatttatt tgacgacctt tgaacatcca
    16981 aaagccatcg aattaagtga agtatcagat ttagcaagtg agagagtatc aatcgtttca
    17041 ttgtggcaat tcggattagc agatatattg gaaaagatga caaatgaaga cgtattagtt
    17101 gtgaccggtt cactttattt catttcagaa gttcgccatt tattaataca attaggagga
    17161 gatcatgaag tataatgcag tgatttttga catggatggt ttgctgttcg acacagagtt
    17221 agtgtattac gaagccacac aagtagtagc agacaagatg ggttttcctt atgataaaga
    17281 cctgtattta aaattcttag gtgtttcaga tgaagaagta tgggcgaatt atcatatgat
    17341 tttctcggaa tttggcaaaa caagtgtaca aagattcata gatgattcat accaggaaac
    17401 gcttgaacgt ttttcgcaag gagcggtaca aatgaagccc ggtgtgttgg aactcctctc
    17461 ttttttagaa gaacatcaga ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat
    17521 tgaactttta ttgaagaaga acaatctgtt tgaacgattt gagacgattg tttctgcaga
    17581 aaatgttcaa cgagcaaaac cagacccaga aattttcttg ttagctaatc agaagctagg
    17641 tacagcgaag aaagaaacat tgattttaga agattcacaa aatggtattt tagcagcttc
    17701 tgcggctgag atcccggtag tcatggtacc agatctgctt ccaccatccc aagaattgat
    17761 caaaaaaaca gctggggttg tttcttcttt acatgaaatc cctgcattgt taaaataaaa
    17821 atccatctct tttcgtatct tattttagat aagatagaaa ggagatgttt ttttgggaaa
    17881 acgattgatc aaagaagtac ctactagctc tttaccaagg gaacgtatgg aaatttatgg
    17941 cgcggaagct ttatcggatc aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc
    18001 ttatagtgtg atgtcgattg ctggaaatct gttaaaaacg tttggaggac tggcctcttt
    18061 acgacaggct actttgcatg agttagaaga gattcgaggg ataggacgcg tgaaagcaat
    18121 cgaaatcaaa gccttgatcg aattaggtcg acgaattcag acggatcatg aaagagcggc
    18181 tccaccggtt cgtaccagct acgaattggc ccagcaattg attctggaaa tgaaagatca
    18241 caaacaagaa catttggttt gtatttattt ggacacgaag aatcaagtga tcttaaaaaa
    18301 aacagtgttc atcggttcgc tgaatcagtc agtcgctcat cctagagaaa ttttccatta
    18361 tgctgtacgt tattgtgcag caaggatcgt tttagcgcat aatcatcctt ctggtaatgt
    18421 tattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat
    18481 ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga
    18541 agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac
    18601 aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta
    18661 cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa
    18721 aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat
    18781 ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa
    18841 gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac
    18901 gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag
    18961 agaacaaagc gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggtat
    19021 ttttttgtat gaaaaagtaa ataaatttaa gaggctatga aagagccacc ttgtatcaag
    19081 gaataagaac aaacaaaaat ggcttctcat gtttttcatt tgttttctct tatattcagg
    19141 acaaggtgct gatttcataa cctcttattt attcttcatc tggtgtcatt accattggca
    19201 aaatcattgg atgtctttct gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg
    19261 cttctttcaa tttatattct gaacagtcag gtccattcaa acattctctt aaagcgtgga
    19321 ataagattcg ctgagcttca ttgatcattt ctcctgattc tcgcatgtag acaaatccac
    19381 gagagagaat atctggacca gccaagattt cttttttctt gatatccact gtcgcgacag
    19441 ctaatacaag accttcttct gaaagcaggt gacggtcacg taaaacgaca ttaccaatat
    19501 caccgatccc gctaccatcg atatacacat ctccagccac aaaatgccca gcaggacgcg
    19561 cagaatcgct agttaacgct aaaacatcgc cattttccat gatgaagcag ttttctgcag
    19621 gaacacctac atcttgtgct agagaagcat gtactttcaa catgcggaat tctccgtgga
    19681 caggaataaa atattttggc ttcatcaaac gaagcattaa tttttgttct tcttgtccac
    19741 cgtgaccgga agtatggatg ttattgattt ttccgtgaat cacttctgca ccagcttcag
    19801 acagcaaatt gatcaaatga ttgacacttg ttgtattacc aggaataggt gaacttgaaa
    19861 agatcactgt atcgtctggc tggatttgga tttgacggtg agtaccattc gcgatgcggc
    19921 tcaacgcggc catcgcttct ccttgagaac ctgtacatag gatcatcgtt tcattggcag
    19981 gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc tggaactttg atatatccta
    20041 aacgttgacc gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg attttacgac
    20101 cagttgccac agcggcatct gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg
    20161 caaaaatgat acgtccttcg atttttgaaa agatcttcaa aatagaatgc ccaatcgttt
    20221 tttctgattt agtaaatgtt ggaatctcag catttgtact atcagataaa aggcacaaga
    20281 ctccttcttc cccgatacga gccattttat gcaagtttgc tggttcgcca acaggagtga
    20341 aatcgaattt gaagtctcca gtcgctacga tatttccaga aggtgttttc accacgatcc
    20401 ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac
    20461 ggatgatcgt atcttcattg atctcatgga gctctgcatc tcgaagcaag ccatgctcat
    20521 ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc atagatagga atatttgctt
    20581 gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg
    20641 cttttacttt gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca tagtcaattc
    20701 caagtaaatc gtcttccggg aatttgatcc cggcatcaat caaaatgatt tcatcttgga
    20761 attgtacccc atacgtattt ttcccgattt cacctaatcc accaatagca aagacgcctg
    20821 tctcgttatt tttgatggaa agtttcatat taaaactcca ctaatgagaa atcagcattt
    20881 tctttttcgt actctagatg acttccttct agaagttgaa cgaattcaat attgtatggg
    20941 gtattttctt ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat
    21001 tttgtttctt cccgtttagg gttgcgtact tttgtttctt gataataaac tttatagatc
    21061 attgttttgt ctcctttatc tgcgtaaagc agtgatttag ctagttgatt tttttagaca
    21121 acacaaaacg aggggacaag ccacctggtt tttcatatgc tttgactaga atactggatt
    21181 cgagtcaaag agacaagcct cgtttttgct tatctgttcg gatatttttt caaccgtacc
    21241 ttctgtataa aaaatataca acaattaatt gttattctag cataaaacag caaataagta
    21301 tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc atcccgctat aatcataatg
    21361 acttttttat gagagaagtg ttctattaat aggctcgttg tgctatcatt tttagtaatg
    21421 aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc attataccat
    21481 gcgttataag aaattcttgt ttttcaattt catatgcgta tttggtttta ttttgattga
    21541 attaggactt cctacgattt tagcacgtat gatcgatgta ggaatccgca atgatgattt
    21601 tgattatgtg aagcaacaag ggatatttat gatcgttatt actgtaatcg gtattatttt
    21661 gaatatcttg ttaggttatt ttggttcgag gattacgact aatatcgtag cagatattcg
    21721 agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt
    21781 atcttcattg atcactcgta caacaaacga tgcttatcag atcatgttgt tcctgcaaaa
    21841 tattttacgt atcggtttta tggcaccaat gatgttcatt gtcagtttgt acatggtcat
    21901 gcgtacaagt gtgacactca gtttgtatgt agtaggtgca ttacctttac tattattggc
    21961 agtcgtagcg attgctaagg tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga
    22021 taaaatcaac agtatcttgc gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt
    22081 caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat gagaattatg cttctagttc
    22141 aaaaagctta ttccgtttga tggctgttgc acaaccgggt ttttatttct tatttaatat
    22201 cgtaatggta ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt
    22261 aggagatttg attgcgttta tcgagtatat attccatgcc ttgttctcat tcatgttatt
    22321 tgctagtgtg ttcatgatgt atccaagagc agctgtttca gctggacgta tccaagaagc
    22381 cctagatgcc aaaccttcga ttgtagaaga tccaaatggt atatctgata caaaaactaa
    22441 agggtatatc gaatttcaaa atgtaacatt tgcttatcca ggccattcgc aagagccagt
    22501 gatacgaaat gtcagtttta cagcctctcc aggagagacc gttgctttta ttggaagtac
    22561 cggtagcgga aaatctacgt tgatccaatt gatccctcgc ttttatgatg tgagtgaagg
    22621 aaagatcttg atcgatggag tagacgtgag agattatcgg ctaagcaagt tgcgagataa
    22681 gattgggttt attccgcaaa aagcgctgct atttactgga acgattgcag ataatttgcg
    22741 atacgggaaa gaagatgcta cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc
    22801 ttcagaattc atttctcgaa aaccagatgg atttaatgaa catttatcag aaggtggttc
    22861 caatttttca ggtggacaaa aacaacgatt ggcaatagcc agagccatca tacgccgtcc
    22921 ggaaatctat atttttgatg acagcttttc tgcattagat tatcaaacgg atgcgaagtt
    22981 acgtgctcgt ctgaaaaaag aaacgacaga atctactgta ttgatcgtag cacaacgtgt
    23041 gggaacgatc atgcatgcag ataagatcat cgtattaaat gaaggagaag tcgtcggtat
    23101 ggggacgcat cgggagttgt tggaaaaatg cccgatttat tatgatattg ccgcttctca
    23161 attatcaaag gaggaattag catgaaaaaa accttttctt ccttaaaacg tttagctcga
    23221 tatatcagac cgtatcgtga gacttttctt ttagtgatcc tatttacagt tctgacagtg
    23281 gctttcaacg tggctttgcc atatgttaca ggacttccta ctacagaaat cagccgtaat
    23341 attgccaatg gcgaatcaat caatttttct tatatattca aatgtctcat ttggatcaca
    23401 gttgttggaa tagggtattg tatttcgcaa ttactttctg gtgttttgat gacaaatgtc
    23461 gttcaaagtg ccatgcatga tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg
    23521 gtttcatatt tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac gaatgatgta
    23581 gatgcagtca gcggtgcttt acaacaagct tttattggta tcgtaaatgc gatattggga
    23641 attacgttgg ctgcagcaat gatgttttat atccagccga tgatggcgct gatctctatg
    23701 atcatgattc caacatcgat ctggatctca aaacgagtga tcaatgcttc gcagaaatat
    23761 tttcaaagca tgcagaattc tcttggtgag ctaaacggct acgtccaaga aaatatgaca
    23821 ggttttagtg tattgaaagt atatggacgc gaaaaagaaa cattggaagg attcaaaaaa
    23881 gttaatcaca gcttgaaata ctttgggttt cgtgcagcat tcatttctgg cttgatgatg
    23941 cctctagttc aattaactgc ctatgctact tatattggta tggctgttct aggaagcttt
    24001 tatgttatta ctggggcgat cgttgtcggt caactacaag cattcatcca atatatttgg
    24061 caaatcagtc agccaatggg gaacgtgaca caattgtctt cagttttgca aagtgcatca
    24121 gcggcaacga aacgtgtatt cgagatttta gatgagccag aagaaaaaga aaacaaagta
    24181 gatgcttcac tgccagaaac gattttagga gatgtgacat tcgatcatgt cgattttgca
    24241 tatgatccga agaaaccatt aatccaagat ttgaattttg aagtgaaagc tggtcaaacc
    24301 gtagctgtgg tcggtccgac cggtgccggc aagacgacat tgatcaatct attgatgcgt
    24361 ttttatgacg tagacaaagg agcaataagg attgatggaa tcgacaccaa atcaatgagt
    24421 cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag atgcatggct ttatcaagga
    24481 acgattgcag ataatatccg cttcggaaag cttgatgcga cagattatga agtagttgat
    24541 gctgcgaaaa cagcaaacgt agatcatttt atccgaacga tgccagatgg atatgatatg
    24601 gccatcaatt ctgagggaga caatgtgtct ttaggacaaa aacagttgct gacgattgcc
    24661 cgagcagtgg tctccgatcc taaaatcttg attttagatg aagcaacaag ttcagtagac
    24721 acccgtttag aagcgttgat tcaaaaagca atggatcgcg tgatggaggg acggacaagt
    24781 ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag atctgattct agtgatggat
    24841 caaggtcaga tcattgaaaa aggaacgcat gagagtttat tggcacaagg tggattttat
    24901 gaaaaactgt ataacagcca atttgctgag gaagcagaat aagtcatata agaataaaac
    24961 agtttaaaac ttattttttg ttctctattc ttgataatgg taaagtaaag ttatcaagaa
    25021 aagtgaggac acggagatgg aacagttttt aaatgaaaaa attactgaag ccaaaagaga
    25081 agttgatcgt ttaacgcacg tctgtcagac gaagcctttt gattcagctg atttttttga
    25141 aaatgagttt caaatcgaac gcttggcagc acaaatagaa gtgtacgaat taattttaaa
    25201 aaaagtaata gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc agttaccaga
    25261 tactgtaaat agacatctgg cctgagcagg ttttacctgt tttttttctt atgaaataaa
    25321 tatatgctat gataccttta ttcaatagga ttagtttcta caaaaaatga aaggagaaaa
    25381 ataaatatgg aacggtttat agcatcaaat gccacagtga ttggtgatgt gacattgtca
    25441 gaagatgtga cgatctggta tcaagctgtt ttacgcgggg acagtaattg gataaaaata
    25501 ggacaaagaa cgaatatcca agatggtaca atcatccatg tcgatcatga tgcacctgta
    25561 gatattgcgg agaatgtgac ggtaggacat cagtgcatgc tacatggctg tacaatcgaa
    25621 aaaggggcgc tgatcgggat ggggaccacg atattgaatc atgcggttat tggtgagaat
    25681 agtttgattg gtgctggatc attagtaaca gaaggaaaag ttattcctcc taatgtcttg
    25741 gcttttggtc gcccagctaa agtgatccgt cctttgacaa aagaagaaat acaaaaaaac
    25801 aaagaaaaca tccagcatta tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag
    25861 gagcaaagat aggcagtagc atccataaaa taaaaaagga tggtcagttt gagttgacca
    25921 tcctttttta tttggtttag ataagagagc ttaaaaaagc ttcctttgaa acagattaat
    25981 ttaatgaaga actgttttcc atgacttcat cgatcaatcc atatgctttt gcttgttcag
    26041 ctgtcatata gttgtcacga tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc
    26101 gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt
    26161 cgatttcagt tgcttgccct tgagcgccgc caagaggttg atggatcatg atctcagcat
    26221 ttggtaaagc aaatcgttta ccttttgtac cagctgtcag caagaagctt cccattgaag
    26281 cagccatacc cataacaatt gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa
    26341 tagccatacc tgctgtaact gaaccaccag gagaattgat ataaagatag atatcttttt
    26401 cagaatcttg agcgtctaag aaaagcaatt gggcgatgat cgaattagca agatcatctg
    26461 ttacttggcc gcttaacatg atgatacggt ctttcaataa acgtgagtag atatcgtaag
    26521 ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat taaattcata atagagttcc
    26581 tccttgattt cgtttagaaa tagtgtacca taatttagtt tctaatacct aaaatgtagt
    26641 atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc gcataaaccc
    26701 gctttatggt atgataagca gagaaacaaa acaaggagga acaacagatt gatagttggt
    26761 attactggcc ttatcggagt gctgacaggt gctgtagttg tctatatcgg catgatactg
    26821 caaatgcgtc acttggaaaa taaagaacag cgtagacgtg aattggaatt gaaagtcaaa
    26881 gaaatcgaga cgctgaatca attaaataaa aagatcaacg agatcttgca aaaacggaca
    26941 acgctgatgg aagaatatgt cagctttgat gcatttgatg attgttatat cacgattgac
    27001 gattttgcgt atctccaatc attttcagcg caaaatagtt tttatctgcc aaattatatt
    27061 ttagacgagc tatttaagaa tatctcccat cgaaaagtga ttttatctcc agatgaaaca
    27121 atgaagattg gtggatatgc ttataaaggc ggacgcgtga ttttagagaa ttttacggat
    27181 agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga agactatgac gcgccagccg
    27241 attggtttct tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt gccgttgcac
    27301 atgagagcgt ggtaatgctt tctttttttc ttaatgatac aatagaactg aacggagtga
    27361 agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg
    27421 cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact agatgtccat
    27481 ctaagcaaag atggttattt gatcgttatg cacgacgaag aagtggaccg gacgactaac
    27541 ggtaaagggc tgattcgaga aaaaacattg gaagaactca aaaaattgaa tgcagggggc
    27601 aaattcagca aagaatttcc agcagctaaa gttcctgaat tgagagaagt gttaggactg
    27661 ctcctccaaa aaaattaccg agggatgcta atgattgaaa ttaaaactga tcagtatgaa
    27721 tatcccggta tcgaagaaaa attagccgaa acattaaaaa gtagagattg gccttttcaa
    27781 catgcatatt gcagctttca tttggattct ttggaaaaaa tcgcattact ggaaccggag
    27841 atacagctgg acttattgat gagtacttct gataaaaaga tccaactagc agcagaaacc
    27901 ccttatatag aaggagtaca tccaaaaaat acttggttgt tcgaacatcc agtccaggca
    27961 gctgattttc caaaagcaat tcgtgcatgg acgattaacg atgaagaaga aatggccaaa
    28021 tgtcaagaac tcaaaataga tggaataata actgattttc cggagaaagc tcttcggtgg
    28081 aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca
    28141 gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac tatttggagg agagatcatc
    28201 agtggtgatt cattgcaaat ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa
    28261 gaagagatga ataatgttcc gcatcatttg attgatgtga tcgaacctac tcaacgttac
    28321 tcagccgctg attttcagca ttcaggtcga caattgatta ccgagattgc gaaccgaggg
    28381 cacctaccta tcatagctgg tggtacgggt ctctatatcc aatcccttct atatgattat
    28441 cagttaggag cgacagaaga agcgaatcct gagattagaa aaaagtatga aacgttagct
    28501 gaaacaattg gttcccaagc attatggaat cagttgaaag aaaaagaccc ggcagctgct
    28561 gagaaaatcc actggaacaa tcaacgcaaa gtgataagag ctctggaagt tttagagacg
    28621 acaggacata gtatcacggc acctaaagaa gagccaaagc aattgtatga ttacctcatg
    28681 attggattag atacgaagcg tagtattctt tatgaacgaa tcaatcaaag agtcgatttg
    28741 atgctagcag aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg agaagtccag
    28801 gccagtcaag gtattggata taaagaattt ttcccttatt ttgaagggaa agaaacattg
    28861 gaagaagcaa cagaacaagt caaactacat tcgagaagat acgcgaaacg tcagttgacg
    28921 tggtttcgta accgtttgga tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa
    28981 agtcagattg agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt
    29041 gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt caatggaaga
    29101 gctagttcag ctgacgaata ccgcatcagg agaagtcgta ttcacaatta cacaaaagag
    29161 accacaagtg gatcggcaaa cgattatcgg taaagggaaa ttacaagaat tagttcaaca
    29221 agcggatgcg catgaagcgg atttgatcat ctttaatcat gagctaactc cgagacagag
    29281 ccaactgatt accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga
    29341 tatttttgcg atgcgtgctc gttcgaaaga agggaagctg caggttgaac ttgctcagtt
    29401 agaatacctg ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc ttggcggtgg
    29461 gatcggaaca agagggccag gtgaaacaaa gttagaaaca gaccgccgac atatacggaa
    29521 taaaattttt gctgttaaga aagaattgaa agaagtagaa gctcatcgag agcgcaatcg
    29581 ccaaaaaaga aaaaatagcg agatttttca gattggactt attggctaca cgaatgccgg
    29641 gaagtcaact atcttgaatc tattgacaca agcggatact tattcgaaag atcaattgtt
    29701 tgcaacgtta gatccattga ctaagagatg gcgttttgct gaaggatttg agattactgt
    29761 aacggatacc gtcgggttca tccaagacct gcctacgcaa ctgatcgatg ctttccattc
    29821 tacattagaa gaaagtcaaa atatggatct tttgctacat gtagtcgatg ctagttctcc
    29881 agatcgtatt ttacaggagc agactgtctt aaaattgatg gatgaactga atatgaaaga
    29941 aatgccgata ctgactgttt acaataaggc agaccagatc gacccagcga tgttcacgcc
    30001 tacgctattc ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga tggcgttgat
    30061 cgaagcaatc aaacgtcagc tgatggaact aatgacccct tatacacttt ctgtgccaag
    30121 caacgaagga cagaaattaa gcgagttgag aagacaaaca atggttttgg acgaagcgta
    30181 catggaagaa acaaacgaat atgaagttaa agggtttgca aaaaccaatt caaaatggtt
    30241 aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DACDEA010000031        29568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDEA010000031 DACDEA010000000
VERSION     DACDEA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.031"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            342..1451
                     /gene="pdhA"
                     /locus_tag="IV136_001438"
     CDS             342..1451
                     /gene="pdhA"
                     /locus_tag="IV136_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8465437.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1454..2431
                     /locus_tag="IV136_001439"
     CDS             1454..2431
                     /locus_tag="IV136_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8465438.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2456..4099
                     /locus_tag="IV136_001440"
     CDS             2456..4099
                     /locus_tag="IV136_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8465439.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4107..5513
                     /gene="lpdA"
                     /locus_tag="IV136_001441"
     CDS             4107..5513
                     /gene="lpdA"
                     /locus_tag="IV136_001441"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8465440.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5675..5950
                     /locus_tag="IV136_001442"
     CDS             5675..5950
                     /locus_tag="IV136_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8465441.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5956..6723
                     /locus_tag="IV136_001443"
     CDS             5956..6723
                     /locus_tag="IV136_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8465442.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6891..8720
                     /gene="typA"
                     /locus_tag="IV136_001444"
     CDS             6891..8720
                     /gene="typA"
                     /locus_tag="IV136_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8465443.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8981..10753
                     /gene="recQ"
                     /locus_tag="IV136_001445"
     CDS             8981..10753
                     /gene="recQ"
                     /locus_tag="IV136_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8465444.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10867..11169
                     /locus_tag="IV136_001446"
     CDS             10867..11169
                     /locus_tag="IV136_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8465445.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11172..12335
                     /locus_tag="IV136_001447"
     CDS             11172..12335
                     /locus_tag="IV136_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8465446.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12361..15789
                     /locus_tag="IV136_001448"
     CDS             12361..15789
                     /locus_tag="IV136_001448"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8465447.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15810..16934
                     /locus_tag="IV136_001449"
     CDS             15810..16934
                     /locus_tag="IV136_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465448.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16924..17361
                     /locus_tag="IV136_001450"
     CDS             16924..17361
                     /locus_tag="IV136_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8465449.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17345..17683
                     /locus_tag="IV136_001451"
     CDS             17345..17683
                     /locus_tag="IV136_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8465450.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17802..18362
                     /gene="rsmD"
                     /locus_tag="IV136_001452"
     CDS             17802..18362
                     /gene="rsmD"
                     /locus_tag="IV136_001452"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8465451.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18359..18850
                     /gene="coaD"
                     /locus_tag="IV136_001453"
     CDS             18359..18850
                     /gene="coaD"
                     /locus_tag="IV136_001453"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8465452.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18840..19883
                     /locus_tag="IV136_001454"
     CDS             18840..19883
                     /locus_tag="IV136_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8465453.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20042..20770
                     /locus_tag="IV136_001455"
     CDS             20042..20770
                     /locus_tag="IV136_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8465454.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20886..21131
                     /locus_tag="IV136_001456"
     CDS             20886..21131
                     /locus_tag="IV136_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8465455.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21128..21346
                     /locus_tag="IV136_001457"
     CDS             21128..21346
                     /locus_tag="IV136_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8465456.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21482..22021
                     /locus_tag="IV136_001458"
     CDS             21482..22021
                     /locus_tag="IV136_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8465457.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22014..24287
                     /locus_tag="IV136_001459"
     CDS             22014..24287
                     /locus_tag="IV136_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8465458.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24325..25371
                     /gene="holA"
                     /locus_tag="IV136_001460"
     CDS             24325..25371
                     /gene="holA"
                     /locus_tag="IV136_001460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8465459.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25460..25714)
                     /locus_tag="IV136_001461"
     CDS             complement(25460..25714)
                     /locus_tag="IV136_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8465460.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26114..26383)
                     /locus_tag="IV136_001462"
     CDS             complement(26114..26383)
                     /locus_tag="IV136_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465461.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26853..27308)
                     /locus_tag="IV136_001463"
     CDS             complement(26853..27308)
                     /locus_tag="IV136_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8465462.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27446..29374)
                     /locus_tag="IV136_001464"
     CDS             complement(27446..29374)
                     /locus_tag="IV136_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8465463.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9853 a         5160 c         6259 g         8296 t
ORIGIN      
        1 tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa caccaacgtt
       61 tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg cagatgaaat
      121 ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc ggattggcat
      181 aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa atccatgata
      241 atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc aatcgcagaa
      301 tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac aaaaaagcac
      361 cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta tatcaagcac
      421 ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc gatgatgaac
      481 tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc
      541 tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag gcaagtcagc
      601 tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat cgggatgtac
      661 ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct cgaggacatg
      721 ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt atcatcggtg
      781 cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg
      841 tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac gaagcaatca
      901 atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac gggtatgcga
      961 tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa gctgtcgcag
     1021 ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat actgtatcta
     1081 aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa acactgactt
     1141 atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt acaaaagaca
     1201 cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat ttgacggaaa
     1261 aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa gaagaaatca
     1321 aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa
     1381 acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat gaagcgaagg
     1441 agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg ccttagcact
     1501 tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga aaaacggagg
     1561 agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag tattcgatac
     1621 tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag aaggatttcg
     1681 tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg aagtagtagc
     1741 tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca tcacaattcg
     1801 ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt tagaaggttt
     1861 gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg atgcaaaagg
     1921 actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac acatgaaact
     1981 atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt tggataaagc
     2041 agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa tggtgcgtga
     2101 agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa tcattgatct
     2161 tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa aaactggtcg
     2221 cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag tagtttctga
     2281 gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt ctgcacctga
     2341 tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga
     2401 agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca aagtcatggc
     2461 ttatcaattt aaattgccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg
     2521 gttcgtcaaa ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga
     2581 caaatcagta gaagaaatcc cgtcacctgt tacaggaacg gttaaaaatg tgattgttcc
     2641 agaaggtacc gtagcaaatg ttggggatgt attagtagaa atcgatgcac caggacacga
     2701 agacaatgaa ggggacagtg gtgtagccgc agaatcccaa actccagcta aaccggctgc
     2761 tgaacccact gttgatacag aatcagctgg atcgtcaagc gaaggagtct tccaatttaa
     2821 gttaccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc
     2881 aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga
     2941 agaaattccg tcacctgtca cagggacagt taaaaacgtg attgttccag aaggcactgt
     3001 agcaaatgtt ggggatgtat tagtagaaat cgatgctcct ggacacaata gtgcaccgtc
     3061 aactagtgcg ccatcagctg aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag
     3121 tgtcgttgaa gctgccgatc caaataaacg tgtattggct atgccatctg ttcgtcaatt
     3181 tgcccgtgaa aaagatgtag atatctcaca agtaactgca actggaaaag gtggcagagt
     3241 aacaaaagag gacatcgaaa acttcttggc aggtggtcca agcagtgcac cagcgaaatc
     3301 agaagcgcca gaagcggcag caccgaaaga agcagctcct gcagcagaaa gcaaaccagc
     3361 tgcaccagca aaaccattca agtccaactt aggagacttg gaagaacgtg tagcaatgac
     3421 gccaacacgt aaagctatcg ctaaagcaat ggtcaacagt aagcatacag ctccacacgt
     3481 gacattgcat gatgaagtag aagtgtctaa actatgggat aatcggaaac gatttaaaga
     3541 agttgctgct gcaaatggta cgaaacttac tttcttgcca tatgtcgtga aagctttaac
     3601 agcgactgtt aagaaatacc cagtattgaa tgcatctatc gacgatgcaa accaagaaat
     3661 cgtttataaa cattactaca atatcggtat tgcaactgat acggatcacg gactatatgt
     3721 accaaatgtg aaagatgcag accgcaaagg aatgtttgct attgcagacg aaatcaatga
     3781 gaaagcaaaa ttagctcatg acggcaaact atctgcagaa gatatgcgca atggaacaat
     3841 tacgatcagc aatatcggat ctgtaggtgg cggttggttt acaccagtca tcaactatcc
     3901 agaagtagct attttaggtg taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg
     3961 cgaaatcgtt gtcggtcgtg tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga
     4021 tggagcaact gcgcaacaag caatgaataa tatcaaacgt cttttagctg atccagagct
     4081 attaatgatg gaaggatgat aaacaaatgg tagttggaga ttttgctgta gaattagata
     4141 cagttgtaat tggtgcggga cctggtggct atgtagctgc catccgcgct gctgaaatgg
     4201 gacaaaaagt agcaatcatt gaaagagagt atattggcgg tgtttgtttg aatgtcggct
     4261 gtattccttc aaaagcactg atagctgcag gacaccatta tcaagagtca ctagattctg
     4321 aaatgtttgg agtaactagt gaaaacgtca aactagattt tgcaaaaaca caagaatgga
     4381 aagaaaacaa agttgtccat acgttgactt ctggtgttgg tttcctatta aaaaaacata
     4441 aggttgaaac aatcgaagga gaagcgttct tcgtagatga tcacacacta cgcgtcattc
     4501 atccagattc agctcaaact tattcgttca ataacgcaat catcgcaaca ggttcccgtc
     4561 caatcgagat tccaggattt aaattcggag gacgtgtgtt agattctact ggcggattag
     4621 cattgaaaga agtacctaag aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat
     4681 tgggcggagc gtacgcaaac ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa
     4741 ttttaccaac ttacgaaaaa gatctagtaa aattagtaga agatgatttc aagaaaaaag
     4801 gcgtaacagt cgtaacgaat gcaatggcga aagaagcagt cgataacggt gacagcgtga
     4861 cagtgaaata tgcagtggac gggaaagaag aatccgttac tgctgattat gtgatggtca
     4921 ctgtaggtcg tcgtccgaat acagatgata tgggcttaga acaagcgggt gtcgaagtag
     4981 gagaacgagg attgatcacg gtggataaac aaggacgtac gaacgtacct aacatctatg
     5041 ccatcggtga tatcgttcca ggggcggcat tagcacacaa agccagttat gaagcaaaaa
     5101 tcgctgctga agcaatctct ggcaaaaaag tagctgtaga ttataaagca atgccggcag
     5161 tagccttcac tgatcctgaa ttggcttctg taggtatgac gatcaaagaa gcaaaagatg
     5221 ctggtatcga agcaaaagca tataaattcc cattttcagg gaacggtcgg gcattatcgc
     5281 ttggtaaaac agaaggattc atccgtctag ttacgacgat cgaagataac gtgcttattg
     5341 gcgcgcaaat cggtggagtc ggcgcaagtg atatggtctc tgaattagct ttagcaatcg
     5401 aatcaggaat gaatgctgaa gatatcgcct tgacgattca tcctcatcca tctcttggcg
     5461 aaatcgtcat ggatgcttcg gaattagcat taggtttacc aatccatatt tgatttgaat
     5521 aatacaggcg aaacgcaact gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa
     5581 tattattatg taaacttaaa aatatgacaa cttcttggtt tattaccact tcctatgcta
     5641 cactgaagag gaatatgaaa taggagagtt gaagatgcaa gaatatcagt atccacttga
     5701 tttagattgg acaacagacg aaatggtcat tgtaatgaag atgtgggaag atctggaacg
     5761 tgcgaatgaa aaagggataa acaatgaagc ttttttgaag acttatcagc agtttaaaac
     5821 tgttgtcaaa tccattggtg aggaaaaacg attgggacgg gaatttgaaa aagcttccgg
     5881 ttattcttta tatcgtacag tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat
     5941 gaacgggtga gataaatgga acagatgatt gatcatatca agtcctggct tgaagaagct
     6001 ggtgagaaaa tcaaatcttc tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg
     6061 gatctagtaa caaatgtcga tcgaaacatc cagaattttc taatcgaaaa aatccattcc
     6121 tttgatccag aggcgaaaat attggcagaa gaagaagggt gtaatcaatt atcggatctc
     6181 gatggaagag tctttatcat cgatcctatt gatggcacct tgaattttgt gattgaaggg
     6241 gaaaattttt gtattatgct cgctgtttat gaatccggcg ttggaaagct tggtttcatt
     6301 tatgacgtga tgagaaatga acttttttgg ggtggaaaag gaataggcgt gtataaaaat
     6361 gatgagccat tgcctagacc agcaaataaa tccttatcgg aaggcttact tggtgtgaat
     6421 agttatcttt tcggtcataa taaatatgga atccgagaaa ttgcggaaag cagcatgggg
     6481 acacgtatgt acggatgcgc tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt
     6541 gcttatattt caaaccttag cccttgggat tatgcagcgg gaaatgtctt attggaagag
     6601 tttggtatgg taacaagcgg tctttttggt actcctttga catttgaaaa aagggaatac
     6661 tatttagcag ggacttcttg tgcatatgca gaaatcgcag accttttgaa aaaagaaggc
     6721 tgaaaaaaag gtagaaatca ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct
     6781 cgaaaaaatg aaaacatttt actatatttc atgaaaaaag tctgctataa ttagcagtcg
     6841 agtaaaattt gccctgtcat aaagaaccaa taaaaaagga gttagttaca ttgaattata
     6901 gaaacgatat ccgtaacgtc gcaattattg cccacgttga ccacggaaaa acaacattag
     6961 ttgatgaact attaaaacaa tcagatacat tagacgcaca tacgcaattg caagaacgtg
     7021 cgatggactc aaatgcgcta gaaaaagaac gtggtatcac gattttagcc aaaaacacag
     7081 ctgtagacta taaaggtatt cgcgtaaaca tcatggatac tccaggacac gcggacttcg
     7141 gtggcgaagt agaacggatc atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt
     7201 atgaaggaac aatgcctcaa acacgttttg tattgaaaaa agcactggaa caacatatca
     7261 caccaatcgt tgtcgtaaac aagattgaca aaccttctgc tcgtcctgag catgtcgtag
     7321 atgaagttct tgaactattc atcgaattag gagcagatga tgatcagtta gactttcctg
     7381 tgatttatgc atctgcacta aatggtacgt caagcttgtc agatgatcca gcagatcaag
     7441 aaccaaccat ggcacctatt tttgatacaa ttattgaaaa aatccctgct cctgtcgata
     7501 acagcgacga accattacaa ttccaagttt cattacttga ctacaacgat tatgtaggtc
     7561 gtatcggaat cggacgtgta tttagaggaa caatcaaagt aggagaccaa gtagcattaa
     7621 ttaaattaga cggaacagtt aagaaattcc gtgtaacgaa attatttggt ttctttggac
     7681 taaaacgtct agagattcaa gaagcaaaag ctggagactt gattgctgtc tcaggtatgg
     7741 aagatatctt tgtcggtgaa acagttactc ctgtggatca tcaagatgcg ttacctatcc
     7801 ttcacattga cgaaccaaca ttgcaaatga ctttcttagt taataattca ccatttgccg
     7861 gtcgagaagg taaattcgta accgctcgta agattgaaga acgtttgatg gcagaacttc
     7921 aaacagatgt atccttacga gttgaaccaa caaactctcc agatgcatgg acagtttctg
     7981 gtcgtggaga attgcatttg tctatcttga ttgaaaacat gcgccgtgag ggatacgaat
     8041 tgcaagtatc tcgtccagaa gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt
     8101 ttgaacgcgt gcaaatcgat acaccagaag aatatatggg tagtgttatc gagtccttga
     8161 gcttacgtaa aggtgaaatg caagatatgg tccatacagg aaacggacaa atccgtttga
     8221 cattcttaac acctgcccgc ggtttgattg gttattcaac tgaattcctt tcaatgacac
     8281 gtggttacgg aatcatgaac catacttttg accaatatct gccaatgctt cctggtcaaa
     8341 ttggcggacg tcaccaaggg gcgcttgttt cgatcgatac aggaaaagca acgacttatt
     8401 ctatcatgag tatcgaagaa cgcggtacag tcttcgtaga accaggtaca gaagtatatg
     8461 aaggaatgat tatcggggaa aacagccgtg ataatgattt gactgtcaac atcacgaaag
     8521 caaaacaaat gacaaacgta cggtctgcta ctaaagacca aacatctgtc atcaaaaaac
     8581 caaaacaatt gacgttggaa gaatctttgg aattcttgaa tgatgatgaa tattgcgaag
     8641 taacaccaga aagcattcgc ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag
     8701 catctaaaaa gaaaaaataa tctgtaaaag gatgaagaga gaaaccagct tgttataaaa
     8761 aatagctggt ttcttttttt gtccaactct tttctgtgga gaagtcatct ttgtcaatgg
     8821 acataaattc agaaaatacg ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta
     8881 gagagttttt gtttcataat aagataatgt ttcacgaaag gagatcaact tgtccttcga
     8941 agaaaatcga agtaaaatgg caagagaaag gagcgggaag atgaacgact tattaaaaca
     9001 atactttggc tatgatgagt ttagacctgg tcaaaaagaa attatccaga aagtcattga
     9061 tcaggaaaat gtattaggaa tcatgcctac cggaagtggg aaatcaatct gttaccagct
     9121 tccagcttta ctattagatg ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa
     9181 ggatcaggtg gacgcggcga atcaattagg tatacccgcc acttttatca atagctcgtt
     9241 agatggttac gaaacagcta gacgcttcca agaaatcgat agacagcagt accgtttact
     9301 ttatatcgct cctgaacgtt ttatcatgcc ggattttatc caagcaatga agcgatggaa
     9361 cgtccgtatg atcgctattg acgaggcaca ttgtatctct cagtgggggc atgatttccg
     9421 cccaagttat ttacagatgg cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt
     9481 tgctttgaca gctacagcga ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat
     9541 tccagaaaac aaccatattc agactggttt tgaacgagaa aatctacgat ttcaagtaat
     9601 taaagatcaa aaaaaagaac aatatctaat cgaatattta aagatcaaca aaaatcaatc
     9661 tggtatcatt tatgcagcaa cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa
     9721 attcgatttt tccgtgggta gatatcatgg tggtctaaat gagaatgagc gaacagaaat
     9781 gcaagaagcc tttttatacg atcgcttgca gctgattgta gcgacaaatg catttggtat
     9841 gggtataaat aaaagcaatg tccgttttgt tatccattat caaattcctg gctctctaga
     9901 agcatactat caggaagctg gtcgggcggg aagagatggt ctttccagtg aagccatcct
     9961 gcttttctct cctcaagata tccaagtcca aaagtttttt atccagcagt cccaaagaga
    10021 agaagggcaa aagcaaaaag aatatgaaaa attaaaagcg atgacagaat acgtgtatat
    10081 tgagtcctgt cttcagcaat acatcctaaa ctattttggc gagacaagca gtccgtgcaa
    10141 ccgttgtggt aactgtttag atgacagaga aatagtagaa gtaacgacag aagcacagat
    10201 ggttctttct tgcttaaaac gtatgggtga gaactatggt aaacaaatgc tgatgaaagt
    10261 cttagcagga tcgaaagaac aaaagctgca agctttaggt tttggacatt tatctaccta
    10321 tggactgatg aaaaaccaat cgcaaaaaga aacgatgcag ttaattgaat atttgatatc
    10381 gaatggctat cttctgacca tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg
    10441 gatacaagta ttgaaaggac aagaatctgt ttatcgaaag gaacccaaga aagtccagca
    10501 gctatcggat gaagaaaccg atacactatt tgaagtgctg cgggaactgc gaacggatct
    10561 agcatcagaa gcaggcgttc ccccgtatgt cgtattttca gattccacat tgaaagaaat
    10621 gagccgaata cgtccatcaa gcagattgga gatgcttcag ataaaaggag ttggccaaag
    10681 caagcttgat aaatatggcg aagccttttt atctcggata aaaaatgcgg actctaacgt
    10741 tctggataag tagaaagaac gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt
    10801 tttttttgct ccttatgaaa atcaaatatg ttataattaa ggaattagaa tgggaggtaa
    10861 gcacagatgg attctttgtc gaaaaaactt gcgatccaag tattaaatga tgaagcagaa
    10921 cggatcaaac gattaatccg aaatcaaaac aacagtctct gtatctcaca atgcaaagcc
    10981 ttcgaagaag tggtagatac acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc
    11041 cgtataggag taatcggaaa gaacgagggc cagctgatgt taagtgagct agaacgagaa
    11101 ttgaatcagt tatatagtga ggtttaccaa gaaatttatg ataaaaaaga agttggcaag
    11161 gaggaataga cttgcctaag aaagtgaaaa aaaggcatct attagactat agcatcttga
    11221 ttccctattt aattttatgt gtattgggat tgatcatggt atatagttct acgtcgtatc
    11281 tattattaga aaacggacaa aatccgtccg catcagtcat taaccagagt attttttggg
    11341 tgcttagctt gattgctatc gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc
    11401 agcgtttgat catggcagcc atcgcagttt tgacgatttt gctgctgatt gttgtgtttt
    11461 ttggtaagga gatcaatggg gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac
    11521 ctgccgaata cctaaaaatc atttctatct ggtacctgtc tctgacttta tctaaaagac
    11581 aaaacagtgt acaaaaagat tttcttggga ctgtgaaacg tccacttgca atggttatcg
    11641 gacttactgc attagtcgct attcttcccg attttggtaa tgcggcagtt atctttttga
    11701 ttattttggt tctgctttta gccagcggag tcaattatgt gtatactttg attgtaggag
    11761 taggaggctt ttgtctaagt acttttacga tctggctgat caatatcaca aacggaaaga
    11821 tcttacccgg acgtttacag tatatctata atcgatttgc tatttaccaa aatcctttct
    11881 cagacgaatt aaataaaggg catcaactag tcaacggcta ctatgccatg ttcaacggag
    11941 gactatttgg caggggatta ggtaacagta tccagaaaaa aggattttta caagaagcac
    12001 aaaccgattt tatttatgca atcgtagtgg aagaactggg agtgattatg gggattttga
    12061 tattagcgtt gctattcttt atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag
    12121 atccgttcaa ttcgcttttg tgcattggga tcggcgcaat gtttttgatc caagtatttg
    12181 tcaatcttgg aggaattact ggcgttattc ctttgacagg gatcacgttc ccatttttaa
    12241 gtcaaggagg ctccagtttg ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca
    12301 gtgcggatga aaaacgcaag agcttaggtt tataataaaa aaagatgtag gagtggttcg
    12361 atgaaaaaag ttttagttgc taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt
    12421 acagaattag gaattaaaac tgttggaatc tatgcagaag aagatgaata ttccgtacac
    12481 cgtttcaaag cggatgaagc atatttagta ggacaaggaa aaaaaccgat tgatgcgtat
    12541 ctagatattg aagggatcat ttctatcgcg aaagaatgcg gagctgacgc tattcatccg
    12601 ggttacggtc ttttgtcgga aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc
    12661 atttttgttg gacccaaact gcatcattta gatatttttg gagataaaat caaagcaaaa
    12721 gctgcagcta tcgaagcggg gattgcttcg attccaggca cagatggacc aatcgcttct
    12781 atagacgatg ccttggagtt tgcgaaacaa tacggttatc cgattatgat caaggcagct
    12841 ctaggcggcg gcggacgcgg catgcgtgta gctcacgatg aaaagagtgc aagagagggc
    12901 tacgaacgtg cgaaaagcga agcaaaagca gcctttggta gtgatgaagt gtatgttgaa
    12961 aaatacatcg ctaatcctaa gcatatcgaa gtacagattc tcggggatac gcatggaaac
    13021 gtcatccatc tttttgaaag agattgttct gttcagcgcc gtcaccaaaa agtagtagaa
    13081 gtagcgcctt gtgtctctat gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc
    13141 caattgatga aatatgtcgg gtatgtcaac gcaggtacag tagaatttct tgttgaagga
    13201 gatgattttt actttatcga agtcaaccct cgcgtccaag tggaacatac gatcacagaa
    13261 atgattacag atattgatat cgtaacaacg caactgttga ttgctcaagg tttggatctg
    13321 cataaagaaa tcggattgcc tcagcaagaa ggaattaaat taaatggttc agccattcaa
    13381 tgccgaatca ctacagaaga cccgttgaat aactttttac cagacacagg gaaaatcgat
    13441 acgtatcgtt cacctggtgg ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc
    13501 tatgtagtga caccgtattt tgattcattg ctagtaaaag tatgtacgca tggtgctacc
    13561 tttgaaacag ccattcagaa aatggaacgt tgcttgaaag aatttagaat ccgtggagtg
    13621 aaaacaaaca ttccatttat gctcaatgtc atcacacatc ctgaattcca atcaggaaat
    13681 gcaaaaacaa ccttcattga tagtacagca acattatttg aatttcctcg actacgagac
    13741 cgcggaaaca aaacgatgaa atatatcggt gaaattactg tgaatggttt tccaggtatc
    13801 gaaagtggcg aaaaaccatt ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga
    13861 actgattacg taacggctaa gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa
    13921 tggatcaaag gacaggaaaa tttgttgttg acggatacga catttagaga cgcccatcaa
    13981 agtctgctag caactcgtgt tcggacaaag gatttcaagc agattgcacg tttgactgga
    14041 gaaggtcttc ctgaactctt ttcaagtgag atgtgggggg gagcaacatt tgatgtggct
    14101 taccgctttt tgaatgaaga tccttggcaa cggttgagaa aaattcgttc tttaatgcct
    14161 aatacgttgc tgcaaatgct tttcagaggt tcaaatgccg ttgggtattc taattatcct
    14221 gacaatgtat tggtggaatt tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga
    14281 atctttgata gtttgaactg gactccacaa atggaaaaaa gtatccaagc tgtacgggat
    14341 actggtaaaa tcgcagaggc agctatttgc tatactggcg atatcaacga tccaagtcgg
    14401 gcaaaataca atgttcagta ttataaagac atggcaaagg aactagaaca actgggtgcg
    14461 catatcattg caatcaaaga tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg
    14521 attagtgaat tgaaagaaac gacagatctg ccaatccatc tgcatactca tgataccagc
    14581 gggaatggaa tcatcactta ttctgctgct tcaaaagcag gagtagacat cgttgacgtg
    14641 gcaatgagtg caatgagtgg gaatactagt cagcctagca tgagcagctt gtattatgca
    14701 ctggtcaatg gtccacggct gccagaaatc acgattgaaa atgcacagaa attaaaccat
    14761 tattgggaag atgtgcgcat gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa
    14821 acagaagtat atatgcatga aatgccagga ggacagtatt cgaatctaca gcaacaggca
    14881 aaagctgtcg gattaggtca tcgctgggat gaaatcaaac aaatgtatca tactgtcaac
    14941 ttgatgtttg gcgatatcgt taaagtaact ccatcatcta aagtagttgg agatatggca
    15001 ctatttatgg tccaaaatga tcttacagaa gaagatattt atgaaaaagg cgaaacacta
    15061 agtttccctg aatctgttgt gactttcttc caaggagaac tagggcaacc cgttggcgga
    15121 tttcctaaaa aattgcaaaa gattattctt aaaggcagac ctgcattgaa tgaacgtcca
    15181 ggtcttttgg ctgaatcagt cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt
    15241 ggctatgaac caaaacaaga cgaagtatta agctatttga tgtatccgca agtctttttg
    15301 gactatcaaa aagcatataa tcaatttggc gatgttacat tactggacac accaacattt
    15361 ttccaaggta tacgtttggg tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt
    15421 atcattcgtt tagatgaaat cggagaaccc gatatcgaag ggaatcgcgt actgttcttc
    15481 aatttgaatg gacaaagacg tgaaatcact atcaaagata attcgatcat cagtacggtc
    15541 caaacccgat tgaaagctga accgacaaac cgagaacaaa ttggagcaac gatgtctggt
    15601 tctgttttgg atgtattagt gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg
    15661 attactgaag cgatgaaaat ggaaacggcc atcgaagcac gttttgacgg agaagtcgca
    15721 catgtatatg tgtcttcagg agacactatt tcgtctggtg accttttgat tgaagtgact
    15781 gagaaataat aagcgaaagg tggggaaaca tgaaaagact gggtttgttt tggctgattc
    15841 tgctattagt tattgtggca ttctacatgg aaccagtctt gtttccgcca aaacttgaag
    15901 tgaaggaaaa ctctgatcaa caaatatcaa accaaatgac aacgtggaaa taccaggagc
    15961 ttaaagcaac tggtttttct acttatatcg gaaaaccgat cgatcagctg gaaaaggatt
    16021 ttggccaagc ctatgatcga ttaactagcg gatttggttt tgaaacaaga tattatgaaa
    16081 ataaagctag tcatctcaca tttgaagcga acatccaaga cagcagagtc acaactgtta
    16141 aagtactgca aacagggcct gctgatatcg cgccgtttta tttagggatg acgatgcaag
    16201 atcttacgaa aatcactact atctatacta atttcacttt tgagtaccat gatacagaag
    16261 ttggaatcga actaatggaa gaggatatga attatcgtcc gctaattgct tttgataacg
    16321 acacttttgc tattttattt tttgatcaaa caacaaacgg cctattttca gttgtgtatt
    16381 tagataaaga tagtcttttg aaactattgc catatcaagt gtttggtgaa ggccttccac
    16441 attatcaagc agataaatcg gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa
    16501 gtacgacttt attaaatatg cttagaagag aagacgaact tcccatatac aatcaaacac
    16561 ttgcctttca aaaccacagt cagctgctac tacatgattt tttgacaagg cctgaggaaa
    16621 tattgtcaga ggaacgtatg gctgattggc aaaaatcgct aggcagtgct caaacgacag
    16681 tgaaattctc actgacgaat gatgaattgg agaagttaat cagtaaacaa aaattagaaa
    16741 atactagtgg gctatttatt catccggcga ttgacgctac tttttcgttt ttatattggt
    16801 acagcgatcc ttacaaccat gaccgtttca tggacccggg aacggattct gtaggcattg
    16861 ctttttctaa agaaaatatg ttagtcttat tgcaagaaga aaacggagaa agtagtgata
    16921 gcagtgatcg ttaacgaaga gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa
    16981 atgataaaaa agagtaaaac gatggcttct tatcttgaac ataaacagtc aatggaagaa
    17041 tctgcaaaag tttgccagtt gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat
    17101 gtagcggcat atggtgagta tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa
    17161 gcaaaaagac agcttgatat ggatgagcat gttgcagctt tccgatatag tgaaacacag
    17221 ctgcaaagtc tgttggatca aattgtccaa gtacttgccg aagaagtttc taatgaaatc
    17281 aaagtagatg caggtagccc gttttttgaa acgaaaaaac actttggatg cggaggtaat
    17341 tgtcatgcaa ttagacaata gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg
    17401 ggtctacagc ttaaaacaat taaagaatct gaaaaagttc ggcttgttgc attatgtctc
    17461 aagaagaatg aaatatgtcg tgctttactt aaatgaagag acattggaag aaacagaaga
    17521 acgaatccgt aaattacatt ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga
    17581 aatgaatttt gccgaaaaaa taggaaacaa aacagtatta aaagacgaag gtttcgaaat
    17641 tgaagagcta aatacagaaa tccgattagc tgaacatctt taaaaatctc ttatgagcag
    17701 tttttaaata aaaaataact tggataacga tcgatttttg atcattgtcc aagtattttt
    17761 ttggtatact agtaccagta ccatttctaa ggagtgagta gatgcggata atatcaggaa
    17821 attatggcgg gcgccgttta aaaagtttag caggggcgaa caccagacca actacagata
    17881 aagtaaaaga atccatattc aatatgattg gaccatattt tgatggagag acagtattgg
    17941 atctgtttgc aggaagcggt ggtttagcta tcgaagcagt atctagaggc tgcagtcatg
    18001 cggtatgcgt agacaaaaat taccaagcgc taaaaatcat caaagaaaac atcgaaatta
    18061 cgaaagaacc agagaaattt accacaataa aacttgatgc ggacaaagca atcgaacggc
    18121 tagcagcgga gaaacaacaa ttcgactact tgtttcttga tccgccatat gcaaaacaaa
    18181 aaataatcga tcaaatccat aaaatggaag aactgggact tttttctaaa accgcagtta
    18241 tcgtatgcga aacggataaa acagtcgaat tgccagaaac tgtggctgga ttccatcaaa
    18301 taagaaaaca gatttatggc ataacggccg taactatcta tagaaaagag ggaaatcaat
    18361 gaaacgtgca ttattcccag gaagttttga tccttttaca aaaggtcact tagatacggt
    18421 ggaacgtgcg gctaaattat tcgatgaggt agtaattggt gtttttatca atacttcaaa
    18481 gaaaagtttg tttcccccag aagaaagaat gacattgatc acaaaagctg tttctcatct
    18541 tcctaatgta aaagtcatgc atcaagaaaa tcaattgacg gtagaaacag caaaagagat
    18601 tggtgcagat gcgttgatca gaggcatacg aagtatcaaa gactttgaat atgaacgaga
    18661 gattgctcag atgaatcatc atctatatga tgaattggaa actgtcttct tattagcaaa
    18721 accagaatat agtcacgtga gttccagtat tttaaaagaa gtactgcact ttggcggaga
    18781 tgtgagcagc tacttaccgc cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa
    18841 tgaaagctaa acaatggttg aaaacagtac tgattatcat atttggaatg ttgatcattg
    18901 gatttgtagt agttcccttg ccgtattatg tagaatcacc gggagccacg atcgatctga
    18961 aagaactaat tacggtcaat gataaggaag ataaaggttc tgggtctttc tctcttacct
    19021 ctgttggtat caggcaagct acaggtttct tagctttaaa agctaaaatg accccttttg
    19081 aagaattgat cagtgaagaa gaattgactg gtggggcgac aaatgaagaa tataatcaaa
    19141 tgcaacagta ttatatggat tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg
    19201 cagacaagcc ttataaaatg gaattcaaag gcgtatatgt catgagtgta gagaaaacat
    19261 cgaatttctt tggcaagctt tcagttggag atacagtaac aaaagtagac gggaaaagct
    19321 ttcaaagtac agaagcattt atggattatg taaaaagcca aaaagttgga caaacaatcg
    19381 aaatcagtta tttgcataat ggtgaagaaa aaacagcttc tggtgatctg atcgagttac
    19441 caacagataa aaaggcagga ataggtatca cgcttactga ccataccgag atcgaatcag
    19501 atacagatgt tcgtattaat tctggctcta tcggtgggcc atcagcggga ttgatgttca
    19561 ctttagaaat ctatgaacaa gtcactggta aaaacttgcg acatggtaag caaattgcag
    19621 gaactggaac gatcaacagt gaaggagaag ttggccgaat cggaggaatc gataaaaaag
    19681 tggcaagtgc ggataaagct ggcgtagaga ttttctttgc accagatgat acgatttcag
    19741 acgatgttaa gaaagaatat cctgaaataa aaaccaacta cgaagaagca aaagctgccg
    19801 ctaaaaaatt gaattcctca atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg
    19861 actatctaga gaaaatgaaa tgaaactaaa taattaaaga ggggttgtga cacgagttct
    19921 gtcacaaccc ctctttttcg tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa
    19981 ttgacgtaat tagatgttga agggacgatt ttgccagagc tcactgtttc ttcaatttta
    20041 aatgaaagga gtaatgaatg atttttactg gtcattcttt gaaaaaagaa caatgataga
    20101 aaaaatcagc aagaaaatac tagcttcacc caaattgatc atttggatgt ctatgatagt
    20161 tcttatcttt tgtatcgcaa gtggtttttt attatcaaat ttgctatttt atagagaaac
    20221 agaagagact gctgcaaaag aaatactttc ttcgtttagt tccagtggag aagatccatc
    20281 agctgaagga aacgatgtag aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa
    20341 aggaagtgtg aaggagcctg gtatctattc cttttcttca gaagaacggg tctatgatgt
    20401 actaaagcgt gcaggtggat tattagaaga agcggatagc gatcgaatca acttttctgc
    20461 gaagatagaa gatcagcaag tcctatatat ccctgctgta ggtgaagagc tgccagaaca
    20521 tcttgatcag ttggcatcct cggaaggaaa acagcctaca gcagattccg agcctcccaa
    20581 aataaatatc aatacagcca ctccatcaga acttcagcag attcctggga tcggatcagt
    20641 caaagcgcaa gaaatcattc gatttagaga agaaaacgga tccttccaga aagtggaaga
    20701 cctgcaggag atttctggta tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac
    20761 aattaaatag aatgattcaa aatcgactgg cacgagcaat aaatggacgc taatggatac
    20821 taagaataat ccaattctaa gagcttggca ggacgaggag gtagtatttt tggataggaa
    20881 atagcttgat tgttcgcaaa gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa
    20941 ataaaatatc tgggattcat attatgaata cggctgtcag cggcaataac agtcaagctc
    21001 tacgagaacg ctttgccaaa gatgtactta gctatacagc agataaagta tttattctca
    21061 ttgggacgaa tgatctagca gagcataaac aactttctaa agaaacgtac caaaaaatct
    21121 gcagtggatg atagttcagc tgaaaaaaga ctatagttct gatcatgctt tctttatcac
    21181 gccaccagca gtcgatgaga caaaacaaaa atatcggaat aacaagttga tcaaagaata
    21241 tatctctgtt ctcacagaag tagcaaaacg ccatgattgt gaagtatcga tttttatacg
    21301 gaatttcttg aacaaatgga ttcgaagaat ttttataatg ggctaatgga tgacggactt
    21361 catttcggca gtgaagggta ggatcttttg tcagacagga tgattcgtta tatttaaaga
    21421 atattttgtt taagcaatga gtacttttta atcaacttga aaaaacggag gaatataaaa
    21481 catgttagaa aaatcttcaa aagcatgcga tacgaataaa gaaaaagtta ctgataagtc
    21541 acataatgaa cgcattcctt gggaccagta ctttatggct caggcagtac tgttatcttt
    21601 acgtagtacg tgtacacgtt tagaagtagg agcgacactc gttcgagaaa aaaggatcat
    21661 tgctggcggc tataatggag cagtcagtgg agatgtccac tgcatagatg aagggtgtta
    21721 cgtcgtggat ggacactgct tgcgaacgat ccatgcagag atgaatgcac tcctacagtg
    21781 tgcaaaactt ggtatcccga cggaaggttc agaaatctat gtcacacatt ttccatgttt
    21841 ggcctgtaca aaggctttgt tgcaagctgg tgtgaaaggg atcaattatc tccatgacta
    21901 tcgaaatgat ccttatgccc aagcgttgat cgaacaatta ggtgtatctg tacaccaagt
    21961 tgctttagac tcacagcatt tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta
    22021 gcgttttaga aaggcatgtt cgaaatcggt tgatttttcc ggcaattacg tcgatgatta
    22081 tctgtttgtt gtattacact gacaattggg gttatcgttt gcttcttgcc ggaatttttt
    22141 tatggttttc gctaaaagaa ggacgtttta tcgcgtactt gtgtattttg tgtagcataa
    22201 cggtatccat ttcttatagg ctacaagagt attcattttt agaggaaaaa acaacatctg
    22261 cgcaaattac tgtatttaca gatacgattc gtgcaaatgg taattttttg acaatggaag
    22321 gaaggttatc agagaataaa caaaagctcc agctttctta tcaagtaaac acagaagcag
    22381 aattgaaaac atggcttaac ttccgaggaa agggaataga attgactgct gaaggcgagt
    22441 tcatctctcc agcaaaaaag cggaatcttt ataattttga ccaagaagct tacttcagga
    22501 accatagaat ttctggacgg ttccaaattc aaaagattga acagatcacc atgcaaaatc
    22561 actggcgaaa ctggcttcag agaaaaagag gatcttttat ctcctttgtc caatctgaat
    22621 ttccgcaaaa aacaagtgta tatattaatt ctttattatg gggctataaa gatgcaggat
    22681 tcagagaacc agaagaaatt tttagggaga gtggtttgct gcatctgttc agtctatcgg
    22741 gtctacacat ccaattttat ttagggtggc tgtattattt atttcgacgt atcggatggc
    22801 cactacacgt cagtattatt ccgttgagtc tactaactat ctgttatgtt tctattgcag
    22861 gaagttctat ttctgttttg cgtgctagtt tgctgttttt attgcgattg ctagtcaaat
    22921 tgctgaataa gcattattcc accatggact tatttgcagt tgtcttttgg cttcttttac
    22981 tatttgatcc tcgatttctt tttcatgcgg gtggacagct tagttttttc atgagttttt
    23041 tgtttatcct gatttctttt gatggagatt ggttgaaaaa aacggcctct cacgtcctgt
    23101 ttactatgat cacgatgccg ttgattgtat ggcattttta tgagtggcct attttaggta
    23161 gtctttttac attactgatt gcaccggtat tcaagtttct ttttcttcct ggtgtgttat
    23221 ttctcacact cttcaatcgc tggctgccgg aaactttact tttgctgata gaagagggat
    23281 taatcttatt tgagcgaata atcgattttt cttcagggtc gtctctcatt ataggacgtt
    23341 tgccccttgt gctatctggg atgtatattt tcggattact gatttgtatg gataggttga
    23401 aagaacaacc tgtgaaacac ttgttttatg ttggtctttt ttcttttatt tttttgactt
    23461 tgccattttt acgcttttct tcaacgatcg cttttatcga tgtcggtcaa ggagacagta
    23521 tctttttgca aagtcctttt cgaaaagaaa cgattttgat cgatactgga ggtcagttac
    23581 actttgaaag agaaaagtgg gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg
    23641 ttccatatct aaaaggtcag gggataagtg taatcgataa actcatcttg acacacgacg
    23701 atacggatca tgtgggagaa ttacccacgt tatctcagca ttttactatc aaaaaaattt
    23761 atatcggttg gggcgctgga cgaaatgagc cattacaaag gcatttatct gaagctcaaa
    23821 aaaatggtac tgagatcatt gaaatcaaac atggggacag gatcagcggt tattatgatc
    23881 tttatgtatt gactcctttt gaaaagggag agggaagaaa tgaagattcg attggtctat
    23941 ggatggaata taacaatagg cgctttttat ttttaggaga tttgaatcaa gcaatggaaa
    24001 aacaattgct tttgatttat cctaatctga aagccgatgt agtcaaatta ggtcatcacg
    24061 gtagccgtac gtcaagtaac acagattttc tttcccatat tgaagcaaaa catgggatta
    24121 tatcctgcgg cgtaaataat cgttatggtc acccgcacag cgaagtattg gagacactag
    24181 aagaaaatca gataaaaaca tggcgaacgg atcaacaagg aatgatcacc tatcgctggc
    24241 atccagtttt tcatcctcat gggctagtgg aaacaatgat agactagtaa ttctcatctg
    24301 ctcatggtag gataattttt gaaagtggtg agtaatttga gtatacaagc agagttacaa
    24361 aaaatacgta aagaaaaact tgcatcttgt tacttgattt taggaacaga aaagtttctc
    24421 caagatcaag ttcggacaga aattgtcaaa aaaatccaat tagagggcga agatgactta
    24481 aattttttaa gttttgacat ggaaaatgca agtttagatg aggtagtagc tgaagcagag
    24541 acgttgccgt ttttcggaga tcaacggtta gtattcgtag agaatcctta ttttttaaca
    24601 ggtgaaaaag taaacaatgg aatcgaacaa aatactgatt tattgacggc ttatttgaaa
    24661 gaaccattag aatcgactgt attagtattc tttgcgccat atgaaaagtt ggatgaacgg
    24721 aaaaaagtaa cgaaacaatt gaaaaaaaca gcaattatca tcaatgtcca acagttgaat
    24781 gaaaaagaag tacgtcaata tctgatgaat accttagaga attcctcaat caaaatggaa
    24841 cgaagtgcaa ttgatttatt cttgaggttg accgacttag atttatccaa aatgatgaga
    24901 gagttacaga aattggtcct ttatggacaa aatcaacagc aaatcacggt aagagaagta
    24961 gaagaattag ttcctaaaac gttagaacat aatatttttg acatgaccca atatatttta
    25021 aaagggaaga cggaacaagc tttgcgttta tacgaagatt tagttatgca aggggaagaa
    25081 acaatcaaaa ttaatgcgat tttgctttct caattacgtt tatttttaca gacaaaattt
    25141 cttgtaaaga ttggttatca gcaggcaaat attgctgaaa cattgaaaat ccatccatat
    25201 cgtgtaaaac ttgccatgca ggaagtaaaa aaattcgatg aacatttgct ggtacagttg
    25261 tttgatcaat tagttgagat ggattatcaa atcaaaacgg gacaaatcga aaaagaatta
    25321 agttttcaac tattcgtttt gcgcacaggg cagttgttaa gaaatcgata agaagaaaga
    25381 cgagggcaaa gcatgcggga aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa
    25441 tcggccgaag ccgattcttt tatttagcga tttttttgct taaacgagat ttgtcgcggt
    25501 tagctttgtt tttgtggatt agaccttttg attcagccat atcaatagct ttaacagctt
    25561 ctttatataa cgcatctacg ttgtcagcac ctgaagctac agcatcttca aattttttga
    25621 ttgcagtacg catagcgctt gtttgagatg aatttttcac attagcgttt tcgcttgtac
    25681 gaacacgttt gattgcagat tcaatgtttg gcattagttt cacctccgat aacttaagtt
    25741 cttacgcgtg ttaccgcata attcaacatt cattattata cctaaaggca gcgtgctttg
    25801 caatatgaac atgacaagtt tttttcctta acagtctttt attaggattt tttttcagta
    25861 aaaacggata gtacaagctg aaaaaaatca aaaagacgct attgtgggag gcaatagcgt
    25921 caaaccaaac tagattttta tttatctatt taaacaaagt ttgaatggga atgtttggta
    25981 ctaagttatt atataacaaa gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat
    26041 tccctgttgt tctttgtttt tctattaaga aactgcagtt attttataac cagtatcctt
    26101 tgatcagtaa aggctattct ggtgtaccga ttattttaat gtattcatcg atatttttta
    26161 tcaacatttt ttttatgacc aactggttcc gacggttcca tttgccactt acttccagca
    26221 catatttatt taggggcaaa cgtaagatat attttgccaa atagcgatct ttaagcaggc
    26281 cattgatcac acggtcctca agctgcaagt tgatcccagt cagcatcatt ggctgttctt
    26341 gtaaaacgta cgtatcaatt aaaagacctt caaaagtttc cacaagcgtc ttcctttctt
    26401 ttcaaagtaa cctgaaaaca ggcactaatt cttccatttt gatattcgtt gaagtggatg
    26461 cgatcattct ttcttttgtc cactgtaatc tagtataaca aaaaagagca gaagagccaa
    26521 aaagaaaaaa gagcatcgaa ttctatagga agtctcattg gcacggccta aattattaca
    26581 aaacattgat taaaaaaagc ccctgcatcg caggagcatc ccctatatcg ttttaatagg
    26641 tcaatcaaaa agcagtatca aacacatttt gtatcaaatc aggaaataat agataagcta
    26701 agcccagaat caaaagtacc gctagaagaa accagccgat tgttcctatg aaatacttta
    26761 atttttcgaa cattgtatca cctcgttgtc agtatatcac ggatcagtaa attgggggtg
    26821 ttaagaatct cctaaaatag acaaaatttg ttctattttc ctaaagcaaa tacttgtaag
    26881 atacagtaag tgataccaga agcaatgacc ggtccggcgg cgattcctct taagaagaca
    26941 acacctaaaa tcgtgccgaa aacaagtgca acagtgattt gtggatcaga agcaatcaat
    27001 cctacaccct tggaagataa gacagccact agcccaccgc aaaaaacagc tacccagcca
    27061 ataggggatt taaaggcttc taatagatca cgtagaccga tttctcctgt tgcgataggg
    27121 attagaatag ccgctgtgat gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa
    27181 gggaaaaagg aacgggaggc tggtatgagc ttcataatca aaacaaaagc tgccgctatc
    27241 atcagacttt gatttttagc taaaattgct acaagtaaaa taattcctaa aaaaagccaa
    27301 ctttccatga aatatattcc tcactttctt cgaaacaatc ataacatgtt tttagaaagt
    27361 tacaaaagta ggaattcatc cttcaagacc tattcataag ctgttttttt cataaaataa
    27421 acaaaatcat aaaaaaacac tttgcttatt tgacagaaga cagccgcgct ttgtatagtg
    27481 gacaagactt acattttttt gaagaatcag aaagatcagg taaaggtctt agtaaacgca
    27541 agccattcag aataaccaat attgtgcttc cttcgtgtcc tacaactcca agaggtagat
    27601 ttaaaaattg caggaagtta gaagcaatca acaagagaat aacaagaatc gaaaaaataa
    27661 tattttgttt gatgatccgc cgcattttta aagatagccg atgactgtag acaaatttat
    27721 ttaagtcgct tttcattaag acgatgtctg cgatatccat agcggcatct gtcccttgcc
    27781 ccatagcgac acctaaagaa gcggctgcta aagcaggcgc atcattgact ccatcaccaa
    27841 ccatcgcatt tacttggtaa agttcattct cttttttgac tagttgtgtt ttttcttccg
    27901 gagtacagct tgcatagtaa tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc
    27961 cagaatggtc gccggttatc atacttgtat ggatgttttg ggatttaaag taggaaatta
    28021 cttgttgggt atttgctttt ggaatatcta gcagacctaa aacagcaatt aactgctgat
    28081 ctttagaaag ataaatcacg gttttgcctt gattttccaa acgttcgatc atttccgcaa
    28141 tctctttgct gattatcatc gtctcaggat catatgcgtg cttgccaact ttccattgcg
    28201 cacctaaata ggtagtttgt aatccaaaac cagtaatttc ttctattggt aagtattgga
    28261 tttcttcagg tattgtgcaa tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg
    28321 ttttttgttc catagcaaca agaagttgtt gtgccaaatc cttttcatca aagaaaaagc
    28381 tgtcagttac aactggtttt cctttagtga gcgttcctgt tttatcaaag gcaatcgctt
    28441 ttaattcaga aagcttttct aaatggatac cgcctttgat taaaatccca ttttttgcag
    28501 aatttgaaag agcagctaga gaagctggtg tagcagatgc gacaagcgca caaggagaag
    28561 ccacaactaa taataccatt cctcgataaa agctttcatc ccaactccaa cctaggaaaa
    28621 agtgggggag aaggatcatt agaggaacag caagcaaaat caattttaca taattatttt
    28681 cgattcgttc gataaatgaa gctgtttgag ttggcgtatt ctgcgcttct tctactaatt
    28741 ggataatctt agcaaagaca gtttcggaac tcgtttttgt tactcgaatc gtgatgggat
    28801 ttccttgatt cagcgttcct gcatacatct catcgcctaa atatttttct acaggcacag
    28861 attcaccgct taatgcagac tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta
    28921 tcggaacagc tgctccttta gcgacaaata ccaagttatc cacctttagt tgagaaacat
    28981 ctacttcttg catttgtcca ttttctttta ataattgggc tttttgtggt tgaagattca
    29041 tcaaagcagt aatttctttt tgacttttat tagttgtgta ttcctctaat gcaccgctta
    29101 aacaaaagat gaatgtcaac attgctcctt caaagtaatt accgatcaaa caggcaccaa
    29161 tagcagctaa agccatcaat aaatccacat tcagtgtccg atcattccac aattctttca
    29221 agccttccga tgtttgttta tatccaccga tgataatggc actgatataa aaaagtgggg
    29281 tgatggtaag agagaatcgg tcgaagataa aaccgaagat cataaataaa aggcagccga
    29341 ttgttgctgc aaaagctttt ttctgattag tcatcgttat tagacctcct tgtcaaaaca
    29401 aggatagcat aataaaaatg taattgataa taataacagc taaatgagaa cgataatggt
    29461 tatcatttgg tgattaagct tgatgttgtt tttattattt ataatgatta ttattaaaga
    29521 aaataaaaaa ccagaacatt tctcaaataa gagaagtgtt ctggtttt
//
LOCUS       DACDEA010000032        27710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDEA010000032 DACDEA010000000
VERSION     DACDEA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.032"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(215..1159)
                     /locus_tag="IV136_001465"
     CDS             complement(215..1159)
                     /locus_tag="IV136_001465"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8465464.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1455..2399)
                     /locus_tag="IV136_001466"
     CDS             complement(1455..2399)
                     /locus_tag="IV136_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8465465.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2620..3834
                     /locus_tag="IV136_001467"
     CDS             2620..3834
                     /locus_tag="IV136_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8465466.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3856..5871
                     /locus_tag="IV136_001468"
     CDS             3856..5871
                     /locus_tag="IV136_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8465467.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5954..7327)
                     /locus_tag="IV136_001469"
     CDS             complement(5954..7327)
                     /locus_tag="IV136_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8465468.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7353..7637)
                     /locus_tag="IV136_001470"
     CDS             complement(7353..7637)
                     /locus_tag="IV136_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8465469.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7639..9654)
                     /locus_tag="IV136_001471"
     CDS             complement(7639..9654)
                     /locus_tag="IV136_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8465470.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9833..10138)
                     /locus_tag="IV136_001472"
     CDS             complement(9833..10138)
                     /locus_tag="IV136_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8465471.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10227..11258)
                     /locus_tag="IV136_001473"
     CDS             complement(10227..11258)
                     /locus_tag="IV136_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8465472.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11465..12340
                     /locus_tag="IV136_001474"
     CDS             11465..12340
                     /locus_tag="IV136_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8465473.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12492..13334)
                     /locus_tag="IV136_001475"
     CDS             complement(12492..13334)
                     /locus_tag="IV136_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8465474.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13426..15303)
                     /locus_tag="IV136_001476"
     CDS             complement(13426..15303)
                     /locus_tag="IV136_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8465475.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15300..16721)
                     /locus_tag="IV136_001477"
     CDS             complement(15300..16721)
                     /locus_tag="IV136_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8465476.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16724..20113)
                     /locus_tag="IV136_001478"
     CDS             complement(16724..20113)
                     /locus_tag="IV136_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8465477.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20370..21761
                     /locus_tag="IV136_001479"
     CDS             20370..21761
                     /locus_tag="IV136_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8465478.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21834..22481)
                     /locus_tag="IV136_001480"
     CDS             complement(21834..22481)
                     /locus_tag="IV136_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8465479.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22489..23175)
                     /locus_tag="IV136_001481"
     CDS             complement(22489..23175)
                     /locus_tag="IV136_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465480.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23181..24254)
                     /locus_tag="IV136_001482"
     CDS             complement(23181..24254)
                     /locus_tag="IV136_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8465481.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24476..25150
                     /locus_tag="IV136_001483"
     CDS             24476..25150
                     /locus_tag="IV136_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465482.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25155..26495
                     /locus_tag="IV136_001484"
     CDS             25155..26495
                     /locus_tag="IV136_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8465483.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26644..27630)
                     /locus_tag="IV136_001485"
     CDS             complement(26644..27630)
                     /locus_tag="IV136_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8465484.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8072 a         5242 c         5030 g         9366 t
ORIGIN      
        1 ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca acagattaaa
       61 tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac cattctgttt
      121 atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca gctgttaagc
      181 agttttagga tttttagttc atattcattg tttcttataa tacagtgttc aatacttctc
      241 ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt gataattcaa
      301 caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag atatcttttt
      361 cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg ttattcaaga
      421 tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct tttttacgat
      481 caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat tcatcttggg
      541 caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca gttgtatgtg
      601 accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg acgctgcgtg
      661 ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc aatgttgttc
      721 cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata tatgtcaaaa
      781 tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc atgatttctt
      841 ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga gattgaccag
      901 gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat tcttcatagc
      961 cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa aggtccaatg
     1021 cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta gagattcctt
     1081 ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg attactactt
     1141 tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt tactttcatg
     1201 aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt tgttattata
     1261 atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa tgtttacacc
     1321 tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc
     1381 tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat atgctttagt
     1441 gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt tgaatacttc
     1501 ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc gatttcttcc
     1561 aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt ctttagaacc
     1621 agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata gaccggattg
     1681 tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt atcgatataa
     1741 tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa gctttgttgt
     1801 tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa cgaacggatc
     1861 ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg cgtcaaacga
     1921 cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc ttttgatgca
     1981 tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa tatctctgta
     2041 gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc tgtgtattcg
     2101 attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat ctctatcctt
     2161 tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc aacaaaatca
     2221 tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat atttccctgt
     2281 tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa
     2341 tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa
     2401 aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc ggtttctttc
     2461 gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt tcataaagac
     2521 tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata accacttcat
     2581 actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg
     2641 atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc agtcacgtct
     2701 gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt tgaattatta
     2761 acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt tattgctagg
     2821 aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc
     2881 ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg actaggtatt
     2941 ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta tcacggagat
     3001 gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc agctttaatg
     3061 acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt agtctacgcc
     3121 atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga aacaactgaa
     3181 cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc taaaatgatt
     3241 agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc gattaaatta
     3301 gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa tatcttaaca
     3361 atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca gacaaaagca
     3421 catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt attgattgcc
     3481 actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat ttctttttcg
     3541 ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta cgcggagagt
     3601 ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc accatatggt
     3661 ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc
     3721 agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag gacgaataga
     3781 aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa ataaatttga
     3841 tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg gtatcctgga
     3901 gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg aggttttgac
     3961 tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa gagatattac
     4021 tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta tgtagaaatt
     4081 aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt
     4141 cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg agacgaagta
     4201 aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc tggctggagt
     4261 ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa agaatgcgtc
     4321 catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg gcgagcagtg
     4381 aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac tctatgttat
     4441 ggagagtccg atgtagaagc tatggatatc gattattgct actataagca aataggaatg
     4501 acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc agaggtttct
     4561 actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa acaagctgct
     4621 ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga agccttcaag
     4681 ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat ttggtctgga
     4741 gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga aatcaataaa
     4801 cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa tactatcgtt
     4861 gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac gaaagactat
     4921 gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt gatggagcaa
     4981 acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat tgattggtct
     5041 gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa agcctatcat
     5101 tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga aaattatcat
     5161 actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga ctacttttgg
     5221 gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt
     5281 catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca gcaatcgatc
     5341 ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata tttcaaattc
     5401 tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca aacgattctt
     5461 gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga atacgaccct
     5521 actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa aagcttatgc
     5581 catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg tttgtatcca
     5641 acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt tgaaacactg
     5701 aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa agataagatc
     5761 gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag ttatcctctt
     5821 ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa
     5881 ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa gcaattttta
     5941 ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa
     6001 gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc aaggctattt
     6061 gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca gtatcggcaa
     6121 atgtagaaga agcgatttta agatctccta atactggaag gataaataat ggcagccaac
     6181 taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg ccagtcgagt
     6241 tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca ggaatgataa
     6301 ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag aagctaacaa
     6361 aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca tctaatgctg
     6421 gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg atttctgcca
     6481 acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt ccagcttgga
     6541 tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca aatccaggac
     6601 cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc gatacagtag
     6661 aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa cggtctttgt
     6721 ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg gtatgcccta
     6781 acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa
     6841 tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa tttgcagtaa
     6901 accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt gcttgacctg
     6961 taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg atcatcccta
     7021 ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc actactgctt
     7081 cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg
     7141 ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg acaaatgttt
     7201 tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg
     7261 caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa ttcaacaatt
     7321 tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat cttcgttact
     7381 ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt
     7441 atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc catatcgcta
     7501 gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc tccaagatct
     7561 gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc actttgacat
     7621 actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata tttcttcttt
     7681 tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct ttgttaaatt
     7741 tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga acaaactaat
     7801 gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca aagacatccc
     7861 tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat cgccaatatg
     7921 gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac tttcttctat
     7981 tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg
     8041 ttgatccgat aattggatca tttcttctgt taataattca gatagcatag gacgttgatc
     8101 ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga gcttttcttc
     8161 gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt cttgggcaga
     8221 aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca gctgattttt
     8281 ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat cagtaagtgt
     8341 gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt gtaagcggtc
     8401 agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt cactttttaa
     8461 gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt ttctatttct
     8521 ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa aaccaatctc
     8581 ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt ttcttgtcat
     8641 ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag gtaaataaaa
     8701 agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca tcaataattc
     8761 acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga cttctgaaga
     8821 acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga tcgatgctaa
     8881 gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa
     8941 tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat cttttaccag
     9001 ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag ctaattcatc
     9061 cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta gcaaatctct
     9121 aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc
     9181 agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta atattgcttc
     9241 tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat cttccacttc
     9301 taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa cctgaagaaa
     9361 atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca aataaagaag
     9421 atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca aaaatttttc
     9481 tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca catattcggc
     9541 aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct ctgctgcttt
     9601 ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt tcacagcgct
     9661 gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg tgtttggtac
     9721 aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa tggatacaga
     9781 aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc gatcaatgat
     9841 gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct tcaaccgctt
     9901 tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct tctacagaat
     9961 ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg ccagctaatg
    10021 attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact tcttcttctg
    10081 ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt
    10141 tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa
    10201 aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg
    10261 attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct
    10321 gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg
    10381 gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa
    10441 tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt
    10501 tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa
    10561 cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag
    10621 aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca
    10681 tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca
    10741 tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt
    10801 ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt
    10861 actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat
    10921 agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa
    10981 ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga
    11041 aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac
    11101 agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa
    11161 atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt
    11221 gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga
    11281 tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa
    11341 agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata
    11401 aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca
    11461 gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg
    11521 gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt
    11581 agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt
    11641 cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca
    11701 gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat
    11761 tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga
    11821 acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt
    11881 tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa
    11941 taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac
    12001 tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat
    12061 aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc
    12121 agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc
    12181 taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc
    12241 agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt
    12301 gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta
    12361 ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt
    12421 ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa
    12481 atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga
    12541 aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg
    12601 gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg
    12661 acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc
    12721 tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg
    12781 acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt
    12841 ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca
    12901 tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct
    12961 agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc
    13021 aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc
    13081 ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt
    13141 gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga
    13201 taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa
    13261 gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt
    13321 ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag
    13381 catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg
    13441 cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata
    13501 cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt
    13561 ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac
    13621 acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc
    13681 atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga
    13741 ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc
    13801 tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt
    13861 aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg
    13921 tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg
    13981 atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa
    14041 tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga
    14101 ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga
    14161 agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta
    14221 cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata
    14281 ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac
    14341 tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc
    14401 aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc
    14461 taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc
    14521 ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt
    14581 ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac
    14641 gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc
    14701 ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta
    14761 gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat
    14821 catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg
    14881 ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt
    14941 gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg
    15001 gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg
    15061 agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct
    15121 catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg
    15181 aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt
    15241 gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt
    15301 cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc
    15361 ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg
    15421 ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga
    15481 aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg
    15541 atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta
    15601 tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt
    15661 tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta
    15721 tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc
    15781 tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc
    15841 tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt
    15901 tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag
    15961 ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat
    16021 gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt
    16081 gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct aggctcttct
    16141 aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg
    16201 atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct
    16261 gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt
    16321 ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt
    16381 gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct
    16441 tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca
    16501 taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt
    16561 ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta
    16621 atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt
    16681 aacaagaaga gacacatact aagccaacca agttttttca tatttagctg ccccctcttt
    16741 cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga
    16801 ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt
    16861 ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat
    16921 ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt
    16981 atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata
    17041 gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca
    17101 gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat
    17161 caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat
    17221 tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg
    17281 aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg
    17341 aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac
    17401 catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg
    17461 tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag
    17521 tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat
    17581 cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt
    17641 ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat
    17701 aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa
    17761 cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg
    17821 gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg
    17881 gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag
    17941 gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg
    18001 cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt
    18061 tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac
    18121 gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg
    18181 gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga
    18241 aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt
    18301 ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc
    18361 tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca
    18421 cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta
    18481 tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga
    18541 tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca
    18601 tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa
    18661 gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga
    18721 tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat
    18781 agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc
    18841 cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta
    18901 caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc
    18961 ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg
    19021 attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc
    19081 tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat
    19141 ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt
    19201 cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt
    19261 gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg
    19321 catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat
    19381 gggtccgatc attaggatct ccattccaag aatttatacc atcccattga tttgttccgt
    19441 ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag
    19501 gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac
    19561 tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt
    19621 ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat
    19681 tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc
    19741 tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg
    19801 agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc
    19861 tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac
    19921 tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga
    19981 cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata
    20041 atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc
    20101 ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa
    20161 attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata attattatac
    20221 gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg
    20281 attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga
    20341 tcaggttgtt ggaatgaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt
    20401 ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat
    20461 ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt
    20521 cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga
    20581 tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg
    20641 gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta
    20701 agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag
    20761 gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct
    20821 ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag
    20881 aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc
    20941 aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat
    21001 ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct
    21061 taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact
    21121 attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc
    21181 agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg
    21241 aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg
    21301 atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga
    21361 tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga
    21421 atctgattta ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc
    21481 accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc
    21541 aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa
    21601 ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta
    21661 cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat
    21721 tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat
    21781 gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt
    21841 attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc
    21901 caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg
    21961 aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc
    22021 catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca
    22081 tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag
    22141 caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat
    22201 tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct
    22261 tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt
    22321 tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg
    22381 attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct
    22441 cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt
    22501 tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat
    22561 catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac
    22621 tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc
    22681 taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc
    22741 accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa
    22801 ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg
    22861 ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt
    22921 aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac
    22981 tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc
    23041 tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc
    23101 tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt
    23161 taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc
    23221 gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc
    23281 aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa
    23341 taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact
    23401 aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca
    23461 gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac
    23521 aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca
    23581 atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta
    23641 gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt
    23701 tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg
    23761 taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg
    23821 atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat gatttcattc
    23881 tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg
    23941 ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt
    24001 ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca
    24061 aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct
    24121 tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca
    24181 atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg
    24241 atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt
    24301 gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat
    24361 aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag
    24421 ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat
    24481 taaactatta gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc
    24541 tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag
    24601 tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt
    24661 gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga
    24721 tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc
    24781 tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct
    24841 ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt
    24901 cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt
    24961 tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt
    25021 tgattctgat acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct
    25081 gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca
    25141 aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga
    25201 caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact ttacagatat
    25261 taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc
    25321 gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta aacccgactc
    25381 aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt
    25441 ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg
    25501 gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc
    25561 aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga
    25621 aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc
    25681 aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt
    25741 ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc
    25801 tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt
    25861 taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg
    25921 aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt
    25981 cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga
    26041 tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag
    26101 atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa
    26161 aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg aatccagaag
    26221 ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac gtaaagaata
    26281 ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt tatcgggaag
    26341 atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt gcaaaacagg
    26401 taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa ggagtcgatt
    26461 ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa aagggctgtg
    26521 acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag aagtagcttc
    26581 ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct ctttagctta
    26641 cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa
    26701 ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt ctttatgaat
    26761 cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt ctttgattgg
    26821 gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt gatctgttac
    26881 tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg tttcagtatc
    26941 gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg tatttacatc
    27001 taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc gatcgatgtc
    27061 ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc
    27121 tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat
    27181 tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg ggctaacagt
    27241 atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt ttactgtgat
    27301 gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat tttctggtgt
    27361 agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt cccataaaga
    27421 acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac cagacaatcc
    27481 gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat catctcgttc
    27541 atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg tactatagct
    27601 tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat agatttatga
    27661 ttggtatatt gttacttata aatttattct ataggagacg attgttttct
//
LOCUS       DACDEA010000033        25636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDEA010000033 DACDEA010000000
VERSION     DACDEA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.033"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..2140
                     /locus_tag="IV136_001486"
     CDS             80..2140
                     /locus_tag="IV136_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465485.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2223..2792
                     /locus_tag="IV136_001487"
     CDS             2223..2792
                     /locus_tag="IV136_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465486.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2967..3773)
                     /locus_tag="IV136_001488"
     CDS             complement(2967..3773)
                     /locus_tag="IV136_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465487.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3911..5425
                     /locus_tag="IV136_001489"
     CDS             3911..5425
                     /locus_tag="IV136_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8465488.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5828..6811)
                     /locus_tag="IV136_001490"
     CDS             complement(5828..6811)
                     /locus_tag="IV136_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8465489.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6816..7463)
                     /locus_tag="IV136_001491"
     CDS             complement(6816..7463)
                     /locus_tag="IV136_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8465490.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7548..8180)
                     /locus_tag="IV136_001492"
     CDS             complement(7548..8180)
                     /locus_tag="IV136_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8465491.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8196..8747)
                     /gene="coaC"
                     /locus_tag="IV136_001493"
     CDS             complement(8196..8747)
                     /gene="coaC"
                     /locus_tag="IV136_001493"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8465492.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8744..9481)
                     /locus_tag="IV136_001494"
     CDS             complement(8744..9481)
                     /locus_tag="IV136_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8465493.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9478..10467)
                     /locus_tag="IV136_001495"
     CDS             complement(9478..10467)
                     /locus_tag="IV136_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8465494.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10725..11267
                     /locus_tag="IV136_001496"
     CDS             10725..11267
                     /locus_tag="IV136_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8465495.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11284..12369
                     /locus_tag="IV136_001497"
     CDS             11284..12369
                     /locus_tag="IV136_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465496.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12381..13187
                     /locus_tag="IV136_001498"
     CDS             12381..13187
                     /locus_tag="IV136_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465497.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13188..14015
                     /locus_tag="IV136_001499"
     CDS             13188..14015
                     /locus_tag="IV136_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465498.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14012..15085
                     /locus_tag="IV136_001500"
     CDS             14012..15085
                     /locus_tag="IV136_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465499.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15513..18209
                     /locus_tag="IV136_001501"
     CDS             15513..18209
                     /locus_tag="IV136_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8465500.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18286..18774
                     /locus_tag="IV136_001502"
     CDS             18286..18774
                     /locus_tag="IV136_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8465501.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18786..19280
                     /locus_tag="IV136_001503"
     CDS             18786..19280
                     /locus_tag="IV136_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8465502.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19301..20110
                     /locus_tag="IV136_001504"
     CDS             19301..20110
                     /locus_tag="IV136_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8465503.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20107..20928
                     /locus_tag="IV136_001505"
     CDS             20107..20928
                     /locus_tag="IV136_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8465504.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20979..23078
                     /locus_tag="IV136_001506"
     CDS             20979..23078
                     /locus_tag="IV136_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8465505.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23075..24193
                     /locus_tag="IV136_001507"
     CDS             23075..24193
                     /locus_tag="IV136_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8465506.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24232..25110
                     /locus_tag="IV136_001508"
     CDS             24232..25110
                     /locus_tag="IV136_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8465507.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25301..25453
                     /locus_tag="IV136_001509"
     CDS             25301..25453
                     /locus_tag="IV136_001509"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465508.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8101 a         4457 c         5424 g         7654 t
ORIGIN      
        1 gattttattt atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag
       61 tagggaggac taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc
      121 ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg
      181 cacgatgctg atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac
      241 acaaaccatc aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat
      301 tactcatact ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa
      361 taaagtcagt tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg
      421 gacatcggaa accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac
      481 aaaaggtgat cagttaaaac agatcactga tattttgaat catgctttga cagatataaa
      541 ggacggtgga agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag
      601 aaagaaaatc gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct
      661 gaagaacgca gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat
      721 cgtggatacg actaatacag gaacatctac tatttccttg tcctattttg atccaattgc
      781 tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa
      841 aggaaaagat cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata
      901 tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt
      961 agatactaca actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc
     1021 aaatgtggca gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt
     1081 catcactcat tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag
     1141 tagtgtaaat cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa
     1201 tatagattta tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca
     1261 agcaaacgga ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga
     1321 actgatgaat aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc
     1381 agacaatcgt tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa
     1441 agaagagttg atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt
     1501 atcggttaca atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga
     1561 ttttacgatt atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc
     1621 actattacat gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga
     1681 agaaagcttg tcagaaaaaa aacaacaaat ggcccatgca gtttctccaa cagggaaagt
     1741 aaaacaaaca agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa
     1801 aacgataagg caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt
     1861 agagcctaaa ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca
     1921 gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac
     1981 cgatgagcat ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg
     2041 aggtattcct tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata
     2101 cgctaatcga aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat
     2161 agtcaaataa gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa
     2221 aaatgttaga taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa
     2281 atgttccaat cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg
     2341 aatctgcttg ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac
     2401 ttgagtattt tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt
     2461 ttgtagagac aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa
     2521 gagagcaagt gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag
     2581 ggctcgcaaa agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg
     2641 gaccaatcta tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa
     2701 tgggttttac ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag
     2761 ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt
     2821 gggataaaaa acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga
     2881 taactaatgg catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg
     2941 ccccttctct tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg
     3001 atgaacgtaa atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca
     3061 actgctccaa taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag
     3121 taaccatttt ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt
     3181 gtccgagtga tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta
     3241 ttgatttgct ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa
     3301 aattccacaa actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct
     3361 agtaataaag gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa
     3421 aagccgacac ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa
     3481 aaatagccat aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac
     3541 aaaatgattt ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg
     3601 atttcaaata aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag
     3661 acctgatcca ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg
     3721 attctacctg cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa
     3781 ctccttcatg tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact
     3841 taaagaaaaa cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag
     3901 ggggatacaa atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat
     3961 gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc
     4021 tgccgaagta tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga
     4081 atcttatgat gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc
     4141 aaaagatgct gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac
     4201 atctaaatca tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat
     4261 caaagactcg aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga
     4321 taaagaaatg cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca
     4381 aaacggtatc aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca
     4441 tcgtccgtct gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt
     4501 ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg
     4561 ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg
     4621 aaaagcaatc gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata
     4681 tcgtcccgat ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat
     4741 caccatgatt gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc
     4801 aactgtgcag caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat
     4861 cttggtcgat caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc
     4921 tgcagcaatt acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg
     4981 tgatcgtgcc acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac
     5041 aatggaagaa ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga
     5101 tggatggaat acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat
     5161 ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta
     5221 cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc
     5281 aattaggggg ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat
     5341 cggtggaaat tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc
     5401 tcgatttgct tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag
     5461 cgttttgagc taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa
     5521 aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
     5581 acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga
     5641 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     5701 agcagaagaa attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     5761 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag
     5821 catgatttta ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat
     5881 attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct
     5941 tgccattttc tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat
     6001 actgatcact tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata
     6061 caccaaggtc tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc
     6121 catcaacggc tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa
     6181 taacatcgac tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt
     6241 tatagttgat caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc
     6301 ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc
     6361 ccacaccact tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc
     6421 agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt
     6481 ctagcgtatc agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac
     6541 cacctagatt gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt
     6601 tattctcaac tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag
     6661 agactgattc tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca
     6721 gtaattcgcc ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag
     6781 aaccaggatg cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg
     6841 aatgctgtat tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc
     6901 gtttcttcca ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat
     6961 gccttctcat taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg
     7021 actggttgaa gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt
     7081 ttagtaatat aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca
     7141 gcactgacca tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt
     7201 gcgccatcca agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat
     7261 acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag
     7321 acatacgaaa cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca
     7381 aaaatttcat gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt
     7441 gcacctacaa caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa
     7501 tagtaacacc aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga
     7561 gaagggtgct tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg
     7621 acgattcctt caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca
     7681 tttaaatttg ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc
     7741 atcactaaaa tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa
     7801 tctgcttttt gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca
     7861 atcaagatgc gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg
     7921 atgactggga taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg
     7981 atcatgctga tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg
     8041 atcgaagcaa tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata
     8101 aaacctaaag gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta
     8161 aagttttttg tatttttcat aagtactcct tttttctaag catttctttc attaagagtc
     8221 agcatgacct gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca
     8281 caagctaaaa gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc
     8341 agattacgct gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat
     8401 tttggtgttc ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg
     8461 tttgctagct tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt
     8521 tttgctagtt cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg
     8581 actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta
     8641 gacataatca catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt
     8701 ttgtaagcag aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca
     8761 gtctaatttt ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg
     8821 ttccatcttt agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat
     8881 ttgcgaaaat aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag
     8941 attcctttgg tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt
     9001 cacgtagata agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt
     9061 tagaagagat ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca
     9121 gttcttcttg cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa
     9181 aagcaggagt aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt
     9241 tcgttagttc ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta
     9301 tattggagtg tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat
     9361 ccggattggc agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg
     9421 taatagcacg tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca
     9481 tttatggtca cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga
     9541 tgctgtttca ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg
     9601 caaatccgcc atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc
     9661 aattccaatc aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat
     9721 tcccccagca ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact
     9781 gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc
     9841 ttttgttgtc tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt
     9901 tagccatgtg acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt
     9961 atgtaacagg gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt
    10021 tttatcggag gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc
    10081 tgctgtacca aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca
    10141 gtctcctggt actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa
    10201 accagtagca agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt
    10261 tccggataaa tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct
    10321 cattgccaat gcatctttat aattgacatc agaataggca atttttacca agacttcatt
    10381 ttcagccaac tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat
    10441 tacctgaaaa gatcgaaaag cgttcatttg tgagacactc cttttaaaat tttcttttat
    10501 cataccataa agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt
    10561 gaaaaataaa gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa
    10621 aaaaatttta caaatggttg cctttttcaa taaatttcgt atactaatac gagtggtagt
    10681 aaacaggaag tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga
    10741 agttaagaaa tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag
    10801 atttgtcaaa agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa
    10861 cattcttttc tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag
    10921 cagcgaatct gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa
    10981 acgggtatga attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct
    11041 tgctgacttt tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt
    11101 ttatttttgt attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa
    11161 aagcaggaca gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca
    11221 aaaaacgaac aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa
    11281 aacgtgggga aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca
    11341 gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct
    11401 tccggctgtg gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa
    11461 ggggacatct attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta
    11521 aatacagtct tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct
    11581 tttggattga agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct
    11641 ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt
    11701 caaagacagc gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta
    11761 gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg
    11821 gatctgcaac aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg
    11881 ttggcaatga gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca
    11941 cccgtagata tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc
    12001 aatatcgtgg acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt
    12061 gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag
    12121 gatttgacga tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg
    12181 tttcgtggtg tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc
    12241 cattcaacga aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat
    12301 atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac
    12361 gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt
    12421 tgtggctcgg tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg
    12481 atatgaatgg gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc
    12541 tttctatgac gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca
    12601 tcagttaccc gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc
    12661 tgattatttt gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt
    12721 tcagtgtgca cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga
    12781 tattattcac agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga
    12841 ttatgccaat tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag
    12901 atttaggcgc gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg
    12961 gtgtgaaaag tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc
    13021 gtctgattgg cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg
    13081 ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca
    13141 tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta
    13201 aatggtcgta tctttattta gggatcgtct ttttattctt atatgcaccg atcttctatc
    13261 tgatttttta ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg
    13321 aaaattatga agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca
    13381 tgctggcttt cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa
    13441 tctattacac gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat
    13501 tagtctctcc tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat
    13561 tcggattagg ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg
    13621 tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag
    13681 accttggtgc taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag
    13741 gaatcatagc tggatatttt atggcattta cctattctct tgatgatttt gcggttacat
    13801 ttttcgtcac aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac
    13861 aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat
    13921 tggtcattgg atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca
    13981 gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg
    14041 tattctcatc attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg
    14101 catgagtgga gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt
    14161 gatcacgaaa tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa
    14221 tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag
    14281 tgaatatatg atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa
    14341 gatcaaagga ttaaataata ttgatccacg atttttagac ttggattttg atccagataa
    14401 tcagtattcg attccatatt tttggggaac tttagggatc gtatataacg ataaattctt
    14461 tgatgcaaat caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt
    14521 gatgctgatt gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc
    14581 tttgaatagt aagaatatgg cacagctgac agaagcagca aataaattga atcatttaac
    14641 accaaatgtc aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc
    14701 ctccgtggca gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt
    14761 acattatgtg atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa
    14821 aaccgtgaaa aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa
    14881 tgcggcacaa aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt
    14941 attgccaaaa gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca
    15001 tcttgaagtc tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt
    15061 agaattcaag atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg
    15121 ttctaaagag ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc
    15181 ggatgaaacg ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag
    15241 aaaagtggac aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga
    15301 aaaagttaca gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa
    15361 tctattaata aaactaatat atgaatccag ttcaatattt gtagctatga aaaaacataa
    15421 aaatcatatt agataaaaac taaattggta gataaattat tttgtcagat atctgaggca
    15481 aatattcgac tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat
    15541 tgcagccgat agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga
    15601 gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca
    15661 ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga
    15721 cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc
    15781 ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg
    15841 tagttggtca tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat
    15901 atccgcctaa cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt
    15961 tagccacaaa aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat
    16021 ttgctatctt aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt
    16081 ttggatacaa gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa
    16141 cggccaacaa cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag
    16201 aaaagctctt tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt
    16261 ggcattctgc taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat
    16321 tagacacctt tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc
    16381 ccattcgaga acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac
    16441 aaaaagatat tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg
    16501 ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa
    16561 cacaagaaga accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg
    16621 aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa
    16681 aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga
    16741 taaatgaagg taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg
    16801 tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt
    16861 ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg
    16921 ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg
    16981 ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct
    17041 tggagcattt accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg
    17101 tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg
    17161 gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt
    17221 ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga
    17281 atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt
    17341 taaatcaaat ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata
    17401 atttgacgac ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta
    17461 aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct
    17521 tttctcaaag tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat
    17581 tgaaagatgt ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact
    17641 acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat
    17701 tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg
    17761 acatcttaga cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc
    17821 cggcagaatt taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg
    17881 cggaccgttt gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt
    17941 ctaaatatga aaattactat catttcactt taaatggccg tgtaaaacta aatctctata
    18001 tgcatacggc attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac
    18061 cgaattatga tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc
    18121 aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg
    18181 aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt
    18241 ttaggttaga cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt
    18301 ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa
    18361 attctaatag cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg
    18421 acggggaagg ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa
    18481 caggagttga atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc
    18541 gatttatttg gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt
    18601 gctgtaacta ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc
    18661 aaggaattaa caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag
    18721 atgaccattc ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa
    18781 gaaaaatgat taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga
    18841 cttggacaaa gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca
    18901 acgatattca aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta
    18961 aaacagttga tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga
    19021 tggttttagt ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa
    19081 tcgaattaat gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa
    19141 ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg
    19201 aactttatcc agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct
    19261 ctattttgag tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt
    19321 cgctttggcc gtttttattg gagttgctgg acatgaaatg ttcgggatgt ccatgctgag
    19381 tcgtccgatt gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt
    19441 gattgtcggg gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag
    19501 tacggcggag ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg
    19561 aaacgtggat gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat
    19621 gaatatgatt ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa
    19681 atctggtgat caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc
    19741 gctttatgct cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt
    19801 cttggataat attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc
    19861 ggcagttggg atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt
    19921 tttctttggg gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt
    19981 tggaacgatt attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt
    20041 tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt
    20101 ctttcaatga aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg
    20161 ttttggcgct cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt
    20221 ttcgcctatt ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct
    20281 gcggcgctgg aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc
    20341 atggggattg cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa
    20401 agtatcaacg cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg
    20461 ttctggggag tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga
    20521 aatattttgg caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga
    20581 tactatggcg gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg
    20641 aacgggttaa ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt
    20701 gcaatgacga tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa
    20761 gaactaatga atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg
    20821 gtaacattga gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt
    20881 ggaatggtcg tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca
    20941 gctactaggc tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt
    21001 attcacccaa acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa
    21061 acgaatacag ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta
    21121 atcaaaagac attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc
    21181 gagaaaattt actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa
    21241 ccaattcgtt atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg
    21301 atcatcgtat tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt
    21361 cgacgtttaa ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta
    21421 tgcttgttca acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga
    21481 aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa
    21541 aaggaaataa ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg
    21601 acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga
    21661 aggaaacatt gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag
    21721 ttgtccatct ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac
    21781 agggacaggt cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata
    21841 aggatatgta ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa
    21901 cggttctatg gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta
    21961 tcaatcattc tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac
    22021 atactgggga tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa
    22081 ttattgagga catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt
    22141 ggttatcaga cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga
    22201 aggcctgtat tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc
    22261 gatacaaaaa cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg
    22321 gtccttttgg tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag
    22381 tgaagcacta tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg
    22441 tggatggaaa aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc
    22501 ctttttatca atttttagat aataaagatc atttatatca gaacaccatg gccttttctg
    22561 ctagttcttt caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat
    22621 ccggcgccac ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag
    22681 ctttcgtaac ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt
    22741 ggtggccgta tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca
    22801 aatgcggctg ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga
    22861 aattcaaaaa aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga
    22921 atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt
    22981 caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa
    23041 agggaaaaac gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca
    23101 atttaaaaaa atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa
    23161 agctgcgatg ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct
    23221 gacttctggt gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat
    23281 gtggatgacg attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt
    23341 aagaatttat tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt
    23401 ttgtaatggg tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac
    23461 agggggcttt aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga
    23521 gaatttacat ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga
    23581 cgaattggaa gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa
    23641 attacaggag gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac
    23701 ttaccttgcg cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta
    23761 cattgatggg gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg
    23821 cggggcgtgg gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc
    23881 aacagccttt ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga
    23941 aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct
    24001 ttatcgttgg catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca
    24061 acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc
    24121 ttactggtat tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg
    24181 cttgccgcgt taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat
    24241 ggggcaattg aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg
    24301 attttggaaa gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt
    24361 gattttttta aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca
    24421 attgatattc aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct
    24481 tggcaaaatt tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg
    24541 acaacagatg tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta
    24601 gcaggtgttg catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg
    24661 actttcgtgg aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca
    24721 aaggacaatt tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca
    24781 ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg
    24841 ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt
    24901 tcgccagata gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa
    24961 gttcaggata aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc
    25021 tacattatta cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc
    25081 agaaaagcgg taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca
    25141 aaagtattta accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc
    25201 aaataaaaag gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct
    25261 tctgcgtttc cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg
    25321 cgtacactta aatagtaata tattgtgtta ctttatgtct gtggtaatag ttatagttgc
    25381 ttaccaatgt atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga
    25441 atttttttgg tgatagattt tttcagccgt ataaaataaa tcgtaattct attatagaga
    25501 gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt ttctatacat tttaaatatt
    25561 ttattcctat ttctattgct aaaaaagtag aaattctata tttgccttta actctcgctt
    25621 ctcttgcaaa tgtagt
//
LOCUS       DACDEA010000034        24704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDEA010000034 DACDEA010000000
VERSION     DACDEA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.034"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(278..676)
                     /locus_tag="IV136_001510"
     CDS             complement(278..676)
                     /locus_tag="IV136_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8465509.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(673..1446)
                     /locus_tag="IV136_001511"
     CDS             complement(673..1446)
                     /locus_tag="IV136_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8465510.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1443..2126)
                     /locus_tag="IV136_001512"
     CDS             complement(1443..2126)
                     /locus_tag="IV136_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8465511.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2293..3180)
                     /locus_tag="IV136_001513"
     CDS             complement(2293..3180)
                     /locus_tag="IV136_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465512.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3177..4046)
                     /locus_tag="IV136_001514"
     CDS             complement(3177..4046)
                     /locus_tag="IV136_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465513.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4183..5469)
                     /locus_tag="IV136_001515"
     CDS             complement(4183..5469)
                     /locus_tag="IV136_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8465514.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5742..6320
                     /locus_tag="IV136_001516"
     CDS             5742..6320
                     /locus_tag="IV136_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8465515.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6320..7249
                     /locus_tag="IV136_001517"
     CDS             6320..7249
                     /locus_tag="IV136_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465516.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7246..8982
                     /locus_tag="IV136_001518"
     CDS             7246..8982
                     /locus_tag="IV136_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8465517.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="IV136_001519"
     CDS             complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="IV136_001519"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8465518.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9878..10387)
                     /locus_tag="IV136_001520"
     CDS             complement(9878..10387)
                     /locus_tag="IV136_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8465519.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="IV136_001521"
     CDS             complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="IV136_001521"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8465520.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11688..12779)
                     /locus_tag="IV136_001522"
     CDS             complement(11688..12779)
                     /locus_tag="IV136_001522"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8465521.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="IV136_001523"
     CDS             complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="IV136_001523"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8465522.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="IV136_001524"
     CDS             complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="IV136_001524"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8465523.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="IV136_001525"
     CDS             complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="IV136_001525"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8465524.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16742..17716)
                     /locus_tag="IV136_001526"
     CDS             complement(16742..17716)
                     /locus_tag="IV136_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8465525.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17697..19580)
                     /locus_tag="IV136_001527"
     CDS             complement(17697..19580)
                     /locus_tag="IV136_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8465526.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19598..21823)
                     /locus_tag="IV136_001528"
     CDS             complement(19598..21823)
                     /locus_tag="IV136_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8465527.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21938..22972
                     /locus_tag="IV136_001529"
     CDS             21938..22972
                     /locus_tag="IV136_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8465528.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23039..24052)
                     /locus_tag="IV136_001530"
     CDS             complement(23039..24052)
                     /locus_tag="IV136_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8465529.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24130..24318)
                     /locus_tag="IV136_001531"
     CDS             complement(24130..24318)
                     /locus_tag="IV136_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8465530.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7104 a         5373 c         4420 g         7807 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      181 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      241 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      301 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      361 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      421 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      481 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      541 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      601 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      661 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      721 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      781 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      841 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
      901 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
      961 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1021 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1081 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1141 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1201 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1261 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1321 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1381 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1441 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1501 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1561 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1621 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1681 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1741 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1801 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1861 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     1921 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     1981 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2041 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2101 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2161 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2221 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2281 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2341 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2401 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2461 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2521 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2581 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2641 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2701 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2761 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2821 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     2881 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     2941 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3001 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3061 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3121 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3181 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3241 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3301 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3361 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3421 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3481 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3541 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3601 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3661 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3721 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3781 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3841 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     3901 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     3961 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4021 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4081 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4141 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4201 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4261 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4321 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4381 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4441 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4501 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4561 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4621 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4681 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4741 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4801 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4861 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     4921 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     4981 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5041 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5101 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5161 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5221 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5281 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5341 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5401 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5461 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5521 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5581 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5641 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5701 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5761 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5821 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     5881 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     5941 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6001 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6061 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6121 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6181 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6241 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6301 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6361 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6421 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6481 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6541 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6601 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6661 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6721 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6781 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6841 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     6901 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     6961 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7021 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7081 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7141 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7201 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7261 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7321 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7381 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7441 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7501 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7561 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7621 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7681 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7741 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7801 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7861 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     7921 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     7981 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8041 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8101 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8161 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8221 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8281 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8341 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8401 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8461 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8521 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8581 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8641 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8701 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8761 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8821 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     8881 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     8941 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9001 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9061 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9121 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9181 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9241 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9301 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9361 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9421 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9481 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9541 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag gatttttgta
     9601 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9661 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9721 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9781 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9841 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
     9901 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
     9961 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10021 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10081 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10141 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10201 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10261 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10321 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10381 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10441 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10501 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10561 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10621 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10681 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10741 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10801 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10861 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    10921 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    10981 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11041 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11101 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11161 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11221 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11281 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11341 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11401 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11461 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11521 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11581 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11641 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11701 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11761 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11821 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    11881 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    11941 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12001 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12061 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12121 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12181 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12241 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12301 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12361 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12421 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12481 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12541 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12601 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12661 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12721 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12781 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12841 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    12901 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    12961 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13021 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13081 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13141 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13201 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13261 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13321 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13381 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13441 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13501 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13561 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13621 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13681 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13741 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13801 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13861 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    13921 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    13981 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14041 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14101 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14161 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14221 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14281 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14341 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14401 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14461 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14521 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14581 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14641 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14701 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14761 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14821 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    14881 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    14941 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15001 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15061 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15121 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15181 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15241 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15301 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15361 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15421 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15481 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15541 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15601 cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa
    15661 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15721 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15781 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15841 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    15901 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    15961 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16021 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16081 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16141 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16201 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16261 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16321 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16381 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16441 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16501 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16561 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16621 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16681 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16741 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16801 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16861 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    16921 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    16981 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17041 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17101 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17161 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17221 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17281 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17341 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17401 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17461 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17521 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17581 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17641 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17701 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17761 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17821 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    17881 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    17941 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18001 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18061 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18121 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18181 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18241 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18301 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18361 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18421 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18481 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18541 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18601 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18661 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18721 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18781 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18841 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    18901 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    18961 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19021 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19081 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19141 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19201 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19261 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19321 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
    19381 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19441 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19501 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19561 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19621 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19681 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19741 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19801 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19861 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    19921 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
    19981 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20041 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20101 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20161 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20221 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20281 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20341 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20401 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20461 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20521 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20581 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20641 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20701 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20761 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20821 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    20881 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    20941 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21001 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21061 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21121 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21181 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21241 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21301 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21361 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21421 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21481 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21541 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21601 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21661 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21721 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21781 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21841 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
    21901 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
    21961 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
    22021 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
    22081 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
    22141 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
    22201 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
    22261 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
    22321 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
    22381 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
    22441 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
    22501 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
    22561 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
    22621 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
    22681 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
    22741 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
    22801 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
    22861 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
    22921 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
    22981 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
    23041 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
    23101 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
    23161 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
    23221 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
    23281 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
    23341 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
    23401 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
    23461 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
    23521 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
    23581 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
    23641 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
    23701 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
    23761 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
    23821 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
    23881 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
    23941 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
    24001 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
    24061 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
    24121 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
    24181 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
    24241 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
    24301 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
    24361 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
    24421 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
    24481 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
    24541 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
    24601 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
    24661 agtccttatt ggcaaatcgc caaaataatg tagactaact aaaa
//
LOCUS       DACDEA010000035        24310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDEA010000035 DACDEA010000000
VERSION     DACDEA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.035"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1152)
                     /locus_tag="IV136_001532"
     CDS             complement(<1..1152)
                     /locus_tag="IV136_001532"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8465531.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1337..3778
                     /locus_tag="IV136_001533"
     CDS             1337..3778
                     /locus_tag="IV136_001533"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8465532.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4108..4812)
                     /locus_tag="IV136_001534"
     CDS             complement(4108..4812)
                     /locus_tag="IV136_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465533.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4999..5244)
                     /locus_tag="IV136_001535"
     CDS             complement(4999..5244)
                     /locus_tag="IV136_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465534.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5261..6112)
                     /locus_tag="IV136_001536"
     CDS             complement(5261..6112)
                     /locus_tag="IV136_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8465535.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6278..7282
                     /locus_tag="IV136_001537"
     CDS             6278..7282
                     /locus_tag="IV136_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8465536.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7523..7879)
                     /locus_tag="IV136_001538"
     CDS             complement(7523..7879)
                     /locus_tag="IV136_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8465537.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8247..9434
                     /gene="tuf"
                     /locus_tag="IV136_001539"
     CDS             8247..9434
                     /gene="tuf"
                     /locus_tag="IV136_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8465538.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(9622..12213)
                     /gene="mprF"
                     /locus_tag="IV136_001540"
     CDS             complement(9622..12213)
                     /gene="mprF"
                     /locus_tag="IV136_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8465539.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12239..12958)
                     /locus_tag="IV136_001541"
     CDS             complement(12239..12958)
                     /locus_tag="IV136_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8465540.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13278..13799
                     /locus_tag="IV136_001542"
     CDS             13278..13799
                     /locus_tag="IV136_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8465541.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            13858..14718
                     /locus_tag="IV136_001543"
     CDS             13858..14718
                     /locus_tag="IV136_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8465542.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            14825..17218
                     /locus_tag="IV136_001544"
     CDS             14825..17218
                     /locus_tag="IV136_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8465543.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17400..19241
                     /locus_tag="IV136_001545"
     CDS             17400..19241
                     /locus_tag="IV136_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8465544.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19265..20374
                     /gene="rpoD"
                     /locus_tag="IV136_001546"
     CDS             19265..20374
                     /gene="rpoD"
                     /locus_tag="IV136_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8465545.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            20594..22693
                     /locus_tag="IV136_001547"
     CDS             20594..22693
                     /locus_tag="IV136_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8465546.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            22889..23752
                     /locus_tag="IV136_001548"
     CDS             22889..23752
                     /locus_tag="IV136_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8465547.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            23857..>24310
                     /locus_tag="IV136_001549"
     CDS             23857..>24310
                     /locus_tag="IV136_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8465548.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         8126 a         4299 c         5037 g         6848 t
ORIGIN      
        1 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
       61 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
      121 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
      181 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
      241 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
      301 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
      361 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
      421 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
      481 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
      541 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
      601 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
      661 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
      721 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
      781 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
      841 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
      901 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
      961 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     1021 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     1081 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     1141 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     1201 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     1261 tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     1321 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     1381 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     1441 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa
     1501 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     1561 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     1621 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     1681 agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg
     1741 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     1801 gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg
     1861 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     1921 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     1981 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     2041 tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc
     2101 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     2161 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     2221 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     2281 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     2341 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     2401 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     2461 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     2521 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     2581 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     2641 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca
     2701 gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     2761 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     2821 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     2881 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     2941 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     3001 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     3061 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     3121 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     3181 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
     3241 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
     3301 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
     3361 ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
     3421 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
     3481 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
     3541 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
     3601 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
     3661 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
     3721 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
     3781 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt
     3841 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
     3901 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
     3961 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
     4021 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
     4081 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
     4141 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
     4201 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
     4261 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
     4321 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
     4381 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
     4441 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
     4501 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
     4561 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
     4621 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
     4681 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
     4741 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
     4801 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
     4861 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     4921 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
     4981 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
     5041 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
     5101 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
     5161 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
     5221 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
     5281 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
     5341 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
     5401 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
     5461 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
     5521 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta
     5581 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
     5641 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
     5701 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
     5761 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
     5821 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
     5881 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc
     5941 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
     6001 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
     6061 ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag
     6121 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
     6181 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
     6241 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
     6301 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
     6361 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
     6421 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
     6481 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
     6541 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt
     6601 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
     6661 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
     6721 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
     6781 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
     6841 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
     6901 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
     6961 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
     7021 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
     7081 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
     7141 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
     7201 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
     7261 tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc
     7321 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg
     7381 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
     7441 agaagcaact ccttcttatt tcttggagtt aaacgcttct gtcccagcct ctttttatct
     7501 aataaaattt taagaccatt tactaattat cgattttttc tgccgcattt ttaggcactt
     7561 cagaagcctt tatttcttcc cagcttaata cacctttacg aggattgact ttgagtttaa
     7621 gatacgcatt catctttaat ggatgttctc tttcttcgcg aagcgtcacc tgtttttttt
     7681 caccatcttt attataagcc gcttgcttgt aggtataagt cgtgaatttc tctccgttat
     7741 ctgcttgggt agctgacatt tcgccattcg tcgtgatttt tgtataataa gcttcgccgc
     7801 cgtaaaaata atgataagct gcaaaacttc cgccgagaaa tatgcctaaa acgacaagta
     7861 cgccaattaa ttttttcatg tacttcacct ctttccttat tattaacgaa atagaagatt
     7921 tcgtctattg aaagttgttg gcaacagctg aattttaaac ttttcctcat tttgttctta
     7981 ttgcttcaaa agaaagttaa ggactatctt gaagtttttc tcaaaaaaca cattttttta
     8041 tttataaaag gtactttttt cgcgtataag acagacgagt gctcttagtt gactttgacc
     8101 aatattgacg tataataagc ttagttcgaa tcttacaaaa agtaagtcgg acgtattctt
     8161 tttgatttag tcaaaaaaag actataataa aaaggtgaga gctgaaattt ttcagtgatc
     8221 tatattattt aggaggtttg taaattatgg caaaagaaca ttatgacaga agtaaaccac
     8281 acgttaacat tggtacgatt ggacacgttg accatgggaa aactacttta actgctgcga
     8341 tcactacagt attaggcaaa aaaggcctag caaatcctca agactatgct agtattgatg
     8401 cagctccaga agaacgtgaa cgtgggatta cgatcaatac cgcacacgta gaatatgaaa
     8461 cagaaaaacg ccactacgcg catattgatg caccaggaca cgcggactat gtgaaaaaca
     8521 tgattacagg ggcagcacaa atggatggtg caatcttagt tgtttctgca actgacggtc
     8581 cgatgcctca aacacgtgaa cacattttat tgtcacgcca agttggtgta aaatacctga
     8641 ttgttttctt gaacaaagtt gatttagtcg atgatgaaga attgatcgat ttggtagaaa
     8701 tggaagttcg cgagttattg agcgaatatg gtttcccagg cgatgacact cctgtgatca
     8761 aaggttccgc attaaaagca ttgcaaggcg atccagatgc tgaagctgct attatggaat
     8821 tgatggatac agtagatgaa tatatcccaa caccagaacg tgatacagat aaaccattac
     8881 tattgccagt ggaagacgtc ttctcaatta caggtcgagg aactgttgcc tcaggtcgta
     8941 ttgatcgtgg tgctgttcgt gtcggtgatg aggtagagat cgtagggatc aaacctgaaa
     9001 cacaaaaagc agttgtaaca ggtgtagaaa tgttccgtaa aacgttagat tacggggaag
     9061 ctggggataa cgtaggcgtg ttgttacggg ggatccaacg tgacgatatc gaacgtggac
     9121 aagtacttgc taaaccaggt tccattactc cacatacaaa attcaaagca gaagtgtacg
     9181 tgttgacaaa agaagaaggt ggacgtcata ctccattctt caacaactat cgtccacagt
     9241 tctacttccg cacaactgat gttacaggaa caatcacatt gccagaagat acagaaatgg
     9301 tcatgcctgg tgacaacgtg acgattgatg ttgacttgat ccatccaatc gctgttgaaa
     9361 acggaacgac attctcaatt cgtgaaggcg gacggacagt aggttcaggt atcgttacag
     9421 agatcgaagc ataagaaacg cgactcgaat attttggtaa tgaaaaatgt ttaaaataaa
     9481 agagcggtcc aaacaggatt tctgtttgga ccgctctttg ttcttcaatt gtataagaag
     9541 agattgtaga agcatgaact ttcgttttac ttcagtctct tttattcatc atgagagtgt
     9601 tcatctatcg gtatgcagtt tttaatactt tcttcgtatc cattttttca aaccacttat
     9661 tctaccttgt gtctgctcaa ctggcgcatt atcaataatc agcaaagcaa tcataacata
     9721 tgcaatccaa ctatctctag aatatagagt ataacgtgag acccagtttg tagcgtactt
     9781 gcttttatat tctcttaatc catggaaaga ataaaaatga gaaccaaact cgtaaaccaa
     9841 gtatgcaatc ctttcctgta aaaaactctt tctggaagtt ccgacatttg aaagaggggc
     9901 catccctaaa ttgaaccatt ggatatgttc ttctttcata taggcaaaca aatgcaagaa
     9961 caagaaatcc atactgccag aaggtgcttt tttagaatca tatcgcatca gatcaatcgt
    10021 tcctacttta ttgttcgtgt aagtaggcat gatcgttgca aaagcgacaa tttcttcttg
    10081 ggtatttttg acaactgcaa taggcgctct ttgtagataa tcttcagaga aaaagcctaa
    10141 agaaaaccct ttttcctttc gtgaacccaa ccaattatca gagatatttt tgaaagtact
    10201 catttgctca gtagaaaatg gtggttgtaa gacatcaaat gtaaatcctt ctcgttcgat
    10261 ccgattcaag acagcacgag tgcccttcat ttttttccct gaagttgtaa aactattcag
    10321 gtctacataa gcttcttccc ccatcttaat gaaatcgtag ccgaattcat gcaaaatcat
    10381 aacaatctct tcgcttgttt cgtagaaaac tggtaaatag catagtcgat ctgtttcttc
    10441 gataaaagct tcgattgctt ctggaaaatc ttctttctta ccagaaggat cacccattac
    10501 aatacattta ttattgaagc aagcgaattg gagaagaaca gttggttcac cgtctttttc
    10561 ataggcaaac atccgcttat cttttaagaa gatcaattga ctatcggagt ttcctccata
    10621 agttgtaagg atcttcaggg ccttttcttc attaaaggct tcgccaatct gtttcttttc
    10681 tccttgaagg aaatgaacaa atagaacgat catgaaacta acagcgatga tggccaacaa
    10741 tcccgaaaac caaatctttt cagaaggaaa taagaaaaag gaaataaaat ggtgacgccg
    10801 atgagggaaa tccggcagat tgtatacccc aataacgatg taaagcaagg acagtgcacc
    10861 aataatgatt ccatcgatcg tacgccactc ccatgaatag atcaattgtt cccgaaataa
    10921 ttcgttttta gaagcaagaa tgatcagaag cagaatagac aagaaaacca ccggtgtgaa
    10981 actaaaatca cttaataaaa cataaagtaa agctagaaca atcaagaaga ttgtaggcaa
    11041 atatgctcgt ttcacacgtg ctgcgatccc tctgcccata atgattaaaa gaaatcccaa
    11101 tagtatacta ggaaattgga taataaaatg aaatttcaat gggttcaaac tatgaagcca
    11161 gcgaaactct gtaaaagctt gcgggatagt ggcagaaagt actagcatga ttccagaaaa
    11221 gtataataag acaaccacga ttttatgcgc aatttctgtt gctaattgtt tagggatacc
    11281 agaataacgc tgatcaaaag tggaaccgat atttttgaag aaaaagacga ttccaatcaa
    11341 aaacggaatg atgtagtaga acaggcgata gagcaagatc cacacaacga ctatttctct
    11401 tggcacacca atagcggaca acccgataat catcattaca tcgaaactcc caatttctcc
    11461 tggaatcata gacacgattc ctatcactga agcagcaatg aataatggaa ttgtttgcca
    11521 cagatcaatc cggatttcca ttaaataacc aacagaaagg aaactaaaca ggacaccggt
    11581 ccattccaac aatgaaacta gcaatagctg tccttgagtt ttaagtgagg taccaccaag
    11641 gaatcctttc tttttgaaag tagtaaataa aaagacgaca ggaaaatata gaccgccgcc
    11701 gatcaaccat atccaatatt gcttcaaaaa aggagcaact ggcgtgaaga ctacaagtaa
    11761 aaaagaaagc agactgtaaa tagaaagtcc agccatcaaa aacaagaaaa ctttcgataa
    11821 tgcttggatc acgtttctgc tctctttctt ttgtccatat agttcagagc gaagccccac
    11881 actaacaaac ccgccgaaac cggcaatatt gttgatcgta ttgatcagcc agcttgtctc
    11941 aaacaggtaa cgaggattct gcttttgttc aagcatccga ttcaaaataa tatcataacc
    12001 aaccataggt aacacagaga taagaccaat caatagcatt agtccgcttt tccatacagg
    12061 tatagtggca aacgtttctc ctaattgctg gatagataga gttttaccga tggacatcaa
    12121 ttcaccgaca ataatgacaa tgacagaaat caaaaagatt gttttgaaca gtcctaggtg
    12181 gtttttcagc cattgtatgg tattttttaa caaataaatc accttctatt cctaatagtt
    12241 atgtttcaga aagaaattga agaacttctc gattgaagcg ttcaggatct tttctagcta
    12301 attcatgtcc ttgttctttc acaagcacaa aggaagcttt tggtatagtt ttagctatat
    12361 acaaagaatg ttttagcttg attacatctt tttttcccac gatgatcaaa gtgggggagt
    12421 tgattttttt taaatcattt tctgtaagcc cgatgtcatg cagcaaaagt ttgataacta
    12481 aaagattttt gcgaagactt gggcgaaata aagagagtaa ccatacccat gcataatgaa
    12541 tattactgat gactctggca gaaaaacgaa cgcctttaac aagcgtgttt ccagaattta
    12601 aaatcaatcg atgaactttt ttagggaagc tgctggcaaa taccagtgct agatttgctc
    12661 catcactaaa tccaagaaaa tctgcctgat cgattttttc cagcaacatg atcgtattca
    12721 gatcctcggc cattaactga aaattcaaca tagatgcttc gtttgtcgaa cgaccatgtc
    12781 ctcggctatc tactagatac acggtatagt agcgttcgag tacagggacc tgttctgaaa
    12841 aaaagcggcc gctgccgtca ttgccatgca gtaaaaagag agggaagcct tgaccgctct
    12901 tttcatagta aatttcgctc ccatctggca tttttgcata tttcttttct tttttcacat
    12961 aatcacctac tgtgtaaagt ataacgatta tatcagtcat tgggcaattg tttttactga
    13021 aaaacaacga tcattttcgt aagatgtcaa aaaatacagg aaaattcatt ttttcttaag
    13081 gcgtacacag aaatcaagga agaaatagcc aagaagataa agaaatagta agataaaaaa
    13141 agatgtcaaa ataactaggt gatgaaatga agcgaaaacg attatctatt atccgtattc
    13201 ttttttacat gatcattctg acggttattg gtgcggtgtt gtttttatga ggaagaactt
    13261 atcgttcggt aaaacgagtg gaaagctatc aaatagaaat aacaacagct gcaaaaaatt
    13321 atcatatgga aaactacgag aatttgatca aggcgataat attgaccgaa tcaaaaggaa
    13381 aagggacaga tctcatgcag agctcggaaa gtctgcatgg agaagctgag atcattgata
    13441 atccagctga cagtattgat caaggtgtca aatatttggc ggaaatgata gtagaagcaa
    13501 agaaacaagg atgcgattta tggacagctg tccaagcata taattttggg aaagattata
    13561 ttgcctatgt aaaaaatcat ggcggagaaa acacagtaga attagcagaa gcgtattcta
    13621 aagatgtgct gagcccactt ttaggaaatg aggaagggaa acaataccga tattggcgga
    13681 tccagtcctt gctttacaat ggcggctatc tctatcataa tggaggtaac atgttttatc
    13741 ctgatattgt caaaatgaat gaaaaaataa tagagaccta tgaaaaagtc tttaagtaaa
    13801 atagcaggaa atgttcaatc atgaataaaa ttaaatgaaa gaaggagtgc gcaaatagtg
    13861 gaaaaaaggc aaaaaatgga aaatcaagaa gtggggtaca caaccaaaga ctggatgtta
    13921 cggtttgtga agggaatgtt tatcggttca ggatttatct tgccaggagt cagcggtggt
    13981 gcattagcag ctgtctttgg catgtatgag cggctgattt cttttttagc ccaccttaca
    14041 aaaaatttca aagaaaatgt tttgtttttt ctgcctgttg gtctaggcgg agtgacaggc
    14101 atctttctac tttctttttt agtcagtttt ttattaggtg cgtatgaaac aacgatatta
    14161 tggttttttg tcggatgtat cgctggaact gttcctgcgt tatggaaaga agcagggaaa
    14221 aaaggaagag atacttctga tgtgatcatc atggttgtta cttttgttct ggcttatttg
    14281 tttttattat gcggcgctca attatttgac gggcaagtcg atcagaattt tggaacatgg
    14341 ctgattgccg gcggattgat tggtttgggt atgatcgtcc caggattgag cccttctaat
    14401 tttttggtgt atatgggatt gtataaagcg atggctgacg gatttaaaga agtacgactt
    14461 gaagtgattg tgccgatcgg aattggtggg attctttgtg ttcttggatt gtcaaaaatc
    14521 atggattaca ttttctcaaa ggcatatagc aagctattcc atttcatttt aggtgtagtt
    14581 tttgcttcaa cgattatgat cattcctacc gattacgcgg gattgggagt tgctggggta
    14641 ctagcttgtc ctgttctttt tatagccggt acagcactag gttggtggat gagccgtttg
    14701 gaggaacaat ataagtaaag atagattgat aataaaaaca gcacacgggt gattcaaaca
    14761 aaaaggttgg catctttttt gtaaaatcat tcgtgtgttt ttctataaat tttataaaaa
    14821 caagttgttt ttaatcttgg aacacgctag acttaaacac gtgattattt ttgaaagttg
    14881 gcggaaaatg gagaaaaaga aaatgaatgg acttacacag caagaggtag ttcagaaaat
    14941 ggcttctggc cagcaaaatg actaccagga agatgcatca aaaagtacaa aacaaatatt
    15001 cagcgataat atattgacgt tattcaattt tctgaacttt ggtattggga tttgtctgtt
    15061 atttgtaggt gcgtattcca acatggcgta tttagcaatt attgtcgtca atatcgttat
    15121 tggaatttat caagaaatac atgcaagaaa tcttgtacgg aaattatcga ttgtttccca
    15181 atcagaagta ctagtgatcc gggacggtaa agaacaatcg atttctacta aagagctggt
    15241 attaggagat ttggtaaaaa tcggagcagg agaacagata ccagccgatt tacaggttat
    15301 atctggacgg gcagaagcaa atgaagctct tttgacaggc gaatctgatt tgattgtcaa
    15361 agaaactgga gctgaattgc tttctggaag ctttttgaca agcggttctg tcatggctga
    15421 agttatccga gtaggtgcag ataattatgc tgtccgcttg actagcgagg caaagactca
    15481 caaagctatt cgttctgaac ttgtagattc gattcgcaaa gtatccaaat tcactagttt
    15541 cgtgattatt cctttgggga tcatactatt tttggaagca ctgttcatcc gcgatgcttc
    15601 tatcaaaatt tctgttattg catctgcagc agctttgtta ggcatgcttc caaagggttt
    15661 ggtattgcta ataagtattg cattaacgac aggggtaacc aaactggcaa aaaaacgaat
    15721 ccttgttcaa gacatgtatt caattgaaac gctggcccat gtcgatacat tatgcttgga
    15781 taaaacaggg accattactg aagggaaaat gagtatccag cgggtagaag cactacatcc
    15841 gctgtacgag aaagccatcc cagaaattat ggggacgtat ctggcagaaa gcagtgataa
    15901 caacatcact atgcgggcta tgagagatta ttttgagctt tctaatcggt atgatgtgac
    15961 aaacaaagtc gcattttctt ctgatcgcaa atggggcgcg atggaatttc cagaactggg
    16021 agtcgtgtac gtaggagcgc ctgaacgttt ggtggaagaa gatcggcttc cggacagtct
    16081 aaaaacagcc caagcgaatg gctatcgcgt acttatgttg gggatcgctc caaatcagca
    16141 attgaacgaa gaaaaaccag catttgttga gccggttgct ctgtttgaaa tcgatgatcc
    16201 aatccgaaaa aacgctcaag aaacactagc ttatcttcat gaggaaggtg tggatctgaa
    16261 agtgatttct ggagacaatc cttttactgt ctccaatatc gctagacgtg caggattacc
    16321 gaattatgat gcgtatgtcg atctttctca aattactgaa gaaatggaag tgcgagaagc
    16381 tgctcatcgc tatacggttt ttggacgagt gagtccgcag caaaagaaat tgctggtaaa
    16441 tgaattgaaa gaaagcggca gaacggttgc aatgacaggc gacggtgtga atgatgtttt
    16501 ggcgttaaga gaagcagatt gcagcattgc tatggcagaa ggtgatggag caacacgtca
    16561 gattgcgaat ctggtattgt tggactctga ttttactaca ttgccagaag tcctgtttga
    16621 aggacgccga gtggtcaaca atgtgacgaa agtctcaggc atctttttta tcaaaacgat
    16681 ttattcattt attttatcga ttatatgtgc cgttacagca attggatttc cgtttatccc
    16741 tatccaaatc acgctgattg atttagcgat tgaagggtat ccttccttct ttttatcttt
    16801 tgaacaagat aaaagaaaaa tcacttaccg ttatttgcct acagcactta ttaatgcttt
    16861 accaaatgcc ttgcttgtag tagtgaatat cattgcggtg tatctacttg gaaaaacatt
    16921 cgcatgggga aatgctgaaa ctactacttt gatgtattac ttgctgattg ggatcagttg
    16981 catggcagtt gtaaaagctt gttatccctt caacccattg cgcatcttcc tagcagtaac
    17041 gacgatcgta gggatatatg tagctgcaat gctgttccat ggaattttgg aagtaaactt
    17101 agggttggga gagagcgtag gatacttctt tgtattcatg ctcgtaaata ttgttcttcg
    17161 agtattgtac tggaaggctg atattcaaaa tcagttactg aaaagagtag caaattgatg
    17221 cttaatgaga tagaaatgtg ttatacttga aaaagcaatg aaatatgata caatcaaaca
    17281 tgtataattg tgtcctttaa aatcgcactg atttcagtta gtgcgatttt ttaccggtta
    17341 ttctcctgaa atatcttctg ttaaacccaa aagatatcaa tagagcaggg ggtgaaaaaa
    17401 tggctcaacg gattcctcaa gaggttatcg aaacagtacg cagtcaaacg aatatcgttg
    17461 aggtgattgg acaatatgtc cagttgaaaa agtctggtaa aaattacttg gggctgtgtc
    17521 cttttcatga agaacgtaca ccgtcttttt ctgttgcaga agacaagcag atttttcatt
    17581 gctttggttg tggtaaaggc ggaaacgtgt ttacttttct tcaagaacta gaaggtctaa
    17641 gttttccaga agcagtggta aaagtggcag acttcgaaca gataccgtta gaagatcaat
    17701 ggcgtcagcg tactatagct gtccaaaatc ctgaatctgc tacggggaaa ctgattgctg
    17761 ctcatgaaaa agcagcggaa atctatcatc acatgctatt gcatacaaaa gctggacagc
    17821 cagctttaga atatttgcaa agtcgtgggt tgtctttaga attaattgaa gaattcaaca
    17881 taggttttgc acctaatgag cgttcttttc ttcagcaagt attcaagaat gaatcaattt
    17941 caaatgaatt gttagaacgc tctggattgt ttgtcacgca tgatgatggc cgtatgtctg
    18001 atcgttttta tcaaagagtg atgtttccac ttcgtaattc tcaaggccag acgatcggtt
    18061 tttcaggacg ctggcttgca cctgaaaaag ggcaggaaaa agatcagccc aaatacttga
    18121 atagtccaga aacagaactg ttcaataaaa gacaagtttt atttaatctt gataaagcaa
    18181 gaagcgatat ccggaaaaac ggagaaattt tgttgttcga agggtttatg gatgtgattg
    18241 ctgcatggca ggctggtatc aaaactggat tagcttctat ggggacaagt ctaacagctc
    18301 aacagatcca acaaatggaa aagctgacga aagaattggt cttttgctat gacggagaca
    18361 acgcaggttt tgaagcaacc aatcgaggga ttgaattgct tcggcaaaac agtcgattag
    18421 cactttctgt cgtagtcgtt ccagaaaagc ttgatcctga tgaatatctc cgtaagtatg
    18481 gagaagcatc tttccaagaa ttagtggctc atgggcgcga aacagtcttt acttttaaat
    18541 cacgttattt aaaacaaggg aaaaatttag ataatgaaaa agacaagatc gaatacctcg
    18601 aagaattact agttgaactt agccgagttg tttcaccaat cgaacaagat atgtatctat
    18661 ctcaactgtc aacagagttt cagatcggca gagagacgat acaaaaacaa ctgcgcgatc
    18721 ttcgaaggac aaatcagcaa aatcgtcaag aacctcagga aagtcaacac actcaggtta
    18781 cgagacaaga aactgcacgg aaaaagagac ctttgacgca agtagaaaag gcagagcaga
    18841 ttctattata tcgtgcattt aaagaagttt ctgtccgtaa tattttaaaa gaacgagaat
    18901 ttcagtttat tcatgatgca tatgcagaag tttatttttt attcgatgca tatgtctcac
    18961 aacattatga attcaacctt gcagagtttt tagacttttt gcaagatgaa aatttaaggc
    19021 ggttggtggt ggaaatcgaa tatttaccgg tggcagaaga aagttcgcca cgagaaatcc
    19081 aagacttgct agatgtgatc agcaaatctg gcttagctga tgaaataaca ttaaaaaaac
    19141 aaatgcagca agaagccaga cgaactggaa acaaacagct tgagcttgaa ttgacgattg
    19201 aaatcatcaa tttaacgaaa cagttaaaac aagccaaata gctactcaag gggggccttc
    19261 ttttatggca aatggaacag ataaaaaaac gtatgaagca gcagtagcag ctttcatcaa
    19321 agaaaacaaa cccaaacgac aagttgtata tgatgattta tccaatcagc ttgctacacc
    19381 attcactttg aatgcagatg aaatggacaa attgatccaa aagattgaag acgcaggtat
    19441 cagtgtggtg gatgaaaatg gtgatccatc aattcacagc ttgaagagtg ctgagaaaaa
    19501 agctgaacaa gcaaaaacag aagatctttc tgcgccaacc ggtgtaaaaa tcaatgaccc
    19561 ggttcgtatg tatctgaaag aaattggccg tgtctccctt ttaactgcgg aagaagaagt
    19621 agcgttagca ttgaaaatcg aagaaggcga ccaagaagcg aaacagcgtc tggcagaagc
    19681 aaaccttcgt ttagtagtaa gtatcgctaa acgttatgta ggacgaggca tgcagttcct
    19741 tgacttgatc caagaaggaa acatgggact gatgaaggca gttgaaaaat ttgactaccg
    19801 caaaggattt aagttttcaa cctatgctac atggtggatt cgtcaagcca tcactcgagc
    19861 aatcgctgac caagcacgta cgatccgtat tccagttcat atggttgaaa caattaataa
    19921 attgatacgt atccaacgtc aattgctgca agaccttgga agagaaccta cgccagaaga
    19981 aatcggtgcg gaaatggatc ttccaacaga aaaagtccga gaaatcttga aaatcgcgca
    20041 agaacctgtt tcccttgaaa cgccaatcgg tgaagaagat gactctcact taggtgattt
    20101 catagaagac caggaagcaa caagtccagc agaacacgca gcttatgagt tactaaaaga
    20161 acaattggaa gatgtactag atacattgac tgatcgggaa gagaatgtcc ttcgcttacg
    20221 ttttggttta gatgatggcc ggacgagaac attggaagaa gtagggaaag tatttggtgt
    20281 tactcgggaa cgtattcgtc agatcgaggc taaagcatta agaaaattgc gccatccttc
    20341 tcgttcaaaa caattaaaag atttcttaga ataagcgtgg actattgacc agaagagaaa
    20401 tcagtaaaac tgtttggttt attttaaaag aaaaaaagct tgagactacg cagtctcaag
    20461 cttttttttc tataggaagg aaattcattc attattttta agtaaatcct aaaaataagc
    20521 tattggcgtg acgtatagaa tatgatagat taaatggtag cgtaaaccta gtaaaggagc
    20581 cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg aaaaaaatgg agcagaatac
    20641 tatctgtcca gactgcggtc atcaaataac tgcagaagat cagcaaataa aagaatcaat
    20701 tgaagaaaca aatacagatg cagctatgca ggataaatca actgaagatg cagtctttcc
    20761 tgatacagaa ttaaacgatc caatcgaatg gtcggaattg aaagatttac ctctagaatc
    20821 tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa gaaccacctg ctcacgatca
    20881 agtaactaat atagagaaaa atgaacagaa gaaagcaact gttcatgaga cttcggaaga
    20941 atcaggaaag aatcaagaag aaacgcttga atcaaaacaa aaaagaagag tagctgaact
    21001 gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta tccgcttata tcaaagcaca
    21061 tcgtgaagat acaaaagaag agcacgcgaa agagctgctc aaaatgatca gtgaaaaaat
    21121 tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa actgaaaaag actctgatag
    21181 agaaaacgcc acaggtaaag aaacagtaaa aataaaagac gtaaaaacgg atatcacaga
    21241 aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa aaaacgaata aactgccgga
    21301 agcagagaaa aaagaaatac tcgaaaacac agacaaaaag tcaacagagc ccgatgaaat
    21361 aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa gagggtactg atcttgaagc
    21421 aaaaatagaa aaagaagaga accagcctaa gctgtttgaa gcagaagcga aatcaataac
    21481 aatcgtgcca gatactgaaa aaacacatca agaatcaaac ttagaaaaaa cagatccaga
    21541 taaagcgcaa aagaaaaaat caaaaaaagt accttatctc gttttagcag cagtacttct
    21601 actcggtgga ggaagctggg cttattatga ccatcatcaa aaagtagaag ccgaagtagc
    21661 agcagaagca aaaagacagc aacaaatgga agaacttcat gagaaacttg ctgcctttta
    21721 tacagatgat caccatcaat ttattcgtgc agatatgatt catcaagatt tatcaaaact
    21781 aaaaaatgat ttatccagtc ttaagaatga aaaaggatat tcagagttag aaacaactta
    21841 ccaagatatc caatctaaga tccgagatat ccaaaaagtg aatgagtggt tcatagcacc
    21901 tgccatcgtc gatgatcatc tagcagatga catcaagttg aaagcagata aagagatcca
    21961 agcaatcgaa acggataaca cagattttgg aaaattgatt caacaagcac aaacagaagc
    22021 taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa acagctatag tctttaaaga
    22081 cggaaaagta gtggattcag caacacgcga ccaatacaag acggcaaaag aagcagtaga
    22141 aaaagtgaaa aatacagatc tatctgctca gttgaatgaa caattgaaac aagtagataa
    22201 gatgctcact gataaagaga aaaaagaaga agaggaaaag aaaaaagcag aagaagcgaa
    22261 aaaagcagct gaagaacaag caaaacaagc cgctcaagca gcagctgcag ctcaagccgc
    22321 tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg gcatcctcga ccccttcctc
    22381 ttcaaactca gcgaatcagc caatcaaatc tgccagagca agtgatattg ccgatgcatc
    22441 taatccagca tggacgtggg caccaggtgt ccaaagcaaa gtgattgcta catgtatcca
    22501 acgtggatat atcgttgaag gaggttacag attagaaaaa gccagaatcg aaaatgggga
    22561 aggctattat aatctatatg ccacttcaac aaaatctgca ttgatgaaag gcattggaga
    22621 aagtgctttg ccattttaca tcgtaaccat caactgtaaa actggatggt tcggaggaaa
    22681 tgggagcaat taattttagg caaatgaaag aggctgtgag cctgccgttt aacaccgttt
    22741 attcgaaaaa ggggagtatg acacaactgt tgtcatactc cttttttcac ctttccaaac
    22801 caaggctcag ctattcaagt agataacatt atgttatgat agcaaagaaa tgtattaaga
    22861 aaacctttca ctatagaggg agaaaaaaat gaacgattta ttagcaacta tatttacagg
    22921 tattattatt gatgaaaatg aaaaaaacta ctttgtccaa aaaaacggac aaacctttgg
    22981 cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca gttgaaggat tcggttacca
    23041 aaatcaaaaa catgaaaatc ggatcactac acaaattcct aaaagccgta tcggtcatta
    23101 cgcttttgga atcgttactg ctacacgaaa agatttaggt gcgtttgtcg atatcggtct
    23161 tccagataaa gactttgtcg tatctcttga tgaattgccg acaatgacag agctatggcc
    23221 taaaaaaggg gatcgcttga tgatttccct tagagttgat gcaaaagacc gtatttgggg
    23281 cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa tatggaacag aagaaatgaa
    23341 aaacaaagat gtgataggaa cagcataccg cttgaaatta acaggtacgt acgttttaac
    23401 ggaagatttc tatcttgggt ttatccatcc ttctgaacga tataatgaac ctcgtctagg
    23461 cgagcagata aaaggtcgag tgattggtgt ccgaccagac ggtgtactga atctttcatt
    23521 gaagccaaga gcatatgaag ctatatcaga tgatgcacaa atgattttaa ccattttaga
    23581 acgttctgca gatcagcaaa tcaactttac agataaatcg gatccagaag aaatcatgaa
    23641 agcttttggt atcagcaaag gagcatttaa gcgcgcatta ggtaatcttt tgaaacaagg
    23701 attgattaca caagaaaacg gtgtgacaaa actagtcaaa aaatctaatt gagaatccct
    23761 tgcatgtttt tcttaactag cttataataa ttttaattaa catagtgtta tatctttttt
    23821 tatttgtaat tattataaat tgttggggtt gagtaaatgg tcatgaatgc tatttcggcg
    23881 atgaaaaaaa caaaacagca gctacatgaa gcggggttta agctgacacc gcaaagagaa
    23941 gctactctgc ttgtgctttt ggaaaatgag aaagagcatt tatctgcaga agaaatcttc
    24001 tttttagtga aaaaaaagag tccggaaatt ggacttgcta ctgtttatcg aacccttgaa
    24061 atcttgacag aattgaaaat tgtcgataaa gtaagtttta atgatggatt agcccgttat
    24121 gatttgagaa aagaaggcgc caaacatttc catcatcatt tactatgctt gaactgcggc
    24181 acgatcgagg aagtagaaga ggatctttta cttgaagtgg agcaagtagt agaagaacgt
    24241 tatcattttt tagtaaaaga tcatcggcta acgttccatg gaatctgtca ggaatgctat
    24301 gaaaaagaaa
//
LOCUS       DACDEA010000036        24073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDEA010000036 DACDEA010000000
VERSION     DACDEA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.036"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(57..602)
                     /locus_tag="IV136_001550"
     CDS             complement(57..602)
                     /locus_tag="IV136_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8465549.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(614..1306)
                     /locus_tag="IV136_001551"
     CDS             complement(614..1306)
                     /locus_tag="IV136_001551"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8465550.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1325..1609)
                     /locus_tag="IV136_001552"
     CDS             complement(1325..1609)
                     /locus_tag="IV136_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465551.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1622..2185)
                     /locus_tag="IV136_001553"
     CDS             complement(1622..2185)
                     /locus_tag="IV136_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8465552.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2413..3075
                     /locus_tag="IV136_001554"
     CDS             2413..3075
                     /locus_tag="IV136_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8465553.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3199..4395
                     /locus_tag="IV136_001555"
     CDS             3199..4395
                     /locus_tag="IV136_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8465554.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4496..5872)
                     /locus_tag="IV136_001556"
     CDS             complement(4496..5872)
                     /locus_tag="IV136_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8465555.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5909..6775)
                     /locus_tag="IV136_001557"
     CDS             complement(5909..6775)
                     /locus_tag="IV136_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8465556.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6864..7889)
                     /locus_tag="IV136_001558"
     CDS             complement(6864..7889)
                     /locus_tag="IV136_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8465557.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7907..8581)
                     /locus_tag="IV136_001559"
     CDS             complement(7907..8581)
                     /locus_tag="IV136_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465558.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8765..9658)
                     /locus_tag="IV136_001560"
     CDS             complement(8765..9658)
                     /locus_tag="IV136_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8465559.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9732..11429)
                     /locus_tag="IV136_001561"
     CDS             complement(9732..11429)
                     /locus_tag="IV136_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8465560.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11422..12735)
                     /locus_tag="IV136_001562"
     CDS             complement(11422..12735)
                     /locus_tag="IV136_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8465561.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12735..16253)
                     /locus_tag="IV136_001563"
     CDS             complement(12735..16253)
                     /locus_tag="IV136_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8465562.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16546..17004)
                     /locus_tag="IV136_001564"
     CDS             complement(16546..17004)
                     /locus_tag="IV136_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8465563.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17301..17819)
                     /locus_tag="IV136_001565"
     CDS             complement(17301..17819)
                     /locus_tag="IV136_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8465564.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17821..18438)
                     /locus_tag="IV136_001566"
     CDS             complement(17821..18438)
                     /locus_tag="IV136_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8465565.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18435..19289)
                     /locus_tag="IV136_001567"
     CDS             complement(18435..19289)
                     /locus_tag="IV136_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465566.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19286..20197)
                     /locus_tag="IV136_001568"
     CDS             complement(19286..20197)
                     /locus_tag="IV136_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465567.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20238..21572)
                     /locus_tag="IV136_001569"
     CDS             complement(20238..21572)
                     /locus_tag="IV136_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465568.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21780..22610
                     /locus_tag="IV136_001570"
     CDS             21780..22610
                     /locus_tag="IV136_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8465569.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22854..23103)
                     /locus_tag="IV136_001571"
                     /pseudo
     CDS             complement(22854..23103)
                     /locus_tag="IV136_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23294..23488)
                     /locus_tag="IV136_001572"
     CDS             complement(23294..23488)
                     /locus_tag="IV136_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465570.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7102 a         5234 c         4150 g         7587 t
ORIGIN      
        1 ttatgctctt cttttattat tttgtgcttt ttattttatc aaatcacttt gtttttttat
       61 aaaatttctg cgcctagtgt ggactcaaaa tgtctcaatg cccattcatg tccaacggga
      121 tcgaaacttg ccacggcatc tttgaaaata tggagtttgt atcctagatt atacgcatcg
      181 actgctgtat gcaacacaca gatatctgta catacgccgg tcaaataaat atctgtgatc
      241 tggcgttctc gcaaacggat gtctagatct gttccactaa aagcggaata atgccgtttg
      301 tctatccaat aaacattttc ctcttgctca tagtcttggt ataaactttt taaagagccg
      361 tacaattccc gtccacccgt tcctatcaca ttatgcggag gaaataagcg gttctccgga
      421 tgaaaggaat cttttggatc atgcgcatca atggcaaaaa cgacaaaatc cttttgatca
      481 ataaacttct ttgtatgagc agtcagcttt ttttcaatta cttgtcctgc tgtcccagta
      541 gtcagctttc catcctctgc aacaaaatca tatgtgtagt caatcgagat caatgcgttc
      601 atcttagtcc tccttatacg agatgtttga caaattcgat caccatttct gctgatcgtt
      661 tgcctgcgtc ttcgataaat tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc
      721 tcattgcacg tacgatcaaa aatgggatat tgaactgacg agctgtttgt ccaacagcgg
      781 ctccttccat ttcgcaagct aatgcttcag ggaaattcgt taagatttct ttgattttat
      841 ctggcgagtc gacaaatgta tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg
      901 ttgcttttgc tgctttaacc atttcactgc gcaaatatgt gcttgcttca taatatagag
      961 gcattcctgg taattgtcct gggttgtagc caaatcctgt tgcatctgca tcaaaataag
     1021 ctactttgtc tgatataacg atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg
     1081 aacctgtatt gatcaccatg ttcacgccgt attgttggat caacagactt gtggtaatag
     1141 atgctaagac ttttcctata ccggaacgta cgacgatcac ttcgtggtta cctaatgaac
     1201 ctgaaataaa taaagcgcct gcgcgttccc atgataatgg ttcgcttagg ttctctctca
     1261 aaatttttac ttcttcttcc attgcgccga taataccgat tttcatcttt ttaattcctt
     1321 ctttctatat aaatgcgatt gctaaaaaaa ccacacataa cattaggata acaataataa
     1381 gagatttcat caaaaaagag ttccatttgg tcgtcttttc ccgttcgctg atccttgttt
     1441 tcgtgattgg tttattcttt ttgctttctt tacgataaaa gctggcaatc tttttttctt
     1501 cttgctgata agctgtttct gcttttcgct gtttttttaa tgactcgctt gcttgtgctt
     1561 gttgtcgctt gcgaagttcg cttcgcgtaa taagcggtcc tttggccata agccgatccc
     1621 tctactttcc tttggtcaat agttcccagt attgaatcgc ataaatcgtc tttgcatcac
     1681 aaatcaactg atctttcatc gcttgttgcg cctcttcaag agttagatga tacaactcca
     1741 gtacttcgtc ttcatcttgt gctaaaggat tctcgacctt ctctacccct tgtgcatgat
     1801 aaatatgaag gacttcattt gcaaaacctg gtgaaagata catcgaagta agatggctca
     1861 gcgacttcgc acgatagcca gtttcttctt ctaattctct agctgctgtc atttcgggat
     1921 tttgtccttc tcctggatcg atttttccag ctggaatctc caaaatcact ttttctaaag
     1981 gttttcgaaa ttgtttgaca agaagcagac gatcttgttc gtcaaaagca atgatcccga
     2041 caccgccagg atgaaatacc agttctctct tcgaagttcc tccatctggc aaacgaactt
     2101 cgtctacagc aacatcaata atttttccgc tatagatttc tttgcgactg attgtttttt
     2161 cttcaaattg ctcattgttc agcatatctt ctcctgcttt ctttgtaatc ataactgctt
     2221 ttttttcatt tttcaatttt tctcaaaaac tccttattag tttaccatag ataaacctta
     2281 gattttctct aaatatagaa aatccttctt tagtccgata ctctttatag tagtccgtat
     2341 gctaaaataa gtatgtaatc gaataacgaa aagaattcaa tactcaacga taaatgacat
     2401 ggagggttta ttatgataaa acgaaattgg ccgtggcttc tagcattggt cttgctattt
     2461 ctctttgtcc gaaacaatag tttttctttt gttctaggac ttttattgat aggtgtagtt
     2521 ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct
     2581 gcactgactg acgaacttga aactcactat gaaaaaagcg gtatgacagc gaatgaaatt
     2641 tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat
     2701 atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt caaagcagcg
     2761 aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt
     2821 ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga gatcaatgac
     2881 catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca gatcatcgac
     2941 cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga tctagatgat
     3001 atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa atatgaacca
     3061 gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca gatgatgtct
     3121 ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga gaacagattc
     3181 aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt tacaccagtt
     3241 aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga tacgctgacc
     3301 acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc tcgtttaatt
     3361 gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct
     3421 aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt aagtgaattc
     3481 tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg agattcctta
     3541 actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg
     3601 aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa
     3661 tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa agaaaaagac
     3721 ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa agactacttt
     3781 gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact tcaaacaacg
     3841 atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga tgttcagatt
     3901 gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga tcttcgctta
     3961 gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa cacaaatcag
     4021 gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg gaaaaaccaa
     4081 gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca acgccaagtc
     4141 tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat ctcagccatc
     4201 gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca aaccactcaa
     4261 aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa
     4321 cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca aaaactgttg
     4381 gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa caacagttta
     4441 ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
     4501 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
     4561 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
     4621 tcaagatgga gacgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
     4681 gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc aaataggcga
     4741 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
     4801 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
     4861 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
     4921 aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca gcgatcaccg
     4981 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
     5041 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
     5101 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
     5161 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
     5221 ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt ttaacgaaaa
     5281 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
     5341 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
     5401 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
     5461 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
     5521 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
     5581 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
     5641 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
     5701 aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
     5761 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
     5821 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
     5881 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
     5941 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
     6001 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
     6061 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
     6121 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
     6181 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
     6241 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
     6301 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
     6361 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
     6421 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
     6481 tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc atgtacttca
     6541 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
     6601 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
     6661 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
     6721 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
     6781 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
     6841 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
     6901 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
     6961 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
     7021 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
     7081 ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc tatgctcaca
     7141 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
     7201 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga
     7261 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
     7321 agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa
     7381 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
     7441 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
     7501 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
     7561 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
     7621 tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg tatgtcggct
     7681 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
     7741 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
     7801 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
     7861 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
     7921 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
     7981 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
     8041 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
     8101 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
     8161 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
     8221 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaatgtt
     8281 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
     8341 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
     8401 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
     8461 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
     8521 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
     8581 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
     8641 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
     8701 tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct taataattgg
     8761 tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga taacaccaat
     8821 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
     8881 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgtcttc
     8941 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
     9001 gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
     9061 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
     9121 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
     9181 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
     9241 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
     9301 tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt tggacatttc
     9361 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
     9421 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
     9481 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
     9541 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
     9601 ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc tttttcattt
     9661 tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt gctgcagctt
     9721 atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct gtacgctcca
     9781 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
     9841 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
     9901 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
     9961 gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt tccataatct
    10021 aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc ttgtccgctt
    10081 gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga ctccccttta
    10141 taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc atctttaagc
    10201 aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt gactttctct
    10261 ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc aataaccgct
    10321 gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc
    10381 gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt ggctactcca
    10441 ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac tatataatct
    10501 tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc ataataagtt
    10561 agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct tccgttttca
    10621 aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc cgtttttcct
    10681 ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat aatcgttact
    10741 tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat ttcgtttttc
    10801 ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc ccacacgcca
    10861 tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc gattctggaa
    10921 atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga agaaggtgct
    10981 tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt tgtttgtttg
    11041 tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt ttgaatccat
    11101 tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg attatctcga
    11161 atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc tggaatattt
    11221 tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg aacagatgag
    11281 aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac ttcttcggct
    11341 ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa cagtacactc
    11401 attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt ttattaataa
    11461 aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg cttcattcgt
    11521 ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac tcggctcttt
    11581 cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg cggattcata
    11641 ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt taactactac
    11701 atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct cgattctctt
    11761 acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat aataggtgcc
    11821 agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa tgaatttgct
    11881 tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat ttttcaagtc
    11941 attagcagga gccatatcta aataatattt ccccccttct atcatctgat atagagcaaa
    12001 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
    12061 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
    12121 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
    12181 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
    12241 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
    12301 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
    12361 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
    12421 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
    12481 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
    12541 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
    12601 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
    12661 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
    12721 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
    12781 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
    12841 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
    12901 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
    12961 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
    13021 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
    13081 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
    13141 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
    13201 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
    13261 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
    13321 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
    13381 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
    13441 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
    13501 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
    13561 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
    13621 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
    13681 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
    13741 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
    13801 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
    13861 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
    13921 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
    13981 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
    14041 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
    14101 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
    14161 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
    14221 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
    14281 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
    14341 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
    14401 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    14461 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    14521 actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    14581 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    14641 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    14701 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    14761 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    14821 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    14881 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    14941 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    15001 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    15061 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    15121 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    15181 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    15241 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    15301 ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca atgatgataa
    15361 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    15421 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    15481 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    15541 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    15601 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    15661 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    15721 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    15781 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttaat
    15841 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    15901 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    15961 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    16021 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    16081 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    16141 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    16201 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    16261 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    16321 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    16381 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    16441 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    16501 gtaaacttca tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16561 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16621 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16681 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    16741 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    16801 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    16861 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    16921 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    16981 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17041 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17101 tcaaatgata tcctttttag ttaaaaacac caattataca acatttatta ttaaaattaa
    17161 attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata
    17221 aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat
    17281 gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct
    17341 aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa
    17401 gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac
    17461 gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt
    17521 ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt
    17581 tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga
    17641 ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa
    17701 ccaccattag agatattact gataaatttc aaaaaattga tttttcgact atccgttttc
    17761 cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg
    17821 ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa
    17881 ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc
    17941 gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa
    18001 caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta
    18061 actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc
    18121 aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac
    18181 acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa
    18241 tagtgaaccg agctttttct cttcattttt tttgtaatga taaatcatat acagaactgt
    18301 tcgacttgca gttcctaatg tgaaaacagg caagacgcac aggaaccata gtagctgtaa
    18361 atggataaga cgtggaatcc attcaatgat ttctaagaaa taatcattgg cttttttacc
    18421 aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc aatgaaataa
    18481 cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga caagtgttgc tcctgccatc
    18541 agataattcc attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat ctgtaacgtg
    18601 tatttttctt gatcttggat atataacaga ggacctaaga aatcattcca agctgcgttg
    18661 aaggaattga tcgcaatggt aatcaaggca ggtttcgtca atggaagcat caaatgactc
    18721 caaatataca aatgattcgc tccgtcgatt tcagcagctt cgatcaattc attattgatc
    18781 gataagtaaa attgacgcat cagaaatata ttgaacgcat tgccgaagaa cgatggaacg
    18841 atcaggggca aataagtacc tacccagccg atcttggaga ataatacata ttgagggatc
    18901 atcgttacaa accccgggat catcatcgta gaaagaacca aagcaaacat cagctttttc
    18961 ccaggaaaat caagcttcgc aaatccataa gcaataaaag aatttgaaag gacattaccg
    19021 atcactacta gcacagtgat gaataatgta tttcttgcat agcgccaaaa aggaaaggcg
    19081 gcaatcgtat ccagatagtt ttcccatacg attttttctg ggaaaaaggt cggaggataa
    19141 cgaacgattt cttccattgg tttcaatgaa gtaaacacca tccaaagcaa tggaatgatc
    19201 aaaatgattc ctattactgt catgaggaca taacttagta ttttttttgt ttgccgattc
    19261 acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact
    19321 taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg
    19381 acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat
    19441 tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt
    19501 cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag
    19561 tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg
    19621 ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc
    19681 ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca
    19741 gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg
    19801 gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat
    19861 agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa
    19921 acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata
    19981 acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga
    20041 aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag
    20101 gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc
    20161 ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct
    20221 agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca
    20281 tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc
    20341 actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag
    20401 ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact
    20461 aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct
    20521 tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct
    20581 ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct
    20641 ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta
    20701 gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat
    20761 acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc
    20821 cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca
    20881 tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa
    20941 ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta
    21001 cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct
    21061 tctttaaata actttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca
    21121 taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg
    21181 tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg
    21241 gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat
    21301 ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat
    21361 tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag
    21421 gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt
    21481 gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt
    21541 gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg
    21601 atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga
    21661 ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa
    21721 ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga
    21781 tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa
    21841 tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc
    21901 agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg
    21961 acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat
    22021 tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg
    22081 ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga cctcgtcctg
    22141 ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag
    22201 cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt
    22261 acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata
    22321 gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa
    22381 tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct
    22441 ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg
    22501 atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat
    22561 tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc
    22621 gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa
    22681 tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca
    22741 gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt
    22801 gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct
    22861 cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg
    22921 atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga
    22981 tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg
    23041 atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca
    23101 catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt
    23161 tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc
    23221 gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt
    23281 acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa
    23341 gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag
    23401 aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt
    23461 tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc
    23521 acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg
    23581 actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc
    23641 taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc
    23701 agtatacata accttgatac ttcgaccact tatatatcga acacacaaat acctatccat
    23761 tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg
    23821 acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac
    23881 tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc
    23941 gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct
    24001 ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat
    24061 cattaataaa tgt
//
LOCUS       DACDEA010000037        23080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDEA010000037 DACDEA010000000
VERSION     DACDEA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.037"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IV136_001573"
     CDS             complement(<1..357)
                     /locus_tag="IV136_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465571.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYI"
     gene            complement(364..912)
                     /locus_tag="IV136_001574"
     CDS             complement(364..912)
                     /locus_tag="IV136_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8465572.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(957..1289)
                     /locus_tag="IV136_001575"
     CDS             complement(957..1289)
                     /locus_tag="IV136_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465573.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1303..1533)
                     /locus_tag="IV136_001576"
     CDS             complement(1303..1533)
                     /locus_tag="IV136_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465574.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1755..2297)
                     /locus_tag="IV136_001577"
     CDS             complement(1755..2297)
                     /locus_tag="IV136_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465575.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2318..3409)
                     /locus_tag="IV136_001578"
     CDS             complement(2318..3409)
                     /locus_tag="IV136_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8465576.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3417..5627)
                     /locus_tag="IV136_001579"
     CDS             complement(3417..5627)
                     /locus_tag="IV136_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465577.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5643..8171)
                     /locus_tag="IV136_001580"
     CDS             complement(5643..8171)
                     /locus_tag="IV136_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8465578.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8223..8633)
                     /locus_tag="IV136_001581"
     CDS             complement(8223..8633)
                     /locus_tag="IV136_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465579.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8646..8864)
                     /locus_tag="IV136_001582"
     CDS             complement(8646..8864)
                     /locus_tag="IV136_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465580.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8861..9853)
                     /locus_tag="IV136_001583"
     CDS             complement(8861..9853)
                     /locus_tag="IV136_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465581.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9932..10843)
                     /locus_tag="IV136_001584"
     CDS             complement(9932..10843)
                     /locus_tag="IV136_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8465582.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10862..11206)
                     /locus_tag="IV136_001585"
     CDS             complement(10862..11206)
                     /locus_tag="IV136_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465583.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11226..11782)
                     /locus_tag="IV136_001586"
                     /pseudo
     CDS             complement(11226..11782)
                     /locus_tag="IV136_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11863..12363)
                     /locus_tag="IV136_001587"
     CDS             complement(11863..12363)
                     /locus_tag="IV136_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465584.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12364..12909)
                     /locus_tag="IV136_001588"
     CDS             complement(12364..12909)
                     /locus_tag="IV136_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465585.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12906..14162)
                     /locus_tag="IV136_001589"
     CDS             complement(12906..14162)
                     /locus_tag="IV136_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8465586.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14523..14834)
                     /locus_tag="IV136_001590"
     CDS             complement(14523..14834)
                     /locus_tag="IV136_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465587.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14857..16167)
                     /locus_tag="IV136_001591"
     CDS             complement(14857..16167)
                     /locus_tag="IV136_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8465588.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16781..17233)
                     /locus_tag="IV136_001592"
     CDS             complement(16781..17233)
                     /locus_tag="IV136_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465589.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17235..17600)
                     /locus_tag="IV136_001593"
     CDS             complement(17235..17600)
                     /locus_tag="IV136_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465590.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17699..21982)
                     /locus_tag="IV136_001594"
     CDS             complement(17699..21982)
                     /locus_tag="IV136_001594"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8465591.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22129..22368)
                     /locus_tag="IV136_001595"
     CDS             complement(22129..22368)
                     /locus_tag="IV136_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465592.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22458..22730)
                     /locus_tag="IV136_001596"
     CDS             complement(22458..22730)
                     /locus_tag="IV136_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465593.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(22813..23064)
                     /locus_tag="IV136_001597"
     CDS             complement(22813..23064)
                     /locus_tag="IV136_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465594.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6787 a         4985 c         3396 g         7912 t
ORIGIN      
        1 aatatagaaa tacattcttt tccagttaca tttaattttt aaacaaaatt ctccccaaaa
       61 aggaactttt tctagtgaaa acttggtaat ctctttgtaa ttaattcttg ttaaagaata
      121 attaaacact tcatttttac ttgttatttt ttcattgact ataactagtt catctggtaa
      181 aaaaattaac caacaaacat cgacaaacag taaatgatat aatccatttc cacctccaag
      241 catattcaat actggtgaac atgccttaga aacacgaata tagttatggt ttaaatagat
      301 attttcttta aacattgctt gtttaaaagc catatgctct tgttctaaac ttttcataaa
      361 cttttactct aatgcacagc tagcagcagc tgctgcaatt cctgctacaa tatttacctt
      421 agctaactct ttagctgcag cttttcctaa ttgtttttct gcaattttta ataataaatc
      481 cgcagctttt tcaaaattaa aagcttttaa tgcttcaata aaatctccct gcgtacctat
      541 agcccaagcc actcctcctc caggaagaga attaatggct acacatccag catatcgttt
      601 ccaatcaact ttctccattc taggaacaat aatatgttca taaaagactc ttgctgctct
      661 tccttgttcg ttatcttgac tgattcgttc acgaatagca ccaaaatcct ttatatggta
      721 tacaccatgt tcgtctaaaa aaccaactgt atcaaaataa aaagttaatt catctactaa
      781 tttattcttt atttctaaat caactgtatt cactttttct gtggcataca cacttgttcc
      841 agtactaaca aaagtactta aacctaataa gaaaacacct aaaacaccaa atattttaat
      901 tattcttttc ataatttctc ctccataaaa aataagttac tttttaaata tcttgtttat
      961 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
     1021 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
     1081 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
     1141 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
     1201 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
     1261 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
     1321 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
     1381 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
     1441 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
     1501 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
     1561 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
     1621 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
     1681 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
     1741 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
     1801 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
     1861 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
     1921 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1981 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     2041 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     2101 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     2161 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     2221 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     2281 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     2341 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     2401 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     2461 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     2521 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     2581 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     2641 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     2701 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     2761 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     2821 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     2881 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     2941 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     3001 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     3061 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     3121 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     3181 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     3241 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     3301 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     3361 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     3421 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     3481 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     3541 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     3601 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     3661 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     3721 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     3781 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     3841 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     3901 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3961 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     4021 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     4081 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     4141 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     4201 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     4261 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     4321 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     4381 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     4441 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     4501 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     4561 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     4621 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     4681 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     4741 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     4801 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     4861 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     4921 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4981 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     5041 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     5101 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     5161 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     5221 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     5281 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     5341 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     5401 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     5461 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     5521 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     5581 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     5641 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     5701 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     5761 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     5821 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     5881 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     5941 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     6001 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     6061 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     6121 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     6181 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     6241 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     6301 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     6361 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     6421 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     6481 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     6541 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     6601 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     6661 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     6721 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     6781 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     6841 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     6901 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6961 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     7021 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     7081 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     7141 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     7201 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     7261 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     7321 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     7381 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     7441 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     7501 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     7561 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     7621 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     7681 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     7741 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     7801 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     7861 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     7921 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7981 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     8041 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     8101 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     8161 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     8221 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     8281 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     8341 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     8401 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     8461 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     8521 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     8581 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     8641 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     8701 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     8761 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     8821 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     8881 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     8941 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     9001 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     9061 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     9121 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     9181 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     9241 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     9301 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     9361 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     9421 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     9481 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     9541 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     9601 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     9661 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     9721 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     9781 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     9841 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     9901 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9961 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
    10021 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
    10081 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
    10141 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
    10201 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
    10261 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
    10321 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
    10381 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
    10441 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
    10501 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
    10561 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
    10621 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
    10681 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
    10741 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
    10801 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
    10861 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
    10921 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10981 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    11041 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    11101 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    11161 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    11221 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    11281 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    11341 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    11401 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    11461 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    11521 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    11581 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    11641 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    11701 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    11761 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    11821 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    11881 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    11941 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    12001 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    12061 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    12121 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    12181 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    12241 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    12301 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    12361 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    12421 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    12481 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    12541 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    12601 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    12661 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    12721 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    12781 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    12841 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    12901 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12961 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    13021 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    13081 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    13141 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    13201 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    13261 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    13321 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    13381 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    13441 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    13501 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    13561 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    13621 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    13681 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    13741 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    13801 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    13861 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    13921 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13981 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    14041 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    14101 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    14161 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    14221 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    14281 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    14341 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    14401 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    14461 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    14521 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    14581 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    14641 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    14701 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    14761 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    14821 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    14881 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    14941 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    15001 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    15061 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    15121 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    15181 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    15241 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    15301 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    15361 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    15421 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    15481 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    15541 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    15601 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    15661 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    15721 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    15781 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    15841 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    15901 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15961 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    16021 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    16081 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    16141 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    16201 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    16261 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    16321 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    16381 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    16441 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    16501 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    16561 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    16621 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    16681 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    16741 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    16801 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    16861 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    16921 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16981 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    17041 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    17101 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    17161 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    17221 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    17281 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    17341 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    17401 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    17461 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    17521 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    17581 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    17641 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    17701 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    17761 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    17821 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    17881 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    17941 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    18001 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    18061 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    18121 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    18181 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    18241 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    18301 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    18361 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    18421 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    18481 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    18541 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    18601 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    18661 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    18721 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    18781 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    18841 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    18901 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18961 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    19021 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    19081 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    19141 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    19201 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    19261 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    19321 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    19381 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    19441 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    19501 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    19561 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    19621 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    19681 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    19741 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    19801 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    19861 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    19921 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19981 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    20041 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    20101 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    20161 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    20221 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    20281 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    20341 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    20401 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    20461 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    20521 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    20581 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    20641 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    20701 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    20761 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    20821 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    20881 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    20941 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    21001 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    21061 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    21121 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    21181 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    21241 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    21301 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    21361 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    21421 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    21481 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    21541 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    21601 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    21661 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    21721 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    21781 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    21841 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    21901 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21961 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    22021 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    22081 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    22141 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    22201 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    22261 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    22321 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    22381 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    22441 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    22501 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    22561 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    22621 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    22681 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    22741 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    22801 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    22861 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    22921 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22981 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    23041 attaaacact aaaactttcc gcattttttg ctcattaatt
//
LOCUS       DACDEA010000038        22697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDEA010000038 DACDEA010000000
VERSION     DACDEA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.038"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..542
                     /locus_tag="IV136_001598"
     CDS             <1..542
                     /locus_tag="IV136_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8465595.1"
                     /translation="QPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRMSDIIG
                     LKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRHIT
                     RDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRY
                     IGITEDEIGASLRGFKLGV"
     gene            complement(715..1188)
                     /locus_tag="IV136_001599"
     CDS             complement(715..1188)
                     /locus_tag="IV136_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8465596.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1200..1736
                     /locus_tag="IV136_001600"
     CDS             1200..1736
                     /locus_tag="IV136_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8465597.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1776..2147
                     /locus_tag="IV136_001601"
     CDS             1776..2147
                     /locus_tag="IV136_001601"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8465598.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2431..3702
                     /locus_tag="IV136_001602"
     CDS             2431..3702
                     /locus_tag="IV136_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8465599.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3947..4972
                     /locus_tag="IV136_001603"
     CDS             3947..4972
                     /locus_tag="IV136_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8465600.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5266..6375
                     /locus_tag="IV136_001604"
     CDS             5266..6375
                     /locus_tag="IV136_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8465601.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6458..7354
                     /locus_tag="IV136_001605"
                     /pseudo
     CDS             6458..7354
                     /locus_tag="IV136_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7370..8194
                     /locus_tag="IV136_001606"
     CDS             7370..8194
                     /locus_tag="IV136_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465602.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8226..9536
                     /locus_tag="IV136_001607"
     CDS             8226..9536
                     /locus_tag="IV136_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8465603.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9684..10943
                     /locus_tag="IV136_001608"
     CDS             9684..10943
                     /locus_tag="IV136_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8465604.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10953..11825
                     /locus_tag="IV136_001609"
     CDS             10953..11825
                     /locus_tag="IV136_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465605.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11837..12667
                     /locus_tag="IV136_001610"
     CDS             11837..12667
                     /locus_tag="IV136_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465606.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12707..14890
                     /locus_tag="IV136_001611"
     CDS             12707..14890
                     /locus_tag="IV136_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8465607.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14951..16171
                     /locus_tag="IV136_001612"
     CDS             14951..16171
                     /locus_tag="IV136_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8465608.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16168..17628
                     /gene="gtfA"
                     /locus_tag="IV136_001613"
     CDS             16168..17628
                     /gene="gtfA"
                     /locus_tag="IV136_001613"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8465609.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17705..18811
                     /locus_tag="IV136_001614"
                     /pseudo
     CDS             17705..18811
                     /locus_tag="IV136_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19133..19342
                     /locus_tag="IV136_001615"
     CDS             19133..19342
                     /locus_tag="IV136_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8465610.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19335..19484
                     /locus_tag="IV136_001616"
     CDS             19335..19484
                     /locus_tag="IV136_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8465611.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19519..20303
                     /locus_tag="IV136_001617"
                     /pseudo
     CDS             19519..20303
                     /locus_tag="IV136_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20587..21672)
                     /gene="xerS"
                     /locus_tag="IV136_001618"
     CDS             complement(20587..21672)
                     /gene="xerS"
                     /locus_tag="IV136_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8465612.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22006..22239
                     /locus_tag="IV136_001619"
     CDS             22006..22239
                     /locus_tag="IV136_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8465613.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22236..22643
                     /locus_tag="IV136_001620"
     CDS             22236..22643
                     /locus_tag="IV136_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8465614.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7741 a         3408 c         4364 g         7184 t
ORIGIN      
        1 tacaaccgtt aaggacaaat gaagagatac aagattttca gttttggcta agaagaacaa
       61 gtaatccgga aagagatagt tttttatttt tgtttggaat caataatggg ttacgtatgt
      121 cagatattat aggattaaaa gttggagata tacgaggcaa aagtaagcct attattgttg
      181 aacataaaac aggtaagcga aaaccgattt ttatagataa tttgagagaa gaaattttat
      241 tatatactga aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa caaggtcgac
      301 atataacaag ggatcgtgtc tatcagatat atgcagatat agcagaaaaa ttaggacgag
      361 atgatatagg aacacataca ttaagaaaaa cttttgggta tcactactac aagaaaacta
      421 gagacattgc taccttaatg tttattttta atcatagctc gcaagcaatt acaaaaaggt
      481 acattggtat aactgaagat gaaataggag catcacttcg tggatttaaa ctaggtgtat
      541 aatttaaaaa tttttataaa cagtaaagac aaagattaaa agctattttt atctagaacg
      601 attttatttc ataaagttcg cggaataaaa gctggggtta ctggtacttg gtataggttc
      661 gtgttatata aactaagtaa ttaaataaca tttttaggtt ggaaaaggat gaaattaaaa
      721 aaagagaatc aatacggtct caaaaacgca aacgctgcgg aataagaaaa atactgataa
      781 ctcaacgttt agcagactat ctaataggga tagtctgctt ttttcgtttt tgaccatact
      841 tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat ctgcaaaacg
      901 aattgccgtt tcctctgtct gctttttcgc gatatgggtg tatatatcat ccatagtggt
      961 tttaatatct gtatgtccaa gtcgaacata cacttctttg acagacgcac cagattcaaa
     1021 gagcagacta caatgggtat gacgaaaacc atgaagtgtg atatgtttta agttattctc
     1081 tttaatgatg attttcaaat tgtgatttac atagtctaaa taaaggggct tattttcttc
     1141 tgatgaaaac aaaagttgtt caccatcttt tataggtttg attctcattt ttagatatta
     1201 tgcccgcacg tattcatcac atttctattg tgaatcgctt tattcgaccg acatttgact
     1261 tttatcataa tatactcggt ttaaaattac taatgaaaac aattaatcaa gacgatcata
     1321 ccatgtatca cctgtttttt tcagataatc accaccgtgt gggaactgaa ctgacttttt
     1381 ttgaagtaca agaagggaac aaccagtttt ttggaaccaa tacgattgaa cgaacgatac
     1441 taaaagttcc ttccgaagca tcattgcatt tttgggaaat atattttgaa acacaaggcg
     1501 tttgccatta tggaatcgaa tcattttctg gacgacctat cttacgtttt gaagggccag
     1561 atgcaacacg attagcttta gttcccttac gcgaatttga agatattgat gattatttcc
     1621 cgtatgtcac agaccagatc cctacagagc atgccatttt aggaattgat gccattcaac
     1681 tacgcgtaca atattccgat gcgactgaac gagagttatt gtcggtaggc tggtagcaca
     1741 aagaatacgt cagtttcttt tcgggtcatc aacgagtgac aatcttagaa aatcaagaca
     1801 ctcagttcta tcaagaagtt cagattattc aagatcgtga gaatccagtt gccattgaag
     1861 ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat ttacaacgca
     1921 tcgacaaaca attacaagag cgcaatttta taaattcagg aataaaagat cgtgaatttt
     1981 ttgtatcact ttattatcgt gacccaaatc aattattgat tgaaattgct acaggggaag
     2041 gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa attcctttat
     2101 ttttaccgca gtacttaaat ttcattcgtg aacaagtgga gcaataatta gctaatcaaa
     2161 tccatgacga agagtaagag gttgactcat aagtcaaaaa ataaagaaaa acgcatcaaa
     2221 tccattctaa agaatgttga tttgatgcgg ttcctatttt tataaactat cattttcgac
     2281 taataaaact acttatgagt cagcctctct ttttttcttg atttttatac ctctttctag
     2341 gtaaactaaa catctgccaa aaataccgtg tctcattgaa acattctcaa ctatttcaaa
     2401 ataaagggga gtaccaaatg aacgagtata ttgaaattat ccacgcaaga gaaacaactt
     2461 aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta tttttaccag cgtatctggt
     2521 tcaggaaaat catcgattgt ttttgatact attacccaag aagctggtcg tcaactgaat
     2581 gacacctatg atagttttac ccgtttattt ttacctaaat acagtcgtcc agaggttgat
     2641 gagattgata atctttctac ggcgattgtg gtcaatcaaa agaagattgg tggcagtgcg
     2701 cgctcaacgt tagggacgat tactaacatc aatgcgttat ttcgtgtgtt gttttcatgt
     2761 tttgcaacac ctagtttagg atatgcaaat gcttattcat ttaatgatcc tagtggaatg
     2821 tgttctgtct gtcagggaat cggtaagacg attgcatttt taataggatt gctttatttt
     2881 tcggctattg ataatgctga ttttgtattt tctgatccat ttaccgcaga agttttgtct
     2941 ggaattgagg aacaggcaaa gaatcatggt tattttgtat tggtgcataa tgttaattca
     3001 gcagaagatg ttaaaactat tcagagaaat tggcgttttg aggggttcat tgttgttggt
     3061 gtacgtgtaa aagaattttt ggaattaaat aacgtttttg atggtcctgt tgtttatatt
     3121 gatacgcatc tttcaaaaga gacattaaga gaacttgaac aacaaatgaa tcgtttattt
     3181 gtaaatacaa atgattacca agctagtaca ttggcagtgg aacatttgat tcattatggt
     3241 catgagaaaa tagcgtttct ttcttttgac tacattgtag gtgaaccaag tgttattcaa
     3301 gaacgacatc aagcttatat cgatacatta aagaaatatc atttaacctt taaatcgcat
     3361 tggatatata acaactctga atttgagcga atttatcaag aattagctga atttactgca
     3421 attgttgtta ctggagatta tttagcggct aaattagtaa aatttttaaa aaataagaat
     3481 aaacgagtga tggaacaatt atcgattatt ggatttgatg atattacttt tgcagaatta
     3541 atggatcctg cgttgacaac aatacgacta aatccaggta acaagggggc gattgctttt
     3601 caccaactga ttggactggc caaacaagaa gaaataaaaa atcaagtact actattagag
     3661 ggagaattga tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc tgtgaaataa
     3721 gtatagttaa caataaaata tccgaacgat agagagaatg aactgtgatc tagcagttcc
     3781 tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa ttgttcggat attttttctt
     3841 gtttttaatt atgattacag tgcctatttt taacgaaatt tgcttatttt caaaatgtat
     3901 gattatatgt tagtaaacac tttcaaatat gaaaagagga gctattatgg taggaattag
     3961 ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc taaatgagga
     4021 tccgacactt tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg ttaattttta
     4081 tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat taataacgac
     4141 agtttctgaa gtagatgagt tggaagatcc ctattttcga acgattcgca gagggattca
     4201 agtcgaagca gaacaaagca aagtcaattt gaagaaaatt attcgtttgt cagaaacacc
     4261 gttggagcaa gacgatttgc taaaatgtca aggtgtgttg attattgggc aggtgctatc
     4321 ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg atgaccctag
     4381 tattgaagct gaagtagatg cagtttatac tgatttaaca aaagcaacga atcagcattt
     4441 agatcgattg tataaaaaag gacaccgaaa cattgcgttt atcggaggag gacgtgttcg
     4501 tttagatagc caaggaaata aacacgtgtc agatgatgat tatcgaaaaa tagcctatga
     4561 gacgtggatg aaacaaaaag gattagaaaa atatagtcaa atttatttag aaggctggac
     4621 cacacttgac ggaatgcagg cagcagattt acttttgaaa aaacatgcta aagataagtt
     4681 gccgactgct atcatggtag gaaatgatcc tattgctgtg ggggtttatc gttcacttca
     4741 aaaaaatggt ctacatattc cagaagatat ttcgattgtg agttttgata atattgaagt
     4801 tgctgaattt ttaacacctg cattaaatac ggtgaatgtg aatacagaag aaattggaaa
     4861 actagcagtt cgcatggtta atgaacgaat aaaagaagtt agaaatgttt cgatcaaagt
     4921 gattgtttct aataatatta ttgttagaga aagtgaaaaa aatatcgatt aaaaaagtaa
     4981 acatccaaaa aagggtgttt acttttttgt ttactaatat ttttagtaaa aaatctcgac
     5041 aaaacattaa gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt caaaacagga
     5101 ggaattaaga tgaaacattt caaaaaaata gctttaacag ggatagctgt tttaactggg
     5161 acattactcg cagcatgtgg tgggggagat agtaattcta aagacgggca agtaacttta
     5221 agctatgcga gttgagatac tgaccaagct ccaggactaa gaaaaatgtt agatgaattt
     5281 gaaaaacaaa atcctgatat caaagtagaa atggagacaa cgccttggga tcaatattgg
     5341 gttaaattag aagctgcaac aacgggaggt aatatgccag atgtagtaac catgcactct
     5401 agcgaatcct ataaatatat gtcacagaat gctttgatgg gacttgatga tgttatcaaa
     5461 gaaaatgata ttgacatggg aaactttact gaaggaattg ctgatttcta tacgcttgaa
     5521 gacgaactat acgcaatccc taaagatgct tctgtagtgg ggctatggta caacaaagaa
     5581 ttatttgata atgcaggtgt cgattatcca gatgaaacat ggacatggga aacactcaga
     5641 caagctgcaa ttgatttaac agatgaatca aaaggcatct atggttttgc atcacatatt
     5701 ggaacagaga ctggctactg gccatttatt tatcaaaatg ggggagaagt atttgccgat
     5761 gacaatacaa aatcaggttt gaatacccca gaagcacaag aagctttagc tttttatcga
     5821 gatattgcac ttgttgatca tgcttcacca acaattacag aattacaaga tcctgataag
     5881 ttaagtcgtt ttcaatcagg tagattagca atgatgattg aaggaaactg gtttgcaccg
     5941 ggatttttga aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt acctaaggga
     6001 aaagttaatg caacaattac aaatggactt ggttgggctg ccagtgcgag tacaaaatac
     6061 ccagaagaag tgaaaaaact aatggcattt ttagctagtg aggacgccaa taaaatccaa
     6121 gcggaaacgg gggcatcaat cccagcacta aaaggctttg aagacgagtg ggctgcttct
     6181 tcagatacaa ttgatttgag tgcttttgga aaaatgttgg agtatggagt ccctcgtcca
     6241 tacaataaag atggattacg agctgaacaa gcagaagttc aaattattaa ccgtattctg
     6301 tctggtgaag taactgttga agatggaacc gctcaaattg aaaaagaagt caatgcgata
     6361 ttgagcggaa attaaattag ttaattatac aatatacagg cgaaaggatt ctatttcgtc
     6421 tgtatattaa tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa tttttggaaa
     6481 atatcgtaaa gatcaagtga tatggggcta tatttttatt tttccaacgg cattaggatt
     6541 aattttatta aatatatggc cagcaattca aacagcaatt ttaagttttc aaaaaacggt
     6601 agggttcggt gatactgaat gggtcggatt aagaaattac caaaaaatgt ttgcagacgg
     6661 tgaagttttt cagtctttat taaacacatt aatttatgca gtggtttcga tacctagtat
     6721 tgttgtatta tccttgttgg tggcagtttt aatgaataga aaagttaaag gattgtcgat
     6781 ttatcggaca atttattttc taccagtcgt tgctgcacca tccgcagtcg caatgatttg
     6841 gcgctggatg ttcaataatg attatggact gattaataac atgttggcta aaataggtct
     6901 cgatggacca gcatggttaa cggatccatc gattgcgatt tattctatta tcattgtagg
     6961 agtttggagt gctattggtt ataacatggt gttattacta gccggcttat aagaaattcc
     7021 taaagattat tatgaagcag cggaaattga tggtgctagt ccaatccagc agtttttcaa
     7081 cattacgtta ccattagtta caccaacatt attttttgta gttgtaacaa cagttatcaa
     7141 tgctttccaa gtatttgatg ttatttttat gatgattact cctaatagta ccgccatgtt
     7201 taaatcacaa tcattagcct atttattcta taaacattca tttattttaa atgacaaggg
     7261 atatggttcg gcaatcgtta tgttcctttt agtgattata ttaattatta ctgcaattca
     7321 aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta tgaaaaataa
     7381 atttttttca aatgcgccaa tccatatcat gcttatttta ggtggtattg caatggcatt
     7441 accttttatt tggatgattt taacttcagg gaaaacgttg gttgaatcta cccaaattcc
     7501 acctacaatt ttccctgaga aatttcagct aagtaactat caacaagtac ttgatttatt
     7561 accattcatg gctttttata tcaatacatt tttaatggtt gttggacgag tgattggctc
     7621 ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc cagggaaaaa
     7681 tatccttttc agcttagtac ttattcaaat gatgattccc ggttccattt ttattactcc
     7741 acagtattta ttagcacaaa aattgggtat tttaaataca attccagcat taatattacc
     7801 aggggtagtt agtgcgtttg gtacgttttt attaagacaa tttttcttag gtttacctga
     7861 tgaattggag gaagcggcct atttagaagg tgccaatatt tggacgatct ttttcaaaat
     7921 tatgttgccg cttgctaaat caggattggt tgcacttgga atattcacag cgttgtttgc
     7981 atttaaagat ttaatgtggc cattgattgt caatacttca attgataaga tgcctttagc
     8041 tccgggatta gcaaacttac aaggacaata tgccacgaac ttcccacaat taatggcagg
     8101 ttcgttatta gcaatttggc caatagttgt gatattcatc gttttccaaa gaaaattcat
     8161 tgaaggaatt gctacaacag gtagtaaatt ataaaactat taattagtga ttggagagta
     8221 agtacatgac aaaaattaca tttattggtg caggaagtac tatttttgca aaaaacgtat
     8281 taggcgatgc gatgttaact ccaagtctac aagatgcaca tattgcattg tacgatattg
     8341 atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa aattcgaatg
     8401 aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga agcgttaaga gaggctgatt
     8461 ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc aacagtgatt gattttgata
     8521 ttcctaaaaa atatgggttg cgacaaacta ttggtgacac catggggatt ggtgggattt
     8581 ttagaaccct acggacacta ccagtaatgt tagactttgc taaagaaatg gaagaagttt
     8641 gtccaaatgc ttggttattg aactatacaa atccgatggc gatgttaacc atgggtgttt
     8701 taaaagcgac gagtattaag actgttggtt tatgtcatag cgttcaagta tgcgtaccag
     8761 aattgtttga gcatttagga atagccgatc aatatgactt aaatgacttt caatggaaaa
     8821 ttgcaggcat taatcatatg gcattcttgt tggaaattac aaaaaatggg gaagatttct
     8881 atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc acatccagat tctgtacgtt
     8941 ttgaattgtt gaaacgtttt ggttactata ttacagaatc aagtgaacac aatgcagaat
     9001 accatccata ctttattaaa tcaaaatacc cagaattgat tgacgaatta gggattccgt
     9061 tggatgaata cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc atgcgagagg
     9121 acattgtaaa taatggggag ttaacacata cgagaagccg tgaatatgct tcatatatca
     9181 tggattctat tacgacagga actccgtcag tgattgcagg aaatgtctta aataaaggct
     9241 taatcacaaa cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc gataaaaatg
     9301 gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt agctgcttta aatcggacga
     9361 atatcaatgt tcaagaactg acagtagaag ccgctttaac actagataaa tcaaaaattt
     9421 atcaagcagc gtatttagat ccacatctat catctgaatt gtctttaaca gatatcaaaa
     9481 atttagtgga tgatttaatc gaagcacatg gggactattt acctgaatat aattaaaaag
     9541 aagctgaaag agtaaggaat tttcaattcc ttactctttt cttagttaaa tttagtaaaa
     9601 atattgacaa aacacttagt aaacagttac attagtgtta acaaaaagaa aacgcattca
     9661 aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa aattatttta gcttgtatag
     9721 cactaggagc aacgatctct ttgactgctt gtggaaaaaa tcaggaagat gcaagcggta
     9781 aggtagaaat agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt caggagattg
     9841 ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat gtaccgaatg
     9901 ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt accagatgtc attaatcttt
     9961 atcctcaaag tatagaattg aaagaatggg cgaaagctgg ttatttagag gacttaaccg
    10021 aagaaccgta tttagagaat atcaaaaatg gatatgcaca acgttttgct attgaggatc
    10081 gtgtatatag cataccatta accgctaatg tctatggttt ttactataac aaaactgctt
    10141 ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa atagtagctg
    10201 agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc tggactttaa
    10261 atggttatca ccaactagct atagcgactg ctgctggtgg tgaagaggaa gcgaacaatg
    10321 tttggcgttt ctcagaagtt aatggaatta atgctgaaag caaagaaatg caaaaagatt
    10381 ttaatcgatt agatttgtta agagaaccaa aagctttgca aaacaactgg caaggtgccg
    10441 gttataacga tacagtagtt acttttacaa aaggtgatgc gttgattatg ccgaatggtt
    10501 catgggcaat gccgatgatt cattcacaaa atccagattt tgaagtagga acttttcctt
    10561 ttccagcaga tgaagcaggt caaagtctaa cgattggcgc aggagaccta gccttatcag
    10621 tttctgcaac ttctcaacat aaagaagaag ccaaacgttt tgtagaatat atgacaacac
    10681 ctgaagcgat gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt gagggcgtta
    10741 tcgagaatac tcaggattca ccattaagtg gtttaactga acttgctttt actgatcgtc
    10801 atttagtttg gttagccaaa gattggaata gtgaaaatga tttttatacc ttaacaacaa
    10861 attatttaca caatggcaat caaaaagcca tgattaacgc attaaatgct ttctttaatc
    10921 caatgaaagc tgacggaagg taggaaaata aaatgaaaat aaaagaaatt ttaaatcgtt
    10981 actgggcaat catttttgta gcaattccaa ttattttgca gatgattttt ttctactttc
    11041 caatggttca aggtgctttt tacagtttaa ctaattggac tggtctgact tataattttg
    11101 attttgtggg tttgaacaat tataagattc ttttttctga tccgaagttt atcaaatcga
    11161 ttggcttcac tttaattgtg acgctttgca tgattgctgg acaaattggt tttggtattt
    11221 tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac attctttaga gcatggttct
    11281 tttttccagc ggtcttatcg ggcgtggttg tttctttagt atttaaacaa atttttaact
    11341 atggtttacc aaaaattggt agtatgttag gcattgaatt tttacaaacc agtcttttag
    11401 gaacaacatg gggagcgatt tttgcagtaa tttttgtttt actttggcaa ggaattgcca
    11461 tgccagtcat cattttctta gcgggttttc aaagtattcc acaagaaatt attgaagctg
    11521 ctagaatgga tggggcgaat gataagcaaa ttttttggaa tattgaaatc acctacttat
    11581 taccaagtat ttcaatggta tttattatgg cattaaaagg tggcttaaca gcatttgatc
    11641 aaatttttgc tttaactggt ggtggaccaa gcgatgccac aacatctttg gggttacttg
    11701 tttataatta tgcttttaaa aacaatcagt ttggttatgc gaatgctatt gctttagtct
    11761 tatttatctt aattgggatt gtttcactgg cacaattaaa attatcgaaa aaatttgaaa
    11821 tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat atttctaaat acttgctttt
    11881 attattaggg ggcttcatga ttttagttcc cttagtttca acgctattcg gttcatttaa
    11941 aacaacaccc gatattatga ataatttctt tcgttttcct aatccagcga ctttggaaaa
    12001 tttccgaaga ttgttagcgg atggaattgg tcattacttt tggaattcgg caatcatcac
    12061 aatcattgca gtagttgctg tgacagcaat tattcctatg gctgcttatt cactagcaag
    12121 aaaaatgact cgtgggaaag catacggatt gatgtattta tttttaattt taggaatttt
    12181 tgttcctttc caagtaatta tgattccgat taccgttatg atgagtaaaa ttggtttagc
    12241 aaatatgtgg ggattaatct tactctatct cacttatgcc gtaccacaga cactattttt
    12301 atatgttggt tttattaaac taaatgtgcc tatcagtctg gatgaggctg ctgaaattga
    12361 tggggctagt cactttacaa cttatttcaa aattgttttc ccaatgttaa aaccaatgca
    12421 tgcgacgact attattctta atgctctatg gttttggaat gatttcatgc taccattatt
    12481 aattttaaat cgtgattcta atatgtggac actaccattg tttcaatata attatacggg
    12541 acaatatttt aacgactatg gtccaagttt tgcttcctat acagtaggaa ttgtcacgat
    12601 tacactagtt tatcttgctt tccaaagaag tattatagct ggaatgagca atggagcagt
    12661 gaaataacca ataaattatt ctcattataa aggagttcat aaacttatga ttatatttga
    12721 caatcacaca caaacatttc atttacaaaa taaaaaaatt agctatctat taggaatcga
    12781 agaaaataat tatttaacgc atctttattt tggaaaaaag attacgcact attctggtgg
    12841 gtatcgctac ccccgaacag atcgttcctt ctcacccaac aatgcaaatg cgcaagatcg
    12901 tttattatct ttagacacat taccattaga atttccagga tatggacatg gtgattttag
    12961 agaaccggcg cataatatta aattaggaaa cggtagtcgt gtaaatgatt ttcgttatga
    13021 tagctacgaa attcaaaaag gaaagcccag cttagcggga ttgccagcct tatatacgga
    13081 agatgatgat gaagctgaaa cgctgattat tcaattgaaa gacgcgatta gtggattgac
    13141 tttacagttg ttctacacaa tttatcaaaa tcacagtgta attattcgtt ctacgcaatt
    13201 agtaaataat agtgatgaaa cagtggagat taaccgctta gctagtcaat caattgattt
    13261 tccaaatcgt ccactcgagt taattcattt aaatggtacc tgggcaagag aacgtcaaat
    13321 gactagagaa aaaattcata cagggataaa agtattagat agtaaacgcg gatcaagcag
    13381 ccatcaacaa aatccgtttg ttgctttagt agatgccaaa acagatgaat ttcaaggaga
    13441 ggcatatgga tttaatttag tttatagcgg taatcacgaa acggtgattc aaaaagatcc
    13501 atttgaccaa acacgggtca ttatgggaat taattcattt aattttggtt ggcaactaaa
    13561 aactggcgac tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg aaggtctgaa
    13621 tacgttatca cagacgtatc atgaattgta taatcaccgt ttaattcgtg gaaactatcg
    13681 tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca acgtattttg attttaacga
    13741 agaaaaaatc atgaatattg tcgatgatgc gaagaacttg ggcattgaaa tgtttgtttt
    13801 agatgatggt tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg attgggttga
    13861 aacagaaggc aaacttgctt gtggtttgga aaatttagcc aagcaagtgc atgataacgg
    13921 gatgaaattt gggatttggt ttgaaccgga aatgatttct gaagatagtg aactatttcg
    13981 agcacatcca gattgggcat tgggtattcc aggtcgtgac cgctcaatta gtcgaaatca
    14041 atatgttctt gatttttcaa gacaagatgt tcgtgataat atctatcaac aaatgacggc
    14101 gatttttgat cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa atatgacaga
    14161 agtctattct gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc gttatatgtt
    14221 agggttatat gaatttttag aaaagttaac aacgaattat ccaactattt tatttgaaag
    14281 ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg tattacatgc cacaaacttg
    14341 gacgagcgac aataccgatg ccgtggctcg tctgaaaatt caatatggaa ccagcttagt
    14401 gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc aaacaggaag
    14461 agaaacgagt ttaactattc gtggcaatgc tgcgatggct ggtgtttttg gttatgagct
    14521 agatctaacg caattaactt ctgaagaaaa agcagaaatt aaagaacaag ttgcctttta
    14581 taaaaagcat cgccaacttt tgcaatatgg aactttttat cgcttggaaa gtccgtttga
    14641 aggcaatgat actgcatgga tgtttgtctc aaaagaccaa acagaagcca tggtctttta
    14701 tttccgagta ttagcagaag ctgcgcatcc attagtcaca ttgaaattag caggattagc
    14761 agaagaagca acttatcaaa tggaagatgt aacatttgct ggcgatgaat tgatgaatct
    14821 cggtttttat attgactcag aactacatgg tgattatgcg acacaacgct tttatttgaa
    14881 acagatatag tagcttgaat ttacaatata agtgcaacac caaaagagac tcattccaaa
    14941 cgaagaaaat atgttttatt tacacgcatt tactaaaaag cagcctgatt tgaattggga
    15001 aaatgaagaa gtacgtgaag aaatctatgc aatgattaac tattggttag ataaaggact
    15061 aggcggattt agaatcgatg cgatattaaa catcaaaaaa aaactagagt atggcatctt
    15121 tgaatctgat ggagaagatg ggttagcctt cattgggccc tggattctta atcagtcggg
    15181 cattgaagtg tggttaaaag aaatgagaga gcggacattt aaaccacata atagtatgac
    15241 ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa tatattggtg aagatggcta
    15301 ttattcgatg gtttttgatt ttagttatac ggatattgat gtacctgaaa ctggtgaatg
    15361 gtttaaagat agccaatgga catggacgga tatgcgtacc aatattttta cgaatcaatt
    15421 agttacgcaa gataaaggtt ggggcgcctt atatttagaa aaccacgatc aaccacgttc
    15481 aattaataaa tatattccag aagaatggat caatgattat agtaaaaaaa tgttggcgac
    15541 gctttttatg ttactaagag gtaccccttt tatttatcaa ggacaagaac taggaatgac
    15601 caacatagaa atggcatcat tagaagattt tgatgatgta gctacgcatt ctcagtataa
    15661 acgggcatta gaatatggat tggcaccaga gcaagcattg aaagctgtca ataaacgaag
    15721 tcgggataat tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca gattctcaac
    15781 ttctgataat gtatggttaa aagtaaatcc taattatcca gaactcaatg cagaagctca
    15841 gttaaccaat caacagtcgg tattcaatta ttatcatcaa ttaattaaat taagaaaaaa
    15901 tagtgtatac aaagacgtgc taatctacgg acaatttcta ccagtgaaag ataaaaatga
    15961 acatattttg ctttatgaac gccaattaga agataaaatc gtattagtta tcttaaacgt
    16021 tacagcaaca gaacaagaat attatgtaga tgaacagtac caacaagtat taatcaataa
    16081 ttatgaagat gttttattag atcaaagtcg aaaattgcgt ttacgaccgt ttgaaagtat
    16141 tgtattagct aattatggag gacaattatg aaaattaaaa acgaagcaat gctcatcacc
    16201 tatgcagata gtttaggaaa aaacatgggc gaattaaaac aagtcttaga caaacattta
    16261 caaggtgtta ttggtggtgt acatctgttg cctttttttc catcgacagg tgatcgaggc
    16321 tttgcgccga gtgattatac gacagttgat ccagctcttg gaacatggga agatgtggaa
    16381 gcattaggtg aagattatta tttgatgttt gattttatga ttaatcacat ttctcgtgaa
    16441 tcaaaatttt tccaagattt caaaaaaaac catgagcagt caccatacaa agaaatgttt
    16501 atccgtattc atgaattttt cccagaaaat cgaccaacac aagaagatat tgatttaatc
    16561 tacaagcgta aagacaaagc gccgtttcaa acggtagaat ttgcggatgg tacgacagaa
    16621 gaggtttgga atacctttgg ggaagaacaa attgatttag atgtgaccaa agaagttgtg
    16681 aaagaattta ttcgagaaac catcaaagat atggcgaacc atggctgttc tttaattcga
    16741 ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca atgatttctt cgtggaacca
    16801 gatatttggg aacttttaga cgaagtgcgt caagaagcag cgaaatatga cgtcgaatta
    16861 ttaccagaaa tccatgagca ttattccatt caaatgaaaa ttgccaatca tgattattat
    16921 atctatgact tcgccttgcc aatggtcact ctgtattcac tttatagtgg aaaatcagaa
    16981 cgtttagcca attggttgaa aatgagtccg atgaagcagt ttacaacatt ggatacgcat
    17041 gatggaattg gtgttgtgga cgctcgtgat ttattaacag atgaagaatt ggattacacc
    17101 tctgaggaat tgtataaagt cggggcgaat gtgaagaagg tctattcaag tgccaattac
    17161 aataatttag atatttatca aattaacagc acctattata gtgcattagg cgataatgac
    17221 cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc cagggattcc acaaatttat
    17281 tacgttgggt tattagcagg aaaaaatgat attgaactgt tggaagcaac gaaagaaggg
    17341 cgtaatatta atcgtcatta ctatgattta gccgaaattg aagaagaagt gcaacggcca
    17401 gtgattcaac aattatttgc acttttaaaa tttagaaatg aatctgcggc gtttgatttg
    17461 gatggaacga ttgaagtagc aacgccatca gaatcagaaa tagtgattac acgtaaaaat
    17521 aaaaacggta gtgatagtgc aacattaacg gctaacttgg atacgaaaga atttatcatt
    17581 gagtctaaag atataaaaat tttcactagt gttgcattaa aattttaacc tattccatag
    17641 aacaaaaaat cctttatact agcacttgga tgacttatcc aagaccaata aaaaggactc
    17701 atacatggat aagtataaaa caaatacagc atttaataaa tggttttctg ccataaaatt
    17761 aggaaaactt tctgtcaact cccaaaaatg tatttttgat tacaattcgt atcgtaaaaa
    17821 actgtctttt gaagctgtgc tcaagctctt tctctacgca gttaatgaag aaaaagaaag
    17881 tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc caactagaaa ccgatgtgtc
    17941 atcgattagt catacccaat tgtctcgagc atttaatgca cttgaaccta aagtgttaga
    18001 agaaattttt caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa ccaagcgaaa
    18061 tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc ataagtgggc
    18121 gactttccgt caaacaaaat caggtgtcaa gttacactta aactattgtt atatggacga
    18181 tactacgatg tatccaactg attttacctt aacaaatgcc catgaacatg atctcaatca
    18241 actaccagta ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg ggtattagga
    18301 ctttgaaaag atggatcaga tgcattggga tggctatttc ttcgtcactc ggattaaaaa
    18361 aaacaccaaa gttcacgtga tagatactct ggaaacatcg ccagaaacag aaattctacg
    18421 agacgagctg gttcgtttag gatctaaaac ctatctaacc gccaatttcc gactagtcac
    18481 tgttcaagat aagaacggac gtgtgtttca atttatcacc aatcgaatgg acgtctcttc
    18541 aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc gaattgtttt tcaagcacat
    18601 caaacaacac atgaccatta aaaccttctt ttctcaaagt gaaaaaggcg tgcagaatca
    18661 attgatttta acgatgattt ccgctctcct cacgtttctc atcaaattgg agacgaaaac
    18721 ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat ttactgtttc agccctttga
    18781 atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga aatttggctc gttacttttg
    18841 gttttcagtt gaataacaga taaaaaattt tctggaaaaa gtcatcctct gcgtcagcac
    18901 agtttttttt gacttatttt cagaggaaat agttttcaga atattctaaa atgttttatg
    18961 caacactagt gatatttttt agaattagat ttctaatgtg cattttttga gtattaagga
    19021 gcaagtatac aaagatttat aaagctgatt aaatttgaat aaaaatatgc aggaacgcta
    19081 ctatttttag tagtgttctt ttttatattt gaataaaaaa tgtggtggtt aaatggtggt
    19141 tataacaaaa aatgaattaa tgaaattagg ttatgggaag tatcagtctg agagaatcat
    19201 acgacaggca aaagcaatta tggtttcaaa aagctatacc tattacgaaa atagaagatt
    19261 aggactagta cctgttgaat ctgtagaaga agttttaggt atgaagatag aggtagagga
    19321 ggattttcag aatcatggtt aattttaaaa aagataaaaa tgggaaatat cattttactg
    19381 ctagtttggg attcgacaaa cgaacaggga aaagagtaca aaaaaacgta gaggggttaa
    19441 aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt ctaattatgg aaaagaagca
    19501 gaaacttctc aaagtactat gttgtttgag gagttttttt ataaattatt cctaccttat
    19561 tatcaaggga aagtagaaga aagaacatat aaaaatcggt tatctacaat caaaaaaact
    19621 tttcttattt ctttaaaatg gagttaagag aaatacgtcc aattgacatt caaaagtggc
    19681 aaaataattt attgagcaaa tataaaaatt cttatgtaag aaacatgtat gggatttttc
    19741 agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct gcaaaagtta
    19801 ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa gaatttacaa
    19861 aagttatttc aactttttat attgatgatt tgtaccaaca ttttggtttt atttgtatgt
    19921 ggttactttt tatgaccgtt atgcgtttgg gagaagccac tgcattaact tggaaagata
    19981 ttgattttga aaataaaaac ttgcgtgtga ataaaacact ctattataag agagccaaaa
    20041 cctatgagtt tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa ctagacgaca
    20101 atacaattac ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga ggcatgtcgt
    20161 ttgtactttc ttataatgga acacctactc agaaatatac aatccgtcat attatgagaa
    20221 gacatgcaac gatcgttggt gttcatataa tccgcataca cggattaagg catagtcatg
    20281 cttctttgct tatatcacaa taaaaagatg cacttcgaat taaagaaagg ttaaaacatg
    20341 ctgatgtaca aacgacttta ggaacttatg gtcatttgta tctaaattca ttgagagaga
    20401 taacaaatga tcccacagat tatatccaaa ctaatttttc taaaatcaaa ccgacaccta
    20461 tttacgataa taaaaaataa atgataaaca aaagtaggat cacaccaaaa gaaacacact
    20521 agaaaaacta taatacaggc ttttctagcg tgttgtttct tatttccgcc tatttataca
    20581 ttatactcat aaagcattta atgcatcgat cgtagccgca ttgtctatat gggtatataa
    20641 agttgttgta ttggtacttg aatgtcccaa ttgttgggca gttaatacca atgaatttgt
    20701 ctgttggtag agtctagtag ctaatgtatg acgcagtttg tgagggctga cttgaattct
    20761 aaagcctttt gaatattttg ctaccatccg ttcgattgca ataccggata tacgcttggc
    20821 ttctccttgg taaattgcta gaaataaagc tttttcgttt tctgttactt tatatcgttc
    20881 tttccgaata gctagataat tagctaaata ttccattgcg aatgctgcta tattgacagc
    20941 atctctttta tttccttttc tagttactac cacattattc ttatctaaag ataggtcatc
    21001 taaattaata tttataacct cggagagtct caatccagat cctaaaatca gtgcattgat
    21061 cgccaggtct ctttctacat ctcgttgata atagatcaaa gctcgtttac tcaaagtatt
    21121 tttatattca ttggcaatat aatctagata attttgtgta tccttatcta gaaataattt
    21181 tttctcaatg gcattggcac gtgcagctaa cgtagtttta tctgtgatgg ttgctacttg
    21241 atccatagga ttattgtcta agtatggttt tcctagagga ttattggaac ttttcgaaag
    21301 atatttaaat agaaccttta atgccgtgat atttctattg atagtagaag tagatagacc
    21361 taagttatta ttatctttga gcttagtttt tgttgtctct ttttttacat tttcttttgt
    21421 gcgacgtttc aaatgagatt tgtacaagtt taggtcatag gcagttaact gtcttatcga
    21481 ggtgatagaa gtatcaggaa cgatagactc actcagaagc cattcataaa agagcttata
    21541 attttttaga tattctaaag tggttcgagg tgattgatct ttatcctcac gatcttgaac
    21601 aaaatctttt atataatcgg gcatatgaag tatgattgta tctatttttt gatatatttc
    21661 tgtatttttc atgtcattca tcctttcaat aagtagggga gaagagtatc tctataattg
    21721 gactatacca attatagaat gttttgtcaa ttggtatgct ctaaaattta ttaatttgtt
    21781 tcaaaaaaag catataaaca ggtatacgtt taataattta aaataaatgg tatacataaa
    21841 tagggaagga aatagaaggg agcaatctaa attttttgaa ttttcttaat taattatata
    21901 gaaagaacaa tctttctata gagttagaca tgagagggac tatgcttgat ttaaaatcaa
    21961 caagcgtata tactgtatat acaaagaaaa aggagggaga agactatggc aattaaagaa
    22021 agaaaattta gggaagtggg gaactcagta gtattaacta tttcaaagga gtttcttaaa
    22081 gaaaatgggt taaatcccgg agatacggtt tttttagatg aagaaaaact tcgagcggca
    22141 attacaaaaa aacaagcctt atctgatgaa attgatttag caatatctca agcattaact
    22201 gaatatgata ctacttttag agagcttgtc gataaatgag tattcattac ctagaagcca
    22261 aagatattat aaaaatgaat gtaatccaaa taaaaaaata ctctcctaat gaaccccttg
    22321 gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca acttcgagcc tcagcttttg
    22381 gtgaagaggt ttatccttcg atttatgaaa aagcagctat cctacttata caattgataa
    22441 agaaacatcc ttttcacaat gcaaataaac gtactgcttt cttagctact tttgtctttt
    22501 tgaaaatcaa tggacagctt ttagctattg atcaaaaaga tgctgtaaat ttagcggtat
    22561 atattgctac gtatgataag gatttcgatc aactaaaata tgaagtgatc aacactataa
    22621 agacacatac gcagcccatt taaggagtta ctgtatgtgt ctttataggg ttattagctg
    22681 ctatagcgaa ataattg
//
LOCUS       DACDEA010000039        22617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDEA010000039 DACDEA010000000
VERSION     DACDEA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.039"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IV136_001621"
     CDS             4..816
                     /locus_tag="IV136_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8465615.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IV136_001622"
     CDS             813..1613
                     /locus_tag="IV136_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8465616.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IV136_001623"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IV136_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8465617.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IV136_001624"
     CDS             3110..4819
                     /locus_tag="IV136_001624"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8465618.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IV136_001625"
     CDS             5031..9383
                     /locus_tag="IV136_001625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8465619.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IV136_001626"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IV136_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8465620.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IV136_001627"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IV136_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8465621.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IV136_001628"
     CDS             11221..11514
                     /locus_tag="IV136_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8465622.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IV136_001629"
     CDS             11511..11822
                     /locus_tag="IV136_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8465623.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IV136_001630"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IV136_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8465624.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IV136_001631"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IV136_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8465625.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IV136_001632"
     CDS             complement(14623..14844)
                     /locus_tag="IV136_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465626.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IV136_001633"
     CDS             15292..17655
                     /locus_tag="IV136_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8465627.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IV136_001634"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IV136_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8465628.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IV136_001635"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IV136_001635"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8465629.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="IV136_001636"
     CDS             20243..21028
                     /locus_tag="IV136_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8465630.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="IV136_001637"
     CDS             21041..21892
                     /locus_tag="IV136_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8465631.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="IV136_001638"
     CDS             complement(22016..22468)
                     /locus_tag="IV136_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8465632.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7674 a         3948 c         4950 g         6045 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcc
//
LOCUS       DACDEA010000040        22160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDEA010000040 DACDEA010000000
VERSION     DACDEA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.040"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="IV136_001639"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="IV136_001639"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8465633.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="IV136_001640"
     CDS             2370..3848
                     /locus_tag="IV136_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8465634.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="IV136_001641"
     CDS             3814..5145
                     /locus_tag="IV136_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8465635.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="IV136_001642"
     CDS             5259..7811
                     /locus_tag="IV136_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8465636.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="IV136_001643"
     CDS             complement(7818..9257)
                     /locus_tag="IV136_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8465637.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9603..9965
                     /locus_tag="IV136_001644"
     CDS             9603..9965
                     /locus_tag="IV136_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465638.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="IV136_001645"
     CDS             complement(10152..11009)
                     /locus_tag="IV136_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8465639.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="IV136_001646"
     CDS             11129..12040
                     /locus_tag="IV136_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8465640.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="IV136_001647"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="IV136_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8465641.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="IV136_001648"
     CDS             13795..14094
                     /locus_tag="IV136_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8465642.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="IV136_001649"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="IV136_001649"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8465643.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="IV136_001650"
     CDS             15764..16357
                     /locus_tag="IV136_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8465644.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="IV136_001651"
     CDS             16381..17457
                     /locus_tag="IV136_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8465645.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="IV136_001652"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="IV136_001652"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8465646.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="IV136_001653"
     CDS             18462..19226
                     /locus_tag="IV136_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8465647.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="IV136_001654"
     CDS             19715..21808
                     /locus_tag="IV136_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8465648.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="IV136_001655"
     CDS             21833..22135
                     /locus_tag="IV136_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8465649.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4721 g         6476 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa
//
LOCUS       DACDEA010000041        21688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDEA010000041 DACDEA010000000
VERSION     DACDEA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.041"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1010..1555
                     /locus_tag="IV136_001656"
     CDS             1010..1555
                     /locus_tag="IV136_001656"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465650.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1556..1873
                     /locus_tag="IV136_001657"
     CDS             1556..1873
                     /locus_tag="IV136_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8465651.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2030..2194)
                     /locus_tag="IV136_001658"
     CDS             complement(2030..2194)
                     /locus_tag="IV136_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAP8465652.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2296..2817
                     /locus_tag="IV136_001659"
     CDS             2296..2817
                     /locus_tag="IV136_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8465653.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2789..3244
                     /locus_tag="IV136_001660"
                     /pseudo
     CDS             2789..3244
                     /locus_tag="IV136_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3284..3442)
                     /locus_tag="IV136_001661"
     CDS             complement(3284..3442)
                     /locus_tag="IV136_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAP8465654.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3535..4296
                     /locus_tag="IV136_001662"
     CDS             3535..4296
                     /locus_tag="IV136_001662"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8465655.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4483..5835
                     /locus_tag="IV136_001663"
                     /pseudo
     CDS             4483..5835
                     /locus_tag="IV136_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5851..7071
                     /locus_tag="IV136_001664"
     CDS             5851..7071
                     /locus_tag="IV136_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8465656.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7106..7783
                     /locus_tag="IV136_001665"
     CDS             7106..7783
                     /locus_tag="IV136_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8465657.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7780..8973
                     /locus_tag="IV136_001666"
     CDS             7780..8973
                     /locus_tag="IV136_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8465658.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8988..9314
                     /locus_tag="IV136_001667"
     CDS             8988..9314
                     /locus_tag="IV136_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8465659.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9318..9653
                     /locus_tag="IV136_001668"
     CDS             9318..9653
                     /locus_tag="IV136_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8465660.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9650..10045
                     /locus_tag="IV136_001669"
     CDS             9650..10045
                     /locus_tag="IV136_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465661.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10042..10413
                     /locus_tag="IV136_001670"
     CDS             10042..10413
                     /locus_tag="IV136_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465662.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10429..10989
                     /locus_tag="IV136_001671"
     CDS             10429..10989
                     /locus_tag="IV136_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8465663.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11114..11425
                     /locus_tag="IV136_001672"
     CDS             11114..11425
                     /locus_tag="IV136_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465664.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11449..11640
                     /locus_tag="IV136_001673"
     CDS             11449..11640
                     /locus_tag="IV136_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465665.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11640..15496
                     /locus_tag="IV136_001674"
                     /pseudo
     CDS             11640..15496
                     /locus_tag="IV136_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            15497..16369
                     /locus_tag="IV136_001675"
     CDS             15497..16369
                     /locus_tag="IV136_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465666.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16366..17415
                     /locus_tag="IV136_001676"
     CDS             16366..17415
                     /locus_tag="IV136_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465667.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17419..18243
                     /locus_tag="IV136_001677"
     CDS             17419..18243
                     /locus_tag="IV136_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465668.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18261..20321
                     /locus_tag="IV136_001678"
     CDS             18261..20321
                     /locus_tag="IV136_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8465669.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20484..20930
                     /locus_tag="IV136_001679"
     CDS             20484..20930
                     /locus_tag="IV136_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465670.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20932..21069
                     /locus_tag="IV136_001680"
     CDS             20932..21069
                     /locus_tag="IV136_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8465671.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21104..21346
                     /locus_tag="IV136_001681"
     CDS             21104..21346
                     /locus_tag="IV136_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8465672.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21362..21559
                     /locus_tag="IV136_001682"
     CDS             21362..21559
                     /locus_tag="IV136_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8465673.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21570..>21688
                     /locus_tag="IV136_001683"
     CDS             21570..>21688
                     /locus_tag="IV136_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8465674.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT         7509 a         3690 c         4833 g         5656 t
ORIGIN      
        1 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
       61 attcacacga taactaaagg tgagcgaaga gaaatgattc cattaataat ttcaattttt
      121 gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa aagatagtag
      181 taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg ctaagcatgt
      241 gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt gactttggca
      301 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
      361 cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat tttacataaa
      421 ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag taatcactgt
      481 gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata
      541 taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag ccaaatgaga
      601 aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg
      661 cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat gctaagagaa
      721 gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg ctattggaaa
      781 gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac ctcgaacatt
      841 tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact gtagatgatg
      901 ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc agtaaggagc
      961 agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa tgcacatgca
     1021 cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga acgacttagt
     1081 taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa aaaagcaacg
     1141 cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct tagaatactt
     1201 tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga caggcgaaac
     1261 aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata acaaacaaga
     1321 acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg tttggtacaa
     1381 gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc attggaagac
     1441 tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat acttttctag
     1501 ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata tgtaaatgaa
     1561 agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat ggcgagacct
     1621 aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc gtcgagggcg
     1681 gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc tgtcactgtt
     1741 ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag agcatccaga
     1801 gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa tgtattcaac
     1861 tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa agattaaaga
     1921 gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt ttcaagtttt
     1981 ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc tatttcttag
     2041 tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca atttttactt
     2101 cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt ccttttggtt
     2161 tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa caaatttcgg
     2221 cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat ctatcttttg
     2281 aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt attaaacgga
     2341 aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga agacaaatta
     2401 aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg aaaggatacc
     2461 tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc ggacgtccat
     2521 acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa acagcttcga
     2581 aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc agaggcgaga
     2641 ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg gcagatggat
     2701 acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc agcagccaga
     2761 gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga cttctaatat
     2821 tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg aggagcaagc
     2881 ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt ggtatgcgga
     2941 acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg ctaaacggca
     3001 tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg acgaagaaaa
     3061 aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag gcgactttgg
     3121 ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg gatgggtaca
     3181 tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac gtaaaaacgg
     3241 taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt tggctttctt
     3301 ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata gtttcttccg
     3361 acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt ctaattctgt
     3421 tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata acatccgatt
     3481 ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag tagattgaaa
     3541 aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat cggtgtcaca
     3601 aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc aaagaacgaa
     3661 acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga aaattttgat
     3721 catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga aaagttttat
     3781 attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa aggcggaaat
     3841 ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca atctaaagaa
     3901 ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat gacaaacggt
     3961 gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa gcaccacagc
     4021 aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac ctcaaaacca
     4081 attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa gctttgcatg
     4141 gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg cgccccttat
     4201 gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat cgaaggaatt
     4261 atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg ttactaacta
     4321 ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac agcagataat
     4381 ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa tgagggtgac
     4441 ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc actaatttca
     4501 ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt ttacgtattg
     4561 gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat ggtcaaacaa
     4621 tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa ttatgctccc
     4681 gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg gctaaacact
     4741 cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat caacgttatc
     4801 aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac tgctggatat
     4861 gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt ggaacatttg
     4921 gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag tgcggaagag
     4981 gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat gaattttgtt
     5041 ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc tgactggatt
     5101 accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga tatgcctacc
     5161 attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa gtgtgtcggt
     5221 ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt tccgcttgaa
     5281 acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt tgatcgagat
     5341 aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat tattcctggt
     5401 gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa aatctatgac
     5461 attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga actagaaaat
     5521 gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg gccaatgcaa
     5581 aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa gctttaccga
     5641 tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat gccgtctaag
     5701 cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca cgccgaagtg
     5761 ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc gatttccgat
     5821 ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt gcgttctttt
     5881 ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc gggattgata
     5941 ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat tagccgtgta
     6001 tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg ccaaccggac
     6061 gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa gtttgatttt
     6121 ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca gattttccga
     6181 aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg ccatccagtg
     6241 attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa aggcagttac
     6301 aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc gaggtttggc
     6361 ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata tgatcgagaa
     6421 gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa agttaagttt
     6481 gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc tgatttttat
     6541 cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg tttacaacga
     6601 gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag aatcgctatg
     6661 gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc ctctcaggaa
     6721 cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca atatgaagcg
     6781 gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg atataagttt
     6841 aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta tcaaatcatg
     6901 cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt gcaaccgata
     6961 aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat cgatatggat
     7021 ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta ggttttttta
     7081 gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg atgtcgtgga
     7141 taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac cagggaaaat
     7201 ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct catcgaacgg
     7261 tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc cgggaaaggt
     7321 atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt gcgcttgcga
     7381 accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg tgaccactag
     7441 tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg atgagtctat
     7501 tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg atttaatggc
     7561 gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag atgaaattat
     7621 gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc caaaaaacgc
     7681 agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag aaacactctt
     7741 acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga acaattaaaa
     7801 aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact caatgcagca
     7861 ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca agtaaaagca
     7921 gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc cgaacaagtc
     7981 ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa caagtttatc
     8041 aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt gacatctaat
     8101 acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca aacgactatt
     8161 catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga aaaagtgacc
     8221 actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt gactgctttg
     8281 gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt gatcaaatac
     8341 ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa agatactgcc
     8401 gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt tactcgcaat
     8461 acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga tgttacagat
     8521 gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga agctacatct
     8581 atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg tgctgatgga
     8641 tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt aggtaaacca
     8701 atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac tcctaaatat
     8761 ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga aaacatgagc
     8821 ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa agtacgcgtc
     8881 atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc tacttttaca
     8941 actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg attcttgatc
     9001 ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat gatgacgatg
     9061 tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct atcgggaatg
     9121 ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat ttagctatcc
     9181 tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg aacttgcgag
     9241 agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat ttgctttttg
     9301 tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa cgcatcaagt
     9361 ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact tctacaacca
     9421 ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag acgtcgattg
     9481 gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa tcagagctga
     9541 ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg attacgaaag
     9601 gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa tgagtgtaga
     9661 agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg ttcaacgagt
     9721 ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac gacaaaacac
     9781 accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc aagaacatgc
     9841 taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg gctatgacaa
     9901 agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac gtccaaaagg
     9961 tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca ttgctgacga
    10021 agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc aattctgaat
    10081 agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga atcagctcaa
    10141 acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta tgaagatttt
    10201 gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg ggtgtcaacc
    10261 atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg gaatggttgg
    10321 caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa gcggattatc
    10381 aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat ggcaacagta
    10441 ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct aaaagaatta
    10501 gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt aggcgcaact
    10561 tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg ggtttcgtcg
    10621 ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag cgaaatcatt
    10681 ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc gccttacgtg
    10741 tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg attgacaaaa
    10801 ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg ggtagaattg
    10861 caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt atacttaaca
    10921 gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa taacaaagag
    10981 ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca aaagaaccag
    11041 aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt tgtaaaaaca
    11101 aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa aaacattcaa
    11161 acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg agaaagcgga
    11221 aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta cagcagatat
    11281 tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg cgagcgagtt
    11341 agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca atgaaaaaaa
    11401 gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat ggatcaaatc
    11461 tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc cgactatcat
    11521 tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga tggtgctgat
    11581 ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg aggtaaataa
    11641 tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta gatagcacta
    11701 gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag tcgaatatgc
    11761 gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt agtcaaaaga
    11821 cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa ttaacaaagc
    11881 gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct aaccttaaca
    11941 cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg aacgacacaa
    12001 aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa atcaaagaaa
    12061 atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat gaatctggtg
    12121 cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag aaagctatcg
    12181 aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca gcaaatgaaa
    12241 ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata tctagcaggc
    12301 aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa gattttggcg
    12361 acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta agctcgctaa
    12421 aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt agcagtgtag
    12481 taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca ttgatgaagt
    12541 tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc tcgaaaaaag
    12601 aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg aacacaaccg
    12661 cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg gcaggtaact
    12721 tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg atgctaacgc
    12781 agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca gatgcgatac
    12841 cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat acaggaaact
    12901 tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa gcaattgtac
    12961 agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca ttgagcggtt
    13021 cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata gaaaaaatag
    13081 gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa aattctattc
    13141 ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc gttgaaaata
    13201 gagagccact aacatggatt gtcggaatca taggcggaat tacattagca gtaaaagcat
    13261 tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc ccttttgtgg
    13321 cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat acaaaatctg
    13381 aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta gttatgagcg
    13441 ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag tggctttggg
    13501 aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg aagaacagcg
    13561 ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc aatggcgttg
    13621 gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa gcaactgaat
    13681 tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa gacacattcc
    13741 gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag atgaagaatg
    13801 ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc aacgcagcac
    13861 ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg gtactagaaa
    13921 aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca ggtacaccag
    13981 caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga gcagaaacag
    14041 ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt ctaaacgcac
    14101 caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt tctaaagctc
    14161 ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat agtgtgaaaa
    14221 aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg tgggacttta
    14281 tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta ttaggtggat
    14341 tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca agtgcactga
    14401 ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta ggaacaaatg
    14461 gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc ccaggatttg
    14521 tggcaactag tgggtataga cctatagacc aggtgaccct tattcacatg gtaaacgtaa
    14581 tgccattgat attgcattac caggcgtcac aggaggctca cctagataca cagaagcagc
    14641 aaactacgct tttgacaaat tcgcttccaa aattggttac gtaatcacta atgggaaagt
    14701 tcgtgaccgt tcaggacaat caggtcaacc agcaactggt gcatgggagc catggcccga
    14761 tggagatcac tatgatcatg tgcatttaaa cggtgtgaaa gacccacaaa acactcaaat
    14821 ttcaggagat agcgtgggag gcagtggggt agaaagatgg cgcaatgtag caattagagc
    14881 gttgaaaatg accggtcaat acagtactgc aaacttaaat gcattactaa atcaaatgcg
    14941 tacagagtca aatggtaatc ctaatgcagt taacaattgg gatattaacg ccaaaaatgg
    15001 aacaccatca aaagggttgc tccaagtgat tgacccaaca ttcagacagt atgcaatgcc
    15061 aggattcaac agcaatattt ttgacccact atctaacatc ttagcttcaa tcagatacgc
    15121 actatcaaga tatggctcac taacaaatgc ctatcgtgga gttggttacg caaacggtgg
    15181 aattgtaaac caacatcaaa ttgcggaaat cgcagaagga aacaagccag aaattattat
    15241 tccgttagat aaggctaaac gatcaagagc gatgcaatta ttgtctatcg caatggacaa
    15301 actaggagtt tctcctaaaa cttatggaaa tgcgacaact gtttccagtg attacgaaat
    15361 cctagaagcc attgaaaaac aggcggcaac aacgaatcag ctgttatcgt tattgctggc
    15421 atttttcaaa ggaaatagcc gaactgatag agatttagct ttagatattc agaagatctt
    15481 ggttaggagg atgtagatgc gaactgtatt actgagaaat agaataaacg aagaaattga
    15541 tttgtccaca gaagactatt ttgctactgg gttgagtaat atggggtttg aagtaaaaaa
    15601 agaacatgtg ggacagtggg ggaatttcag agaaagcagt gagagcgttg aaatatctga
    15661 atttcagtca tctgtaatca tttctgtgca tgggtttcga gaaaaagaac tgtacaattc
    15721 acttgtgcag ttcctatcgg aaggtccgtt tgaactggaa tttgcttttg acggcgaaac
    15781 aatggtaaga agatgcagtc taaaatcttt aagtaagact gaaatcgatc cgaaaacatc
    15841 gttactaaca gacactttag aactatattt cacatcaaac tggtattccg taaaacgcga
    15901 aaaattaatt cagcgaccga atgtagtgaa aacacgtggt aaggtttttc cgtataaaag
    15961 atcttatatc tacactcaaa acttgtggga gaaaaaaggt gtattcaaat tcaataataa
    16021 ttctgtatac ctaacgaata gcaaggaacg gatgtccccg ctaaaaattc gtgtgattgg
    16081 gaagtgttca aatccgtatt gggaagtaat ccaaaactca caaatcatcg caacggatgg
    16141 atacttcata gatatgactg aaacacagac tctagaagta tcaagccttt ttgaagatac
    16201 aacggcgatt ttaaaagata ttgcaggggt agaatcttct gtctaccaac aacaggatta
    16261 tacaaaaact aattttgttc aggccccaac tggagaattc agcattgtgt ttcatgttgg
    16321 gggggcagac gtagagattg agttatacga ggagcgtgat ctgttttgat tttagcagtc
    16381 acgctttttc atcgtgattt aatgctttat aacgaacatt tattctctaa cagttttgaa
    16441 tttggtgtgg acgagatcaa cgaagaggct agtagcttta cgatggataa gtacgttcct
    16501 gtaaaaacag gcgattttct actagcaaaa tatattccta gtggaaaatt tgcatatttt
    16561 ggtgtaatta cgtcgcagga agacgaaaaa atcagttgta aaagcttact tagtttagct
    16621 gatagtgaga taccgactgc acgtgtgtca ggagataact acgaagagca tatccggcgg
    16681 ttgattgaat actatttgct gaatgatcca acgaaacaac taaaagatat tttagacgtc
    16741 aaagcagaaa gtgcgacctc tcattcgtat caagctaccg atacgaataa acacaagtta
    16801 agtgcgtata ttctcaatgg tttcaaaaaa tacaacgtaa aatggtattt caagggaatt
    16861 caaaacagaa aaatttatac aggcatacgt gctgtaaatg aatcaattta cattaaagac
    16921 aattcttctg aatttagcga ttgggatgtg tttgttcagg cgccgggcgc tggaaacgag
    16981 aacaagctat taatcgttga taaagcaatg aaagatatag agaaaccgat aatactgtca
    17041 acatggtatt tggacgaaga aaacaatttg acacaagatg gatcgaaaga aaatatcacg
    17101 aaaccaactg tcaatttggt taatatctac gatcaaaccg cagaagataa agcatcctac
    17161 gaagatgtgg caaaatcaga gttgaaaggc aatacgtatt cgcatgaaat caaagtgaac
    17221 gttgtaagaa atgcaaaaaa tttgaatgtc gaaacgattg aaacagggat gtttgccacg
    17281 atttcttata aaggaaagat atacaagtcg gttttgacag cttggcgaat atcaagtgat
    17341 aaggagtttg tggaattgac tttcggaaac ataagaagtc gttttatgga ttattttgaa
    17401 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    17461 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    17521 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    17581 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    17641 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    17701 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    17761 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    17821 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    17881 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    17941 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    18001 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    18061 aaggagcgtg taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    18121 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    18181 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    18241 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    18301 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    18361 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    18421 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    18481 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    18541 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    18601 acttcatcat agacgtgtta gaacgagtgg atattaattc ggagcaagcg aaatactata
    18661 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    18721 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    18781 atgaatcaga taagcaagta aatgatcgta ttgatcaaac caatcagcaa atcggcgatc
    18841 tcggcaagct gaaaaagatg tacagtaaca gcatcgactt tgggggctat gattattcgg
    18901 ggaatccgaa tttgttaagt aagctatcat acgacttaat tgaaaatcaa aatacttcag
    18961 ctggaacact ttctaagggt gaaaactcgt ttaaatataa taagatatca gctgaagtgg
    19021 aaggtggagt agagttatat tataaacgaa gaggtatagc taactggtta ccctctaata
    19081 aaacgcttgt aatgactgtt aagcttagag ctggagcgga ctatagtcca gttgacggaa
    19141 aacttatact gattagatat aggtatgttg acagtggaac tggcaagatt gttttagact
    19201 tacctattaa cagtaattcg ataactcagg aatggaaaga gtttagtatt actggaacta
    19261 ctccaacatt tagcccacaa gcataccatc cttggataca atttagggct caagatgggg
    19321 tacttgggga aatagaaatg agctatgaca tcaaaatcga agaaggctct acagctacac
    19381 catatcagcc taacttactt gatgactctt actggctagg taaaacaccg ttgggtgaga
    19441 atttgataac gaatgccaag ttcccaatta taacacgaag caatcctatt tctggttttg
    19501 atatttcaga agaattaata ataggtgaaa cttatacggt atctttaaaa gggactaagc
    19561 cagctactaa agaattccac ttatactacg gagaagcaaa ttaccagcag ttgcagtatc
    19621 aggcaacttt acttcctgtg gaaggattaa cagatgtgtg gagtgccact tttacagcca
    19681 gaaatactag tgaaacgaca aatttactta gagtagcgtt atggcaaaaa cctaattctg
    19741 caacatatga tactgttcaa attgactggc tcaagattga aaaaggcgac acacgcacac
    19801 caaatatcag tcagtttaaa tactttggtg aaggcttgaa agacagtaac aatccgaacg
    19861 actacagctg ggatgtcaca ccagagtata ctgaaaaaag cttgaataat acggttagtc
    19921 tgactgaacc gcaatccatc gaaggattga aaaatttcga agatggtatt caatcaaaag
    19981 gaaaatctgt attgacatca gacgacaaca aatatgaagt cgtaacctta acagttacaa
    20041 acgggaatac cggatcagca aagctttatc gtgaaggaaa aaccgtcact atttattttt
    20101 ttgcgttgaa tgggaaaagc agtggtggaa atgattcaac gatactaaca attccagaag
    20161 gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt tctactttga
    20221 acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga aactcaagtt
    20281 atggatcgga ttataccttt gcaattactt acacgattta gaaaggcgtg aatcgatatg
    20341 aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt gaatgatgaa
    20401 gaacatttac cggaagaatg cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa
    20461 ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggccgt actggcggag
    20521 agtatgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca gatcagccac
    20581 cgctcgaagg ggttcgttca gatggcgaac cgctaacgat tgctgatcag atgtttgatc
    20641 ctaaattgaa ccaatggatt attttagcga acgcgttaga tcacaacgat ttaaacaatc
    20701 tcaaagcgat gtacgaggct cttgaacatg aaaacggcaa cctaaaacag ctaaatgcca
    20761 aactcatgct aagcgatgta gcgattaaac aggaaaatac tgcattgaaa gaaaaagctg
    20821 acagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca
    20881 tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt
    20941 tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca agttcgtcta
    21001 ttcgttccac tatgtatcac agatgaagaa gcagatagaa ttattagcaa agaagagagc
    21061 gcatcttaag tgatgcgttt ttttgttgga aagttggtgg aacatgaaag aagaagcgct
    21121 ccaagacgtt gtggagagat tagtaagaat tgaaacaaaa ttagacaact acgaatcact
    21181 tagagaaaaa gctgatagtg caaaagattt ggcagacaaa gcctactcag tagcgctaaa
    21241 caacgcagaa gatatcaaag aaatgaaagc caataataaa tggtcgtggg gttacatgat
    21301 cggtttaggc attacgatca ttggctattt cttgactaaa ttgtaaagga gttaagaaga
    21361 aatgatttta cccgataagt attatcaagt cattaaatgg acggttttaa cagtattacc
    21421 agctgcatct gttttagtag ccacgttagg aaaagcgtat ggatggaatg gaacagatat
    21481 gacagtactc actatcaatg cagtagcaac atttttaggc gttatcactg gtgtgtctgc
    21541 atataactta aaaaaatagg aggaaaaaaa tgaagaagaa aatcatttta tcattgagcc
    21601 taataatggc tcttttttta ttgccaatta acgggttcgc ctatacgatc aacaatgaat
    21661 ttaatttagg tgtaaatgaa ggtagctc
//
LOCUS       DACDEA010000042        21359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDEA010000042 DACDEA010000000
VERSION     DACDEA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.042"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IV136_001684"
     CDS             474..725
                     /locus_tag="IV136_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465675.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IV136_001685"
     CDS             759..1037
                     /locus_tag="IV136_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465676.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IV136_001686"
     CDS             1634..1891
                     /locus_tag="IV136_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465677.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IV136_001687"
     CDS             2004..2615
                     /locus_tag="IV136_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465678.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IV136_001688"
     CDS             2794..3351
                     /locus_tag="IV136_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465679.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IV136_001689"
     CDS             complement(4050..4571)
                     /locus_tag="IV136_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8465680.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IV136_001690"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IV136_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8465681.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IV136_001691"
     CDS             8651..9538
                     /locus_tag="IV136_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8465682.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IV136_001692"
     CDS             9535..10704
                     /locus_tag="IV136_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465683.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IV136_001693"
     CDS             10728..12083
                     /locus_tag="IV136_001693"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8465684.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IV136_001694"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IV136_001694"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8465685.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14370..14660
                     /locus_tag="IV136_001695"
     CDS             14370..14660
                     /locus_tag="IV136_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465686.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14673..15698
                     /gene="sppA"
                     /locus_tag="IV136_001696"
     CDS             14673..15698
                     /gene="sppA"
                     /locus_tag="IV136_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8465687.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15701..16360
                     /locus_tag="IV136_001697"
     CDS             15701..16360
                     /locus_tag="IV136_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8465688.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16517..17449
                     /locus_tag="IV136_001698"
     CDS             16517..17449
                     /locus_tag="IV136_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465689.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17603..18892
                     /locus_tag="IV136_001699"
     CDS             17603..18892
                     /locus_tag="IV136_001699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8465690.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19139..20389
                     /gene="clpX"
                     /locus_tag="IV136_001700"
     CDS             19139..20389
                     /gene="clpX"
                     /locus_tag="IV136_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8465691.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20472..21059
                     /locus_tag="IV136_001701"
     CDS             20472..21059
                     /locus_tag="IV136_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8465692.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7085 a         3642 c         4605 g         6027 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaagagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgagta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccaa
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc gccacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagaactt
    12901 ggcggctatc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacaggg atctgtgtca
    13021 atttttccag agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagaatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccattgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccagcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 tattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctcctcttt tgttttttat ctggaaacat ttaaaatcat aaccttttgc gaatgttctt
    14221 gtcaacaaaa aaagaataaa aaataaattg gtatatatca aatgctttgt caaatgctct
    14281 ttttatgtca aaaaataatg ggtttttatc tatcatcttt ttcaggagtt cggtacaata
    14341 gaaagtaatc ggatagagga ggagtaaaaa tggattcaaa aattgagatc atgacactgg
    14401 gaatgttaaa aaaacaattg tccaaattcg aagcatcagc tggtgtatca gatgatacga
    14461 aaatattttt agatactggc tgggatagca tccaagaaat cgcacctgat gccttggaag
    14521 ttgctgaagc tcgtgagttt acggtagaag atgaattgac aaaagagtcg ttttccgggt
    14581 atgcgcggga agaaaaagca gagcggttcg acacttctga gcagtcagaa actgtgatcg
    14641 tcataaaaaa cttgtattag aaaggagaaa gaatgaataa gagacgttgg gcagcagttc
    14701 tgattgctgc aggattattt gtggtttcgt tagtttcagc tagtttgact aagcctcagg
    14761 aaaacgatca aattgaggga ttgaacagtt ggttatatgg ggatgaagaa ctagcttcaa
    14821 atgtgttgga agagggagac agtacgcaaa aaatcgtaaa attatcagta gatggaacga
    14881 ttgctgcggg agggtcttca ggtttatttg cttctgaagg ttacaatcat aaaaatttta
    14941 tggaacaact gaaggcgatt gaagaagatc caacagtcaa aggtatcttt ttagaagtaa
    15001 attctcctgg tggcggtgtc tacgaaagtg cggaaatcgc taagaaattg gatacgattc
    15061 gtaaagaaca tgatatccca atgtatgtca gcatgaaaaa catggcagct agcggtggct
    15121 actatatttc tgctcaagca gataaaatct ttgcaacaga agaaacagtg accggctcaa
    15181 ttggtgtgat catgtcagga ttgaattatt caggacttct agaaaaactg ggtgtggaag
    15241 atacaacagt taaaagcgga gcgttgaaag atatgggttc aggaacgcga ccagagacaa
    15301 aagaagaaga agcggtgctt caagcatata tcgacagcgc atatagtcgt tttgtagcaa
    15361 tcgtcagcga gggacggaat aaatctgaag aagaagtcaa aaaaattgct gatggaagga
    15421 tttatgatgg tgtccaagca aaagaagtag gtttagtcga tgaattggga tttcctgaag
    15481 atgctttaaa agctatgcga aaggaacaac agcttgaaga tgcggaacta gtagaatatt
    15541 cttccacgcc atctgggttt gctagtacat ggttaggaag taaactagca gaattccagg
    15601 gattgaagcc ttctgagact agtcagatct tatccgttct tgagaatcta gggacagcgg
    15661 aatcaccaaa agcaatgtat tattacggag gtgagtaagc atgtcagagc tttcaaatga
    15721 aaataaggaa caagaaaaag ataagcaaca gcagcctgct gtaggacctg actccaactt
    15781 ttcccaaaag gtactgagct cttttgaaag agagcctttg acgcccgaag aaatcaaaaa
    15841 gaaacaacgt aaatggaaaa agtatacgga cacaagaaat gaaccgccaa aagcaaatga
    15901 ttttcctaag tatttctatg caggtttttt tatccgaatg tttgctttct tggtcgatct
    15961 tttgtgtatc ggagctatta cgcgtttaac acttggtatt gccaaaaatc taggttggct
    16021 taatttttca gattcttatc tgagtatcta cggattcaca gccttactta tttatctagc
    16081 gtattttatt ttattgacga aattcaatca tggtcaaacg attgggaaaa tgatctttgg
    16141 tattcgagtc atcagctttg atgaagaaga acttagctgg tcaaccgttt tagttagaga
    16201 aggtgcttgc cggttcattc taaaatttcc tttgctgatg ttagggtatc ttccagcaat
    16261 ctttaatcag aaaaagcaac atctaggcga ttttttcact aatacaagtg ttgttacgat
    16321 caatctgatc aaagcattca accaggaagt caacagctga tgagcaataa aaagcagtaa
    16381 cagttgaaag caccttctaa aagtcagatc taaaatctag cttttagagg gtgcttcagt
    16441 tatttttaat tgtcatgctt tttttgtttt gctactattg aacatgagct gaacatgagc
    16501 aagaggagga cactgcgtga agccgattga ttttccagaa aaatatgaga atttaatgcg
    16561 agtagcacaa caagcactag caaaccagca atatcagcag gcaaaagaat tatttcaacg
    16621 agcttacgag ttgaaagaaa gctttgaggc taatagcctg cttgtttttt gtctttacga
    16681 gttggacgag aaaaaagaag cattgaagca agcactcttg catgaaaaac agtatcttca
    16741 aaatgaggag tttgctgaat tttattttga cttgttaatc agtacacaag attttttata
    16801 tgctagaaaa ttgatagcaa gtacagattt ttatgaagct ttcgagcagc gggtcattga
    16861 aaaaatccaa tttgcagaag aattatccgg acaaatggag aggcagaaga tcaaggcact
    16921 ccacaataaa tcggaggaac ttccttcgtt ggaacctgca cgtcagcttg cgtctattga
    16981 tgagatcgaa cagcttcctt atcatgagtt tatccaaaca gcgagtaaac ttattgtatc
    17041 gccagaggtc catattttag caagggcaaa attactggag acacttaggc aattgaacga
    17101 atgccatcct gttttctatt tgacgattga agaaaagtta gtcaaagtca taccaaaaga
    17161 tctgccaaag ccgcagcagc aggcaagtta ccagcaatta tgtgtatttt cagatcgtta
    17221 cggtaatgaa gatgcgctgc ttagttccgt gttaaatgaa gaattcacgt tgcaatctgc
    17281 aattgtttat cctgtctatg atacatacat cgaagacccg aagcgctggt ttcaactgac
    17341 tgtagaggcg tgtacaggaa aaacgatgta tgaaggtaca gaagatgaaa aacaagattt
    17401 tttaaaaaaa agagaaaaaa ttttgcagca aatgatcttc ttccattaga ttttatcagt
    17461 gaaaaagata aaaatgttat gaaaaacaag gattctatcc attcaatatg tttacaattt
    17521 gaaagggtta gtgtactatt taaaggtatg catgaaacgt ttttgatgta taaaatgaat
    17581 ttctaaactc ggagggaatt aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg
    17641 tgttttaaaa tttacgattg cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa
    17701 taaagtgaaa aaaagcttga acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt
    17761 attcaaccgt atgtacggtg aagaagcttt atatgaagat gcattgaatg ctgtattacc
    17821 agaagcttat gaagctgctg tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat
    17881 cgatgttgaa agcatggaaa aaggtcaaga ctgggtaatc actgctgaag taacagtgaa
    17941 accagaagta aaattaggac aatataaaga tctagttgtt gaaaaacaag accgtgaagt
    18001 aacagatgct gacgtggatg cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt
    18061 tatcaaagaa gatgctgcag ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt
    18121 tgtagatggc gaagcgtttg atggcggaaa aggcgaaaat tactcacttg aattaggttc
    18181 aggctcattc attccaggtt ttgaagatca attagttggt cataaatcag gtgaaagtct
    18241 agatgtaaat gtaactttcc cagaagatta tcaagcagaa gatcttgctg gaaaagaagc
    18301 agtcttcaaa gtgacaatcc atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga
    18361 attcgcaaaa gatgttgatg acgaagtatc aacattagac gaattgaaag aaaaatatcg
    18421 caaagaatta gtagaaacaa aagaaaaagc agcggacgat gcaaaagatg aagcagcgat
    18481 ccgtatggct gttgaaaacg cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga
    18541 agtacatcgt tcaatggacg aattcttgaa caacatgcaa cgtcaaggta tctctcctga
    18601 aatgtactat caattaactg gttctacaga agaagacttg cataaacaat tcgaaggtga
    18661 agcagaaatg cgcacaagaa caaatcttgt gatcgaagca attgcagctg ctgaaaatct
    18721 tcaagcaaca gaagaagaaa tcaatgcaga aatccaagaa ttatcagaaa catacaacat
    18781 gccaatcgaa caaatcaaac gcgtgttgac agaagatatg ctggaacatg atgtgacaat
    18841 gaaaaaagca atcgaattga tcacaggtac agcgacagaa gcgaaagaat agaaatcaag
    18901 taataaatca tttctgaaga agctacttct agaaaattgt ttattcgaaa taagggactg
    18961 tgacacgatt ttgtcacagt tccttatttt ttgttaagag taaaaaatgc aaaggtatag
    19021 gtcagttgat cctttgttaa acactttttc tttttactaa actatgttat gatagtaaaa
    19081 gactgtaaag tctttgatag tttcttgcat actttttgca agttaagggg tgaagaccat
    19141 gtatgacaat ccaagtagca atgagaccgt acgctgttct ttttgcggga aaacccaaga
    19201 agaagtgaaa aagatcgtag ctggtccagg ggtctacatc tgtaatgaat gtatcgacct
    19261 atgtaaagaa atcattgatg aagaatttta tgaagaagct gtacgcgaat tcactgaagt
    19321 ccctaaacca ctagaaattc ttgaagtatt gaataactac gtgattggac aagatcgcgc
    19381 aaaacgctcg ttagctgttg ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca
    19441 agaaaacaca gatgatgttg aactgcaaaa aagcaatatc tgcttgatcg gacctacagg
    19501 gtctggtaaa acatttttag cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat
    19561 cgcagatgcg acaagtttga cagaagctgg ttatgtgggg gaagatgtag aaaacatttt
    19621 gcttaaattg cttcaatctg ctgattacaa tgtggaacgt gcagaaaaag ggatcatcta
    19681 tattgatgag atcgataaaa ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt
    19741 ttccggcgaa ggtgtccaac aagccttgct gaaaattcta gaaggaactg ttgcaagcgt
    19801 gccacctcag ggaggacgta aacatccaca tcaagaattt attcagattg atacgacgaa
    19861 catattgttc atcgtaggtg gtgcatttga tggcatcgaa acgattgtga agaatcgtat
    19921 gggcgaaaag acgattggat ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt
    19981 catgcagcat atcattcctg aagatttact aaaattcgga ttgatccctg agtttatcgg
    20041 acgattacct gttatggcag ctttagagaa attgacaaca gatgatctgg tacggatctt
    20101 gactgagccg aaaaatgcgc tagtcaaaca atatcaaaaa cttctttctc tagataatac
    20161 cgaacttgaa ttcgaacctg aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa
    20221 taccggtgct cgaggacttc gttccatcat tgaagaaatc atgatggatg tgatgtttga
    20281 tatcccttca aatgaaaaca tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc
    20341 tggtgagcca acagtaatct ataaagattc agagaaaaaa gcgggttgat gaaaagaggc
    20401 tgccgtaaaa gtcattttgg cttttgcgac agccgcttgc tttattcatc accttgttaa
    20461 ggagagagac catgaaagtc catcaagctg aaattatcat aagtgcagtg gcaccaaaac
    20521 aatatccaga tacacaattg ccagaaatag ctctggcagg acgatcaaat gtagggaaat
    20581 cttcttttat caacacattg attgatcgaa agaatttagc ccgtacttcc ggtaagccag
    20641 gaaagaccca gacattgaat ttttatttga tcgagaacgc attacatttt gtcgatgttc
    20701 cgggatatgg gtatgcaaaa gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg
    20761 agacatattt aacgactaga gaccagctac gtgcggtcgt ttctttagta gatttgcgtc
    20821 ataagccttc tcaagaagat atccagatgt atcaattttt gaagtattat gatatccctg
    20881 taattgttgt tgccactaag gcagataaga tccctcgtgg taaatggaac aagcatgaga
    20941 gcgaaatcaa aaaagcgttg gattttgatc gcaatgacac gtttattctc ttttcttccg
    21001 ttacaaaaca gggaaaagaa gaagcgtggg cagcaatcga acaagcaatt gaatcataaa
    21061 aagaataaaa aagacttacc agtcaaactc tttcataaaa gtttggctgg taagtctttt
    21121 tttgagctta acttactttt gtttcttgtc ttttttcttt tcattttgtg gctgtggttt
    21181 tagaaatcga tcgatctctt cttttggtgc aactgttttt tccggatcga cagcaaatga
    21241 ttcgaaaagt ttatctaaat tatctgtacg ttcaaatttc aaccccattg taaacacctc
    21301 tttgtttctt tttacttagt ctatcatgta atcgtccaaa ttggtttcta agacataaa
//
LOCUS       DACDEA010000043        20112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDEA010000043 DACDEA010000000
VERSION     DACDEA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.043"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IV136_001702"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IV136_001702"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8465693.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IV136_001703"
     CDS             complement(1028..1948)
                     /locus_tag="IV136_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8465694.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IV136_001704"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IV136_001704"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8465695.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3677..4210
                     /locus_tag="IV136_001705"
     CDS             3677..4210
                     /locus_tag="IV136_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8465696.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4700..5224)
                     /locus_tag="IV136_001706"
     CDS             complement(4700..5224)
                     /locus_tag="IV136_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465697.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV136_001707"
     CDS             complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV136_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8465698.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV136_001708"
     CDS             complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV136_001708"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8465699.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6946..7626)
                     /locus_tag="IV136_001709"
     CDS             complement(6946..7626)
                     /locus_tag="IV136_001709"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8465700.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7752..8621
                     /locus_tag="IV136_001710"
     CDS             7752..8621
                     /locus_tag="IV136_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8465701.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8647..9162
                     /locus_tag="IV136_001711"
     CDS             8647..9162
                     /locus_tag="IV136_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465702.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9209..9772)
                     /locus_tag="IV136_001712"
     CDS             complement(9209..9772)
                     /locus_tag="IV136_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465703.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9827..10006)
                     /locus_tag="IV136_001713"
     CDS             complement(9827..10006)
                     /locus_tag="IV136_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465704.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10083..10592)
                     /locus_tag="IV136_001714"
     CDS             complement(10083..10592)
                     /locus_tag="IV136_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465705.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10688..11068)
                     /locus_tag="IV136_001715"
     CDS             complement(10688..11068)
                     /locus_tag="IV136_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8465706.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11398..12147
                     /locus_tag="IV136_001716"
     CDS             11398..12147
                     /locus_tag="IV136_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8465707.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12331..12498)
                     /locus_tag="IV136_001717"
     CDS             complement(12331..12498)
                     /locus_tag="IV136_001717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465708.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(12514..13608)
                     /locus_tag="IV136_001718"
     CDS             complement(12514..13608)
                     /locus_tag="IV136_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465709.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IV136_001719"
     CDS             complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IV136_001719"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8465710.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14530..15474)
                     /locus_tag="IV136_001720"
     CDS             complement(14530..15474)
                     /locus_tag="IV136_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8465711.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15476..16162)
                     /locus_tag="IV136_001721"
     CDS             complement(15476..16162)
                     /locus_tag="IV136_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8465712.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16635..16955
                     /locus_tag="IV136_001722"
     CDS             16635..16955
                     /locus_tag="IV136_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8465713.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            17116..18021
                     /locus_tag="IV136_001723"
     CDS             17116..18021
                     /locus_tag="IV136_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8465714.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18018..18791
                     /locus_tag="IV136_001724"
     CDS             18018..18791
                     /locus_tag="IV136_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8465715.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(18968..19879)
                     /locus_tag="IV136_001725"
                     /pseudo
     CDS             complement(18968..19879)
                     /locus_tag="IV136_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         5912 a         4096 c         3586 g         6518 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     3061 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3121 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     3181 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3241 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     3301 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     3361 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     3421 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     3481 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     3541 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     3601 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     3661 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     3721 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     3781 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     3841 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     3901 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     3961 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     4021 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     4081 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     4141 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     4201 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     4261 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     4321 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     4381 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     4441 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     4501 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     4561 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     4621 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     4681 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     4741 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     4801 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     4861 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     4921 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     4981 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     5041 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     5101 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     5161 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     5221 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     5281 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     5341 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     5401 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     5461 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     5521 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     5581 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     5641 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     5701 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     5761 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     5821 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     5881 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     5941 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     6001 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     6061 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     6121 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     6181 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     6241 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     6301 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     6361 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     6421 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     6481 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     6541 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     6601 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     6661 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     6721 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     6781 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     6841 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     6901 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     6961 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     7021 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     7081 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     7141 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     7201 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     7261 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     7321 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     7381 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     7441 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     7501 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     7561 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     7621 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     7681 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     7741 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     7801 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     7861 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     7921 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     7981 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     8041 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     8101 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     8161 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     8221 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     8281 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     8341 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     8401 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     8461 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     8521 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     8581 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     8641 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     8701 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     8761 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     8821 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     8881 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     8941 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     9001 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     9061 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     9121 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     9181 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
     9241 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
     9301 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
     9361 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
     9421 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
     9481 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
     9541 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
     9601 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
     9661 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
     9721 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
     9781 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
     9841 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
     9901 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
     9961 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    10021 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    10081 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    10141 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    10201 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    10261 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    10321 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    10381 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    10441 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    10501 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    10561 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    10621 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    10681 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    10741 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    10801 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    10861 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    10921 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    10981 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    11041 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    11101 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    11161 ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg
    11221 ttcccaatat aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta
    11281 tcataacaca aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata
    11341 gattgtgtta gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg
    11401 aagtcatatt ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat
    11461 cttttctttt tcagtcgtgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg
    11521 ctgtccgtta ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat
    11581 gcggcaaaat cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt
    11641 gggttttcag caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg
    11701 gatatccccg tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa
    11761 ttaactggaa aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca
    11821 ggattcctga ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt
    11881 tctattagct tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg
    11941 tggttagctt cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct
    12001 gcaagtttgg cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt
    12061 accgtttcac cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa
    12121 tactacaacg cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac
    12181 ttttgtctca cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat
    12241 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
    12301 gttaaacgct tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc
    12361 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    12421 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    12481 agattggata tctttcacta ctagttgcca tttttatttt gagactgtac ggtctttctt
    12541 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    12601 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    12661 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    12721 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    12781 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    12841 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    12901 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    12961 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    13021 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    13081 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    13141 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    13201 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    13261 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    13321 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    13381 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    13441 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    13501 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    13561 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    13621 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    13681 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    13741 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    13801 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    13861 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    13921 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    13981 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    14041 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    14101 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    14161 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    14221 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    14281 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    14341 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    14401 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    14461 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    14521 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    14581 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    14641 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    14701 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    14761 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    14821 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    14881 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    14941 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    15001 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    15061 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    15121 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    15181 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    15241 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    15301 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    15361 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    15421 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    15481 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    15541 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    15601 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    15661 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    15721 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    15781 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    15841 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    15901 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    15961 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    16021 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    16081 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    16141 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta
    16201 taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat
    16261 atttttcgtt atttttttat tatataattc accttactta ttgtaagata aagctttttt
    16321 taatttattg cctgctaata tataaaaata aggtctagtt actgtttttt taaatgatta
    16381 ataaataaca actcctttct atgaaattgc aaagcattca tcttcttttc tgctaacgat
    16441 atatccgtca atgcttgtta cactttacta aaaagtatga gattgccgat atctggatag
    16501 gtgtgctata ctacagaaaa agatatacga tctgccaccg ggtagtaaaa aagaggatga
    16561 tcattccatt aggtctttga agggatgatt ctcctctttt tgtgtatttt ttatattgaa
    16621 ggaggaacgt atgtatggca tggatggaat taatcatagc aggaattttg gaagtattct
    16681 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
    16741 tgattggaat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgcctatga
    16801 gtctctctta tcctatttgg acagggattg gcgcagttgg ttcggtcatt attggtgtgg
    16861 tcttgtttca tgataagctg aatctttcca cttggttctt tgtcgggttg ctgctcatca
    16921 gcatcattgg catcaaaatc actagcggtc actagatact tagacaagca gacaaggact
    16981 gatcagaaaa agcggggctt tttatttgat attcctcttt ctatgaaaaa acaagaaaat
    17041 aagactaatg acctagcgac ttctctactc atcaggtgta tgcttgtact atctatagaa
    17101 aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac
    17161 aaacaagccc tcagcgatgt cagttttagc ttatctcctg gcaaaatcgt tggattagta
    17221 ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac
    17281 tcagaaggaa cgatccgtat tggtgaaaat gagatttctc cctctagtca taaaggatta
    17341 gaacaagtag gagcattgat cgaatatccg ggtatctatc cattcttaac aggttatgat
    17401 catctgaagt tattctctga aacaaacgat gtatctgcta tcgacactat cgtgaaacag
    17461 ctcaagatgg aaaaatacat tcacaaaaaa gcgaaatcct actcgctggg aatgaaacaa
    17521 aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg
    17581 atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct
    17641 actcagggca cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc
    17701 gatgagctga tcctgatcaa tcaaggaaaa atcatcaaac aatgtacaat ggctgaattg
    17761 aaccaaacag cgcaagattt catcgttatc tctacgacaa atgatcaagc tgctcgaacg
    17821 atcttgacag acgctggata tcccttggaa gatcctgata aaatcaagct tctaaaaaaa
    17881 aacgaagagt tattaggaac tgttatccag ttattgtctg cagaaaatgt ccatgtgaca
    17941 gatgtccagc atctgacgaa tgatttggaa tcttccctat tggaaattct ggattcagta
    18001 aagggggaag aagaaaaatg acgttgctta taaaacaaga attatttaaa ttgatcaaga
    18061 aaaagtcaac tgctgtttta tctgttctat tagttgcttt gctcatagga acagcacttc
    18121 tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag cttttcagtg
    18181 ccacatcttg gattgtcttt atcatgattg ctgctgcaag tacgatcatt tcaatggaat
    18241 cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag
    18301 taagtaaatg gatcacatta gttatttatt ctatttattt atacctttta gctattattg
    18361 tcaccgtgct gatgaaactg atccttttcc cttcgatttc ctttacggag aagatttcta
    18421 ctggtcagac actgatccaa agcttattca cttatacttt gggtagttat atcggtttat
    18481 ggctaatttt aagtttagtg ttgatgattg cttgttttat caatagttcc ggtgcctcca
    18541 tctcagcagg gatcgtcttt tattttgctt catcaatcat atctggtatc ctcatcgcac
    18601 tcattcaaaa atgggagtgg atcaaatgga atccaatcag tatgctgaat ctgcaaaacc
    18661 aaattggcaa cgaagagatt atgaagcaat tgactcatct ttctactaat caaatgctgt
    18721 ttggcaattt agcttacatt gctttgttct tagcattggg atacctcgta ttcaagcgaa
    18781 aaaatgtatg atggttcatt aacaaagaag ttgtgacaga agtggccagt ttcaagaaat
    18841 ttcggcttat ttttagaaga agctacttct tgaacaccat ttattcaaaa aaagaagcga
    18901 tgacacgaat gttgtcaccg cttctttttt gaataaacca caggcttcac agcctctctt
    18961 tatacttcta tacgtgaagc atcccagtgg caaataaaac cattgcgatt actccgacca
    19021 tcgtcacacc gataagtaaa atcttatcta agtggatatg ttgtacaggt tcgttttgtt
    19081 ctttacgtgc tttgaggtaa aaataaatac ctggtaagaa aagaacaaga acaagcatca
    19141 cttcattaaa ggctacaact agcatggccc atacttgaaa agcacttgct aaaaacccta
    19201 ctaaaaattg gacccactct ttccgctgat aagaaagctt catttggtac agccctaaga
    19261 aaagccacgt gaagaaaatc gctgctgtac caagtgtata agcaaagtta tatgcactgt
    19321 cagtgaataa taatgaaagt aaaaagacat tcgtacataa cgtcgtcaat acgatagcat
    19381 aagttggtgc cccatttttg ttgagttttc cccatgcttt aggcaaaagt ccgtcctcag
    19441 ccatattccc cattgtttca atcggcaaga ttgtccatga gagccaaacc ccaactaaag
    19501 aaatgatcaa accgccgttg atcacgaccg ccccccattt accaaccatc atttcaaata
    19561 catatcccat agcaggttgc gctttgatcg tttgcagctg attcatcgtc attgtgccat
    19621 atggaagcat ggaaaccaag acgtaaataa tgatcaaacc gaatattcct agaatcgttg
    19681 ctttcccaac atccgattgt ttttttgctc gtgaacctac gacagaagct ccttcaatac
    19741 caacgaatac ccagagcatc accatgatgc aactcaatac ttgactagaa aacgggatcg
    19801 gctgcccatt cgcattcaat atgatatcgc tagaaaagtt attaatgaat tgttggatcg
    19861 aaaaggtgct gatagctacg taagcgcccc atagaacagt accttactaa caaatgcacc
    19921 tcaagatata cttgagctat caagaagtct ggaggttttc tatgtataaa ttaaatgaga
    19981 ttgttcctgt ccaattagga aacgaaaaga gtgcaccaga aaaaggtaaa cccgcggctg
    20041 ccaagttagc gatgcgcatc aaaagagaga atggttctga agttttgatc tataacggta
    20101 ttaacaatta ta
//
LOCUS       DACDEA010000044        20094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDEA010000044 DACDEA010000000
VERSION     DACDEA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.044"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IV136_001726"
     CDS             72..506
                     /locus_tag="IV136_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465716.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IV136_001727"
     CDS             complement(584..1117)
                     /locus_tag="IV136_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8465717.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV136_001728"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV136_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8465718.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV136_001729"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV136_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8465719.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IV136_001730"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IV136_001730"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8465720.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IV136_001731"
     CDS             complement(4842..5420)
                     /locus_tag="IV136_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8465721.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IV136_001732"
     CDS             5742..6212
                     /locus_tag="IV136_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8465722.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IV136_001733"
     CDS             complement(6320..7546)
                     /locus_tag="IV136_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8465723.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IV136_001734"
     CDS             complement(8300..8500)
                     /locus_tag="IV136_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8465724.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IV136_001735"
     CDS             complement(8634..9242)
                     /locus_tag="IV136_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8465725.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IV136_001736"
     CDS             complement(9327..9797)
                     /locus_tag="IV136_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8465726.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="IV136_001737"
     CDS             complement(9845..10759)
                     /locus_tag="IV136_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8465727.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="IV136_001738"
     CDS             10905..11708
                     /locus_tag="IV136_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8465728.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="IV136_001739"
     CDS             11886..12833
                     /locus_tag="IV136_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8465729.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="IV136_001740"
     CDS             complement(12901..13194)
                     /locus_tag="IV136_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8465730.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="IV136_001741"
     CDS             complement(13222..13887)
                     /locus_tag="IV136_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8465731.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="IV136_001742"
     CDS             complement(14027..14659)
                     /locus_tag="IV136_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465732.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="IV136_001743"
     CDS             complement(14666..15733)
                     /locus_tag="IV136_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8465733.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="IV136_001744"
     CDS             complement(15730..16461)
                     /locus_tag="IV136_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8465734.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV136_001745"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV136_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8465735.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV136_001746"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV136_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8465736.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="IV136_001747"
     CDS             18624..19793
                     /locus_tag="IV136_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8465737.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         6075 a         4020 c         3230 g         6769 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaacc ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc
    20041 taaaatatgt gctagcaaat cattcatggc ttgttcttat ttttaatttt atgc
//
LOCUS       DACDEA010000045        19770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDEA010000045 DACDEA010000000
VERSION     DACDEA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.045"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..791
                     /locus_tag="IV136_001748"
     CDS             144..791
                     /locus_tag="IV136_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8465738.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            875..1819
                     /locus_tag="IV136_001749"
     CDS             875..1819
                     /locus_tag="IV136_001749"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8465739.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1954..3405
                     /locus_tag="IV136_001750"
     CDS             1954..3405
                     /locus_tag="IV136_001750"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8465740.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3426..4262
                     /locus_tag="IV136_001751"
     CDS             3426..4262
                     /locus_tag="IV136_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8465741.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4262..4921
                     /locus_tag="IV136_001752"
     CDS             4262..4921
                     /locus_tag="IV136_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8465742.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5047..5736
                     /locus_tag="IV136_001753"
     CDS             5047..5736
                     /locus_tag="IV136_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465743.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5742..6698
                     /locus_tag="IV136_001754"
     CDS             5742..6698
                     /locus_tag="IV136_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8465744.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6742..7254
                     /locus_tag="IV136_001755"
     CDS             6742..7254
                     /locus_tag="IV136_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8465745.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7510..8058)
                     /locus_tag="IV136_001756"
     CDS             complement(7510..8058)
                     /locus_tag="IV136_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8465746.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8147..9328)
                     /locus_tag="IV136_001757"
     CDS             complement(8147..9328)
                     /locus_tag="IV136_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8465747.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9564..11072)
                     /locus_tag="IV136_001758"
     CDS             complement(9564..11072)
                     /locus_tag="IV136_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8465748.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11231..11422
                     /locus_tag="IV136_001759"
     CDS             11231..11422
                     /locus_tag="IV136_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465749.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11625..12542
                     /locus_tag="IV136_001760"
     CDS             11625..12542
                     /locus_tag="IV136_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8465750.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12535..13608
                     /locus_tag="IV136_001761"
     CDS             12535..13608
                     /locus_tag="IV136_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465751.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13756..14274
                     /locus_tag="IV136_001762"
     CDS             13756..14274
                     /locus_tag="IV136_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465752.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14255..14647
                     /locus_tag="IV136_001763"
     CDS             14255..14647
                     /locus_tag="IV136_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8465753.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14723..15658
                     /locus_tag="IV136_001764"
     CDS             14723..15658
                     /locus_tag="IV136_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8465754.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15791..16570
                     /locus_tag="IV136_001765"
     CDS             15791..16570
                     /locus_tag="IV136_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8465755.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16563..16880
                     /locus_tag="IV136_001766"
     CDS             16563..16880
                     /locus_tag="IV136_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8465756.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17124..18734
                     /locus_tag="IV136_001767"
     CDS             17124..18734
                     /locus_tag="IV136_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8465757.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18891..19739
                     /locus_tag="IV136_001768"
     CDS             18891..19739
                     /locus_tag="IV136_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8465758.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6385 a         3527 c         3970 g         5888 t
ORIGIN      
        1 aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc
       61 tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt
      121 ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag
      181 ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt
      241 ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt
      301 cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg
      361 cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg
      421 aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag
      481 aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc
      541 tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat
      601 tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa
      661 cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg
      721 gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac
      781 aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac
      841 tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa
      901 tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga
      961 attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa
     1021 tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc
     1081 tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa
     1141 agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga
     1201 aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa
     1261 tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta
     1321 tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc
     1381 ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac
     1441 ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc
     1501 agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac
     1561 tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg
     1621 tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat
     1681 gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta gaatcataaa
     1741 agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa
     1801 attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat
     1861 aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg
     1921 tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa
     1981 gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata
     2041 aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc
     2101 cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta
     2161 aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa
     2221 acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt
     2281 ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc
     2341 tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga
     2401 gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt
     2461 tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg
     2521 atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg
     2581 acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt
     2641 gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag
     2701 atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct
     2761 gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac
     2821 ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca
     2881 gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa
     2941 cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat
     3001 tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat
     3061 ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt
     3121 gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac
     3181 gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat
     3241 ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta
     3301 ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta
     3361 tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa
     3421 gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg
     3481 cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga
     3541 aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa
     3601 atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca
     3661 aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat
     3721 cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt
     3781 cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa
     3841 tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata
     3901 tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc
     3961 agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc
     4021 agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt
     4081 tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa
     4141 tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat
     4201 attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt
     4261 gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt tcgtactgca
     4321 aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa atctggtagg
     4381 gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt
     4441 ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt tatattttgg
     4501 gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag
     4561 tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga
     4621 cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca
     4681 agcttttcga atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac
     4741 ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg
     4801 tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg
     4861 tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc agtcaatctg
     4921 aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt cttgtatatt
     4981 ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg ttcggaggta
     5041 gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca atattgcatc
     5101 gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg aattgtagat
     5161 acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta tgagctgaaa
     5221 gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca ttataattat
     5281 tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac aggaataggt
     5341 gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg tacatttctg
     5401 attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc
     5461 ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa
     5521 tattttccaa cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca
     5581 ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca ggcaatcaat
     5641 gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag gcaacaacag
     5701 cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat ccttatttaa
     5761 gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga ttgctctcca
     5821 ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg
     5881 aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa gtaggacaat
     5941 tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc
     6001 ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct ccagcgtttc
     6061 ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag ttggcaggag
     6121 ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg cccgaaaaca
     6181 aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat gcagataaaa
     6241 cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat attggatttt
     6301 ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc cgagcgcttg
     6361 ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat tttgtctctg
     6421 acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga gataaaatga
     6481 aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg attgaattat
     6541 tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag tggtcactta
     6601 tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg cttaaatatt
     6661 attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta aatgaaaaag
     6721 gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa
     6781 gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg
     6841 caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag aaaagaaagc
     6901 tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc aagcgtggat
     6961 cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta tttatcgatt
     7021 catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa
     7081 catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga tacttatcca
     7141 gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca agggatgatt
     7201 gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa
     7261 aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca tatgatagcc
     7321 aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc gaaaatgctt
     7381 ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg aaactccctt
     7441 catcgagagg tcgtgacata gcttttgtca cgacctctcg gcttatccta cgagtattat
     7501 tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt ggaacgctcc
     7561 atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact gtgctaaatc
     7621 ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac gttttttcag
     7681 gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta ccgtcccgaa
     7741 atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt
     7801 cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat attggtccca
     7861 ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa caaaataata
     7921 atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg
     7981 ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag gaccgataaa
     8041 tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga aaaagctttg
     8101 gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtatattat accagcaagc
     8161 cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt
     8221 gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca gaaatgatcc
     8281 cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct gttgtacgct
     8341 cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca tttgtaacca
     8401 gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt ccgctgatga
     8461 aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg aatatcccgt
     8521 ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata gcagcaaccg
     8581 tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa aatgtctgtt
     8641 ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag ggatttacaa
     8701 ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc acatatttag
     8761 aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac atagccgata
     8821 cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt atggaaataa
     8881 aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc aatttgccca
     8941 tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga agaatcttga
     9001 tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat acggaaagaa
     9061 agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca ccttgcccag
     9121 caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct gtaccaaaag
     9181 ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca attagtggtt
     9241 tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat gtgacaaata
     9301 aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt ccgttttatt
     9361 ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact tttttagagt
     9421 gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt
     9481 aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt
     9541 caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt
     9601 gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc
     9661 acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg
     9721 attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca
     9781 tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt
     9841 gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact
     9901 gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat
     9961 tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca
    10021 tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata
    10081 atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct
    10141 gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc
    10201 acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata aacagattct
    10261 ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta
    10321 tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct
    10381 tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc
    10441 catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga
    10501 tacagttggt tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca
    10561 acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt
    10621 ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt
    10681 ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg
    10741 acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata
    10801 tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg
    10861 caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct
    10921 tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct
    10981 aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata
    11041 tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat
    11101 tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga
    11161 aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg
    11221 aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc
    11281 gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac
    11341 ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac
    11401 aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta
    11461 tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt
    11521 ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa
    11581 gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata
    11641 caaatgcaaa ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac
    11701 aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat
    11761 tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc
    11821 gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga
    11881 tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg
    11941 aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg
    12001 atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg
    12061 atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg
    12121 agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa
    12181 aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa
    12241 aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat
    12301 taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga
    12361 agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa
    12421 tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga
    12481 attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat
    12541 gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg
    12601 aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg
    12661 ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca
    12721 gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa
    12781 gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt
    12841 atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt
    12901 atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg
    12961 tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac
    13021 acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc
    13081 ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta
    13141 aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc
    13201 cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa
    13261 ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta
    13321 gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta
    13381 ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac
    13441 gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa
    13501 aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc
    13561 tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg
    13621 gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat
    13681 aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga
    13741 gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct
    13801 tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt
    13861 tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct
    13921 attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc
    13981 tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat
    14041 ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc
    14101 gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg
    14161 tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc
    14221 gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct
    14281 tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc
    14341 ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa
    14401 tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat
    14461 tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca
    14521 ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac
    14581 tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa
    14641 actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag
    14701 ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat
    14761 tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag
    14821 tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat
    14881 tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg
    14941 tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg
    15001 tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg
    15061 taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt
    15121 acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt
    15181 tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt
    15241 aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc
    15301 atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct
    15361 agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa
    15421 aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca
    15481 tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa
    15541 tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa
    15601 gaagatgtat atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa
    15661 cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg
    15721 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
    15781 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
    15841 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
    15901 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
    15961 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
    16021 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
    16081 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
    16141 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
    16201 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
    16261 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
    16321 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
    16381 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
    16441 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
    16501 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
    16561 ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
    16621 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
    16681 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
    16741 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
    16801 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
    16861 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
    16921 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
    16981 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
    17041 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
    17101 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
    17161 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
    17221 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
    17281 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
    17341 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
    17401 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
    17461 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
    17521 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
    17581 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
    17641 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
    17701 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
    17761 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
    17821 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
    17881 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
    17941 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
    18001 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
    18061 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
    18121 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
    18181 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
    18241 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
    18301 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
    18361 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
    18421 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
    18481 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
    18541 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
    18601 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
    18661 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
    18721 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
    18781 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
    18841 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
    18901 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
    18961 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
    19021 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
    19081 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
    19141 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
    19201 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
    19261 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
    19321 tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt gtagatgatt
    19381 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
    19441 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
    19501 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
    19561 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
    19621 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
    19681 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
    19741 tccttttatt caaaaaacat accaaaaata
//
LOCUS       DACDEA010000046        19754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDEA010000046 DACDEA010000000
VERSION     DACDEA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.046"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            149..1600
                     /locus_tag="IV136_001769"
     CDS             149..1600
                     /locus_tag="IV136_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8465759.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="IV136_001770"
     CDS             1949..2581
                     /locus_tag="IV136_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8465760.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="IV136_001771"
     CDS             3169..4608
                     /locus_tag="IV136_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8465761.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="IV136_001772"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="IV136_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5370..5585
                     /locus_tag="IV136_001773"
     CDS             5370..5585
                     /locus_tag="IV136_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465762.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="IV136_001774"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="IV136_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6245..7156)
                     /locus_tag="IV136_001775"
     CDS             complement(6245..7156)
                     /locus_tag="IV136_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465763.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="IV136_001776"
     CDS             complement(7175..8179)
                     /locus_tag="IV136_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8465764.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="IV136_001777"
     CDS             complement(8176..10299)
                     /locus_tag="IV136_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8465765.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="IV136_001778"
     CDS             complement(10304..12751)
                     /locus_tag="IV136_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8465766.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="IV136_001779"
     CDS             complement(12738..13127)
                     /locus_tag="IV136_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465767.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="IV136_001780"
     CDS             complement(13187..13690)
                     /locus_tag="IV136_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8465768.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..13927)
                     /locus_tag="IV136_001781"
     CDS             complement(13703..13927)
                     /locus_tag="IV136_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465769.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="IV136_001782"
     CDS             complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="IV136_001782"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8465770.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16687..16806)
                     /locus_tag="IV136_001783"
     CDS             complement(16687..16806)
                     /locus_tag="IV136_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8465771.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16821..18005)
                     /locus_tag="IV136_001784"
     CDS             complement(16821..18005)
                     /locus_tag="IV136_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8465772.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18264..18554)
                     /locus_tag="IV136_001785"
     CDS             complement(18264..18554)
                     /locus_tag="IV136_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465773.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="IV136_001786"
                     /pseudo
     CDS             complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="IV136_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         5838 a         4031 c         3502 g         6383 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc
    13921 ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc
    13981 gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag
    14041 tttaattctt gattgccagc taatactcta tatttattta gtcttatgag ctggacttta
    14101 tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga
    14161 cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca
    14221 gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata
    14281 gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg
    14341 cgattttcca aagataattt cattaactgg tacatcagta atttatcaac acttagattg
    14401 gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt
    14461 ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac
    14521 cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt
    14581 tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct
    14641 ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata
    14701 cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga
    14761 gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca
    14821 cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca
    14881 agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc
    14941 aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc
    15001 ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac
    15061 tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata
    15121 tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct
    15181 aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct
    15241 ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt
    15301 aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg
    15361 gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg
    15421 ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta
    15481 aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata
    15541 cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt
    15601 ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca
    15661 acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt
    15721 attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc
    15781 agtaatatat tttctctcga taggataata tccataagat gagtgaaaat atctccattc
    15841 ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt
    15901 aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact
    15961 tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt
    16021 tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt
    16081 cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt
    16141 gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat atatccttag
    16201 gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa
    16261 cctatattct tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag
    16321 ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat
    16381 agatatttta tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat
    16441 agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa
    16501 cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt
    16561 acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt
    16621 ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc
    16681 atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa
    16741 gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat
    16801 tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
    16861 aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt
    16921 gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct
    16981 agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct
    17041 ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt
    17101 ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc
    17161 tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt
    17221 cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat
    17281 tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat
    17341 aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt
    17401 ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt
    17461 atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg
    17521 gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt
    17581 tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga
    17641 gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa
    17701 tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca
    17761 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga
    17821 ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca
    17881 ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt
    17941 tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt
    18001 gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac
    18061 aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt
    18121 gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca
    18181 tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta
    18241 acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc caactctgct
    18301 ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca
    18361 aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat
    18421 ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt
    18481 tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac
    18541 tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg
    18601 aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc
    18661 tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt
    18721 ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac
    18781 actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact
    18841 gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc
    18901 accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg
    18961 ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat
    19021 tttgattcct tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc
    19081 acccattcct cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt
    19141 tgaagacggt ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat
    19201 aaggaatatc cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc
    19261 cacgtcatac cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc
    19321 gtgattgagc aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt
    19381 gaaccgagcg atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc
    19441 cgcaattgaa aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga
    19501 ttgaataaac tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc
    19561 atgaattgct ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg
    19621 atggctcgcc tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa
    19681 tctcatacat tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc
    19741 atacattatt aatt
//
LOCUS       DACDEA010000047        19669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDEA010000047 DACDEA010000000
VERSION     DACDEA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.047"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(191..421)
                     /locus_tag="IV136_001787"
     CDS             complement(191..421)
                     /locus_tag="IV136_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465774.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1038..1700
                     /locus_tag="IV136_001788"
     CDS             1038..1700
                     /locus_tag="IV136_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465775.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1779..5006
                     /gene="fss3"
                     /locus_tag="IV136_001789"
     CDS             1779..5006
                     /gene="fss3"
                     /locus_tag="IV136_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8465776.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5120..5434
                     /locus_tag="IV136_001790"
     CDS             5120..5434
                     /locus_tag="IV136_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8465777.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5447..5821
                     /locus_tag="IV136_001791"
     CDS             5447..5821
                     /locus_tag="IV136_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8465778.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5831..6175
                     /locus_tag="IV136_001792"
     CDS             5831..6175
                     /locus_tag="IV136_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8465779.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6246..7598
                     /locus_tag="IV136_001793"
     CDS             6246..7598
                     /locus_tag="IV136_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8465780.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7658..8800
                     /locus_tag="IV136_001794"
     CDS             7658..8800
                     /locus_tag="IV136_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8465781.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8825..9079
                     /locus_tag="IV136_001795"
     CDS             8825..9079
                     /locus_tag="IV136_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465782.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9335..10519
                     /locus_tag="IV136_001796"
     CDS             9335..10519
                     /locus_tag="IV136_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8465783.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10534..10653
                     /locus_tag="IV136_001797"
     CDS             10534..10653
                     /locus_tag="IV136_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8465784.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            10658..10879
                     /locus_tag="IV136_001798"
     CDS             10658..10879
                     /locus_tag="IV136_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465785.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10892..11392
                     /locus_tag="IV136_001799"
     CDS             10892..11392
                     /locus_tag="IV136_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8465786.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11489..13336
                     /locus_tag="IV136_001800"
     CDS             11489..13336
                     /locus_tag="IV136_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8465787.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13339..14235
                     /locus_tag="IV136_001801"
     CDS             13339..14235
                     /locus_tag="IV136_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8465788.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14285..14674
                     /locus_tag="IV136_001802"
     CDS             14285..14674
                     /locus_tag="IV136_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465789.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14661..16163
                     /locus_tag="IV136_001803"
                     /pseudo
     CDS             14661..16163
                     /locus_tag="IV136_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            16908..18734
                     /gene="ltrA"
                     /locus_tag="IV136_001804"
     CDS             16908..18734
                     /gene="ltrA"
                     /locus_tag="IV136_001804"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8465790.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18868..>19669
                     /locus_tag="IV136_001805"
     CDS             18868..>19669
                     /locus_tag="IV136_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8465791.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6642 a         3215 c         4306 g         5506 t
ORIGIN      
        1 ttcttcattc tagtttttgc agcagggatt ttaaagtagt tcttattaga atagtagaga
       61 ttgattaata agctatcttc gattgctaga aagaaaactt ctgaatccca caccttataa
      121 aaatctttga taaatctatt cgaaggatca aatttaaacc ataattgtgt ctcattaaaa
      181 agcatagcca ttactccgat ctatttttaa acttagtttc tacctctaat atatatcgag
      241 tttttatttt gccttcagat aatactgttt cttttttcgc aattacaggt tggttatttt
      301 cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct tttttgctgc
      361 tttgctcata aaagtctgca tattcatcac tgatattttt tctaataaag tcttccatca
      421 taaaaatcac ctcaaaacga ttatacgaac ttacgttctc gttgtaaagc gatatttgag
      481 gggcaaaaag ggggcaaaaa tacgtttacc tttgatatat ctcagattga acatagtgtt
      541 tattatatgt cggtattgtt gatttatagc tgttttgagg gtattagata cataatagaa
      601 tatagtggtt ttgtttacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatcagaa
      661 gataaacacg gaaaacttcc ttggaaatct gaaatttgtg aaaagtcctt gtatatcaag
      721 ggctttttct tttttctcta tcacagtttt aagccatttt tggctgtttt ggaccatttt
      781 tcaatcatat tcccttaaaa attgtcctag attgtccgac attgactatc agtgtcgtca
      841 aaatagggca aaatttagta gtcagcttga cgacaaatat aatacgaaaa ataccaaaac
      901 atttaccgaa tttagcaact tttgaaccca agtttctcaa agggtcagct attagcaaaa
      961 aagacaaaac atgtactttt ctactaaaaa gttgatgtga taggcttcta tctaaataac
     1021 tattaggagt ttttatcgtg aagcaaaatg atggacgtat catttggaaa aatcaaagtg
     1081 agttaaaact gattttaaca attaatgaat tcatcgaaaa acacggaatt acatctagcc
     1141 gtcaatacca gaaaaagcta tcagaaaatc caaattctgc accaagtatg tggttcatta
     1201 acaaaaaata tggatcatgg gagaatttat tgattagtat aggacgagaa aatactgggt
     1261 atggaaaatg ggctagaatg tcagaacaag aattattgga aatagtagaa gcctttatta
     1321 agtgcgagaa gataacctct caacgaatgt acgaacaaaa atcagtaggg aagaatattc
     1381 cttcacttag taccattaaa aagatgttag gagatatacg tccacttttt aaagaaaaaa
     1441 atgatggatc tcgatttact gatttcgaac tgctgttaga actaaaaaat gaaataatcc
     1501 gattaaaact acaggatgat ttatcaatga caaagtttcg aaaactagtt caatccccaa
     1561 aactaccatc agttgataca attatgaaaa gaacgaacaa aaattgggag gaattgatgg
     1621 cagagattgg atttgattat cgaagaatca aaatttataa gcagagaaac aacctatcca
     1681 agacaaagaa aactaaatag tatcactcta aatcagacgt tcaaaattaa aatttggacg
     1741 tcttttttat tacccaaaaa cgaaaggaga tttattttat gaaaaagcca agatttaaga
     1801 attggcgttt gctcgcaaca ctgagtctat tatgccaaac aattggtgga tcgcttggcc
     1861 caacgattgc ctttgctgat gaaattactc atccacaaac ggtaactgtg gaattggatt
     1921 tggctcacca gtatgctgtc gaaggtacat ttagcgatgg tcgtcccatg tcagaagtta
     1981 ccgttccgca ctatgcagtt tacaatggtg tgaagcaaga tattttctgt attgagccag
     2041 gggtgccgat ttacaatgag ttcactcctg gttatgagaa aaatccattg cctgatatgt
     2101 cggagaaagc gaaattagtt tccgttttat ggaaggaagc tggtacggat gtggatacac
     2161 atattgtggc ccagaagatg atttggcaag aagttaatgg ctacacgctt cactcaatta
     2221 aacgttctga tggtagtgca gtaaatatcg cagccattga gtcaaaaatc aatcaagcca
     2281 ttgcggatta tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc gtgttaggtc
     2341 aatcgactac tgtgacagat acgaacggct tgaatttatc ggagtttgat gaagttgtcg
     2401 agaatatcgc aaatattgat tatcgtgtga acggcaatca actagtgatt actccaaatg
     2461 ccaattcaaa agagagtggt gaattaaccc taaagaagtc agctaataca ggaacaccgg
     2521 tagcttataa aatggctggt caacaaactt tgatggcggg ggcaattgat aagcccaata
     2581 cctacacaat caaaattgat gtggaaactg agggttcttt gaagattaaa aaagtcgata
     2641 aagaaacagg aaatgttgta ccgggaaccg ttttccattt agactttgga aaaacattac
     2701 ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac attggatgag attcctcatg
     2761 gcgcgaaagt aaccattact gaaaaatcag tgccagctcc ttatacgatt gacactacac
     2821 caatcaccgc aaccattaaa tctggtgaga ctatttctgt gacttcaaaa aatactcgcg
     2881 aaaaaggtca aatcattttg aataaaatgg gggtggaaac aggaactgat ctttggaacg
     2941 acaaatactc attagccgga aatacgtttg ccattcgcaa agacagtcca accggtgaaa
     3001 ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa gagattgcca
     3061 atgcgttgga attgggaacc tattatgtga ccgaaaccaa agctagtaat ggctttgtga
     3121 atacctttaa accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg gctcttgtta
     3181 ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga aaccactttg acaaaagaag
     3241 acaaagatac cggtaatgaa actcaaggta aagctgagtt taaaggagct gaatatactc
     3301 tctttactgc aaaagatggt caagcggtca aatggagtga ggcttttaaa ccagagttag
     3361 tgaagggaac gaaagcttct gatgaaacgg tgactttgac tttagatgaa aagaatcaag
     3421 ttgctgttaa acacttagcg attaatgagt attactggca agaaaccaaa gcacctgaag
     3481 gatatacctt ggatgaaaca aagtaccctg tttccatcaa aaaggtggat gataacgaaa
     3541 aaaatgccgt gattacccga gatgttacgg caaaagaaca agtcattcgc tttggctttg
     3601 atttctttaa atttgctgga tcagctgctg ggactgccga aactggcttc aatgatctga
     3661 cctttaaagt atcgccattg gaaggaacca gtgagattac agatgccgaa gatgaagcaa
     3721 ttacagctta taacgagcaa ctaggttttg atggctatgg caagtttgaa aatcttcctt
     3781 atggggatta tttacttgaa gaagtagagg ctccagaagg atttcaaaaa attacgccat
     3841 tagaaatccg ttctactttt aaagaaaata aagcggatta cactaagagt gaatacattt
     3901 ttacgattac cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt ccttacgaga
     3961 aactgacgaa taaagcattt tctgttagct tgaatcgttt gatgctctat gatttacctg
     4021 aagaagaaga tagcttaact tctcttggga cttggaaaga cggaggcaaa gaattgacag
     4081 accttgattc taccgagcta gttgataaat tgagctataa cttgcatgaa atcaaagaag
     4141 actggtatgt ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc caagaaaaag
     4201 atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc aacgttggcg aataaagaga
     4261 aaacaggaac ttggaaaatt ttgcataaat taaccgcaga acaagttttg gataaaacca
     4321 tcgtcttatt caactatgtg tatgaaaaca agaaacccta tgaagcaggt gatgatccag
     4381 tagcgaaaga tgctagcttg aacaatcaag cccaaactgt caattgtacg gtggaacgcc
     4441 atgtttccat ccaaacaaaa gcccacctag aagatggttc gcaaaccttt actcatgggg
     4501 atgtggtgga tatgttcgat gatgtgtcga ttacccatga tgtactggat ggctcaaaag
     4561 aagcttttga aacaattctg tatgcattac ttccggataa tacgaccaaa gaaatttgga
     4621 aatctggcaa aattgattat gaagtgaatg acaaagaatt cactaaaacc gtacttgcgg
     4681 aaaaagtgga cacaagtaaa tatccagaag gtacaagttt taccttcaaa gaaattaact
     4741 atggcaaaga cggaaacatc aatgggaaac acaatgagga tctaaaagaa aaaactcaaa
     4801 ctttaacacc taaaaaagtt ccgacaacac caagtgcacc agaacaaccg gaaataccaa
     4861 ctgttccaag tgactctcaa gaatctagtc ctacagtaaa gacattccca caaacagggg
     4921 agaaaaattc taatgttctg ctgtttattg ggtttacctt gatctttgcg actgctgggt
     4981 attatttctg gaatcgccga aactaaggtg atgtgatgaa acaactaaat aggaaaaacc
     5041 aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc aaaactaaaa
     5101 atgaaaagag gaaaagaaga tggaattgaa atttgttgtg ccagatatgg ccgaaacctt
     5161 tggaaagata aggtatgccg gcgaaggcga agttttaact gaaggatatg ggcggaatac
     5221 tacagtaatt ggtcgtagtt atcatttgta ttccagtaag caacgagccg atgatattga
     5281 agtggtggta gctgcggaag ccggcgaaaa ggattttgat caagatcaac cactaaaagc
     5341 cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt ttaccgacta
     5401 cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga
     5461 aggtattgtt gtagattcag gactaacgtt tgggaaatta cgattttccg cattacgccg
     5521 agaagtacgg aagcaaaatg aggatggaac ggttagtaac gaggtaaaag aacggactta
     5581 taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt agtatcccag caaatgtacc
     5641 cttacgcgag tttgcctatg acgcagaggt agagttggtc aatccaatca tggatacggt
     5701 cgccaattat gttttccgag aaggaactac tgtcaattgg ttcattaagg ccgatgattt
     5761 agtcttgaaa cgacaaccag atcagggaaa ttctacaaac caaaatgaag gaaagaaata
     5821 ggtgaagcgt atggaaatta atgttgaaca tattttagat tgtctcgatc aatatggaaa
     5881 aggtgagcta acggaggaac aactcactaa agcattaacc tatgatgaaa aaatgtttct
     5941 gattatgcat caagggttac tggaagtagg taacgatgcc gaagaagatt ttgatgtact
     6001 gaattggtta ggggaagaag agtccttctt tatggttatc gaagtaaatg aaagtctatg
     6061 tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa atgccggatg ccattgaagt
     6121 ttttttaaag caactagtgg aaacgaaaca actccctata gcagtaaatg actagcataa
     6181 ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt tcgattggag gtggaaacca
     6241 taaaaatgta caaagggcat cgtatacggg caggagatca gcacttggtt tatcactttg
     6301 ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat tttcaagagc
     6361 ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt cgttctataa
     6421 aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg ttgctttata
     6481 tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta gcccggatga
     6541 ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt ttcaaagacc
     6601 tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat taccgtatga
     6661 aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa gatcagcttt
     6721 taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa aaggaactca
     6781 aagactccta tgtggagtac actttactgt atgatataat tgccaatcga attgggatag
     6841 acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta tgggcttatg
     6901 attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact tactttttac
     6961 tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt gatcccaaaa
     7021 atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa aaggaggaaa
     7081 tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag gcaatgaaag
     7141 aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca ccaaactttt
     7201 taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa agtgcggtga
     7261 ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc tttctgattc
     7321 tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat caatttaatt
     7381 tctgggtggc tttgggtcgt atgagtgaac tgggatattc catgatgttt ggcgaggttg
     7441 ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta tgtagatacg ggagggagtg
     7501 tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta agggaaatta
     7561 gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg tagcctagga
     7621 gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag atttattcgc
     7681 tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta tcggcttttg
     7741 tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa gtaaggaggt
     7801 ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg gaccagtgga
     7861 tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaggatt
     7921 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta ttctccaacc
     7981 taagttttta ttccttgaga acgtccgggg attactcaat cacgaaggag gggctacgtt
     8041 cgagacgatc ctccgaacgc tggatggatt ggggtatgat gtggaatggc aagtgcttaa
     8101 ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc cttatcggac attctcgaga
     8161 cgcatgtacc gaacaagtat ttcctatcat tggatcgtct ccaacatctg atcaaaacat
     8221 cagaaactta ttaaatatca acccttccaa tcgaggaatg gggggacaag tctatgggtc
     8281 agatgacgta gcgcctacct tgaccggtga tgaaggaatc aaaatcgcat tgccggtaga
     8341 tgatggaatt tctgtagcgg gtatgttact gggaaatttt gaacaaggca atcgggttta
     8401 tgagataact ggaacagcgc ctactttgtc aacgaaacaa ggcggaacga agatcatgct
     8461 tcggaaaaac gaaacagaat atcaagaggt gaagccaggg aacagtgtga acctggcttt
     8521 tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa agtgtgcata ccttattaac
     8581 aggagaccaa caagcagtcg tcacggatca gtatcaaatt cgaaagttaa cacctaggga
     8641 gtgctggcgg cttcagggct ttccggactg ggcttttgat cgagcttctc aagtcaattc
     8701 agatagtcag ttgtataagc aggctggaaa ttctgtaaca gttccggtca tttttgatat
     8761 tgctagaaga ttaaaggagg taaaaacaga tgatttatga tgagttgatt ggggaatcta
     8821 ctgggtgatt ggcaagattc agtcagatcc ggaattggaa gaagaactgt accggctcaa
     8881 ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat ccgtatgtaa ttgatgagga
     8941 aacggatgct cgaatggagc tgaatcaagt catcagtgaa ttggaacgaa ttgcggactt
     9001 tgctaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt tggaaattga
     9061 gggtattacc gcggagtaac ggccaaataa accactgata ggcactgacc gcgggggatt
     9121 tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg gacggcgtta gccggccatg
     9181 atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaataa gcaagcaacg
     9241 ataaggaatg gctgtcctat caaggataat aagcaatttg tgtagatgtt aactgatttg
     9301 aaaaagttaa caccttaaat ttggaggtga gaacttggca caaaggaatt tagattatcg
     9361 gctattaaaa gaccggcgta atgaatatgg cgtttcacaa aataaattag ctacgacttg
     9421 tggacttagt cgcccgtact taaatcagat agaaaatggt ggcgtaaccg catcgacaaa
     9481 aaccatgagg aaaatttttg accaactgga aagtttcaat cccgagttac ctttaacgtt
     9541 actgtttgac tatgtaagga ttcgctttcc cacaacggat gcgaggaaaa tcattcaaga
     9601 gattctccat ttgaaatttg attatatgct ccacgaagat tacgcctttt actcctatca
     9661 agaacaatat gttatgggag atattgtggt gatgttgtca catgaagaag ataaaggcgt
     9721 ccttttagaa ttaaagggtc gtggttgtcg gcagtttgaa acttttttac tcgcccaaaa
     9781 gcgtagctgg tatgactttt tcgaagattg tctaaaagcc ggtggcgtga tgaaacgctt
     9841 agacctggca atcaatgatc gagttggcct gttagatatt cctgatttaa cgaaaaaatg
     9901 tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agctatcgtt ccggggaact
     9961 gttgaaacct gatgataaag atggtatggg caatactttg tatattggca gtcttaagag
    10021 tgaggtctat ttctgtttgt acgaaaaaga ctatgagcaa tatatcaagt tagggattcc
    10081 tttagaccag accgaaacga aaaatcgttt tgaaatccga ctgaaaaatg atcgagctta
    10141 tcatgcaatt caagatttgt taaaaggtcg cagtattgaa agtaccacat tttccattat
    10201 taaccggtac ttgcgatttg cggataaggt ggaaggcaaa aggcgaacta actggccctt
    10261 gaatgaacaa tgggggcgct tcattggacg gaatcagaag gaaattcaat taacttctga
    10321 accgaagcct tataccattg aacgaacgtt gaactggctt ggacgacaag tcgcccctac
    10381 gtggaagatg gccaaggagt tggatcggtt gaatcagaca acgtacattc aggatatggt
    10441 aaataatact cgactatcag accggcacaa aaagattttg gagcaacaaa gtatggcaat
    10501 tgaaaatttg attatatgaa aagaggaatc gtgatggaaa ttgtgaaaga tattttattg
    10561 gtgtttggtg gcagtatgct tggtgttact gtgatgtgct tgatgcatgc aagtgcagcg
    10621 gcggaccgag caatggaaaa gaaaggaaag tgatgaaatg aatttcggtc aaaatttgta
    10681 tcaatggttt ttaaccaatg ctcaatcttt agtcttacta gcgattgtgg tcattggttt
    10741 gtttttagga tttaaacgcg agttttccaa actgattggc ttcttggtga ttgctttgat
    10801 tgcggtaggt ctggtcttta atgcttctgg tgtgaaggat gtcttactca atttgtttaa
    10861 ccgaattgtc ggtgcctgag ggggaatcac aatggaacag atacgtgttt atattgcgaa
    10921 ccttggaaaa tacaatgaag gtgagttggt gggggcatgg tttaccccac ccgtcgattt
    10981 tgatgaagtg aaagaacgga ttggattaaa tgacgaatat gaagagtacg caattcatga
    11041 ttatgaactg ccttttgaga ttgatgagta tacaccaatt gaggaaatta atcggttgtg
    11101 tggtttagcg gaagagctta aaggaacgcc aattggtgag gtcgcttcag agattcaaca
    11161 tgccttcttc aattctttcg aggaaatggt agagcatgtg gatgatatca tctattatcc
    11221 cgattgtaat gacatgagtg atgtagcgta ttacttgatt gtagaagctg gtgatttagg
    11281 cgaagtacca gaacacttga agacgtatat tgattttgat gcgtgtggtc gagacttgga
    11341 gattggcggg aattatttgg ttacgagtcg aggggttttt gaactaatgt aaatcatttg
    11401 ttagaattga ccagaaattt ctatcgtact tttacttttc tatttgatat actgaaatag
    11461 aaaatatttg ttgatagggg tgttactaat ggttggcaat aagtggtaca aatgtgatct
    11521 tcatattcat tcaactgcga gtcagtgctt tcaggataaa aaggttactg ctaaggaatg
    11581 ggtagaagaa tgtttaaaca aaggactcga ttgtgttgca ttaactgatc acaatacagg
    11641 ggaaaatatt gatgaatata agaaagtggc tatcgaaaat ggcttagatt tttttcctgg
    11701 agtggaatta acgtgtggag aaaatggaac tcatttacta gtcctatttg ataaaagtgc
    11761 atgtacaaat gatatctctg atttcttaac caagatgggt ataaaaagag aggattgggc
    11821 cagcgctgca gcttgctcaa tgaaatcagt tttagaagtt attgattttg ctgagcacga
    11881 gggcttgctc gttattcctg cacatataga tgagtttaat ggaatttctt cagcaaaatt
    11941 tggaaatcta ggaaagatat ttgaatcaga aaatatatat gcagtacaga cggttcaaaa
    12001 agaatttttt gaaaatcgct cacaaatgat tccctcatct aagcgtgaca ctatctatga
    12061 tagtgtgaat gccagatatg atggtagagt tggaaaagat actttggaat cttggtataa
    12121 atcagttgtt gaggcagaaa aaaataatat gacatttctt agcttttctg ataacccaca
    12181 ctcgaaagga aattcaaaac atggactttg gggaataggc acaagatatt cctatataaa
    12241 aatgaaggac gaacctgact tgggatcact tcgcgatgct ttaatgctag gcgaaactcg
    12301 agttcattct gatttttcta atttctccat taatgaaaat gaagttttat tggaaaaatt
    12361 aagcttttct gggaccactc ttagcacaaa agaagttgta gtcgaattca gcgataattt
    12421 aacttcaatt attggaggaa gaggaacagg caaatcttgt attacaagat tcttggtcta
    12481 tgtccttgga aaggaagcag aacttgatca attttctgaa atacaaagtg attatcagaa
    12541 ctttgcacaa attgaacata atggctctgg aatatttttg aaggatacaa ttgtgaaact
    12601 gaatattttc tataagggga caaaatatca aataataaga acacaggata gacattctat
    12661 ttatgaattt acacaagaga atggtctggt tgaaaaagaa acagaacggt tgagaatgat
    12721 ttccgataag gtgagtatat actctcaaaa acaaatttat gaaatttcaa aaaatcaaac
    12781 tagtatacta gaattactag atggatataa ctctgatttg atttcagagt ataaaaatga
    12841 gattgaaaca tgtgtaaacg aaataagaaa attaaattgg gatattgttt ctgtgaaaaa
    12901 agaaatacaa gataaggcga aggtagagct tgaaattgaa gatttaaaaa aacaagtaga
    12961 aaagctatct cataagagtt ataaagatgt ttatgatgag tattcaaaag agacagatat
    13021 ttatagggaa ctgaaacgtg atgtggaggc acttaaggaa attcccaaaa atctaactga
    13081 tagtgttgac aaaattgact ttggcaatgg tataaaagtt acagacgaaa ttgatgaaca
    13141 ccgcggagaa ttgattaaga atttagtgac caatcgggct aagattcaaa caataattaa
    13201 tgaaatgtca gatgaaattg atagttatag agcagagctg aataaatcag attggaaagt
    13261 taactataat gaggtttcaa agaggtatca aagaggtatc aaaaacttag tgaagagtta
    13321 tctcaagatg aattaaaaat gttacaaaac cttgattctt tatatcaatc tttatcaaaa
    13381 actgaagagc gattggaaga aattattcaa caagaaataa agcttaaaga ttaccaagtt
    13441 gaggttgact cagcaatgtc aagaatgaat aaatcatatc aaaatttcta ttctgagaga
    13501 agtagttcct cgcgcgaaat tttttctgat attacaggaa tagaggctac tattcaacca
    13561 aatagggatt tcaataatta tgttaaagag ctcagagctt gtattaataa ggaggacact
    13621 tttgacagtg agtttggatc tcttattgat aagctgacaa aaaattccat tactaatcaa
    13681 gatatatata atgacatact tgatataaga gttagtgggg aaagtagtat tttttcagat
    13741 aaaaaattaa tcaattctat aagaaaattg aatgatgcac aagttcttga attacaaacc
    13801 cttaagccaa atgatttagt agttatccga cttgaactaa atggaaaaaa agttagttta
    13861 tcgaatgcta gtgctggtca aaagacatct gcaattcttt ctatgatttt ggcgtatgga
    13921 aattcaccac tcatattgga tcagcctgaa gatgatttgg atagtcaatt aattaataac
    13981 ttaatcgtaa gaagtatcat tacaaaaaag gaaaacagac aaattttaat tgtaactcat
    14041 aatgcaaata tcccagttaa tggtgattca gaatggttga ttagcatggg agattcaaaa
    14101 gaaatatcgg ctgatcagtg cgggtcggta gatgaaccca atatcaagcc aagaatatgt
    14161 actgtgatgg aaggtggcga ggatgctttt acaaatagag cgaaacgcta tggttttaaa
    14221 caaattgtag aataacacta gagggtgaga catattatta tgtccgccct ttttaggagg
    14281 aaaaatgaaa aaaataaaaa gctataccag tatctgggca gttgaaaaag tcatttacgc
    14341 aatcaatgat tttcagttgc cttttcctgt gacttttaac caaatggctt ggttcgtttt
    14401 atctttactg tttgtcatcg tatttgccca tgtgcctcca ctatctatga tagaggaggt
    14461 ctttttaaaa tacttaggca taccggtggc tgtcacttgg tttatgtcgc aaaagacgtt
    14521 tgatggcaag aaaccactgg gatttttacg atcctttatc agctatcact tgcgatttaa
    14581 ggtgactttt gcaggaaaga aagtcaaaga acagaagaaa cgatgggatg aacccagtac
    14641 tcttgtaagg agtgtgaact atgtatccga ttaagtatat tgaaaataat ctcgtcttta
    14701 accaagaagg cgagtgtttt gcctactatg aattagtgcc ttacaattac tcctttttgt
    14761 cacccgaaca gaaatttcaa gtgcatgata actttcgcca actaattgcg caaaatcgtg
    14821 aaggcaagat ccatgcgtta caaattgcca cagaaagtag tattcgcgcc acacaagaac
    14881 gatcaaaaaa agaaattacc ggtagactca aagaagtagc gaaacaacga attgatatac
    14941 agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc ttttttattg
    15001 gctttaaact aattgcgaca gatgaagaag tcaatctaaa gagtctcaag aaatctttct
    15061 tttctgggct tcaagaattt gtctatggag tgaatcatca tttgatgggc gattttgttt
    15121 ccttatccaa tgaagaaatt cgctgctatt ctaagcttga aaaattaatg gaaagcaaat
    15181 tggctcgaag atttaaagtc aggcgagtca caccgagtga cctgacgtat ttgattgaac
    15241 atatttatgg cgaaaaaggg ataccgtttg aagaatacga atttcagcta ccaaagaaaa
    15301 agctaaaatc agaaacactg gtgaaacgtt atgacttatt acgtccaaat cgttgtttaa
    15361 ttgaagaaaa accaaggtgc ttacggatgg aacatgagaa tcatgagtcg tacgtagcgt
    15421 atttaacaat taatacaatt gtgggggaga tggaatttcc ttcctcagaa cttttctatt
    15481 accagcaaca acaatttaca ttcccaatcg acacaagtat gaatgtagag attgtgacga
    15541 ataaaaaggc tttagctacc gttcgtaata agaaaaaaga actgaaagat ttagacaatc
    15601 atgcttacca atctgacaat gaaacgaatt ccaatgtctt agatgctttg gattccgtgg
    15661 atgaattgga aaccaccttg gatcagtcaa aagaatctat gtacaaactg agttatgtgg
    15721 ttcgagtgag tgccgaatca gtggatgaac taaagcgacg ctgtgatgaa gtgctcgatt
    15781 tttatgatga taccaatgta aaactagtcc ggccctttgg cgatatgatg gggctacatg
    15841 aagaattctt acccttaagt aaacgatata tgaacgacta tattcagtat gttacgtctg
    15901 attttttggc ggggcttggt tttggggcta ctcagatgct aggagaatta gaagggattt
    15961 actttggcta caatgtggat acaggtcgta atgtttacct gaaaccggca ttggcttcac
    16021 aaggagtcaa gggttcagta accaatgcgc tggccgccgc tttcctgggt tctcttggtg
    16081 gaggtaaatc ctttagtaac aatctcttag tctactatgc tgttttgttc ggtggtcaag
    16141 ctgtgattgt cgatccaaaa gggggtgcga aacgttccta cttgaaaaga gtaggcacag
    16201 cacttcatta aatcaatatt taatgtgtgt tggagaacta aacacacgaa tagtcgaatg
    16261 acggggtaac gccctgaaag gctactttaa tactctgact ggcagaaact tgagtaaaca
    16321 aggctctgtc agaagctcgg tgaagtcggc agacagacac cgtaacagat aatgctgacg
    16381 aaagattccc agaggtgggc gttgtgtcct atatgccgga agtctataaa atatctatgg
    16441 ttagaatgta gatgagaggt ctactgacga atccttgaat gtacaggtct atatcggggc
    16501 tggatagaaa tgtccagtga cacaaacagt gtcgctactg tgggtgagta agactccgca
    16561 tttatgaaaa tccatataac gttaaaggcg ttatcaaggt agcaggctta tagaggaaac
    16621 ctaaggatat atgtacagat agtgtgtttg gaacgttgaa agctgacaac atggagattt
    16681 tactgtctgt gaagtgttca catcagaaag ttattgtgag attagcaatg atataaggtg
    16741 tgttaatgtc agtgagagtg gtggcacagt acctatgaaa ccgtgataac aagcggtgga
    16801 gggatagcca ctagtcatat gaagaataga attactaaga aaaatggtat gggttcgagt
    16861 aagactaaga gatgtacttg ctcctgcaaa caaggagggt acagaccatg ctaaagaaaa
    16921 acaagctccg ctataacgag tattatgata tgcaacatat ttatgacgag ttatacgcac
    16981 agagcaagaa cggtaataac ttttataagt tacttgaaat tattggttcg gaacaaaata
    17041 tttgtttagc ttaccgtaat ctgaaatcaa acagtggcag taaaacggct ggaacggatg
    17101 gaatgacgat agacgatata aaacaactca gcaatgctga gattgttgct accgttcggg
    17161 aaagccttag caactaccgc cccaaatcag tcagacgtgt tttcattcca aaagctggga
    17221 gcgataaaat gcgtccattg gggattccgt gtatctggga caggctggta caacaatgta
    17281 tcctgcaagt cctagagccg atatgcgaac caaagttcca taatcattcc tatggattcc
    17341 gtgcgaatcg aagtgcccac catgcagtca gcagagtaac gacactgata aatttatcaa
    17401 aatatcatta ctgtgttgac gtagacatta aaggattttt cgataatgtc aatcatggaa
    17461 aactattaaa acaaatctgg acacttggta ttcgggataa aagattgatt tgcattatca
    17521 gcaaaatgct gaaagccgaa attgacggcg agggtgtccc agaaaaagga acgccacaag
    17581 gcggtctgtt aagtccactg ctctccctca tagtccttaa tgaattagac tggtgggtaa
    17641 gcagtcagtg ggaaacattc cagccgaaga accgcagtaa aaatgggtgg cttcaatacg
    17701 caaagaaata taccaaactg aaaagcggtt ttattgtacg ttacgctgat gattttaaaa
    17761 tcatgtgttc aacctatgga gaagcacaaa gattttacca cagtaccgta gattttctta
    17821 ataaaaggct gaaattagaa atcagtccag agaaatcaaa ggtagtaaat ctgaagaaaa
    17881 attcatcaga ttttctgggg ttcaaaatca aagtcattcc aaaaggaaga acgaaacacg
    17941 gttatgttgc caaaacagat atgaaccaaa aagcattaaa gaaagcgaaa acaaatctaa
    18001 agttaaaggt aaaagatata gccagacata cgactggctt caatatcagc agatataatc
    18061 tgacagttat cggtatgcag aattactact gcatagcaac taacgtatat aacaacctga
    18121 cagaagtaag ttacgctctc ttaccgacca tacgaatccg tttaaggaac attgcaaaat
    18181 cagttccatt tgaaagtaca agttcagaat ttcagagcag gacaaaagga atcagaccaa
    18241 aaacaaaaat agtgatgatt gcggataatc cgctattgcc gattcaagga gtacaacaca
    18301 aaaatcctat gaatttctcg caagatattt gtaattttac aaagcaaggg agaaacaagg
    18361 tgcatgagga cgttgtagtt gtaacaaaag aagaaatccg agcgttgttg gaaaatgaaa
    18421 accctgccga tagcgtagag tttaacgaca atcgtatttc ggcatatatc gcacaacaag
    18481 gcaactgcta tgtaatgaat cgtcgaggaa ctccgtccac tctgatatgt attcacaaat
    18541 cagacaaagg agataacctt gacagatatt ctaatctggc atttgtggaa attcccattg
    18601 caaaggcaat cctaacagaa tcagcagacg aagcaaaatc actgttaaaa ggttatgttc
    18661 ttaatagtca gcagaagaaa aaactgaaca gaatacggac gaattacgga taccagacaa
    18721 taactgggaa ataacttata aatctatttg gatatgatgg aacgccgtat gaagcgaaag
    18781 tttcatgtac ggtgtgaagc gggggaaaac ttggagataa catcaaaaag ttacctatcg
    18841 ctatagcgag gagggtggaa agaaaccttg ccagaaattg cggatgaaat cagtatcctc
    18901 aacttgacga gtaagtcaga aaatcaaggt ctgcttgatc cttatgtgat tatgaagaaa
    18961 aagaaagact cggaaagttt agcaattgat attctgactt ttttaacagg aatttcaagt
    19021 cgagacgggg agaagtttcc tgtgttacgt aaagccattc gaacggtgac tcaaagtgat
    19081 caacgggggt tattacaagt tattaacgaa ttgcgaaaag acgggtcacc aatcgcggaa
    19141 aatatcgccg atcatatcga gtcctttacc gattatgatt ttgctcattt gcttttctca
    19201 gacggctcga ttacccaatc gattagttta gataaacagc taaatatcat tcaagtggcg
    19261 gatttggttt taccggatgc agaaacgact tttgaagaat ataccaccat ggaattgctt
    19321 agtgtttcga tgctaatcgt catttccact tttgccttgg actttatcca tagtaaccga
    19381 gaaatcttca aaattgtcga tttggatgag gcttggagtt ttcttcaagt ggctcaagga
    19441 aaaacgttat cgaataagct tgttcgtgct ggtcgggcca tgaatgctgg ggtctatttc
    19501 gtcactcaaa atagcgacga tttattagat gaacgtttaa aaaataatat cgggttgaag
    19561 tttgcttttc gcagtaccga tattcatgaa atcaagaaaa ccttggaatt ctttggtttg
    19621 gatcaggagg atcaaagcaa tcaaaaacgc ctgcgagatt tggaaaacg
//
LOCUS       DACDEA010000048        19450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDEA010000048 DACDEA010000000
VERSION     DACDEA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.048"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            209..994
                     /locus_tag="IV136_001806"
     CDS             209..994
                     /locus_tag="IV136_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8465792.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1703..2062
                     /locus_tag="IV136_001807"
                     /pseudo
     CDS             1703..2062
                     /locus_tag="IV136_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2357..3490)
                     /locus_tag="IV136_001808"
     CDS             complement(2357..3490)
                     /locus_tag="IV136_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8465793.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3481..4149)
                     /locus_tag="IV136_001809"
     CDS             complement(3481..4149)
                     /locus_tag="IV136_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465794.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(4605..5591)
                     /gene="lacD"
                     /locus_tag="IV136_001810"
     CDS             complement(4605..5591)
                     /gene="lacD"
                     /locus_tag="IV136_001810"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8465795.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(5604..6530)
                     /gene="lacC"
                     /locus_tag="IV136_001811"
     CDS             complement(5604..6530)
                     /gene="lacC"
                     /locus_tag="IV136_001811"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8465796.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(6543..7058)
                     /gene="lacB"
                     /locus_tag="IV136_001812"
     CDS             complement(6543..7058)
                     /gene="lacB"
                     /locus_tag="IV136_001812"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8465797.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(7077..7505)
                     /gene="lacA"
                     /locus_tag="IV136_001813"
     CDS             complement(7077..7505)
                     /gene="lacA"
                     /locus_tag="IV136_001813"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8465798.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(7525..8298)
                     /locus_tag="IV136_001814"
     CDS             complement(7525..8298)
                     /locus_tag="IV136_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8465799.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            8463..8942
                     /locus_tag="IV136_001815"
     CDS             8463..8942
                     /locus_tag="IV136_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8465800.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            8999..9301
                     /locus_tag="IV136_001816"
     CDS             8999..9301
                     /locus_tag="IV136_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8465801.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            9323..10780
                     /locus_tag="IV136_001817"
     CDS             9323..10780
                     /locus_tag="IV136_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8465802.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(11373..12125)
                     /locus_tag="IV136_001818"
     CDS             complement(11373..12125)
                     /locus_tag="IV136_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8465803.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(12340..12576)
                     /locus_tag="IV136_001819"
                     /pseudo
     CDS             complement(12340..12576)
                     /locus_tag="IV136_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(12586..13550)
                     /locus_tag="IV136_001820"
                     /pseudo
     CDS             complement(12586..13550)
                     /locus_tag="IV136_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(13756..15438)
                     /locus_tag="IV136_001821"
     CDS             complement(13756..15438)
                     /locus_tag="IV136_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8465804.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(15449..15772)
                     /locus_tag="IV136_001822"
     CDS             complement(15449..15772)
                     /locus_tag="IV136_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8465805.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(15795..17201)
                     /gene="lacG"
                     /locus_tag="IV136_001823"
     CDS             complement(15795..17201)
                     /gene="lacG"
                     /locus_tag="IV136_001823"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8465806.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            17668..18675
                     /locus_tag="IV136_001824"
     CDS             17668..18675
                     /locus_tag="IV136_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8465807.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(19069..>19450)
                     /locus_tag="IV136_001825"
     CDS             complement(19069..>19450)
                     /locus_tag="IV136_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8465808.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5943 a         4029 c         3201 g         6277 t
ORIGIN      
        1 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
       61 tttttgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
      121 ataccctctt attttagcat actccctttt agaaaaagca aaaaaaaaag aaaaaatctt
      181 attcttgata acaagctacg tgacgtatat gcctaacaaa aatagaactt ttgaatcaat
      241 cttaaatgaa ggtttaacca cagaaccggc atccattatt tttgatgaag ttatcgatga
      301 cgttgaagat agtaagtatc ataaaaattt aaaagaactt ttttattttg cactcaacaa
      361 tggaccaagt ttgttcactg gtatatcagt acctgatagt gataatccat taattatggc
      421 aaaaatttac ttaacaaaat ggtgtaataa atacatcagg gataggaata acccagcatt
      481 aaaaaaacct cttaaaacat ttggcgaaaa agatgctgct ttaactacta gagtagcagc
      541 aaatgctaat attgatgatc aaaaagtatt gaatgactac ttgcgtggac attttttata
      601 tatgagcgct gagaacatga acggaagtat tttagaggaa tatttagccg aagtgttaga
      661 acctgaaggt tggatttggt gcgccggctc tgtatatcga gctgttgatt tctgttattt
      721 aggaacctca ccaattcttt tacaagtgaa aaataaatac aatacagaaa gcagttcgag
      781 ttcagctata agagtaggaa caacaattag aaaatggaat cgtttaaata agtcgacaaa
      841 aatttctggt ttggatagcc ctatccccaa ttggaaagca ttaatagaaa tgactgaagc
      901 tagtaaggaa ctggcagcta agttaactga aaatagttat ttagcttata ttaatgaaaa
      961 atccacacga gaattatgga cactcgatga ctaacaatat ccttattagt atctatccac
     1021 gatacatgga aaaaattata aacaaacaaa aaaactttga atttagaaat ttctatctta
     1081 cctcagatcc ttataatatc ttttggattt acgaaacaat ccccaccaaa gctttaaagt
     1141 ataaattgat tgtaaaaaat ccaattacta aacttaagaa aaatcaacac tactttttag
     1201 gagacgatga gtttgaacaa ttagtttaaa agggaaaata tgcttctatc attacaagct
     1261 cgtttggtgt aagatgggta taagtcattt atgttcactc tccttgtatg ctttagcggg
     1321 tattacaatt tgagtgtaac ataaatggct tttttatttg tctcgcttaa ttatacaaac
     1381 ggcgaattat aaataactca taaaaaatat tttgactttc aaaatcactt ttgctaccag
     1441 aagttgaggc gaagtgtttt aagcatagtt gtaccctagt ttgaccgtta aaaataaata
     1501 acgatcgcta gagtgcaact atgtttgttt gccctaccgt cagcgtcggc ttggcctctc
     1561 acatccatta ccgtcgggat tatctccgtt acataccctt acaaaatacg ccctgtaagc
     1621 attgcatgaa accaccgtgc atttgcaccg tatttccgcc ctagccgttc aaatcggtcg
     1681 ctaccaccat tcagaaacac gtgcggtaat ggaaggtttg gaggaagcta tccagaccac
     1741 taacgattgt ccctatcgtc gtacgtttca ctcagatcaa ggttggtcgt atcaaatgaa
     1801 agcttataga aacaaattga aggagtacaa aatctttcaa agcatgtctc ggaaagggaa
     1861 ctgcttagat aattcactta tggagaattt ttttggctta ttaaaacaag agatttttca
     1921 cgggaaagtt tataactgtt tcgaggaatt aaaatcagcc atcgatagct atatctatta
     1981 ctacaataat gaacggataa aacagaaatt aaactggcaa agtcccgtcc aatttcgtaa
     2041 aacaaccgtt acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc
     2101 tccactccaa aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat
     2161 taattatatc cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc
     2221 ctaaagtagt agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac
     2281 aactcctttt tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt
     2341 atagacatct gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa
     2401 atacttccaa tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg
     2461 caacggattg aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat
     2521 cctgcttgag cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag
     2581 gaatgccaat tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg
     2641 tgacaattac cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg
     2701 cccgataaat ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa
     2761 acaaaagatt cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa
     2821 cgtctacgac ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa
     2881 tactgtcggt catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta
     2941 tttccagatt cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat
     3001 ttaatcgtcg ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg
     3061 catttaatgc ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac
     3121 gcgtaatcgc taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg
     3181 ttaataacat gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt
     3241 cgtcctggta agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg
     3301 aagcttccat ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt
     3361 cacctaaggt agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca
     3421 ctaaaatcac gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat
     3481 ttaatagcat agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc
     3541 ttcagccagt ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc
     3601 aaaatcttca tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg
     3661 ctgagcgatg cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg
     3721 atagctgacc cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag
     3781 cgcacgtcca tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc
     3841 aaagggtttg actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat
     3901 ctcatcacgt gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg
     3961 taaaacagat aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga
     4021 attgatgaag gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt
     4081 ctcctgctct aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag
     4141 tacacgcatg tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca
     4201 tagtacacgc tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac
     4261 attctgttaa aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga
     4321 aaaagactgg aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata
     4381 tacttttcaa agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc
     4441 atcaagtcat taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta
     4501 actcacttac tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg
     4561 tataaaaaaa gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt
     4621 gttaacactt ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt
     4681 ttacgccctt gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt
     4741 tctacaccat ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca
     4801 tttttcgcga agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa
     4861 ataaatggca ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt
     4921 gtgtacacga cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc
     4981 acctttaaca catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact
     5041 ttatgaggtt tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa
     5101 gaaacaagct ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca
     5161 ataaaggcat gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg
     5221 aacttacaag catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa
     5281 tctggtaaac ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag
     5341 ccggcatttt ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc
     5401 gatgaggcat atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat
     5461 tcttctgctt cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca
     5521 attgctaagg ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt
     5581 tcttcgttca tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt
     5641 tgataattgg ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa
     5701 acattaccct gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat
     5761 ccggcaacgg tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc
     5821 acgcaataaa aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact
     5881 atccattcaa tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct
     5941 gttgagattt ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta
     6001 ggctttacac tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca
     6061 ggctttggct gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc
     6121 aagcttcctg aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag
     6181 aacgcccttg cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt
     6241 ccgtcatgta aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga
     6301 gcaatctcat tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa
     6361 ccactagcaa tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta
     6421 ccgccagctg ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct
     6481 aatggataag aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc
     6541 gcttaatcgt ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga
     6601 tcagactgct tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt
     6661 tcttcagttt cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat
     6721 tctcccgtaa tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag
     6781 atagcagtcg tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg
     6841 ttgttaatcc caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc
     6901 acggcctctc cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc
     6961 cccatatcca acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta
     7021 tctgtgacga tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac
     7081 gccattttat ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt
     7141 agaaattcct taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg
     7201 atcatccgag aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct
     7261 gctgctacca ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca
     7321 accgcgattc ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat
     7381 tccacaaaat cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc
     7441 aattttttct tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca
     7501 cgcatttttg tttcctccta ttttttactg aatgatttta gtgaattgct gataataact
     7561 cttcagctcg gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata
     7621 gaaattataa aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc
     7681 cgcattatct aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc
     7741 cgaacccttc aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc
     7801 ttcattggca attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa
     7861 atacagttca aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac
     7921 gattcggaga gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc
     7981 aagaaaaatc gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc
     8041 cttctctttt gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga
     8101 agtcgacatt tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt
     8161 cttctgcgcg agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc
     8221 ctgaataaat ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg
     8281 tcgttctgtt ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca
     8341 tttttcaaca catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg
     8401 gttatactca acttgtaaac atataaataa acataaacga actaataaag gagctgtttt
     8461 gtgtgaatga attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag
     8521 aagtgttcga ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt
     8581 tggagaacct tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct
     8641 ctaaaatatt gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc
     8701 gtatcattcc aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg
     8761 atcagatttc cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg
     8821 gcatgttggc taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt
     8881 ttttcaaatt agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat
     8941 agaagtctct gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat
     9001 gatcaaaatt ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc
     9061 tttggaaact gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa
     9121 agatatcaat gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga
     9181 cttgggtttt gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc
     9241 tgctatggct gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata
     9301 ggactaaaga aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac
     9361 ccccctaatt aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt
     9421 attttttaaa gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct
     9481 gactgggatc ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc
     9541 ttttgttgaa cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc
     9601 aaccatcaca tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa
     9661 tattgtcatg ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg
     9721 gcatttagca attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc
     9781 tgcattagtt attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac
     9841 cttcaatgac ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta
     9901 catgatgaat ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt
     9961 ggataaatat gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa
    10021 attcgcgatt ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac
    10081 gcaaattttc tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg
    10141 cgcttggttc atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa
    10201 acgactaaaa ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc
    10261 tgaaatctgg gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt
    10321 accaggaaat aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact
    10381 attggtcgta actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc
    10441 attattctta tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt
    10501 cggagccttt ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg
    10561 cccgattgaa aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt
    10621 tgtgatatac gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg
    10681 tcaaatgaaa aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc
    10741 aacggcgcat aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt
    10801 ctttagactt cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca
    10861 aaaagcatag cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt
    10921 ttaactcctt taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt
    10981 tgttttgtgc tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct
    11041 gaatcgcttc gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac
    11101 tcgtttcatg tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt
    11161 tcctccttaa taaagcggtg attgatactg ctattttttc cttcacataa acataacatc
    11221 ccaaaaaaat cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa
    11281 tgatgcaccc taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag
    11341 ttctaattat ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg
    11401 attttttcat agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga
    11461 ttataatata ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt
    11521 tcaacagaat tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata
    11581 attttctgtt catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt
    11641 agcgatttat tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct
    11701 aatagataga tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag
    11761 tttgtaacaa tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt
    11821 cctggtccaa gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc
    11881 cgctgctttt cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga
    11941 taaaaatcct tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt
    12001 tcgtccaaat cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg
    12061 actcgtactg taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt
    12121 aacatttcat cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac
    12181 aagttttctt aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct
    12241 gctattctaa aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc
    12301 gacttttatc tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac
    12361 aaagtagtta atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag
    12421 cgaaaaaccc tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc
    12481 gctgatcgta tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata
    12541 acccatttct tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga
    12601 caatatgtct ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga
    12661 ttttcttgga atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc
    12721 cctaaagtcc attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcgtg
    12781 ctcatttagc cctctttgcg aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt
    12841 cgagttactt atagatcgcc agttggaaaa ttcttttcca caatcaaatg tcatggattt
    12901 aaacaagtgt ttaggcatgg atttgaacca atcatttaac ctgttttcga tatcttcggc
    12961 tcgtctgcct gtcggtttca aagtaataat aaccttggat aaacactcaa ccaatgtaat
    13021 aacagcgctc ttgtgtttaa ctcccacaat ggtgtcgcct tcgagatgac caaattcttt
    13081 agtcaggtgt ggatagagac tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc
    13141 acgcttttct tgatgattat tcggtttacg tttccccttc ataggtaacg actgtgtgtc
    13201 aaaaaggcct tttttaaaca tccgatagag tgtacgaaca gaacaatcaa ttggaaactc
    13261 gccacggccg ataatcacat cgggtgacca cccttgagct accttttctt gaacatatgt
    13321 tgattgttca tcagataaaa cggttgaacg tcgtccacaa cgagatttat tttgtttgta
    13381 ttgacggtaa taatctagca cttttttcct tgtttgagaa acgaataaac attataaatg
    13441 gtttgtctag agcgatttaa actttgtgcg acaagagcga caggtttctc gatattgaaa
    13501 taagactcta tgattactag ttcgtctgtt gtaagatggg tgtaggtcat ttgtactcac
    13561 tccttattaa ttctttggtc ggaactattt tgagtgtagc acaaatgatc tttttagttg
    13621 tctagcttaa ttttacaatc gacgcctcaa aaagaatacc cgaaatttaa acagcaaaaa
    13681 aagatagcgc ttaccattgt gtttaagata aaacgctagc taagttggct agcgctttat
    13741 taatctaagc aagctctaaa attgcttcaa tacaaaatca actgctcctt taggatcacg
    13801 tgttaaattg atatattctg cacctttagt tgcaattact tttatcccta atttgtctgc
    13861 atctttacgg agatcatcca aataggaatt cacttgaggt gctagaacga ttaaatcaaa
    13921 atttttcatc atatcataat gagaaccata agctcctgct ttagcagaaa gattaacacc
    13981 tgtctctttt gcgccctctt ctaatgcatt agccaacatt gcactcgttc cggcaccagc
    14041 acaaagtact aaaacgcgct ttccttcacc aaccgatgct tttgcttcat caactataac
    14101 tgccgcagtt gcaccaactt cttggatttc ttcgaccaat tcttctttta cttcttctgc
    14161 agcaatgttt tttaactctt gttctactaa ttcagaatca taggccttaa caaaaggtaa
    14221 ataaataatc gcatcgacaa caatcaatac taccgctaat acaatcgcca acacactaaa
    14281 cccggttcct aaaaccaagc caatcggtgc gggcgtcgcc caaggcaata cggatataaa
    14341 gctattcatc cccaagacat cgacaaaaag tttgaatatc catacgttag cgattggtgc
    14401 taataaaaat ggaattagaa agtacggatt caaaataata ggagtagcaa atagtaacgg
    14461 ttcatttacc gcaaacgaaa ctggaataaa tgaagcttta ccaactgctt tcaattgttt
    14521 tgatttagcg aacaacatga acagaaaagg aacaactagt gtcgctccag taccacccat
    14581 cgttccaaca aagtttccta gaccaactgt taatactttt gaggcttgtg caccgttttt
    14641 aaataattca aagttagttt ctacgttcgc ataaataatt gcagcaatcg ctggttcaac
    14701 aatcgatggt ccatgaacac caacaaacca gaacattgcc atggctcccc aaatgattgc
    14761 aataccgaca tatccatcag cagcactaaa taatggctgc aataattgaa ttacacctgc
    14821 tgcaaatgat gttccaaaaa attgacgaac tcccgtatca atcaaaacgg caataaatac
    14881 tgcaattgaa aaggggataa tatctctaaa catctgtgtg attgttccgg gaacttcttt
    14941 tggcatacgg atcgtcaaat ctcttttaac acagaattta tacacattta cagtaacaaa
    15001 cgcagaaacg aaagctgcta ataatccctt tgtccctaaa taatcagtag ctatcccacc
    15061 atcaatttga tcagctgcca tcattacaaa accacaaatt gacgctaaca taacagaaac
    15121 ttcattaatc tgcttacctg aatcagataa ttgacggttc attgaattag ctaaacatcg
    15181 agctgttgta gcaacaacca aaagaccaac taatcccatt gaataaccat aaactttcca
    15241 aagccacgtc gagaatgcct ctggtaacgt cacgttaaat acttctggaa cagccgcaat
    15301 cataataaaa atacttgaaa atagaatagc tggcatagca gttaaaaagc catcgcgaat
    15361 tgcccctaaa taaatattac gtgagatttt ttcaaaaaac ggttttccct tctcaatttg
    15421 cttaataatc gtattcattt ttcttctcct atgctttagc aaatttttct ttgtacaatt
    15481 caacaaaatc attgataagt tctcttaata gaatcgttgt cattaaatgg tcttgcccat
    15541 gaataaaaat tacgcctact tccatttcgt tccctgacgc ttcttcagct aaacatttcg
    15601 tttgtgagtt atgcgctaag gttaaatttt catcagcatc cgctaataat cgttcaacat
    15661 tatcaaaatt accttctctc acttccttca acactgttaa taatgaactt ctggcatctc
    15721 ctgcataagc aacaatttca aatcctaata attgcaattc tttcctatcc atttccttgc
    15781 cctcctaact atttttatat tatcgttttt gtttcgctaa caaatttata ccaatatgca
    15841 gactctttcg gatatctttt ttgtgtatcg aaatctacat agaatagtcc ataacgctta
    15901 gtgtatccat taacccaaga gaaaacatcc attaatgacc aaatgaaata cccctttaca
    15961 ttaactccca ttttgatagc atcggaaatc gcattaaaat gctggcgtaa ataatcgatt
    16021 cgatcatcat ctagaataat cccatcttca aaattatcct tataccctag cccgttttca
    16081 gtaatgtata ttttattata atttggatat tcgtcttgga ttcgtacaag taaatcatat
    16141 aacccttttg gataaatgat ccaatcccaa tcggttttcg gaagatcctc acgcacgatt
    16201 ctctcgccaa tccctttaat gcaatagact tggctacctt tttctccggt agtgttgtag
    16261 tgaagtgtgc tttctccttt gtactcttta acccaatggc taccatagtt attgatacca
    16321 agaaaatcat ttcgatttgc tgcttttttc atctctctaa aatcattaga atcaatatct
    16381 gcagattgcc catttgctgc taaaatctca ttaatatgtg ccattgtttc atctgagtaa
    16441 taccctaaat aagtagcatc taataagaac ttaattgata acgcatcatc taatttcgca
    16501 gcatgcttat cttcttcttt gtcacttgct ggatattttg tctctagcga gtgaacaaca
    16561 ccaatttccc ctttatatcc accatcttta aaaatgttta ctgtctttgc gtgcgcatac
    16621 atcatgttgt gtaaacaagt tacagtcttc tctatatcaa attgaacttc tggaggaaac
    16681 gatccgatca aatattgatt tgaagcaact ggataaatct cattaaatgt actccaataa
    16741 tgaacctcag aaaattcatc gaaacagaac tgtgcatagt ttacgaaatg ttcaacattt
    16801 tctcgattta aaaaatctcc attatcaaat agttctttag gggtatcaaa atgatgaaga
    16861 gttacaaaag gaataatacc atgcttattc aattcagcaa ataccttatg ataatattcc
    16921 actcctgttt ggttcacttc cccatagcct tttggaaaaa tacggctcca tgcaatagac
    16981 atcctaatac tatcaatttt aaattttttg cataattcag catcaactgg atattgatta
    17041 taaaaatcac tagctggatc agcagtatag cgtccctttt ctacaaggaa agtatcccac
    17101 gctacttgcc cttttccacc ttcttttgta gagccttctg cttgataagc tgccgtagct
    17161 cctcccataa taaagtcttc tggtaaagtt ttataattca ttttaatctc tccttgaaaa
    17221 ttagtttgat caatatgcca ataatccata aatcacctca gcataatttt aacatgtata
    17281 agtaaatttt tttacacaac gaaaaaccga cctcgaatat ctattttatt gcgatataca
    17341 agcacttata tcccttgatg taaaataaat ataccattca aaaaccgttt tgtaaacgtt
    17401 tttttgtaaa ataaatttat ctttttacta atttaattta cacgaaacaa gaaaacggac
    17461 tgattcccac taaaatcaat cccacctact aatcattctc caagtggcta tccattatcg
    17521 attcaatctt ctctgttaat ggattaggct ctctgctgat atcagagttt cgtttcctgc
    17581 tagaaaaagt aatttaagtt actaaaagag cagtttcgat ttactttaac aaagaaaagg
    17641 attattatat ccctaggagg atgaacaatg gttactatta gagatattgc aaaaaaatct
    17701 ggttactcaa cggctactat atcaagactt tttaaaggtg atgagtcact ctctattaca
    17761 tctgatacta aaaacaaaat aatcgttaca gccaaatctt taggatatga tctgacaaaa
    17821 attaagacta ctctgtacaa gattgccatt cttttttgga tctcagagga acagttaaaa
    17881 aaggatgtct actatcaaca tgtcaaagat gatttgaaaa attatagtaa aaaagctaat
    17941 ctagatttga ttttcatctc taaaaacgat ggtgtaaaaa gcattcctga cgatattact
    18001 ggatttatcg caataggaca attgacaaaa aaggaattgt actacttaaa tcaaaaaggg
    18061 tataaggggg taatccgagg aataaaccca ttacctgaac acttcgatac tgtagaacct
    18121 gatttccacg aaatggccag acaagcaatt aactacttta ttaatgaagg tttcacaaaa
    18181 attggattta ttggaggaaa tttctttaac ctagaaacgg gtaaagaaga gattgatagc
    18241 cgtgaatatg ccttccgata tcatttagaa aagagcggac tactcaatga aaagtatatt
    18301 ttctcccaag gatcatttac tgttgacgaa gggtatcaat taactcttaa aatgaaagct
    18361 gagttaaatg aagatatgcc cgaggcatgt ttcataccat ctgacactat agctgccggc
    18421 gttcttcagg gctgccatga attgggtatt aacatcccta aagatatggc agtaataagt
    18481 attaatgatg acgacatcgc aaaattcgtt gcaccaccac ttacaacttt taagatagat
    18541 atcgaagaga tagctaaaac ctgtgtagat ttattaagtg atcaactggt atattcgcgg
    18601 gacatatcaa agaaaacatt aatcggcgca aaattgaccg ttaggaaaag ttttatccct
    18661 aaagaagagg tgtaaaataa gtaattgaaa cttatcttta aaactataat tgtgtccaca
    18721 tagtagccga gcaatcgggt ttatgcctat tatgttttcc ataaataatt tgatggtgat
    18781 attttgacgt aaatacaata tgattttttc atcgccaaat tgtgcgcgct aatttttgtt
    18841 tatcattcag aacatttcct cctttttatc taagtatgag gttggataaa cgcttcatct
    18901 tagtatcttg ggggattttt tatctcgttg ggaatatatc aagcatcaga gcgcgcaaaa
    18961 agaactggtc gaaaaatcaa tctaccagtt caatctcatt cactgtgata ctatgtttta
    19021 tacagataac gccatattat aaaacaagtt gtattccgca aatgggttct attcaactct
    19081 taaactatct ataaaagatt cagccaactc cattagcctc tcgtaattta gttggtgaac
    19141 agcacttttt tcaaaaaatt ttgaaccaat tcctacataa gaggctccat tgttcaaata
    19201 gttttgtgca ttagtctgat ctacccctcc tacagccatc aaaggtaaac ttcctaaagg
    19261 ggcttgcact tgattgaaat attccggtcc aagacagttg gcggggaaca ccttaacaat
    19321 atcagcaccc atctcaaaca ttttgaatat ctctgtgggc gacatggccc caggaattgt
    19381 tagaaggttc tgttgcgaag tttgaaaatc aaacgcgccc tctctgaact ccaaatatct
    19441 taggctgtta
//
LOCUS       DACDEA010000049        18443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDEA010000049 DACDEA010000000
VERSION     DACDEA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.049"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IV136_001826"
     CDS             172..516
                     /locus_tag="IV136_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8465809.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IV136_001827"
                     /pseudo
     CDS             517..936
                     /locus_tag="IV136_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IV136_001828"
     CDS             complement(1028..1378)
                     /locus_tag="IV136_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465810.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IV136_001829"
     CDS             complement(1344..2675)
                     /locus_tag="IV136_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8465811.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IV136_001830"
     CDS             2931..3497
                     /locus_tag="IV136_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8465812.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IV136_001831"
     tRNA            3642..3729
                     /locus_tag="IV136_001831"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IV136_001832"
     CDS             4063..5037
                     /locus_tag="IV136_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8465813.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IV136_001833"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IV136_001833"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8465814.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="IV136_001834"
     CDS             complement(7452..7898)
                     /locus_tag="IV136_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8465815.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="IV136_001835"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="IV136_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8465816.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="IV136_001836"
     CDS             8363..8881
                     /locus_tag="IV136_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8465817.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="IV136_001837"
     CDS             complement(8958..9914)
                     /locus_tag="IV136_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465818.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="IV136_001838"
     CDS             10080..10886
                     /locus_tag="IV136_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465819.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="IV136_001839"
     CDS             10883..11872
                     /locus_tag="IV136_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8465820.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="IV136_001840"
     CDS             11869..12873
                     /locus_tag="IV136_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8465821.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="IV136_001841"
     CDS             13005..13547
                     /locus_tag="IV136_001841"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8465822.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="IV136_001842"
     CDS             13567..14265
                     /locus_tag="IV136_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8465823.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="IV136_001843"
     CDS             14286..14498
                     /locus_tag="IV136_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8465824.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="IV136_001844"
     CDS             14498..15460
                     /locus_tag="IV136_001844"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8465825.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="IV136_001845"
     CDS             15478..15873
                     /locus_tag="IV136_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8465826.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="IV136_001846"
     CDS             16036..16401
                     /locus_tag="IV136_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8465827.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="IV136_001847"
     CDS             16398..18209
                     /locus_tag="IV136_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8465828.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="IV136_001848"
     CDS             complement(18271..18438)
                     /locus_tag="IV136_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8465829.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         5927 a         3122 c         3810 g         5584 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaattg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaaag
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aat
//
LOCUS       DACDEA010000050        18341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDEA010000050 DACDEA010000000
VERSION     DACDEA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.050"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(110..565)
                     /locus_tag="IV136_001849"
     CDS             complement(110..565)
                     /locus_tag="IV136_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465830.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="IV136_001850"
     CDS             complement(719..1927)
                     /locus_tag="IV136_001850"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8465831.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="IV136_001851"
     CDS             complement(2039..2371)
                     /locus_tag="IV136_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8465832.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="IV136_001852"
     CDS             2519..3394
                     /locus_tag="IV136_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8465833.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="IV136_001853"
     CDS             complement(3476..4057)
                     /locus_tag="IV136_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8465834.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="IV136_001854"
     CDS             complement(4063..5322)
                     /locus_tag="IV136_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8465835.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="IV136_001855"
     CDS             complement(5324..6370)
                     /locus_tag="IV136_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465836.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="IV136_001856"
     CDS             complement(6605..6880)
                     /locus_tag="IV136_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8465837.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /locus_tag="IV136_001857"
     CDS             complement(7123..8433)
                     /locus_tag="IV136_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8465838.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IV136_001858"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IV136_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8465839.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="IV136_001859"
     CDS             complement(9979..10659)
                     /locus_tag="IV136_001859"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8465840.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="IV136_001860"
     CDS             complement(10708..11343)
                     /locus_tag="IV136_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8465841.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="IV136_001861"
     CDS             complement(11409..12812)
                     /locus_tag="IV136_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8465842.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="IV136_001862"
     CDS             complement(12799..13830)
                     /locus_tag="IV136_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465843.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="IV136_001863"
     CDS             13861..14082
                     /locus_tag="IV136_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8465844.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="IV136_001864"
     CDS             complement(14247..14984)
                     /locus_tag="IV136_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8465845.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="IV136_001865"
     CDS             complement(15137..16477)
                     /locus_tag="IV136_001865"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8465846.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="IV136_001866"
     CDS             complement(16714..18237)
                     /locus_tag="IV136_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8465847.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5177 a         3907 c         3103 g         6154 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca attaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa c
//
LOCUS       DACDEA010000051        17734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDEA010000051 DACDEA010000000
VERSION     DACDEA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.051"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            81..821
                     /locus_tag="IV136_001867"
     CDS             81..821
                     /locus_tag="IV136_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8465848.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1245..1415)
                     /locus_tag="IV136_001868"
     CDS             complement(1245..1415)
                     /locus_tag="IV136_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8465849.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1609..1788)
                     /locus_tag="IV136_001869"
     CDS             complement(1609..1788)
                     /locus_tag="IV136_001869"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465850.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2175..3287
                     /locus_tag="IV136_001870"
     CDS             2175..3287
                     /locus_tag="IV136_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8465851.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3365..3658)
                     /locus_tag="IV136_001871"
     CDS             complement(3365..3658)
                     /locus_tag="IV136_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8465852.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3826..4140
                     /locus_tag="IV136_001872"
     CDS             3826..4140
                     /locus_tag="IV136_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8465853.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4161..6140
                     /locus_tag="IV136_001873"
     CDS             4161..6140
                     /locus_tag="IV136_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8465854.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6153..6479
                     /locus_tag="IV136_001874"
     CDS             6153..6479
                     /locus_tag="IV136_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8465855.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6495..7013
                     /locus_tag="IV136_001875"
     CDS             6495..7013
                     /locus_tag="IV136_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465856.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7013..8365
                     /gene="celB"
                     /locus_tag="IV136_001876"
     CDS             7013..8365
                     /gene="celB"
                     /locus_tag="IV136_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8465857.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8449..9954)
                     /locus_tag="IV136_001877"
     CDS             complement(8449..9954)
                     /locus_tag="IV136_001877"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8465858.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10046..10636)
                     /locus_tag="IV136_001878"
     CDS             complement(10046..10636)
                     /locus_tag="IV136_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8465859.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10815..12461
                     /locus_tag="IV136_001879"
     CDS             10815..12461
                     /locus_tag="IV136_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8465860.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12510..13229
                     /locus_tag="IV136_001880"
     CDS             12510..13229
                     /locus_tag="IV136_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8465861.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13708..14655
                     /locus_tag="IV136_001881"
     CDS             13708..14655
                     /locus_tag="IV136_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465862.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14667..15587
                     /locus_tag="IV136_001882"
     CDS             14667..15587
                     /locus_tag="IV136_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465863.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15628..17085
                     /locus_tag="IV136_001883"
     CDS             15628..17085
                     /locus_tag="IV136_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8465864.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17430..>17734
                     /locus_tag="IV136_001884"
     CDS             17430..>17734
                     /locus_tag="IV136_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8465865.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLI"
BASE COUNT         5570 a         3034 c         3610 g         5520 t
ORIGIN      
        1 ctcaagcgcc aacggggtat gaattagatg cgacaccgat cgagtttgag atcgaaagaa
       61 gtcagacagc ggtggtagaa ttgacgaaag caaaccgact tacaccaggc ggtgtcgtgt
      121 tgacaaagat cgatgaccaa agcggggaag tcctgcaagg agctgttttt gagttacaag
      181 atgccaatgg aaccgttctg cagtcaggtc taaccacagg agcagatgga aaactggcga
      241 ttgacggctt agcaccagga gtgtatcagc tggtagaaac ccaagcgcca acggggtatg
      301 aattggatgc gacaccaatc gagtttgaga tcgaaagaag tcagacagcg gtggtagaat
      361 tgacgaaaga aaatcgactt acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa
      421 gcggagaagt cctgcaagga gctgtttttg agttacaaga tgccaatgga accgttctgc
      481 agtcaggtct aaccacagga gcagatggaa aactggcgat agatggctta gcaccaggag
      541 catatcagct ggtagaaact caagcgccaa cggggtatga attggatgcg acaccagtaa
      601 ctttcaagat cgaaaaagaa caagaagtgg cgatcttttt aacaaaagag aatagaaaag
      661 ttgctgatag ctcagatata agaggaaaca atccaacaag cagtggaaat aagcatcttc
      721 caaaaactgg agaaaccatc attagccagt ttatattgtc aatattgggt gtgctacttg
      781 ttttcatcag tataaaattt agaaagaaac gaaatatatg agctaaaata aaaaattgaa
      841 taagcatagt ttttaaaatc aactagtacc catcaattga atggaaacta ccgattttag
      901 aaggggtata caaacaaata ttagtatcgg agtagagaag gttgaaccca accagtaaat
      961 aagtaaagac aattttgtgc tgcgttgaga agtgaaaaat aaacctttgt agtaatacta
     1021 gttaataaaa aatatacaaa aacactgcga aaatcattct tgagtttcgc agtgtttttt
     1081 gcctaatgac tcaagtttat tgggtaatac tttgattttc tttctacaaa taatcagcga
     1141 tcagttgtaa ttttttctct agttttaatg gctctttgtc aataaggaga aaaacaagtc
     1201 gggtcttttg tctcggaaag cttcaaccaa ctcgtgaata aaggttacgc ctccttgaaa
     1261 ggcgtggaaa aaccaagtaa tcgatcaatc accaatttcg catttggtag aacatgtttg
     1321 gtgagctgaa agtaggtggc gttcatgtct gtaactaaga attctatttc tttaggatta
     1381 gtgcagctaa ggaaatagtt tgttaatcga ggaatttttt gtgtaggtaa attttctcga
     1441 ttaatttttc tgtctcgcca tccgcggaaa taaaactcag tttatcctca acggaaacgt
     1501 gagaacgaaa ttcacaaccc tcaacactca agacagaatt cttttggaag actttggtaa
     1561 atagcttctc aactctttca atgtgcgaat aacggttgtc aaagatacct aacaactttt
     1621 cgaaataaaa aataacgaca ctttttcagt cagtaaagaa gtgattttat gtctgacatg
     1681 atttgaaatc gagtgccttg gacggataaa ataactttga gcgttccaat ggcttcgaca
     1741 gtttttacag gtatagcgct gcttttttag gcgtataacc attggcatat gattatatta
     1801 ttcaaagtgg acaatggttt ccttcttctt gtttttcacc accattaatt tcctatttcc
     1861 atctaccaca gcagaaccac aatttctaca gacacatgga gcagggaaga gaactgcagc
     1921 aacgatcaat gtttttttct tctgtaaggt ttcgtaagag acctctgtaa tcatcaaatt
     1981 tttgtctatt gatctcagaa tttttttgat agaaccattc ataaagcata tcgttctctc
     2041 agttgtttat tttgtggtga tttaatcata caagaaaacg atatgttttt ttataaaaac
     2101 atgaatagga aaaatagttg acaagaaaat gacaacgcca tatgatgttc catagtattt
     2161 acaacctgtc atagatgact gtaaaaaata aaagtaaaaa gaaaggacga caacaggtgg
     2221 atttttatca gtctctacaa ctagatccgt ttattttaaa acaaaaaata cgagaagcag
     2281 cttctaaaaa agaaaagtgc atgtatattt gttcattgtt tttacgatca ttattcatcg
     2341 tattgtttgc catttgtttt atcattataa ttacaacatt atttgaatcg aagcataaac
     2401 catacgctgt ggttttattc tgtatgttga tgagtattcg ttttgttgac tttggttata
     2461 agatttccca ttcgatcata ggtttagcaa tcgtcatgtt ctcactatta gtagcaccgt
     2521 atgtccagct gatcaaatgg tcagttatgg gcatgttgat tcattttatt ttactaagca
     2581 gcatattaat ggcaaccgct agtgatccca aaatggggaa tgcttctttg tacggctttt
     2641 catatctctt tattgtttat tcgttaccta aagattcgtt gaacaagaat ttctttacac
     2701 aaacaagtag cttattattt ttgttttttt gttggttttc agttctcctt tacagaaaac
     2761 atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat ttttttgaaa ggcatgtatt
     2821 cgcaggaaaa aatttggatg ctcatctatg catttggcat tagtcttttg atcgttgcag
     2881 gagagtatgt cccttttcag cgattgatgt gggcagggtt tgcattttct tctattgtat
     2941 ctagctatgg attgatgtcg attggtttca aagaacgagc agtagaccga atcattggct
     3001 cattgattgg atgtgtttta tttattggta tcagccaatt tattcctttt aactgggtcg
     3061 gtatattagg tggtctagct ttaggtgttt gttctaccta tagatataaa acagttttta
     3121 attcttttgg tgctttggca atcactgcca gtttattcgg ggttccagga gcagtaacga
     3181 tccgcatttt tgagaatatc cttggggtat gtttaggaat catgtatatt ggggtgactg
     3241 aaatacttat ccgaaaaata agagaaaaac acggtttgaa tcattagatg agatcgaata
     3301 aaaaataagg aaaaagtgaa gatacacatg gatgataaat tgctatcatt ccatgtgtat
     3361 ctttttacgg taaatcagtg aaacgttcga ctttcatttt taatttatac tttttacgga
     3421 gttcgtttat ttgagccatc atttcggatt tgtgatgctt ttcgattgct tcttcatctt
     3481 tccaacggtc gatcagtaaa agagattctg gatcttctag tggaaagtaa tagtcatatt
     3541 tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt tctttcgacc atttcttctg
     3601 caaattttct ggcttgtcca tttgtacttg tatacaaaat attcactgtg atactcacta
     3661 aatcccctcc tgaaaaaatt ctatcactag tatatcacgg tgccagaaat tattattcaa
     3721 gatatgatac acattttttc cgcaatgcta cggaaatata tattttgaat agaaaacgct
     3781 tacaagatat gcttggttta taaaaataaa aggagtgatt gatttatgaa aaaagcattg
     3841 attatttgtg cggcaggaat gtcatcctcg atgatggcct caaagacaac ggatttcttt
     3901 aagcaaaaaa gagaagaggt actcgtagat gcagtttcag ctacagaagg cgataatatg
     3961 attaagaata gtgattttga tttatttctg atcagcccgc aaacgacgat gtttttggat
     4021 aaattcaaaa aattaggaga atctgttgga aagcctgttg ttagtatccc tttccaagcg
     4081 tatgtcccaa tccctacagg catccagaag ttagcagagg tgatcgaaga gaacatatga
     4141 tggaatagga gtggatgata atgaaacaga aggaaaaatt gctgttgaga tatttgattg
     4201 atcacaagaa ggagtatgtc accagtcaac gtttagcttc tgaattgtta ttatctgatc
     4261 ggacaatccg taattacttg cagcggatca aagagttagt agaaaaaaac ggaggtaaga
     4321 tcattgctaa accaggttat ggctaccaac tgcatatttt acaaagactg acatttgact
     4381 tgtttttgag tcggcaagaa atagtacctg gatatgctag agaaccgcaa ggattttatg
     4441 agtccgaaga tcgacaaaag tatatattga accaattgct tttggaagat gccattcttt
     4501 ttatggatga tttggcagaa gagctttata tcagtcgttc cagtttgaca aaggacatgc
     4561 aagaaatcaa ggagcgattg attccatatt cactaaaaat cgtttctaag catggtcaag
     4621 ggacatggat cgaaggggaa gaaagaaatc gacggcattt tatcatggat actttttttg
     4681 gaaaccatta tggaaattca ttgaaagaat atttagggca aagccgtttt ttcccagaca
     4741 tcagttttga agagctagtc attatcattc tcgatgaaac aagagaagct aagttaaaag
     4801 tttcggattt tatcatacag aatctgactt tacacttggc tttggggatc aaacggctcc
     4861 gagaagggtt tgaaatcaag gaattaggca tcgaacaaga agtttctaag cgagtagagt
     4921 atcaagtggc gcagcggatc gttcaacgta ttgaggtaat cgcaggtgta tcgtttcccg
     4981 aagaagaaat tgcttatttg acattacatc tcatggctaa gtcgaatcac aaagaaaatc
     5041 aaaatgatca ggaactatca aaggaattag cgaaagttct gaaaagattg tctcaggaat
     5101 ttcatttttc attagtagag gatctccagc ttttcaatgg actactcgat catttgtcac
     5161 cgatgatgat tcgattgagc cgaggtattc gcattgagaa cccattgact gaggagataa
     5221 agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca tttcagcaac atgccggctt
     5281 tgaaaaacta taccatcaat gaagatgaat gggcctattt ggctttgcat ctcatggcgg
     5341 cattggaaaa agaaagagct gcgcataaac tacatgcatt gattatctgt gcaacgggtt
     5401 acggcagtgc ccaactatta aagaatcgcg tagtcagtga attcggtaaa aatattacag
     5461 tggtaagtgt aaaaggctat tatgagatca atgaaacaac tttaaaagga atcgacctga
     5521 ttatttcttc tattgatctt tcgacaatgt ttttcaaaat tccagtttta catgtcagta
     5581 tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt agttggagaa tctataccct
     5641 atcattcctc agatagaccg ataccagcat tttctctaca gcaaaaaaag cagatttaca
     5701 ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta cgaaccgaaa aaggaagaaa
     5761 ttcttcatga tttgctgcaa aagctgagca taaatgaaga agaaggatat ataagagaaa
     5821 tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat catattcagt gatacagtgg
     5881 cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa ggtagccgtt gcattgattc
     5941 cttctgggat gcagtgggat caatatcaag aaattcattt tgttttttta gtttcgcctt
     6001 cttatgtcga aaatgaagga attacggtcg tgactaaagc aatcgttagg ttagttgacc
     6061 agctaaaagt acagcaggaa atcttagctg aacctatttt tgagaatttc agcagtaaat
     6121 ttatcaaact gatcgaataa ggaggaaaac agatggctga aaaaataaac agtgaaacac
     6181 tacaagtgac ttcttttgat atcattttcc atagcggaaa tgcacggaca ctgatacatg
     6241 aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt agaagagaag ttgcgacaag
     6301 caaatgaaga gattcttcat gcgcatcagt cacaaactca acttctgaaa gaatatgcca
     6361 gcgggacaaa aatagaaatg gaaattatca tggtccatgc acaagatcat ctgatgacga
     6421 cgatgacatt attggaagtt gcacaggaaa tgaattattt gtatcaaaaa aataaataga
     6481 aaggaaggat cattgtgcag gatacagtaa aaaggaaaat cgaattagaa aaaaagcaac
     6541 ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt acgttacctg actgcctttt
     6601 tctttttcat gaacatgcaa tggatgattc tgcttctatc agctaaaagt ttaggaagta
     6661 gtttaccgat gattctgatt ttagctattt tgcctgcagt aggagaacaa gtaaagcttt
     6721 atcgaaaaca ccaaacgaac gttccatgga caaagtggta ttttttgttc cagagtgtct
     6781 ttaatttatt agtgattcca gtgttgttta cctcaggatt tacattcttg tatcctttta
     6841 tggcaaataa caatggagga cagttatttg ttttcattct tgttgtaagc ggtatttttg
     6901 ccagtgcatt tatacagtat cgtttaaaga aaatttcact caaccaagac caacagtata
     6961 tccgtattaa acaatatgaa aaagcgttat atttaggaaa ggagaataac taatgtttga
     7021 ttttttgcag aaatacctga tgggacctat ggggaaaatt gcgcaactta aaatcgttcg
     7081 ggctgtcatg gcagcgggga tggcttctat tcctttcacg attgtaggct cgatgttttt
     7141 ggttttaaat attttgccat taccgttccc atttttagaa ggatttttta atgcaacttt
     7201 tttcaaagtt agtgacttgt acatgatcgt caatacaatg acgatgggga ttttatctat
     7261 ttactttgcc attgtgtttg cttatgaatt aacgtcaatt gaacgagatg aacaaaaatt
     7321 aaatgttaat ccgctcacgg gggcattact atctgttttt gcattcttta tgtgtatccc
     7381 tgaattgatc atgactgatg gtaaaatcag cttgattgct tctatcaatg agacagaaac
     7441 tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt ttaggaactt caggaatctt
     7501 tacagctatt attatgtcga ttattgcggt cgaattgtat tgtatgtgtg tcaagagaaa
     7561 ttgggtcata aaaatgccag atgttgttcc gccaggagtt tctcgttctt tcactgctct
     7621 gatcccaaca tttgtcattg cgtttgtggt gatgatcatc aatggattat tagttgcact
     7681 aggaacggat atcttcaaaa tgattgctat cccatttggg tttgttacgc agttgacgaa
     7741 tacatggctt ggtgtcatgg tgatttattt cttgatccat gcattgtgga tcgtagggat
     7801 ccatggagcg aatatcatca cttctttctt gaccccgatc gtattagcta atatggcagc
     7861 taacgcacaa ggcgcaaatt atccgttggc aggagaattc aacaattctt atgtaaccgt
     7921 tggtggatca ggtgcgactt taggattgat catctttatt gcattcctcg cgaaatctga
     7981 tcagctgaaa gtgttaggaa aagcctcact tgtaccagga ttattcaata tcaatgaacc
     8041 aatcattttc gggatgccaa tcgtctacaa tccctattta gcggttccgt ttttcttagc
     8101 tccgatggct tctgctacac tcgcttattt tgccattcgg ctggaaatcg tcaaaccgat
     8161 gcttgcacag atgccgtggc cttcaccagt cgggatcgga gcatttgttg gtagtggtgg
     8221 tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc atcgcattca ttatctggtt
     8281 cccatttatc aaattctatg ataataaatt attaaatgaa gaaagagaaa aggcagcaga
     8341 attagctagt gaaaagttaa aatgatttct aagaaaaaag gaactgtgac aaaaaatcac
     8401 agtccctttt tttcttagaa atgggaaacc actagtatat aaataccatt attcttcaat
     8461 caaccgcttc acgattgcat aatccccttc ataaggttca tctacaccat tgattttttg
     8521 atagggatcc acgccttttc ccgcaataac gactgaatct tgcggagcgc tttctgctaa
     8581 agcgcgtcga atcgcttcag gacgatccat ttcataatga acgatcaaat ctggattagt
     8641 aattgcttca ttgatttctt ccgcgatttt tttcggatct tcaaatgctg ggtcatctgc
     8701 cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt ccaaaatcat gtcgacggga
     8761 gattgctttg tttcctgggc tgcctaatac aacaattaca cggccatctg gatgttcatt
     8821 tttggcaaat ttcagcaatg ttttcaagct tagataatta tgtgcatagt ccacatagac
     8881 atgcgctcca ttggattgga tcaattgatc cattcggcct ggtacacgag cttcttttaa
     8941 tccgtcttgt gcatcttgac tggaggcacc tgttagtgct gcggcaatga tcgcactggc
     9001 tgcattttct tggttgaatc ctccagccag caaaatatca taagcagtat cgcagatagc
     9061 taagcgatct tcatgagaag ataaagtaaa accgtgagta cctttatctg aacggatcac
     9121 ttgataattg ctttcttccg aacgaccata agtgatcgtt ggaatctgga atagatcggc
     9181 tgtttcccta agtaaatcaa aatagtcact ttcatgattt aaaacaactg tttttgaatt
     9241 caagatcaat tgtcgcttgc aatagaaata gtcatcaaat gtcggatgtt cgatcggact
     9301 gatatggtca ggcgagatat tcaagaagat tccgacatct aaagtcaagc cgtatacgcg
     9361 ttgtgttttg taagcttgtg aagaaacttc catcactaga tgagtcatac cattttcaac
     9421 tgcttctgac atcatacgat ataaatctaa tgactcaggc gttgtcaaat gggatttgaa
     9481 atatgttttg ccatctaacg ttgtattcat agtagaaagc aatgctgttt ttttatttgt
     9541 tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt gttttacctt ttgttccagt
     9601 gaatccgatc actttcaatt tattttgcgg atagtcgtaa aaagccatag caagaagcgc
     9661 cattgcttta cggatatccg ttacgatgat tcctactgct gaattttcgt atggttgttc
     9721 tgatacataa tattgaatac cctgttcgat tgctgaatcc aaatattctt ctttgaaatt
     9781 cattccttta cagaaaaaca aagtagaagc atcagctttt cttgaatcat aacttaactg
     9841 aagaaattca gctttttctg gtacatcaag atgccagcct cttgttgaaa caaattcttt
     9901 taatagattt tcttttaata gtaaatgacg gatgtcttct aaagagaagc tcattgattg
     9961 atccctcgct ttattcaatt tcttggttta ttatagcaca ctctttttaa tatatcgttt
    10021 gtttccgcta tgacaaaatc cagctttatt caaattttct tgacacaggg gacaagcgga
    10081 tgagtatttt cataaacaat gaactgactg taatagatag gagaacgaat agtgtctcca
    10141 acaatccaaa aacataataa ttcaaaaaga cgataagact attggtaagt aaaagtcctt
    10201 gcggcattgg aagccgaaaa aatttgtgga aaataatcgg cggtatcgtt ataccgccat
    10261 ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact gaacaaaaga cttcctgaaa
    10321 tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg aacaatctct aaaacaacag
    10381 gaaaaagcag acttcccgct acagtcttaa aaaatactga ttttcctaat aggacagcgg
    10441 ctagaagcag catcataaga ttgatgctcc aaacagtaac tgagcgttgg atagaaaagg
    10501 ccgtttcaac taaatgtccg atggcgccca ctccggcgga agctatatcg tgagggagca
    10561 agaaccaatt caatccaact gtgataaaga aaagacctaa aaatataacg ggtagttttt
    10621 ttatttcttg tgtcattgat atctcctata aatgttagcc ataaaaggct gtatatttct
    10681 ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa ttgttcttat aaaagttata
    10741 taaaatcgat tgccagtttc tgttttacca attaaaatga tagtaatgtt ttgtagaaag
    10801 ttggaatctg accgatggat aatcaaagaa aaccagcatt agatcaatta acaccgcaag
    10861 aaaaaatggc acgaggctct gcttggatga ccgccagtaa tatcatttca agattgctag
    10921 gcgctgtcta tatcattccc tggtatgctt ggatgggcga aaatgctaag gctgccaatg
    10981 gattattcaa tatgggatac aatatatatg cattgtttct actgatctct acagcaggaa
    11041 tacctgctgc gattgcaaaa caaactgcac gctataattc attgaatgaa tatggcactt
    11101 cacgacgatt atttatccgt gcattacaga tgatgggcgg gttaggcctg ttgtttgctt
    11161 tgttcatgta catcgcctct ccttggctag ctcatgcttc tggtggcgga gaagaactga
    11221 ttccaacgat gcgttctttg agtatcgctg tattggtttt tccttgtatg agtgtgatac
    11281 gagggtattt gcaaggcaat caagaaatga tgccttatgc gctgtcgcag attgtagaac
    11341 aggtggcacg tattttttat atgctattgg ctacattcat catcatgaaa gtcatggatg
    11401 gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc atttatcggt atgctagcaa
    11461 gtattcttgt ccttttgtat tttttgaaga aacatcaagc gtatacatcg gcgtttattc
    11521 actacagcga aaacaaaata aatattgcaa cgaaggaatt gttgttagat accgttaaag
    11581 aagcgattcc gtttattatt gtgggttcag gggtaacgat cttcaaatta gtcgatcagt
    11641 tcacttttat gaagatcatg tctgcatcca ccgagtattc caatgcacag ttactggatc
    11701 ttttttcgat ttttagtgcc aatccagata agttgacgat ggtagttatt gcattggcaa
    11761 catcgatcgc gtctactggg ttaccgttga ttacagaagc tgttacgttg aaagatcgag
    11821 taggtctggc aaaattgaca agcggaaatt tacagttgtt ttcatttttt atgtttccgg
    11881 cgacatttgg tgttatgtta cttgcctatc cgctaaatac attattttat acaccggatt
    11941 ctctaggaag taatgtactg atccaagcaa gttttgtagg actcttccta ggactatata
    12001 tgctagtttc caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg
    12061 ttattgggtt tctagtgaaa ctccttcttc aatacccagc cattcgttta tttgaagtat
    12121 acggcccttt attggcgaca atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga
    12181 gaatccatga agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct
    12241 ttattcttac cttgattatg cttttggctt cgtggatcac aaagatgatt tttggtgctt
    12301 ttttaaacga agatagcaaa ttccagtctc tattgttgat tcttgtagta gcaggtatag
    12361 gggctttagt gtatggttat ttagctttga aaatccgctt ggcagaaaga ctactaggac
    12421 caggaatagc gcgtttacga cataaattaa aaataaaata agtgaatcag aagagctaga
    12481 ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga taagttttta gcagatatgg
    12541 gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa aggaacgatc gaagtcaatg
    12601 gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg gttacttatt
    12661 taggagaaat cattacgtat caaaaagatt tttactacat tttgcataaa cctgcaggag
    12721 tcatttctgc tactcaggat aatcatgatc aaaccgtaat cgatttattg gaagaccagg
    12781 attatcggga agatcttttt ccagtcggcc gattagataa agacacagag ggcctcttga
    12841 ttttaacgaa tgacggggtg tttgcccatc agttactatc acctaaaaaa catgtggaga
    12901 aagaatatct agctgaggtt agcggtataa tgacagaaga agatatccgt ttatttgcag
    12961 aaggactgat tattgatggc gatgagcaga cattgccggc aaaactccaa attgatcaag
    13021 tagatcaaga gacagaaact tcaattatcc gtctgatttt gcatgaagga aagtttcacc
    13081 aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa agaatccgta
    13141 tgggaaaatt ttggctgcct aaagatttga ataaagggca atatcgaccc atgaccaaag
    13201 aagagctaga acaagtgaga gaaaaataat tgaatggata gctaaaagaa taagcagagt
    13261 agttgtagcg ccatctgaaa agcagacttg tccgtctgac tttcggatgg cttttttgtg
    13321 aaatttcggc ttatttccgt agaagctact tctggaacac cgtttgttcg gaataaggga
    13381 ctgtgacaag acttttgtta cagtccccat tgttccttgt gactcagagt tagatagtaa
    13441 ggacacgatc tccctaatac tcaggccaaa acgctgaatt cacaacctct gataaacggt
    13501 gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    13561 cccgtttttt tctctaatac tcaggccaaa acgctgattt cacaacctct cctatacttt
    13621 gtttcatttt ccctatactt tgtttattta ttctcattat tgaaagcgct ataattacgt
    13681 tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa tcattttttt
    13741 atgaatctat ggcgttataa agcacttgtc ctgatggctg taccgggtat gctttggatg
    13801 atttttttct tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa ttttcatatt
    13861 tcagcagatg ggttcttggc tagcttgagg gaaagtcctt gggtgggttg ggaaaatttc
    13921 aaatttttat tttcttcgga tcaagcattt ctcatcacta aaaatacaat tttatataat
    13981 gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag
    14041 ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta
    14101 tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc
    14161 aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat
    14221 tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt
    14281 tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga
    14341 gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg
    14401 attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac
    14461 attcctagga attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac
    14521 aatggcctga catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca
    14581 gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat
    14641 tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc
    14701 gctctttcaa taagacggct aattttttct ctaatataat gattgcactc tttgctattt
    14761 cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt
    14821 cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt
    14881 tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt
    14941 taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa
    15001 actttccatt ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg ttctcaccag
    15061 ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg
    15121 cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta
    15181 aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc
    15241 gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat
    15301 ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata
    15361 agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac
    15421 aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg
    15481 ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc
    15541 agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata
    15601 taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt
    15661 gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag
    15721 gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat
    15781 ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc
    15841 aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc
    15901 ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc
    15961 tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat
    16021 gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga
    16081 aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt
    16141 tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa
    16201 aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt
    16261 gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa
    16321 atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg
    16381 tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa
    16441 ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt
    16501 ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct
    16561 acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa
    16621 tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg
    16681 gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta
    16741 gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta
    16801 tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat
    16861 gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta
    16921 agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat
    16981 ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta
    17041 ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca
    17101 aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag
    17161 ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat
    17221 ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat
    17281 cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt
    17341 gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa
    17401 gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta
    17461 tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg
    17521 ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg
    17581 gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt
    17641 taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt
    17701 tatatttatc tgttcaaatc aaagggctca tttt
//
LOCUS       DACDEA010000052        17728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDEA010000052 DACDEA010000000
VERSION     DACDEA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.052"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(195..560)
                     /locus_tag="IV136_001885"
     CDS             complement(195..560)
                     /locus_tag="IV136_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465866.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            768..1250
                     /locus_tag="IV136_001886"
     CDS             768..1250
                     /locus_tag="IV136_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465867.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1447..2337
                     /gene="pstS"
                     /locus_tag="IV136_001887"
     CDS             1447..2337
                     /gene="pstS"
                     /locus_tag="IV136_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8465868.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2624..3898)
                     /locus_tag="IV136_001888"
     CDS             complement(2624..3898)
                     /locus_tag="IV136_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8465869.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4171..6642
                     /locus_tag="IV136_001889"
     CDS             4171..6642
                     /locus_tag="IV136_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8465870.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6745..8481)
                     /locus_tag="IV136_001890"
     CDS             complement(6745..8481)
                     /locus_tag="IV136_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8465871.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8478..9182)
                     /locus_tag="IV136_001891"
     CDS             complement(8478..9182)
                     /locus_tag="IV136_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465872.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9313..9816
                     /locus_tag="IV136_001892"
     CDS             9313..9816
                     /locus_tag="IV136_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8465873.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9776..10114
                     /locus_tag="IV136_001893"
     CDS             9776..10114
                     /locus_tag="IV136_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8465874.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10135..11553
                     /gene="ffh"
                     /locus_tag="IV136_001894"
     CDS             10135..11553
                     /gene="ffh"
                     /locus_tag="IV136_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8465875.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11665..12243
                     /locus_tag="IV136_001895"
     CDS             11665..12243
                     /locus_tag="IV136_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8465876.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12247..12768
                     /locus_tag="IV136_001896"
     CDS             12247..12768
                     /locus_tag="IV136_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8465877.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12847..13401)
                     /locus_tag="IV136_001897"
     CDS             complement(12847..13401)
                     /locus_tag="IV136_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8465878.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13654..13929
                     /gene="rpsP"
                     /locus_tag="IV136_001898"
     CDS             13654..13929
                     /gene="rpsP"
                     /locus_tag="IV136_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8465879.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13941..14192
                     /locus_tag="IV136_001899"
     CDS             13941..14192
                     /locus_tag="IV136_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8465880.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14317..15108
                     /locus_tag="IV136_001900"
     CDS             14317..15108
                     /locus_tag="IV136_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465881.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15278..15799
                     /gene="rimM"
                     /locus_tag="IV136_001901"
     CDS             15278..15799
                     /gene="rimM"
                     /locus_tag="IV136_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8465882.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15789..16550
                     /gene="trmD"
                     /locus_tag="IV136_001902"
     CDS             15789..16550
                     /gene="trmD"
                     /locus_tag="IV136_001902"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8465883.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16686..17033
                     /gene="rplS"
                     /locus_tag="IV136_001903"
     CDS             16686..17033
                     /gene="rplS"
                     /locus_tag="IV136_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8465884.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17165..>17728)
                     /locus_tag="IV136_001904"
     CDS             complement(17165..>17728)
                     /locus_tag="IV136_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8465885.1"
                     /translation="VDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNAS
                     VVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQT
                     TKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHE
                     NSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5807 a         3190 c         3615 g         5116 t
ORIGIN      
        1 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
       61 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      121 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      181 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      241 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      301 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      361 acagtagata taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa
      421 tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga
      481 aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt
      541 ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt
      601 ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga
      661 ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag
      721 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
      781 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
      841 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
      901 ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata agtgtaggtc
      961 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1021 tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1081 tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg acgatcaaac
     1141 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1201 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1261 tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1321 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1381 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1441 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1501 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1561 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     1621 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     1681 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     1741 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     1801 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     1861 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     1921 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     1981 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2041 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2101 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2161 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2221 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2281 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2341 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2401 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2461 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2521 ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg tgaagacaaa tttgaataaa
     2581 acttagtccg atatgcggcg cgttctgtgc gccgcatatt tttttagttt tcaaagatca
     2641 gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa ccaaacgcaa
     2701 ttcgtttaat cagcttgatc ttgttattca gcccctcagt gacgccgttt gagtgggaca
     2761 ccttaaaggc atttgaaata cccgattggt agcaatggaa gatcgtgaat ttctttttaa
     2821 acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct tccgtacctc
     2881 gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc aattcttcgt
     2941 tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta aacagtcgat
     3001 gataatgccg tttgagtggc tcccatgtcg tggaatcttt gagtaagagt ttccaatatc
     3061 gcttgagccg acggtacttc ttctgatttt cagagtgtga ggttcgaaaa cgattcatga
     3121 tctggacacg taattgattg aagctgcgat tgatgtgttg gacaatatga aagcggtcag
     3181 ttacgatctc cgcatttgga aagactgttt taagcaactg acaatagctg gcattcatgt
     3241 ccatcactag gtacttgacc tttaaacggg cctttctagg aaagcgcagg aagtattgag
     3301 ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgattggtt gtcccatcaa
     3361 cacaaataaa actcatggaa ccagaaacag aattcattga tttgaactca tccatacaca
     3421 acaccgtcgg aaggaagttc atacgtggct ggtaatgctt cgttgcttca cgcaagatcc
     3481 gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt gattgatttt
     3541 cgtacagttc catgagaatc gcctgcttca gttcctttgt tagttgatga tgttcatcaa
     3601 ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa cgggtacgtt
     3661 ttaattctaa aatagttaac ttttctttga ctttcaacat ctgagtgcgt gtcgtatagg
     3721 aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagaa gagtcatcca
     3781 attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc agccattctt
     3841 catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga ttatccattg
     3901 agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa gtgagtctct
     3961 ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca
     4021 caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc
     4081 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
     4141 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
     4201 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
     4261 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
     4321 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
     4381 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
     4441 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
     4501 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac
     4561 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
     4621 actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag
     4681 gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc
     4741 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
     4801 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
     4861 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
     4921 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     4981 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     5041 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     5101 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     5161 aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca
     5221 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     5281 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     5341 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     5401 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     5461 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     5521 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     5581 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     5641 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     5701 aatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     5761 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag
     5821 cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa
     5881 gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg
     5941 gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt
     6001 gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg
     6061 ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca
     6121 ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa
     6181 aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta
     6241 acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa
     6301 acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat
     6361 aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt
     6421 aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta
     6481 tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc
     6541 acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc
     6601 gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa
     6661 agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta
     6721 aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc
     6781 tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac
     6841 aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta
     6901 aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac
     6961 agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga
     7021 atattggaca gcattttcaa tcagattttt gataattgga taaaggatat ctgttttagt
     7081 agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg
     7141 ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta
     7201 agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat
     7261 cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc
     7321 atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga
     7381 gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata
     7441 cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc
     7501 taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt
     7561 cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc
     7621 aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg
     7681 agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa
     7741 ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt
     7801 aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt
     7861 aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg
     7921 atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa
     7981 gacccaacgg cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt ctgcgatacg
     8041 taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc
     8101 attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt
     8161 gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat
     8221 ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa
     8281 agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg
     8341 tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga
     8401 aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc
     8461 ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata
     8521 gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg
     8581 tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat
     8641 gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct
     8701 ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca
     8761 gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt
     8821 tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg
     8881 gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta
     8941 gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc
     9001 atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat
     9061 gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc
     9121 aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc
     9181 attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa
     9241 agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag
     9301 gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat
     9361 ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg
     9421 gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt
     9481 ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat
     9541 gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac
     9601 attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac
     9661 ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag
     9721 caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga
     9781 aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga
     9841 aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc
     9901 ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat
     9961 cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt
    10021 attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa aactacttga
    10081 atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca
    10141 tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa
    10201 gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc
    10261 gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg
    10321 gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa
    10381 ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact
    10441 gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc
    10501 aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga
    10561 ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat
    10621 atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa
    10681 aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg
    10741 atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc
    10801 gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt
    10861 attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg
    10921 atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac
    10981 ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg
    11041 acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag
    11101 aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg
    11161 ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga
    11221 atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct
    11281 atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt
    11341 gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa
    11401 tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa
    11461 atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag
    11521 aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac
    11581 tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt
    11641 actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg
    11701 cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga
    11761 aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt
    11821 gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa
    11881 acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa
    11941 gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca
    12001 gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa
    12061 gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga
    12121 tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat
    12181 gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa
    12241 taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg
    12301 aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa
    12361 gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa
    12421 aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac
    12481 gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa
    12541 aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa
    12601 ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta
    12661 ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac
    12721 acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa
    12781 taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta
    12841 tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa
    12901 gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc
    12961 gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag
    13021 atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg
    13081 tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa
    13141 tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct
    13201 cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc
    13261 attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa
    13321 tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa
    13381 aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac
    13441 aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag
    13501 attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat
    13561 ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc
    13621 ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt
    13681 atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt
    13741 gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta
    13801 gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact
    13861 gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca
    13921 aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc
    13981 cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg
    14041 agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg
    14101 ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc
    14161 gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag
    14221 tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga
    14281 aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt
    14341 ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat
    14401 ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct
    14461 tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag
    14521 ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat
    14581 ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat
    14641 cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta
    14701 tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc
    14761 gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca
    14821 gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat
    14881 aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa
    14941 aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg
    15001 aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa
    15061 gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct
    15121 gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat
    15181 ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc
    15241 taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg
    15301 aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt
    15361 cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc
    15421 tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga
    15481 aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa
    15541 agacaactct gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca
    15601 tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa
    15661 tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc
    15721 tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact
    15781 gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg
    15841 gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat atctctaact
    15901 tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag
    15961 caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg
    16021 caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga
    16081 aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt cattatgagg
    16141 gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc
    16201 ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag
    16261 atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac
    16321 atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa
    16381 atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc
    16441 aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag
    16501 aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa
    16561 aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt
    16621 aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga
    16681 aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag
    16741 cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac
    16801 gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt
    16861 atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac
    16921 cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact
    16981 acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt
    17041 tatcagacag agaaagaacc cttggtattc aagggttctt tttttattat ctgttttttt
    17101 atattaacga tttctatttg gtacattttt gattaataaa aaggggcaaa aaaggggcaa
    17161 caagttacag tttttctata tcttctttca atttttcttt tactcctttt gtcacatgca
    17221 aataaatttt ttcagtgata tcactatttt cgtgaccaac gcgatcctga atggcataca
    17281 gaggagttcc tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa
    17341 gttttttatc aatcttcatg tcagctttat gacttctcaa ataggtgtta attgctgtta
    17401 gttggaaagg agttcctttc gttgtttgaa atagaaattg accatttgga tttaattcta
    17461 gaagctcatt gtaaatagct atcgcttttt ttggtaaatc aatttctcgc attccggcag
    17521 ctgtttttgt agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg
    17581 tcccatttat aactactgat gcattattat tggtaatgtg tacatcatct ttacttaatg
    17641 cgattgcttc accagacctc atcccagtca aatatagcca ctggaaaagg agagaatatc
    17701 ttttattgtg tgaagttgtg aaatctac
//
LOCUS       DACDEA010000053        17482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDEA010000053 DACDEA010000000
VERSION     DACDEA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.053"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IV136_001905"
     CDS             complement(59..187)
                     /locus_tag="IV136_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8465886.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV136_001906"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV136_001906"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8465887.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IV136_001907"
     CDS             complement(1674..2729)
                     /locus_tag="IV136_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8465888.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV136_001908"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV136_001908"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8465889.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV136_001909"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV136_001909"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8465890.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV136_001910"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV136_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8465891.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV136_001911"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV136_001911"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8465892.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV136_001912"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV136_001912"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8465893.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IV136_001913"
     CDS             10491..12050
                     /locus_tag="IV136_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8465894.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IV136_001914"
     CDS             complement(12161..14092)
                     /locus_tag="IV136_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8465895.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV136_001915"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV136_001915"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8465896.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IV136_001916"
     CDS             complement(15021..15773)
                     /locus_tag="IV136_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8465897.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IV136_001917"
     CDS             complement(15932..16141)
                     /locus_tag="IV136_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465898.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IV136_001918"
     CDS             complement(16152..17480)
                     /locus_tag="IV136_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465899.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         4580 a         3783 c         3169 g         5950 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tt
//
LOCUS       DACDEA010000054        16940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDEA010000054 DACDEA010000000
VERSION     DACDEA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.054"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(301..663)
                     /locus_tag="IV136_001919"
     CDS             complement(301..663)
                     /locus_tag="IV136_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8465900.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            886..1638
                     /locus_tag="IV136_001920"
     CDS             886..1638
                     /locus_tag="IV136_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8465901.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1778..2701)
                     /locus_tag="IV136_001921"
     CDS             complement(1778..2701)
                     /locus_tag="IV136_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8465902.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2716..2988)
                     /locus_tag="IV136_001922"
     CDS             complement(2716..2988)
                     /locus_tag="IV136_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465903.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(3458..5083)
                     /gene="groL"
                     /locus_tag="IV136_001923"
     CDS             complement(3458..5083)
                     /gene="groL"
                     /locus_tag="IV136_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8465904.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(5134..5418)
                     /locus_tag="IV136_001924"
     CDS             complement(5134..5418)
                     /locus_tag="IV136_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8465905.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            5643..6305
                     /locus_tag="IV136_001925"
     CDS             5643..6305
                     /locus_tag="IV136_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8465906.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(6381..7673)
                     /locus_tag="IV136_001926"
     CDS             complement(6381..7673)
                     /locus_tag="IV136_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8465907.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            7843..8472
                     /locus_tag="IV136_001927"
     CDS             7843..8472
                     /locus_tag="IV136_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8465908.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(8575..9384)
                     /locus_tag="IV136_001928"
     CDS             complement(8575..9384)
                     /locus_tag="IV136_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8465909.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9439..10308)
                     /locus_tag="IV136_001929"
     CDS             complement(9439..10308)
                     /locus_tag="IV136_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465910.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(10309..11625)
                     /locus_tag="IV136_001930"
     CDS             complement(10309..11625)
                     /locus_tag="IV136_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465911.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(11622..13457)
                     /gene="walK"
                     /locus_tag="IV136_001931"
     CDS             complement(11622..13457)
                     /gene="walK"
                     /locus_tag="IV136_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8465912.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(13462..14166)
                     /locus_tag="IV136_001932"
     CDS             complement(13462..14166)
                     /locus_tag="IV136_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465913.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(14356..15216)
                     /locus_tag="IV136_001933"
     CDS             complement(14356..15216)
                     /locus_tag="IV136_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8465914.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(15203..15772)
                     /locus_tag="IV136_001934"
     CDS             complement(15203..15772)
                     /locus_tag="IV136_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8465915.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            16153..16225
                     /locus_tag="IV136_001935"
     tRNA            16153..16225
                     /locus_tag="IV136_001935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16186..16188,aa:Lys,seq:ctt)
BASE COUNT         4955 a         3531 c         2857 g         5597 t
ORIGIN      
        1 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
       61 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
      121 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
      181 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
      241 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
      301 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
      361 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
      421 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
      481 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
      541 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
      601 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
      661 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
      721 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
      781 tatcacttaa tagtataaca taatttcatg agaaaaaaat acaatttttt cttgaccatc
      841 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
      901 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
      961 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
     1021 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
     1081 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
     1141 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
     1201 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
     1261 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
     1321 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
     1381 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
     1441 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttatc ttacagctgg
     1501 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
     1561 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
     1621 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
     1681 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
     1741 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
     1801 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
     1861 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
     1921 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
     1981 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
     2041 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
     2101 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
     2161 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
     2221 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
     2281 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
     2341 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
     2401 tctgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
     2461 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
     2521 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
     2581 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
     2641 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
     2701 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
     2761 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
     2821 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
     2881 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
     2941 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
     3001 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
     3061 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat
     3121 tataatgata ctgtaaatat atctttatta ttagtgaatg gatatttact tttaatagat
     3181 aaaatattat aactattaaa aataactatt ttcattataa ttacacacaa aaaagagcgt
     3241 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac agcggaagca
     3301 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca atttccgtga
     3361 atttcttctt atttcttgga gttaaacgct tctgttccag cctcttttta tctgtctttc
     3421 actaatgatc agttgtagat aagcaagttg ctttttctta catcataccg cccatcattg
     3481 atggatccat tgcaggagct gctggcgctg ctggttctgg tttatctgca acgactgctt
     3541 ctgtcgttaa taacaaggca gaaacggaag cggcattttg caaggcagaa cgtgttactt
     3601 tagttgggtc tacgatacct gcttcaacca tgtttaccca ttctccagta gcagcattga
     3661 aaccagtgcc tagttccacg ttcttcaatt tatcaacaat cactgatcct tcataaccag
     3721 cgttttcagc gatttgacga attggttctt ctaaagcacg aacaacgatc ttgatacctg
     3781 ttgccacgtc tccttctgct tctacagcag aaactttgct gataacgttc actagagctg
     3841 taccaccacc tgagaccatt ccttcttcta ctgcggcacg tgtagcattc aatgcatctt
     3901 caatgcgtaa ttttaattct ttcaattctg tttcagtagc tgcaccaact ttaacaacag
     3961 ctacaccacc tgctaattta gctaagcgtt cttgtaattt ttcgcggtca aaatcagaag
     4021 ttgtttcagc aatttggttt ttgatcaatt gcacacgagc ttcgattgct tctttttctc
     4081 cagaaccttc tacgatcgtt gtattgtctt tatctacaac aactttagaa gcatttccta
     4141 agttttcgat tgttgcatct ttcaattcta aacctagatc gtcagtaatt actgtaccac
     4201 ctgtcaaaat agcgatatct tcaagcattg ctttacgacg atcgccgaaa ccaggagctt
     4261 ttacagctac tacgttgaat gttccacgga ttttgttcaa tacaagagtt ggcaatgctt
     4321 caccatctac atcgtcagca ataatcaaca atggacgtga ttgttgcaag atttgttcta
     4381 acaatggcag aatatcttgg atgttagaga tttttttgtc tgtgatcaag atatatgggt
     4441 tttctagaac agcttccatt ttgtcgttat ctgttaccat atattgtgat aagtaaccac
     4501 gatcaaactg cataccttct acaacatcta gttctgtttc gatccctttt gattcttcga
     4561 ttgtgatgac accgtcgttt ccaacttttt ccattgcatc agcaatcaaa tgtccaactt
     4621 tatcagaacc cgaagaaaca gctgctactt gtgcaatggc ttcttttgaa tcaacaacag
     4681 ttgagatatt atgcaattct tcgactgctg cttttgttgc caattcgatc ccgcgacgaa
     4741 tgcctagagg attagcacct gcagttacgt tttttaaacc ttcgcgaacg attgcttgtg
     4801 tcagaacagt tgctgtcgtt gtaccgtctc ctgcaatatc atttgttttt gatgctactt
     4861 ctgatacaag ttttgcaccc atgttttcaa aatgatcttc taattcgatt tcttttgcga
     4921 tcgtcacacc atcatttgtg atcaatggag agccgtaaga tttttctagt acgacatttc
     4981 ggcctttagg tcctaatgtt actttcactg tatctgctaa tttatctacc ccgcgtaaca
     5041 tagctgcgcg tgcgtcttct gcaaatttca attcttttgc catgattgat tcacctcatt
     5101 gattattttt gattattttt ttatcttcca aatttactca acaatcgcca tgatatcttt
     5161 tcctgcgacg atcagatatt cttttccttc gtattttact tctgtaccgg cgtatttttc
     5221 gaacataact tggtcgcctg cttttacagc ggcaggaact ttttcaccgt tttctaacaa
     5281 acggccttca ccgactgcaa caactgtccc agtttggggt ttctctttag cagcagaagc
     5341 taaaacgatt cctccgacag ttttttcttc ttcttttgcg acttcgataa tgacgcgatc
     5401 acctaatggt tttaacacga taaatccctc cgataactct ttttttaatc tgttagcact
     5461 cttaaccttc gagtgctaac ctacattttt aataatactc attctccctc ttttttgcaa
     5521 gcttttaaat caaagaaaag aaattttatt ctgacagctg cttattgaaa ttatgatata
     5581 attgaaatca atcgatataa gcttgccttg catgcttgtt atcccttaaa aggaggaatt
     5641 acatgtcttt aaaaaaatat agcttcctca gcattttttg ctatggtcta atttttatct
     5701 cacccttgat cctagcttcg ataggacttg taaaatctac gtctgaattg atcacagcaa
     5761 ctgcagtcat gtatatacta ggcgccgttg tcctagctat tttttatttt aagcaacgtg
     5821 aacccttagc gatcgaatca gcggtaaaaa aagcttctgc tccaaagatt gttatttatg
     5881 gattggttgg aatctttgtc gcattgattc tgcaaagcat cgctgtcatg ttagaatctg
     5941 tattgtttgg caaagcaacc ccttccgaga atacacaaaa tatcatccag atgatcatgg
     6001 aagcacccgc ttttattttg gcgacaactg ttgctggacc catcatggaa gagttcgtat
     6061 tccgccgcag tatactgggg atcatcagtc gatattcaaa tttttgggtc ggagcagtga
     6121 tcagctcttt gctgtttgcg tttgctcata atgatggtca tctgctaatc tactttttcc
     6181 taggtttctt cttcagccta gagtacaaag caactggtcg gatctggaca tcgatgatca
     6241 cacacgtagg aatgaacaca ttagttgttc ttgttcaatt agctgtccaa aaagggctag
     6301 tataaacatg actataaata ctcctgtcat cctagattgg tgattttccc aatctaaagc
     6361 gacaggagta tttttgtttt ttagtcgttc atcttttctg ctgctccatc attttttgtc
     6421 tgtctttcct ttttgcagtt tgcagaatct atttttttca aaaatgggaa ataaatgaaa
     6481 aaactaattg acaacacgat accttgtaaa atcgctgcag gcactccgcc tactaagaaa
     6541 cccgagagga tcggcggtgt tgtccaaggg acttggactg ctgtaaataa aggaacgaat
     6601 ccaaaatata aagcggaata ggtaatcaat cctgaaacga ccggtgcgaa aataaaagga
     6661 acagccatca tcgggttcat cacgatcggt gtggaaaaaa taattgattc attgatgttg
     6721 aaaaaggctg gtaaaagagc aagatttcct aattctctgt atttcttcga tttcgcaaaa
     6781 accgtcataa aaaatacagc ccctaacgtc aatccagcac ccgtaaccgt catgaactga
     6841 tcgagaaatt gctgagtaac gatatgtccg ccatttgcca ccgtcaactc ttgtcctgag
     6901 gctaagattt ctgcatttgc taatgaattc gcttgcaaga cgggactaac caacccattg
     6961 accatcgcag atccatgaag accaaataac cataaaaacg gaacaaaaaa acctaataaa
     7021 agcacaccaa acaacgtgtc tatgaacgat tgaatgggaa gctggacata tgtccatact
     7081 ccttgaacaa cagtcattct gaacaaatgt tgaaaaaccg ctgaaccgat aagcgtaaaa
     7141 gtgactataa caatgattgg aatcaatgaa gtaaaagact gccaaacatt ttcaggtatc
     7201 ttctctcttt gcttccaccc tttcgcaagt cttactaaac gtgcatagcc ccatccgatg
     7261 atgagcccag ttgtaactgc tcctagtatc ccttcagcac ctgtccagac agtatctatc
     7321 tgagtattta gtggctgaat aagcagtaga taagcagaca aggctgttat gccgactggt
     7381 aagcgatcga tgtcttcttc ttttgcataa gcgtatgcaa tggcaaaaac gatccaaaag
     7441 gctatcacat caaatgtagc ctggtatact tgtgtaaaat acatagaaaa tcctgttttg
     7501 tctatccact gagcgatgta atccacagga aaatgagcaa ataagaaaaa cagcgagcca
     7561 acaataatta ttgggatcgt gtaagtcatt ccattacgca aagcagttaa gggtctaaga
     7621 ttcacaacag atgttatcat tggtaaaaag gcacgatcta accactcttt catttcttat
     7681 ctccttcaac ttctattttt atcaatcagt tttcttaatt taactatacc caatgatcac
     7741 gaaccatgaa aggaatgcgc actgagcttt tcaaaaatcg gattctttga cgaatattca
     7801 gcaactgctt acaatggaca ggaataaaaa aggaggtttt ttatgaaaat tgcactcatt
     7861 ggagctactg gccatgcagg ttcatttatt ttagacgaag cattgtccag agatttagac
     7921 gtgacggcaa tcgtgcgtca tccagaaaaa ttgccatcag atgttccgtt tatacaaaag
     7981 gatttatttg agttgacaac tactgatctt gcatcttttg atgttgtcgt tgatgctttc
     8041 aacgctccca aaggaaaaga agaaatgcat caaacttctt tgtcccattt gattgagatg
     8101 cttgaaggaa ccgatacacg cttaattgtc gttgggggtg cctcttcgtt attcttagat
     8161 acagaaaaga cgactcgtat gattgaccaa gtacctaaaa atgcaccctt ctatccaaca
     8221 gctttcaata tgtacgaagg attgttaaaa ttgaaacaat ctaaaaatct acattggact
     8281 tatatcagtc cagcttcttt atttgatcca catggcaaac gtacaggtac ttatttgtta
     8341 agtgatgatt atttacaaag aaacgctgct agcgacagta cgatcagcat ggcagattac
     8401 gcgattgcat tagtagatga gatactggat gagaaacatg aaaagcagca catcagtgta
     8461 gtcagtcgat aatacggtta tttttttaat atgctaaaaa aataaaaata gaagataaaa
     8521 ccaaccctga ttgattttat cttctgcttt ttatattttt attgattgaa ctgtttaaat
     8581 cgaaaatagc tcagttgctt catctggatc tgtatcatag atttcaaaat catgtccaac
     8641 gccaaaatct ttttctttca aaacattttc cattgttaaa tgagcaagtt ctttcatatt
     8701 gttttccttg ctaagatggc caagatagat tcgtttggtc cgattaccga tcacatcagt
     8761 catgacaagt gcaccgtctt cgttggaaag atgtcctcta tctcctaaaa tacgctgttt
     8821 taaattccac ggataaggtc ccatgcgtaa catctcaaga tcatgattac tttccatcaa
     8881 ataaccatcg gcattttcaa tgacacctcg catataatca ctgcaatatc cggtgtccgt
     8941 caacatgacg aatgaccgat tttctttatg gaaacgataa aattgcggag ccgctgcgtc
     9001 atgggagacg ccgaaactct caatatcgat atcgccgaat gttctgactt tccccatctc
     9061 aaatagatgt ttttgttcca ctggtacatt tccaatcagt ggttccatcg cttcccaagt
     9121 ctgctgattg gcaaaaacat ccaaatgata cttacgagcc aagacaccca cgccatgtat
     9181 atgatctcga tgttcatgcg tcacaagtat tccatctaat tggtccggtg tccgatcaat
     9241 ttgagccatc aaggaagtga tttttttacc gctaagtcct gcatcgacca aaagccgttt
     9301 tttttcactt tcgatataaa gtgaattacc cgaacttcca cttgctagga tactgatttt
     9361 aaaagctggt ttttcttcag ccatttgact cctacttccc ttcttacttt gtaaactttt
     9421 ttcattatac tcgctttttt attgatcttc cacttttggt acagtattgt ttgtcacgat
     9481 cgtattgctg aaagcgttga cgctctcaat ttggaccact ttatcgcttg tttcgatcgc
     9541 tacatgccaa acaggaacat acacattttt ttcccggact ttcagaatac aggaataagc
     9601 tagctgacgc cataaaattt ttgatcctct tggtattttg ttattgatgt acaatgtatt
     9661 gattgcttcc tcttccgtat acagttccgt cttttcgcgc agctgctcga tatcagataa
     9721 atgcgtctgc atgtatttcg tgatgctata atcattatcg tttttttcta aagaaagaac
     9781 gatttttgct gtatcatcat tgaatgggat gccttcatac tcttggcttg cttggatttc
     9841 tagtggttct tcttctaaat gagaaaagtt tttcgtgtac acataatcat taccgaataa
     9901 aatattgtct ttgttagata aaaaatcttc caaggaacta ctaattttat ctggatcgat
     9961 cacgttcttt tcagcatctg aactggataa agatttcgtc aaaacatcac cgtctaaaaa
    10021 cacaccactt gccagcaagc cgcttttacc agccttgcga taattcgcta atgaagtgct
    10081 aagattggtc tgctccgcgc taagataata gccttgcttg ttctcagacg aaatcttccc
    10141 cgtgtaagtg atatcgtcgc tagccagacg ttgttggatt gattcagtct gattagagcg
    10201 aatgacattg ttttcttggt ataccgcgtc atgataactg ctgaataaaa acacattcaa
    10261 ccctaaaaaa gcgacgaaaa atatccattc gatttttttg aaatccaatt aattcgcctc
    10321 caatccaggt aatttttcca gaaggtcgtt agctgaatac cagttatctt tatatttcac
    10381 ataccattca ggtaagagat caacgacacg attcgtttct ttgacatttt gccatgtata
    10441 gccaacgatc actgaaccaa tcttgttgct atctgctccc ttagctttta actgctggat
    10501 gatttcattc gtacttgcaa gttctacttc ttcgtctgaa ggtatcggca cttgaatcgt
    10561 attcaaactg gtttggatat tcacttgcac agaaccattc gtttcaccgc caacttccac
    10621 accaatctgt cctctgctgt ctgtcccaaa aaccgggaag ccttccacga atatccgata
    10681 atcgatcgta ttgtcactgc ggtcaaaata tctgaggttc cctaaagaac tgcctaattt
    10741 gctaatatag gggaaacttt cagaaaatac actagcattt tcttttttct ctggcagctc
    10801 tccacgaaaa tcaactactt gcgtttccga atggatagac attgtttcat tcccaccgta
    10861 aaaaatcaat tcattgcttt catcattatt tttcacgcta tctggtttct ggaaaaaagc
    10921 atttctaaaa agtgtatagg gttgctgtga caggatataa ctatacttct tcagtttgat
    10981 cgaatgtttc gtagcatatt gcgtgtcgat cgtcgatggt tctgttgtca tttctgtcca
    11041 ttttgtgtct tttgctgtta attctttatt gatatctgtc caattcaacg aaatatttgc
    11101 ttcatatacg agatggttcg aataattcaa aaatcggatt tttttatttc cttgatcgaa
    11161 ttgtattcta gtaaaataca tattttcgct ttcttttgcc aagttcgaca catctaaatc
    11221 taaatcaaat gcttccttgt attctcttag caaaaaaggc gcattatagg ccaactcaaa
    11281 accagagttc agatttttca cctgttcaaa ctgtgatttg tctcctgaaa ctacttcaga
    11341 aagctttcca aatcttgctt tatcgatgat caattgcaat cggtacacga gattttcact
    11401 gttggtttct tggatcattc ccgaatggtg ccatgtaaca tgaagcggaa gaaagacttc
    11461 ggaagttttt cgattgcttt gagactcgat catttgagtg tttgtctcat ctacgttgat
    11521 tgaacttttt cctgctgggc tcagccagat tgcataagag aagtatatgc ttaacgcaac
    11581 catcaaaatg agtcctatac gaacgacttt ttcagagatt ctcattccca ccattcctcc
    11641 tcataaggtt catatggcag cgagatataa aaagtcgacc cttttccttc cttgctctct
    11701 gcccaaatag cgcctcgatg cgccttaatc acttctctag aaatagctaa tccaagtcct
    11761 gtcccacctt gtgctcttgc acgtgcttta tctactcgat agaaccgctc gaacactcgg
    11821 ttcagatctt ttttaggaat ccccaatcct tgatcggtga tacttaaaat cacgttatta
    11881 tgcgtttcta ataagcgaca agtaatcgtt cccccatctg gtgaatactt gatcgcgtta
    11941 ttcatgatat tatcgactac ttggatgatt ttatctgtat ctacttcgac ccaaagatct
    12001 ctttgcgtaa attcacgacg gatcttataa tttttttcct gatttcccac catcatatca
    12061 aaccggtcaa gaacaaaatt cactaactcg ttgaaattga catattctaa ttgaagctgg
    12121 gtattacctg tatccatgcg agatagattt aagaggtcat tgatcattcg aatcattcgg
    12181 tctgtttcat ctagcgttac cttcaaaaaa tttggtgcta cttcctcgtc tttccacgca
    12241 ccttcgctca atgcttcgat ataactacgc atacttgtca acggtgtacg aagttcatgg
    12301 gaaacattag agacgaattc acgacgttcc cgttctgttt tttcttgttc tgtcacatca
    12361 tgcaagacag ctactaatcc gctgataaaa cctgattcgc gacggatcat agaaaagtct
    12421 actcgcaaga tcatctgatc actttccaat gtactggttg aacggtcaag taccatttcg
    12481 tttggatctt ctaataattt ccgcaacgta tattcttctt cgatatctaa aagtgtcaaa
    12541 atcgattgtc cgattgcttc ctcgtttttg atatctagca aggaaacagc catatcattg
    12601 atcgtaatga ctttacccct gcgatctgtc gcaatgacgc cgtctgtcat atgggatagc
    12661 acgctatcca aacgattgcg ttcagcttcc attgtatctt gagcttcttc aatacgttca
    12721 gctaactgat tgaaggtatc agccagctgt ccaagttcat cttgtccatg cactttgact
    12781 ttccgactgt agtctcctct agcgattcga atggcttgtt ctctcatttc accgatcggc
    12841 tgtgtaatcg aacgagcaac taaaacagat acgatcattg aaataaatgc cgcaattagt
    12901 gaggcagtaa agaaaatgac tgctgtgtta ttgatttccg aatatttttg ttcgatatta
    12961 cttttgacgt aaagagcccc gataacggca tcgccggtcg gggacaggat cggttgaaca
    13021 ttgatattca cccgtttatc gtcgttatct aaagcaacat aacgtttagt cgtgaaatca
    13081 ttgatatcaa cataatcatt tttctttccc acagcgcttt gttcattgac atcggttgtt
    13141 cctcggatga tccctttttc gtcaacgacc cgtatttcga tgatatcgct agtcgcactg
    13201 ttttcaataa atcttttgat attcgcattt acctcttcat catctgcatt atcttttcga
    13261 ttcaattccg ggctgattgt tgttgctaat gattccaccg tttgattcat atctttaata
    13321 aatgtattga tcgtggaacg ttctaaacca cgtataaagt aggcaccaat aatttcgatc
    13381 gagatcaata aaatcagaat aaaggtaata gcgatcttaa aattgactga tcgaaaaaag
    13441 cggacttttc ttttcatcca attactcctg ttctgggtta cgcaaatagt aaccgacacc
    13501 acgacgtgtc actaaataat ttggatggct tggattatct tcgatctttt cacgcaaacg
    13561 acgtacagtt acatccactg ttcgtacatc tccaaaataa tcataacccc aaaccgtttg
    13621 taataaatgt tctcttgtca tcacttgtcc aaggtgcttc gccaagtaaa agagtagttc
    13681 gaattcacga tgtgttaact cgattgtttc tcctcgtttc gtcaccatgt aagcttctgg
    13741 atggatcgtc aaatcaccaa tcgtcaattc cgcaggtgct gcctcttctg gttccttcgc
    13801 agccgtggca ccgcgacgaa gatttgcttt gacacgtgca actaattctc ggttggaaaa
    13861 tggcttggtc acgtaatcat cggcgcctaa ctctaagcct aggactttat caatttcaga
    13921 atctttggct gttaccataa tgatcggcat atcataagtt ttgcgtacct cacgagcaac
    13981 ttctaagccg tccattttag gtagcatcaa atcaagtaaa atcaaatcag gttctacctc
    14041 agcaactttt tccaaggctt cttccccatc atatgcagta taaacgtcat agccttcttt
    14101 ggctaaattg aatttgacga tatccgaaat cggcttctca tcatcgacaa ctaaaacttt
    14161 tttcataaag agccacctct tatatattat tgtcatacct tctcattata ccgtatttct
    14221 gcttttgttt catctttgcc tataaaacaa aagagaaaat taaaatgaaa gtgcaaaata
    14281 ctttattatt ttttgttcta acttcaataa aaaaggaaaa cactgatttt cacctcagtg
    14341 tttccttttt ttgatttatc cttctaaaac ttgtacaccg acacccaata atcccggacc
    14401 agtgtggact actaatgccg gagaaatctg gccaaagtag atttggtttg cttgtgggaa
    14461 ctctctttta agtctcgctt ccatttcaat tgcttcttct ttcgcatccc catgggcaac
    14521 agctaagttg aagacctttg cattaccgat tttttctttg acgtaagcaa ccgttttatc
    14581 caaacttttt ttacgtcccc tagcttttcc tactgtgtaa taaatccctt cttcattaca
    14641 tgagatgatt gggttaagct ttaaagcggt tcctacaata gaagcaacta aaccgatccg
    14701 tccacctttt tgaagatatt ccaatgtcgc aacattaaag aaaacttttg ttctttcgac
    14761 taactcattc agattactga tcagttgatc ccattctact ccttcttcca gccattttgc
    14821 tgcttggatc gcagaaaaac ctgcaccaat accaatattt ttcgtatcta gtacatatgc
    14881 ttccaattct tcttgctgtt gtgcaattaa acgaaccaca ttatatgtcc cgctcaatcc
    14941 actggaaatt gttaccgcaa gtaccttttc ataaccgtct tttttgattt gctcaaagat
    15001 ttctgtgatc gactcaccat ccggcaaaga tgtaccaggg atttcctctg gcaaacgttg
    15061 atagatctct tcaggggtaa tgtcaatttt atctgtatat acctgatctt tataaataat
    15121 tttcaatggg atcatataca tctcgtattt ttttatcaat tcttctggaa catccgtacc
    15181 tgaatctact aaaattgcga ttttattgtt tttcatttgt tttctcctct tttaaatatt
    15241 cggtttctag aacaatcgtt ttttccgcaa gaagtttcat tgcaaacgat aaagttgccg
    15301 ctcttacagc aatatattct acaggggttg cctggtcgaa tttttgcgga tgagatttac
    15361 cttgtgctaa ttggctgacc atccacacag ctgcttcttg ttcatcgcaa aatagattat
    15421 aggcttcacg aattcctact gtttttcctt ggaacaatat cccttctttg atttcaggaa
    15481 tagtcaatac ttgtttcaac gtagtgattg caagtaaaaa tgctacgtgt tctttgttat
    15541 atcttttttt aactggtgct ggtatcaaac ctagtttaac gtaattgttt accatagaag
    15601 aggtaagcaa tggatgcttt tctccttgta tcacaggtga aagataacga tcaactaacg
    15661 ttctgacttg gtccatataa agttctaagt cagggagttc ttcccagtga ggcagacgga
    15721 atgtttctag ttccttcccc cactccatta attgctgacg cattttttcc atcataaccg
    15781 ctcctttttt catttcataa acatgatatc agacttatga taaacattca atctagttgc
    15841 tttaactagg tgatttgatt agcgggattc catgaaaaca aaaaacaaga aggctaagcc
    15901 ttcttgtctt ttatcgttaa gaacaggaga cccgccgggg agacggcggg aaaggagtaa
    15961 aaaatgaaaa aagtgttagg gtttgttctc ttgcaagaac tttgtttgtt ggtatgctta
    16021 tataatagcg agcaaatgtg aataatctat gaatgatttc ctataaggaa gtaaagattt
    16081 ttctgatttt ctttaaatgt agtcttgcat tttaaaattc ttagtgctac tattattaag
    16141 tactttttgt acgggccgtt agctcagttg gtagagcagc tgactcttaa tcagcgggtc
    16201 gcgggttcga gcccctcacg gcccattggg tgccaaaccc acggaacagg taaatgttgg
    16261 tgcgaaagca tttcgaaaac atttacgctg ttcttttttt atttttcaaa ataactaggg
    16321 ctttttattt ttaactcaaa gtccactgta gccctttgga ctttgagtta aaaaagaagc
    16381 ataattatga acagtaaagt ttctaatata acaataattc gagaagcttt gcaaaatcat
    16441 gctgccaatg aactctctaa acatatagga ttaaatctaa gtactataaa aggttaaaaa
    16501 gtgaagaata tttaatagaa aaactaaatt tacttgctgc tttccccttt tccattaata
    16561 caacaatcag agagtgtctt tttttcgaaa aaattagaat aaataaaaga cacgtagttt
    16621 ttttatctac atgtcttcca tcgactacaa gttatcattt ctcaaaacgt atttagcttt
    16681 taattgatta atcgtttcac gttgtctaca cattgcctaa ttaagatttt cttattagaa
    16741 gcatcaatcc cgcaataata cctaaaactc caggaattac ccacaataat gttaaaagaa
    16801 aaaatggaat aatagataaa atgagtaaac taataccata gaccactgtt ttttgatttt
    16861 ttcttactaa aaaacctatt atgaaaatca ttatcgaaaa aactagggta attcctccaa
    16921 ataaaacaac attcccgctt
//
LOCUS       DACDEA010000055        16748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDEA010000055 DACDEA010000000
VERSION     DACDEA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.055"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV136_001936"
     CDS             complement(451..1761)
                     /locus_tag="IV136_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8465916.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV136_001937"
     CDS             complement(1773..3191)
                     /locus_tag="IV136_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8465917.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IV136_001938"
     CDS             complement(3431..4999)
                     /locus_tag="IV136_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8465918.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IV136_001939"
     CDS             complement(5003..5413)
                     /locus_tag="IV136_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8465919.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IV136_001940"
     CDS             complement(5410..6333)
                     /locus_tag="IV136_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8465920.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IV136_001941"
     CDS             complement(6330..7208)
                     /locus_tag="IV136_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8465921.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IV136_001942"
     CDS             complement(7195..8532)
                     /locus_tag="IV136_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8465922.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IV136_001943"
     CDS             complement(8535..9638)
                     /locus_tag="IV136_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8465923.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IV136_001944"
     CDS             complement(9712..11220)
                     /locus_tag="IV136_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8465924.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IV136_001945"
     CDS             complement(11186..11878)
                     /locus_tag="IV136_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8465925.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IV136_001946"
     CDS             complement(11900..12874)
                     /locus_tag="IV136_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465926.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IV136_001947"
     CDS             13174..14046
                     /locus_tag="IV136_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465927.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IV136_001948"
     CDS             complement(14504..16702)
                     /locus_tag="IV136_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8465928.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4926 a         3507 c         2718 g         5597 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgt
//
LOCUS       DACDEA010000056        16721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDEA010000056 DACDEA010000000
VERSION     DACDEA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.056"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..631
                     /gene="scm"
                     /locus_tag="IV136_001949"
     CDS             <1..631
                     /gene="scm"
                     /locus_tag="IV136_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8465929.1"
                     /translation="SSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTD
                     TTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTD
                     TSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTN
                     KTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(786..1772)
                     /locus_tag="IV136_001950"
     CDS             complement(786..1772)
                     /locus_tag="IV136_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8465930.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1795..2361)
                     /locus_tag="IV136_001951"
     CDS             complement(1795..2361)
                     /locus_tag="IV136_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8465931.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2375..2791)
                     /locus_tag="IV136_001952"
     CDS             complement(2375..2791)
                     /locus_tag="IV136_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8465932.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3248..5182)
                     /locus_tag="IV136_001953"
     CDS             complement(3248..5182)
                     /locus_tag="IV136_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8465933.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5484..6419
                     /locus_tag="IV136_001954"
     CDS             5484..6419
                     /locus_tag="IV136_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8465934.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6509..7612
                     /locus_tag="IV136_001955"
     CDS             6509..7612
                     /locus_tag="IV136_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8465935.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7625..8578
                     /gene="menA"
                     /locus_tag="IV136_001956"
     CDS             7625..8578
                     /gene="menA"
                     /locus_tag="IV136_001956"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8465936.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9513..10859)
                     /gene="gor"
                     /locus_tag="IV136_001957"
     CDS             complement(9513..10859)
                     /gene="gor"
                     /locus_tag="IV136_001957"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8465937.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10993..12234)
                     /locus_tag="IV136_001958"
     CDS             complement(10993..12234)
                     /locus_tag="IV136_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8465938.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12252..13175)
                     /locus_tag="IV136_001959"
     CDS             complement(12252..13175)
                     /locus_tag="IV136_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8465939.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13396..15888)
                     /locus_tag="IV136_001960"
     CDS             complement(13396..15888)
                     /locus_tag="IV136_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8465940.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15895..16362)
                     /locus_tag="IV136_001961"
     CDS             complement(15895..16362)
                     /locus_tag="IV136_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8465941.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16498..16571)
                     /locus_tag="IV136_001962"
     tRNA            complement(16498..16571)
                     /locus_tag="IV136_001962"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16535..16537),aa:Arg,seq:acg)
     gene            complement(16629..16702)
                     /locus_tag="IV136_001963"
     tRNA            complement(16629..16702)
                     /locus_tag="IV136_001963"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16666..16668),aa:Pro,seq:tgg)
BASE COUNT         4943 a         3508 c         2976 g         5294 t
ORIGIN      
        1 ttcaagtgac agtacagata ctacctctac cacaagtgac agcacggaca cgtcagcttc
       61 aagcgacagt acagatacta cctctaccac aagtgatagc acggacacat cagcttcaag
      121 tgacagtaca gatactacct ctaccacaag tgatagcacg gacacgtcag cttcaagcga
      181 cagtacggat acatctacca caacaagtga tagcacggac acgtcagctt caagtgacag
      241 tacagatact acctctacca caagtgatag ctcagattca tctaccacta caagtgatag
      301 cacggacacg tcagcttcaa gtgacagtac agatacatcc tctaccacaa gtgatagctc
      361 agattcatca acttcaagcg atagtacaga cacgtcgtct accacaagcg atacacgtca
      421 tgcaagtgtc tccttctcaa aaacaagttc ggataacagg acgaacttta acttaactaa
      481 taaaacaagc aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa ctggaagtca
      541 atcgaataat tggataacac tcgcaggcgt tatattactt gtaatcggtt taagaatcac
      601 ttttatgagt tataagaaat cgaaaaggta aaaaaatatt caagataaaa aagagcaacg
      661 tcgctaacgg acgttgctct ttatttttgc tttttatcat aaatacaaga actacaattc
      721 aatttgtcaa aaaacacgta gatttccttc tgagaaattt atttacgtat cgaacctttc
      781 cctattcagt ctttgcgatc caaaatcagt tgggtaaggg aaaagagatt ctcttttgtt
      841 cgaagagagg tggcaggtaa tttctttatt tccttcaaag ccttttctgt gtaagaggcc
      901 gctaacttcc tagcttcttc cacgcctcct gtatgttcaa cgattttgcg tacggcgtcg
      961 acatcttctt ctgtcattgc cgctttcttc tctaaataag gtagtagctt tggtttgttt
     1021 ttttgcaaag agtagatcaa cggtaaagaa tagacccctt gacgcatgtc ttctagtaca
     1081 ggtttgccga tttcctggct ctcttttgta taatctaaaa tatcatcgat aatctgaaag
     1141 gccatcccga tagaaagtcc gatatctcct gctttttttg cgaagcgctc gcttgttcca
     1201 ctttcaaaag cgccaacaga acaacttaat tggaacagtt cagctgtctt acctgaaata
     1261 ttttccaaat attcttttac agaaagttca taattatagc gatcatccat ctgccctaat
     1321 tcgccattca atactttttc catactgcga gaattcaatt ggagactttt caaggaagtg
     1381 gagtaatcag acaatagttt aaaacagcag acaaataaat agtctcctgc atagacagaa
     1441 acgacattac caaactgact acggatatta ggctgaccac gtcgcagatc cgcttcatca
     1501 acgatatcat catggatcag tgtggccgta tggagcattt cgatagaagc tgccaaagcg
     1561 atggctttgt ctcgatcttg ctctggtcca aattgtgaga acaatagctg ataggcagga
     1621 cgcaacagct ttccgccggc atggatcgtt tctttgattg cttgttcgac aggtttgttt
     1681 ttcaggcgga cagtttcttc catccgtttt agtgtttctg tcaattcttt acttaaagca
     1741 ggatagtttt cccacattgg atgtagcttc ataaaaaaac tccttgtgtg acgattattt
     1801 tttggtaaac attgggctcc atttttgttt ggttccctgc tcataatctt tttggaatcg
     1861 ttgaagtgtc tgcacatgtc gtaaaagata attcgctgcg atcccgattg caatccctga
     1921 taaaatccct aaaactgaaa ggactggcaa gtacaacatg actgtccatg attgtgcaat
     1981 gaaagaagcc atcaaaagct gtccgacatt atgcatgaaa ccgccggccg cactaattcc
     2041 gatgatactg acacgttttg gtcctaattg cttgaccaat aacattccaa aataactaag
     2101 caaagcacca ctcatactat aaaggaatgt ggaaatcgtt ccgcctagca aagtagtcag
     2161 gaatagtcgt agccaaagta gtaagaagct gtctcgtttt ttcatggtaa aaatggcaac
     2221 gatcgtgatt agattggcta agcctagttt tgcaccaggc gcaaaagaaa aaggataagg
     2281 gatcatattt tccaataaac caatgatcac tccttgtgca acaagtagcg aaatataaat
     2341 cattttttgt aatttactca tggtgtgtga aggtctaata aatcagactg tcttcgtcac
     2401 ctccatctgt tgaacggact tcgatcacta atttatgtgg caaacagacg atggtttcgc
     2461 catttttcgt tgcccagcct cgacgtacgc agatctggtc atcgcaatct gcttctttta
     2521 tccgtactcg atcgccatct atctcgatca agttataatc tccgtgttca tctttgtaag
     2581 tatatgtgtt gctctcttct ccagccatca aagggaatgt tttgatttct gtgccatcca
     2641 cacgcagtac cgcttctttt tctgctgttg cggtgttttg ctgaagacca aaaatcatga
     2701 ctggtaaaaa agaaagaaca gtcaaaagca aaataataaa aacatcccaa ggtttgagca
     2761 gacttttttc agcaaattct ttaaggctca tttttgttct cctttttcca gattaactca
     2821 cccaatttta gcataaaaac aagaggttgt gacacctgtg tcttgctttg agtaataaga
     2881 acgaagaata gaaaacagct ttccatgttt tctaattctt tgggcttatt accgagaagc
     2941 agcaggtgga acaccgttta taacaagagg ttgtgacaga agtgggcaac ttcaagaaat
     3001 aagaaggaat ttacgaaaat tacttttcaa gtttttgtga aatttcggct tatttttcga
     3061 agaagctact tctggaacac cgtttataac aagaggttgt aacagaggtt gtgacacctg
     3121 tgtcttgctc atggaggctt atctcgggca tcttattcct ttaattttac ataaaaagga
     3181 ggctgtgacg gtttgtctca gcccctttct attgaaatct tgcattcaaa taacgttctt
     3241 tatctattta tttcttgccg tactgggaac ttggtctacc gtaccaccaa ttacctagtg
     3301 tacgttgtag ccatggaatt acccagcgat ccaacccaat cgcacgtccg gaaccgttca
     3361 tcaatgcgaa agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg cctgataagc
     3421 agaacgtgat caccaataca atcgttgtcg cactgcatag ccatgtgaat aatccagcaa
     3481 tcaatgctaa tgccaaggca atttcaaaga ataccatgaa tttttggaag aacattgcta
     3541 catcattgtt tggcatgatc acttgcatga tctttgtgaa ccagtctggt gacttctcaa
     3601 tcacagccat tggttcttca ccgtatgcat aactcaaacc aaaatgtgca gcttgagtcg
     3661 ctgcttctcc ggcacttgcc cctgctccgc tggcagctgt ggttgcatca ccagcagcag
     3721 aagcaccact ggcaacatct gttacattgt attcccatgt ccaagggaac acactgtttc
     3781 cagtgaacca tgatccttca ccaaaccaag tacttggttt gaacacttca ccattcccaa
     3841 tgattttttt ggaagcttca acgaaccaaa ctaaaccata gaaaatacga agtggcacac
     3901 tccataagac attgccgtaa cgagaagaat gtcctcgagt cacgttgcga tcatctttga
     3961 tatggaaaaa ctcatgcatt aaatattgga acatataata accagaacga atatcaaaga
     4021 agtatttcaa attcacgata tgtttcatta cgatagctaa aaatccgctt aaatggatct
     4081 tatcaaatag gttagctacc ccccatttgg caccaataga aaccataaat ccttgatagt
     4141 ttcctttaaa tttatgcttt tctccgcctt tgatcgaagc cacgatattc gctgcggctg
     4201 tgtgtcctgt ttgttcagca gcttgaacga tctgcggtgt cggtgttcct gggaattctt
     4261 cataaaagac taaatcacca atgatataga tgtttttatc ttcgtagcct tttgcctgca
     4321 tatattcatt ggcgatcaac cgctgtccgc gtgctgattc taatccaaag tcagcagcgt
     4381 cagaagttcc tttgacacca gctgtccaaa tcagtgtgtg agtcggaatg ttgcgtccgt
     4441 ctttcaattt gatatgatca gcagccactt ctacgattgg tgcattcaat aataattcca
     4501 cgttgtgctt acgcaaatag cgttcagctt ttgcagcatc atttcgatcc aacatattca
     4561 agatcgtcgg cattgcctct acgaccatca gtgtgatttc gtttggatct aatttataat
     4621 ctttggctag gcgatttttc cagtcgatca gttccccaat catttcaata ccggtaaatc
     4681 cagaaccaca gacaacgaaa gtcagcatgg ctttacgcag ttcatcatct ggttccaatg
     4741 cagcttttgc tactgtcttc tcaatatgtt cccgaatcct tacagaatct tcaaatgacc
     4801 aaagggtgaa tccgtgtgct ttgacaccag gtgtaccaaa gtcattcggc tctccgccca
     4861 ttccaaggat cagctgatca aaaggatagg aacctaattt ggtcttgacg atttttcgtt
     4921 ctttatcgat accgattaca gtgtctgtga caagttgtac attcttttga cgagcaaaca
     4981 aatgctgcag atcgtattgg attgcttctg gttctacacg tccgccagct acttcatgca
     5041 actcggtcat catagtatgg tacgaatgac gatcgatcag agtaattgct acatcttctt
     5101 ttttcagttt ctttgccaag tattttgtag ctgaaacacc ggcatatcct gctcctacaa
     5161 cgacaatatt ttgctttgcc attgatcatc cgctccttat agactatcta tataatgaat
     5221 atatgataat tcttttagtt tgatacttat tacacaatct ccatatatta tatagatgtt
     5281 ctgttctttt gtctaactcc ttttggattt ttttcaaatt ttaaaataaa gcgttttttc
     5341 acgaaatttg ctgaaagaaa acgtttgaaa tattacatgg gttatttatc aaaaatagtt
     5401 gacttatagg ctaaaaagat tagaatgtat aatgaatcac aaagaaaaat tgtggactag
     5461 aaatggaatg gtggggtatt aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat
     5521 tagcattttc agctctagtg ttaggcgctt gcggcgcaga tgacaacgga agctcaaact
     5581 cttcttctga atcatctaca gcacaatctt cgactgcaaa aagcacaaca gaatcatctg
     5641 caaaagttgt agctggcgga gatttgcaag acggcacata taaattagaa gaaaaaaatt
     5701 attcaaatgg ttaccgtgca gtctttgaaa tggttgtaaa agacggcaaa atcactgaat
     5761 caaaatatga taacgtcaat gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca
     5821 aaaacatgga agcaaaatct ggcacaaacc cagaaaaata cattcctgag ttgaatgacc
     5881 aattcttaaa agcacaaagc gcaagcggtg tggaagtcgt aactggagct actcactcat
     5941 ctgaaagctt ccaaaactac gcacaacaat tgatccaagc tgctcaagct ggtaacacag
     6001 atacaatcga aatcgacaat ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa
     6061 agaactactc taacggctac cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa
     6121 ctgaatcaaa ctttgattat atcgacaaag acggtaaatc aaaacaagat gatacagaat
     6181 ataatgaaaa tatgaaagca aaaagcggta cagaaccaaa aacttacatc cctactctga
     6241 acgacgaatt cgtcaaagca atgggcgaag aagatggctc acctgctaat gtagaagttg
     6301 ttacaggtgc tacacacagc tcacattcat tcatcatgta cgcacaacaa ttagtgaatg
     6361 cagctgaaaa aggcgacact caaacaatcg aagtagataa tatcgtaaca gaaaaataat
     6421 cacataatca tactaataaa agttggaggg tgggacgata gccagattag gcagtcgtct
     6481 cacctttcaa aatgaagaga ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac
     6541 actgggcatc tcttttatgg tgctaactgg ctgcagcaac tcagcaaata aaacaaccac
     6601 tgaaagttca gcagctacca agatattgaa agatccatat tctgatgaac agttcttgtt
     6661 agggacttac gttcgtatcc gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc
     6721 cttcgatcga gtaaaagaac ttggtgataa aatcacgatc aatcaaaaag gttctgagat
     6781 cgatgaagtg aatgagcaag ctgggatcaa accagtaaaa gtttctgatg acgtctacac
     6841 gttagtcaag cgggcctacg aatatagcca agattctcaa ggcggattcg atatggcgat
     6901 tggtgcaatc acacaattat ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga
     6961 agaaatcgat caggcattga aattagtgga ttatcataag atcgaactaa atgacaaaga
     7021 aaaaactgtt tatctaaaag aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg
     7081 ttacattacg gacgaagtag tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt
     7141 tgacttgggc ggtaatgtct atgtcttagg ccacagccca agaggcgaaa accaagactg
     7201 gacagtcggc atccaagatc cgaacatggc tcgcggatct gttttaggtt ctattaaaga
     7261 acggaacaaa acattagtta cctctggaat ttacgagcgt tatttagaag tagatggcaa
     7321 aaaataccat catttgtttg atccaaaaac gggttatccg ttcgacaatg atatcgcaag
     7381 tgtcacgatc ataacagata aatcaattga tggtgacggg ttgtctacag cagtcttttc
     7441 aatgggtgtg aaaaaaggac tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc
     7501 gatcttcgtg acaaaagacg ataaagtata tgtgaccgat ccaatcaaag acaccttcaa
     7561 attatcggag gactcccatt acacgatggg tgaccgttct gaactaaaat aaggagtacg
     7621 aaccatgaca ctaaaaatct ttttagaagt agttgaaata cgaacaaagg tggctagtgt
     7681 atttcctttt gcggtagggg tattattctc tatcgctttt tttcatgaga tacattggct
     7741 aaacactttt ttatttttta ttggaatgct agtttttgat ttagcgacta ccgctatcaa
     7801 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
     7861 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
     7921 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
     7981 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
     8041 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
     8101 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
     8161 atttgctttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
     8221 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
     8281 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
     8341 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
     8401 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
     8461 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
     8521 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
     8581 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
     8641 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
     8701 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     8761 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     8821 accgtttatc agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     8881 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     8941 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     9001 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     9061 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     9121 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     9181 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     9241 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     9301 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     9361 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     9421 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     9481 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     9541 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     9601 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     9661 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     9721 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     9781 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     9841 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     9901 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     9961 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
    10021 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
    10081 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
    10141 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
    10201 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
    10261 cgttcttttc gatatgccca catgacttca ctacctaaac cacaaaatac acctgaaagt
    10321 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
    10381 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
    10441 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
    10501 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
    10561 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
    10621 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
    10681 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
    10741 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
    10801 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
    10861 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
    10921 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
    10981 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
    11041 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
    11101 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
    11161 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
    11221 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
    11281 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
    11341 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
    11401 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
    11461 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
    11521 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
    11581 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
    11641 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
    11701 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
    11761 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
    11821 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
    11881 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
    11941 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
    12001 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
    12061 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
    12121 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
    12181 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
    12241 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
    12301 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
    12361 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
    12421 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
    12481 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
    12541 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
    12601 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
    12661 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
    12721 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
    12781 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
    12841 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
    12901 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
    12961 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
    13021 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
    13081 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
    13141 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
    13201 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
    13261 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
    13321 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
    13381 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
    13441 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
    13501 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
    13561 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
    13621 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
    13681 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
    13741 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
    13801 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
    13861 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
    13921 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
    13981 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
    14041 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
    14101 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
    14161 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
    14221 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
    14281 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
    14341 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
    14401 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
    14461 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
    14521 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
    14581 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
    14641 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
    14701 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
    14761 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
    14821 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
    14881 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
    14941 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
    15001 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
    15061 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
    15121 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
    15181 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
    15241 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
    15301 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
    15361 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
    15421 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
    15481 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
    15541 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
    15601 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
    15661 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
    15721 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
    15781 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
    15841 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
    15901 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
    15961 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
    16021 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
    16081 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
    16141 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
    16201 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
    16261 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
    16321 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
    16381 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
    16441 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
    16501 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
    16561 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
    16621 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
    16681 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DACDEA010000057        16531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDEA010000057 DACDEA010000000
VERSION     DACDEA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.057"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..184)
                     /locus_tag="IV136_001964"
     CDS             complement(<1..184)
                     /locus_tag="IV136_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8465942.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFF"
     gene            complement(432..1268)
                     /locus_tag="IV136_001965"
     CDS             complement(432..1268)
                     /locus_tag="IV136_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8465943.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1279..2208)
                     /locus_tag="IV136_001966"
     CDS             complement(1279..2208)
                     /locus_tag="IV136_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8465944.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2400..2753
                     /locus_tag="IV136_001967"
     CDS             2400..2753
                     /locus_tag="IV136_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8465945.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2827..3483)
                     /locus_tag="IV136_001968"
     CDS             complement(2827..3483)
                     /locus_tag="IV136_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8465946.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3709..5643
                     /locus_tag="IV136_001969"
     CDS             3709..5643
                     /locus_tag="IV136_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8465947.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5729..6952)
                     /locus_tag="IV136_001970"
     CDS             complement(5729..6952)
                     /locus_tag="IV136_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8465948.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6946..8844)
                     /locus_tag="IV136_001971"
     CDS             complement(6946..8844)
                     /locus_tag="IV136_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8465949.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8838..9956)
                     /locus_tag="IV136_001972"
     CDS             complement(8838..9956)
                     /locus_tag="IV136_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8465950.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10039..10872)
                     /locus_tag="IV136_001973"
     CDS             complement(10039..10872)
                     /locus_tag="IV136_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8465951.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10865..11782)
                     /locus_tag="IV136_001974"
     CDS             complement(10865..11782)
                     /locus_tag="IV136_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8465952.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12009..13133)
                     /locus_tag="IV136_001975"
     CDS             complement(12009..13133)
                     /locus_tag="IV136_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8465953.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13316..14224
                     /locus_tag="IV136_001976"
     CDS             13316..14224
                     /locus_tag="IV136_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8465954.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14365..15978
                     /locus_tag="IV136_001977"
     CDS             14365..15978
                     /locus_tag="IV136_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8465955.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5237 a         3231 c         2743 g         5320 t
ORIGIN      
        1 ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa
       61 cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca
      121 aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta
      181 ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa
      241 catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc
      301 gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca
      361 tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt
      421 attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca
      481 gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg
      541 atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc
      601 gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag
      661 aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt
      721 ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa atagttttca
      781 tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct
      841 aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc
      901 tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc
      961 gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt
     1021 acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt
     1081 gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga
     1141 gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg
     1201 agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac
     1261 cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag
     1321 tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt
     1381 gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact
     1441 taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa
     1501 taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt
     1561 tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt
     1621 atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt
     1681 ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc
     1741 tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac
     1801 accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga
     1861 attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag
     1921 cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg
     1981 tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga
     2041 gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa
     2101 ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa
     2161 gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct
     2221 ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc
     2281 tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt
     2341 tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa
     2401 tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg
     2461 tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg
     2521 aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag
     2581 tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag
     2641 aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac
     2701 tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt
     2761 ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt
     2821 ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat
     2881 caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt
     2941 caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac
     3001 tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt
     3061 gatttgttct gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac
     3121 gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt
     3181 gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg
     3241 atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc
     3301 acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg
     3361 aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct
     3421 taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt
     3481 cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat
     3541 ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt
     3601 atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa
     3661 aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta
     3721 caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta
     3781 gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact
     3841 ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa
     3901 acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg
     3961 aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta
     4021 gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa
     4081 tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt
     4141 cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact
     4201 ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac
     4261 attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag
     4321 aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta
     4381 gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt
     4441 aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat
     4501 gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc
     4561 gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg
     4621 gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct
     4681 ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct
     4741 gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt
     4801 atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa
     4861 gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat
     4921 acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag
     4981 atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct
     5041 ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa
     5101 aattttttga ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta
     5161 gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc
     5221 atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta
     5281 ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg
     5341 gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc
     5401 aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa
     5461 atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac
     5521 ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat
     5581 caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat
     5641 taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta
     5701 tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta ctagtcattc
     5761 tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat
     5821 ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat
     5881 atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg
     5941 ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac
     6001 tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg
     6061 tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt
     6121 tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc
     6181 tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat
     6241 ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg
     6301 ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac
     6361 tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg
     6421 aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa
     6481 gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat
     6541 aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt
     6601 gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag
     6661 gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac
     6721 caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact
     6781 gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat
     6841 tattccattc tttttccatt aattctttat aacattcatt tatccatttt tctggatgta
     6901 ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag
     6961 tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt
     7021 gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt
     7081 tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc
     7141 ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt
     7201 atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt
     7261 gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc
     7321 atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa
     7381 aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac
     7441 ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt
     7501 gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat
     7561 tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg
     7621 actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa
     7681 ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc
     7741 tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc
     7801 atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt
     7861 actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc
     7921 tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt
     7981 gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga
     8041 aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa
     8101 aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg
     8161 cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata
     8221 ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc
     8281 tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat
     8341 aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa
     8401 aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa
     8461 taattgttca tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc
     8521 tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc
     8581 tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca
     8641 taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg
     8701 tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt
     8761 tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt
     8821 attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct
     8881 tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat
     8941 gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt
     9001 tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa
     9061 acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata
     9121 ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga
     9181 ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga
     9241 taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca
     9301 ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg
     9361 atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc
     9421 cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt
     9481 aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa
     9541 tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt
     9601 cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa
     9661 cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt
     9721 tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata
     9781 ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat
     9841 tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc
     9901 ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat
     9961 atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt
    10021 taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga
    10081 ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat
    10141 tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg
    10201 aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact
    10261 tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat
    10321 tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt
    10381 aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg
    10441 gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg
    10501 aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt
    10561 attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc
    10621 tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg gataattata
    10681 tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc
    10741 gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat
    10801 caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt
    10861 ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat ataacagata
    10921 caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg
    10981 ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa
    11041 agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca
    11101 ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg
    11161 ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa
    11221 ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta
    11281 tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat
    11341 tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg
    11401 taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat
    11461 tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga
    11521 taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat
    11581 tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga
    11641 taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat
    11701 aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc
    11761 ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc accagttaat
    11821 tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca
    11881 cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg
    11941 gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt
    12001 tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta
    12061 gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat
    12121 gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata aaaacttgat
    12181 cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg
    12241 cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca
    12301 gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt
    12361 acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta
    12421 ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg
    12481 ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct
    12541 tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca
    12601 cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc
    12661 caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa
    12721 aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga
    12781 aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct
    12841 tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc
    12901 acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca
    12961 acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc
    13021 attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg
    13081 aataataggg taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc
    13141 ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt aacggaatta
    13201 gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt
    13261 tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa ggaaagtggt
    13321 tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa cctattccat
    13381 agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa atgaaagcgc
    13441 taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt cccttttatt
    13501 cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac aaacttttat
    13561 cattatccag ttttattttg atgagcaatt attagcacat attacgggac aatctttttc
    13621 tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt tcaaaattga
    13681 ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt cttttttcat
    13741 actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt gtctgcaaaa
    13801 aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat tgacaatacc
    13861 ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg aattagttga
    13921 tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg aacaattagc
    13981 ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag tgaatggaaa
    14041 aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga
    14101 aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga tccatcattt
    14161 ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc tatccttaaa
    14221 atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc tgtctattca
    14281 aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat
    14341 caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt
    14401 ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa
    14461 cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc
    14521 actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc
    14581 gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt
    14641 aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct
    14701 ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc
    14761 ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa
    14821 catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat
    14881 cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca
    14941 gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa
    15001 acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc
    15061 ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa
    15121 gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc
    15181 aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt
    15241 cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat
    15301 attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc
    15361 tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata
    15421 acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct
    15481 acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa
    15541 gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga
    15601 aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact
    15661 acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta
    15721 gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat
    15781 atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg
    15841 aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc
    15901 agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg
    15961 attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt
    16021 tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata
    16081 gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag
    16141 aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt
    16201 tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat
    16261 tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat
    16321 tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg
    16381 cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt
    16441 tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata
    16501 atttaatttt tttgttccta tttatttaac a
//
LOCUS       DACDEA010000058        15286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDEA010000058 DACDEA010000000
VERSION     DACDEA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.058"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IV136_001978"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IV136_001978"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IV136_001979"
     CDS             complement(580..1353)
                     /locus_tag="IV136_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8465956.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IV136_001980"
     CDS             1497..1847
                     /locus_tag="IV136_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8465957.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IV136_001981"
     CDS             1828..2544
                     /locus_tag="IV136_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8465958.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IV136_001982"
     CDS             2544..3992
                     /locus_tag="IV136_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8465959.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IV136_001983"
     CDS             complement(4062..4571)
                     /locus_tag="IV136_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465960.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IV136_001984"
     CDS             complement(4561..5286)
                     /locus_tag="IV136_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465961.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="IV136_001985"
     CDS             complement(5509..7629)
                     /locus_tag="IV136_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8465962.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="IV136_001986"
     CDS             7859..9037
                     /locus_tag="IV136_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8465963.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="IV136_001987"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="IV136_001987"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8465964.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="IV136_001988"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="IV136_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8465965.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="IV136_001989"
     CDS             complement(10390..11643)
                     /locus_tag="IV136_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8465966.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="IV136_001990"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="IV136_001990"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8465967.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="IV136_001991"
     CDS             13221..14567
                     /locus_tag="IV136_001991"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8465968.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="IV136_001992"
     CDS             complement(14875..15261)
                     /locus_tag="IV136_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8465969.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DACDEA010000059        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDEA010000059 DACDEA010000000
VERSION     DACDEA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.059"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IV136_001993"
     CDS             complement(24..722)
                     /locus_tag="IV136_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8465970.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IV136_001994"
     CDS             complement(715..2067)
                     /locus_tag="IV136_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8465971.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IV136_001995"
     CDS             complement(2290..3690)
                     /locus_tag="IV136_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8465972.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IV136_001996"
     CDS             3851..4588
                     /locus_tag="IV136_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8465973.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IV136_001997"
     CDS             4713..5192
                     /locus_tag="IV136_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8465974.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IV136_001998"
     CDS             5189..5956
                     /locus_tag="IV136_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465975.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IV136_001999"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IV136_001999"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8465976.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IV136_002000"
     CDS             complement(6859..7461)
                     /locus_tag="IV136_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8465977.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IV136_002001"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IV136_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IV136_002002"
     CDS             8142..8726
                     /locus_tag="IV136_002002"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8465978.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IV136_002003"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IV136_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8465979.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IV136_002004"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IV136_002004"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8465980.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IV136_002005"
     CDS             10627..10806
                     /locus_tag="IV136_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465981.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IV136_002006"
     CDS             10828..11853
                     /locus_tag="IV136_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8465982.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IV136_002007"
     CDS             12028..13272
                     /locus_tag="IV136_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8465983.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IV136_002008"
     CDS             13425..14372
                     /locus_tag="IV136_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8465984.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IV136_002009"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IV136_002009"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8465985.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACDEA010000060        15149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDEA010000060 DACDEA010000000
VERSION     DACDEA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.060"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..74
                     /locus_tag="IV136_002010"
     CDS             <1..74
                     /locus_tag="IV136_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465986.1"
                     /translation="ADIILELSGKKLKKVNKMNLEVE"
     gene            74..1450
                     /locus_tag="IV136_002011"
     CDS             74..1450
                     /locus_tag="IV136_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465987.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(1736..3358)
                     /locus_tag="IV136_002012"
     CDS             complement(1736..3358)
                     /locus_tag="IV136_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP8465988.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            4010..4981
                     /locus_tag="IV136_002013"
     CDS             4010..4981
                     /locus_tag="IV136_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8465989.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(5059..5295)
                     /locus_tag="IV136_002014"
     CDS             complement(5059..5295)
                     /locus_tag="IV136_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8465990.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5264..5395)
                     /locus_tag="IV136_002015"
     CDS             complement(5264..5395)
                     /locus_tag="IV136_002015"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8465991.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(5471..5854)
                     /locus_tag="IV136_002016"
     CDS             complement(5471..5854)
                     /locus_tag="IV136_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8465992.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(5887..6672)
                     /locus_tag="IV136_002017"
     CDS             complement(5887..6672)
                     /locus_tag="IV136_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8465993.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6850..7950
                     /locus_tag="IV136_002018"
     CDS             6850..7950
                     /locus_tag="IV136_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8465994.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7940..8764
                     /locus_tag="IV136_002019"
     CDS             7940..8764
                     /locus_tag="IV136_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8465995.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            8770..9576
                     /locus_tag="IV136_002020"
     CDS             8770..9576
                     /locus_tag="IV136_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8465996.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9573..10403
                     /locus_tag="IV136_002021"
     CDS             9573..10403
                     /locus_tag="IV136_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8465997.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10410..11441
                     /locus_tag="IV136_002022"
     CDS             10410..11441
                     /locus_tag="IV136_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8465998.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11548..12821)
                     /locus_tag="IV136_002023"
                     /pseudo
     CDS             complement(11548..12821)
                     /locus_tag="IV136_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13098..13307
                     /locus_tag="IV136_002024"
     CDS             13098..13307
                     /locus_tag="IV136_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8465999.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            13357..14073
                     /locus_tag="IV136_002025"
     CDS             13357..14073
                     /locus_tag="IV136_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8466000.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(13975..14495)
                     /locus_tag="IV136_002026"
                     /pseudo
     CDS             complement(13975..14495)
                     /locus_tag="IV136_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14783..>15149
                     /locus_tag="IV136_002027"
     CDS             14783..>15149
                     /locus_tag="IV136_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8466001.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLE"
BASE COUNT         4509 a         2738 c         3308 g         4594 t
ORIGIN      
        1 ctgcggatat cattttagaa ctaagtggta aaaagttgaa aaaagtaaat aaaatgaatt
       61 tggaggttga ataatgataa aaaatgcatt tgcttatgta actagaaaaa gtttaaaatc
      121 attaattatc atattagtta ttttatctat gtcaacttta agtctcatta gtttatctat
      181 taaagatgct acggacagag cttcaaaaga aacatttgct aatataacaa atagtttttc
      241 tatggagata aatagacaag taaatccagg aacacctaga ggtgggggaa atgtaaaagg
      301 tgaagatatt aaaaagatat ctcaaacaga tagtatagac tcctatgtaa aaagaataaa
      361 cagtgttgca gatttagttg attatgatat tattgaaact caagagactc ttgcgaatca
      421 atcacctgaa agagcaaaga attttaaaag aacagttatg ttaactgggg ttaacgactc
      481 aacaaaagaa acaaaatttg tatcagaagc atataaatta gtagaaggaa agcatttaga
      541 aaatgaagat aaaaataaaa tcttaatgca taaagattta gctgaaaaaa ataaccttaa
      601 agttggagat aagataaaaa taaaatctaa tttatttgat gctgataatg agaaaggtgc
      661 agatgaaaca gtagaagtag aaattaaagg tctattcgat gggcataata gcggtgtagt
      721 aagtgcagca caagaactat acgaaaatac attaatcact gatattcata cagctgctaa
      781 agtttatggt aataccgaag atacagctgt ataccaagat gcaacattct ttgtaaaagg
      841 tgataagaat cttgatagtg taataaaaga tcttgggaaa ttagatataa attggagaga
      901 atataatttg attaaaagtt catcaaatta ccctgcatta caacaatcta tatcaggtat
      961 ttactcaatt tcaaataaat tattttttgg ctcattaata tttgcaggag ttgtagtttc
     1021 attattatta ttcttatgga tgaatgctag aaagaaagaa atagcagtat tactatcatt
     1081 aggtatatca aaattagaaa tttttggtca attcctaatt gagatggtat ttatatcaat
     1141 ccccgcattc gttggttcct atttcttagc tcaatataca gctgacaagc ttgggaataa
     1201 tatattgaat aaggttactg gtgatatagc taaacaaata gctaaacaat ctgcatctag
     1261 ccaattaggt ggtggggcag aagctgaagg atttaacaag acattatcaa gtttagatat
     1321 caatgtatta cctaaattca tgatttatgt agttatattt atgagtttag tacttcttgt
     1381 atcattgatt atttcttcat tcaatatatt aagaaggaat ccaaaagaat tattaattga
     1441 caataattaa aattttcggt gctttttagc accaaaaatg agagtgtaat ataaaccgtg
     1501 taagtaacca tcaatcctag gcgggtggtt gcttcacggt ttttattttt ctaaaataag
     1561 atcaagaggt gattacacga tggctagaaa gaaaagaaat cctgatgctg agaagttagc
     1621 tgaatccatt ttgaatactt atcaacccga atctgtagag gatatgcaag atgttttaaa
     1681 agatgtgttt ggtcccttat ttgaaaaaat gcttcaagga gaattgaata accatttagg
     1741 gtatgacgcc cattctaaag agccaaagaa acatgacaat cgacgaaacg gttatggcaa
     1801 taagacgctt aaaacgagct ttggtaaagt aaacattgat gttcccagag accgggaggc
     1861 ttcttttgaa cctgagttga ttcctaatag aaaacgagat gtctccgaca tcgcagactg
     1921 tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta tctacttcgt
     1981 ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag gtggctaatt
     2041 tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt tccaagcctc
     2101 gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt cgttcaatcg
     2161 tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga tgttccactt
     2221 catccatcag atctccccaa acatgtctcg tgatgactcg ttgatgattt ttacttatgg
     2281 tacattggtt tagcagagga cagttcacac attcttttgg attggatttg tattctcggt
     2341 acccttctcg agtaaccgtt gaaaaagcga gcatttgatc ttgcggacaa atataacaat
     2401 caaaatactc gtcatagaga aactcatttt ttgataatag ctcctttttc cctttgggcc
     2461 ttgaataagg caaacaaggt cgaaggttca aatgagtcaa aaagtgaaca aacttaggtg
     2521 tcttataccc agcatcagcg acaacacaat tgatttcagg aaaaagtctc ttcgattgtt
     2581 tgacgagctg aaaggcgact tgactgtcat gaatatttct aggagtgatg attgtgctaa
     2641 gaataaatcc attgtcgtcg catgaagtat ggacagaata agcaaattgt ttttctcgtt
     2701 cgcctttgac gtagtagccg ctttccggat cagctttact tatttttctt tctttcagct
     2761 tcacttcctt ttctaagtcc aaagtaaaag ggcgttttcc agcctctatt cgttgacgat
     2821 tgatctcatc ttctaattca tcctgatagg ctttcgcttc atcatgggtc atttctttcg
     2881 taaacttatg tttattggcg ttagctttga tgtgggtaga atccagatac aaattatcct
     2941 ctgttacaaa acctgcttta actgcttgtt ctaaaatata ggcaaaaata tcctcaaaca
     3001 ccgtcgtttc tctaaatcgg cgaacataat tctttccaaa agttgagaaa ggggggattt
     3061 tctcctcgaa ggagtagcct aaaaaccagc ggtaagcgac attggtatcg acttccttaa
     3121 tggtttgtcg catggaacgg ataccgaata aatactgaat aaacaccaat ttgatcagaa
     3181 tcactggatc gatacttggg cgacctaaat cggagtaagt ggcttcgacg attggataaa
     3241 taaattcaaa atcaagcgcg gcatcaatct ttcgaacgag atgatcttga ggaactagat
     3301 catcaatcgt aagcatcgca aattgatttc taccattcgt attgttttta gacatcatag
     3361 ctatcactcc ttgcattcgt tttcataaat ctattataaa agaaaagact gtagacatca
     3421 tccataaaat ggactttgtc tacagtctgg ataacgtgat tttttgctgt aatgacaagg
     3481 ttttagctgt ttcagatacc atgtatccaa aaatagttta ttgagaaaat aggagagtat
     3541 ttgatactaa ctagtaggaa aaaatgagat aaaaagtaaa tggaatcatt taaggtaatc
     3601 tgaaataaaa aatatacagc cctgtggtac tttacctaca aggctgtata tattttttac
     3661 tctcagcgtt tttgactccg tgtctgactc cgttttattg attttgaatg ttattcaatg
     3721 accatttaac aggtcaaatt tgatgttttt gattccgtat gacgccgttt gatatctagt
     3781 gatatagtga tttgagtcta catcgaataa gggaataaac caataaaaaa tgatttagaa
     3841 tattaaaata acaaaaaaga ggactacctg tattagtcct cttttttgac cagattttga
     3901 ccagtctatt ttacatccta gtatatttat atgagctaat tttatgatta aatggtaagt
     3961 ttttccttgt gtgttttagt ttaaactagt gtgtaattca aaaatctata tggaaggaac
     4021 agtattaaga atagaacagg gttctctaca tgatggtgct ggtttacgga ccgttgttta
     4081 tttaaagggc tgcccattac gctgtgcctg gtgttcgatt cccgaatcac aaagcaagca
     4141 gattgaaaaa ggatttggcc agaccatgac cgctgaagag gtaatggatg agattgagaa
     4201 ggatgctgtt ttttactttc actcggatgg cggagtgacc attagcggag gcgaggcact
     4261 ggttcaggcg gattttgcta aagaaatttt gcaaaaatcg aaatacattg gaatcaatac
     4321 cgtactggaa accagctttt gtggtgctta taatgaaatc caaaaggtag ctccctatgt
     4381 ggatacattg tttgtggatg taaaaatgtt taccagcaag ctccacaaac aatggaccgg
     4441 cttagataac caacaaattt tgaagaatat tcgtcgcttt ttaattgagt atcctaactg
     4501 cgaagtgcga attcgagttc ctgtggttcc aggtatcaat atgaacctga ccgagctgct
     4561 gacgattgct tgttttgtgg cagatcttga tcggttcgta ccgttagaat tactaccgta
     4621 ccactgctat ggtatgcacg gttaccaagc attgggactg gagtatccgt tggcagacac
     4681 gccagcgcct tcacctaaag aaatgtttgc attggcagat caactggctc gcacggttcc
     4741 tcatttgctg gtcacagctt taaccaaaac atttatttat ttattgaatc caagctttat
     4801 cttatggata aatgaaagac actcactagc tgcagccagc tcaagaatag agcttttgct
     4861 gaagtcagtg agtgtctttt tacgatgttt tttcgaagtt aatttgtcga atgaacactg
     4921 gctcaatcgg tacatcttca ctgtttgtcg gctgagctgc gatcgctgct acgatttcta
     4981 acccttggta tacctgtcca aaaacagtat aacgaccatc cagctccggt agtcctccaa
     5041 gctccaagta tgcttgcttt acattagcag gtcagcctgc gcctttgtgt tttgcacaat
     5101 aaaaaactgg ttgccgttgg tgtgggggcc gtggtcccct aaggccaagg cgccattaaa
     5161 atgataaagt ccgcgatgca cttcatcagc gaaaaagccc tcatagctgg aaacagcttc
     5221 atcaccagaa ccatccaaag cgccaccttg aataacaaat tccttaatga cacgagccga
     5281 aaggtgtgtt gtcataaacg ccttgctcag ctagggagcg ccagttggct acagccaggg
     5341 gagcttggtc aggaaataag cggagacgaa tttcaccagc atcggtgacc agcacacaaa
     5401 cttcttcctg ctgcggtttt tctaattgaa agagaggaag gtttgcaact gaaacgcctt
     5461 gatcctgcca tcaaatatcg aaaatccgcc actcgacctt cgcctgatca ccaaggcgag
     5521 ccattcccaa taaaacggta taggcggttt catccgcttg aaaggtcaat tgcagctgtc
     5581 ctcgtaaatc gttgtagtca tctggggcgg ctacttgata aaaaagatga aactgcgcat
     5641 gcaaatattc caaaggctga tctttaaagg ctgcaaaata agcctgaact ttttcccgct
     5701 gcaagctgtc agccaccaaa aaattttcca gttgttgttt tggaacggct gctaacaagg
     5761 atcggcaaac cgcctcaatt ttcaacgcat cccaatgtcg cgcaattaaa taatggatgg
     5821 gtgcctcttg attgtagtcc cacaggttgc tcattttttt caccactttc ttctattata
     5881 taggaatcac aaaatcggca gcggcgtcag gcggcgttct tttccttgaa tgactagcaa
     5941 cttttctacc tgacagtctg ccaacagctg actcattttt ttaaagccca gctgataatc
     6001 ttcggcaaca tgagcatccg agcctaaggt aaagagcttt ccgccgacac tttgatacaa
     6061 aggaacggca taacggtaaa ggtcttcgtt gccgtatttc aagaagctct tggcatttaa
     6121 ctccatcgcc aattctcgtt caacaacctt tttgaaaatc gttagcagct gagcctcaaa
     6181 atgctccgcg aattcttccg ctgtgaaggt gaagcggcgt aatccataat caaagtgagt
     6241 caaaatatct ccttctggaa aatccgtcaa gacctgcaac atctgatcaa aatattcctt
     6301 ggtagtttga atgggatctt tggttaaaac cacatcgtcc atataatcaa agcggccatt
     6361 ttgatgaata cttaccagct ttaaatcata aggatgctga gcgaggtaat ccacgatccg
     6421 ctcttcttgt cctggaacaa cgccaatctc gatacctcgc agaaatttgg tggaataggt
     6481 ttcttttaaa gagtccagtt tgtttatata ggctggataa tccggaatgt catcttttcc
     6541 ggtagatgga ttgtttaaat caaagtgatc cgtagaaacg aaaaactccg gctgataggc
     6601 taaatagttt tcaaattttt cctctgaatc aaaggaaaaa tacgtgtgga gatgctgatc
     6661 ataatactgc atagggagcc tccttttttc tactatcata ccgcaaaagc tttagaaggt
     6721 taacgggaat tcgtcgaaat ttacaaacca tatacaacct ttacattgtt catgaaacct
     6781 tttcaagaaa ctgattctct cattacaaag ctttgttaga atggaattat cgaaagggga
     6841 gaagaatgaa tgaaaatgaa tgaaaaaatt cgtaacatga ctatcggttt attttgtgca
     6901 gcaacagtaa gtatcctgct gacaggctgc ggctcaggtg aagcaacgaa gcaatcggct
     6961 gcggcagacg actctttaga tacgattata gaaaaagcca aggaagaagg agagattgcc
     7021 agcttgggaa tgccggatac ttgggcaaat tgggtaggca cttggaacga ccttgaaaaa
     7081 gaatacggct taagtcacgt ggatacggat atgtccagtg cagaagaatt ggctaagttt
     7141 gaatcagaag gaaaaaatgg aaccgccgat attggtgatg tcggtatcag cttcggtccg
     7201 ttggctgaaa gcaaagagtt aaccttacct tataagacca actattggga ggaaattccg
     7261 gattgggcaa aggatgatga tggggattgg ttgttaagct atacgggaac catttccttt
     7321 atcaccgata aagagaatgt gaagcaagca ccgactagct ggaaggagct gcaagagggt
     7381 gactatcaag ttagcgtggg ggatgtgacg attgccaacc aagcacaatt tgctgtattg
     7441 gctgcggcga ttgccaacgg aggtgacgaa aaggatattc agccaggtct gaattactac
     7501 aaaaaaatgg cagaaaaagg ccgtttgtcc accgttgatg ccagcgtagc aaatcttgaa
     7561 aaaggtgaaa tcgatgtggc agttgtttgg gacttcaatg gactaaatta tcgtgaccaa
     7621 attgactccg agcgttttga ggtaactgtg ccaacagacg gctccatcat tagcgggtat
     7681 acaaccttaa tcaataaaaa tgcccctcat cccaatgcgg caaagctagc acgggaatat
     7741 attttatctg atgaagggca aatcaatttg gcaaagggct atgcgcgacc tattcgcaag
     7801 gtagagctgc ctaaagaagt agctgaaaaa ttactgccgg aggatgctta tgaagcggcg
     7861 gtgccagttg aagacaatga agcttggaac aagacctctg tagagcttcc agagctttgg
     7921 caggcggagg tactgaccta tgcaaagtag acttgacaaa ctgattgtag ttatactaga
     7981 cggctgtcgc tacgacacag ccgtcgccca gcttgggttc atgaatcatt tagttgagca
     8041 ccagcaagca agcttattga aggtaaaaag tgagctgccc agcaattcac gtccgttata
     8101 cgaagtactg atgaccggcg ttccagccta tgaaaatggc atctatagta attaccatgc
     8161 tcagcgatcc aaagagtggt cgctgtttga attagttaag gaagctggcg gaaaaacagg
     8221 agccgccgct taccactggt acagcgagtt gtacaatcaa gccccctata atcgtttggt
     8281 tgatcgcatc cagcatcagg aggagcgact gattcagcat ggcatctttt atagtgagga
     8341 cacttaccct gattcgcatt tatttgcgga tgcccattat ttagtgaatc agtatcagcc
     8401 agactttttg gtcgtacatt cgatgaatat tgatgatgtc ggtcacaaat ttaccgccga
     8461 ttctcatgaa tatgcagcag cggttaatca ggcggacaga ttattggcta tttaccttcc
     8521 tgaatggctg caaaaaggtt atcaagtcgt ggtgactgcg gatcacggga tggatgcgca
     8581 cggactgcat ggcggcagct tggctgcaca tcgtgaagtt cctttttatt tgatttcaaa
     8641 taaacgccaa aagctgaagg aacaaactgt accacagctg ctggcggctc cattgttttg
     8701 ctggttatta ggaattgcac cgtcagagaa aatgcaggac attcaagaat tgatgagggg
     8761 atgaaagaga tgaaaaaacg cttggttctg gttcctgcct cctttatctt ctgtgcattt
     8821 ttactgctgc cgctgctttt tatggttctg tgcagcttgc acagtgattt aactaaccag
     8881 tggacattgg ccaattacag tgatattttc agcaacaaat attacacaca ggcctttgtt
     8941 aacagcgtac tgattgctgt cagctccagt gtgattgggt taatcgggac gtttatttta
     9001 tgtttatgct taatgaatct atcgactaaa ttgcaggaga aaatcacctt tgtttccaat
     9061 ttggcagcta attttgctgg gattccgctg gcattctctt ttattatttt gttggggaat
     9121 gtaggtgtgc tgaaaatgat tttgccattt ctagcggact tcaatttgta ttcttggtgg
     9181 ggattgacca tcacgtatac ctatttccaa attccgttag gggtcttgtt tttgtatccc
     9241 agcatgaagg aagtgaaaaa ggaatggctg gagtccgcac aattgctggg cgcttcatca
     9301 gcgtatgctt ggttcaaggt ggctttgcct tttttgcgac catcgattgc cagcaccttt
     9361 gtcatcttat ttgctaatgg catggggacc tatgaaacag cgtatgccct gaccagcagt
     9421 aatgtaaatc ttttaacgat tcgaattgcg gctttagtgt cgggagatgt gttcgccaag
     9481 cctaatttag gcagtgcgtt ggcagtcgct ttaggtgtcc tgctggtaac ggcaatgttg
     9541 attattcaaa agaaaggcaa ggtggtgcag acgtgaaaaa gttaataaaa ccgattttgt
     9601 tctgctttgt cctgtattac ttgctgccga ttattggaac gattttgtat gccagctcca
     9661 ctaaatggtc aaaatcgctt ttacctagtg attttacctt gcaatggttc caacagctgc
     9721 tgacggacag ggaatttatt gctgcggtgg gtcgctcact tttattagca ggtgtggtct
     9781 tggtgacgat tctgttgttg atgattccca ccattatttg gattcatttg tatttccctc
     9841 gtttgaatcg ttggctggaa aagctgctcc tgctgccgta tgctttacca ggagtgattt
     9901 tggtaacggc tttgcttcga acgtatgctg aaactggtat cccaatgttt gtagtgttgg
     9961 taggtgcatt gtttattacg gcgttgccga ttgtctatct tagtcttaac aaccagatgc
    10021 gcttaattaa tctgaaggaa ttagtggatg cagcagaaac cttgggcgcc ccgatgtcta
    10081 cgattattat ccaggtgctg tttccaaaca tccgtatcgg tgtgacccta gtttctctga
    10141 tgattttttc ttcggttttt ggggagtaca tgctgacgaa cctgttgatt ggcggtcgat
    10201 ttcagacggt acgcatctat atgctgcggc ggatgaacga aaacggccat ttggctagcg
    10261 ctgtgatggt tttgtatttg ctgtttatgg tgggaattgc tttaactacc tttatgctga
    10321 cttatcgaca aaagcaacag ctgtccctta agcagacgag ggaagagaag cagtcgaaaa
    10381 agaagccctt aaaggagttg tgacggaaaa tgtttttaga aatacatgat ctcaatctgt
    10441 tttttgatga aaaacagatt ttagctattg atgaaattca gattgaaaag ggtgagctgg
    10501 ttactttact aggtcccagc gggtgcggaa agtctacgtt gctgcgctgt atcacgggat
    10561 tggaacaacc aaagagcggt gagattgtgc tggacaatga gaatattgcc gataagccta
    10621 cgaaggatcg caatatcggc tttgtttttc aacagtatgc attgtttcca actatgactg
    10681 tttttgaaaa tgtagccttt ggtttgaagg taaagaagct gagtcctgag gtgattcagg
    10741 aaaaggtttt tgagatgcta tcattggtgg atatgacgga gcaggcagat aaaaatgtcc
    10801 agtttctttc tggcggtcaa cgacaacggg tggcactggc tcgctcattg gtcactgaac
    10861 cgaaagtcct gctgctggat gagccattaa gtgctttgga tgcccgaatt agaaaacagc
    10921 tgcagcggga tttgcgcgct atccagcaat cgctagggat gacgatgatt tttgtgactc
    10981 atgaccaaga agaggcgatg cgaatcagtg atcggatttt tgtgatggaa gctgggcggg
    11041 tggctcaggt ttccacctcg aaggaattgt atcagaatcc tcagagccga tttgttgcgg
    11101 aatttatcgg caactataat cgtttcgaat ggtatgagct gaatcagtat acccacgagt
    11161 tttccgctaa gggcaatcgc tgcatttatt atttaagacc ggagctgatt caatttgaac
    11221 cggttcctca gggggttaag attccggttc tatggaagga atcatttatt ttaggcaaca
    11281 ttcaacggta tgtttttcaa acgctggagg gcaaagaaat tttagtggat cggttgaacg
    11341 atcgggaaga gaaaattagc gatgccttat acattcagct gaaagatatt ctagcaatcc
    11401 ctgtggaaat tgatactcag cattttgcac aacaaagcta gtgtatagtc ggcaaacatt
    11461 tctggctctt tgtcaaatag gactgataga gtgatataag aacatctaag gtagaattga
    11521 ttttctgctt tagatgtttt tttatgttta ataagtgatc agatgattga ttaagaaaat
    11581 tctggttctc atcctaaccc gtaagctatt cgtttcaatg ctttgatgtg ggtatgcaga
    11641 ttttctattt tgccatttga gtaaggcaac tctaaggcta aacgaatgcc ggattcatag
    11701 tttataaacg ctttttttat atgatgcagt tctcttggaa ccgacttggg cattgttttt
    11761 acaagctgaa agaagatgtc cgcttctttg tcataaaaag cttgaagaat ttcttgatag
    11821 taagcataag ctattcgtag ttcatcagaa gtagaaagta gacgttctac gacttcactt
    11881 tcggttaaat aggcccaacc aaaattacgc cattttgtcc atttatcata aacaagcgtg
    11941 tcctgtcgtt tgatcagaag tcgagataaa gccttcactt gtcgatagtg tttggcttgt
    12001 attggatcgt ttcggtcgaa gcttttcatg actcatagtc aaatatcctt aaaactgcga
    12061 atgaggtgct tgacgatgtg aaagcgatcc acaacgagtt tggcatgagg aaagcactct
    12121 tttatcagca aagcataaga ggcgttcata tctgttacga gtatttttac gtttaaacgt
    12181 gcacgtcgtt caaattgcat aaaataagaa atgagatctt tcttcttacg tgaaggaaga
    12241 atatccagta atttcccagt atcgccatcc atacaagaaa aagaataggg accgacttga
    12301 tttttcaatg aacgaaattc atctaccaat gaacagcgag gcaaccaatt tattttcacg
    12361 tttgtcatgg ggagcagaga agcgagtgtt cgcgaaacgg ttgaccaaga aacgccgcaa
    12421 tgtttagaaa tcgtcgtcat tgcttgtttt tcagtcaatt cctccaatat tttataatgt
    12481 acttgtttgg cgataaaaca gtttttttca acaatgtagg tattcgccgt aaaataggtg
    12541 tgacagccac gacagaggta ccgttgcttt gccaaagcaa gataagtccg ttgattcgca
    12601 cacttattga gaaggaccag cgattttttc tttccgttct taacgatcag cccatgttga
    12661 ttcacctgtt tacactgagg acagggctga atcgttttaa tgagcgtggc agaaacaacg
    12721 atactgttga ttgaatgtat ttttttcttc tcgacatgag tgatttgaat gtttccatct
    12781 ttgattccta gtaatagttt gataagatga agatcaggca tacagaacgc tcctttgatt
    12841 gtgttgtggt gattcaattc taacggagct tctgtatgtt ttgttttttt atgctcaaaa
    12901 ggactgatag aatgatttac tatcagtcct agaaattata gcgccacatt tctttcacct
    12961 ttgacttttc agacggcagt ttttatgctg atactgtaga gagagcagga cacagctttt
    13021 gcttgttttg ggtaaggggg aaagaagatg accaaagcac ttatttttga ttttgatggg
    13081 acgatgattg acagtgaatg gatgttttac gagagtattc atcactattt actgaagcct
    13141 atttactgga gcagttcggc tataaaattc cttttgcaga ctatcaacaa ggtgttggga
    13201 cggcagcaga actgtccttt tatgaaggga taagtcaagc ggttgggaga ccagtggatg
    13261 ctgaacattt tgagcagcgg atcagcagca ctggaaaatc ggagtagtct caattctgaa
    13321 ctgcgggagc tagactattt ttttgaagca catccgatga ttaaggaaaa aattgattcc
    13381 tttgtgacga tcgatgatct tcaggtcggc aaaccagatc cagaaggtta tttgttgtgc
    13441 ttgaaacggc tgagggttga acaggctgtt gtagttgagg atagtccagt cggtgcccaa
    13501 gcagcaccgc atatgacagt tgatgtacaa gcgttggatg cagatttttt cgcctttagc
    13561 gggcataaaa tgtgcgggcc gacaggaatc ggtgtattat atggcaaacg tcaattgtta
    13621 gaacaaatgg agccagtcga attcggcggt gaaatgatag attttgtcaa tctttacgat
    13681 agttcatgga aagagctgcc atggaaattt gaagctggta ctccgaacat tgcaggagca
    13741 atcgattact tgaatgagat tggtatggag aatattcatc gttatgagca agaactggtt
    13801 gcctacgttt tacctaaatt acaagcgatc ggcggtatca ccatttacgg accgcaagat
    13861 cctgaacatc atactggcgt ggttgctttt aatttggatg gcatccatcc ccgtgatgtg
    13921 gcgactgctc tggatatgga aggtgtcgct gtacgttcag gacaccattg tgcacaacgg
    13981 ttcatcgacg atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt
    14041 gagcggctcc aatgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg
    14101 gtacttcttc tgattttcag attacgtgat tgattttcgt acagttctat gagaatcgcc
    14161 tgcttcagtt cctttgttag ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag
    14221 gtctgcccac aatcatagca tagaaaacgg gtacgtttta attctaaaat agtcaacttt
    14281 tctttgactt tcaacatctg agtacgtgtc gtataggaac cgttgcggat aatacgaatc
    14341 gatccacagt gttcacaatg ggaaggtgag tcatccaatc tccctttaat aaactgcgct
    14401 tgaacgcctt tatattcttt ttcaatcagc cattcttcat caaaaaataa atgtttatct
    14461 gttaatccga gtaattttct agtatgatta tccatagagc gactcctttg gtagggtgat
    14521 gttttggtcg actaaatttt accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa
    14581 gtgttagtag aaaatcgcct tcgattttcc aacaacacaa aatattatag aagccttttt
    14641 tctaaaggat ttcaaggtgt ctgtttgcat ttattaaaaa aaagaaaaag tattggcaac
    14701 agaaggcgtc accaacacta gatattatag agccataata tgctgtataa tatctagtgt
    14761 ataggcttaa gtggaggtat atatgaaaat attaactgtt gaagatgata atatgataag
    14821 agagggaata agtgaatatc tttcagaatt tggatatact gttattcaag ctaaagatgg
    14881 aagagaagcc ttgtcaaaat ttaatagcga tataaatttg gttatcttag acattcagat
    14941 accttttata aatggtttag aagtgttgaa agaaattaga aagaaaagca atttaccaat
    15001 tctaatcttg actgcattta gtgatgaaga atataaaatt gatgcattta ctaatttagt
    15061 agacggatat gtagaaaagc cattttcatt gccggtctta aaagctagaa tagattcttt
    15121 aattaagaag aattatggac atttagaga
//
LOCUS       DACDEA010000061        14920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDEA010000061 DACDEA010000000
VERSION     DACDEA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.061"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(144..710)
                     /locus_tag="IV136_002028"
     CDS             complement(144..710)
                     /locus_tag="IV136_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466002.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(846..1499)
                     /locus_tag="IV136_002029"
     CDS             complement(846..1499)
                     /locus_tag="IV136_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466003.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1549..1977)
                     /locus_tag="IV136_002030"
     CDS             complement(1549..1977)
                     /locus_tag="IV136_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8466004.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(1982..2356)
                     /locus_tag="IV136_002031"
     CDS             complement(1982..2356)
                     /locus_tag="IV136_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466005.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2660..2845
                     /locus_tag="IV136_002032"
     CDS             2660..2845
                     /locus_tag="IV136_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466006.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2858..3106
                     /locus_tag="IV136_002033"
     CDS             2858..3106
                     /locus_tag="IV136_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466007.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(3409..3867)
                     /locus_tag="IV136_002034"
     CDS             complement(3409..3867)
                     /locus_tag="IV136_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466008.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            4035..4352
                     /locus_tag="IV136_002035"
     CDS             4035..4352
                     /locus_tag="IV136_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466009.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4285..4764
                     /locus_tag="IV136_002036"
     CDS             4285..4764
                     /locus_tag="IV136_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP8466010.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            5214..5645
                     /locus_tag="IV136_002037"
     CDS             5214..5645
                     /locus_tag="IV136_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466011.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            5642..5968
                     /locus_tag="IV136_002038"
     CDS             5642..5968
                     /locus_tag="IV136_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8466012.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            6087..6773
                     /locus_tag="IV136_002039"
     CDS             6087..6773
                     /locus_tag="IV136_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8466013.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            6748..8097
                     /locus_tag="IV136_002040"
     CDS             6748..8097
                     /locus_tag="IV136_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8466014.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            8088..8561
                     /locus_tag="IV136_002041"
     CDS             8088..8561
                     /locus_tag="IV136_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8466015.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            8563..9063
                     /locus_tag="IV136_002042"
     CDS             8563..9063
                     /locus_tag="IV136_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAP8466016.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            9078..11390
                     /locus_tag="IV136_002043"
     CDS             9078..11390
                     /locus_tag="IV136_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8466017.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            11637..11954
                     /locus_tag="IV136_002044"
     CDS             11637..11954
                     /locus_tag="IV136_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAP8466018.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            11951..12112
                     /locus_tag="IV136_002045"
     CDS             11951..12112
                     /locus_tag="IV136_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8466019.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            12109..12414
                     /locus_tag="IV136_002046"
     CDS             12109..12414
                     /locus_tag="IV136_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466020.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            12414..12728
                     /locus_tag="IV136_002047"
     CDS             12414..12728
                     /locus_tag="IV136_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466021.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            12721..13251
                     /locus_tag="IV136_002048"
     CDS             12721..13251
                     /locus_tag="IV136_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8466022.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            13248..13517
                     /locus_tag="IV136_002049"
     CDS             13248..13517
                     /locus_tag="IV136_002049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466023.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            13647..13934
                     /locus_tag="IV136_002050"
     CDS             13647..13934
                     /locus_tag="IV136_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466024.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            14421..14834
                     /locus_tag="IV136_002051"
     CDS             14421..14834
                     /locus_tag="IV136_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8466025.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         5132 a         2479 c         3161 g         4148 t
ORIGIN      
        1 ttttatcagt tttagtttcg atccacattg ttttcatctc ctatttgttg ctataatagg
       61 aatagataag taagccgatt atagcaggtt tatttttcat cacgtccaca aactttggcg
      121 agggaggggc gtgtttttgt tagttattgt ggtaggttat ctaaagcgta ttgtgcttgc
      181 tcatttgtaa aaccttcaaa tataagttga tcgtacaatt gcgaatctga cattgatgcc
      241 caatcatcgt aactttccgc tttagccaaa gcttgttcgt tgtaatcgac atcaacatta
      301 gccagcgcat aatcgattgc atcacttgga tattcttcaa actctagttg ttcacgcaaa
      361 ccttgttcag acataccagc ccaacctaga tagctttcag ctttgctcaa tgcatttctg
      421 tattcacgag gaacactttc ttcttgagta acttcttcta tagtagtttc agtagtttca
      481 gtttcttctt caacgactgt agattctgct accgattctg cagaagacac ttctgtcacg
      541 ctttctttag ttgtttcagc gacggtattt ttagcatcat cactgcctcc ttgcgaacct
      601 atagcaataa acaccacaac aaccgctaac aaccaaaacc atacccgttt ataaaacggt
      661 ttcttaactt tatacatttt cccatcttga cccataactt tttttgccat ttaaatatcc
      721 ctcgcttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc cgtgttgcag
      781 cacgggcttt tttctttata acttttttag agattatagg caaaatagta gggcataaaa
      841 atgtattatt gaattccgta tttagaaaat cctaattgaa cttcaccgga agtcttttgc
      901 tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca tgcaacaggc
      961 gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc ttgctgagcg
     1021 cttgtttgtt ggtcttctgg attctgttga gatgctgctt gttgttcttt ctgatcttga
     1081 ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc cacgccgaaa
     1141 gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg taaagtgcgt
     1201 tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtact ctgactggag
     1261 cttataactg ttgggctagg ttccgccgta gcttggttgg agcttgtaac tgttgaacta
     1321 gaatctgttt tagatgtaga tttgctagta gatgaactgg tttcagttgt ttcttttgtt
     1381 ttacttattt ttgtttcttg gttagaagtg gtatctgttg attcagcttt tttattattt
     1441 gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac ttttttcatt
     1501 ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt atataagaaa
     1561 acgataagcg ctttctggaa gcccgtaaag attctttaat tcctcgattt ttttaggata
     1621 ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc aattagcttc
     1681 gctttcagat ttgcttctag atgttcttgt tgatacataa tagctggata agccacgatg
     1741 aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctcgcag
     1801 ttcatcattt aaaaagatta tcgcacgatc tctaatttct tgaaactgcc ccttgggatt
     1861 ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt
     1921 agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag
     1981 ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaaa gtcgctatca
     2041 ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt ttccccacca taagacatat
     2101 taacatttga gtctagtagt ttatcaagtt caaccacttc ctcttttgta gcccacttgg
     2161 gaacattatt atttcccaat aaataatcag ttgtgacacc aaaataatca gcaactttct
     2221 ttaagttctc agattttggc gaggctttat cccatcttct tatttgtcca ttagagatgc
     2281 ccacctgtct ttctacttct gctatagtca catgcttttc gtctgctaat tctttaatct
     2341 tagtaactaa actcattatt atcaaccttt caaagctgaa aagaaaataa atagcttaaa
     2401 agttatattt ttggttgaca attagctttt aagctagtat atttaattcg taagctaaat
     2461 tgttagctaa ataagagcaa caaaaaactc tactaattta aaacattctc tcggtcgcca
     2521 aacttagaaa tgttatttta gaggcttttt ataagtctta tttaactatg tattcataat
     2581 agcttaaaag ctaataggtg tcaacgattt agctaatttt ttagcttaca aattatttgt
     2641 ttagaaagga gctattttta tgtctgagaa tttagactta aaaattcgag cggagatgag
     2701 aaaaagaaga atgactttca aagaactagc tgcgcttatc ggtatttcag gagcttattt
     2761 atcagatatt ctaaacggca atcgtgatgg aaagaaagca caacagcata tcgaaacagt
     2821 gaaaaaaata ttggacatcc gatagggggg gtaggcgatg acaaaactaa aaaaacaaga
     2881 ttttgtaaaa aaatataatt attctccatc tacttatcaa cgtcgaatgt cggaactaaa
     2941 aaatacagca attttctcag cggcgtatga acgggtcaca ggacaagaag tttggatcaa
     3001 tacagaatta tacgataaat ttttgtcttt caaatcctat aacaggttac gcacaagaaa
     3061 ggtaacacct aaagaattta tcgagaagca tttagttgat ttataaaaaa cagaacattt
     3121 tgagaggtga aggttaatgg gtaaattcaa cagagcatta gtattcagcg caccgctaat
     3181 cgtctatgct ttagggcttt ggggaagtag acaagcgttg ataggaacga ttgtgtacat
     3241 ggtttggatt tttatagggc tggatgaagc tgagtacaaa acaaaaaagc cagtcgggag
     3301 ggactgacta atgagtaagg acgatattgt atttctgtta attatgtttt gtgggattgg
     3361 atcaataata attaatttgt atttgattat taaagaaaaa tttttcaatt aaaatgagtc
     3421 ttttagcttt aacttaaatt ttttttgata attagctgtt cttatgacca ttacaagttt
     3481 ttcaccaggt aatatatctt tcatatctaa aacttctgaa aaagcgactg agtagccagg
     3541 agagattgct tgtgccaacg gtataggaga taaaaggcta cttttatgaa tgcttcccat
     3601 atcaaaacca ttggcgtgat aatgtgaaca attaattttc ttaccgttgt aaagatacgt
     3661 acattcgata attgagtttg gcatttttga tgggtttgca attacgccag taagaatcat
     3721 tcgattactt ctatcatgct taactaaaaa ataggagagc gtaatttttc ttttatcttt
     3781 aaataagtta tagagggata aaaaaaatcc tgaaatagaa atgattaaag taatagcgac
     3841 ttggtaatcg ctcaaaattt ttatcaagta attcaccacc tttttactca aattatatca
     3901 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaaa aagacacgaa
     3961 cgccggcaag catgatcgtg tcagggaaat aaactatctt tctatatttt accacagaaa
     4021 ggaatgaacc gtaaatgcta attgcaacgg atacactaga ccgcatcttt ttaaaagacg
     4081 aatacaaact gcgcaatata gatgcgtcag gaattttagt atttgatctt tatgacaatg
     4141 gaaaaattgg tatctatcaa gcaagtgata ttgaagaaac aaaccttgct ttcgaacaaa
     4201 ttgatgattc tgtggaattg gatttagatg aggcaatcct agcttttgaa caaattgcaa
     4261 aattattaaa ggaggcacaa aagaatggca actctttacc aactcagcga gtcatatatc
     4321 aaagtcctgg aactggcaga acaattggat gaagaaattc ttcgcgatac tctcgattcg
     4381 attaatgaag cgatcgagta taaggcagaa aacttagcaa aaatagttaa agaagtagaa
     4441 gggaaagctg agttaatcga tagtgaaatt aaacgtctgc aggaacgtaa gacatcgctt
     4501 ttgaacaatg ctaagagtat caagcactat ttacaagagg aaatggaaaa gactggtaaa
     4561 acgaagatta aaggtgaatt attcaacatt ggaattcaaa ataatccagt atcggtcaat
     4621 gtaatcaatg agaacttaat tccaaaagga ttctttaccc ctgtgcctcc caaattggat
     4681 aaaaagcaat tgaaggagga actgaagcac ggagatattc ctggtgctga actcgtccaa
     4741 acaaaaggtt tgaggataag atgatgaagg aggagaaata atggaacacg aaaacgtaaa
     4801 agacgctttg aaagctgcta tcgaaatcgc ggaagctaaa ggaataaagg ttgacggcaa
     4861 accagcaact gtccatgaca ttcaaaattt gacgaaagaa catctttatt ttatcgccga
     4921 cttgcttggt ttgtcggaat tgtatttaga caaataaccc cacacggaag gagtggctag
     4981 ggtggttgta ttacaagtaa tagtctgcgt aacattcatt tggtcgtgca taccggtttg
     5041 tatatatatc atgaagaagc ttcgccctga gtcttacaaa ttatacgcaa tttacagcat
     5101 aacagtagct cttgcattta cattgtttgc aatttctagg cttccgtttt aatcgtggga
     5161 atgaattttt aatgaactaa attatctata aagcaaaaaa aggagaaaca acattgaaaa
     5221 acaaactagc aaaaacaaca gcaatcatcg gactagcact aggtagcggc gttatcggct
     5281 acgcagcaag caatgcgttt caagatttgg acacgatcaa agcaaatttc aacacagtcc
     5341 tacaatacgg tcaaacgaaa tcgcaacgtg tgtcagaact tgaatcacag ctatccaaca
     5401 acactcgcac acaggagcag ctgaaagccg aaattgagca aatcaaatcg gacaagcaga
     5461 aggaaattga agccaagcaa cgagaagtcg aacaaaagca acaggagatc gctacaaagc
     5521 aacaggaagc cgatagcttg cgccaacaac tatccacagt gcaaaacgac aaggagcagt
     5581 tagaacagcg cgtgagcgag ttacggcagt atacggatca aaaggtaggg gagttgggga
     5641 aatgaaagag ttgactattt atgacccaaa tatttcatgg gctaaacaca caattaaagt
     5701 ttcttttatg atttggggtt acaaaggata tgttacttac aaagtgggcg gtaacactaa
     5761 gggtttatca ttaatagcta ttgattcaga tgatctatat gatgctaatt tcgaggataa
     5821 cccagttaat ttccgtgacc ttgatgaaga ttggttctct atggaactta ctaacgataa
     5881 aggcgacagt acacttgtag aagatgaatt cgacagacta ggtgattata ttgtcggtgt
     5941 tgaaattatt gcgcacgaac cagagtagga gagtgaatac aaaatgatta aaagaatcac
     6001 tatatcagtt gaagaagata gtggatcaac aatgcagcat accgacaatc gaaaaacttt
     6061 tttaaccaat agaaaggaga attttgatgc agattaagaa ggcgacggat atcgaacaaa
     6121 ccaagggaac atatttgatc tacggtgctc caggaaaagg taaaacttca acggctaaat
     6181 attttccagg gaaaacactg atccttgata ttgatcggac ttcaaaagtc cttagaggtg
     6241 agaaaaatgt cgatatcgta tatatcgaca acgaagatac ctggaacgac tggggcaaca
     6301 cgttggctga tttaactacc aattatgtag gtgtttatga caacgtggtt gtcgataatg
     6361 tcagcgaatt ggaacggtgt attctgtcca gtttaggcgc agaaggaaaa aacaacggcg
     6421 ttccttcgca aggcgattat caatatatgc aatttagaat ggtgaacagc ctacgctata
     6481 tgaaaaattt agacagcaac cttatctgga ccgcatggga agaaatcgat ttgtggacgg
     6541 attcggatgg atcttcttac aacatcgctt tgccgcagat caatcgaaag attcgaaaca
     6601 atattttggg attatgtgac gttgttggtc gattgatggt aaaggaagac gatgaacgcg
     6661 gatttatttt acaagccaca gattcaacct atgccaaaaa tcaattggat aatagatctg
     6721 gctgtaagca aagtgagctg attttagatg gaattgtacg actaccagaa tgatttagtc
     6781 gatcgtgcaa gacaagccta tgttgatggt tacaaggcac catgtatcgt atcaccgtgc
     6841 ggttctggaa aatcagtcat ggttgcagag atcgctaaac gaaccacacg aaaaggcaac
     6901 agagtgttgt tcttggtcca tcgtcgtgaa ttgattgatc agatcaaaga tacgtttcaa
     6961 aagatgggcg tagataatca gcttgtaaat ttcggcatgg ttcaaacagt cgtacgacat
     7021 cttaaaacca tcaagaaacc acaactgatc atcactgacg aaaaccatca cgggttagca
     7081 gcgtcctaca gaaaaatcta tgagtattat tcggacgttc cgcgattagg ttttaccgcg
     7141 acacccatcc ggttaaacgg tagtgggttg ggggatgtga acgacttgct aattgaagga
     7201 gtatcggcta aatggttgat tgagaatcat agattagctc cttatgaata ttatgcacct
     7261 aaattgatcg atacagccga attaaagaaa gcttcgactg gagattttac aaagaaatca
     7321 atggataagg cagtgaagaa tactatttat ggcgacgtgt tgaaacatta taaaacgtta
     7381 gcagaaggtg aacaggcaat agcatactgt cacagtatcg atgcaagtaa acacactgct
     7441 gaaatattca acgatgctgg ttataaagca acacatatcg atgcaaaaac tccaaaagat
     7501 gaacgtgcag atattatcga agcgttcaga aatcatgaaa ttaaaatcct atgcaatgtt
     7561 gatttgatcg gagaagggtt tgatgttcct gattgttcga ctgtaattat gttgagacct
     7621 actcaatcgt tatcattgta cattcagcaa tcgatgaggg ggatgcgtta ccgaccagat
     7681 aaaacgtcaa tcatcattga ccatgtgggt aatgtgaacc gttttggatt gcctgatatg
     7741 gatcgtgaat ggagtttaga tacaaaaaag aaaacaaaaa ctgatagtga tttatctatc
     7801 gttcaatgcg cgttttgttt tggtgcttac gaacggccaa aaggcgataa tatttgtccg
     7861 tattgcggag agttgcagcc gattgaagaa cgaaagtcag agttagaaat cgatgaatcg
     7921 gctgaattaa tgaaagtcgg agaaacgaaa attactttga attttgagaa caataaatat
     7981 tacaacatga cggaagatga agcagaaagt atagaggact tatatgccat agcaagagct
     8041 aaaggattta aacctggttg ggcttatatg gctgctaaga ggaaggggtg gttgtaggaa
     8101 tgggcggtat ttttggtaaa aaagcagaag atgttcaagg ttcaataata aatggcgtaa
     8161 caataatagg tgatactgga aaaagagata gttcaggcaa tcaaattgta atcgctagaa
     8221 ataaaaataa cgaagttttt gaatcttctc tatctaatat aaaaagaggt gttgttaacg
     8281 ggacaggcga agagagtggc tacgttaatt taaaaaaagc taatgaaaaa taccatgttg
     8341 aaggaactta cgttaaggct ttgtctgtcg gtggaacaaa aggcgttaat tgggataaac
     8401 gaagaaataa atggaaagca cgattatatt tcaaaggaga atatgtctta caaaaggatt
     8461 tcaacaccaa agaagaagca atccaagcac gacttgcagc agaagaaaaa tatttcaaac
     8521 ctatcttaga aaaatatgaa aataaaaagg agaatgatta acatgacagg atttaactta
     8581 gacttttcaa aagcgcaaca agggaacgaa atcaaagacg gtacgtacga agtggtcgta
     8641 aacaaagctg tagaaaatgc aactaaatca ggcgcagaat ttatcgatat cgatttgatc
     8701 gttcgtaacg atgtggatca accgttccaa aataaacaca ttttcgcaaa aatctggaaa
     8761 gccaaagcta ccggcaaata taacgaaggt atgatcatgg caattgcaca agcgctacag
     8821 ttggaagacg gaaaatcata taacgggttt gatgaattgc tggcggactt tgttttgaaa
     8881 accgcctcag tacgcgtgaa aactgaagag tcaaatggat ataaaaacgt caatgtgaag
     8941 tcttgggata aaacgaacac acggggcgta atgaatcacc aattcaaaaa tggtgatgaa
     9001 ccatccttcg gaccggaacg ttcaagagcg acaacggtta gaaatgacaa tcttccattt
     9061 taattgaggt gtaatcaatg tatgaaaaaa ttccaaaaga gcttaaaaat ctcaaacaat
     9121 ggtgtgttta taagctagtt tgggatgaaa agcggcagaa gcacacgaag atcccttata
     9181 acgccaacaa cgggcataaa gccaaatcca atgacgagag tacctggtct gattttcaga
     9241 ccgctctcgc tgctattgat cactacggaa tgagtggact ggggttcttt ttcaaaccac
     9301 cttatttcgg cattgatatc gataatgctg aaggtgaagt tgagcgattt aaatcaggtg
     9361 atatagaaga aaatattatc tatgaattta tcgaaagcat gagatcctat gctgagtatt
     9421 ctcaatcagg tactggcatc cacattattg ctcgcggaga attgcccggc ggaagacgca
     9481 ggaaaggtga cgtagaaatg taccagaacg gccgattctt cgttatgact ggaaatgccg
     9541 catcaaaata tttagaaata actgagccga atccaaagga tatcaaacgc ctatatgatc
     9601 gctacgttgg agatgaaaaa atcatccaat tcaaagaaga aaatccgctg atgaataccg
     9661 ttgatttacc ggttgaagaa attatcaaac gtgctgaatc gtcttcccaa ggggcaagat
     9721 tcaaagtctt tatgaacggc ggttgggaag ctttttattc ctcgcaatcc gaagcggata
     9781 tggcgttcgc caacgacttg gctttttgga ccggtagaga ctttgaaaaa atggatgaga
     9841 ttttcagagc gtctgcaatg atccgtagca aatatgacca aaaaagggga tcggcaactt
     9901 acggagaatc tctactaaat aaggcaattg ctgatacaaa ttccgtctac aaccccaaac
     9961 ggaaatctga ttttaagatt tttatcaaag accaggaaca gccaaaagag gaaaaatact
    10021 attcgtacga cgacaccggc aacgcggatc ggtttactga tatctacgga acgttagtca
    10081 aatattcgta cattgataaa tcttggtatt actacgatgg gaaagtttgg ctacaggaca
    10141 ataccggcga agttcggaaa atgatcgata cgacagtaga catcatggga aaagaaccgt
    10201 taacgatccc tgaaggcgca gacgatgaaa ccaaagaagc tttgatgaaa gcgaaagaaa
    10261 aacacgtcaa gcgttctcgc agcaatgccg gaaaaaacgc tatgatggac gaattgaaac
    10321 atcgactatc cgtattgccg gaagagttcg acaaagataa aacgctgttc aacacacaaa
    10381 gcggctatct gtctttgcat gacggtcttc tacacgagca tgagatagac aaaatgttta
    10441 cacgtgtttc aaatgtcgag tacacagaat cggtcgattg tcctatgtgg gaagaattta
    10501 ttaatcagat ttttgacaat gatcaagaat taatcagata tatccagaaa tgtgtcggat
    10561 attccctgac cggatctacc agagagcaat gtatgttcat cctctacggt catgggtcaa
    10621 atggaaagtc ggtatttttg gaaattattt ctgaattgat gggaaactat gcgatgacta
    10681 tgcaggcaca aacgatcatg gtcaagcaaa gccaatcatc agcaaacagt gatattgcac
    10741 gattgaaagg tgcgcggttg gtcacttcgt cagaaccgaa tgaaggcgtc cgactggacg
    10801 aaggtctggt caaacaattg actggtggtg ataaagtcac ggctcggcac ctttatggaa
    10861 aagaattcga gttcgaacct gaattcaagt tgtggctggc aacgaaccac aagcccatca
    10921 tccgtgggac cgatgacggc atctggcgac ggttaaattt aattccattc actgtccaaa
    10981 tcccggatca caaaaaggat aaaaatctga aatttaaatt gcagacggag ttgcaaggga
    11041 tccttaaatg ggcaatcgat ggttgcctgt tgtggcagag agagggcttg gagaaaccaa
    11101 agtcggttgt tgcagcgagt caggattatc gaaacgaaat ggatcaaata ggtacattta
    11161 tcgaaacgtg ttgcgaaaca ggtccaggac taaaaatatc aggcggagaa ttatacaaag
    11221 tttatcgtga atgggcatca gataatggcg agcatacttt cactaacacg aagtttggca
    11281 gagagatttc aaagaaatat agcaaagaaa aagctggtgg atttatggtt tataaaggaa
    11341 tcacattaaa accaagaaaa tacgataacg ttagagaact ttttaaataa atctggaggg
    11401 ttctggaggg ttgagaacga aaccctccaa cccttagaaa cgttgatata tcagtattta
    11461 tattatattt ttctttcttt ctggagggtt ttaaaaaaat aataaagtat taaagtaaaa
    11521 aataaaaagt aaattatata aaaaagaata ggtttttggg tctgaccctc caaactctcc
    11581 accaaaatct tcctaaatgc tttgatacca acgattaaac agtctggagg gttacattga
    11641 aatcagaaca tcaaatacaa gacgagatca gaattgcctt atcaaaacat ggttgcacga
    11701 tttttcgatc caatgcggga caagtgacaa cgatcgaagg gcggcaattt tacgggatgc
    11761 caaaagggtt cccagatcta tgtggacatc gtaattctga tgggaaagca atttatatag
    11821 aagtaaaaaa tgaacgcggt aagttgcgtg aagatcaaaa aaggtttgct gaatttttaa
    11881 acggtcaacc tgtactatac ggtgttgcac gatcagtgga agaagcattg aaaattgtgg
    11941 aggaatcatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga
    12001 gattgacgat ctggaacgga caaaggcagt gatggtcaat gaaacggcga ggaaggcaat
    12061 cgatttgcac atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat
    12121 aagaaagcag caatgaaacg aatcatcgaa ctgacacatt ctgagaattg gcaagaagac
    12181 aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggagtga aaaaaccaaa
    12241 cggagaacac cgagaaaaat tgcaatctgg catggtgacc gaattctagt aacaggtact
    12301 gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctaaa
    12361 cgcgagaacg ttgattcgaa aggtcgtcaa tttaaacatt gggagaagaa ataatggagg
    12421 aactaatcac aaaagtagag cagtgggcaa aagataaggg attggatcaa gctgattcca
    12481 gcaagcaaat gttaaaaacg atcgaagaga ttggggaagt tgccgcttct ctagctagaa
    12541 aagatgaata tggtttaaga gacggaattg gagacgtagt agtaaccttg attattttag
    12601 ctatgcaaaa tgatatggat ttgtacgagt gtctgaacca agcatacaac gaaatcaaag
    12661 gacgcacagg aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gaggacagcg
    12721 atgaataaaa aagtattaat tgataaacaa gcattgattg atgaattaat gaaaatacct
    12781 ggtgtgggat ctaatagtga cgctttagaa acgattaaac gtttcccatc gtatgaaccg
    12841 cagaagccag ttgtgcctaa gtttgtggcg gaactactcg actattatcg tcagtcaaca
    12901 gatgttgatt tattagcttt attgataact ttccatgatt ggtattatcg caaaactaaa
    12961 gacggtgagc atgaagaagc aattgattgg ctagttgatc atcctgaaat ttatatgcgt
    13021 gcatggctgt ttggctacga gctcgagaaa gagccgttgt attgcgtaat aatcaatggt
    13081 agatatcttg tcaaagtatt cagcaataca aacgttgtca tattagttcc agcagatgaa
    13141 ttttcacagt acgttactca aaaatttcaa ttaactgaaa aacaaatcaa agcaattgat
    13201 gaaagatact ggccgtttgc tgtgccagtg gatgaggtgg tagaaggatg agcgacccat
    13261 acattactaa attagaaaag ttgtccgaag attttgagaa gaataaaaat aaactaacta
    13321 aagataaact tatagaactg gttaggttgg ttatcagagg agctattaca agagaaaagg
    13381 cgcagcaaaa catagaaatt tttgctcgta gtgtccgaga tggattagag aagaaagtta
    13441 aacaaaatga acaacagaca ccgcagaata acaaaactaa gaaaacggga actgaatgta
    13501 ctaaagacaa agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca
    13561 agtcagtgtg ttgctgatgc gagcggcgct attcgtaagt ttggaatttc gatattaagt
    13621 gatgatcgta aatgggagga aaaagaatga aactaaaaga cggattttac tccagcagtc
    13681 atggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtgagaaac
    13741 caaaattcaa agtcggtgac atggttcgct gtgaagcaga aggattcatc tatccatttt
    13801 gtggatatgt agaacatctc tataatcact cagcaatcat tcgtattgaa aacacgatgg
    13861 aatgtgacaa gtggttagcg aaaagcaaag agaatttagc agtagcgaga ttggtggata
    13921 tggaggttat ataaacagcg aaaaaatgta cttctaagag aaaaatttat tttttataaa
    13981 aataggaaag tatcattatg aaaaagacca cagagataaa taaacctacc acataaaaaa
    14041 tgaatatttc ttgcgattca atataggtat atttaacaat ttgtacaata aaaaatacta
    14101 taggtaaaaa agataacatg aggagcttat ttgttctaga gtgtttgaca aagtaaaaaa
    14161 atgcgacgat accaatacaa acgggaatcc aaaataacag catgctccac atagtcactc
    14221 aaccatctcc tatacgcttt attatcaata gataatatca tttttcgtaa taaagataaa
    14281 aaaataagta aggcttctca ttaaaatata aaaaagacag ccgaccactg actgcctata
    14341 taagagtatt gaaataaaaa gctgctgata taataaattc cacaagttta ttatatcaca
    14401 taaaggagcg gtttgacttg atgcaattgt tacgagaggt agatttcaaa cagacaagat
    14461 gtaatgcgag agatgtgctg aagaactttc ggcgtttgga gcggatggca ggtcgctctt
    14521 tgatagatat taagtcgccg attattacgg atatgccgaa ggcgccgaag cacggcaata
    14581 aggcagaaga cgcaatcatt cagatgatgg atatagaagc ggagagagac gcgattctag
    14641 cagccttgat ggctcttagt ctgattagtc gtcagatact ctactatagc ttttgtgtgc
    14701 cagatagctt ctcaaactac agaattagcc gtgaagtggg ttattcagaa agaagtatac
    14761 aacggatgaa gtcggaagct ctaatagagt ttgcagaagc atataaacac ggaagaataa
    14821 ttgcttataa ataatttggc ggctttttgg cggaatgatg gcggttttta gctatttacc
    14881 agtgatatta tggtagtgtc gaaagattag tgataggtct
//
LOCUS       DACDEA010000062        14489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDEA010000062 DACDEA010000000
VERSION     DACDEA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.062"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            384..716
                     /locus_tag="IV136_002052"
     CDS             384..716
                     /locus_tag="IV136_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8466026.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1012..1167
                     /locus_tag="IV136_002053"
     CDS             1012..1167
                     /locus_tag="IV136_002053"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8466027.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1443..2783
                     /locus_tag="IV136_002054"
                     /pseudo
     CDS             1443..2783
                     /locus_tag="IV136_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3024..4640)
                     /locus_tag="IV136_002055"
     CDS             complement(3024..4640)
                     /locus_tag="IV136_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8466028.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4758..4976
                     /locus_tag="IV136_002056"
     CDS             4758..4976
                     /locus_tag="IV136_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466029.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5171..5632
                     /locus_tag="IV136_002057"
     CDS             5171..5632
                     /locus_tag="IV136_002057"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466030.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5825..6064)
                     /locus_tag="IV136_002058"
     CDS             complement(5825..6064)
                     /locus_tag="IV136_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466031.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6088..7611)
                     /locus_tag="IV136_002059"
     CDS             complement(6088..7611)
                     /locus_tag="IV136_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8466032.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7629..7751)
                     /locus_tag="IV136_002060"
     CDS             complement(7629..7751)
                     /locus_tag="IV136_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8466033.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7781..8764)
                     /locus_tag="IV136_002061"
     CDS             complement(7781..8764)
                     /locus_tag="IV136_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8466034.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8788..8937)
                     /gene="rpmG"
                     /locus_tag="IV136_002062"
     CDS             complement(8788..8937)
                     /gene="rpmG"
                     /locus_tag="IV136_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8466035.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8958..9335)
                     /locus_tag="IV136_002063"
     CDS             complement(8958..9335)
                     /locus_tag="IV136_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466036.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9367..9546)
                     /gene="rpmF"
                     /locus_tag="IV136_002064"
     CDS             complement(9367..9546)
                     /gene="rpmF"
                     /locus_tag="IV136_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8466037.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9561..9830)
                     /gene="rpsN"
                     /locus_tag="IV136_002065"
     CDS             complement(9561..9830)
                     /gene="rpsN"
                     /locus_tag="IV136_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8466038.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10353..12125)
                     /locus_tag="IV136_002066"
     CDS             complement(10353..12125)
                     /locus_tag="IV136_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466039.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12079..13833)
                     /locus_tag="IV136_002067"
     CDS             complement(12079..13833)
                     /locus_tag="IV136_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466040.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13943..>14489)
                     /locus_tag="IV136_002068"
     CDS             complement(13943..>14489)
                     /locus_tag="IV136_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8466041.1"
                     /translation="IGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVY
                     ISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQ
                     VASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLV
                     VASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4628 a         2822 c         2215 g         4824 t
ORIGIN      
        1 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
       61 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
      121 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
      181 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
      241 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
      301 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
      361 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
      421 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
      481 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
      541 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
      601 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
      661 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
      721 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
      781 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
      841 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
      901 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
      961 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     1021 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     1081 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     1141 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     1201 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     1261 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     1321 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     1381 acagacacct taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca
     1441 agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct
     1501 ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg
     1561 gcacattgac ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga
     1621 tcattaaata gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga
     1681 ccattctgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag
     1741 cagaagattc agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc
     1801 ttgagctatt aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc
     1861 cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc
     1921 tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca
     1981 tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc
     2041 aactcaatcc tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc
     2101 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
     2161 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
     2221 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
     2281 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
     2341 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
     2401 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
     2461 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
     2521 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
     2581 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
     2641 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
     2701 gaaaatattt taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac
     2761 ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc
     2821 accaacactc attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat
     2881 tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag
     2941 cgcttaacac acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa
     3001 aaacctcctc atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca
     3061 atactaataa ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat
     3121 ccagttctaa ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct
     3181 ttaatctcaa caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg
     3241 atcttatcca aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg
     3301 atagcatctt cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa
     3361 gaggtaataa tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt
     3421 aaaaatttca ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa
     3481 atttttcgga aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta
     3541 atacattgtt gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac
     3601 aacggatgcc aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt
     3661 ttaggtaaac tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt
     3721 tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta
     3781 atattgccag gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac
     3841 cgatttcgat agataaaact tgagatacca tattcttctg tttcaggaat atgattttga
     3901 tagccaagct ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa
     3961 agatttgtat ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg
     4021 gcattggact gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact
     4081 aagtcactat tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca
     4141 cgaatatcct gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca
     4201 cccgtttcga aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc
     4261 tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata
     4321 attggaattg gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat
     4381 tgcactaatt gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca
     4441 ttcggcatcg aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc
     4501 ccatcaacat ttacttcccc tatgtcattt acaatgacag caatttcctt tccgtgatca
     4561 ccgtacagaa tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt
     4621 gtaataggta tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg
     4681 caacacataa aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc
     4741 ctagaaggaa aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa
     4801 tctttctgct tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata
     4861 gcccgtttac tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa
     4921 agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga
     4981 tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga
     5041 aatctcgaat cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta
     5101 acgtgcaatt tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct
     5161 gatccgttca ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg
     5221 tggaaagatt tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag
     5281 tgattttggt cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt
     5341 gactttgaca gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa
     5401 agccgcttat gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt
     5461 caaaacgatc actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc
     5521 attgaataat atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat
     5581 taaaatacga atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta
     5641 gataactcaa cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta
     5701 gattggagaa caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc
     5761 aagacataat aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta
     5821 tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga
     5881 ccttgataaa acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa
     5941 catgtaaagt tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa
     6001 atcgataacg ccgataagca tcaatattct tggaatccat agacaataat acttttctta
     6061 ccatgtattt cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg
     6121 tttagacaat gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc
     6181 ttgactttct ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg
     6241 atcactaaat tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa
     6301 acgaacacca cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt
     6361 gtattgatga tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt
     6421 atcaatatct gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc
     6481 tttattgatt tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg
     6541 ataaacagat tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc
     6601 tttaaagtac ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc
     6661 ttcaccagca accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac
     6721 ataatcttca ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc
     6781 caattttatc ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc
     6841 aaagtaaata taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc
     6901 agaagatggc tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc
     6961 caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt
     7021 aagactagtg gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt
     7081 tgtctcaaat acttttgctt ttttcggata caatttaact aactgatcct tgatatttga
     7141 aacttcctta attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg
     7201 atcttcttct ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt
     7261 tgttccttct actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca
     7321 tgattccatg ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc
     7381 cttagctgag ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt
     7441 cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat
     7501 ttctaatttt ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc
     7561 caataatcct agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc
     7621 ttccttaatt atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat
     7681 gccctttttc tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc
     7741 gttttctcca ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta
     7801 atttatcaaa actctgtttg atcctttctt catccaacat ttttccgatt agaataatag
     7861 tggtttggtc tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca
     7921 tggcaactat aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc
     7981 cttggttggt taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt
     8041 gatctgaagt taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt
     8101 tgccattata gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt
     8161 tcaaattata aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg
     8221 ataaagggtt aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt
     8281 tttgttttct agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt
     8341 ctctttcaat aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata
     8401 ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta
     8461 tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca
     8521 gtaaagaaat caatattgct aataaatcac tgcgaagact gcaacacata catccattat
     8581 ttagttgtaa cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac
     8641 caaactcatt ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt
     8701 gattgattag cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc
     8761 ccattttgtc acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg
     8821 caattttggc gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct
     8881 ggtgagatac agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa
     8941 tcttcctcct tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca
     9001 aaagttaact cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc
     9061 tcccaataac catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag
     9121 attcttaaaa aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca
     9181 ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc
     9241 gaacgataaa aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat
     9301 ccttcttcaa ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat
     9361 attaaactat tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga
     9421 tacatgatgg ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac
     9481 tacagtttga tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac
     9541 tgccatgata tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga
     9601 tgggcaagtt ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga
     9661 cgaccatccg tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta
     9721 gcgagagcgg tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca
     9781 atcatcgcca tttgtttctt tgctttagca attttcgact tttttgccat atacctttat
     9841 actcctttta ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac
     9901 tttaccaaca agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat
     9961 agataactat ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa
    10021 tatgcagttt aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat
    10081 accgttttaa gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt
    10141 tattcaatct tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct
    10201 tatattataa gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt
    10261 caaaattaga tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc
    10321 aaaaattgta ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag
    10381 acgaatttct cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga
    10441 tacaactcat tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta
    10501 tttacctttt caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt
    10561 aaacgattta aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt
    10621 tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt
    10681 cgctctcctc cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt
    10741 agtttttcaa taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct
    10801 gacgcttgac tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc
    10861 tcttgaaaaa caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca
    10921 tacccatcaa ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag
    10981 atactagttt tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc
    11041 gtaaaagaga tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc
    11101 aactcaatgt caaaagattt cagaggacta tctgatccct cttggatact ggtctccttc
    11161 atagctctaa ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc
    11221 atagaatcga aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt
    11281 atagtcactt cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta
    11341 acatatgaga aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa
    11401 tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga
    11461 ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct
    11521 ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt
    11581 aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc
    11641 aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca
    11701 tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat
    11761 aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact
    11821 cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac
    11881 aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt
    11941 gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta
    12001 atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca
    12061 cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt
    12121 ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt
    12181 tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct
    12241 ggataatcga tgagctatca taataactgt tttttctttt atcagatgtt gaaaagcctt
    12301 ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat
    12361 ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag
    12421 ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga
    12481 ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc
    12541 tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc
    12601 aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat
    12661 ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct
    12721 tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc
    12781 taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt
    12841 aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc
    12901 tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat
    12961 gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat
    13021 cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat
    13081 gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata
    13141 tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc
    13201 accaaatatc tttataactt gaattccgcg gatatattct accgtttcag agcttaacac
    13261 atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa
    13321 gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa
    13381 gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg
    13441 agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga
    13501 agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct
    13561 caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa
    13621 taaagctcct aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc
    13681 atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt
    13741 taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg
    13801 gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt
    13861 ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa
    13921 cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca
    13981 atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc catataccta
    14041 ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt
    14101 gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt
    14161 gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa
    14221 taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc
    14281 ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag
    14341 ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg
    14401 atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta
    14461 ctagggaaat aactgtagct aaaccaata
//
LOCUS       DACDEA010000063        13874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDEA010000063 DACDEA010000000
VERSION     DACDEA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.063"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..1133
                     /locus_tag="IV136_002069"
     CDS             141..1133
                     /locus_tag="IV136_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8466042.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1193..2614
                     /locus_tag="IV136_002070"
     CDS             1193..2614
                     /locus_tag="IV136_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8466043.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2607..4106
                     /locus_tag="IV136_002071"
     CDS             2607..4106
                     /locus_tag="IV136_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8466044.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4145..5539
                     /gene="uxaC"
                     /locus_tag="IV136_002072"
     CDS             4145..5539
                     /gene="uxaC"
                     /locus_tag="IV136_002072"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8466045.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5698..6381
                     /locus_tag="IV136_002073"
     CDS             5698..6381
                     /locus_tag="IV136_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8466046.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6383..7219
                     /locus_tag="IV136_002074"
     CDS             6383..7219
                     /locus_tag="IV136_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8466047.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7230..8984
                     /locus_tag="IV136_002075"
     CDS             7230..8984
                     /locus_tag="IV136_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466048.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9260..9601)
                     /locus_tag="IV136_002076"
     CDS             complement(9260..9601)
                     /locus_tag="IV136_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8466049.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9669..11840)
                     /locus_tag="IV136_002077"
     CDS             complement(9669..11840)
                     /locus_tag="IV136_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8466050.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12051..12908
                     /locus_tag="IV136_002078"
     CDS             12051..12908
                     /locus_tag="IV136_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8466051.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(12979..13779)
                     /locus_tag="IV136_002079"
     CDS             complement(12979..13779)
                     /locus_tag="IV136_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8466052.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(13776..>13874)
                     /locus_tag="IV136_002080"
     CDS             complement(13776..>13874)
                     /locus_tag="IV136_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466053.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         4218 a         2791 c         2692 g         4173 t
ORIGIN      
        1 aatagcataa ttaaaaataa aaatccaagt aatttttaac gtgtgcaatc ttttcatatc
       61 aaagatctat tgacttatcg tagttatcgt aatatgatga atgcgttaac aaaaagtgaa
      121 aaagtaagga aaatgatgga atggtaacga tcaaagatgt cgctaaagca gctggcgttt
      181 ctcatacgac agtttctcgt gctttgaata acagcagttt aataaaaaat gaaacaaaac
      241 aaaaaatacg acaactagct aaggaaatga attattcgcc aaactatagt gcaaaaggtt
      301 tagtgaatca aaaaaaatat ctaatcggac tatttttctc aagtttgcat aaaggaactt
      361 catcaaattt cctagcagat tccattgcag gcatccattc agtattagac acaaattaca
      421 gtttatctgt tgaaagtatc gatgttgttg atttaacaga aatcaacctg caacgctacg
      481 atggactgat cgttatgagt caaagtgatg atgatcagcc tttcatcgac tatctcaaag
      541 aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa tgatccagcg gttatcaacg
      601 tcactgccga tgatgcggcc ggtgttatgc aggcagtaga ttacggtatt ggatgcggac
      661 atacgaagat cgcttatatt ggcggaaaga aaaattttcg ttcgacaaaa gaacgggaaa
      721 aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt caatccagcc tattttttaa
      781 caggagatta cagcttagaa agcggcctcc aaaacatgga atacctatta tcactttctg
      841 acctgcctac tctggttttt tgcggcaacg acgacatagc gattggtgca ttgagagctg
      901 tacatttagc tggcctccgt gttccccaga acatttctct gatcgggttc gatgattctc
      961 cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg taagcctttg aaagaaatgt
     1021 gtcaagaagg aacaaagctt ttactagatt tgatcgaagg caggacgatc gaacaatcta
     1081 aaattgagct ctccaccttg ttgatccttc gtgagtcgat caattacttg taaactacaa
     1141 gtaattttat ataaaaatgt taacgtgtgc ataacacaga aaggaagttt atatgaaaaa
     1201 actgacaaaa gaattagtga aaaaaaacga agcacctgta aaagttcttc agtttggtga
     1261 aggcaatttt atgcgtggat ttattgattg gcagattcag cagctgaata accaacaatt
     1321 gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa ggtcttggaa atatgatgaa
     1381 ccagcaagat aatttatata cggtcatttt acaaggactc agcaatggcg aaatcgttga
     1441 tacttccgag attatcaccg tagtagaaca aatgatcaat ccttatgatg aatgggatgc
     1501 ttatctatct ctagcagaaa atgacgactt ggcctatatc gtctccaata caacagaggc
     1561 aggaatccaa ttcgtggaga ctgataattt aacagatact cccccacaga gcctcccggg
     1621 caaactgaca gcctttttat accgacgttt caaattaaac aaagctggct ttaccatcat
     1681 tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa gaaatcattt tacgctacgc
     1741 agaaaaatgg gatttagaac ctgcttttgt tgagtggtta gaaaaagaaa atgttttctg
     1801 ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc gaagatgcag gtgttttcaa
     1861 tcaacggcat aactatgaag atcaattgat ggtcaaagca gaaccattca tgctttgggt
     1921 gatcgaaggg cctaaagaat tggagaatca actaccattc aaacaggctg gcttgaatgt
     1981 catcatcaca gatgatatga ctccttatcg tgaacgtaag gtgcatttgc ttaatggacc
     2041 gcatacagcg atggtccctc ttgctttgtt ggcggaattg gaaactgtag aagaagtaat
     2101 gaaagatcct ttattctccg catatgttga tcagttattc aatctagaat tgattcctat
     2161 gctttcttta cctaaagatg aattagccat ttatgctgac caaatcaaag aacgtttctt
     2221 gaatcctttt gcccatcaca aattagaagc gatttcatta aatagtgttt caaagttcag
     2281 cacccgtctg cttccagtgt tcaagaagta tatcgaagaa caaaatcagg taccaccgtt
     2341 aatcactgtt tcactagctg cgctacttct tatgtatcga ggcgatcaag taaagccaca
     2401 tgatgatgaa aagacgataa gcgaatttac tgatgcttgg tctgataatg gaacagctat
     2461 tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat ttgtctcaaa taccgaatgt
     2521 gacagataca gttcaagaaa tagctgcaaa gatcgatcaa aaaggtgtcc ggtcagtatt
     2581 acaagaaatg aaaggaacga tggtccatgc ttgaaaccct gcaaccaaca atgaaattaa
     2641 atcgacgaga cagtgtggtc gtcgcattaa cccctattcc aaaacaaaca caactgacga
     2701 ttgatcagca aacgatcacc actcttgaag atatcccgca aggtcataag attgcattgg
     2761 gtgatttgaa agctggagac aacgtgatca aatatggcta tccaatcggt catgtgacga
     2821 cagacgtaac tgccggtcaa tggctgcaca ctcataatgt aaaaaccaat ctttctggtg
     2881 aattggatta tcgatatgaa aaagatgtcc atccaagtca ttaccttttt gagaaccgaa
     2941 catttcaagg ttacttgcga aaaaatggaa aagtagggat tcgtaatgat ttgtttttag
     3001 tacctaccgt tgggtgtgtg aatggcattg ctgaactgat cgtcaaacag ttcaaggaaa
     3061 aacatccaga tctgggacag tttgaccata ttacgatttt aaagcatcct tatggctgtt
     3121 cgcagcttgg aaaagatcat caaaatactc gagaaatttt agcggacgca gtaaatcatc
     3181 ccaatgccgg aggagtttta gtatttggat taggttgtga gaacaataca gttcctgaat
     3241 tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa atttctagtg gctcaagatg
     3301 tttacgatga gatcgaacaa ggtgtggctc tattagaaga gcttttaaca gcggcggaaa
     3361 atgaccagcg catatcagtc ccgctctcta agttgaatgt cggtttgaaa tgcggcggtt
     3421 ctgatggtct atcgggaata actgctaatc ctttacttgg tgcattttct gactacttga
     3481 ttgctcaagg aggcagcacg atcttaacag aagtgccaga aatgtttggc gcagaacaag
     3541 tattgatggc acgagcggaa aatgaagaag tttttgactc aatcgttgac ttgatcaatg
     3601 acttcaaaca gtatttcttg tcttacgggg aaccagttta cgaaaaccca tctcctggta
     3661 ataaagcagg tgggatcact acattagaag ataagtcttt aggctgcacg caaaaatcag
     3721 gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa aatcagaaaa ccagggctta
     3781 gtttattgca agctcccggg aatgatcttg tagctgcctc tgctcttgct tcatccgatt
     3841 gccagttggt cttgtttacc acaggaagag ggactccgtt tggctcatat gtcccaacgg
     3901 tcaaggtatc tacgaataca actctttttg aacgcaaagg tcattggatg gattttaatg
     3961 caggcgtctt attagaacaa ccaatggaag taatcttaga atcgtttgtt caaaaaatca
     4021 tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa tgacgttcga gaaattgcca
     4081 tttttaaaaa cggagtaaca ctttgatttt cataaaacag tataaaaaga aagaaggaag
     4141 atttatgttt ttatctgatg attttttatt aaaagacgat tgggcgaaaa agctttacca
     4201 ttcctacgca aaaaacatgc ccattattga ttatcactgt cacttagatc caaaagaaat
     4261 ttacgaaaac aacaatttca gcaatctgac agaagcttgg ttaggaggcg atcattacaa
     4321 atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa atcacaggaa atgcttctga
     4381 ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa attatcggaa atccgcttta
     4441 cagttggtca catcttgaat tgaagcgttt cttcggtatt gacttgttga taaatgaaga
     4501 aaatgcagag aaaatttgga cacaagcaaa tgaaattttg gcagctcctg attttcgccg
     4561 tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt acaacagatg atcccgcaga
     4621 cgacttgcac tatcatcagt tgcttgctca tgaagaacca gatttgaagg ttctaccttc
     4681 attccgtcca gataaagcac tcaatattga aaaagaagga tttaatgcat gggtcacact
     4741 actggaagaa gcagctgacc aaacgatcac cacctatcaa gaactgatag aagtcttagg
     4801 gcagcggata gaatacttcc atcaacatgg atgtcgtgtt tccgaccacg ctttagacat
     4861 cttacgctat aaagaagcgg atgaaacagt attagaagag atttttcaaa aagcacgagc
     4921 taacaaacta ttatcttcag atgaaatcga tgcttatcgt acagaaacac tgatacattt
     4981 gacccacttc tatcattctc ataattggac gatgcagctg cacatccatg cttatcgtaa
     5041 ctgtaataca caaatgttgg aaaaattagg gcccgatacc ggctatgatg gaatgaatga
     5101 tctttctttg actattcctc tgcaaaaatt attgaaccgt gcagaagaaa cggatcagtt
     5161 gccaaagacg atcctttatt cattgaatcc gaatgactat cctgcgattt tagctttgat
     5221 gggctgtttc caaaaagaac agaatggaaa actgcagtta ggttctggat ggtggtacaa
     5281 cgatactcga gctggtatgc gcgatcaatt gacacaattt gctgatggta gtgctttggg
     5341 gaattttgtc ggcatgctga cagattcaag aagtttcctg tcctacacta gacatgaata
     5401 tttccgtcgt gttctttgtg aatttttagg cgaaatggtc atgcgaggag aagcacctga
     5461 agatgaacaa cttttaggaa gtctagttca agcgattagt tatacaaatg ctaaaaatta
     5521 ctttggcttt tttgcctaat tgattaatag taacagtaac gaatcaaaaa caggatagtg
     5581 aaataacgga atccgtttat ccacgtttta gagaagaaga cggaccaaat ttgctccgtc
     5641 ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag agataacatg
     5701 cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa caagcgtccc
     5761 gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca gatcaataac
     5821 caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt agcccgtcta
     5881 gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa cgaccagaaa
     5941 atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct gcagatattt
     6001 tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt gacgcgacga
     6061 gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc aaaagcgatg
     6121 cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag atcacaagaa
     6181 ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct tatacctgat
     6241 acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc agaaggcgct
     6301 gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc tcttgctaat
     6361 tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg cccacgattt
     6421 agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact tgtcccatat
     6481 ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca tgacacttgg
     6541 gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt ccattttagg
     6601 atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat tggcaacttt
     6661 caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta cggaaaccgg
     6721 tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc agatcgccag
     6781 aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg ttgctgttga
     6841 agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa tcgatgaagt
     6901 agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata aagaaaatca
     6961 tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt atattacagc
     7021 gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac ctgttggcaa
     7081 gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc ctctaatgta
     7141 cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac acttgcagtt
     7201 tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa agttatcaaa
     7261 aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat tattggcgtt
     7321 ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta atgccgatcg
     7381 ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg caggccattt
     7441 atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg aattattttc
     7501 atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga gcgaccctta
     7561 tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct ggacgaatcc
     7621 agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa gtatttgtca
     7681 gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt tataaaagtc
     7741 ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt actctctttt
     7801 tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa gagacttctg
     7861 caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg ttagaaaaac
     7921 acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga ttccacttag
     7981 atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata tttcagctga
     8041 tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc ggcgatgtcg
     8101 tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg atcacgctga
     8161 ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg ttgattgctg
     8221 gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga aattttcgtt
     8281 ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat catttttctc
     8341 tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct ggaaaatcta
     8401 cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt tttctagatg
     8461 atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc gttgttcctc
     8521 aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc ttgaaaaacg
     8581 tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca ctgattgacc
     8641 agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg tctggtggcc
     8701 aaaagcaacg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc attttgctag
     8761 atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa gccctgaatt
     8821 acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg atcaaagagg
     8881 cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact tacgaaaaac
     8941 tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa ataggagcta
     9001 tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct gccaactcct
     9061 cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat tgttccttgt
     9121 aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa gagacagcct
     9181 atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt cacaccctct
     9241 gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc atgatgatac
     9301 ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag tcattgatta
     9361 cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct tcgtcgctga
     9421 actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg aagtctttga
     9481 acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc aacgctttga
     9541 attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag atgtttgaca
     9601 tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt tattcgtttt
     9661 caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc accaattccc
     9721 tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc ttgttcacca
     9781 tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc tgtgtttcca
     9841 acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac actaaatcct
     9901 gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt cccgacaaca
     9961 tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc catccatgtc
    10021 gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt tgtggcatcg
    10081 aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg attcgcagca
    10141 gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc ggctgtttcc
    10201 tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc tccagtcgaa
    10261 tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc aaatgcactg
    10321 taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag ggctaagcca
    10381 taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc atatcctttt
    10441 tgtaaaccga tctcatgcaa aagccataca gtaggcaaat tcaaactttg tgcaacagct
    10501 tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc gtaataactt
    10561 tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc tggtgtatat
    10621 acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc aaagctgaat
    10681 cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc tgtattcgga
    10741 tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc tgggaataat
    10801 gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa agctgtatag
    10861 attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt aactgcttca
    10921 tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt atcgtatgtg
    10981 tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc ttttgtgatt
    11041 tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc attttttggg
    11101 tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt agcagcctca
    11161 gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc ttggacaccc
    11221 cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc ttctttgctg
    11281 tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc aaacgtttgg
    11341 tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc tccgccccct
    11401 gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt gatatcaaac
    11461 ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg gatataggga
    11521 gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc agcttcttcg
    11581 tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt ttcgacattt
    11641 gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat caaaccaatc
    11701 agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca tcgatggaat
    11761 tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt tatcttttct
    11821 aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat cgtcttattt
    11881 taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg tttttttacg
    11941 ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt cttaaatatg
    12001 ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag gagtacactt gtgaaaatca
    12061 cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt gaaaaggaat
    12121 ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt tggatcaatc
    12181 aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca ttgttattcg
    12241 aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac atccgtgttt
    12301 tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag actcatccaa
    12361 atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc gacactaaac
    12421 agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc gttttatttg
    12481 caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa atgatctatc
    12541 ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc accaataaaa
    12601 ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat ggtcagagcg
    12661 ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc tattgtgttt
    12721 tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga catccgatcg
    12781 ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat gcattagaac
    12841 tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta cctggattat
    12901 gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga ctctgttctt
    12961 tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca aactaccgat
    13021 aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt gaggtgtttc
    13081 catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga gatgatcctg
    13141 attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg tatagttgat
    13201 cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt gcggcagatc
    13261 ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc cttctaaaca
    13321 atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt tcacccaaat
    13381 atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca cgacacctgc
    13441 accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg ctgctccttg
    13501 cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag gaatattgta
    13561 gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg gagttgccaa
    13621 aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg agccaatccc
    13681 gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg tttcttctgg
    13741 tgttgtggta gggatactta attttttctt aattgtcaaa ttaagttaga cagatcatca
    13801 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
    13861 atattatttc gttt
//
LOCUS       DACDEA010000064        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDEA010000064 DACDEA010000000
VERSION     DACDEA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.064"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV136_002081"
     CDS             complement(<1..914)
                     /locus_tag="IV136_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8466054.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV136_002082"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV136_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8466055.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IV136_002083"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IV136_002083"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8466056.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IV136_002084"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IV136_002084"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8466057.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IV136_002085"
     CDS             4440..5321
                     /locus_tag="IV136_002085"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8466058.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IV136_002086"
     CDS             5318..6034
                     /locus_tag="IV136_002086"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8466059.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IV136_002087"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IV136_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8466060.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IV136_002088"
     CDS             6751..7752
                     /locus_tag="IV136_002088"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8466061.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IV136_002089"
     CDS             7749..9110
                     /locus_tag="IV136_002089"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8466062.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IV136_002090"
     CDS             9070..10221
                     /locus_tag="IV136_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8466063.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IV136_002091"
     CDS             10196..11365
                     /locus_tag="IV136_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8466064.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IV136_002092"
     CDS             11540..12919
                     /locus_tag="IV136_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8466065.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IV136_002093"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IV136_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDEA010000065        13321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDEA010000065 DACDEA010000000
VERSION     DACDEA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.065"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            286..2025
                     /gene="yfcC"
                     /locus_tag="IV136_002094"
     CDS             286..2025
                     /gene="yfcC"
                     /locus_tag="IV136_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8466066.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2182..3366
                     /gene="iadA"
                     /locus_tag="IV136_002095"
     CDS             2182..3366
                     /gene="iadA"
                     /locus_tag="IV136_002095"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8466067.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3477..3680
                     /locus_tag="IV136_002096"
     CDS             3477..3680
                     /locus_tag="IV136_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8466068.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3781..4443)
                     /locus_tag="IV136_002097"
     CDS             complement(3781..4443)
                     /locus_tag="IV136_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466069.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4456..5958)
                     /locus_tag="IV136_002098"
     CDS             complement(4456..5958)
                     /locus_tag="IV136_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8466070.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6506..8185
                     /locus_tag="IV136_002099"
     CDS             6506..8185
                     /locus_tag="IV136_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8466071.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8312..8791
                     /locus_tag="IV136_002100"
     CDS             8312..8791
                     /locus_tag="IV136_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8466072.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9010..9690
                     /locus_tag="IV136_002101"
     CDS             9010..9690
                     /locus_tag="IV136_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8466073.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9698..11029
                     /locus_tag="IV136_002102"
     CDS             9698..11029
                     /locus_tag="IV136_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8466074.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11044..12006
                     /locus_tag="IV136_002103"
     CDS             11044..12006
                     /locus_tag="IV136_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8466075.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12117..12455
                     /locus_tag="IV136_002104"
     CDS             12117..12455
                     /locus_tag="IV136_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8466076.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12534..13241
                     /locus_tag="IV136_002105"
     CDS             12534..13241
                     /locus_tag="IV136_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8466077.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4276 a         2333 c         2905 g         3807 t
ORIGIN      
        1 caatgaaatt caatttttca atgattgtaa aatgaaaaaa acaggacgag attttctcta
       61 atctaaaaat aaaaaaagaa ttttaaggaa aaacagagaa ataaaatact taatataaat
      121 ggaaagagaa aaaaataatc atacatagga gaaaaaaaac aaaatagaaa aaagtgattt
      181 tctaataaat tattcatgtg gaaatgtcat ttttactgta taattcattt gattatccat
      241 tttttattca tcaaaattac tgaaaaaaga aaggaagaaa taaatttgaa gaaaacaaat
      301 tcttcaggaa aaccgaaacg cgacttcact aaattatcga caccccatac gtatgtcatt
      361 atttttggtg tagtcatctt tgcatggata ctgacttttg tcgttccagc aggaaagttc
      421 agcacgcaag atattgaata caaagatgca aatggcgaaa caagtactcg tactgtttta
      481 cgtcaagatt ccttccgtta tgcttacgag ctggataagt cgtatgtgtt tgatcaatta
      541 gaagaactgc aagatcatcc agcagaacgt gaaaaactgg atgttccgga aaaaggattg
      601 gaaaaagtca ttgctgacgg agaaaaaaat cttacgcagg aaaaactaga tgaaatctcc
      661 ttgacagatg atgtgcttta cgatcagtac ggcgaaaata tctatgatac ttctaaaaaa
      721 ttgcataaaa cagccaaaat atggggaaca gatgattttg gcggctttgg tttcttaaat
      781 tttgtttttg aaggactagt atctggagat aagtatggat ctgctgtagg gatcgctgca
      841 ttgatccttg ttgttggagg tgcttttggt attattatgc gtaccggagc gatcgacgca
      901 ggtatctatg cgtttatcag taaaactaag ggactggagc gtttagcttt accgctatta
      961 ttctttgctt tttcattcgg tggcgctaca ttcgggatgg cagaagaggt aattccattc
     1021 tcaatggtga tggttccatt tgtcattgcg ttaggatatg actcgatcgt agcagtaacc
     1081 gtgacttatg tcgcatctca agtcggaaat gcgacctctt ggatgagccc gtttagcgta
     1141 gcggtggctc aagggattgc tggaatcccc gtgttgtctg gtgcgacatt ccgattgatt
     1201 atgtgggtag ttgttactgc attagccgct ggttatatga tgatatatgc agaaaaaatc
     1261 cgtaaaaaac cagaaagttc gttgacttac aaatcagatg attatttccg ttctcatatc
     1321 aaaaagacat ctgatgaaaa taaaaaattc atgcttgggc ataaattgat cttattagag
     1381 atgttagttg tccttgtctg gatcgtttgg ggcgtgactc aaaaaggcta ttacattcca
     1441 gaaatcgctt ctcaattctt tgtgatggga ttagttgctg gaatcattgc tgttcttttc
     1501 aaattagatg gaatgaaagt caatgacatc gcttcttctt tccaatctgg tgcagctgat
     1561 ttagctggta cagcaatcgt agtaggtatg gccaaaggga ttcttcttgt cttgggagga
     1621 tctgatgcat ctgttccttc agctttgaat acgattttgc atgggatcgg gacggcgttg
     1681 accggtgttc cagctgtcat tggtgcttgg gcgatgtaca tcttccaaag tttgttcaac
     1741 ttggttgtta catcaaattc aggacaagca gctttgacta tgccaatcat ggctccactg
     1801 gcagatttag taggtgtatc cagacaagta gcagttcttg cttatcagct tggttcaggt
     1861 tttatggacg cttttacacc cgtatcagcc agcttgattg gtgtgctagg tgttgcacgg
     1921 atcgaatggg caaaatgggc aagattccaa atcaagatgc aaggattcct gtttgtttta
     1981 ggaactattt tcatcatgat tgccattgca ataggtctgc agtagtactg gagacctgta
     2041 aataaaaaag taatccaaac agtagataag gactccaaga accgtcattt tacggtaaaa
     2101 tcaaaggagt aaagatccta tcgtttggat tattttttaa gatcctatca tcagttatac
     2161 ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa ttgaagtgtt tgcaccagat
     2221 tacttaggaa aaatggatgt actgattgcc ggtgggaaga ttttggctgt agaagaacag
     2281 ctagaaggcg gctacgaagg agtggaagta gaagaacttt caggtgaggg aaaagtacta
     2341 accccgggtt tcattgactg tcatttccat attttaggtg gaggaggaga aggcggttat
     2401 caaaatcgaa cgccagaagt cacattgagc caattgacta cagctggtgt aacgacagtt
     2461 gtcggttgtt tgggaacaga cggagaaggt agagatatga ctgcgttgat cagcaaagcg
     2521 aaaggtctgg aagcagaagg aatctccact tatgtatacg aaggttcgta ccgattgccg
     2581 gtaaaaacag taacagattc tatcatcaaa gactttttga caattgataa aattatcgga
     2641 atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc caagttttga agagttcgct
     2701 cgtgcagtag cagatgctcg tgtaggaggc atgttatccg gaaaagctgg gatcatcaat
     2761 gtccacttag gcggaggaaa aagaaagcta gagctattga cacgaatcgt agaagaaaca
     2821 gaaattccga tcacacaatt tttgcctact catgcaaatc gcacaccaga attatttgaa
     2881 gcttgcgtgg catttgccaa acgtggtggc acgattgact tcactgctag cgaagatcct
     2941 gatttttggg agaagacaga tggtgaagtt cgcttcagca aagcactgaa acgattgatc
     3001 gaggaagatg tttctttaga taatttcacg atgacctcag atggacaagg aagcttgcct
     3061 tattttgatg aaaacaatca tttcctaggg ttaggagtag gaagtgccaa ggcattgcta
     3121 gtagggatca aagaagctgt gcaaaaagaa agtatccctt tggaaatcgc cttacgagct
     3181 ataaccagta atccagcacg tatattgaaa ttggataaaa aaggaaaaat cgaaattggt
     3241 gctgatgcag atctgtgtat cttggataaa gaaacgcttg atattgatac agttatcgct
     3301 aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat gggggacttt cgaaaaaagt
     3361 ttttaatcaa cagctctaac tatctttttt agaaaacgag cgttctgttt gtctgtatct
     3421 gttgttgatg ttaccctgaa ggtagatcaa taaacaaact aagaggagga agcacaatgg
     3481 cagatttaaa aggacgttct aaagatatga aagataaagt agtcggtgaa ggaaaagaag
     3541 catacggcaa agcaacagac gacaagagca aagaaacaga agggaaagca caatcactag
     3601 gtgctgacat caaagaaaaa gctcgaaatt taggcgacga agtcaaagac aagttttctc
     3661 gaaaagacga aaaaaaataa aaaccactta ttactaattg aatcaagcaa ataaaaaatc
     3721 cgaaccatat ctgcgagaca gatatggttc ggatttttta tttgatcatt atcctgtttt
     3781 ttaaacttcg attttttgaa attttttatt ttttagttca aagacttcat cacagctttt
     3841 tgctacttca atctcatgag tcacgatgat aatacagcga ttttgttcat gggcgatttc
     3901 ttggaaaagt ttgacgatat cttgtgaagt tgtttcatct agatttccag taggctcatc
     3961 cgctacgatt agttgatgtt cacagcagat agcacgaacg atagcgactc gctgctgctg
     4021 acctcccgaa agtttggtca cctgtttttt tgctaatatt tcatcaattc ctacacgagc
     4081 cagtcgatct aaagcaactt gtcgataatt ctcttgcttg gtcttagtga tttccattgc
     4141 tgctatgacg ttgtctagag ccgataaata aggaagcaag ttgtaagctt ggaaaataat
     4201 cgagacatca tttttacgat aacttcttaa cccgattttg ttcagtgaac gatcttgata
     4261 gtataccttt ccttcttttg gtgcatctag tccagaaata agcgaaagaa aagtcgtttt
     4321 tccagaacca cttgctccta aaatagcgta catttttcca gcttcgaaat tcagttctac
     4381 atctttataa agcgcatctt gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa
     4441 catcaaattc ctcctttagt ttaataggat ttttcttggg tttaatctta atatccctat
     4501 agaagctaaa aagacagaaa acagactgat tccaaatgct actccggcta atagtcccaa
     4561 ttgtgcaggt ttgacattga tttccaattc gctgacttga tcagaaacag caaatggatt
     4621 attcgtggtt ccttgttgac catcgcgttg accgccttgt tcttgtctgt tacctggcat
     4681 ttgtccaccg ccaggagctt gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag
     4741 ctgctgtccc acggcattcg cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag
     4801 aatcatacaa atagccactt cggtaaacaa ttggagaatg attttacttc tggattctcc
     4861 taaagatagc aagacgccta actcatgccg acgctcccga atggtaatca taatgatcag
     4921 agtaagtatg ataataccgg ctacagtcac tagtaaaaca acatttttag aaaaacttgc
     4981 cacgttgttt aaaggttcca acattgactg atacatggaa tcatttgatt gtaagctaaa
     5041 tgtatctgtg tcgatcagct tctcagcttc tttgagaaac tcatccattt tatctggatc
     5101 agatagagtg taaatagcag aatctatcgt gttatcgtca gatgtcccat ttagttcatt
     5161 tgcaaaagtg taagaagtgt aaagtgtatt cgacggattc atgaagttga atttcatacc
     5221 catactgccg cttgtttcgc tggattcata aattcctttg atcgtcattt cataagtggt
     5281 atcttcatct tcagtactcg tgattgtgaa agtatctccg actgccaggt catttgtttc
     5341 tgctagagta gaatcaatga ctacattgtt tgtgcctttg tcgctgtcat ctattccttc
     5401 accatccgtg atttttgctg ttccatcaga aaaattacta ttttgtgcag ttgctgacac
     5461 accactgatt tgaaagtctg cttgagtcat ttgcggattc ccgcccattc ctccagcagg
     5521 ttcaccgcct gcatcagtag tttctgaact gtcttcactt gaatcagaac tagtgatggc
     5581 agttattcca tcttttgcca tagctgaggc ggaactttca aaggaataac tctttacatt
     5641 atctaattca gctatttttt ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc
     5701 tggatctgga cgttcttcag tggtagaacc gcttgaagaa gtttgttcct gttttttaaa
     5761 tgaagcttct cgattggcac ttaaagtcac tgttgctcca acttcttttt gtgcttgatt
     5821 cgctgcttct tctgcagcgc ttcgaatcgt gaggccagct agcacaaaaa taagaatggc
     5881 tgtgaacacg gcaaacaata acaatgaacg tccccatttt acttttgtac ttttccatgc
     5941 acgtttcata aaattcatat tttgcatccc tcctagtaga tgcatttgat aatagacaac
     6001 ctatcttaaa gctagcttaa atcttatgct gtttgcacac catcaacatg aggttctgta
     6061 tatataaaaa gtcactatag ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc
     6121 aaaccgacga agaagaaatg aaagaggaat agcaaaagaa atcctagatg ctacagaggg
     6181 atttttacgt gcatagttgt tctaacaatt gctgatacta gcgatttagt ttatttgttg
     6241 tataataatg gggaacagct gaatacttat ataagtattc tggctatagc gtttcgcaaa
     6301 agatccattt tgttaatgaa gtatatccaa aataaaatgc ccatccaact gtcagaacgc
     6361 ggatgtgctg gtaattttcc tgctgattct ccataagttg agaaatagtg attttgcata
     6421 tgcttcattg gaatggctca tatatttaga taaagaagaa aatcgacatc tttaaacttg
     6481 gatgtcataa gaagaggtga agtttgtgag taccattaaa attatcccat taggcggcgt
     6541 tcgtgaaaat ggaaaaaaca tgtatgtcgc agaagtgggc gaggatattt ttattttaga
     6601 ttgtgggcta aaatatcctg aaaatgagtt gttggggatc gacgtcgtaa tccctgattt
     6661 tacgtattta gaagaaaaca ttgatcgtgt agctggtgtc tttttaacac atggacatgc
     6721 agatgcgatc ggggcacttc cttacttgtt gtcaaaaatt tctgtaccag tattcgggac
     6781 agagttgacg atcgagttag caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt
     6841 taaggatttc catgtggtag atgagcatac agaaatagac tttggtgaaa cagtagtgag
     6901 ctttttccgt accactcata cgataccaga ttcagtgggg atcaatctga aaacaaaaga
     6961 aggtagtatc gtttatacgg gagattttaa atttgaccaa acggctatcc caatgtatca
     7021 aacagattat gcacgattgg cggaaatcgg caaagaagga gtcttagcct tactaagtga
     7081 atcggcaaat gcagaaaatc ctcagcctgt agcttctgaa gcccaaatag cagatgaagt
     7141 atttgatacg atcaaatatt gggaagggcg tatcatcgtc gcttgtgtag caagtaattt
     7201 gcaacgagtg caacaagtat tggatgcagc agaaaaagca caacgaaaag ttgtcttgac
     7261 tggtcaagat ttcgaacgaa ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc
     7321 aagtgaagat ctgcttgtta cgttgaaaga catgaaaaac tatgatccag aagaactgat
     7381 cattttagaa actggccgaa tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg
     7441 tacacaccgt aatgtcaaga ttgaagaagg agatttggtt tatatcacta ctacgccaag
     7501 catcgcgatg gaaaccatcg tggcgaaaac ggaagatatc atttatcgag ctggtggaac
     7561 agtgaagttg atctctgaga atatgcgcgt ttctggccat gccaatccta atgacttgca
     7621 attaatgatc aatttgatga aaccaacata ttttgtgcca gttcaaggag agtatcgtga
     7681 attagctgct catgcagatt tggctcatgc agtagggatg ccatacaaaa atatctatat
     7741 tactggacgc ggagacgtgc tggaatataa gaacaagcgt atgagtgttg caggaactac
     7801 aagtgcagaa aatgtaatga ttgatggatt aggcataggt gatatcggaa acattgtttt
     7861 acgtgatcga aaaatcttgt cagaagacgg gattttcgta gcggttgtca ctatcagtcg
     7921 gcgagaaaag aaaatcattt caaaacctca aattacttca cggggattcg tttacgtaaa
     7981 agctagccgt gatctgatga aagaaagcag caatatgatt gaaacaatcg ttgagaaaca
     8041 tttagaaagc aatgattttg agtggagcaa attaaagcaa gacatccgag atcaattagg
     8101 ccgttactta tttgaacaaa caaaacgccg cccagtgatc ttgccagtca ttatggaagc
     8161 tactcaacgt cgaaataaaa aataaacaaa aagagtaaga tgaacctctc tatagagtgc
     8221 tcgtcttgct ctttttttgc gaaaaacaaa taatttttga atacagcatc cctcgcttta
     8281 taataaacga tagaataaag ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta
     8341 tttagaagaa caaaagatca gctatcaacc acttgatttt agtcatgtgg tagaagaatc
     8401 tttcacagaa gaactacaaa aaagagggat tgatccagct ttgatatgta aaactctcgt
     8461 agtgaaagga aacaaaactg gggtgatcat cgcattgatt cctttgaacg atcacttaga
     8521 ttacaaaaaa acgagacaag tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt
     8581 tgtattaatg catacaggat atccccacgg agcaaacacc cctattggaa taaaactcgc
     8641 acatcctgat tattgttttt tagcagacag ttctttaaaa gaaaaagaag aagtaattgt
     8701 gtcaagcggt gaaatcggga gaagccttcg gataaaagta gccgatttag aaaagatcgt
     8761 ccatcctatt tacgctgatt taattagtta agggacgaga agagaagcta agcagagcag
     8821 agacctcaaa cttgagatta tatctgcttg gtacttggtg catcctgtat tatctgttac
     8881 aattgctttg tacatactgt actatcaatc caatgaaaat atatttagca gctatatttt
     8941 ttcattgatg tgcaagtgat gaaatcgttt ttaataaaaa agatacagag aaaacgcgag
     9001 gtgtaaaata tgaaaattac gtatcatgga cattcctgtt taacaattga aatggaaaat
     9061 ggccaacgcg tgatgattga cccgtttatt acaggaaatc cattgacaga cttaactgca
     9121 gaagaagtaa agacagattg gttgctgatt actcatggtc atagtgacca cataggagat
     9181 atgattccta ttgcaaaaga aaatcaagcc acaattatca gtattgttga gattgctgat
     9241 tatgcgaaca gcagaggagt agattcattc ggtatgaata tcggtggaaa acatgcgttt
     9301 ccattcggcg tggtaaaatt cgtacatgca cagcatagtt caagctatga agtagacggt
     9361 atcgttcagt atatgggcga gcctagcgga attattattc aagcagaagg aaaaacgatt
     9421 tatcatgcag gggatactgc ttatttcagt gatctaggat tgcttgcaga agaattcgat
     9481 attgatgtcg ctttcttgcc gattggcgat aactatacga tggggccaga agatgcagca
     9541 aaggctgcgc aaacaattcg tgccaagaaa gtagttccaa tccattacaa tactttccct
     9601 gtcattgaac aagatcccga atcttttgta gagttactgg aagatagtga aggaaaaata
     9661 atgagcgtct cggaaacgct ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat
     9721 tttagaatat attgaaagtt tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa
     9781 agaattgaat gtcagtgaag gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg
     9841 actagtttca acaattcaac gagtagggac catccgtatc gaacgcaaat taaaaaaaca
     9901 tattgaacgt ttgacatttg gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg
     9961 ggcggcagga ttagacaagg tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc
    10021 catggaacgc tatattacgc caggatcact aatgatcgtc ggcaaccggg cggaagtcca
    10081 aaaattagca ttggaagatg gcgccgcagt attgatcaca ggaggattca atacgactgc
    10141 tgagattgca aagctagctg atgatcttga aatgccgatc ttacgcacct cttatgatac
    10201 atttacagtc gcaaccatga tcaaccgagc gttaagcgac caattgatca aaaaagatat
    10261 catgcttgtc agtgatatct atatgccagc tgaaaagact cattatttgc agcagacaga
    10321 tacgatttct gattatcagc gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt
    10381 caaccgccat catcgtttga tggggattgt tacggcaaaa gatgttttag ggaaatcgcc
    10441 aaaccagatc atcgatcggg tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag
    10501 tattgcctct gttagccatc aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc
    10561 cgatgacttg acattggaag gcttgattac ccggcaagat gtaatgaaag cgatgcagct
    10621 tgtccagcga caaccgcaga ttgcagatac gatttcggat cagatatctg gaaatatcca
    10681 tacgattgat actgaccgtg aaggaaatcg tttggaaaac cctaagttca aattcgtagt
    10741 agcaccacaa atggtcaatg gcgttggaac catttctttt ggcgtactta gcgaaatcat
    10801 ttctgatgtc gcacaaaaaa caatggtgat gaaccaaaaa cgaaatatct tgattgaaca
    10861 agtgaaccta cattatttgc ggctgatcca attggaaagc gaattagaca ttcgtccacg
    10921 tgtattggaa atcggcagac gttccgctaa actggatatc gaagtgttta ttgaaaacac
    10981 catcgtttcc aaagcaatcg ttgtttgtca agtaatggaa cgttcttaga aagcaggagt
    11041 gaaatgacag tacagcaaga aatagtagat acaataaaaa aatacgataa aatcattatc
    11101 cacaggcatt tgcgccctga tcctgatgca ttaggatcac aagtaggatt agccgaacta
    11161 ttacgggaaa gtttcccaga aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt
    11221 cgctttttag cagaaatgca agaagttgaa gatgtcttct accaaggagc gctagtcatc
    11281 gtgactgata cagctaattc acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa
    11341 ctgatcaaaa tcgaccatca tcccaatgat gacccttatg gagatcttgt gtgggtaaat
    11401 acaaaagcaa gtagctgtag tgagatgatt gcagaatttg ctttgatgtt tcctgaatct
    11461 ttaaagatga acgttaaggc tgcacggtta ctttatgcag gaatcgtcgg agatacaggg
    11521 cgctttttgt atccctccac gacgtcccgt acattagaga ttgcttcgat attgagaaat
    11581 tatccatttg atgcaagtgc tttgaacaga gagattgaac aaatgccgat gaaagtcgct
    11641 aaactttccg gttatcttta tcaaaatatc caggtggatg aaaatggtgc aggaagaata
    11701 atccttccgc aagaaattct tgataaatat ggaatcgatg attcagagac agcttcgatc
    11761 gtttcacttc ctggagtgat tgacgaagtg ctggcttggg ggatttttgt tcagcagcct
    11821 tcagggtatt accgcgtacg tttacgttct aaggggccga tcatcaatga attggctaaa
    11881 cgccatcatg gaggaggcca tccattagca agtggtgcga atgcaaaaga tttagcagaa
    11941 gtagaggaaa tctataaaga gatccaagat atatgccaat cgtataagag cagaaaaaca
    12001 gaatgatact tcgttcgaaa acgtgccttt tgtagtaaaa atcacaaatg tatcttttca
    12061 agatcttctt tttccgttac aatagtgctc actaaagatt ttaaaggaga aagaagatga
    12121 cattaccaaa ttgcccagaa tgtggctcag agtacacata tgaagatcga ggcttgttga
    12181 tttgcccaga atgcgggaac gaatggaaca aagaagaaca agaaactgtc atagaagacg
    12241 gattagtggt attagatgca aatggaaacc gtctgttaga gggcgatagc gtgacagtga
    12301 ttaaagactt gaaagtaaaa ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga
    12361 acatccgttt agtagaaggc gatcataata tcgattgcaa ggtagaagga tttggaccga
    12421 tgaaactgaa atcggaattt gtgaaaaaga attaaagaat attcaaggtc ttttcacatt
    12481 cttttcctac tactaatgat aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg
    12541 gttatggaat gtatggtttt ttagatccga catttctttt ggtcattctt ggattagtta
    12601 tttccggaat tgcgtcagcg tacgtcaata gtactttccg aaaatacgat caagtgagaa
    12661 gcaaaaacaa tgtgactggt acacaagcag cacaatatat attacaaagt caacagataa
    12721 ataatgtcgg tgttcaacaa attgccggag atctcacaga taactacaat tcaggtaata
    12781 agatgttgag tttgtctcaa gcaactgcac agtctacttc agttgctgcg ataggtgtag
    12841 cagctcatga atgcggacat gcggtacaag atgcttcggg ttatgtccca ttgaagattc
    12901 gtgcagcact tgttccagca gctaactttg gttcgatgat ttcgttcccc ttgattatgg
    12961 taggtgtatt gcttagctgg aactctacgt taatcaatat cgggattttc gctttttctt
    13021 tagcattact tttccaattg gtgacattac cagtcgaatt taacgcgtct cggcgagcaa
    13081 tccaaatttt gagtgaaggc ggactactga cggaagaaga agtaccgatg gcaagacacg
    13141 tattatttgc agcagcactg acttatgttg cagcagcgct ttcgacattc ctgcagctgt
    13201 tgcgcttgat cttattgttc ggcggcaacc gaagagatta aaataaagaa gttgtgaaat
    13261 cagcgctttg atctgagcta ataccgagag ttagctgatt gaataccatt tatttggaat
    13321 a
//
LOCUS       DACDEA010000066        12927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDEA010000066 DACDEA010000000
VERSION     DACDEA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.066"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..1383
                     /locus_tag="IV136_002106"
     CDS             82..1383
                     /locus_tag="IV136_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466078.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="IV136_002107"
     CDS             1410..1670
                     /locus_tag="IV136_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8466079.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="IV136_002108"
     CDS             complement(2669..3904)
                     /locus_tag="IV136_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8466080.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            4472..5299
                     /locus_tag="IV136_002109"
     CDS             4472..5299
                     /locus_tag="IV136_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466081.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(5572..6342)
                     /locus_tag="IV136_002110"
     CDS             complement(5572..6342)
                     /locus_tag="IV136_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8466082.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            6560..7627
                     /gene="ulaG"
                     /locus_tag="IV136_002111"
     CDS             6560..7627
                     /gene="ulaG"
                     /locus_tag="IV136_002111"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8466083.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7645..8106
                     /locus_tag="IV136_002112"
     CDS             7645..8106
                     /locus_tag="IV136_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8466084.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            8126..9610
                     /locus_tag="IV136_002113"
     CDS             8126..9610
                     /locus_tag="IV136_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8466085.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            9653..9952
                     /locus_tag="IV136_002114"
     CDS             9653..9952
                     /locus_tag="IV136_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466086.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            9967..10605
                     /locus_tag="IV136_002115"
     CDS             9967..10605
                     /locus_tag="IV136_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8466087.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            10610..11470
                     /locus_tag="IV136_002116"
     CDS             10610..11470
                     /locus_tag="IV136_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8466088.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            11460..12170
                     /locus_tag="IV136_002117"
     CDS             11460..12170
                     /locus_tag="IV136_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8466089.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            12402..12836
                     /locus_tag="IV136_002118"
     CDS             12402..12836
                     /locus_tag="IV136_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8466090.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         4103 a         2029 c         2722 g         4073 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa
     4141 aaataaacat gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg
     4201 tcattataca cacgtaagaa agaaatagtt atgtttaaaa atattcttag actttctcgc
     4261 catgacaatt ttcactatgt gttataactg taaacgataa ttattcagat aacacaaaaa
     4321 gaaatcaagg agcagatacg ccagttgttt aatttgaata ttattttaaa attaaaattt
     4381 ttattctgtt ctaaacattt ttggttgttt agaacttttt tttgtgtatg atataaagga
     4441 gaaataattt tttaaaggga aggaaattgt tttgattaaa gtagatggag cattactaaa
     4501 agaataccga aagaaaaagg ggttaacaca agtacaatta gcagaaggca tttgtgaaca
     4561 agcaactgtc agcaatatcg aaaacaagaa tatgtcgcca ggcatcaata tcttaagtgc
     4621 gctttgcaaa aagctgaatg tcgagatctc cgatatatta gtagaaacag aagaagacaa
     4681 aatcaggaaa aaaataaatg agattcaatc attgaattct gtatcaaaat accaagaatc
     4741 ttataaaatt ttacaaacga tcgatcagga aaaagtaagt aattctgatc ttttaaatca
     4801 aattttattt tatcaaggac tgcttgatca tctttatgcg aaaaaaccag atgcagcatt
     4861 attttatttt aatcaagtgt tggaacaaac gaccgaaaca gatatccatc acttacaagc
     4921 agctgcaaac gtggcaatga tctacttgtc aaaaggagaa ttagattttg cgaaagttta
     4981 tgtggagcgt acacttaaaa tattgagtga gactgatttt gataatctga tggtctgcat
     5041 tgtatattat gatatcgcca cctattaccg taaaatcgag gattatgata aagcaatcca
     5101 attatgtgag aagggtattg agtataataa gaaacataaa tcgacttatg cattggaata
     5161 cttgctttat gaaattgctt cttgccataa gcaattagga gaagatgatt atttagaaag
     5221 atatatggac gcaaaaaaaa tagcgcgttt caacggaaat gattacgcgg taaaggtgat
     5281 tgaaaacgac ctgaaataaa gtggttgtga aatcagtgtt ctgatttgaa taccgtttat
     5341 tcgaaaaaag gaggtgtgac ttttgtcata cctccttttt tcgatattgt ttgattccta
     5401 tttaggcaac atataacggt tataaaagaa gcaatagatt atgatggtat cgattggatg
     5461 gatacatctt gggattcctt tatcaatttg agcgtagcgt gtttttcatt tatcgataaa
     5521 tttgttattc atttttttga taatcctatt tacatattta cgtcctggtt cttaaaatga
     5581 atctacttga atggttttta tccccgtatt tctgatctcc tctaatactt tttcagaaca
     5641 aaatttatca gtgatcaaat aatcaaagac tgaaatatca gcaatcttga actttgatgt
     5701 cagtccgatt tttcgatgat cagcgactaa aatcttacat ttgattgtct tgttgaccat
     5761 catttcgttg atcgttgatt catttaatat ctttgttgtg accccatgta ctacatcgac
     5821 tccgctacag ccaatgatac aaatgtctgc attgactgaa gaaatcgtat ttaatgcgat
     5881 gtcgcccact agtgcttctt ttggaaagcg catctctcca ccagttaaaa tataactaca
     5941 attatctttg tgcggagagt aattgatttt cacgttgttt gtaatgactg tcaaatggtt
     6001 agattgaaga aaatccagtg ttttcaacgc agtcgagcta gaattgataa acagcgtatc
     6061 ataatcgtta atgtatttac tagcttctct ggcaatcgct tgtttaatcc tttcaggacc
     6121 agattgatca aaatcagtct ggtttttgta attataagaa caataaccgt aattgcgtat
     6181 gatctcgttt ttttcttcta ataaatttaa atctcttcgt atcgtactaa tagatacatc
     6241 aagggattca gctaattcga aagttgatag ttgttcagtc tgctgaagca gttccaaaat
     6301 ttctttttga cgtttttcaa tattgacaag tgagtttttc atgacttttg cctcctgagg
     6361 gacatcttga tccatggatt tatttgacta gttgttcatt taaacattca tatctgtctt
     6421 tattatactg aaacaatcat tgattactga aagaaaatca tataaaaaac aaacggtcat
     6481 ataaaagggt tttcatggat gctgatacac attaatattt acttgtaagt tggaacaacg
     6541 atatgggggg agcaagcaaa tgagtaaaac tgtcaatgaa gtaacaaaag aaaagtggtt
     6601 agtagaaaca tttcctgagt ggggcacata cttgaatgaa gaaatcgaaa cagaagtagt
     6661 ggatcctgga acttttgcta tgtggtggct gggctgtacg ggaatttggc tgaaaagtca
     6721 tgaaggaacg aatattttat gtgatctatg gtgtgggacg ggaaaacgta gtcatgggaa
     6781 tggcatgatg aaaaaaggcc atcagatgat gcgtatgagt ggcgttcaaa atatgcagcc
     6841 taatttacgt actcagcctt tcgtaatcga tccttttgcg ataaaaaatg tagacgcttt
     6901 aatcgttaca catatccatt ccgatcatct agatatccat acagccgcag cagttcatca
     6961 aaattgtcct gaagcaacat tcattgggcc aaaagaagtg gttcgcacgt ggcaaaaatg
     7021 gggaattcct gaagaaaaaa cgattacagt aaagccaggt gatgcagtca aaatcaaaga
     7081 catcaatgta gtagctttag aagcgtttga tcggacagcg ttggtcactt gtgaagatcc
     7141 agaagaggta ttaaaaggaa aaatgcctca ggagatggat caaattgcag taaactattt
     7201 attcgaaaca tcaggaggga atctttatca tgcaggagat tcacattact ccaatttatt
     7261 tgccaaacat ggtaatgaac acaaaatcga tgtctgttta ggggcctatg gtgaaaaccc
     7321 acgagggata acagacaaag ttacttctgt tgatatgctg cgcatggcgg agtcattgaa
     7381 tgcaaaagta gtgattcctg tccactacga tatttggtct aattttatgg ctgatccaaa
     7441 agagatcact gaaatttgga aattcaaaaa ggatcgcctg aagtatggat ttaaacctta
     7501 tatctggcaa gtaggcggga aattcatcta tccgacaaac aaagatgatc ttgagtttaa
     7561 tttttatcga ggatttgaag atgtcttctc gattgaaaat gatacaccgt ttccgtcgtt
     7621 tttataagaa agaaggggag tttcttgctg aaatactttt ttgaccatca attgattcat
     7681 tacccagata aagaaatcac ggattggcgt gaagctatcg ccgaaagctg tcttgtattg
     7741 atcgataagg aaattattga tgaaacgtat gtggaggaga tcatccattg tgtagaaaaa
     7801 tacggtcctt atattgtgat cgcacctcaa gtagcaatgc cgcattcttc agaaaaaagt
     7861 gatggtgttt ttggaacagc tatttcattt accaagttga aacagccaat ccgttttgaa
     7921 agtgaagggg agaatgagga gaaaacggct agtttgtttt ttacactcgc agcaaaagat
     7981 ccagaagaac atctagaaaa tatccaaaaa ttatccgaat tattaatgac agatggattg
     8041 gttgagcgtc tcatctctac gaactccatc gatgattacc aagttgtgat ggcagaatat
     8101 aattaattga tagaggagaa aaaagatggg ggaatttttg ttaagcattt ggacgtattt
     8161 tgcaacaaat atcttgacgc agccagcata cctgattggt tttatcgtgt tattaggcta
     8221 tattttgctg aaacgcccgc ttcatgaatg catcgctgga ttcttaaagg caacggtcgg
     8281 ttactttatt ttgactgttg gctctggtgg tttggtcaat aattttcggc caatcttggt
     8341 tggactaaag gaacggttca atttggatgc catggttaca gatccttatt ttggacaaaa
     8401 tgctgtggat gctggattaa tggagacatt tggccgtacc tttggagatg tgatgatttt
     8461 attactgatt gcatttgttt taaatatctt attagtccgt tttcagaaat atacgaaact
     8521 gcgttccgtt ttcactaccg gaaacgttca ggttcaacaa gcagctaccg ctttttggat
     8581 tttactgttt tgctttcctg atctaggtag gatacaagca ttgattttta tgggactgat
     8641 tttaggttgt tattggacag tagcttctaa cttaacagta ggaatcacgc aggaattgac
     8701 agaaggtgcc ggatttgcag tggcacatca gcaaatgttc ggaatcttta tatttgcaaa
     8761 attagctgag tggatgaaaa agcgggatga aaagaaaaat agatcaatca agcaagacaa
     8821 aaaaattgaa gatatcaaat taccaggatt cttgtcgatt ttcaatgaga atatggttgc
     8881 tacttcgtta ctcatgctat ttttctttgg gatcattctg attgtactag gcaaagatta
     8941 tttgattcaa gcgcaattca tgcaagaagg gcagagtttc ttattctaca ttatgacgac
     9001 ttctttaaat tttgcggtat atctggcgat cttgcaatta ggtgttcgga cattcgtcga
     9061 tgaactaact caatctttcc aaggaatctc aaacacgatt ttaccaggag ctgttccagg
     9121 aattgacgta gctgctactt ttggctttgg ttcgccaaat gcggtaacga ttgggttctt
     9181 gtttggtgct ttgggacagt ttttgatgat tggtttactg attttattga aatcaccaac
     9241 gatcgtcata gccggtttca ttccgttgtt ctttgataat gccgttatag cagtttatgc
     9301 caataaccga ggtggattca aagccgcttg cctgttcccg tttctttcag gaatcataca
     9361 agtaggcggt tcagcattat tcgctacatg gattgggatg tcaacatacg gtggttatat
     9421 cgggatgttc gactgggcaa cagcttggcc attgtttaca gtaatcatga aagtcctagg
     9481 ttatgctgga attgccgtag ttgtcattgg tttattggct attccgcagt tacaatatcg
     9541 gaaaaatcct gatggttatt ttttgattgt agaagactat gacacatatg cagaaaagca
     9601 gagagtataa aagaaaatct gattagacaa cactcataat ggagggatta agatgagaat
     9661 tttagtatcg tgtgcaaatg gatcaggaac aagcttgatg atgatgagaa gtgtggaaaa
     9721 agcaatgaaa gaactaaacg taccaattac caaaatccat cattgtgcca tttcagaggg
     9781 aaaaagttca gctggccaat acgatgtagt ttttacgcca gtcaattttt tgaatatgtt
     9841 tactcaggca gagaaaaaag gagttacagt tatcggtatc aaaaatgtga tgtcagtcaa
     9901 agaaatcagt gaaaaatttg ctgaaacaaa gtggtaccaa aaaaacaagt aaaggaagga
     9961 actaagatga gcaggccgaa tttgcaaatt gcgttagatc acaatgactt agaacatgca
    10021 ttaggagatg ttatgaaagt tggtgatgta gtagatatta ttgaagtggg cacaattctt
    10081 tgcttacagg aaggacaaaa agcgattcgc tgtattcgca gtatgttccc agataaaaaa
    10141 ctagtcgctg atacgaaatg tgcagacgct ggaggaacag tggcgagtaa tgttgcaaaa
    10201 gcaggagcag actggatgac agtcatttgt tgtgcaacga ttccaactat ggaggcagca
    10261 caaaaagaaa taggtgaatt gcaagtagaa ctctacggca attggacttt tgaacaagcc
    10321 atggattggc ataacatcgg tatccggcag gtaatctacc atcaaagccg tgatgcgctg
    10381 ttatctggtg aaacttgggg tgaaaaagat ttatcgaaga tcaaaaaact gattgaacta
    10441 ggctttaatg tttctgtaac tggtggatta aatccacaca cattacactt atttgaaggg
    10501 atcgatgtgt atacctttat cactggtcgt ggtattacag cagcaaatga tccgatgaag
    10561 gccgcacaaa acttcaaaga tgagatcatt cgtatctggg ggtaaggaaa tggctcaaat
    10621 tgggatttat gaaaaagcgt tacccaaaga tatttcatgg aaagaacgtt tttcccttgt
    10681 aaaagaaatg gacttcgatt ttatcgagat gtcgattgat gagacggatg agcggttagc
    10741 acgattagat tggtctgaag aaaagatagc tgagctgaga gaagagatgt tcagctccgg
    10801 tgtacgtatc cattcgattt gtttaagtgc gcatcggcga tttccgtttg gttctgctga
    10861 tccagaaaag aaaaaagcag cgaaacaact aatgaaaaaa gcaatccatt tggcgcataa
    10921 tctaagcgtc aaagtaatcc aaatagctgg atacgatgtt tattatgaag agaaatcaat
    10981 gaattccaga gaagacttta tcgaaggaat aaaagaaagc gtaaaggaag cttctaaata
    11041 tggcatcatt ctttccgtgg aaatcatgga tgatccattt atgaactcca tttcaaaatt
    11101 tttagaaatc aaagaacaga ttcattcacc ttttctacaa gtatatccag acctaggcaa
    11161 tttgtccgca tggccggaaa ataatccggc tcgagaactt gagaaaggga tcgattatat
    11221 cactgctatc cacttaaaag atacttaccc tgtaacaaaa gaatattcag gccagtttcg
    11281 tgatgttcct tttggtagcg gttgtgttga ttttcttgga ttattaagac atttgaaacg
    11341 tctagattat gatggaacat ttctgattga aatgtggagt gaaaaaagcc aagactttcg
    11401 aagagaaatc cagcaggcaa aagactactt aagttctaaa ttgaaggagg ctggctatga
    11461 tgtcttgtga agaaaaaatt gcagcaatga agcaacgtgt ccttgaagca aatctggcat
    11521 tgccgcgata cggattagtc aaattgactt ggggcaatgt gagtgaagtg gaccgtgagc
    11581 taggagtcat cgtcattaag ccaagcggtg tcagatatga atgcatgcaa gcagatcaaa
    11641 tggtggtaac agatttgtcg ggaaatataa tagaagaaga tagcttaaaa ccatcttcag
    11701 atctccccac acatgttgtt ttgtatcaaa cttttgaaga tataactgct attactcata
    11761 ctcattccac gcactccgtc atgtgggcgc aagcaggacg cgatttgcca gcttatggca
    11821 cgactcatgc tgatgctttt tatggtaagg ttccttgtac acgccaatta acgaaagaag
    11881 aagtaagaga agcttacgaa gttcatacag ggaatgtgat tgtcgagacg tttaaggaaa
    11941 gaaaattaga tccaaacgaa gtccctggag tacttgtcta tggacatgga ccgttcacat
    12001 ggggggattc acctatgaaa gcagtagaaa acagtttgat cttagatgaa atttgtctga
    12061 tggccaaaga aaacgagctg atcaacccca atatatgtga aatcccgcaa tatttactag
    12121 ataaacatta ttttcgcaaa catggaaagt atgcatacta cgggcaatag agatgctagg
    12181 gtaagggtag aaaattaaaa gataacactt gatagagaga aacagaggtt gtgaagaaaa
    12241 tttatcacaa cctttttatt tatcaaaaag tatgcattct aaaaggaaag aggaatttta
    12301 aggaatcgag agaatcttta caggagagtt atatgtgata gattaaatag tcaaagaact
    12361 gtattattag agggagaatt gtataagaaa ggaagaaaaa aatgaatatt gattccctta
    12421 tgatcgatga gatttctgaa ttatataaga gcgttggttg gacgaattac acaaaagaca
    12481 ctgcaaggtt agctagagca tttgaacaat ctgaatctct gataaagcgt aatggggaag
    12541 ggaagattat cggaatcgtt cgttggatca ctgattttgc gacgatcgca tttattcaag
    12601 atatcctcat tcatccgagg tatcagcgcc aaggaatagg caaagtgctt ttgaatgaag
    12661 ctttggagaa gatcactagt tatggaccag tccaaatcga attattgaca gatgatactg
    12721 agaaaacaaa aaaattttat gaatcagtag gatttgttca agtaaaggaa atgggtggta
    12781 tttcttatgt catagatact agaagaaaaa aggtgctttt tggtaatgac aaataacata
    12841 taataacgta caaaagaaaa aattctagct tagttcttta gaaaaacaag aggatagact
    12901 tagtatcact caatctatcc tccaatg
//
LOCUS       DACDEA010000067        12738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDEA010000067 DACDEA010000000
VERSION     DACDEA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.067"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV136_002119"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV136_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8466091.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV136_002120"
     CDS             complement(322..891)
                     /locus_tag="IV136_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8466092.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV136_002121"
     CDS             1077..1979
                     /locus_tag="IV136_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8466093.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV136_002122"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV136_002122"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8466094.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV136_002123"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV136_002123"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8466095.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV136_002124"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV136_002124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8466096.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV136_002125"
     CDS             8547..8732
                     /locus_tag="IV136_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8466097.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV136_002126"
     CDS             complement(8794..9990)
                     /locus_tag="IV136_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8466098.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV136_002127"
     CDS             complement(10160..11596)
                     /locus_tag="IV136_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8466099.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV136_002128"
     CDS             11889..12698
                     /locus_tag="IV136_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8466100.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DACDEA010000068        12677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDEA010000068 DACDEA010000000
VERSION     DACDEA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.068"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IV136_002129"
     CDS             complement(287..649)
                     /locus_tag="IV136_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466101.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IV136_002130"
     CDS             complement(723..1103)
                     /locus_tag="IV136_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8466102.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IV136_002131"
     CDS             1185..2360
                     /locus_tag="IV136_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8466103.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="IV136_002132"
     CDS             complement(2436..3020)
                     /locus_tag="IV136_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8466104.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3221..4219
                     /locus_tag="IV136_002133"
     CDS             3221..4219
                     /locus_tag="IV136_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8466105.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4331..4840
                     /locus_tag="IV136_002134"
     CDS             4331..4840
                     /locus_tag="IV136_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8466106.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4870..5529)
                     /locus_tag="IV136_002135"
     CDS             complement(4870..5529)
                     /locus_tag="IV136_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8466107.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5531..6295)
                     /locus_tag="IV136_002136"
     CDS             complement(5531..6295)
                     /locus_tag="IV136_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8466108.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6303..6980)
                     /locus_tag="IV136_002137"
     CDS             complement(6303..6980)
                     /locus_tag="IV136_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8466109.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7017..8402)
                     /locus_tag="IV136_002138"
     CDS             complement(7017..8402)
                     /locus_tag="IV136_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8466110.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="IV136_002139"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV136_002139"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IV136_002140"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IV136_002140"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8466111.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IV136_002141"
     CDS             9341..10198
                     /locus_tag="IV136_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8466112.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IV136_002142"
     CDS             complement(10279..10614)
                     /locus_tag="IV136_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466113.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IV136_002143"
     CDS             complement(10681..12051)
                     /locus_tag="IV136_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8466114.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IV136_002144"
     CDS             complement(12129..12665)
                     /locus_tag="IV136_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8466115.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3766 a         2570 c         2120 g         4221 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca taataataat
     3061 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
     3121 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
     3181 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
     3241 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
     3301 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
     3361 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
     3421 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
     3481 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
     3541 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
     3601 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
     3661 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
     3721 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
     3781 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
     3841 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     3901 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     3961 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     4021 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     4081 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     4141 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     4201 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     4261 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     4321 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     4381 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     4441 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     4501 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     4561 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     4621 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     4681 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     4741 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     4801 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa
     4861 aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa
     4921 aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa
     4981 aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc
     5041 gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa
     5101 aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag
     5161 ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa
     5221 cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc
     5281 agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga gataattcat
     5341 taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg
     5401 caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag
     5461 actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga
     5521 atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag
     5581 gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat
     5641 tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt
     5701 ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga
     5761 tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag
     5821 gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac
     5881 ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc
     5941 ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt
     6001 caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa
     6061 aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact
     6121 catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca
     6181 agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt
     6241 gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc
     6301 cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt
     6361 tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata
     6421 accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta
     6481 tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgt
//
LOCUS       DACDEA010000069        12596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDEA010000069 DACDEA010000000
VERSION     DACDEA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.069"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..904
                     /locus_tag="IV136_002145"
                     /pseudo
     CDS             52..904
                     /locus_tag="IV136_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1001..2887
                     /locus_tag="IV136_002146"
     CDS             1001..2887
                     /locus_tag="IV136_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466116.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2928..3830)
                     /locus_tag="IV136_002147"
     CDS             complement(2928..3830)
                     /locus_tag="IV136_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8466117.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4060..5463
                     /locus_tag="IV136_002148"
     CDS             4060..5463
                     /locus_tag="IV136_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8466118.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5463..6896
                     /gene="ascB"
                     /locus_tag="IV136_002149"
     CDS             5463..6896
                     /gene="ascB"
                     /locus_tag="IV136_002149"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8466119.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7041..7967
                     /gene="trxB"
                     /locus_tag="IV136_002150"
     CDS             7041..7967
                     /gene="trxB"
                     /locus_tag="IV136_002150"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8466120.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8181..9908
                     /locus_tag="IV136_002151"
     CDS             8181..9908
                     /locus_tag="IV136_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8466121.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9981..10274
                     /locus_tag="IV136_002152"
     CDS             9981..10274
                     /locus_tag="IV136_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8466122.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10369..11229)
                     /locus_tag="IV136_002153"
     CDS             complement(10369..11229)
                     /locus_tag="IV136_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8466123.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11321..11749
                     /locus_tag="IV136_002154"
     CDS             11321..11749
                     /locus_tag="IV136_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8466124.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11911..12087
                     /locus_tag="IV136_002155"
     CDS             11911..12087
                     /locus_tag="IV136_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8466125.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12114..12560
                     /locus_tag="IV136_002156"
     CDS             12114..12560
                     /locus_tag="IV136_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8466126.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4120 a         2171 c         2775 g         3530 t
ORIGIN      
        1 ttttcttttt gttttggata cagccctata tatcatggaa aggagcaaaa catgctgatt
       61 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
      121 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
      181 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
      241 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
      301 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
      361 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
      421 caactatttc ctaaagagta tgccatcggg aaaaaaaaca gtgaagaaaa tctcggataa
      481 ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc
      541 acaaggcatt caaagccaac aagacaatac ctacctgttc actataacaa tacaggatat
      601 tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccatttttt
      661 tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga
      721 agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt
      781 tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac
      841 ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
      901 ataaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
      961 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
     1021 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
     1081 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
     1141 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg
     1201 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
     1261 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
     1321 tttccaacca ttccttgcta tcctagctgc tgacgggatg attcgtggac tgacagcatt
     1381 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
     1441 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
     1501 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
     1561 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
     1621 ttttgaaaca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
     1681 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
     1741 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
     1801 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
     1861 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
     1921 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
     1981 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
     2041 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
     2101 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
     2161 gtttacattg ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
     2221 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
     2281 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
     2341 gatcgccatt gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc
     2401 agatgaaaca gatgatagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca
     2461 aggacaaatc gctccactga atgcagcaaa ggatgcagct tttgcacaag gaacactagg
     2521 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
     2581 catgtccctg tttccgacga aacatgcaat cgggatggtt tctgaagcag gtctggaatt
     2641 gctgattcat gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt
     2701 gcagcaagga gaaagagtga aacaaggcga tctgttgctt cgcttcgaca aaaagaaaat
     2761 cgaagaggaa ggatacagtt tagaaacacc tgtgattgtc atcaattaca tggattattt
     2821 ggatattttg gaaaatcaag gggaaacggt cgggccagac gatgcactta tcaccgcact
     2881 gacctgaacg gttaggtgaa agaatggact aggcttaact attcttttta gtccacttct
     2941 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat
     3001 ttttgtccta cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
     3061 ctatataaca cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttc
     3121 gaatacagtc gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga agatagcgtt
     3181 gaatcaccga tactggtcaa agctaaaatt ggcacatgat tttgtttcaa tatttctgcg
     3241 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccaca catatcactt
     3301 gtgcaattgt acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt
     3361 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
     3421 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
     3481 tgaatgagag aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg
     3541 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
     3601 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
     3661 cccatttttt ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc
     3721 tcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca
     3781 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
     3841 tcttcttgtt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
     3901 gttatatagc ttaataaaga cattttttga caagatattt acaaaaatcc agaagaaaac
     3961 actaactttt gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa
     4021 aaaagataat aaaagtgtaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
     4081 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
     4141 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
     4201 cttgagatcg tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa
     4261 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
     4321 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
     4381 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
     4441 gtggttttgg caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgattaat
     4501 ttcgcgaacc caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
     4561 gtgatcggat tatgcaatgt gccagtaatg gcgatgatga cagaaccaac aatgcttggc
     4621 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa
     4681 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
     4741 gacaatggat tgccgaaaaa tatctttgac atcccgtttt tcaaagaaca attaaaacaa
     4801 atgaacatga tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa
     4861 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
     4921 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
     4981 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
     5041 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
     5101 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
     5161 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
     5221 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
     5281 aatccgctta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
     5341 caccagcagt atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc
     5401 gaagtccaag atgaactggc aagagaatta tcaagcaata cacatgtaat ggaggaagta
     5461 tgatgggatt cagaaaagat tttttatggg gcggagctac agcagccaat caatgcgagg
     5521 gcggttatga tgaaggcgga cgtgggttag cgaacgtaga cgttgtgcca atcggagaag
     5581 accgttttcc tgtcattact ggaaaaaaga aaatgtttga atttgatgaa aatcattttt
     5641 atccagcaaa aattgcgatc gatatgtatc atcattataa ggaagatatt gctttatttg
     5701 cagaaatggg gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg
     5761 gcgatgaaaa agagccaaat gaatctgggt tgaaatttta tgaggatgtc tttaaagaat
     5821 gtcaaaagta cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt
     5881 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
     5941 gccgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga
     6001 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa
     6061 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
     6121 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
     6181 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
     6241 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
     6301 aaatggaacg aaatggcatt cagatcgaaa tggaacctgg ggatgccgca ttgctgaaag
     6361 aacatactgt tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca
     6421 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg
     6481 aagcaagtga atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata
     6541 tttatgatcg ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata
     6601 cgcccgatga gaatggttat gtggaagatg attatcgaat cgactatctg gcagcacata
     6661 tccaagcaat gaaagatgca gtagaattag atggagtcga tctattaggc tatacgactt
     6721 ggggatgtat tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca
     6781 tttatgtcga tcgtgacaac gagggaaatg gcacattagc ccgttcgaag aagaaatcat
     6841 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa
     6901 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
     6961 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
     7021 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
     7081 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg
     7141 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa
     7201 tcatgggtcc tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa
     7261 atgcttatgg aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg
     7321 aagataaaac ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat
     7381 taggcgtacc tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg
     7441 atggtgcgtt tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag
     7501 aagaagccat ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg
     7561 aattacgtgc acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta
     7621 tgtgggatac agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag
     7681 cacgtaatgt caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg
     7741 ttggactgga tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct
     7801 ggattccaac agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg
     7861 ttcgtgaaaa agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac
     7921 aacaagtgta taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt
     7981 ggaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatct actgaatccg
     8041 atttatgcga atacttgcaa ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt
     8101 tttggtcctc atacttttat atctaataag tgaatgctat acttattaag aaccatattt
     8161 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
     8221 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
     8281 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag
     8341 ggcctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggacttgcac
     8401 gtttcatgga tacacaaggc gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
     8461 cacgccatca atcaccagaa tttgccatgg aagcagccaa aacattagca catcacaata
     8521 tcccatctta tgtattcgaa agtctacgtc caacaccaga gctttctttt gcagtccgtc
     8581 atttgaagac gtttgcggga atcatggtaa ctgcatccca caatccagca gcttacaacg
     8641 gatataaagt ctacggcgaa gatgggggac aaatgcctcc tgcagacgca gatgcattga
     8701 cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga agtattatct gacgaagaag
     8761 ctgaacatag cggattgatc acgatcatcg gagaagaagt ggatgccgct tatctagaag
     8821 aaatcaaagc agtaacgatt gatcatgaat tggtggaaac aatggggaaa gatttgaaac
     8881 ttgtgtacac accacttcac ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag
     8941 ctggattcga acaattcgta ctagttccag aacaagctgt cgcagaccca gatttcacta
     9001 cagtcaaatc accaaatcca gaagaacact ctgcttttga gtatgcgata cgacttggcg
     9061 agaaagaagg cgctgatttg ctgattgcaa cagatcctga tgcagaccgt ttaggtgcag
     9121 ccgttcgttt gccaaatggc tcctatgaag tattgaccgg aaaccaaatc ggagcactgt
     9181 ttgctcaata tattttggaa gcacaccaat cgcgaggcac tttacctgaa aacgcagcgg
     9241 tattgaaatc gatcgtttct agcgaattac ctacagcaat tgcggaacac tatggggcta
     9301 ccatgttcaa tgtattgaca ggattcaaat tcattgctga aaaaattcaa caatttgaag
     9361 aagatcattc ccatactttt atgttcggat tcgaagaaag ttacggttat ttagttaaac
     9421 ctttcgttcg cgacaaagac gcgatccaag cattgctctt attagcagaa gtagcagctt
     9481 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
     9541 gctactacgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
     9601 tctctgcttt gatggaaaaa ttccgtgaag aagcaccaac agaatttgcc ggagcaaaag
     9661 tcatccaaac agaagacttc aaagagttga cgagaacaga ccgtgaagga aacgtagaaa
     9721 aactaacgac accgccatct gatgtattga agtatacgct ggaagaaaat ggctggatcg
     9781 ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta catcggtgta aaaggaaact
     9841 cttcagaaga cgcagctgaa aaagtagccg cttatgaagc gattatccaa aagatgacag
     9901 aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
     9961 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc
    10021 aactattcaa aatgttgagt gaccccactc gtctcaaaat tctgctgtat ctgaaagacg
    10081 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggaacaatct gctgtatcgc
    10141 atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga
    10201 ttttgtatag tcttgatgat tctcacgtac tggatatttt gaaccagaca ctcaaacatg
    10261 tggaacatca ataacgatag gtgcaaatag agacaaactg gactgtgtca acgaatcttc
    10321 ttcgctgaca cagtccagtt ttttttaaga aaaagacaag aaaaactgtt agtctgtttc
    10381 ttcggtggtt tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag
    10441 cgaagccgtt tcttcaatag ataaagatgc cacattgatc actacacatc ctagtttttc
    10501 ataaagatca ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt
    10561 atcagggttt aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg
    10621 gttattcgtt agtcccacga tttttttagg atcgatttcc cacagctgtt tgggaatatg
    10681 tgcttgggga atcaaaggta aattagcaac ttttaaattt ttatttgcta aaaacaagct
    10741 caaaggcgtt ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc
    10801 acgaggatct ttcccatcat cgtattttac agcaaattcc attgctttga tccgtttgaa
    10861 atagttctca ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa
    10921 acgttcaatt tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt gatgttcttc
    10981 acaaaaagat cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc
    11041 tttttcttct aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt
    11101 tctgcggaat aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt
    11161 tgtcgcagtt tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc
    11221 ggaagacatg gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga
    11281 atgcgtactt cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat
    11341 cgaacaatca ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc
    11401 attgttaact tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta
    11461 tatgaatcag gaattcaaag gggtaagtta tgatacgatt tatcggaatc tccatgattt
    11521 tgcagagatc gatttactgg aagaaaccga tttaaatgga gaaatgaagt ttcgttttca
    11581 ttgctgtcag ggggaaatcg gccaccacca tcattttatc tgtacagtgt gcggaacaac
    11641 gaaagaaatc catatgtgtc cgatgaatta tttcgaagaa cagctaggtg gatgcacgat
    11701 cgaagggcat agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata
    11761 aaataacatt tgataattta aaaaaaaagc aagatttcct tgacggtcaa aacagcatta
    11821 gatataatat ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt
    11881 actttttaag ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa
    11941 tcacttgacg atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa
    12001 gaatctcgta aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa
    12061 gcagctagaa aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac
    12121 tactaagtag attaaacgat gacatgaaag cagcgatgaa agcgaaagat aaggaatctt
    12181 tacaagtcat ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg
    12241 acttgacaga agaagaagaa ctaacggtgt tgtctcgcga aatgaaacag cgacgtgatt
    12301 ctttgcatga gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa
    12361 ttgtaatagt cgaaaaatat atgccggaac aactttccga cgaagaaatt cgacaacttg
    12421 ttcaagaagc aatcactcaa actggtgctt catcaatgaa agaatttggc aaagttatgg
    12481 gtgcaattat gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga
    12541 aagaactatt acaatcttaa ttttatcatt taaactgcgg acagtgattg tccgca
//
LOCUS       DACDEA010000070        12415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDEA010000070 DACDEA010000000
VERSION     DACDEA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.071"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..515
                     /locus_tag="IV136_002157"
     CDS             <1..515
                     /locus_tag="IV136_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466127.1"
                     /translation="HLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYH
                     PDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGK
                     LVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQK
                     QPRREVQHSH"
     gene            complement(660..1400)
                     /gene="truA"
                     /locus_tag="IV136_002158"
     CDS             complement(660..1400)
                     /gene="truA"
                     /locus_tag="IV136_002158"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8466128.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1591..2226)
                     /locus_tag="IV136_002159"
     CDS             complement(1591..2226)
                     /locus_tag="IV136_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8466129.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2373..3614)
                     /gene="sstT"
                     /locus_tag="IV136_002160"
     CDS             complement(2373..3614)
                     /gene="sstT"
                     /locus_tag="IV136_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8466130.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3812..4810
                     /locus_tag="IV136_002161"
     CDS             3812..4810
                     /locus_tag="IV136_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466131.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4823..6841
                     /locus_tag="IV136_002162"
     CDS             4823..6841
                     /locus_tag="IV136_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8466132.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6901..7617)
                     /locus_tag="IV136_002163"
     CDS             complement(6901..7617)
                     /locus_tag="IV136_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8466133.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7844..8386)
                     /locus_tag="IV136_002164"
     CDS             complement(7844..8386)
                     /locus_tag="IV136_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8466134.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8391..8618)
                     /locus_tag="IV136_002165"
     CDS             complement(8391..8618)
                     /locus_tag="IV136_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8466135.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8697..10577)
                     /locus_tag="IV136_002166"
     CDS             complement(8697..10577)
                     /locus_tag="IV136_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8466136.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10766..11380)
                     /locus_tag="IV136_002167"
     CDS             complement(10766..11380)
                     /locus_tag="IV136_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8466137.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11522..12082
                     /locus_tag="IV136_002168"
     CDS             11522..12082
                     /locus_tag="IV136_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8466138.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3914 a         2460 c         1974 g         4067 t
ORIGIN      
        1 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
       61 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
      121 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
      181 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
      241 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
      301 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
      361 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
      421 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
      481 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
      541 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
      601 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
      661 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
      721 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
      781 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
      841 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
      901 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
      961 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
     1021 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
     1081 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
     1141 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
     1201 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
     1261 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
     1321 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
     1381 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
     1441 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
     1501 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
     1561 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca
     1621 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
     1681 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
     1741 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
     1801 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
     1861 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
     1921 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
     1981 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
     2041 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
     2101 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
     2161 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
     2221 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
     2281 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
     2341 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
     2401 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
     2461 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
     2521 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
     2581 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
     2641 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
     2701 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
     2761 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
     2821 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
     2881 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
     2941 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
     3001 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
     3061 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
     3121 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
     3181 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
     3241 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
     3301 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
     3361 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
     3421 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
     3481 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
     3541 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
     3601 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
     3661 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
     3721 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
     3781 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
     3841 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
     3901 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
     3961 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
     4021 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
     4081 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
     4141 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
     4201 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
     4261 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
     4321 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
     4381 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
     4441 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
     4501 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
     4561 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
     4621 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
     4681 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
     4741 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
     4801 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
     4861 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
     4921 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
     4981 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
     5041 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
     5101 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
     5161 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
     5221 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
     5281 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
     5341 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
     5401 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
     5461 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
     5521 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
     5581 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
     5641 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
     5701 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
     5761 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
     5821 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
     5881 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
     5941 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
     6001 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
     6061 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
     6121 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
     6181 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
     6241 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
     6301 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
     6361 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
     6421 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
     6481 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
     6541 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
     6601 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
     6661 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
     6721 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
     6781 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
     6841 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
     6901 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
     6961 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
     7021 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
     7081 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
     7141 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
     7201 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
     7261 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
     7321 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
     7381 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
     7441 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
     7501 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
     7561 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
     7621 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
     7681 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
     7741 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
     7801 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
     7861 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
     7921 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
     7981 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
     8041 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
     8101 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
     8161 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
     8221 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
     8281 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
     8341 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
     8401 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
     8461 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
     8521 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
     8581 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
     8641 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
     8701 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
     8761 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
     8821 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
     8881 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
     8941 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
     9001 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
     9061 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
     9121 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
     9181 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
     9241 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
     9301 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
     9361 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
     9421 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
     9481 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
     9541 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
     9601 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
     9661 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
     9721 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
     9781 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
     9841 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
     9901 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
     9961 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    10021 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    10081 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    10141 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    10201 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    10261 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    10321 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    10381 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    10441 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    10501 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    10561 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    10621 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    10681 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    10741 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    10801 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    10861 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    10921 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    10981 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    11041 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    11101 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    11161 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    11221 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    11281 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    11341 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    11401 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    11461 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    11521 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    11581 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    11641 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    11701 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    11761 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    11821 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    11881 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    11941 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    12001 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    12061 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    12121 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    12181 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    12241 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    12301 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    12361 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatca
//
LOCUS       DACDEA010000071        12232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDEA010000071 DACDEA010000000
VERSION     DACDEA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.072"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1530)
                     /locus_tag="IV136_002169"
     CDS             complement(<1..1530)
                     /locus_tag="IV136_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466139.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ER"
     gene            complement(1517..1735)
                     /locus_tag="IV136_002170"
     CDS             complement(1517..1735)
                     /locus_tag="IV136_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466140.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1728..2693)
                     /locus_tag="IV136_002171"
     CDS             complement(1728..2693)
                     /locus_tag="IV136_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8466141.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2883..3167)
                     /locus_tag="IV136_002172"
     CDS             complement(2883..3167)
                     /locus_tag="IV136_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466142.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3180..3473)
                     /locus_tag="IV136_002173"
     CDS             complement(3180..3473)
                     /locus_tag="IV136_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466143.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3542..4306
                     /locus_tag="IV136_002174"
     CDS             3542..4306
                     /locus_tag="IV136_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466144.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            4357..5502
                     /locus_tag="IV136_002175"
     CDS             4357..5502
                     /locus_tag="IV136_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8466145.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(5587..5892)
                     /locus_tag="IV136_002176"
     CDS             complement(5587..5892)
                     /locus_tag="IV136_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8466146.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="IV136_002177"
     CDS             complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="IV136_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8466147.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6346..6612)
                     /locus_tag="IV136_002178"
     CDS             complement(6346..6612)
                     /locus_tag="IV136_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8466148.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6866..7591)
                     /locus_tag="IV136_002179"
     CDS             complement(6866..7591)
                     /locus_tag="IV136_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8466149.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7957..8027)
                     /locus_tag="IV136_002180"
     tRNA            complement(7957..8027)
                     /locus_tag="IV136_002180"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7993..7995),aa:Gly,seq:ccc)
     gene            8144..8896
                     /locus_tag="IV136_002181"
     CDS             8144..8896
                     /locus_tag="IV136_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8466150.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8912..9391
                     /locus_tag="IV136_002182"
     CDS             8912..9391
                     /locus_tag="IV136_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8466151.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9642..11222)
                     /locus_tag="IV136_002183"
     CDS             complement(9642..11222)
                     /locus_tag="IV136_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8466152.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11215..11958)
                     /locus_tag="IV136_002184"
     CDS             complement(11215..11958)
                     /locus_tag="IV136_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466153.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12134..>12232)
                     /locus_tag="IV136_002185"
     CDS             complement(12134..>12232)
                     /locus_tag="IV136_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8466154.1"
                     /translation="VTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3862 a         2409 c         1938 g         4023 t
ORIGIN      
        1 tctttcacta gccattgtag ctccatctga agctaaacct gataatccac gttcatgggc
       61 atatcccaaa gctcttccag ttttgccagc tttttttgat agagtttcgc cgaaattctt
      121 ttctggtttt cgatcttctc cttctccgct gtctgatcca tttccatttc cgcttccttt
      181 agcacgatta aatccatttg atccttttct aaacagcatt gcgcccatac caaaagcaga
      241 agcaagttgc ccataacctt ctttgagtcc aatatcaaca ccaaagtatc tcatgactgt
      301 tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt ttataaaaat
      361 atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa acatggatag
      421 tccaaatcct tgaaagccta cagtcaaata gcactgtaga atatctgata gaacaacttt
      481 tgaacgctgt cctgtttcta agtcagtagc aaatactaaa actccgagta cacgtttaaa
      541 tgctaactcg agtattgcag taataatcac aaaggcgcta aaaacatagg ctattgctaa
      601 agctatcagc ccaattgaaa tgccccatag attcgcttga tagcgataat atcctgattt
      661 gataccatca acaaaggttg acaaaaaatt atctgaaaat ttagtagcaa caaattcatt
      721 atcagaattt tcaactaatt cataacgcag gttatcagct gttgagttct ctttttccat
      781 tttatcaatt acattatctg tcaatacttg cgtaagatcc gttttccaaa aattttcttt
      841 tgttagattg ttccgatcat tcgttttatc gatcatggaa tattgatccg ttttattgat
      901 ataagctaaa tcagtaaccc cttgttcaat cagcgaccat gccacaccgc catcttgatt
      961 gttaatacta gttgcgtcat tataaagagt ctttgcaaac attattcctg aaagtttttc
     1021 tgttgatcca atcaaagtcg gcattaaaag aattaatact atcgacatta aaacattgat
     1081 tccaacagat tttagatcaa ccgttccttt tccagtcacc attttataac caataatcac
     1141 tagtgaaagt atcattaagc caacaacaat agtgcttaaa actgactgat agactttgtt
     1201 caatccagca ctttccacaa agctaaacaa atctaacact tcaggaatca tattttgaat
     1261 accatctaca attgcaaaca tcgattttat aacagaccac cctatccaca atccaaattg
     1321 actgtaaaaa tttccgttat atttttttaa atacggttga tagacaggat tattcaaaaa
     1381 ttcagtttga ctttccgtat catcgaacca attaaaaatg cttgcatgag caacattacc
     1441 ggtgaaaata acgataaaca ttaaagcaag taagctcaga gctaaccagt ttattttcct
     1501 accagcaatc ttttttttaa ttattttcat tgttacctcc ctcagcccat ttttttaggc
     1561 tctcagaaag cttctgagcc atttcttgat taattatgat atgagttaca caattgtcat
     1621 tttcagctct taaatcgaga accacggtgt taaagcagat actctcgatt aattcgagag
     1681 tatctacatt ttgatcgaga cagcagattt ggtctattag acgattttca atcatataac
     1741 acctctcttg tttgagtaac aaagttatta tatgctctgg aacttacagt atttttgcag
     1801 ttatataaag ttgtaatcca gtaaagattg agattgtgta tctcatgatc aattgcgtta
     1861 aatgaatcaa tagcagattc aatatcaaat tgattgagtt gttctacttt ttctatcact
     1921 tcatttgcag atttttcttc tctatttatc ctgtatgttt tgctcttttc agagtatatt
     1981 gattgaatta tattaacaat atcttcaaca attcttttgt aacaaggatt ggattttagc
     2041 gcattatatc caatttgatt ctttattttt tgtctcataa tttcgacttc catgtcatcc
     2101 gatagattga tagattgatc ttccctaatc tcacctaaac taaccttacc tgacctactc
     2161 tgatctgaac taacctgacc taatctaacc tgggcatgcc catggtcgtc ctttggtaga
     2221 ccaacgggat accatttttc aatcaaatca tttcgaagca tttccgtttg acctgctttt
     2281 aaaatttttt caatactaat ttgttttaat cttgtaaaaa agtctggatt actatttaat
     2341 aatttaaatc gatatataga gggtgtatat cttgtagttc ttaacgtttg attttgcagc
     2401 caatggggaa gaaatactaa taaatcttca ttcagcatat agagaaattc tttagctaaa
     2461 agaattttca cgtcatcatc aggggttcca agcatccgta catatggata tgcttcgaca
     2521 atcccatcat cgtctgcgtt tacattcaga tatacatata ataattttgc tgaatccgac
     2581 atcttcataa aactcgcatt atttaaaaaa gtttttgaca acatacgttt ttctgccata
     2641 aattccacac ttcctcctgt caatgttaaa atgtagtcaa ataaagtttt cacagaactt
     2701 tattattgag tgttcttccg gtctgcaaac tgtaaggagc actctttttt tgtatattca
     2761 ccttttatta aatcaaatag aaaataaatc ttgatttctt ttaaactcaa ctttgcttct
     2821 tcatcatcgt tcaaacaaac acttcctttt tttcatatca aaagcacacc acgcaacttt
     2881 ttttatattt catattcttt gataaatctt gctaactctt cttttctgat aaattgacag
     2941 tctccgatga tactcacctt taaaccacgc tgattaatga atttttttag tgtacttcta
     3001 ctacaactca aatattcaca cgcttctcca agccggaaaa tttctttgct taaagatgtc
     3061 tcaatacgaa cttgatcaac agcttcctta gcaacttgat aaaaatattt acgtaatgat
     3121 tgatcaagat ctggcggtag aataacttgt acactcgata catccatgat gcccactcct
     3181 cattcgtctt ttttcgtctt caaatcacta aaaaaaatat ccacaatatc taaatgaatt
     3241 ccatctttat gaaatagctt tacgattctt tctatttcat aatctttaaa cggcaccctt
     3301 cctttctctt tgttacgata cgttagttcc gatatttcta ataactccgc aagttctttt
     3361 tgtctatatc cacagaacct gcggtatttt ctaactggag agtccaacct taccacctcg
     3421 tcttttttcg tctttaccaa cgatagaata gcatttattt cattgtccgt caatacttac
     3481 ccgtcgtttt tcgtagattc cattccgttt ttgtgttaca ttactgtcgg agggatataa
     3541 tatgaacctt aataagacat cagtaggaaa ccgtattcgt ttagttcgta aagaactcgg
     3601 gctttccatg aaagagttcg gaaaaaagat aactcctacc gccgcagata gtattgtttc
     3661 gcgttgggag cgagggatta gcttacctaa taacgacagg ctaactcaaa ttgctaaaat
     3721 tgctcataaa gatattaact ggttattatg gggaactttt aaagaattta tttcatcatt
     3781 gttagataaa gaaggataca caaatttttt gaatgatttt cctgaaacag tagatactat
     3841 ttataatgat ttaatgccta tttcggaaaa tttacagttt cctgattacg aaaattctat
     3901 tttacgacta ttcaataaga tatacttacc tatattcaat gattactgtc tagacattat
     3961 tgaactggat ttggaggact ttataaataa gagacattcc atctcctctg acaagtttaa
     4021 aaataggttt ttatcctcct ttcatgaagg agtatctaac agcggatata aatacggcga
     4081 tacggattta atttataaat atggaattgc attacttgag aaatttgacg attcttataa
     4141 attaaataat ccatatgagt caatagatgc gtatttttct gccatgactg taaacactga
     4201 agataccgaa aaacttatat ataccatttg taaatcgctt ggcattccat tccaaaaagg
     4261 aagcaatacc gcacagattc tttcaaaaaa ccacaataat ttttaattca ttttttctat
     4321 caaaagcacg ccacgcaact gataggagga aaaacaatgg caacaattaa gcaatatgaa
     4381 aagaaagacg gaaccaaagc ttggctattt cagacctacc ttggaatcag tccattaact
     4441 ggtaaagaag tccgtactac acgtagaaat tttaaaacaa aaaaagaagc tcaaatcgag
     4501 cttaatagac tacttgtaga ctttgaagag aacggactta aaaaggaaac aaacgaaact
     4561 ttcaaagaag tgtatgatct ctggtatgag agctataaaa ctactgtaaa ggaagtcact
     4621 caattaaaaa ttgagattaa gtttaataaa tggattcttc caaaatatgg gcagtttaga
     4681 attaaagaaa ttactacaaa acatgttcag aagatattga atcagtgggc taaaacaaca
     4741 gatcaatata aagtcctaca ttctactgca agccgtattt tcaaatatgc aatgacttta
     4801 gggataatta ctaaaaaccc ctgtgaactt gtaatcatgc ccaatagaaa cgttgaaaaa
     4861 aagaaagata ctgtcaaaat ttattcacgg gatcaactag aaagtctctt ccaatacttg
     4921 gaatcaaaaa gaagcaccta tagagccgac tacgataaaa cgctacttcg ctttctattt
     4981 tttagtggtt gtcgaattag cgaagcttta gctttagatt ggtcagatat tgactttgat
     5041 tcaaacaccg tcactataaa caaaacctta agccaaacaa aatatgggta caaaatttca
     5101 agtccaaaaa cgagaaatag ctatggttct ctatctttag acaataaaac cattaattgg
     5161 ctaaagaaat ggcaaatcaa tcaaaaaaga tatatgttaa cccttggtat tactgatcca
     5221 acaatgattt tttgtggaat ctacaaggaa atcattacta aacacgctat ttattctcgt
     5281 atgataacaa tctgcaagaa ttctggtatt ccatttctag gtaaccatgt aaccagacac
     5341 actcatgcta gtatgctgtt agagtcagga gtctccatga aggaagttca agaacgcctt
     5401 agacactcat caatcaaatt aacaatggat acctatgctc atctaactaa agaaaccaaa
     5461 gaaaaaaccg ttgaaaagct tgcagagcac ctaaatttct agcggaacgt attcaatctc
     5521 gtattcaatt caaaatacaa aaaagctgaa cccttatcta ccaagggttc agctaccatt
     5581 taaagcttat tcttcttctt ccgccatgaa tgtattgaat acttcttcga tcatatccca
     5641 ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt tttcgatgta
     5701 tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagctggat ataaaagaac
     5761 atagttacgt ccaaattctt cttgtccgtc aactgttagc aagatttcgt ataatgtttc
     5821 atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcgtgttcgt gggaatggtt
     5881 atgttctgtc ataagattac ctcttttctt tttattatta aaataattgc ttacctgagc
     5941 cgtctaaata attttgtaaa atcatcacag ctgctaattt atcgatgact ttttttcgtt
     6001 tcgctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttagtc
     6061 gttcatcttg ataatcaacc ggcaaggaga atagttcttt gatcttctct ccatatgaca
     6121 tggaagcttc tgcccttggt ccgatggaat tgttcatatt cttaggtaat ccaactacaa
     6181 aacggtctac ttcgtactgc gccactaatt ccttcaaccg atcaaaacca aactcttctt
     6241 ctgcctcatt gattcgaatg atttctactc cttgagctgt ccagccaaag ggatcgctca
     6301 cagcaacgcc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
     6361 atgatttgag aggtagtctt tgacaattac ttccatgatt tcatcgcgtt cgtgacggcg
     6421 aatcaaattt cgtgcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
     6481 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
     6541 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
     6601 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacac
     6661 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ctgaataaat
     6721 taagaaacac gatttgtctg atttttcctt ttttctgtgg ctttttcaaa agccctcttt
     6781 ccataaagta tacgctatat ttctttgaaa gtatgttttt acacaatttt ttcataaagt
     6841 tcataaaaaa ttcgaattgc tgctactaat ttttcgtttt cgttgcttgc cctgtctcat
     6901 aattgatggt gtagccatct tgaacattgt agataaagac attgaattca atttttttgt
     6961 cttcaatgct ttctgcttcc aactgcacac ctcttgctac cagttcattt ccctcgaaat
     7021 gaggagtgac acgatagcgt acatgatgat ttgtttcctt gatatatgcc gcaatatcat
     7081 tttcgtgggc aagcatgtac ggagcattca acgaacgtgt ccctgtcatt aagtttttaa
     7141 tgttattatt ttgaccagtc aactggaaac caatcaaatg gctgcggttg tataaccatt
     7201 gtccgtcact taatttcttt tgtttccaac ctgtcggatc aatatacaaa ggttctctct
     7261 tttcagtagg aaacaagtct tttcccaaca tcgcattggc ttgtcctact ctgttcaatt
     7321 tgtctatatt tgaatagctt tcccatgtcc ctttggaaag actaagatcc tcttttgtaa
     7381 actccggctg attcccattg atttctattt cttgatgttt tccatcatat tctggcacat
     7441 ccgctacttg agaagcttgc ggtgtggaat caaaaatggt tgtaatttgt tggaagatat
     7501 ctgaatcttt ttgtgtggtg gaaataccga gtgcccctgc aatcacagca gcgacgatcc
     7561 ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa cagttcccta
     7621 aatcttaaca tatgttcccc tataaataaa tactggaaag aaatagaaac agtaaaaaaa
     7681 taaaaccagt ttttagacgg cttaagaata caaaaaaaga gacggattca tcgtctctac
     7741 tcaatcacaa aacactattt acaaggtatt tattatcatt caaaaaattt ttcttaatac
     7801 tcaaaactaa acggcttctg tacgacctct tggtaaacag tgttcaaaag cttgcatctg
     7861 cggtaataag tccaaccaag tcaaaaacat aagaagcttt tttgcttgac tattcttatt
     7921 acttgatgca gggcagcttt ttcacagcct cttttaagcg ggtgacggga atcgaacccg
     7981 cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcgat acaaaaacag
     8041 tatacgcctc tttcactcct tcctcaagtt tttttattct ttacgcttcc gatatgtttt
     8101 gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt
     8161 agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga
     8221 gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt
     8281 ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg
     8341 aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct
     8401 ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc
     8461 aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat
     8521 cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt
     8581 cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt
     8641 tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt
     8701 tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg
     8761 agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc
     8821 tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt
     8881 ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt
     8941 tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat
     9001 ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg
     9061 cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct
     9121 tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct
     9181 agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga
     9241 agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt
     9301 attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat
     9361 gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa
     9421 agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc
     9481 aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca
     9541 tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa
     9601 aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa
     9661 aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca
     9721 ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga
     9781 ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct
     9841 ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg
     9901 aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata
     9961 aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa
    10021 ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc
    10081 aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa
    10141 gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac
    10201 ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg
    10261 attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat
    10321 tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt
    10381 attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt
    10441 tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct
    10501 tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc
    10561 cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca
    10621 aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta
    10681 aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc
    10741 aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct
    10801 cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa
    10861 gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat
    10921 acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa
    10981 cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc
    11041 atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga
    11101 gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg
    11161 agctgtggat taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac
    11221 atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt
    11281 ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca attcaccttt
    11341 cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa
    11401 aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc
    11461 ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa
    11521 cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt
    11581 ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg
    11641 tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa
    11701 aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc
    11761 cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa
    11821 acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc
    11881 ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac
    11941 attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct
    12001 tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta
    12061 ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga
    12121 gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt
    12181 tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt ac
//
LOCUS       DACDEA010000072        12212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDEA010000072 DACDEA010000000
VERSION     DACDEA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.073"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IV136_002186"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IV136_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8466155.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IV136_002187"
     CDS             complement(738..1460)
                     /locus_tag="IV136_002187"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8466156.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IV136_002188"
     CDS             complement(1596..2477)
                     /locus_tag="IV136_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8466157.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV136_002189"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV136_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8466158.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IV136_002190"
     CDS             3717..4196
                     /locus_tag="IV136_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8466159.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IV136_002191"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IV136_002191"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8466160.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IV136_002192"
     CDS             5497..5625
                     /locus_tag="IV136_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8466161.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IV136_002193"
     CDS             5629..6174
                     /locus_tag="IV136_002193"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8466162.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IV136_002194"
     CDS             complement(6629..8320)
                     /locus_tag="IV136_002194"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8466163.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IV136_002195"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IV136_002195"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8466164.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IV136_002196"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IV136_002196"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8466165.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IV136_002197"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IV136_002197"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8466166.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3345 a         2528 c         2205 g         4134 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tg
//
LOCUS       DACDEA010000073        12066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDEA010000073 DACDEA010000000
VERSION     DACDEA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.074"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..662)
                     /locus_tag="IV136_002198"
     CDS             complement(<1..662)
                     /locus_tag="IV136_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8466167.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QL"
     gene            796..1083
                     /locus_tag="IV136_002199"
     CDS             796..1083
                     /locus_tag="IV136_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466168.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1499..3037
                     /locus_tag="IV136_002200"
     CDS             1499..3037
                     /locus_tag="IV136_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8466169.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3034..3999
                     /locus_tag="IV136_002201"
     CDS             3034..3999
                     /locus_tag="IV136_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466170.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3986..5308
                     /locus_tag="IV136_002202"
     CDS             3986..5308
                     /locus_tag="IV136_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8466171.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5371..5475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5549..6292
                     /locus_tag="IV136_002203"
     CDS             5549..6292
                     /locus_tag="IV136_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8466172.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6307..7299
                     /locus_tag="IV136_002204"
     CDS             6307..7299
                     /locus_tag="IV136_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8466173.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7311..8048
                     /locus_tag="IV136_002205"
     CDS             7311..8048
                     /locus_tag="IV136_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466174.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8038..8760
                     /locus_tag="IV136_002206"
     CDS             8038..8760
                     /locus_tag="IV136_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8466175.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8844..9467)
                     /locus_tag="IV136_002207"
     CDS             complement(8844..9467)
                     /locus_tag="IV136_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8466176.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9862..10553
                     /locus_tag="IV136_002208"
                     /pseudo
     CDS             9862..10553
                     /locus_tag="IV136_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            10761..11051
                     /locus_tag="IV136_002209"
     CDS             10761..11051
                     /locus_tag="IV136_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466177.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         4079 a         1999 c         2481 g         3507 t
ORIGIN      
        1 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
       61 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      121 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      181 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      241 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      301 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      361 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      421 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      481 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      541 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      601 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      661 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      721 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      781 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      841 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
      901 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
      961 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
     1021 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
     1081 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
     1141 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
     1201 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
     1261 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
     1321 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
     1381 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
     1441 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
     1501 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
     1561 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
     1621 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
     1681 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
     1741 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
     1801 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
     1861 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
     1921 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
     1981 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
     2041 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
     2101 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
     2161 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
     2221 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
     2281 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
     2341 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
     2401 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
     2461 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
     2521 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
     2581 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
     2641 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
     2701 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
     2761 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
     2821 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
     2881 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga
     2941 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
     3001 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
     3061 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
     3121 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
     3181 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
     3241 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
     3301 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
     3361 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
     3421 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
     3481 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
     3541 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
     3601 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
     3661 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
     3721 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
     3781 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
     3841 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
     3901 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
     3961 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
     4021 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
     4081 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
     4141 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
     4201 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
     4261 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
     4321 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
     4381 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
     4441 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
     4501 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
     4561 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
     4621 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
     4681 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
     4741 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
     4801 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
     4861 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
     4921 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
     4981 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
     5041 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
     5101 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
     5161 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
     5221 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
     5281 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
     5341 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
     5401 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
     5461 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
     5521 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
     5581 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
     5641 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
     5701 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
     5761 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
     5821 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
     5881 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
     5941 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
     6001 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
     6061 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
     6121 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
     6181 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
     6241 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
     6301 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
     6361 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
     6421 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
     6481 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
     6541 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
     6601 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
     6661 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
     6721 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
     6781 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
     6841 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
     6901 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
     6961 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
     7021 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
     7081 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
     7141 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
     7201 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
     7261 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
     7321 aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca
     7381 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
     7441 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
     7501 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
     7561 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
     7621 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
     7681 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
     7741 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
     7801 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
     7861 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
     7921 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
     7981 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
     8041 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
     8101 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
     8161 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
     8221 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
     8281 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
     8341 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
     8401 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
     8461 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
     8521 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
     8581 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
     8641 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
     8701 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
     8761 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
     8821 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc
     8881 gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca
     8941 gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg
     9001 ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt
     9061 gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca
     9121 ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc agcttggact
     9181 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     9241 attgttaatt gttgtcctga gtaaatcagg ttaatatcag aaattgaatt tgattctgca
     9301 atggcattga tcaaggagtt atcaccaacg tatttttgag aaatcgttga tagagtatca
     9361 cctgattgaa cggtataagc ttcgtccgca tgagcagtcg ttcccatgaa gaatgcagcg
     9421 ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg atgtcatgaa aaatctcctt
     9481 tattcgttaa aaagttttat ttgttgtgtt caacgaggaa tactttaaca cgtgaaaata
     9541 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
     9601 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
     9661 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
     9721 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
     9781 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
     9841 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
     9901 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
     9961 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
    10021 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
    10081 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
    10141 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
    10201 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
    10261 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
    10321 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
    10381 gaaaaaacaa atgtagaaat cagttaacag atacacaaaa gaaattagac gaagcaactg
    10441 ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa gatgaagtgg
    10501 aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa taatctttct
    10561 taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactgctg cctctttttt
    10621 ttagtacatg tccttcatct tcgcaggaag tgccaatatg attttaagaa aggtaataga
    10681 caaaaagtca aaaagataaa acagagtttc ttctttacaa agggaatgta tgttcgtata
    10741 atggtattga ggtgaaggtt atggagaaaa aatatggaac agtagataag atccgtactt
    10801 tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa tggggtcaat tgtttgattg
    10861 cttcccatag tttgaacttc ctagctgaag tagacgaagg gatgaagtta gtcgttacag
    10921 gttattataa taatagaaag caatttgttg tgagatccta tcatgtgtta gggaaaccca
    10981 aaattgtggt agaatatgag aaaagtctat acccaagaaa aaaggtccga caagatgaaa
    11041 gaataaacta gaaaacaagc ggctgagaca aaaatgtcct agccgcttgt tttggaaaga
    11101 tggatgcaga cgatgggatt tgaacccaca cgagcatacg ctcacaagaa cctcaatcta
    11161 tatataacta catgtattta agtatttgtg atgaatcact aaactttcca aactcatttg
    11221 agggatattg cttaaagatt gaacagatca attcgaagcc ctattagtat tcgctcaatg
    11281 gggggatcag aggccataaa aagagactag tcattactag tctcaggtag tggaattgta
    11341 ggattttaaa cgttcctgga gagtaccggt atgtaactcg aagaggtgat tatcatagtc
    11401 gtaaaagtaa attgaagaag cctcgccttt cacacgagaa cgtcctggtt cgacagttaa
    11461 acctaacatt tggatttttg agacaaacga atctatatct tgttcgtcaa ttttaaaagc
    11521 aatatgattg tatgtctttg gcagtttatt cgacgaattc tgcattactg caatccatag
    11581 gtccttaact aagaaaaatt tttctggata taatgaaaat ttttttgttg actatcgtag
    11641 atttcttttg catcaaataa ggtatttaac aattaagtat ttaaaagata acttttaagt
    11701 tatctttaag taatgcttaa agctatgtta tactctatta ggaaatataa gaaggagtat
    11761 aaaaatgaac aaacataata gaaaaataac gaataacaaa gaactgatct tgttactaat
    11821 agggatttgg ggtatctttt tactctttaa tatgtatatg ccgactatga gagcggatga
    11881 cgtagtttat gcaagtagat tagatgaact tggctatttg ggtgcttcga tagaacatta
    11941 taagacatgg agttcaagaa ttattataga actattctta atgttctttt ctaaacactt
    12001 tatgctatgg aaattattaa attctacgat tatgttagga attgtagtat tactttgcaa
    12061 gtatgt
//
LOCUS       DACDEA010000074        11995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDEA010000074 DACDEA010000000
VERSION     DACDEA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.075"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            222..2240
                     /locus_tag="IV136_002210"
     CDS             222..2240
                     /locus_tag="IV136_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8466178.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2667..5081
                     /locus_tag="IV136_002211"
     CDS             2667..5081
                     /locus_tag="IV136_002211"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8466179.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5429..6982
                     /locus_tag="IV136_002212"
     CDS             5429..6982
                     /locus_tag="IV136_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8466180.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7363..9147)
                     /locus_tag="IV136_002213"
     CDS             complement(7363..9147)
                     /locus_tag="IV136_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466181.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9162..10883)
                     /locus_tag="IV136_002214"
     CDS             complement(9162..10883)
                     /locus_tag="IV136_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466182.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10983..11744
                     /locus_tag="IV136_002215"
     CDS             10983..11744
                     /locus_tag="IV136_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8466183.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3938 a         2169 c         2429 g         3459 t
ORIGIN      
        1 taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag
       61 aacgtagacg taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa
      121 tatgattaca aaaaacaaca aagctcttaa ataactttta ataaatatta agagaagtta
      181 taattattaa taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa
      241 aagaacggag acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa
      301 tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg
      361 ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca
      421 cagaaattgc tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga
      481 tgatcgcaca agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt
      541 attataatct ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc
      601 ttgaattttt aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt
      661 ttgctgaatc tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt
      721 attactatgc cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt
      781 tgacagggcg ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa
      841 cttacggatt gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta
      901 caggtggcaa taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata
      961 caaactcagg ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg
     1021 gaatctcaaa taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa
     1081 acaatttcat ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt
     1141 cgacgaataa ttccggtaac aataccaact cttcaggaaa tgctggtaca tcaaacggag
     1201 gacaaacaac tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa
     1261 atgatcacgg aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg
     1321 tttatccagg acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata
     1381 ctggctcaaa tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata
     1441 cagcttcagg aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata
     1501 aatacggaat tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct
     1561 acccaggaca acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata
     1621 caggttcgtc aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc
     1681 aaggcactta taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca
     1741 tcacgatgga tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac
     1801 agcaattgat cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg
     1861 cagcgaaacc aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata
     1921 cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca
     1981 cgatggatca gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc
     2041 aagtgattgt gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga
     2101 aaacctatac tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta
     2161 caatggctca actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa
     2221 cgttgattgt agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa
     2281 tatttgttac tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga
     2341 ctgaagtttc agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct
     2401 ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt
     2461 cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag
     2521 agcaatcttt ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa
     2581 ggaaataaaa ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg
     2641 ttttcaatag aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat
     2701 ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa
     2761 aattttacgc actggatatg tttccctatc catccggtca agggctacat gtagggcatc
     2821 ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg
     2881 tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata
     2941 caggaaacga tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca
     3001 attctttagg atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt
     3061 ataaatggac acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag
     3121 aagtagcggt aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg
     3181 atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc
     3241 tgaaaatcac tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag
     3301 aaagcattaa agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat
     3361 ttaaagtggc tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat
     3421 tcggtgcaac ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc
     3481 ctgaacaaaa agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa
     3541 agagaacgga tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc
     3601 cagttaatgg caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca
     3661 caggagccat catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt
     3721 tcggactaga aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag
     3781 aagatggtac gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg
     3841 ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct
     3901 tacgtgactg gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt
     3961 gggaggacgg aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga
     4021 caaaaaacat caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg
     4081 tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac
     4141 aatgggcagg aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac
     4201 tagcagatta tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag
     4261 aacatgcggt acttcattta ctatatgcac gattctggca taaattctta tacgatatcg
     4321 gtgttgttcc tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg
     4381 aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg
     4441 aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt
     4501 cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc
     4561 gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca
     4621 agcttgataa agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc
     4681 atttcaatac agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg
     4741 ctctgcctta tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca
     4801 tgggtgaaga actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc
     4861 caacatacga tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg
     4921 gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag
     4981 cagcattagc caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta
     5041 ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag
     5101 gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt
     5161 ttttgttatt tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca
     5221 aacacgttca atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa
     5281 tttttgtcta ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat
     5341 accaaattca taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat
     5401 gaatgaagta tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt
     5461 atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa
     5521 gcttttaagc ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata
     5581 tcgccatatg agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta
     5641 tcacttttcg tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact
     5701 tatttggctc cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc
     5761 acaacagttt ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa
     5821 gacttcgacg agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc
     5881 acttgatgat ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt
     5941 ccatattttt ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg
     6001 atacctgaca actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg
     6061 aacaatctat tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc
     6121 tcacatgaca atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt
     6181 agctgatcct aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga
     6241 tacagagacg cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc
     6301 gattgattcg caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga
     6361 aggttattgg atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc
     6421 ttctggggat gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc
     6481 tattatcacc caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga
     6541 tggtctattt atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac
     6601 tactatggat ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt
     6661 acaaaatgtc agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt
     6721 ttatacaaac aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta
     6781 tcagacaggg gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa
     6841 tacgttagag acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt
     6901 tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc
     6961 gcaagtagaa caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc
     7021 acaagaaaca aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc
     7081 ggagttgaca agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat
     7141 cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg
     7201 acaagtcttt tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt
     7261 agtattattc tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat
     7321 ggactaatca tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg
     7381 actattataa agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt
     7441 ttcgacgata tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa
     7501 acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat
     7561 caatgcttct gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg
     7621 attcgctaga atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc
     7681 gttttcgcta gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca
     7741 ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc
     7801 gatattatca gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga
     7861 acgacgtagt tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc
     7921 tttattcagt tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt
     7981 tggaccaacg atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa
     8041 tattttatct gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg
     8101 ttctaaaagt cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa
     8161 tatcctatcg attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga
     8221 aattggttca gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac
     8281 accacctgat agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt
     8341 aatgaaacga atggccggat agatagcaca gttcataaat tgagcacgtt taaaagcgtt
     8401 gtattgcttt ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt
     8461 tttgaccact agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc
     8521 atactgattc tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga
     8581 actgccaacg atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat
     8641 cagaccagca gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa
     8701 cacttgagaa agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg
     8761 attgtcaaaa aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct
     8821 caatgtgatc ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct
     8881 gcttaagact gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc
     8941 actgaaagaa agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc
     9001 gattcccatg atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa
     9061 caatccgtaa aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg
     9121 cgtgttcaac gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt
     9181 gcaaactcct gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg
     9241 atttttcctt catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga
     9301 gcaacaatca cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca
     9361 tctgttttat agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg
     9421 atcaacgctc gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct
     9481 tgagcaacaa agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg
     9541 atcttgatcg ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg
     9601 cttaaaattg tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg
     9661 atctgttctt gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta
     9721 gggtcactaa gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca
     9781 acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca
     9841 gagatttcag tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag
     9901 gttggttcag ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt
     9961 tcttctatcc gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg
    10021 atcatcaagt aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt
    10081 tgtaatgctc cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt
    10141 cccatgagca aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt
    10201 tgattagcat agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca
    10261 aaggcacgga tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc
    10321 atttttggct ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc
    10381 catacataaa aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac
    10441 atcatgagaa tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa
    10501 acaatcattt gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa
    10561 ctaaaacgac tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc
    10621 gtttggaatc caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg
    10681 attgttccta gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct
    10741 tcgccacttg ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta
    10801 ccggcgattt ctaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt
    10861 aagaatgttt ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta
    10921 taccttcaag taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa
    10981 aaatgaaaaa aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa
    11041 cgatccgcta ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt
    11101 atcgattgtt tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat
    11161 tagatatacc gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg
    11221 agacacttga tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac
    11281 aaaaagagat aagggagcaa aaaaaacagt tgaaattgat gatcgataca aacgaggaag
    11341 aatttcctgt agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg
    11401 tggaaatcaa aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt
    11461 ccagttcaca gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc
    11521 aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg
    11581 agatgaacca tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag
    11641 gatggaaaac aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata
    11701 tcagaactga atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag
    11761 ataactcgaa aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg
    11821 tttaatgtgg gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat
    11881 gtctttaaaa atggaatggt tgcaattctt agcgatatat acgatgtaat tgtcctctta
    11941 aaaaagtatg gaaaaataat tgtattgggt gttatgtata attcatggtg aactg
//
LOCUS       DACDEA010000075        11921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDEA010000075 DACDEA010000000
VERSION     DACDEA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.076"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(106..312)
                     /locus_tag="IV136_002216"
     CDS             complement(106..312)
                     /locus_tag="IV136_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466184.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(328..942)
                     /locus_tag="IV136_002217"
     CDS             complement(328..942)
                     /locus_tag="IV136_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8466185.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1089..1772)
                     /locus_tag="IV136_002218"
     CDS             complement(1089..1772)
                     /locus_tag="IV136_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8466186.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1881..2339)
                     /locus_tag="IV136_002219"
     CDS             complement(1881..2339)
                     /locus_tag="IV136_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8466187.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2423..4186)
                     /locus_tag="IV136_002220"
     CDS             complement(2423..4186)
                     /locus_tag="IV136_002220"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8466188.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4407..5867)
                     /locus_tag="IV136_002221"
     CDS             complement(4407..5867)
                     /locus_tag="IV136_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466189.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6075..7001)
                     /gene="glsA"
                     /locus_tag="IV136_002222"
     CDS             complement(6075..7001)
                     /gene="glsA"
                     /locus_tag="IV136_002222"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8466190.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7024..8451)
                     /locus_tag="IV136_002223"
     CDS             complement(7024..8451)
                     /locus_tag="IV136_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8466191.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8482..9696)
                     /locus_tag="IV136_002224"
     CDS             complement(8482..9696)
                     /locus_tag="IV136_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8466192.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10037..10279)
                     /locus_tag="IV136_002225"
     CDS             complement(10037..10279)
                     /locus_tag="IV136_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8466193.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10311..10868)
                     /locus_tag="IV136_002226"
     CDS             complement(10311..10868)
                     /locus_tag="IV136_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8466194.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10881..11069)
                     /locus_tag="IV136_002227"
     CDS             complement(10881..11069)
                     /locus_tag="IV136_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8466195.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11082..11648)
                     /gene="amaP"
                     /locus_tag="IV136_002228"
     CDS             complement(11082..11648)
                     /gene="amaP"
                     /locus_tag="IV136_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8466196.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3491 a         2460 c         1949 g         4021 t
ORIGIN      
        1 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttagt
       61 tttatgtgtt tttttatatg ttttttcact tttcggaata aatatttatt tgttaaagta
      121 attaaacaat ccgctaatcg tttgatctgg catttctatt tccttaccat ctaaagtaac
      181 agtagtaacg acaaaatggt cttgcaatgg cggaacttct aagtactgcc cttgcagaaa
      241 atcattcact gtttcatatt cttttttcat ttcttttgaa tctgtcgatg gtttatagct
      301 tatttttaac ataactagtg ctccttttta ttcgggacgt gcaatcgtga aaatttctcc
      361 tattgctgca taatcatttc ctgccaaacg gcttacagca ttcaattttc ttggatcgat
      421 ccttcctgtc ttatcatcat agactgtttc atttaaatga tatttctgta cttctagcaa
      481 aaataaatca gctgttactt tatttttatt ccgtatctcg acatgttcat ataaggatgt
      541 ttcaaaacga acttttgctt ctgtcaatgc tggtactgaa atgattactg aatcagttaa
      601 agtcaagtca gtcaggtaca gttcgctttc ctcattaggc agattggctg ctgttttatt
      661 tacttcttct acgttgtcct catcaaggat atggataaca gcttctcttg tctcaagaat
      721 attctttgct gtgtctttta ctgcatcttg ttttctctgg atcgccaagg ataagatggg
      781 tcgattcgaa ctgacgatat tgaagtaact gaagggtgcg atattcacta tatcttttgc
      841 tgatttagtc gttactactg ctactggtct agggatgact gaaccaatca gcaacttgta
      901 attttcacgt tctgtcaatt ctgaaggact ccgttcaaac atggtttcgc cttctttcta
      961 taaaattcgt taaaaagcag atcttgaaga caaaatgaaa agtaatgctt ttttcatctt
     1021 cttagaatct gctttaaact atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg
     1081 cgtgtttttt agttatttgc tttacttgcg gcttcgacat atcttttagt gatcaattgc
     1141 ggacgggtga taatagatcc aactacgact gaaaaagcac ctaattcata tgctttctct
     1201 acttgttcag gagagtcagt atgtccttcc ataattactg ggatatcgac ttgttctatg
     1261 atttgattca ataaatcaaa atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct
     1321 gctaaagtcg tagacaaaag atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa
     1381 gtcgaaatat ctgccatagc taaccgaccg tgttcgtgaa tcatttggac aagttcagca
     1441 gttgtttttt ctgtcggacg tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc
     1501 gtctccaaaa gttcttccac ttctttttct gtcgcagtaa taaacacttc tgacccatcg
     1561 taatctcttt tgataatccc tattaccggt aaatcaacga ttcgcttgat ttcattgata
     1621 tcttctgtcg tatttgctcg aataccaact gctccgcctt cttttgcagc caaagccatt
     1681 ctccccataa taaaagaact gtgtagcggc tcgttttcca aagcttgaca agaaacgacg
     1741 actccgcctt tgatttgttc cagtgtatcc acaatatctc ctccagtaat gttatataaa
     1801 aatacagctt catataaaaa tgtaaccctt ttctattcag gagacaataa tgagctattt
     1861 ctttcagcag acctttctta ctaaaagaaa attacttcaa attcttcgta tacgaccagc
     1921 ttctcttctg taaattctat gttcaacgct tctttataag catgtttaaa aaactcagca
     1981 tggactttcc gccaataagc taatgtccga tccccttctc cttctttcca agcatgctct
     2041 ttcgtgactt cactaaaagg caagatggtc acatttatat ttttgatgat acaaactggt
     2101 tcattttttc cattcaggac cacgtctaat tgaccaactt ttggtaaagg ctcattctct
     2161 tgtttataca gatcgtaacc acttgatgta ccagtcttta tcttttttac aactagttcg
     2221 ctcaactcgt ctgccatttt ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat
     2281 tgataactag gatatttttc ttggaacttt ttgacatatt cttctacttg ctgatacata
     2341 acatccaccc ctttgctttt tataatgaaa aaaccatcca gatactagta taaacaatta
     2401 tctggatggt gagcataatg aattattgaa tattcaagac acgcagacta tcttcaacga
     2461 tttgttttgg tgttaaatga taacgttcat aaagctgccc tacaggaacg ctatccgtga
     2521 attctttctc tgcaccaaaa tttaatacgt tcattgaaga atcaccataa taacgactga
     2581 tattttcgcc aaatcctcct gacaaactac catcttctaa tgtgatgact aattcgtgtg
     2641 tttcttttaa tttatctaat gtttctacgt cattttctgt gatcgttcct ggattgatca
     2701 acgttgcttc gattcctttt tttctaaaag cttttactgt ttcttttcct aattcaagaa
     2761 aaccgcctaa agctaaaatt gctacttttc ctccattatt tactgtttga taggctggaa
     2821 tactataatc atttgcgata gctgtaccat gttctacacc atgtgtagga attcggattg
     2881 ctacaggctg cgctgtttgt tcgaacgccc aatctagcat agagatcata tcctcttttg
     2941 ttgctggagc aagatatttg atgttcggga tacttgataa gtaaggaatg tcaaatgttc
     3001 cttggtgtgt tcggtcacta gctgagatcg ttccgccacc gactaaaata atcactggat
     3061 tctcgttgat cgctaaatca tgtgataatt gatcatatgc ccgttgcaag aaagtacttg
     3121 attggaagac gactggacgt actccctgag aagccaaagc agaagcgaaa gtgatagagt
     3181 gctgttcagc gatccccacg tcaaaataat gatctggatg tttttcttgg aatgttttca
     3241 gtccaaaggc gcctggtatc gctgcattga ctgcggcaat aggagctgta cttgtttgca
     3301 ttttcttatc caaataatcc atgatgatca tattaaatgt ttctccagaa gttacttgct
     3361 ttgcctcccc ggtggacaga tcaaatggta catgccaatg atatttcatt ttattatcga
     3421 ttgccggctg ataaccgcga cctttttccg tatggacgtg aagtacaatc ggatgatcaa
     3481 tatcttttac ttcttcaaat aaacggatca ccgtttccag atcgtttcca ttatccacgt
     3541 aacgataatc aaatcccatt gctttaaata ggttattgct gcttttaccg ttggtttgac
     3601 gaagctcagc taagttttta tataaactgc catgattctc atcaatagac atctcattat
     3661 cgtttactaa tacgattaga ttggattgta aaacaccacc attgttcaat ccttcaaaag
     3721 ctaagccacc acttagggag ccgtcaccga taaatgctac cacatttcct gattctttct
     3781 tcagatctct cgctttagct aaacctgttg ctaagctgat agatgtagat gtgtgcccca
     3841 ctgtaaaata gtcatgttta ctttcatgtt ggttggaata tccgctcact tcacggtaat
     3901 gtcctttttc aaaaccattt tttcgaccag ttaaaagctt atgaccataa gactgatggg
     3961 aaacatccca aacgatttta tcctttggtg aatcaaatac ataatgaaac gcaatcgtta
     4021 attctactac acctagattc ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga
     4081 ttttttggcg catctcttct gccaactgtt tcatttcctc aacagataat tttttgacat
     4141 ctgcggggct attgattgca tccaatacat gtaatactgt actcataata cattctctcc
     4201 tccgtactat cttcttttaa tgaaaataga tttgttacac tttatctatt ctactactta
     4261 gagttgactc taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt
     4321 agatcttcgc acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg
     4381 tagaaggtct cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc
     4441 tgagcaatat gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc
     4501 ttttcagttt ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc
     4561 gcacatagac attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca
     4621 gcgatgcctt cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa
     4681 ctttctgaaa caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa
     4741 gaaagacttt tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt
     4801 ttctttttcc actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct
     4861 gatagaagat agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca
     4921 aattgtaaaa aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta
     4981 ataatctgta aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca
     5041 acttcgaaat cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac
     5101 aaagaaaaaa tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc
     5161 aatccttgac agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct
     5221 ttcagtccaa ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag
     5281 gacttttcat cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac
     5341 tcgttacaag cttcttctac atacggattt ttattttcat agccttccca attgacataa
     5401 cgtactaaag ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt
     5461 ttcaaataat cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg
     5521 ctcaaagaaa taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa
     5581 taacttaaaa actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga
     5641 gcaataccat gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata
     5701 caatattcta gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc
     5761 cttagagaaa tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac
     5821 agatacactt ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc
     5881 ctccagtttt tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt
     5941 ttatttagaa aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca
     6001 agctcacaat ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc
     6061 tctttcaaga atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac
     6121 aattggatac cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg
     6181 ccgtaacgat ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca
     6241 ccaacatgga cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat
     6301 ttgacgattg tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct
     6361 ttatttgcca gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat
     6421 tgtctgaaat aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc
     6481 atatagtaag caagagagcg attgatatca cctgttcttg attcagaatg atagatttct
     6541 tcattcaatg tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca
     6601 aatttttcat ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta
     6661 acaaaaggat tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag
     6721 gcaaaaccgg ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg
     6781 attgctaata acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg
     6841 tctcctccct caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg
     6901 ggatccactt cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg
     6961 aaattttttc caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat
     7021 tttttatttt tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac
     7081 taagacgata agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc
     7141 gatactagca ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa
     7201 acaagcaacg attactttta caacttttcc gcctggaacc tgataggctc tttcaagact
     7261 gtcatcttta aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta
     7321 gattacaaca gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa
     7381 tgtaaagacg gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg
     7441 aacaccgtgt gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac
     7501 gtgaagtgct tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca
     7561 aacaagaatc gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag
     7621 gaattctaac gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt
     7681 agcgacggtc aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat
     7741 tgctaatgga taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc
     7801 tacgcccata tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa
     7861 atcaggaatc aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata
     7921 atggatccct aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat
     7981 ccgagcaatc aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa
     8041 aataatcaat aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt
     8101 tgcataggat aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc
     8161 aaaccattgg aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca
     8221 agtatagaca ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc
     8281 cacagggata aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga
     8341 ggtcgcgaat gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa
     8401 gccaaagaac gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc
     8461 tcttttctat ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc
     8521 ttcttcttgt tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat
     8581 cactgttgtc agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc
     8641 taagaagtac cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat
     8701 cagaatcgta cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc
     8761 ccattgtttc tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc
     8821 aatcagcatg gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc
     8881 agcaacggat cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat
     8941 gatcagccaa gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac
     9001 tgcttgttca tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag
     9061 cagtcctgta ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg
     9121 aatctttctt tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg
     9181 gatgactgct ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca
     9241 tatccagtgg caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata
     9301 gccttttaaa ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat
     9361 aaacatcagt tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt
     9421 ggggaagctt gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc
     9481 gatatcccgt ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc
     9541 aacggagaga aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc
     9601 gtaaaaaaaa gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa
     9661 tgctgtatct cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt
     9721 acgagtataa aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca
     9781 tttataaaag acagacaatt aataaaagct tgatgtgttg tgctcttttt cgtttttcac
     9841 acaaataaag acaaaaaata aacggtcttc tttttataga aaaccgttta ttttcgtctt
     9901 tttttatttt taaagatatt ttttcataag gaataaagaa tacttcatat aaaatatata
     9961 aaaaagaacc gcagcgagtg tcgctacggt tctcaaattt gttcttcatt tcaacataag
    10021 tttttcttca atttgcttat aatttacgtg caaagaatga aacgactaat actaaaatga
    10081 ttgcaccgat caatgatgga atgatagcca ttcctgcaat tgttggtccc caagttccta
    10141 gtaaagcttg tccaattgct gaacctacta aaccagcaat gatgtttgcg atgatcccca
    10201 ttgatgatcc tttattcgtg attgctcctg caattgctcc aatgataccg ccgacgatta
    10261 atgcccaaat aaatcccatt ttaaattcct cctaactgac ttcttacttc ttattgtaca
    10321 cgtggttcgt tattttcttt gactttttgt gcacctttgc ctacagcaga ttttgcttta
    10381 tctgtttggc gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac tttgtcttgt
    10441 actgtttctt gatctttttg gtattcttct tgtgttttga tgtctgcaac attcacattc
    10501 acttcgataa catctaaatg tgtcatattg tttacttcag tactgatcaa ttctttgatt
    10561 tggttgtaaa tatcttcgat gtctttaccg tattctacga tcacatctag atccacagct
    10621 acttgtttct tacctacttc tgtattgata cctgctgtta cgttatctgt gttgactaat
    10681 ttttcagcca tatttgagaa aaatccgcca tctaatgtca atagaccatc tacatctttc
    10741 aatgcataac cgatgatttt ttggatgact ttgtcttcat aagtcaattc accattcatc
    10801 atgcggtttt ctcctttttc atttgccaaa actgatccat ttgttgtatt catgtttttg
    10861 ttttccatct ttgtttcctc ctatgattta aaaaattgat caactattcc tgactgtttc
    10921 aaataaaaac cgattcctgc gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa
    10981 aatccaatcg ttaaccataa aacagctagc agtagtccaa gacccccaaa gataataggg
    11041 agcttgtatg ctgaaaatag ttctttcatt atttggccct cctattccac tcgagagtgc
    11101 ttgtagtctc ttgtatcttc ttgttcttca taaccgctgt atgtgactgc cactttgacc
    11161 gtttcttttg ttccgagtac tttagttact tcttcttcga tatcttgcat caatacacct
    11221 gttttcccta ctaatgaaga agttcgtttc aagtctccgt tgacatggat atggattcga
    11281 tttttcgttg aacgtatccg tactttaggt gaatgaatga actcttcttc gtgtaaatgg
    11341 gaacgtacca acccttcgat cgcttttttg tcgattgtta gtttgccatc ttcccgtttc
    11401 ataacgaaag ttcctcttga ctttggatag aaaagaacga ctagcaagat gataatagca
    11461 agaacggcaa atactacagc tgcccagaaa atatattgtt gcatgtagta attcgttaaa
    11521 gtaaagaagc gaaatcgttc caattgttca ggcataatat acggcgcatt gatcaacgca
    11581 atcgctagaa atactgaaag tagcagtaaa ctcatgataa caccgattat ttttaacccc
    11641 ttattcattt tccacctcct ataaacttat ttttaccctg taaataactc taccgacaat
    11701 cacagtatag atagcgaaca gttagcatag catcagtaaa taaagcgcta ccaaacattg
    11761 ctggatcata acggcgtttt attgccagtc tgatactgaa ataacaattg cttacttatt
    11821 ctctatttac ttttgattgc tggctatact taaaggatat tattcaagat taaaaattgc
    11881 aaagattatg ctcagttatt attacaagat tctatttatt t
//
LOCUS       DACDEA010000076        11786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDEA010000076 DACDEA010000000
VERSION     DACDEA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.077"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(284..2068)
                     /locus_tag="IV136_002229"
     CDS             complement(284..2068)
                     /locus_tag="IV136_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466197.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="IV136_002230"
     CDS             complement(2068..3819)
                     /locus_tag="IV136_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8466198.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="IV136_002231"
     CDS             complement(3961..4185)
                     /locus_tag="IV136_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8466199.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="IV136_002232"
     CDS             4436..6022
                     /locus_tag="IV136_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8466200.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="IV136_002233"
     CDS             complement(6088..7863)
                     /locus_tag="IV136_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8466201.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="IV136_002234"
     CDS             complement(8074..8976)
                     /locus_tag="IV136_002234"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8466202.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="IV136_002235"
     CDS             9255..11600
                     /locus_tag="IV136_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8466203.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3585 a         2408 c         2195 g         3598 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa ccagagagta taaaatattt tgtgtaaatg
    11761 aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DACDEA010000077        11632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDEA010000077 DACDEA010000000
VERSION     DACDEA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.078"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(126..2201)
                     /locus_tag="IV136_002236"
     CDS             complement(126..2201)
                     /locus_tag="IV136_002236"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8466204.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2203..3120)
                     /gene="glyQ"
                     /locus_tag="IV136_002237"
     CDS             complement(2203..3120)
                     /gene="glyQ"
                     /locus_tag="IV136_002237"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8466205.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3504..4316)
                     /gene="recO"
                     /locus_tag="IV136_002238"
     CDS             complement(3504..4316)
                     /gene="recO"
                     /locus_tag="IV136_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8466206.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4459..5358)
                     /locus_tag="IV136_002239"
     CDS             complement(4459..5358)
                     /locus_tag="IV136_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8466207.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5373..5774)
                     /locus_tag="IV136_002240"
     CDS             complement(5373..5774)
                     /locus_tag="IV136_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8466208.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5752..6228)
                     /gene="ybeY"
                     /locus_tag="IV136_002241"
     CDS             complement(5752..6228)
                     /gene="ybeY"
                     /locus_tag="IV136_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8466209.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6245..8437)
                     /locus_tag="IV136_002242"
     CDS             complement(6245..8437)
                     /locus_tag="IV136_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8466210.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8461..9432)
                     /locus_tag="IV136_002243"
     CDS             complement(8461..9432)
                     /locus_tag="IV136_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8466211.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10137..11516
                     /gene="brnQ"
                     /locus_tag="IV136_002244"
     CDS             10137..11516
                     /gene="brnQ"
                     /locus_tag="IV136_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8466212.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3153 a         2435 c         2124 g         3920 t
ORIGIN      
        1 cacaaaaaat aaaataacaa aaaaaattaa cattgaatta ataatagtta tttatattta
       61 taacaaataa accagagaaa cagcctattt tgtgcctgtt ctctggttta tttacatatt
      121 agaaattatt ttgtgatgat ttgatctaaa cttgctagtg acaaagccat ggcagcaatt
      181 tgacttaatt gcgttaatcg gttcttacgt actgctggtt cttctgccat gaccatcgtc
      241 tgatcaaaat aattttcgat cagtgggcga aggcttacaa gctgatcata attttcgttg
      301 atcgtttggt cagcaaagct tgttttcatt gcctctactg ctttgtgcag ttctttctcc
      361 gcttcgtttt cgaacagttc agggtctact tctgattgtt tttcaagctc cgcttttttc
      421 gctaagttca cgacacgtgt caaagcttcc atcgatggtt tgaagtcttc atctgcaaga
      481 tgctgcttga acaattgtgc tgctgcaaat acttttgtca gatctttttg ttctgcgttc
      541 aaaacagctt cgataacatc atgacggata ttcttcgttg tcaataattg gcgcagacgg
      601 cctttgataa agtcgatcac ctgttgttgt cccgctgaca agccaatgcc aaaacgatcc
      661 gtatctttac tgataacttc actaatcgtt tcttgcagaa cagacaaagg aaaggcccat
      721 tttttcgatt cgatgattcg aacgacacca tatgcttgac ggcgcaaagc atatggatcg
      781 tttgagccac ttgggatcat cccaactgca aagaaagaaa agatactatc taatttatca
      841 gcaatcgcta aaacagaacc tactgtagtc tcaggtagat ctccttcact ggatataggc
      901 atgtaatgtt cacggattgc tgttgctacg gctggtttct cgcctttaag cagagcatat
      961 ttttctccca taatcccttg tagttcaggg aattctccta ccatgttggt tactagatca
     1021 aatttataga tttcagcagc gcgttgcaga tccatcaatt cttcctcaga tagtcctact
     1081 tgttcgccta tgatgcgagc gatgacttgt acccgttgca ttttctcata gatgctaccg
     1141 attttttcat ggaaagtaac agtttttaat ttttcaacgt attggtcgat tgaagctttc
     1201 ttgtcttcat tataaaagaa ttccgcatct tctaaacgag cagtcaatac tttttgattg
     1261 cctcgtacca cattttccaa ttttacccga tcgccattac gagcagaaat aaaatgagga
     1321 agcaacatac cttgatcgtt ccgtacttcg aaataacgtt gatgttcttt catagaagtg
     1381 actagtactt cttcaggaac agaaagatat ttttcctcga agtctccaga aaagacagtt
     1441 ggatattcga ctagattgtt gacttcttct agtaaatctt catctagatc aatgatccag
     1501 tgattttttt ctgctaattc gtttgcttgt gtaacgatca tttcttgtct tttttgcggt
     1561 gaagcaataa caaattgttc atttaatttt tcttcgtatt cttttgcatg cgcaaatgtt
     1621 acatcttcgc ctaagaaacg atgacctctt gaagctctgt ctgttttgac atccaagatc
     1681 tcaaacggga ttatttcgtc atctaacaat gccacgatcc agtgaatagg gcggatatat
     1741 tcaaaatcgt attctgccca atgcatcgtt actgggaaag tgaggctttt aatgacatct
     1801 gctaaacctg tcaatacttc ttttgcttct ttgccgtgag tgtatttcgt tacatagaca
     1861 tattccacgc cttttaattc tctaaaagtg atagcatctg tcgtcagacc ttgtccgcgg
     1921 acaaatcctt cagctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct
     1981 ggtcctttga cttcttcttc gctgtccgct tgtcgttctg gaatatttgt tactttgatt
     2041 gctaaacggc gcggtgtgga aaatgtttcg atcgtatcat aagtcaattg gttttcatct
     2101 aaaaattttg ctgttttttc ttctaattgt ttcatgcttg gtgtgactac atgagcaggc
     2161 atttcttcca gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcac
     2221 gttttctgga tttgctgtgt ttttattcaa taaagggaat cctaattttt cgcgttctgc
     2281 cacaaagatt ttggctacag cacgagccat gttgcggata cgggaaagat aacctgctcg
     2341 ttctgttaca gatactgcac cgcgagcatc taataaattg aatgtatggc tgcatttcaa
     2401 aatataatca tacgctggat gaacaagatt ttcatcgata cagcgtttag cttctttttc
     2461 aaaacggtca aaattgctta gtagtaagtc ttggtcgctt acttcaaaag aatatttgga
     2521 atgttcatat tctggttgtt tgaagatctc gccatatttc acgccatcag cccattctaa
     2581 atcatagaca ctttcaactt cttggatata agaagcaaga cgttctaatc cataagtgat
     2641 ctcagaagta actggtttac aaggtagtcc tccgacttgc tggaagtacg tgaattgtgt
     2701 gatctccatc ccatcaagcc atacttccca gcccacaccg gcacaaccca tcgatgggtt
     2761 ctcccagttg tcttcaacaa aacgaatatc gtgttctaat ggatcaattc ctaaaaggcg
     2821 caagctttct aaataaagct cttggatatt ttcaggtgaa ggtttcatta cgacttgaaa
     2881 ttgatgatgc tgatacaaac ggtttgggtt ttctccataa cgcccgtcag ctggtcggcg
     2941 ggatggctct acataagcag cattccatgg ttccggtccg attgcccgta agaatgtata
     3001 tgggctcatc gtacctgctc ctttttctgt atcataagct tgcatcagca tgcagccgtt
     3061 tgatgaccaa aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat
     3121 ctgttttgcc cctttcaatt tttaagcgtt gctgtcaata agaatacagg aaagaaaacg
     3181 acaaaaagtc cctatgtctg attacagaca tagggacgaa taatttcgcg gttccaccct
     3241 acttccgaat aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat
     3301 cgtttgggtc cggcttccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt
     3361 ggtcaatgta cgtattcatt ttgcagtttt cttgtttgta taatagatcc tctcagtgaa
     3421 cgatctattt aatctacggt taataataga cggaagtccc tgtatttgtc aaatagtttt
     3481 gatttttttg ttgtatcttt catttattct tctttttcag gtatctcttt ttcaggcata
     3541 tttttctctg gcgtctttaa tacgtgtgcc caagacttca tttgatcaat gaactttttg
     3601 ctttttagat gaatacctac atattcatca tacaactggt caatgaggtc acggattgcc
     3661 tgcttcgttt cttctttcaa gtggattcca ctgatcttat cataagaaat agccgaaaac
     3721 attcgaacga aataaacagc gcgaggatct gcatgataac ggtgttcgtc cattggccag
     3781 tgtttctcgc atatcacacc gccatatttg gaagaatagt caaattttcc ttttgtttcc
     3841 ccgcatacga cacagtgcgt ccaaattggc gcgattccga agcgttgcag tatttgaact
     3901 tcaaagatat tcgtgatgat ttctgcatca tctccttggt ccatccgctg cagagcttgt
     3961 tgcgtaaatt ggaataaatg cgggtcatat tgatgatctt cgattgctgc atctactaga
     4021 tttagcaaat acgtaccata accgctcaaa aagatatctt cttggatgtt ccgaaacgca
     4081 tggacgtctt tgacactgct taaaaacgac aaaccttcac ttcgaaaatt accgatatag
     4141 actgcttcgg taaaggggag caaagccgga gctaatggct gattttgccg atggattcct
     4201 ttgatataaa acatcatttt gccagaagat tccgtaaaga ttttgactaa tttatctttt
     4261 tctttgtaat tacggctaga taaaatcaag cctttcgatt ctgcaagctg gctcatattc
     4321 tccctcctaa aaaataaatc tggaaaagca gattctgccg atccagatta tttatgttac
     4381 gatcttccgc tgtttttctc aacgtacgga tttttatcca gtttatttct atacctaacc
     4441 tataagaagt ccagaatatc agtactcgtc ttttcggtag ccgaagtctt gcagatagac
     4501 ttttttgtct cgccagtctt tttggacttt gacccaaagt tctagaaata ctttgtctcc
     4561 taaaagattt tcgatatcct ttcttgcttt cgttcctact tgcttcagca tttttccgcc
     4621 tttgccgata atgattcctt tttggctatc tctttcgacg atgatcgtgg cctggatatg
     4681 gattttatca ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac
     4741 ttcgtctcgt gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc
     4801 aggatgatca gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac
     4861 taatacatcc attaagcgtt cgacattgtt tccttctgta gcagagatcg gaatcacttg
     4921 tgcaaaaggc atctgcgtcg aataatcttc gatgattccc agcaactcgt ctggatggat
     4981 cttatcgatt ttattgataa tcagataaac tggagaatgc acgtttttta gacgttcaat
     5041 aataaaatcg tctccccggc cgcgtttttg atcagcactg atcataaata agactgcatc
     5101 cacttctttc aacgcactgt aagctgcttc taccataaaa tctcctagac gatgctttgg
     5161 tttatggatt cctggtgtat cgataaaaat cagctgagct tctggtgtag tatatactcc
     5221 ttggatttta tttctagttg tctgtgcctt gtcactcata atggcaattt tttgacccac
     5281 gattcggttc aataatgtgg attttcctac attcgggcgg ccgacgattg cgacaaaccc
     5341 tgatttatgt tctggcatat acgttctcct ttttaattaa acaacaaatg atatatttta
     5401 gggacaaata aaatcaaccc aatgatgaca gcaaaacaag cggtcactaa aactgctcca
     5461 gctgccatat ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc
     5521 actacatttt caaaaacagt attgatgatc tctactatcc aaacgagaaa aacagctaaa
     5581 agcagccata accactctat ccgatcaagc tggaaaatca cgcccataaa taaagcaagg
     5641 ataccgaacg caacatgttt cctcatattg cgttcttcct caaaaacggt tttgactcct
     5701 tggacagcaa attccacgga catcaggaaa tgtttgtttt tctctacgtt tttatctttt
     5761 gagtccatag gcatccaaga tctctttctg taaaccaaac atttctttct catcttctgg
     5821 tgtcatatgg tcatacccgt tgatatgcaa gaatccatgt accgctaaaa agccaagttc
     5881 ccgatcatat gaatggccgt aagcttctgc ttgttcagca gcacgttcaa tagagatcat
     5941 gatgtctcct agtacacgag gcaatggatt ctctccatct tcaaaaatca ctgggatttc
     6001 gtcttcgcct tcttcttcca gagcaaaact gatgacgtct gtcggtgcat cttttccacg
     6061 gtaatcacga ttgattactt ggattgccgc attgtccatg aaagtaacag acatttctgt
     6121 ttcctctggc agctttaaat aagatgctgc aaattgcagc aagtcatcga tttctttcat
     6181 tttctcttct gagacttggt ttgtttcatc cataaaggta atatccatta tacatcgctt
     6241 ctttctattc ttcttgttct tcctttatct tctcaacttc tgttttcatt ttaggatatt
     6301 tgatccgaga atggaaggtg ctacttaaac cgctgatcaa tgatttttcc acttttcgga
     6361 tttctttcaa ggtcagtcca ctatcgtcta attgaccatc ggaaatacgc tcttcgataa
     6421 gattatggac aaaactttcg attttttcgc tcgtcggatg atccattgcc cgtactgcag
     6481 cctcacaact gtcagcaata ttgacgatcc ctgcttcacg actttgcggt tttggtccag
     6541 gataacggaa ttgctcttct gttacttccg gattgcgttc tttcgctttg atgtagaaat
     6601 aacgcattag cgtggttcca tgatgctgtt tgcaaatatc gatcaccatt tgcggcatat
     6661 tataatcttc caatattttg gctccatctg tcacatgtcc aaagataata tgtttactgt
     6721 cttctggcaa taagaaattg tgcgggtttt ccgctcctga aggcaagttc tcaacgaaaa
     6781 agctagcatg tttgatcttg ccgatatcat gataatagca agctactcga gttagcaaag
     6841 aacgtccgcc gatttctgct acagcgttag cacttagatt agctaccatc atactatgat
     6901 gataagtacc tggtgcttct tccagcaact gtttcaataa aggatgattt ggattgctga
     6961 gttcatttaa tacaattaca ctgtcatcat tgaccatcaa ttcaatatat gggtgtagac
     7021 cgatcgtcag caagaatgag aagagtgcac cagtgaaccc gcagacgagc attaaccaag
     7081 tcgttgcatc accaaagctc attccttgat agacgatcaa aatgacatcc atcatcacag
     7141 gaaaaataat gacccacatg gcagcagata atccttgttc tgagatccgt ttgcgtctga
     7201 caatcgttgc catgagccca gagaaaacat aagacatcaa gataatcgtt agaaagttcg
     7261 tgccaatcga atcataaaaa atgaaaagtg cggatactgc ttggaaaata ccagccatga
     7321 tccctgctct tcgattcaag aaaaagctta aaacaagtgg tgcaaaagca gcgggataaa
     7381 acagcgggat ataagtagct tgttccgttt ggaataattg gaaaaatttc atcagcacta
     7441 tgctgactga catagctgtc acatagaaaa gcacgtattt tgttctttct cctgcatttt
     7501 ggaattgaag tgaattataa atcagtacgc cgatttgaag caaaacagct aaaaccatgg
     7561 caattagcgg aaaaatcgaa gttgtttgat tagtcagtcc tagtaattcc aattttttca
     7621 tagctgaagc atcgatttga ttaccttcac gaacaatgat ttctccttgg aagatcatca
     7681 ctggttgtac agcttctcgt gcggattgtt tcaattcttc tgtctttttc tcattcaata
     7741 cgtcattcac tacgatacct tgatccacta agtagcgaat catttgttgt tgtgctggtg
     7801 tgacattcaa atattggatt tgctcttctg cttcttgttt aaaagcttcc aattcacttt
     7861 cacgaacttg tttgctcatc tgttcgtcaa tgagcttcag actctcatcc ctcacagtat
     7921 ggatctcagt ttcagtcatt tcaaatatcg tcgaataaaa attgttcggt agtttttgat
     7981 aaaacgcaac attttctgca ttcacatttt caaaattttt cttcagcgcg gcgatccgct
     8041 cttctgtagt aggctttggg atattttcat cttcctttgc cttatcaatc ttttcgtcat
     8101 aagatttgtt gatggcatca ttcgtcttat cgatcaattc aaatagttgc ttgatccgat
     8161 tgttctggtc atctgcaaga tctttttggt aagtgtattc tggagtgact gcttctgctg
     8221 ccagttttct tttttgttcg gtttcttccg tgttctcgat cgttttgttc gcacggatac
     8281 tttcttctgc tacttggcct tctctgaaat cattggcctt ttgtttgaca ctactaaatg
     8341 taacgccgca gataatgata aaaaacaaga acagcaggaa aggcaatgct gcctttccaa
     8401 gtttttcttg tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc
     8461 ttattcttga tggagatctg ccttttcata agctttgatg atttcggcaa ctacagggtg
     8521 ccgaacaacg tcacttgctt caaaattaac aaaatcgatt ttcttgattt gctttaatgt
     8581 tcgttctgca tgaacaagac cacttgttgt ccctcgaggc agatcaatct gactcgtatc
     8641 tccattgaca atcatttttg attgataacc aagacgtgtc aggaacattt tcatctgagc
     8701 aactgtcgtg ttctgtgctt catccaaaat aacgaatgca tcatccaatg tccgcccccg
     8761 catgtaagct aaaggagcaa tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc
     8821 taatccaaaa atttggtata aagcatcata aacaggacgc agataaggat ctactttttc
     8881 ttttagatcc ccgggaagaa agcctagatt ttcccctgct tccacagcag gccgcgtcaa
     8941 aatgattttt tgcacttcac cttttttcaa ggcagcgatt gctaatacta ctgcaagaaa
     9001 agtttttcct gtcccggctg gtcctacgcc aaatacgata tcatgtttgg ctaccgattc
     9061 aacatatttc ttttggccgc tgttttttac ccgtataggt tttccattac ggtctttgac
     9121 gatctcttcc tcatacatct caacaaaata atctaatgtg cctttttgcc ccattttcag
     9181 tgccgtcacg acatctggtg tacttacatg gattcctcgt ttgatcaatt cctgcaatgt
     9241 ccgaatgact gaggcagcca aggaaatctg ggattgttca ccaatgatct gaatgatttc
     9301 tccacgtgta tggatggttg tttctgttgt ttcttcaatc agttttatat gcttgtcgtg
     9361 tgcacctaaa agcatgctga catcatcagc agccgtcaat ctaatctcta aagaactaga
     9421 ctcttccgtc aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata
     9481 ctcagaagaa actatatagc aaataagaaa aggttctaaa agagttgctt aaccataagt
     9541 accaagaaac gttaaaaaat agctgcttcc attctctaaa ggtctcgttt gaataaaaaa
     9601 agctgatcat gtaagctatg cataagatag aggacaagta ttccgtctaa cttagcaagt
     9661 attctctggg tatttttctt gtttcaaaaa aagaaaacaa aatatttgaa atattcttgc
     9721 atttcaaaaa aaacaggtgt attataacgt caacaaatta atatacagca aagcgtcgag
     9781 gaaaacagta ggctattcag cgatttattc ccagtgagtc atgtaagtga gaaatgacaa
     9841 taaatcgaat agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct
     9901 gcctccatta tcaggctagc ggataacttt atcgaaaagc gtccgtgaat gaagaagctt
     9961 ttttaagcga aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa
    10021 gggctttttt tatattttaa ggtggtggtt ttcatggaat ctgatattag tggtatttct
    10081 agtttctgca acgtttattt tgttactcaa aatgataatt attggaggaa aaatttatgg
    10141 aagaaacaaa aacttattca tggaagcagt tattgatggt cagttcactt atcttcggga
    10201 tgttcttcgg gtcaggtaat ctgatctttc ctgtccactt aggtcaaatg gctggatcaa
    10261 actggatcag cgctggtgta ggatttgcca tctcaggcgc tcttttccca ttattagcta
    10321 tcttagctgt agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa
    10381 aatggtatgc tgccttgttc ttgatccttg ttcatttaac gatcggacca ttctttggta
    10441 caccgcggac agcagcaact gcttttgaaa tggctgctaa accctttttg cctgaaaaat
    10501 atgattcatt aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta
    10561 ctgtacatcc aacacgtttg gtcaaatatg tcggaaagta tttaaactcc ttgtttcttg
    10621 cattattagc tatcattttt gtcgtttctt tattccaacc catggggaat ctaaaccaat
    10681 ctgctgcaga ttcttatcag acgaacgctt tgacaaatgg cgttttagaa ggatacaaca
    10741 cggttgatgc agtagcttta cttgctttaa gtgtcacttt tgtccatgca gttcgtgatc
    10801 ttggttttaa agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg
    10861 cgttagaagt catgatttat tttggtctgg tccttctcgg tgcgatgagt ttgaataaga
    10921 tgtctttatc agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta
    10981 acttcggtgc tagcttctta ggggtaatgg tcacattagg tgtcttcact actgctatgg
    11041 gactcgttgt ttcttttgca caagatttcc ataaactgtt ccctaaagtc agttatatga
    11101 catggctgcg gttaacgact tttgtctctt ttgtcgttgc gaatgccgga ttggataata
    11161 tcatccaatg gtctttacca gtattgatgc ttttgtatcc attgtcactt gcgttaatct
    11221 tactttcctt aacagcaaaa ttcttccaaa agagtccatt tgtctatcaa gtaacgatgt
    11281 tcttttctgc cgttccagcc gtattggata tgttggcaaa ttcgcctgca ttagtgagtc
    11341 agcaaactgt cgtagcaagc ttactaggat tttatcatca ttatgttcct tttgctgcat
    11401 taggtcttgg ctgggtcgtc ccaacgttgc ttggttacgc tggcagcttg ctcttttatt
    11461 atgtatatcg cgtatcagga tataaacaag aagcaagtga gttaccagaa gaataaccaa
    11521 atcaaacaca atttaacata agagatatta aacaggaaaa aaaacagcta gaagaaagaa
    11581 taagattctt tcttctagct gttttttagt tcttttttta cgaacacaaa aa
//
LOCUS       DACDEA010000078        11562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDEA010000078 DACDEA010000000
VERSION     DACDEA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.079"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IV136_002245"
     CDS             complement(<1..107)
                     /locus_tag="IV136_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466213.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSR"
     gene            complement(258..2960)
                     /locus_tag="IV136_002246"
     CDS             complement(258..2960)
                     /locus_tag="IV136_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8466214.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2973..4262)
                     /locus_tag="IV136_002247"
     CDS             complement(2973..4262)
                     /locus_tag="IV136_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8466215.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4412..5458
                     /locus_tag="IV136_002248"
     CDS             4412..5458
                     /locus_tag="IV136_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466216.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5460..7613
                     /locus_tag="IV136_002249"
     CDS             5460..7613
                     /locus_tag="IV136_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8466217.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(8086..9537)
                     /locus_tag="IV136_002250"
     CDS             complement(8086..9537)
                     /locus_tag="IV136_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8466218.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(9534..11258)
                     /locus_tag="IV136_002251"
     CDS             complement(9534..11258)
                     /locus_tag="IV136_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8466219.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(11251..>11562)
                     /locus_tag="IV136_002252"
     CDS             complement(11251..>11562)
                     /locus_tag="IV136_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8466220.1"
                     /translation="FLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISV
                     FSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESY
                     E"
BASE COUNT         3240 a         2322 c         2035 g         3965 t
ORIGIN      
        1 tgtcttgaac gattcaagca atgggcagtt ttagcaacag attgattcat tttgaaataa
       61 gactctatta ttacaagttc atccgttgta agatgggtat aggtcatttg tactcactcc
      121 ttataattct ttggtcggaa ctattttgag tgtagcacaa atgacttttt tagttgtcta
      181 gcttaatttt acaatcggcg tttctaaaat ttctccacca tctgaaaccg cacacacaaa
      241 ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa tccgattgtc
      301 cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc ttcaagcaga
      361 ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa atgactagac
      421 atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa agctgtaatt
      481 gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt tccttcatga
      541 tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag
      601 aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc acctagacac
      661 tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact gcgaagtagc
      721 gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa gtttccgact
      781 gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg cggattcgtg
      841 gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa aatactatct
      901 gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg atgatctttc
      961 atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct gattgtcatg
     1021 attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat
     1081 tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg gatttccatt
     1141 tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt tacttcagag
     1201 ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa gatatactca
     1261 tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg tttgtttttg
     1321 cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg gccgttttcg
     1381 atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc ttccccttct
     1441 acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa agtcactttg
     1501 atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata accaaattgg
     1561 acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc tacatgatac
     1621 ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc ttcagcgaat
     1681 ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat tcctgcagta
     1741 tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc
     1801 agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt ttcaaaacga
     1861 ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc gtgaggatga
     1921 ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt tccactaact
     1981 tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg gaccttggta
     2041 ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata ccagccatcc
     2101 gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac atctttttga
     2161 actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg aaacagttca
     2221 tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc aacaccattc
     2281 atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt ttgcttccaa
     2341 aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata ccaattggca
     2401 aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc tgagtattgg
     2461 gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt tacaccacgg
     2521 ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg tcccatattt
     2581 ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg gctttcttcc
     2641 atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc atcttgcaag
     2701 atataaaatg gtccaattct caattttcct tcattgattg cttgttggat tgcctgtctt
     2761 ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc caaatgaaaa
     2821 ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt
     2881 cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct atgagaaatg
     2941 atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat atcaaagtaa
     3001 tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct cgtataattt
     3061 tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca
     3121 agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc ttccatagct
     3181 aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc ttttgcgtac
     3241 tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt ttgcagataa
     3301 tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt gggcacatta
     3361 ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata aatcgtttgc
     3421 cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc tttcttcaat
     3481 ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat ttcttccaca
     3541 taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata acggcaagta
     3601 tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt tgcacctcgc
     3661 ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag
     3721 tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc ttcaataaaa
     3781 gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc caactgtatt
     3841 gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt catttttgta
     3901 tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc atatggatcg
     3961 atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc taattcctca
     4021 tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct taaccacatc
     4081 gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt gactgttgtc
     4141 aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg gatatcccca
     4201 catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc tttataagcc
     4261 actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca tacagccaat
     4321 tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta tggtattatt
     4381 tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc agacaatatt
     4441 taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc aactaccaac
     4501 tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac gtgcattgac
     4561 tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc
     4621 cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc tgtttcttct
     4681 tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc ctgtgatgca
     4741 agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata aaacagcaaa
     4801 agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag aacaacatct
     4861 tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag ataaaaaaat
     4921 ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg cgcttgctgc
     4981 ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta cgcagactca
     5041 tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca aagctggttt
     5101 gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc tttcttatat
     5161 cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta tccagaccat
     5221 gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta tcggatttga
     5281 tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac aagattttga
     5341 acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa aagatcgacc
     5401 atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg agaaataaaa
     5461 tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat ttctctaatg
     5521 ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta
     5581 cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc
     5641 ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt acgccgatca
     5701 atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc ccagaagaaa
     5761 gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc tgttctacat
     5821 tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat gacagcggtt
     5881 tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac acagtggaag
     5941 gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat tttatattga
     6001 gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat agcgactcga
     6061 tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca tcttttatca
     6121 gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa gagacttacc
     6181 ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc acacaccatg
     6241 atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta tttccacaaa
     6301 cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact agtcgatcta
     6361 taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa acggtttatc
     6421 ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt tttttgaaca
     6481 gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc
     6541 ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc cgacgagact
     6601 tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa
     6661 attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct aatcactacc
     6721 acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt agccaagttg
     6781 ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca ttgaactatc
     6841 aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat ggcaagttca
     6901 gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt gcttggcaaa
     6961 gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt ggcagtgaga
     7021 gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac ccaggaggat
     7081 atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga caattagcta
     7141 tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga caacctgaaa
     7201 tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct ccaatgggtt
     7261 accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat agtctgggat
     7321 tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta attgacaagg
     7381 ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct aatcggccgc
     7441 agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa ttatatttta
     7501 cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc atccctcacc
     7561 ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat taacaagagg
     7621 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agatcctcat
     7681 atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc gtttattgag
     7741 aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatggcaa aatagttcct
     7801 catattttgc cattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg
     7861 agaggtcgtg aaaaagctgt ttagtttcga gtattaagaa aaattttgtg aaaatggctt
     7921 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa ctccgtttat
     7981 cgagaggtca tgaaaaagct gccttgcctc aagtaataag aacagcccaa aattagattt
     8041 ctagacaatt ttttataagt tatttcatta cattggcttt ttcttttatg attctccact
     8101 gttttctcgg tactctttag gggatctgcc gacgattttc ttgaataatt tagagaaata
     8161 cgtggtgttg ttgtagccaa tctcacttgc tatctcattg atgttttgat tactgtgcaa
     8221 aagcagttgt ttcgcttttt ccatacgtaa atggttcaaa taagctgaaa aacttttttt
     8281 cgtttctttt ttgaacagct gtcctagata catgacattt aaatgcaggt tttctgacac
     8341 ttctttgagt gtaagtggtt cttgataatg ctggtgcagg attacgatta ctttttccac
     8401 attgtcactg tactttttct gtctctgatg ctttttgatg aatgacagaa gtagctgctg
     8461 aagttcttgc accgtttttg cctgatggat tttttcgatt ccttgtaaat attcctcatc
     8521 ttccagatca atcaattccc gttgaatctc cataaacagt aaaaaactgg aatatcgtat
     8581 atcttccggc atcatggctg ctaactgaaa ctgttcaaaa aaatcagtga tcattttttg
     8641 agcgacttcc aattgtttat tttgcagcac gcgtctgaaa cttgaaaaat cgatggtttg
     8701 atcttttgtt tctgcttgta ctgcgtaaaa aacatgctcg tctttatctc cataaaattg
     8761 atgcaactgt agattgtctt tcgcttgttg gaatgtttca gggattttgt ccaaagaatc
     8821 tgtctcttcc ccgatactga tcagccattc ctgatcagga aagttttctt gaacaaaaca
     8881 acagaagcca tcaacttcat ctgcctccaa caacaacgtg atcaatacac ggtctccgta
     8941 atttcgttga tagtaaaaga tttgttgccg ttcttgcaac catctattga taagagtatc
     9001 atgagttctt gccagttgaa tcaatactat cctatgctgc ttctcactta tttgtcctat
     9061 tatttcttct tcacttattt catccaattc ttcattcaac cattgactga ataacagttc
     9121 ttgatagatt tcttgttgat ttttttgttc attttgctga tctaaccgtg ttttgatttt
     9181 cttgaggctt tcaaccaatt cgaacttatt cacgggtttc atcaaataat tcacggcgcc
     9241 taattgcata cctcctttca aataatcgaa ttcttggtat cctgataaaa tcataaattc
     9301 aaaattatga cgttcttttt gagccgcttc aataaattcc agtccattta tttctggcat
     9361 tgtcacatcc gttactacta aatcaatctg tcgctgttca agtacagcta gtccttcttt
     9421 ggcattctct gctgttgcta ctatttgaaa gcctaaactt tgccaatcaa ttatcttttt
     9481 taacccattc aaaatcatgt attcatcatc taccaataac acttgataca cgctcatccc
     9541 tccttgaatg taatagaaac ggtcagtcca cctgtctcat tattctcaag cgacatataa
     9601 taactgctgc cgaagctggc acgtaaacgt tcattcacgt tttgcagacc gatagaaccg
     9661 tgaagttcca ctttaggatg agacagtttt tcttcaatct gttttagccg tttttcagtc
     9721 atcccttttc cattatcttt gatgatgatc cttacccgtt tgccttcctg caagactttg
     9781 acacttagcg cattatcgaa tcgagtaaaa tcaataccgt gtttaaaata attttcaact
     9841 aatggctgga tcacaaattt aggtacttcg atcttttcca aatcaggatc gatcataaaa
     9901 tgataggcta ctcgattagg ataacgcatt tgatacaaat agacatattt ttcgcaaaag
     9961 tccagttctt cttttaaagt gatcgttttc tcttgattcg tattgtttcg aagcaaagcg
    10021 gaaaacgcat acacaacatc tgctaattct tcacttcctt cgctgattgc atacattcgg
    10081 atatattcta atgtattata taaaaagtgt ggattgatct gtgcttgaag cgcccgcata
    10141 tgtgcgtctt gctgtttgat ttctaatttg tagatgtctt ctacaaactg attgatgcta
    10201 tccaacattt cgttgatacc gattgaaagt tccttcagtt catattccgt gtttgttatg
    10261 tcgatccgcg tctctaaatt ccctgctgct gtttgatcca tggcctgcat gacgatagat
    10321 acttgctgtg aatatcgttt gaatgtttta taaagaaaga taagcagtat attgtctaaa
    10381 ataatgatac caatgatcag cggggcaaga ttttttcccg tttcgatttt tacacttttt
    10441 ttatctagta cagctaatat acgataatca tcagacagtt cttggtgttg aacaaaattg
    10501 tttttttcca gatcgtcaat cggcaattgt gaatttttct tcagactctc tgtgatgatc
    10561 atttcttgtc tttcaaacgt ttctttagct attttattat catggaatgt gtatagtcga
    10621 tttgtcgttc cagaaatcat gtataaagag agtccattga atgacgttag attttctaaa
    10681 atcatatcta gttctttctt ttgaaaacca ataaaaatac ttcctgactc agctcctcct
    10741 tgtgtgatag gcaacttgat ataaaaggcg tctagtaatt ttggtgtccc cttttttatt
    10801 tccccacctt tgttttctct agaagattca taatactccg gtagttgatt caacacgata
    10861 tatatcgcac ttagttcttc ataatttgcg tagagatctt tgatttgatt tgggaaagaa
    10921 actaattcat cggtactgtt ttgctgttcg tatacataca tcagatactg atctatgggt
    10981 tttgttaagt aggtcgtcac attttcgatc ttttcggaat tccctacaag ctggtttttg
    11041 atgatcctac tcaaggttgt tttatcattc agcattctgc ttgtgctttg aacggcatca
    11101 cttgtcatgt tttctgcctg tttttgattt tgttcccaag tatggatact aaaaataacg
    11161 gtagcagtca tcgttacacc gatcatgata accgcgtatt tccgcaacag acgatttagt
    11221 aaggttcctt ttgaaaatag aatccgaaac ttattcataa ctttccaact gtttatctaa
    11281 ttcttcacgc caatgtgttg tcatcgtacc attccaaacc gtcaaaagac caatgacacc
    11341 aaacaggatc aatcctttga attgccaagt cacgcctaaa attataccgc taccaataat
    11401 tatttttaga aaagacgaaa agctggaaaa aaccgaaata aaacttagct ttagcagatt
    11461 aggaacagag atcgtatatt cactatctaa aatcatactg tattgataag tagcaaatag
    11521 gcagagcaag gcaaaaagta aaataaaatc aatagctaaa aa
//
LOCUS       DACDEA010000079        11205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDEA010000079 DACDEA010000000
VERSION     DACDEA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.080"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV136_002253"
     CDS             6..305
                     /locus_tag="IV136_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466221.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV136_002254"
     CDS             378..1739
                     /locus_tag="IV136_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8466222.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV136_002255"
     CDS             1761..2021
                     /locus_tag="IV136_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466223.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV136_002256"
     CDS             2035..2787
                     /locus_tag="IV136_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8466224.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV136_002257"
     CDS             complement(2881..3486)
                     /locus_tag="IV136_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8466225.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV136_002258"
     CDS             complement(3539..4513)
                     /locus_tag="IV136_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8466226.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV136_002259"
     CDS             complement(4891..5670)
                     /locus_tag="IV136_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466227.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV136_002260"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV136_002260"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8466228.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV136_002261"
     CDS             7161..8558
                     /locus_tag="IV136_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8466229.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV136_002262"
     CDS             8639..9079
                     /locus_tag="IV136_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8466230.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV136_002263"
     CDS             9227..9553
                     /locus_tag="IV136_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8466231.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV136_002264"
     CDS             9554..10303
                     /locus_tag="IV136_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466232.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV136_002265"
     CDS             complement(10383..10838)
                     /locus_tag="IV136_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8466233.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3554 a         1960 c         2300 g         3391 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatag
//
LOCUS       DACDEA010000080        11155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDEA010000080 DACDEA010000000
VERSION     DACDEA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.081"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..1384
                     /locus_tag="IV136_002266"
     CDS             14..1384
                     /locus_tag="IV136_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8466234.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
     gene            1371..3005
                     /locus_tag="IV136_002267"
     CDS             1371..3005
                     /locus_tag="IV136_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466235.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3180..4013
                     /gene="msrA"
                     /locus_tag="IV136_002268"
     CDS             3180..4013
                     /gene="msrA"
                     /locus_tag="IV136_002268"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8466236.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4093..5082
                     /locus_tag="IV136_002269"
     CDS             4093..5082
                     /locus_tag="IV136_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8466237.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5371..6069
                     /locus_tag="IV136_002270"
     CDS             5371..6069
                     /locus_tag="IV136_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8466238.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6219..7535
                     /locus_tag="IV136_002271"
     CDS             6219..7535
                     /locus_tag="IV136_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466239.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7552..8967
                     /locus_tag="IV136_002272"
     CDS             7552..8967
                     /locus_tag="IV136_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8466240.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9432..9752
                     /locus_tag="IV136_002273"
     CDS             9432..9752
                     /locus_tag="IV136_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466241.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            9778..10095
                     /locus_tag="IV136_002274"
     CDS             9778..10095
                     /locus_tag="IV136_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8466242.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10124..>11155
                     /locus_tag="IV136_002275"
     CDS             10124..>11155
                     /locus_tag="IV136_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466243.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         3709 a         1767 c         2235 g         3444 t
ORIGIN      
        1 ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga tagatgctgc
       61 ttttttattt agttgtttat atacttttgc aacttatcga acagttgata tctattatcg
      121 atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat attatgctta
      181 tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta taagtctact
      241 tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt gtatgattat
      301 agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg tcgtgaattt
      361 agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc ctatgttgca
      421 gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc tagaagcact
      481 ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat acactatggg
      541 gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa tggagaaaaa
      601 agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta atttatttcc
      661 atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc gtatcttagg
      721 tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa agactatgaa
      781 taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg tcacgtctat
      841 agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata ttgttatcaa
      901 tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg attacgcccc
      961 aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag taggagaatc
     1021 gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag tggcaagcaa
     1081 caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg taaaagtaaa
     1141 cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg atgtaaatgc
     1201 tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca tggctttatg
     1261 gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat ttgcacctaa
     1321 taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga atgaaaataa
     1381 ataaaaaagt agtaatattg agtggattaa ttatagttag tagtatatat tctttcatct
     1441 ttttcaaaaa tacacagagt atttatacca ctggagatct ttcttttcac ttatctcgaa
     1501 tcaaggggtt atcgactatt tttaccagtc cgattaatta tgagacattc aattatactg
     1561 gatatggtgt aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt
     1621 ggatgacaaa aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta
     1681 caacactcgt agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt
     1741 tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg
     1801 caattgccga agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa
     1861 tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc
     1921 tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt
     1981 ttattcgttt ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat
     2041 tatttagtgc ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg
     2101 aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat
     2161 ctaatgtgtt cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg
     2221 gactagttgg tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca
     2281 tagaaaaaaa gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc
     2341 catggtatct tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa
     2401 gcttgcaaat aatattctct tctctgatat taacgatgat atttttcaaa aatagaagat
     2461 acaataagac aagagaacta ttttatttat ttatgactgt tttgttatta atcactttaa
     2521 ctgtttctag taaaaaaaat ttcaatcaaa aaataatttc tcagccggga catattgttg
     2581 taaacaaaga aacagtagag gcgtatacca ctaaaggaat ggattatttt ttatatgatt
     2641 attctcctat cgcttcattg gagaataagg agcatataat aaaacacgaa gcaaagattg
     2701 gcagtcaatg ggtaaacctt ccttataaaa caactggtag ttctatcata tatagagtaa
     2761 ctaatgagaa gagccaagta atcacacttc ctgtatatgc ttatttagga acgaatgtag
     2821 aaataaatca tcaacctgta aaggagcagg tgaatgctaa tggattggta caaataagcg
     2881 gagtcaaagg agttgctgat attaaagtaa gcacaagtta tacagtaacg actattattt
     2941 cttatcttgt tacattaact gcatatttat gtttattttt taaaatagtt ttccataaaa
     3001 ggtaaattaa cttttcattt ataaaataaa ttgattaaaa aggagaatct gtcgataagg
     3061 attctctctt ttttatacga aaaatctgta gggggatgaa gtaatcgctt atttcctcac
     3121 tttatgataa attacagcta aagtcactag cttgtcttct tcggaaagga ggagaatcta
     3181 tggatgagga acaaatattc attcaaacac tttataatct gattttgaat ccgaatacac
     3241 gagattggga acgaaaagta ttgattcaga caaagaatga cataagagaa aatatatccg
     3301 ttaaagaaca gttatcaaaa ctagaagcaa cactacgtcc attagctatc cgaatgaatc
     3361 tgacgcctga tgtaatggac ttttatttat tgattacgga aggatttgat aaggagcaaa
     3421 aatatgactt ttccaagcat gccatgcaag atgctgacta tcaagaacgt gcagtatttg
     3481 cagggggatg tttctggtgt atggtcgagc cgtttgaatc gaaaaaaggg atcctatctg
     3541 ttttgtcagg atacacgggc ggacatgttg aaaaaccaaa ttatgatcaa gtaagtggtg
     3601 gttacacagg tcatgtggaa gctgtggaga tcatttatga cacaagagaa ataagttata
     3661 gcgagttgct gacaatctat tggcaaatta ctgatcccac agatacattt ggacaatttc
     3721 aagatagagg gaagcaatat cgtccagtga ttttttatca agtcgaacga caaaaagaat
     3781 tagcggaaca atcaaaacag aaactagaca gttcaggaac tttccaccaa ccaatcgtca
     3841 caaaaatcga accagccggt acgttttggc cagcagaaaa ctatcatcag caattttaca
     3901 aaaaacaacc gaaacgttat aaaaaaatcc agcaagcacg taatcaattt ctgatctatc
     3961 aacgagtaaa aaacaaatgg caaaaaaata tacgaaaaaa tcattttgat taactccttt
     4021 tctttaaaat gtagtaaact aaaaatagaa aaaagaaagc gttttaaaaa gatttgatga
     4081 ggaggagaaa aattgtatat tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa
     4141 tccggtcatt caggtttgaa attaccgatt ttatctctcg gattatggca aaattttggt
     4201 gattatgatc ctatccataa tcaaagagag attttaagag gcgcattcga catgggaatt
     4261 acccatttcg accttgcgaa taattatgga ggccctgctg gagctgcaga gaaaaacttt
     4321 gggcgtattt ttagagaaga ttttcaagcc taccgtgatg aactgattat ttctagcaaa
     4381 gcaggttatc atatgtggga agggccatat ggggaatggg gctctcgtaa atccatcatt
     4441 tcaagttgtg accaaagtct ccaaagaatg gggctagatt atgttgacat cttctatcat
     4501 catcgcccag accctgatac acctttggaa gaaacagcag aagctttgat gcaacttgta
     4561 agacaaggaa aagcattata tattggtata tccaactata atggagaaga tacaaaaaaa
     4621 atgactgaaa ttttaaaaag aaaggaagca ccgttcatta ttcatcagat gcgctacaat
     4681 atgttttctc gtgcactact agaagacgat ctatctccag tattagaaga agaagggcta
     4741 ggggcaatca cgttcagtcc gcttgctcaa gggttgctga caaatcgtta tctacatggt
     4801 attcctgaag attctagagc acatcgtaag gaaataccat tcctttcaga agaacaagtt
     4861 ggatcaactc tggaaaaaat caaagcactg caaacaatcg ccgtatcacg aggacaatca
     4921 ttagcgcaaa tggcccttgc atggaatttg cgacaaaaat ctgttacaag tgtactggtc
     4981 ggagcaagtc ggttaagtca gttacaagaa agtgttcgaa tgatggataa tttagatttc
     5041 tcaccagaag aagaattgag aatcgaccaa attctagaat agaaacgatg taatcaaact
     5101 ttttatccat atattaaaaa aaacaaaaga tgacatcaag aatcgaatga agatcgtctg
     5161 ttattgaaca tcatattttt tgtcgttcaa aataaaaatg atcgaaggct aggagtgaat
     5221 tttatcctag tcttttttat ggtaaatatc ttcttcgttt gtatgtctat gaacggatta
     5281 atcaattttg tatacaaaaa atcatcagat gattcaaacg tgttataata attacgaaaa
     5341 aatacatcag atgaaaagga ggtatgtggt ttggaagaaa ataaaaccct agtagaagta
     5401 actactgaca agatccttag atatttaaag gaaatgcagt tgcaagtagg agataaactg
     5461 ccgaatgaat atgaattgtc cgattattta gaggtggggc gaagcacggt gcgagaagct
     5521 gtgcgggctc tagcttccag gaatatcctt gaagttcggc aaggagcggg gacatttgtc
     5581 agtgataagc gagggattgc caatgaccca ctcggttttt ctttgattga agatcaggat
     5641 cggatgatac gagacttatt tgaactgaga tatttattgg agcctaagat ggcaggtttg
     5701 gcagcggaat atgcaacgaa gaatcaaatc gtccaaatga aaaaattaac gcaaaagatt
     5761 gaaacttctt ttcgagaagg cacacaagaa catgttcaat tagacatcga ttttcatacg
     5821 atgattgcag aagctagcgg aaacgtagcc ttgctgcata ttatcccaat cattaatgaa
     5881 tcaattgctt tgtttaataa aaattatgat atccagcatc tgaaaatcga gacgatcgat
     5941 attcatacag agatcgttca ggccatcgaa aggcggaatg cacttgctgc gtttgatgca
     6001 atgactgtcc atatggcaat aaatcggaga gaattacaga aaataatagg agaaaaagaa
     6061 actaattgac agttcatctg atgtatttta tgataaatac atcagatgaa tttttgtttt
     6121 atatttggta aaaaagagga gtaaatagtt tctgttcatc gaaatgtaag cgctgactaa
     6181 tagacaaaaa gagatgacgt caaggagaag aagcaggaat gaggggatct ggaatgagtt
     6241 ttatggataa attagccaat gttttaggga acgtggcaac gagaatcaat aatttaaggt
     6301 atatcatggt gataaaaaac gcatttgctg cattgattcc ggtaattatt acgggtgctt
     6361 ttggcacatt gttttcagca atggtctttg actcggaaaa tggcttagca cagataagtt
     6421 tcttgcgttt tttagaagaa ttaaagccta tcgcatctgc catcagctat gtcacactaa
     6481 gtttcttgac catctatgct gtttttctga tagggatcga attagccaag ctgaataaag
     6541 tggatggtgt attccctggt atcatcgcag tgatgtctta tttatctgtt aatccaactg
     6601 tttatgaatt agtgaatgaa aatcagactg ttatagtaga aaacgtattg gctaaacaat
     6661 atacagatac gaaagggtta tttttgggaa tgttggtctc catcctttct attgaacttt
     6721 attgctggtt gaggaaacag aagaaactac agctgagaat gcctgatacc gtgccaacga
     6781 atgtttctgc tagtttttct gctttattcc cgacgattct gactgtatcg ggtatcgcgg
     6841 ctgcgggatt tatcatcaaa gaacttacag gaatgtacct ttatgatatc atttacaatg
     6901 tcgtacaacg cccattagaa ggtgtcgtac aagggttacc gggtattttg ctattgatgt
     6961 ttattgcgca agtcttttgg gtgattggta tccatggaaa tcaaatggtg aagccgatac
     7021 gggaaccgtt gcttttagcg gcaattgctg taaatacgga agcttttgaa gctggaaaag
     7081 aagtaccgaa cattattact atgccatttt gggatatgta tatgagtatg ggcgggtctg
     7141 gagtcacgat cggtttactg attgcagtat tgttagttgg aaaacgggaa gatatgaaac
     7201 aaatcaccaa gttatccctt gctcctggga tcttcaacat caatgaacca gttatttttg
     7261 gtatgccgat catgctgaat ccaattttag ctattccgtt tatcttaacg cctcttatca
     7321 ctggaacgat tggttatttt gcaaccgctt taggatttgc ggcgaaagca gttgtaatgg
     7381 tcccttggcc aatgccgccg atcgtcaatg cttatctggc aacagcagga aatatcggtg
     7441 cagttattac gcaacttgtt tgtattattg tttctatttt gatctatctg ccatttgtaa
     7501 aagtagcaaa tcgtgtggaa aatgatggga tctaaaaaac aggaggaaat catgaaaatt
     7561 actattccaa aaaattttat tcttggtgct gcatcttctg cttggcaaac ggaaggttgg
     7621 aaaggaaaaa aagaaggaca ggattcgttt atagatagct ggtataaaaa tgaacatttt
     7681 gtttggcata atgggtatgg cccagccgta gcaactaatt ttatggaaca ataccagcag
     7741 gatgtggatc tgatgaaatc catccatctg acacattatc ggacttctat caattgggct
     7801 cgttttttta ctgattatga gaactgtata gtagatgaag aatatgctca gcatataagt
     7861 gaaatgatcg actctttgat cgaagcagac gtagaaccga tgatttgttt ggaacattat
     7921 gaagtcccag cttttctttt ggaaaaatat gatggctggt cttcaagaaa agtagttgat
     7981 ttttatgcta agtatgctca aattgccttt gatcgttacg gtgatagggt aaaaaactgg
     8041 tttacattca acgaaccaat cgttccccaa acgcgttgct atttggatgc tgttcgctgg
     8101 ccgcatgaac aaaacacaaa aaagtggatg atgtggaatt atcataaagt tttagcaacg
     8161 gctaaagcag tagagattta ccgcagcgga aattatggtg gaaggattgg ctgtatttta
     8221 aatcctgaaa tggtctatgc tcgatccaat tctccagaag accaaaaagc tgcttatatg
     8281 tatgatttgt tttataatcg agtatttttt gatccgatgg taaaaggata ctacacagaa
     8341 gagttcctgg agttgtgtca aaaacaatcg atttatttcg atcctgcaac agaggattta
     8401 gaaacgattc agcatcatac agtagatttc cttggaatca atcaatatta tcctaaacgc
     8461 gttcgcgccc ctcgttatca atggaatgaa gagacacctt tccatcctga acgatattat
     8521 gaagtctttg atttacctgg gaagaaaatg aataattcca ggggatggga aatctatccg
     8581 aaaatcatgt atgatatagc catgtatcta aaagaaaatt atcataatat cccttggctg
     8641 ataacagaaa acggtatggg aagagaatat gaagaacagt atatggatga gaaaggaatc
     8701 gtgcaagatg attaccgtat cgaatttatc ggacagcatt tgcattactt gatcaaagca
     8761 acagaagaag gcgcaaattg cgaaggctat atgttatggg cattcactga ctgtgtgtca
     8821 ccaatgaatg catttaagaa tcgatatgga ttagttcgaa tcgaattgaa cgatgaacga
     8881 agcagaacat tgaaaaaatc tgcttcgtgg tatcagactt tgattgataa gagagaattg
     8941 gatttgcctg acgaaatcta taaataaata gaagtttttt gtagatgttc attgtattta
     9001 ctggttattt gaaataaaag aggttgtgaa tccagcgttt tgagctaata cccagggtca
     9061 gctgattgaa caccgtttat tgagaggttg tgaaaaagct gtcctgcatc aagtaataag
     9121 aatagcaaag taaaaacaac atcttatgtt tttaacttgg ttggacttat taccgcagat
     9181 gcaagctttt gaataccgtt tatttgaaaa aagttgtgac aagttttgtg tcacaacttt
     9241 ttttgtgcac gttaaaagaa aaaaggtaat aaaaattaat ttgtgtgttt agtgtgcgct
     9301 ttcttttttt agtgtgtatg caacttggta tagaccttaa atgtgatgat atcaactttt
     9361 taaagcgctt ttaaagttgg cacgctactt gcttatatac aagtgtaagt atagaaagga
     9421 acgtgtttaa aatggaaaag aaaacaatca tgcttgtatg ttcagctgga atgagtacaa
     9481 gtctcctagt cacaaaaatg caaaaggcag cagaagcaaa aggaatggaa gcagacatct
     9541 ttgctgtatc tgcttcagat gcggataatc aactagaatc taaaaatgta gacgttttac
     9601 ttttaggacc gcaagtacgc tttatgcaaa atcaatttgc tgaaaagcta gcacctaaag
     9661 gcattccttt agatgtaatc aatatggctg actatggcat gatgaatggt gaaaaagtac
     9721 tcgaccaagc agaaaaacta ataaacaaat aataaaaatt atcggaggat acaataaatg
     9781 gaagatcaaa aaaatctaga agccatcatg ggtctgatca tgtttggcgg aaatgcaaaa
     9841 agcgatgcaa tggaagcgat tgctgctgcg aaaaaaggtg atttcacttt agcagatgaa
     9901 aaaatcaaag atgcagagga atctttagtc caagcacatc actctcaaac agagatgctg
     9961 acacaagaag cacaaggaaa tcacgttgaa gtcacgcttt tgacggtcca cagtcaagat
    10021 catctgatga catctatagc ttttacagat ttagcaaaag agatcattga tctgtatcgt
    10081 cgattgaacg cgtaatctac agtaaagaaa agagggaaga tttatggata aatttgttgc
    10141 atttatggag aaacatttta tacctgtagc atcaaaaatt ggtgcacaac ggcatttagt
    10201 tgcgattcgg gactcattta tggtgagtat gccgttaatg atcttaggag cattagcagt
    10261 aatgatcaat aacttaccga ttcccgggtt ccaagaattg atgaactcaa tttttggtgg
    10321 tgaatcatgg aaaggattcg gggctgctgc ttggaacggt acatttgcca ttctttctgt
    10381 cttgattgct ttcttgcttg cttatcattt agctaacggc taccgaaaag acggtgtttc
    10441 cgcaggtgtg atttctttag gctctttctt cgctttaggc ggagcattag gtatgagctc
    10501 aaacggtttg tttattgcta ttattgttgg gattatttca acagaaatct ttgtacgttt
    10561 aagcggaaat gaaaaattga tcatcaaaat gcctgatggt gtgccaccgg cagttgcaaa
    10621 agcatttgct tcattactac cagcaatgat cactatctct gcgtttgctt tagttgctgc
    10681 aatttttgct gcattcggtg ttgatgatat cgtaggttct ttctatacag tggtccaaga
    10741 accatttatg ggacttgcaa actcttatcc atctgcctta ctgcttgcat ttatcacacc
    10801 attcttatgg ttctttggtt tacacggagc gaatatggtc gatcctttga tgcaaacaat
    10861 caatgcgcca gccattgaag caaacgttaa tgcaatcgca gctggacata gcgcaccgtt
    10921 tatcgtgaac aaacctttct ttgacagctt tgtcaactta ggaggaacag gtgcaacttt
    10981 aggtttactg ttagctattt atttagtagg acgtaaaaac aaaccatata tggttgtaac
    11041 aaacttgtcg attgcgccag gtgtcttcaa tattaatgaa ccaacgatgt ttgggttacc
    11101 tatcgtgttg aacccgatca tgttcattcc atttattttg acaccaatgg tctta
//
LOCUS       DACDEA010000081        11107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDEA010000081 DACDEA010000000
VERSION     DACDEA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.082"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(519..884)
                     /locus_tag="IV136_002276"
     CDS             complement(519..884)
                     /locus_tag="IV136_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466244.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(960..1205)
                     /locus_tag="IV136_002277"
     CDS             complement(960..1205)
                     /locus_tag="IV136_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466245.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1275..1529)
                     /locus_tag="IV136_002278"
     CDS             complement(1275..1529)
                     /locus_tag="IV136_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466246.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1693..2016)
                     /locus_tag="IV136_002279"
     CDS             complement(1693..2016)
                     /locus_tag="IV136_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466247.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2203..2276
                     /locus_tag="IV136_002280"
     tRNA            2203..2276
                     /locus_tag="IV136_002280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2237..2239,aa:Ile,seq:cat)
     gene            complement(2661..3158)
                     /locus_tag="IV136_002281"
     CDS             complement(2661..3158)
                     /locus_tag="IV136_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8466248.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3198..4007)
                     /locus_tag="IV136_002282"
     CDS             complement(3198..4007)
                     /locus_tag="IV136_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8466249.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4341..5198
                     /locus_tag="IV136_002283"
     CDS             4341..5198
                     /locus_tag="IV136_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8466250.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5191..6093
                     /locus_tag="IV136_002284"
     CDS             5191..6093
                     /locus_tag="IV136_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8466251.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6126..6476
                     /locus_tag="IV136_002285"
     CDS             6126..6476
                     /locus_tag="IV136_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8466252.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6510..7448
                     /locus_tag="IV136_002286"
     CDS             6510..7448
                     /locus_tag="IV136_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8466253.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7526..8419
                     /locus_tag="IV136_002287"
     CDS             7526..8419
                     /locus_tag="IV136_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8466254.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8457..9020
                     /gene="amaP"
                     /locus_tag="IV136_002288"
     CDS             8457..9020
                     /gene="amaP"
                     /locus_tag="IV136_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8466255.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9034..9222
                     /locus_tag="IV136_002289"
     CDS             9034..9222
                     /locus_tag="IV136_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8466256.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9235..10137
                     /locus_tag="IV136_002290"
     CDS             9235..10137
                     /locus_tag="IV136_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8466257.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10169..10411
                     /locus_tag="IV136_002291"
     CDS             10169..10411
                     /locus_tag="IV136_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8466258.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10667..11038)
                     /locus_tag="IV136_002292"
     CDS             complement(10667..11038)
                     /locus_tag="IV136_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466259.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3922 a         1915 c         2117 g         3153 t
ORIGIN      
        1 tctattttca gggggggaac cttatgataa aaaaactatt tttactatca atattaacgc
       61 tagcaatggc aggttgttct gactctggct tagcaactga actgatcaca aaatcaactg
      121 aagccacgac agaggagtta aaatgtggaa acgatcacag tgactacaga ggcttaaatc
      181 gacgatgcat gggaatatta ggcagaagca attgtaagca tcgatgtagt aatcgaagaa
      241 ggtgtagaag aagcaacaga aaaatcaaaa gaaagatcaa aagaagcaat ctgtgctgtg
      301 catcgtatac agacgtcgaa gatgcggtac ttttaaacca cgtcaggaga tgttcattta
      361 tgtctaggga agttggcgat gaaccaaaac tagagtacgc ttttagtttt gcacgtgagg
      421 atgaagataa ccctaaaact tacgacattt tagcttgatc taagatggtt aaagcttttt
      481 aatgaagtaa gaggagatag aaggcttttc tagttatatt aggtaagata aattcctttt
      541 tgctccaaat ttgctaaaca ttctgctaga taaacttcat cgtgttccgg ataaatcggc
      601 acgttgcgct ctacagtagg taaatcaaaa aatgcttcgc atgatttgcc acgatacata
      661 ggatttttcc actctggtgc tgggctgtaa ccacgttttt ccatttcatc catgacaagc
      721 tgatgatatt ggaataatct ataaggagaa tagtgaaaaa cataatccac agtagcatgt
      781 ttctttcccc agccatttcc tcttaatgca cagcattccc gatgttgtcc taaaagctgt
      841 tgacgaggaa gctttgaaat caatttttca tgccataatc gcatagaaat gtctccttta
      901 tatatagatt gttaacctta gacgttaaat gaaaaaacca gcaccaaggc tggaaaaaat
      961 cacttcagtt tgcttgctgt ttctgagcga ccaattaggt aatcaacgga aatatcgtaa
     1021 aagtcagcga tatagattaa ttcaatgatg cttggaattt cttctccttc ttcgtactta
     1081 cgatatttga ataaaggaat acttaacgcg ctagaaacag ctacttgaga taaagatcgg
     1141 ccagttctta agaaattcag acgacaacgt aaagtatcca gtaaaatagc ttcttcgata
     1201 ctcatttaat cacctttcat tatagtagaa tagttataat gactatagaa agctacacat
     1261 gcaatcaata gtactcaatg ttttttattt aaacgtatca aataaagaat gacgatcaat
     1321 aaagacaaaa ccagtcctga taattcttct ataaaaacac tatcgggttc aaagtagttg
     1381 taattaaaaa ctttccacat aaagtagata gccggaagag tcaaaatagc taaataaaac
     1441 cgttctttgt tccaattcat aagataaaca atacttgcga ccagtgcgac cgcaggcacc
     1501 caaaacaaaa ctctcaccaa cgtaatcatg taacggactc ctcaacgatt catataataa
     1561 aagataacat gttattgcat acatcacaaa tgaaatgcta ggaaaataaa agaagaatag
     1621 atcaatgaaa aatgaaaaag cagaacagtt ttctgctgat aagcaggaac tgttccgctg
     1681 aatgtgtctt atctatcgag aaaaaatcaa tggattgata tctaagtaat tcaatacata
     1741 taaaaagagg acgatcgcat tcactaaaaa ggaaagaatg atgatcccat agcgaatgga
     1801 aacatattta acaaatactt ttgtttgaat acgggcatat ctttttcctt tatagctgag
     1861 catcatgaca agaagcaaaa gggtaatcac accttgaaaa atcattccaa taaacagaat
     1921 tcctgaggtt tgataattta attggagaaa ataagctgct gccgcaacta aatcgaataa
     1981 aatcgcaata aatggcaaaa taatcactcc taacattgag aataaataaa agaataacat
     2041 tttttctaaa ataagcaaat aaaaattaaa gtaacaaaat agttatgaca caagtaaaag
     2101 aaataagaat aacaatatta ttttacttat attttgaaac ttgtgagagt ttgtcattcg
     2161 tgctatagtt gaaaagtaac aaaaataaat tttatttatg atgtaccttt agctcagttg
     2221 gttagagcag acggctcata accgtccggt cgtaggttcg agtcctacaa ggtacataag
     2281 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct
     2341 tatgttttta cttggctgtt cttattactg cagatgcaag cttttgaata ccgtttaagg
     2401 aagaggttgt gaaatcagcg ttttgacctg agtattagct taacaaacgg aaaaatagtt
     2461 cctcatattt tgccgtttgt taagctaata ccgagggtca gctgattgaa caccatttaa
     2521 gaaagaggtc gtgagcctgc cgtttcactc tgtgtcataa aaaagccaat ctactttgat
     2581 agaaaaagca cgtaggacta aaacagttct gcgtgctttt tttattgtgt gttaaatatc
     2641 gtaaatgaag atagatattt ttatgattcg acgatagaga cgttgaaatc tttcaacagc
     2701 tctaaagcgg attctccgtg ttttctactg gagactaaca ttggatcaac aaaaaagtct
     2761 gcttcaggaa aagcactctg caggcctatc agattagaga gtacacacaa gtttgtttca
     2821 atcccgacta cttcgatctc tttttgttct tctttgcttt caaataaagt ttcctggatt
     2881 tcagctaaag tttcaggtgg aatcgtaaag taatactttt taatcactcg ttcattttct
     2941 tgtggagaaa gaagagggat caattgcaaa tcttctcgtt cttcttcttt gtccttcagt
     3001 tcaactggta tatcccgtgt gaagagaata tattcattat tttggcgtgc tttagctaat
     3061 ctttgagcaa tacgttctgc caattgatct gactctggaa tataaaattc gctgtttgga
     3121 tcaagaatat gattttgcat atcaataata actaacattt tctttcctcc tacattcata
     3181 tcgtcatttt attcttttta atagaagttt tttaaaatat aagcaacacc agcttcttca
     3241 ttcgtcagtg ttacttggtt tgcaatttct tttacttgtc gctgtgcatt ccccatcgca
     3301 atactcaacc cgctgtaatt caacatagca atgtcgtttt cttgatcgcc aaaagccatg
     3361 atgtcactcg attcgattcc aaggtaatcc gccagaaaaa caagtgaatg agctttagtg
     3421 atacctgtag gcgtgatttc taagaaatgc ggatcagaga atactgattg gataccatct
     3481 gcttgcagat ctctttgtag cttctgtagt ttttcttcac tcttatctgt taatgctaat
     3541 ttaagatagc taagagaagg atcttccaac atatttaaaa tttgatcaaa agaatggttc
     3601 tgtaaattga aatgttcata ataagggtca tctttgacta gatttgattc atcgaatgtc
     3661 agctgatcat cttgggtttc tcggctagca atgatggata ttttcgcttc ttgagcatag
     3721 aagaaggctt ttctcagtgt atcgctatct atttttgaac gtctgagaac tttcgccatt
     3781 tttccatcct tcatttcagc aatcagtgaa ccattggaag aaatcaaata gccaccgatt
     3841 cctaattcgg aaaaaatatc tttagcagaa atgctgttcc ttccggtagc gacggttacg
     3901 attccgccat tttcatcaaa tttccgaagt gcttggatat tttcttctgg tatctttcca
     3961 tttcgatcaa gcaaggtacc gtctaaatca atcgctgcta gtttcatttt atcttcctac
     4021 tttcttttta catatctatt ttcctgttac cttaagatta aatgagaatt taaaaaaatg
     4081 aaactaactt gctttgtcat tatgaatttt ttcaaaaaac attcttaaga aaaaggtggc
     4141 tatatatagt agtttttggt caataccttt gaataaaaag ccatgaaaaa cgaatagtta
     4201 gcgcttaccg atgcgttttg tgataaaaaa attttaaaat attcaatgaa aacaaagcat
     4261 atattttgct ttaaaaccaa atgaggaata ttctagatgt agaacaggtt agaaaaagat
     4321 tagaaaaagg agtgattttg atggagaaga gatacggcgt agttcctgtg gtaactgctt
     4381 cagacgaaaa ttatgctcct tacttgagcg tgatgatcgc aactgcatta gaaaattgca
     4441 ataaaacaag acgcataaaa ttttatgtga ttgacgatgg tctatcagaa tatagtaaag
     4501 aaggattgga agaaacggta aataaatatt caagtaatgc aagtatccaa ttcctgacag
     4561 tggaaaaaga tatctacgaa gacttcctag tcagtgacca tatcacaact accgcttact
     4621 taagaatttc tttaccaaac ttattagcta aagaggatta taagaaagtc ctttacttag
     4681 attcagatgt gttagtacta gacgatatcg taaaactata tgatgagccg ttaaatggaa
     4741 aaacaatcgg cgctattatc gatccaggcc aagtgaaagc tttggaaaga ttaggaatcg
     4801 attccgatga cttgtacttc aattcaggag tgatggtgat tgacattgat caatggaata
     4861 aaaaagaaat tacagaaaaa actatccatt acctcagtga aaatggcgat cgaatcatct
     4921 atcacgacca agatgctttg aatgctgtac tttatgaaga ttgggaacaa cttcatccaa
     4981 aatggaacat gcagacttcg ctgatttttg aacgtcatcc agcaccaaat gaaaaatatg
     5041 aacgtcagta taaagaagga aatgaaaaac cttcgatcgt tcactttaca ggacatgata
     5101 aaccttggaa tacgttgaaa gatcatccgt ataccaattt atacttaaaa aaattagcac
     5161 acagtactct aacgaaagta ggcgagatca atgaataaaa aagaaatagc agtagtcgca
     5221 agttgtaaca caaaattcgt accacattta gctgcattat ttgtctcagt attagataat
     5281 tgcaacccgt ctaaatttgt tcggttctat gtgattgacg atgatatcga ttttgaaagt
     5341 aaacagcttt tacgcttctc agtgaaaaac gcacgaatga actcagatgt ggaattcttg
     5401 aaaatcaata aagaattctt tacaaatgtg gtaatcagcg atcgtattcc agaaacagct
     5461 tattatcgta ttgcgattcc ggaattgttc cgcggcacag aagttgaacg tatcttatat
     5521 atggattgcg acatgatcgc tttacaagat atctctaaat tatggcgtct tgacttcggt
     5581 gattcaatcg tagcagctgt tgaagacgct gggttccatc aacgactgga aaaaatgaaa
     5641 atttcagcta aatcaatgcg ttatttcaac tcaggtttga tgctgatcaa tgtgaaaaaa
     5701 tggttagatg aaaatattac gcaaaaagta ttagatttca tcgaacataa tccagaaaaa
     5761 ttacgattcc atgaccaaga tgcattaaat gctatcttac atgaccgttg gttaccactt
     5821 catccgcgtt ggaatgcaca aggctatatc atggcaaaag caaaaaaaca tccaacagca
     5881 gcaggtgaac gtgaatacga agaaactcgc aacaatcctt acattatcca tttttcagga
     5941 catgtgaaac cgtggagcaa ggattttgaa gggccaacga agaaatatta cgaaaaatat
     6001 gcagggatga cggctttccg ttgtgtagct aaattcccta aatatccaaa atatgcaaaa
     6061 gttcaaccaa aaacagatgt agttgttaaa taatagaaga caacaactga agagggggag
     6121 taggaatgga aattacaatg tctaaaaatg cagtagagac gttaattgaa aaggttgggg
     6181 aaaatacaaa aatcgcactc gcgcttatca atgattccga tccatttcta agggacaaag
     6241 gtgcttttgc aaaaggcagc ttttttcaaa tcattccttt tgtttcggaa tttggagaat
     6301 atgcgactaa aatcgagcat ccattattgg acatctatac atctaaatta gagcaaaatt
     6361 atttcggtaa acgattgaac atggacttca acaaacagct tgacagcttt tcattagaaa
     6421 atgaaatcgc agtactcgat tataatatca aattaaagaa ctgcttcttc agctaatagt
     6481 ttcgagaaag tccaaaaagg aggtatacat tgagatacta ctttgtggat gaagaaaaga
     6541 atgtaataga aacagatgaa aaaaaatata attggctcgt acttgatagt gcaaagcccg
     6601 aagagattga gcaagcaatc gaagaattcg agcttcctga tgatattttc gtaggaaccg
     6661 aatatccgga agaagtttct cggatggagc gcctttatga gacgaaacta gagcatccat
     6721 tttctttggt agtgattaat ctagatcagt cagataatcg tatcgaaaaa aaattaacgc
     6781 caatatcttt tgtactttcc gataatctct tgattacttg tatcgataga aaaaccgaat
     6841 ttgtggatcg gctgctgaca aagcatggaa atcatttaaa ctcgtttgaa aagatcatca
     6901 cgtatacatt attagatatt tacacccatt acgtgaaaga actaagagag atgaaaaagc
     6961 gaattgacgc tttggataaa gcagctcgta aaacaacgga aaatgaagaa ctgtataaac
     7021 aggcagatct tgaaagagac atcgtatata tcgatcatac cttaagagat caacgggaaa
     7081 caatggatca attatggaat tctgaaactt tcatcaaacg tttagacgat gaacagctac
     7141 tctatgatgt ccatttgaga cagcgacaaa cagaaaaaat gatcgagatc tatcgagatt
     7201 tattagagag tattggtgat ctattcaacg ggatgatgga caataatcta aatcatatca
     7261 tgaaatatct agattcagct gcattaatca tttccgttcc ggcgttgttc gcagggattt
     7321 ggggaatgaa tacaggagga ttaccgggta aaagttctgc tctaggcttt atactcgtag
     7381 tctttggctc tttgatagca gcagtagttt taggttacca tttataccgg aaagattata
     7441 caaattaaag aatctattta aaacaggtat caaacaaaga aatttgtttt ggcaatacaa
     7501 tttaaaaact aggaggaata cagcaatgac agaaccaaaa ttaatcgacc cgcgaaactt
     7561 gtaccatacg gagaaatttc ctaaacaaga tcaggataca ccagctttac agcagaatat
     7621 gattcctgtt cctgattgtg gagaagaatc ctatgaaggc aataatcaac tagaaaaccg
     7681 ccgtgtacta atcactggtg gagattcagg gatcggtcgt gcagcagcga ttgcatttgc
     7741 tagagaaggg gcagatatcg ctcttcaatt cttcccagga gaagaaaaag atgcagagga
     7801 agtagcaaaa tacgtgaaag ttgcaggacg taagattgtc ttactacctt ttgatcttcg
     7861 tgacgaacaa gcacctaaag aaatcataga taaagccgtg agtgaacttg gcgggctaga
     7921 tacgatggta ctaaacgcag gacagcaaat ttcttgtcag tctattgctg agttaccact
     7981 tgagcaagtc aaagacacat ttatgataaa catcatcgct atgtttgctt tagtgaaaga
     8041 agctgttccg catatcccag caggaggagc aatcgtaaca acaacttctg ttcaagcatt
     8101 caatccaagc gaacatttat tagattacgc agcaacaaaa gcttcaatcg ctaactttac
     8161 gatcggttta gcaaaacagc ttgcacctaa aggaatccgt gtaaatggtg tagcacctgg
     8221 tccaatctgg acaccattac aactagacga aggacaacct tctgattcga tccctgaatt
     8281 tggtcagcaa agtttactag aacgtgcagg tcagccagga gaattagcac cagtttatgt
     8341 tttcttagca tcgaataaag caagttatgt tacagcccaa atttacggag taactggtgg
     8401 agaagcgatc aacttataag aagttgatcg tttcacccct atgaggaggt gcgaaaatga
     8461 ataaaggtgt aaaaacgatc ggagtgatcg gaacattact tttgctatct gtgcttttgt
     8521 ttgcgatact attaagtgca acctatccat tgccttttca gctagagaag tttcgattct
     8581 tttccatcac taattcatac gtgcaacaat atgtattttg gcttgccgtt gcatttgcta
     8641 ttttgacaat tatcacgtta cttgttttcc tgttttatcc aaaaacaatc ggaaactttc
     8701 tactgaaaca tggtgatggg gagttgacgt tggataaaaa agcaattgaa ggaatggttc
     8761 gttctcactt gcatgaagat gaattcatcc attcaccaaa agtaaatgtc aaagcaacga
     8821 aaaacaagat cgccgtcaat gtaaaagggg agttgaaacg aacttcagca ctcattggta
     8881 aaacaggaac attgatgaaa gaaatcgaaa atgaagtaca acacatattg ggaacaaaag
     8941 agccagtgaa agtatcggtg aaatacacag gctttgaaga accagctaaa cgaagcaatg
     9001 accgcaaacg agtagaatag ggggggacca aaaatgaaag aactattttc gacatacaag
     9061 ctccctatca tctttggggt acttggattg atattagccg ttttatggat caccgtgggc
     9121 tttttcaaga ccttattgat cttgatcatg gcagcgatcg gcgtcgctat cggtttttat
     9181 ctgaaacaaa cgaaaatctt agatcactat tttaaatctt aggaggaaac atccatgaat
     9241 aacaataaca aaaataataa caataaaatg aacaaccaaa aaccagtgtc tgcacatcca
     9301 gacccaacga caccaaacca atcaaatggt gtgaacgatg aaaagaacaa aacagcacca
     9361 gtatctgcac atccagatcc agtagtccct aacgttcaac aaggagtaga agagaagaaa
     9421 catgatgcag aaaagcatgc acctcatcat ccaggaactc cagaatctaa agttccagaa
     9481 caacatgatg caatcgatcc ttctaaacct gtggaagagc aaaaacatcc ttctgaccat
     9541 gcgaaagcac aagaaaaact tagttctgac cacacaaacg gacaaggaaa acttggttct
     9601 gatcatacaa ataaccaagg aaagcatgat tctgaacagg aagattcaat gcgcggtgag
     9661 ctgaattatg aagataaagt tgtgcaaaaa atcatcggaa tcgcaattga acaagtagac
     9721 ggtttattat ctgcaaacgg tggtttcttc tctaatgtgg caggaaaact tgtgaataca
     9781 gataatgtaa cggctgggat cgaaacagaa gtcggtaaaa aacaagtagc agttgatatg
     9841 gatgtcattg tggaatatgg caaagatatc gaaaaaatct ttgaagaaat gcaagaagta
     9901 atcggcaaag aagtaaaaaa tatgacacat ttagaagtga tcgaagtcaa cgctaacgta
     9961 gtggacatca aaacaaaaga agaatttgaa aaagatcaag aaacggtgca agacaaagta
    10021 actgacgcag cgaaaaaaac cggtgaattt gcttcgaatc aaacagataa agtaaaatca
    10081 gcagctggaa gcggcgcaca aaaagtaaaa gaaaatacag aaccacgggt tcagtaataa
    10141 ggaagtaaaa cattaggagg aacgaaacat gggatttatt tggtcactta tcgtcggagg
    10201 aattatcgga gcaattgcag gagcaatcac gaataaaggt tcttcgatgg ggattattgc
    10261 aaatgtcgta gcaggtttag ttggatcagc acttggtcaa gcattattag gttcttgggg
    10321 accaagtatt gcaggaatgg caatcgtgcc atcattgatt ggtgccatca ttttagtact
    10381 aatcgtatca ttctttgctc gaaaactgta aaaagacttt taaaaagagg tcgtgaaaaa
    10441 gctgttcttc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt
    10501 tggttgggca tattatcgaa gatgcaagct tttgaacact gtttatacgg aataagaggc
    10561 tgtgacaaga cttttgtcac agcctcttat ttatgcgggc aaattcccaa ttagattttt
    10621 aaaagttttt ccttaaatgt gtctttattg atacagagaa gcttctttaa taaaacaatg
    10681 cctccctaaa ttttcttgag ataggaaagg tgaacataca aaataatacg accactttga
    10741 aaacccagat gatccacaaa tggatcgtat gatactgatg aagtaaaaag caatggatca
    10801 gaaactcaaa gagattgaaa aagacaagga agaaataata tctctttttt actcttgtct
    10861 gttttagcca ataatagctc gtaaagtaat ctagacaaac aaatagaagt aaacaaagtt
    10921 tcgtaagagc agcaatcgta tcatgttgct ctgctagacg attgacgagc aggaaaaatg
    10981 ttggtaatgg aatatagatc acgagtaatg aaagaaaaaa gtaaacaata aatggcatac
    11041 tattcctcct aattgtgtga gttcgccctc attttaaaat aacgatattt gtttgtgaag
    11101 ctttttc
//
LOCUS       DACDEA010000082        11020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDEA010000082 DACDEA010000000
VERSION     DACDEA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.083"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(112..381)
                     /locus_tag="IV136_002293"
     CDS             complement(112..381)
                     /locus_tag="IV136_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8466260.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(371..637)
                     /locus_tag="IV136_002294"
     CDS             complement(371..637)
                     /locus_tag="IV136_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8466261.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            complement(706..933)
                     /locus_tag="IV136_002295"
     CDS             complement(706..933)
                     /locus_tag="IV136_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8466262.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            complement(1138..1770)
                     /locus_tag="IV136_002296"
     CDS             complement(1138..1770)
                     /locus_tag="IV136_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8466263.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            complement(1783..4506)
                     /locus_tag="IV136_002297"
     CDS             complement(1783..4506)
                     /locus_tag="IV136_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466264.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            complement(4643..5608)
                     /locus_tag="IV136_002298"
     CDS             complement(4643..5608)
                     /locus_tag="IV136_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8466265.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            complement(5662..6474)
                     /locus_tag="IV136_002299"
     CDS             complement(5662..6474)
                     /locus_tag="IV136_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8466266.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            complement(6488..7261)
                     /locus_tag="IV136_002300"
     CDS             complement(6488..7261)
                     /locus_tag="IV136_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466267.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            complement(7275..7745)
                     /locus_tag="IV136_002301"
     CDS             complement(7275..7745)
                     /locus_tag="IV136_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466268.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            complement(7742..8158)
                     /locus_tag="IV136_002302"
     CDS             complement(7742..8158)
                     /locus_tag="IV136_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8466269.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            8483..9325
                     /locus_tag="IV136_002303"
     CDS             8483..9325
                     /locus_tag="IV136_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8466270.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            9720..10703
                     /locus_tag="IV136_002304"
                     /pseudo
     CDS             9720..10703
                     /locus_tag="IV136_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(10784..>11020)
                     /locus_tag="IV136_002305"
     CDS             complement(10784..>11020)
                     /locus_tag="IV136_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8466271.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFFSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         3451 a         2150 c         1756 g         3663 t
ORIGIN      
        1 taaaaaagca gaaaccaacc gatagtatcg ggtagtttct acctgtggta ttatgcaaaa
       61 ttcaaaagat tatacattga ctttcgaaac tctacttcgt tccaaaaacg gttatctttt
      121 atatattgta cttctatgtc caattgagaa tatctggatt tcaatttcag aatcacaaat
      181 attagccaat attcgatagt ttccaattcg atagcgccat tccccactct tatttccagt
      241 caaacctttt ccgtgctgac gcgggtcagt cgtgttttct aaattctttc tgatccaggc
      301 gacaattatt ttagcttggt ttctgtccat tttttttaat tgcttctggg cttgcggcat
      361 gtagatgact ttataattca tttagcagtt tctccatttc ctcttgagaa ataccactct
      421 cttttttctc tttggaaact tcaattgcac tccgtaaagt ccgtagatcg atttcatctt
      481 cgattttatc cagtacagca tttcgataaa gttcggtcaa tgaagaattg tgtgcttttg
      541 catattctaa taccagttct ttttcatcgt cgggaatcct aaatgtaatg gttgattgag
      601 acatgattct acctccgttc atcatttgta ctaacattgt actaaataaa atcctttttg
      661 tcaatacaac gttagtacaa acaagatttg catcatatcc attattcaaa ttctttgaga
      721 tagcacggta tcgcaaaatc tctgactgat cgttttaaga agctggtcat tttgaagcga
      781 ttttctccaa ttttcaggga tcacttcacc cccgtaaaga atacccgcaa gagcaccagt
      841 tacggcagca gtagtatcag tatcccctcc taaattgacc gcagtcaata tggctgcttg
      901 ataactattc gtattttggt tctgttgcaa aatattctag caatccctag gatcattcac
      961 atgaccttag ggattgcgaa caaaataaaa aacttaaaaa gccaatccca ttatcactgc
     1021 cgaagcattt tcgtaaaagg tgttaccagc accttttact acacctgaat tatagcaagt
     1081 ttttttggtt agtcaaaata gctagcacag atatatagcc ttatttccat taattcacta
     1141 acctttttct aaaacaattt gttccagttc ttgtagactt tcaatttgta tgtctgcttc
     1201 actctgatcg atttgcattg gttgttttag tgccgccgta tagatatttg ctgcctttgc
     1261 tgatttaata ccaatcgttg aatcctctac tgcaataaag aagtcacagt ctaattgctg
     1321 tttcgctttt aaatatattt cgggatgtgg tttgctttgt tttaatgact caccacttat
     1381 aacaaaggaa aaatattcag taagttcaca ttgaatgagc atactctcta tctcgacagc
     1441 tggagaagaa gatgccagtg ccaatggtat atcccgcttc ttgaggaagt caagtaacgg
     1501 tttcacttct tttcgtaaag ctttagcgaa atcaattgga tgcgtttttc gataatctac
     1561 atatttcgga taaagttcct ctcttaatga tttgtcctca ggaactaatg tttcccatat
     1621 tgcagcttct gttttcccca cataatcatc aatctggttt gatcctggat ctatctccag
     1681 ttcttgaaag aagttcattc ttctctcaaa ataaaatgtc tcgctatcta tgagtacccc
     1741 atccatatca aaaattactc ccattgtcat tcgaattcac tcctatcctt gaacaatatc
     1801 gctcaataat cgcaactcta aatctggtat ttcaattgtg tattcccttt caatctcaaa
     1861 aaaagcgcta tgaattttat ccattatttc tccatttgct tgcatatatt cttcaatgtc
     1921 ctcttgctca tccacacttt ctttacgtac aattcgttca atcatacaac tacagtgaag
     1981 gtacaacaaa aacagctgat tattagaaaa atcaaaattt aataacttcg ataattcttt
     2041 aattgctttt tgtacaaaag atagcacctt atctggatcc aaaatggtta aattttcgac
     2101 aattttacta attgaaaaat tgaaaattaa ttctttttgt aaatcgttca cttcaatagt
     2161 cagctctttt tccaagtaat gaacaaattc gccaatgctt cccccattaa tcaggcgatc
     2221 taatccaata tatggaatct gattaatttt gggatcttct gtacctacaa tcatgatgac
     2281 atcgtataat tgaaaaggag tctcaaaatt cttgttttct aacagctttt tgtaatcata
     2341 cggaataatt actaattctc taactgcatc tttcaagcat ttcgttaaaa tctcctgaat
     2401 ctgggtcgca ctaccaatcc ctgtataaca acaagtaatg atggctttct ttttcttttt
     2461 cttgggaagt agtaactgct ttttgaaagt aagacttgtt tcaatttctg ctcctatttc
     2521 ggtgatactt ttattgttta atacttgttg gccgacttcc aataataacg gtgtattgat
     2581 atgatcaatg attaacagtg gtccatttag atgattcact aataaaccgc ctagttggtt
     2641 tagtgaaccc atatctacca ataaaatcaa gcctgaatct gttcgataat ctccaaggaa
     2701 ttgaatcaat tttgcttcaa ttttctctaa tgtaatatct aaaggcatat cataagcttg
     2761 aaaaacgttc ttattcagca tacggttggc aacattcgcc atactactag ctgtagaata
     2821 accatgagct agcacaatac tcttaatacc tgttttaaca ttcgtgacat tcaaactata
     2881 aaaatatagc gtaataaaga aaatgtcctc ttcatacaca ggttgatcta aacactcaga
     2941 taaaaggctt aagatttttt ttgcatacca ataaggggtt tcaagaacat tagcaaaaaa
     3001 ttcgttaagc tgatctttgg ttttcttttc ttgaagacta ttttcaaaaa tcacttgatt
     3061 atccttgaca tatagatagt tggccaaagc gactacccgg ttaccatctt gttcaaaccc
     3121 gtagctttct cgcataaaat caatactttg acgaatatga tagtttagaa tggcgtataa
     3181 agactgctct ttttcgtaat cctcttgaaa aataagcgta tccattactt gtgttacctt
     3241 ggtaaccatt tcttctaaaa atagtttaat atccttgtta ttttcaatca cttcagagaa
     3301 tccagtaagt aattggtcaa aaaattgaac aaaaagctga ttgtttcttt ctagttgcac
     3361 ttgtcgtgtt tcttctggat agtagattcg atctgctctt ttctttctgg gtttatttag
     3421 ttgctccttg aatttataac tgtactctaa aggcatatcc aataatttaa cttgaattgt
     3481 ttgctgactc ttctggtgga tatatgcgtt tccgcaagca tacttgataa tatttttaat
     3541 attccccacg ttaccttcaa aatcactatt cattaagaaa tgaatcaatg catcagaaac
     3601 agatagttcc ttagctaaaa tttggctctc agaaacaaaa aattcatcaa tcaatgacag
     3661 acgttcaaac gacggacgtt gttgaaatct aggtagatta acaataactg gcaatctcct
     3721 aagaaaagtt ggtaagaaag tcgtatatat attttctgtt gttgcaaaga ctaatcgaac
     3781 atcggctttt ttccgaatac tattatcccc cataggagag aattcacccg tatccataaa
     3841 tgtaaataat ttctcttgcc cttcttcaga taagcgatgc acttcatcta aaaacaatac
     3901 tcctccatcg gctttttcta gcaaaccagt atgctcctgg cttgctccgg taaatgctcc
     3961 tttcgtgtag ccaaataaag cagaggatag taactctgga ttgctcacat attgtgcaca
     4021 attataatct atgaaaggtg ccgatgtgtg aatgaccttt tcttctactg catattcata
     4081 tattttattc gctagaaagg ttttccctga tccactagcg ccgatcaata ataaaggcaa
     4141 cccttttcca ggatataaga ctgccgtttt aatttgatca attgcttctt tcaaacttcc
     4201 tttagagcca attacttgat ctaatgcggg tttttgagaa attgttaaca agctttcaat
     4261 tgattctatt tcggagttat tcagtgtgat atgtagttct tcaagagctg ttctatgtaa
     4321 aaataaaacc gggtaacttt ttacttttat gaaaagccct tcacgaacac cttcatttaa
     4381 gtagctactg attgtacttc tattcaactg tagagcttgc gaaatactgc gagctgaata
     4441 attatctgaa ggcgtagatt tcaaaagcaa tgttagttga taaagatagt catttaatcg
     4501 ctgcatcttg ccacctgctt tcacaatatg tactagttat tttaccatat gtactagtta
     4561 ttttaccata tgtataaaat cacattgaag aaaacaaggt ggcttaattt ctcaagccac
     4621 cttatttagt ttgatctatt ttttaataag ttagtttcca catataacgt ctcttagtta
     4681 atggatgttc tcttgcttct gacaattctt ctgcataaac tctaaatact gcagtaatca
     4741 cgaatgggtt aaagtagtct aagacttctt tagcaatatg tgaagacaag ccataatcca
     4801 aggcatctac aacttctgtt ttcgtatcaa agcgttcaag gaaagttaat gctcgagcat
     4861 ctatatttct agtttttcca tcattcatta ataatataaa cggtacgtct ttatccacaa
     4921 tttcgaatgg tccgtggaaa aattctcctg aatggaaggt accagaatta atccattgca
     4981 tttccatcat caagcaaatt gatgttgagt atgctacttc atgtgtagca ccactactca
     5041 ttacataaat aactttttca tctttaaagc gttcagcaaa tttcttcgca tctttacgag
     5101 ctgattctgc acttttttgg gcaagatcat agatagtagc aaatccttct tgcattgctt
     5161 cataatggtc ataaccttct acttgatgta aaagttctac tgccaattct aaacagtagc
     5221 ctgttttttc taatttagca gcatagttct ctgcaaatcg atgataaatt acataatctg
     5281 catcttttgt taacggtgag tcaatgcttc gtgttagact aataactgta gcaccttttt
     5341 ctttagctac tgcgttagct tcaactgttt ctggagtcgt acctcctaaa gaagctgaaa
     5401 taacgacagt tgactcatct acagatgctg gtgttgcata gttaaattcg ctggctgtgt
     5461 aatgactaat ccgtaactgc ttcgcatttt ggtctagaaa atactttgca ggataaaggt
     5521 ccgcttttga agcgccacag ccaacaaata cgattgattt tacctctttt ttcgctacga
     5581 tttctttaac aatttctttt gaattcatca attgaattcc tccttattta tattattcaa
     5641 ccaaatttat tggttattcc cttattgtaa gatacctaac aaactaaaaa taattcctcc
     5701 gactaaacag attgccaaca tccatcctgt tttgaccttt ttctgcaata accagtacat
     5761 gaagaaggtt aatcctaatg gtaaaaattg tggcataata ctatctaata catcttgtaa
     5821 gacaaaagct ttacccattt gaattggtgt tgtaatcccg atcatgctgg caatcatcga
     5881 accaatgacc atcaacccca caatggagca aacatacatt actttatcca tcaaaccttc
     5941 ttgatacagt ttagacaagt atttattccc catgttatag ccaattttcc caccatagta
     6001 agtaactaaa aaggaaggta caaaggaaat taacattgct agaattggac ctaaaatact
     6061 accttgttgg gctaaattaa ttcctagtcc aaaggctaat acccgaatcg ttccttgaaa
     6121 gaaagaatca ccaacaccag atagcggtcc catcagagaa gtcttcactg cactgattga
     6181 ttccggatta aagttttctt ggtcttcgta gtattgctcc tccatagaag cagttaaccc
     6241 taacacaaat gcacttgttt gaggggtaca gttgtagtac atcatatgcc gctcgtaagc
     6301 ttctttcttc attgcttcat cttctggatt cttgtaaatg ttatctagaa ttggtgcaat
     6361 tccgtacata aaacccatgt tcatttgtcg ttcatagttc catgaggctt gaattgccca
     6421 tgaacgccat aagaactgcc gatatttcca tgcattttga tctttctctt tcacttaatc
     6481 tacctcctta taagtcatct aatggatctg gctcattttg gttaccattt gagtttttac
     6541 cttgaacttg aaccatgaca aacgcaacaa tacatgcgaa ggctgcaata ccaatgattc
     6601 ccaatccaga ataagcagta attaaaaatc caagtaataa aaatggcacc atttttttcg
     6661 ttaacatcgt ttgtaataac aacgcaaacc catatgctgg taaaatttta cttgcaaccg
     6721 ttaagccatt tgttagccac tcaggaatcg catcaacaat ctgagaaaca agattcgttc
     6781 caaaataaat tgctagaaaa ataggaataa aatacatgag agcatataat cccgagccat
     6841 aaagtatatg aattctcctt gcttggctaa attttccttg ttcgataaag cgatccgcaa
     6901 cgtgagtcaa atttgacaaa atcgtccgca ttaagatacc tagtatttga cctaacactg
     6961 aaattggaat ggcaatcgtt aatgctgctt ctgcattcgc atttgataaa atggcaaagg
     7021 ctgtagcaat aattgatgcc atattcatat caggtggtga agcagctccg atatttacta
     7081 atcctaagga catcaattcc aaagaggctc caacaattaa tcctttttcc atgtctccaa
     7141 gtaccaaacc aacaagcgta ctgacaatca acggtcgttc aaaattcatt cttcctaaaa
     7201 gccttgagtc taaaatacaa aaaacaccga ttaaccctaa accaattgac tgaacgagca
     7261 ttttcattcc tcctttataa cattgtattc aaatcttctt ttttagaagt tggtacctgt
     7321 tgaataaatt gtttaatgcc aagagctttt aattttttgg catcttcttg ctcagattca
     7381 ttcaagaaaa tggcattact aaattgcttg gaaccttctt ttttgattat ccctccatag
     7441 ttgatttctt gtactttagg ataggcttca caaaattgac gaatttcttt tgtattacca
     7501 aaaataagca tgatattttc gttaagtgtt tcgattttag gcattttttc tagtaactgt
     7561 tcaactgaaa aaattgttaa tttaatgcca gtcggtttag ataaattcaa agacattttt
     7621 ttgaattcat cattcgctgc ctctttattg gcaataataa tacgattgat atttaatgat
     7681 ttactccaag aaaatactac ctgtccgtgc aacaaacgat ggtcaatacg tactaactta
     7741 atcataggtc atctcctcca gtaagttgtt ctactaataa ttttctacaa tcaatcactg
     7801 attgttgact ctctttaata agcttttcta gctcactttc cccaattttt ccattatagg
     7861 ttgcaataga aataactaaa ggtaagttca ttccagtaat taattgcaat ccctcatact
     7921 ctttaataat agtgagcata tcattattca cactaccacc tagaatatca gttaaaatgt
     7981 aggtttcttc tatatctatt gtggctaaaa gtttttttat ttgtaccaaa atagattcat
     8041 cttcatctct aaatgctgac aaggcatgaa ttggtacact atctccaata atcattgtcg
     8101 ctgtttgctt taatccttga gcaaattccc catggctggc aagaattata gttgccattt
     8161 tcattcctcc tatccttacg cttttctata agcaagatgc gtgccaattt taggaaacgc
     8221 tgttaattaa aggtttttct atatcatgcc aacacttttc tttccctaaa ttatagtgtt
     8281 gatgaaagtg tgggtattta aaggtgtcta attaaatgat cactgtaatt ggaatttagc
     8341 tttgccccaa tcccccgcca catcctaaga aattttcttc cacatacgaa ataaagcgta
     8401 aaaagcgtcc attaatattt ttttcggtaa ttacaacaaa aaatagaact taatgtgcta
     8461 caatatatga atacacgttc atgtgaaagg agatgttata gtggaaatca aggttttatt
     8521 tgaaaactca agaataaatt tgaactttaa aagtggacat gggttatctc tcttaattaa
     8581 ctactcaggg aagaaatatc tcttcgatgc tggcgctaat aaaaaattag tctataatgc
     8641 gactaaaatg ggggaaactt tgaatgatct ggaggcagtg attatttcac acggtcattc
     8701 tgaccacacc ggcgggttaa gatatttaaa atcaggaaac gcccctattt atacagctga
     8761 aggtgtaact ttaccccatt atttaaagat tttaaccacg tttctgtatg ttggtattcc
     8821 taaaaatgaa atccttaaaa tgcatgagaa tttacataca ataaaaacga aaacagaact
     8881 tgctccgagc ttccatatca tccccctagg caaagcaaac tcgaccacca agaatttata
     8941 caaaaagatg gatgataaat ttgtttatga tgatttttca gatgaattat tggtcgtaat
     9001 tgagcaagaa gacggtctga ttgtctttac tggctgtagt catcatggca ttatcgatat
     9061 gatggaaatt gctcttgaaa catttccgac taaacccata aaatacctaa ttggtggctt
     9121 ccatatgatt ggcatacctt atataaataa tttaggtcta cctgagcaag aggtaatgaa
     9181 aattggaaat aaattgaatg actcacgagt agaaaaaatc cttacttgcc actgcacagg
     9241 ttcaaaagcc ttcaacatat taaaaacaca gttaggaaac aagctagaag ctattaaaac
     9301 tggacagcac ttagagataa gttaaaagag aaactgatag gactgtcgag aggacaaaag
     9361 ctatatgaaa taccccctct aatgtttgct ggcgataaca caaaaggcag accttctgtg
     9421 aatagggcta cctttttgtg ttttttcact aactttcaat atcttggtca ttcaaggaaa
     9481 atcacctata tttctgtgtg ggtttacttc cttattaaat cgagtcatct atttcgttta
     9541 ttcacttttt caagtggaca ttcattaagc tgtttatggc agaataaaag tacatacaag
     9601 tgcaacgaaa aactacacaa ctccgcaaat cgattaagtt aagataaaaa gccattgcgg
     9661 gcaacctgaa actcctctaa agttatagtc tcgacactgt aactggaggt aagaaaggta
     9721 tgctcacaat gaccgatatt aatactatca gaaatttacg aaataatcat gataaatcca
     9781 ttaatcatat tgcaaaggag ctagatgtaa attggcgcac ggctaaaaaa tatgcagatg
     9841 atgatttgct gccgcaacct aaagcacgca aaaagactgg tatgatgtat acagaaaagt
     9901 ggggaggcat cgtttcccta tggctttcag aagatgctaa gctgccaaat aaaaagcgta
     9961 ggactgctga agcaatgact actgctttaa aagccgatgg ctttccagga tcctaccgaa
    10021 cagtctgtgt attcattcaa gattggaaag cgacacacta cttggaaaca ctggaagacc
    10081 aaggctttga gcgtcttgaa cacccgccag cagaagcgca acttgatttt ggtacgatgg
    10141 aagtttgtca cgatggagcg tttaaagatg tgaaggtatt agtgatgact ttccctttta
    10201 gtaatgcagg attcgccgtt gtactccccg ccgagaatca agaatgtttg ttggaaggca
    10261 tgaaagaact cttcagccaa tcaggtggcg ttcctcggaa aatacggatt gataatatgt
    10321 caacggccgt tacttagaca aaaacaaaga ctgaacaagc ggtgctgact gacggtttcc
    10381 tccgattttc tatgcactat ggttttgaga cacaggtgtg caatccacgt agcggaaacg
    10441 agaaaggtag tatggaaaac aaagtcggct atgtacggta caacttcttt tcatctacac
    10501 cgattatgaa ggatttcgcc agtttcaacg aggcattagc ggtggagctg tcacacgatc
    10561 gagagcgaat ccattacgaa aaacatgtca ttatcaatga tctatggaac acagaaaaaa
    10621 cagatttgct agctttgccg gacaccgatt acccagtatt taaagaaatc gaagtcaaag
    10681 ccaataagta caacgaaatt aaacaaaaca aaataacaaa tttatcgtag ttcaatatac
    10741 ttgatagaaa aactatttat tttacccgtg gtgctagtta attttaatat cctaaagaat
    10801 atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta
    10861 atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac
    10921 ctaatttttc taaggaaaaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc
    10981 tgtggtctag tttttctgat ttatccatgt ttttacgtgg
//
LOCUS       DACDEA010000083        10637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDEA010000083 DACDEA010000000
VERSION     DACDEA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.084"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(180..1028)
                     /locus_tag="IV136_002306"
     CDS             complement(180..1028)
                     /locus_tag="IV136_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8466272.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1192..2094
                     /gene="gnd"
                     /locus_tag="IV136_002307"
     CDS             1192..2094
                     /gene="gnd"
                     /locus_tag="IV136_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8466273.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2096..3097
                     /locus_tag="IV136_002308"
     CDS             2096..3097
                     /locus_tag="IV136_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8466274.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3094..3378
                     /locus_tag="IV136_002309"
     CDS             3094..3378
                     /locus_tag="IV136_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8466275.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3411..4208
                     /locus_tag="IV136_002310"
     CDS             3411..4208
                     /locus_tag="IV136_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8466276.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4222..5055
                     /locus_tag="IV136_002311"
     CDS             4222..5055
                     /locus_tag="IV136_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466277.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5073..5930
                     /locus_tag="IV136_002312"
     CDS             5073..5930
                     /locus_tag="IV136_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8466278.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5952..6461
                     /locus_tag="IV136_002313"
     CDS             5952..6461
                     /locus_tag="IV136_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466279.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6498..6899
                     /locus_tag="IV136_002314"
     CDS             6498..6899
                     /locus_tag="IV136_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466280.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7011..7364
                     /locus_tag="IV136_002315"
     CDS             7011..7364
                     /locus_tag="IV136_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466281.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7398..7841
                     /locus_tag="IV136_002316"
     CDS             7398..7841
                     /locus_tag="IV136_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8466282.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7858..8943
                     /gene="uxuA"
                     /locus_tag="IV136_002317"
     CDS             7858..8943
                     /gene="uxuA"
                     /locus_tag="IV136_002317"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8466283.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8936..9649
                     /locus_tag="IV136_002318"
     CDS             8936..9649
                     /locus_tag="IV136_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8466284.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10401..10601
                     /locus_tag="IV136_002319"
     CDS             10401..10601
                     /locus_tag="IV136_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8466285.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3551 a         1685 c         2309 g         3092 t
ORIGIN      
        1 atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg atgacatacg
       61 ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa aagccactac
      121 caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct gttatggtgc
      181 tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg tgattctttt
      241 tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg agaaatacgg
      301 gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa aacgacatcg
      361 ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt tgctccagtt
      421 tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg agaaatcaaa
      481 acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg gtaatccgtg
      541 ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc
      601 tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat agtagccgct
      661 ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga aatactagac
      721 tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt ttcgtggata
      781 gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc tttaaatcca
      841 ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc cagttcactt
      901 gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat ataatcggcg
      961 atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag tcttatggag
     1021 acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt tctcattaat
     1081 gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt aaaatctatt
     1141 tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat catggaaatt
     1201 ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa agagcaccat
     1261 cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag agaaaatgac
     1321 attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa aaaaatcatc
     1381 tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact ttttccaaaa
     1441 ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga ttcaatccgt
     1501 cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac ttctggagga
     1561 atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga aacttttcta
     1621 tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta tgctggtgca
     1681 tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa
     1741 gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga cttggaacaa
     1801 gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga aatcatggaa
     1861 gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc tgcatcaggt
     1921 gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc agtaattgca
     1981 actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc aaaagtagtt
     2041 tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga gtaaaatgcc
     2101 agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc cacaccactt
     2161 ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag agattccagg
     2221 aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg ttacgccatt
     2281 ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc gacatggtat
     2341 tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg tatctatcat
     2401 acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt tgttgtccat
     2461 gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg aagatcgggc
     2521 ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg caggaaatac
     2581 cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga
     2641 tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag aacttcaaga
     2701 attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag agaactatca
     2761 catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat ctaatagtgc
     2821 aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc
     2881 aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc cgtatttagc
     2941 gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat gtttagaagc
     3001 aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc ttcctgaacg
     3061 ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt gaacggttta
     3121 aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc aaaagtttct
     3181 gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac ggtatcatgg
     3241 gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt tttttatcat
     3301 cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat catcaaaaaa
     3361 atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta gtgggttata
     3421 gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt aattgtgctc
     3481 catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg aaaacagaac
     3541 acttttacag acaagctgct ttttttataa cagatcccca attagtgatt gattcccctg
     3601 ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa tctgagctca
     3661 tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa gatactgcga
     3721 agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat atggcacaaa
     3781 gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt gctggtttag
     3841 ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg catgacgcta
     3901 tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta gcgtatggga
     3961 ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta tatcctttgt
     4021 atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg aaagataaac
     4081 agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat ttgattccta
     4141 tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt tatatcagaa
     4201 agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt gttgattgct
     4261 ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt gaccttacgt
     4321 ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt tgcacaagcg
     4381 atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc aggaggatca
     4441 atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt attaggggat
     4501 ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg aagttacttg
     4561 tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa agctgtagaa
     4621 gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt tgcctcattt
     4681 ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt gatcgctgat
     4741 gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt aggtggaggg
     4801 ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga ttatctggtc
     4861 tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat tacgatggtt
     4921 acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca aaaagggaaa
     4981 caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga agatgactat
     5041 gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat aatcaaacag
     5101 gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga tggcattttg
     5161 caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta tacggaatga
     5221 tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca tataaacggc
     5281 agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact ggcctaatgt
     5341 cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc ttgatgcagg
     5401 atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga atcggagatg
     5461 caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct tgggcacaac
     5521 agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat caggttttac
     5581 taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca ggcctaaagc
     5641 aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg tttatgatgg
     5701 gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg atatccggac
     5761 gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta cctttagctg
     5821 tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca caagcattat
     5881 tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa aaagaaggga
     5941 acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc
     6001 aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt gtagacgaag
     6061 ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc cgtacaggac
     6121 aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa tatcaattta
     6181 aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac
     6241 acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat cctgatacag
     6301 atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa
     6361 tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct gcacctaaaa
     6421 caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct tagaaaaaag
     6481 ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa tttcgttatc
     6541 gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt tcagcattag
     6601 tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat acgctagcct
     6661 accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt atctgtactt
     6721 attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg aaagaccaaa
     6781 ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt ttaggttggt
     6841 ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta tccgtataaa
     6901 caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa gcagctcgcg
     6961 gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg atgctagaaa
     7021 tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg
     7081 gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt cttttttatc
     7141 tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt tcaaataaag
     7201 aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat atttttagtg
     7261 caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga gggaaaacat
     7321 ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa agtgcaaaac
     7381 aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag ttgagcgaag
     7441 gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa acaatgtgct
     7501 tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa ttaactatga
     7561 tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc
     7621 aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact tatgtcatag
     7681 ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt cacacaccga
     7741 tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact gtttggcatg
     7801 cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc tgaaaagatg
     7861 aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa atatgttcgc
     7921 caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc tggagatgtt
     7981 tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg actggaactg
     8041 cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga tgaacgagat
     8101 tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg
     8161 atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac
     8221 aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat ggagccagga
     8281 gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg ttgggaagaa
     8341 gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac cgaagaagaa
     8401 ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga aaaagcagat
     8461 gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt tcctagagtg
     8521 acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc atataatgga
     8581 ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt tgaaatcatc
     8641 catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt tttaggagaa
     8701 agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat gtatcatatt
     8761 atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca tggtagaacc
     8821 gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat gggactcact
     8881 tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga gcattatgaa
     8941 taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg gacgtttagc
     9001 ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta aggaagtcta
     9061 ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca ttttgtacgg
     9121 agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag aaaaaatcgt
     9181 gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat cttttgcaaa
     9241 agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag tagaaatgta
     9301 tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg atgatgtaaa
     9361 aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga atattcctcc
     9421 aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa ggcgagcggg
     9481 cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata atgggaagca
     9541 taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg aattttatga
     9601 cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat aaagaggact
     9661 attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg aaaaagaagt
     9721 aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac aaacacaaaa
     9781 cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa tcgtccatat
     9841 gagggtttcc actccttatg cagatttttt taattcttgc actgcttgtt cgatcgtaac
     9901 acctgtcgct acatgattgg tattttcttt gtcaaaaacg acaaatagat tcaaatctgt
     9961 atccaaggtt actcgatatt gtatatcttt gttatagaat gtcatagcca ttctcctttc
    10021 tttaaatact tgctgaaagg caacgaacgc gttgataacg aaattccatt tataagtata
    10081 ccaccaattg aaaggttttc atagttaaga aattttaaag tcaaaagttg cagataaaag
    10141 caaataaaca agaaaacaaa aagtgaatca atcgcggtta ttcaactttt tcttcgattc
    10201 gaagacaata gaagacaatg aattttatat ttcattgcat tatccttgtg ttattagaaa
    10261 aagcatgata tattaaatga gtaatctatt ttgaaacgta atttgagcga tatatctaca
    10321 tagataaaac tccttttgcc tatcaaatat ttattgcaac gaaaaaacgt acattgatac
    10381 gtatcaggag gaatttattt atgaataacg gtacagtaaa atggttcaac gcagacaaag
    10441 gttttggctt tatcactggt gaagatggaa atgatgtatt tgcacatttt tcagctatcc
    10501 aaggtgacgg cttcaaaact ttagaagaag gccaagcagt gtcttacgat gttgaagatg
    10561 gtcaacgtgg ccctcaagca acaaacattg ttaaagctta ataaaaattg acttttaacc
    10621 gtcttttcga aagaaaa
//
LOCUS       DACDEA010000084        10604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDEA010000084 DACDEA010000000
VERSION     DACDEA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.085"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            276..2222
                     /locus_tag="IV136_002320"
     CDS             276..2222
                     /locus_tag="IV136_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8466286.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2225..2680
                     /locus_tag="IV136_002321"
     CDS             2225..2680
                     /locus_tag="IV136_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8466287.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2694..4022
                     /locus_tag="IV136_002322"
     CDS             2694..4022
                     /locus_tag="IV136_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8466288.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4056..4340
                     /locus_tag="IV136_002323"
     CDS             4056..4340
                     /locus_tag="IV136_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466289.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4342..4857
                     /locus_tag="IV136_002324"
     CDS             4342..4857
                     /locus_tag="IV136_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8466290.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4873..5535
                     /gene="rpe"
                     /locus_tag="IV136_002325"
     CDS             4873..5535
                     /gene="rpe"
                     /locus_tag="IV136_002325"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8466291.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5542..6114
                     /locus_tag="IV136_002326"
     CDS             5542..6114
                     /locus_tag="IV136_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8466292.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6301..7821)
                     /locus_tag="IV136_002327"
     CDS             complement(6301..7821)
                     /locus_tag="IV136_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8466293.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8064..8249)
                     /locus_tag="IV136_002328"
     CDS             complement(8064..8249)
                     /locus_tag="IV136_002328"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8466294.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8233..8415)
                     /locus_tag="IV136_002329"
     CDS             complement(8233..8415)
                     /locus_tag="IV136_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466295.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8656..8874
                     /locus_tag="IV136_002330"
                     /pseudo
     CDS             8656..8874
                     /locus_tag="IV136_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9369..9968
                     /locus_tag="IV136_002331"
     CDS             9369..9968
                     /locus_tag="IV136_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8466296.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10032..>10604
                     /locus_tag="IV136_002332"
     CDS             10032..>10604
                     /locus_tag="IV136_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466297.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3501 a         1670 c         1982 g         3451 t
ORIGIN      
        1 atacgaattt tagtgacgcc attatattgt ttttattttt caattgtaat atttcatatt
       61 acttccataa taaggagtat tttctattgg aatatcttag ctcttttaag gatttctcct
      121 tttttgtttc tcatttttta agattatttg gatttccttt acaacaaact tccacaagta
      181 ttattgacat agatgtaatt gagggaaggg ggttccagtg ctagaataaa taatgaaaag
      241 aggtaacaaa tgctagcgtt aagaggtgaa caagtttgat tgaaagacat gtaaatttat
      301 taaaattatt aagtcgacaa gaagacttta aacccgcttc tttttttagt gataaattgg
      361 cagtgtcgac caagaccatt tatcaagata ttgaattgtt ggaagattat gtagttaatt
      421 accaagtaac aattgagcga ttaccttctt tgggtatcaa actcataggt gataggaaaa
      481 acgttgaaca tttgatttca tcggtagaaa attatcttga aaaagatatt tattctccag
      541 aaaaaagacg tgtcgagatc attaaagaag ttttgtttgg tgataagcct gtttctttaa
      601 ctcatttgtc tcaaagtttt atggttagta aaacatcgtt atacaatgac ctgagaatta
      661 tcaataaaat tattggcagt ggagaagtta agttgcagtc atctgcagaa ggaattagtt
      721 ttactggtaa tgaacaagca attcaacgag caatcaagca acttgtattc tattatgcga
      781 atgacatgga atcaaaaaca ttttttcaag ttttaaatat gttgtttgat tcaaaagtta
      841 ttagtgtcat tcatgatttg ctattcaatg attacagtga actgactgaa aaggtttctg
      901 attactatgt tcggtcattg atttctactt tagttatcca atgtaaccgt ttactgaaag
      961 gtcatcattt aactgttgaa gaagaattcc tcttcaatag cattcgctat atggaaacgt
     1021 acattgtggc aaatggttta attgaagaat tgaaggcaaa attgaatctt ccattttatg
     1081 acgcagataa agaatatcta tgtcgtcaac tatttgcaca tcgtgtgaca aataatttaa
     1141 aaaccagcaa tgaagagtac gctatgagcg ttcataaatt gattgaaaga atgagtgaga
     1201 ttgaaaaaat tgatttgact caaaatgatc gattatatca atccttattg tatcatgtgc
     1261 cagcaatggt ccttcgttta aaaaaaggta ttaaaattca aaatccgtta ttagaaaata
     1321 tcaaatctca atattcagaa ctctttagca tagtatggta cgccatgtcc atccttgaag
     1381 aagaatttga tgtgacttta acggacgatg aagtttcctt aattttgatt cattttcaga
     1441 ttgctattga agatcagtcc aaagcaaata atatcatcat tatttgccaa tacggtattt
     1501 cttctgcgca attaatttac aataaagttc gacggttttt accagcaaaa gataatgtag
     1561 aaatatcaac tttagataag tttcatttag ctgaaattgg ggaagttgat ttgattattt
     1621 cctctctttc ctttgatgca ggtggtatac ccttagtaag ggtatctcca ttggtaagta
     1681 aaacagatta tatgaatatt atggaagctt atactaaata tttaatagaa gaaccttcgc
     1741 gatttattca aaatgaactg attcctaaag cggttaaagt tcccaccata tctaaatttg
     1801 ttcaatcatc tttagtaaaa acaggagtac aggtggaaag caaagaagaa tgtttggatt
     1861 tgatgatttc cgaactagaa gccaattcat tagttactga agagtttcgt gcttctatct
     1921 tctctagaga gaaaatgggg aatactaatt tagaaagtgg tgttgcttta ccgcatgcga
     1981 atccatcaat ggtcattgaa tccaacattt ttattgtagc attaaaaaat gcgattaatt
     2041 ggggcgatcg aaaagtcaaa ttagtcatta tggttagttt agctgaagaa gatgtatctg
     2101 agattagagg agtatatgaa gaactttacc aatttattgg gcaaaaagaa atggtcgatc
     2161 gcttgttaca agttcatgat agcgaaaaat tactaagaat ttttcaacaa aaaggagaat
     2221 aaaaatgtat ctagataaag aattatgttt atttcaatta gaagtgaaag atcaaaaaga
     2281 attgtttcaa gtaatgtctg aacaattaaa aaacgctggt tgcgtgaaag atagtttctt
     2341 agaaggtatc accaaccgtg aacaagaatt tccaactgga ttagaagtaa atcaaattgg
     2401 gtttgctatc ccacatacag attccgcaca tgtaaatagc tctcaaatct gttttgcctc
     2461 attaaaggaa ccactggtat tttcagatat gacagataaa tctcatgaaa tacctgtacg
     2521 tttagtcttt atgttggcaa tgtcacaacc tcatgaacaa attgatacac tacaaaactt
     2581 agttagccta tttcaaaatg aagaaaaagt aaatgaatta cttgcatgta ccacgaaaga
     2641 agcatttatt gagttagtac attctgcagg agtttattag gaggaataga gagatggata
     2701 ttgtattaaa tgcctttgaa tggtttattg gattagggtc aaacgtcttt ttaccactga
     2761 ttatttttgt aatcgggata ttatttggat taaaacctgg taaagcattt gtttccggcg
     2821 ttactgttgg tatcggttct attggtttag gattagtttt aagtttatta tcagatggtt
     2881 taggaacagc aattcaacaa atgggggaac aatacggtgg tggcctgaat atcctagata
     2941 ttggcgtagg tgttggtgga cctttggctt actctacatc attgggaatt ttaatgattc
     3001 caatttcatt aattcttaac ttcatatttg tggttttggg ttggacaaaa acattgaatg
     3061 ttgatatttg gaacttttgg ttcgcaattt tcctaggtat gattactcaa tctgttacgg
     3121 gaaacttttg gtttggctta attggcgcga ttgtcgcagt aattttacaa tggttcttag
     3181 cagatcgtac acaaaaaact gttagcgaat ttttcggcta tccaggaatc tctatcacac
     3241 atatgatggg attatcagga gtactatttg ccaaaccagt aaattgggtt tttgatcgta
     3301 ttcctggatt taataaaata gatgcagatg cagaaacatt aacaaaacgt ttcggtgttt
     3361 taggtgacac agttgttatt ggcctaatta tcggtattgt agtgggtatt atggcaggct
     3421 atgacgtagc aggaattgga aacttaggaa ttatgactgc ggcagtgatg aagattatgc
     3481 ctaaaatggt agcaatgttt atggaaggtt taatgccaat tgcagaagcg gcgaaagaat
     3541 ttacgaataa aaagttaaat ggtcgccaag taaacattgg aatggatgca gcgttaactg
     3601 tgggtcatcc aactattatg tcaacttctc tattgcttgt accaatctca ttattgctag
     3661 cactaattct tccaggaaat aaagtattac cttttggaga tttagcattc tttgctttcg
     3721 ccatctgttt aatgattcct tattttaaag gaaatattgt gagatcatta gtaggatgtt
     3781 cgttgtatgt tgttacaaca ttatatatgg caacatggtt ggcaccaatt atcactgatg
     3841 tgtttacatt agcaagtttc gatgtgggga cagcaggatt agtaactatg ttgttatgtg
     3901 gattatggcc agtagggttg tttgttttat tagcatctta ctttggtgta attggaatag
     3961 gtggattagg agttgtttta gtaggtgctt ctatctacat gaacaaagtg aagaaagctt
     4021 aatcataaaa ttaaaataag aaagaagaga tcaatatgaa aaaattatta gttatttgtg
     4081 gagcaggtca tgctacttct acaattgcag tagcaaaatt atcaaaatgg ttggaagaag
     4141 aaggattaac agatcaagtg aaaatttatc aatctaaaat tgctgatgag ctaaataaaa
     4201 ttgatgaata tgatgctgta gtaagtacaa caattgtccc tgatgtaatt aaagctaaag
     4261 taacgaatgg cttgggctta ttaacaggta ttggtacaga tgccatttat gcagatgtaa
     4321 aagaaagatt aggtgtttaa tatgcgtttt catcatgaca ctttgactat tgaaacagtt
     4381 tcagggcgtc cttcgtatca tgatattcga aaagaacttt tagatacagt tgatacagca
     4441 ggtattgaga atggaatgtt agtaatttcg agcccgcata caacttgctc actctttttt
     4501 gaagaaacga tgcatgacac aaactttttt ggtgatgatt atttacatgt agacatcaac
     4561 aatgtaatgg aaacgattat tccaaaaatg acgagtgaaa atcaatacaa cagtccgggg
     4621 ccaaaacaca ttgattttgg aacaagatta tctgatccca attaccctgc agaaaaatgg
     4681 gtcatgctaa ataccgatgc acatttacgt tcatctattt atggaaccaa ctcaatgact
     4741 ttaattgttc aagaaggtaa acttttatta ggagaaatgg gccgtgtcta ttttgtcgat
     4801 tgggatcaac taagagagcg aactagaaca atcaatctca tgattatggg agaataaacg
     4861 ggggaataga gaatgacaat tgctccatca ttgttaaatg cacctatcgc agaagttaaa
     4921 gagttatttg aaatcttaaa agaaaagaat gttgattatt tgcatattga tgtaatggat
     4981 ggctgttttg taccagatat ggcttttgga ccaagtgtta ttaaagatat tcgtccacta
     5041 acagacgttg tgtttgatgt tcatttgatg attgatacac cagaaaaaat tgtcacaaga
     5101 tttgtagaag caggagcaga tattgttacc ttccacattg aagctaccaa acacgcaatg
     5161 aaggtcattc agttagtcaa ggaagcagga gcaaaagtag ggattacttt taatcctgca
     5221 acaccagtca gttttattga accagtatta tctttagtcg atcaagtatt agtaatgaca
     5281 atcaatccag gaacagagaa taagcatttt attcaagaga cagtagttaa aatcgaacag
     5341 ttagatgtaa tacgcaaaca aaatgattat acgtatgata ttgaagtaga tggcaagatt
     5401 gataatcaaa cgattaaagt ttgttctaaa gcaggggcgg atatttttgt atcaggcggt
     5461 ttcatcttca atgggaaaaa taccccgcaa gaacaaattg ataaactaaa tcaatcatta
     5521 gaggaagtga agtaaaagat catgaccaat aaagaccaaa ataaaaacta tttagaagcg
     5581 attatcttag aattacaaaa taactcaaaa cgtattaatt ctgtagagtt agaacaattt
     5641 agtgagaaaa ttgttcaagc taaacacatc tttctagctg gtgcaggacg ctctggaatt
     5701 gccatgcaag catttacgaa tcgcttaatg catttaggtt tttcagtcag ccttgtcggt
     5761 gaaattagtt caccacattc gcaacctgga gatttattaa ttatctgttc tggctcaggt
     5821 gaaacaggta gtttaaaaaa tttagcggcc aaagcaaatc aaagtggtgt aagcattgct
     5881 ttagttacca tgaaaaaaga ctcgtcgatt ggtcaattag cagattctgt tcttgtatta
     5941 ccaggtatta ccaaagatga aaatcaacga gaagaagata ctttttcaca acctatgggt
     6001 tcaagttttg aacaattagc atttctcacc tttgatagtt tagtgttatc tttaatggat
     6061 aaaacaggtg aaagctcaga aacgatgttt gctcgacatg cagattttga gtagtagatt
     6121 ttaaagataa aaaaccattc tctaaatgct aattattcag caaatatgag aatggttttt
     6181 ttatttgatt agacataact taataattca gtagttttga gcgtaagcgt gtcatagaat
     6241 ggtacctaaa cacaaatagc ccgatttgat acactcaaat caggttattt gtggcttata
     6301 ttatttacag atttcttgta tctgctgatt ccatggcaaa taagcgcgta gcgcttcagg
     6361 tgtcatcgaa gacagattgg gaatctctgt aaaaagatga tttaaatatt tgcggatgtt
     6421 taacccattc gcaacagctg tctggataat actcaatata attccatttg atctagcgcc
     6481 ttttaagctt ttagaaaata gccagttttt tcgaccgatc actaactctt tgatggcacg
     6541 ctctgctcgg ttatttgaaa gttccaagcg tccatcaagc aacacaatct gaagtccgtc
     6601 tttatgattg actgcgtatt gaaccgcctt tccaagctgc gattttggca aataagcaaa
     6661 actctcacac caatgaaaaa attcagtcat cacaggctgc aacgtttcct tcctcgctac
     6721 aaacctttct tctgcgctaa gcttattaaa ggtccgttcg attcgaaaca tcttgtcaca
     6781 gaagtctaat cctattttgg catgactttt tggggacggt tgtttaccca atgcttcaaa
     6841 aaactttctg cgaacatgcg cccaacaata aacattgttt accggcagct ttccataggc
     6901 cgcgtaacca tcagtatgca agaaaccgct aaatgattct aaaaaacttt ttggctcatc
     6961 aacggaacgc gaatttgcgt gctcgtagag cacaacttgt ttttcttcgc ttttacctga
     7021 agcatataac cagtaaaagg tcttttcttt atgagtattc aatacccgat agcttgtctc
     7081 atccgcgtgt aagacctcct gattgcttaa ttcttgatga aggcaattgt agattggctc
     7141 taaaaccatt tcacttacct tgatatgcca attagtgatt tttttacgat caagagggaa
     7201 accaagtgca tgccattctt tttcttgtcg atatgccggc acctttttaa caaatttttc
     7261 ccaaatactc tgtgtcacaa ttgaagagga acctagacta ttattgatca acggtttagg
     7321 aacaggcgct tttttgattt ttgtttctcc taatgcttca cattctctgc aatgataggc
     7381 atgttggtag atgacttgtt tttctatatg ggcaggaaca tacaatggct cttctctaag
     7441 ccttgtcgca ccgatatcat gtaaagtatg accacagtct tcacacaaac aatcggcacc
     7501 ttttaattca taatgaatag gaacttcagg cagaccttct aatgaaagtg ctttgagtcc
     7561 cttgcgtttt ctgcgtttaa aagaagatac cgtaatgggt tcctggcttt cttgtccagt
     7621 gtgctctggt tcattaaaag ttgagtcgtc taataaatcg atttgcccat ctacgggtgg
     7681 tgtttctttt tttcgtccaa acaagcgtcg attcatttca gcaatttgtt ctctaagaag
     7741 agtattttcc tgttgctgga tttcaagcaa ctgtgttaat tgcttgattt gtacaagaag
     7801 ttcagattcc atttttgtca tttttacacc accattctag cgattttctt acatctatta
     7861 taccatttta tgacaacgaa aaacagacaa aatccttgat ttaacaaggt tttcgtctaa
     7921 tatatcgcac cttttttcgt ttcttttagc gtacattttt cttcgattga aaggccagtc
     7981 agtaagactt cttttctgga acgcggccat tgaaggtacc cactttcaaa tcttttgtac
     8041 attaatacaa atccattttg atcctagtaa agagccttga agcgatcttt cttcgtaccg
     8101 caaaataaga aaagcgcttg atcgtaaaca tcaaggtcat attcttctat aacgaaagct
     8161 gctagtccat caattccttt tcgaagatct gtttttccac aaacgataaa aatatgtttc
     8221 aatgcagttg catcaatgag cattggcatt caactctttt aacaaggtat acaaaagata
     8281 tttatccatt ccattataaa taaatatttc cttatcttcc gatttgattc tgcaagctag
     8341 ttttggcttg gtgtcaattt gcgatttttg agccgcactt gaaaattgaa ctggtacaat
     8401 atctttttta ttcattcgtt tttctccttc tatatttgat agtttaatta tagaagaaag
     8461 ataatcggcg tacgagatac tatcctatga cacgcttaca tttaatttaa tattttgcct
     8521 caaattagcc acacttttta tagaactaag aggaaaaatg aagttttctt cctattaata
     8581 tagttgtttg aaatcttaaa tatagcgttt tacgatgtaa tgggaagtat ggtgaataaa
     8641 tcaactatgt tcgtacgaag catttactca actaatttaa tcgaatcttt caataaacaa
     8701 atcaagaaat acagccaccg caaggaacag ttccaaaatg aagagtcgat ggaacgtttc
     8761 ttagtctcgt cttttgatac ttacaaccaa aaattcctag gtcgcagtca taaaggtttc
     8821 caacaggccg aaggcgagct cgaacaaatg ctgagtcaac cgatggagaa ttagaaacaa
     8881 tctacagtag ggaagaacca ttgacacaaa attattgacg ctcccaacta aaagtgtcaa
     8941 aataatgatg aaatcattta gttcttataa attagaaaag agaaaataaa tattccatat
     9001 ctactttatt ggatccgtaa catgactaaa ttctgaatac tttttaaagc ttatcatttg
     9061 gtaaaactga atataagtta attatataga ctgaaaattt tgaattttca gaatagaata
     9121 acttaaaagt actgacaaaa ttttttaaag gggcaatatt aatgttctga gataatcaaa
     9181 tcctaccgaa aatatcaaaa atccaatgtg acacaatcag attgtgtctc attcgaggga
     9241 atcacacaat cccacgtggc tctaggcaga attgaatttt tgcttggggc tttttcctta
     9301 tattgatttt gaatgagact tattatatgt tataatgcaa ttaagtatca ttggagaggg
     9361 cgaagaaaat gaagacggta gaacctattc gagacaaaaa aaagatcgat gcgatgaagg
     9421 caatcttggc ttcgggaaaa tatggccaac gaaatttggt tcttttttcg attgggatca
     9481 acaccgccta tcgcatttct gatttgcgtc aattgaagct ggccgacgtg ctggaaattt
     9541 cacgcggtcg cgtggtggtc aaagatcgat tagcgatgaa ggaacaaaaa acagctaaac
     9601 ataattcggt ctatatttcc aacaaattgc gaaaaacgat tttggagtac gttcaacagg
     9661 aatttcctga tcaactcctt cagcaagatt ttgacggtta tttgttccct agtcgaaaag
     9721 ggacgaatga accgctcaac cgccaaagtc tgtggcgaat cattcatgat gctggcacgg
     9781 caatcggatt gaaagacatt ggcccacatt ccatgcgaaa aacgttcggt tactttctct
     9841 ataaacaggg gaccaaaaca gaaatcattc agtccttgtt gaaccattct tctcagcgcg
     9901 aaacattgcg gtatattggt attacacaag aagacaaaga tacagctgta aaaagcttag
     9961 atttataata taaaagttaa gtttaaacat ggatagtttt aaattagtag attgagtgtt
    10021 gggaggaaag aatgagtaca ttatatatta taggaaacgg ttttgattta agtcatggtt
    10081 taaaaactag atacactgat ttttcaaaat gggttgaaaa aagtcatagt gaaatattcg
    10141 ataaaataga cacaatgata aatgaaaata atgaggtgag aagtacagct gatgataaga
    10201 taggttggtt taattttgaa agtagtttgc atgagttaat tcaaagtcca gtctttgaag
    10261 attatgtaga agagtctaag gattatctca taggttatgg acatgaagat tggtcagatt
    10321 ctgcccatca tgattttcaa aatatggttt atgattatgt tgtttctcta gaagaattac
    10381 aacaactttt caaggaatgg atacgcaaag aaatacagcc gacaatagaa cataaaattc
    10441 caaaatacca attttcgtcc gatgtactat tctttacatt caattataca gatgttttgg
    10501 aaattctata caagcaaccg aaagaaaaca tatatcatat tcatggcagt tatgacgata
    10561 taataatcgg ccactccgag aattttgaca actttatagg agat
//
LOCUS       DACDEA010000085        10030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDEA010000085 DACDEA010000000
VERSION     DACDEA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.086"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..589)
                     /locus_tag="IV136_002333"
     CDS             complement(<1..589)
                     /locus_tag="IV136_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8466298.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINN"
     gene            complement(582..1700)
                     /locus_tag="IV136_002334"
     CDS             complement(582..1700)
                     /locus_tag="IV136_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8466299.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            complement(1702..2334)
                     /locus_tag="IV136_002335"
     CDS             complement(1702..2334)
                     /locus_tag="IV136_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8466300.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            complement(2345..4162)
                     /locus_tag="IV136_002336"
     CDS             complement(2345..4162)
                     /locus_tag="IV136_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8466301.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4175..4939)
                     /locus_tag="IV136_002337"
     CDS             complement(4175..4939)
                     /locus_tag="IV136_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8466302.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(4967..5665)
                     /locus_tag="IV136_002338"
     CDS             complement(4967..5665)
                     /locus_tag="IV136_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8466303.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5677..6456)
                     /locus_tag="IV136_002339"
     CDS             complement(5677..6456)
                     /locus_tag="IV136_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8466304.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6472..7416)
                     /locus_tag="IV136_002340"
     CDS             complement(6472..7416)
                     /locus_tag="IV136_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8466305.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7462..8241)
                     /locus_tag="IV136_002341"
     CDS             complement(7462..8241)
                     /locus_tag="IV136_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466306.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            8507..9676
                     /locus_tag="IV136_002342"
     CDS             8507..9676
                     /locus_tag="IV136_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8466307.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         2867 a         1969 c         1680 g         3514 t
ORIGIN      
        1 tgttattgat tgtattaata ttctctttct tgattacttt tttacatgtc tgaattataa
       61 taataagatc attaaagaaa ctaattttat caacataata aacatctaaa tcaaactttt
      121 cttcccaact aatcgcattt cttccattga cctgagctag tccagttaat cctggtctta
      181 cgttgtgtct tttccgttgc ttctcactat atagtggcaa atactctact aataatggac
      241 gaggtcctac gatcgacata tcacccttca aaatattcca aagttctggt agctcatcta
      301 gacttgttgc tctaagtatt ttcccaaatt tagttagtcg aatttcatca ggtaaatatt
      361 ctccattgtg gtctctttcg tcagtcattg tacgaaattt atacatggaa aaaatttttt
      421 cactttttcc tggtcgttct tgtttgaaga taattggaga accaagttta attcttacta
      481 aaaaacctac aattaagatt atcggcgata aaacgatgag agcaatgcct gacaatagga
      541 tatctaaaat tcttttccca aaaagctgat agaaagtttt tttacgcatc attgatcact
      601 cttttaatcg tagatactac atattttaaa tcttcatctg tcatctttgt atctgatggt
      661 aaacatactc ctaagtcaaa taaagtttct gctacatcat cgccaatata atcataattt
      721 ttgaaaacag gttgtaaatg cataggtttc catactgggc gggcttctat gttattttta
      781 tctaactcat caataagatg attcgtattt atccccttta ttgttatgac gcttaaccaa
      841 tagttcggtt cattccaatt attttctggc atgaattgta tatttgacaa tttagacaat
      901 tcttttttat aaaaattatg aatatatcgt tttttctcaa ttctttcatt taaaactttc
      961 aattgtcctc gaccgatacc tgcaacgata ttgctcattc gataattata gccaatttca
     1021 ttatgttgat aatggcgtgc cttctcacga gattgtgtag cccaaaatct cactttttcc
     1081 accttttctt tttcattaga cacaagcatc ccgccacctg aagttgtgat tatcttattt
     1141 ccattaaaag aataaattcc atagtcgccg aatgtaccag tatattttcc tttatatgtt
     1201 gttcctaatg attctgcagc atcttcaatt agagtaacat catattcttt gcatatttta
     1261 gtaatttcgt ctaagttagc tgataaaccg tacaaatgta ctactaatac agcctttgtg
     1321 ttaggatatt tttctagcgc tgttcgcaaa tgttcaggtt tcatgttcca agtttcgtaa
     1381 tcactatcaa caaatacagg tatagctccc tgatagagaa ttggattagc agtagctgag
     1441 aacgtcaagg actgacaaat cactatgtca tctttaccga ccccagccgc tttcaaagct
     1501 aaatgtatag ctgctgttcc agaagataat gcggcagcat gactagctcc cacgtattct
     1561 gctaattctt tctcaaatcc attcacattc gcacctaaag gagccaccca atttgtatca
     1621 aatgcttctt ttacatactc ttgttcatat ccttcctcac tcatatgagg agatgctaat
     1681 aatattcgtt tattttccaa cttaatccca tctttctatt actttggtag gtacgccaac
     1741 atttttagta ttgttattaa tattttttat tactacactt cctgcaccta taatattcgt
     1801 atttccaata cttagatttt gtattatttt agtacctact ccaatttcat ttttatctcc
     1861 taatataaca tgtcctgaaa tatttacact tggaaaaact gaattataat taccgatttg
     1921 agtatcatgt ccgatagtag agccgatatt gatcatattg aaatttccta aaactacgtc
     1981 agcagtgtaa gtcgtatagg gcatcaaaat attgccaatt cctaactgaa catttatgcc
     2041 cactagtgca cttggtgcaa ctaaattagg gaaaatcaat ttattatttt tatttatctt
     2101 ctgatatatt ttctctttga tatctggcga agcaattccc agaaaaatag cagtttcttc
     2161 ttctaattct attaacaagt ctattgttcc taatataggt ttttccaata attcttgatt
     2221 ttgcattttt aacgaatcat cgacaaaacc aaccaattca tatctagaga cactttgtaa
     2281 tagaaaagca atctctttgc caaatccacc agctcctaca ataattgctt tcactttact
     2341 ccccctactt atttgaagca tttgcaaaag tgacaatgtc tttagccagt tgttcatcgt
     2401 cttgcggcag gctcttgaca aaatccatca ctttttgtat tgagtaacct ttgatgtttc
     2461 ctacaaagat tttctcataa actgcttcat catttctttc cttatccaac aatagttctt
     2521 catatagttt ttcccctgga cggatacctg tttcaatgat ttcgatttcg tcttcgctat
     2581 acccgctcaa tcggatcatg tttttggcta ggtctacaat cttcactggt tcactcatat
     2641 ctagaataaa aatttcgcca ccttttgcta atgcgccaga ttgaataact aaacgactcg
     2701 cttcaggaat cgtcatgaag tatcgggtca tacgaaagtc tgttacagtg attggaccac
     2761 cttgagcaat ttgttctctg aatactggaa tcacactacc gcgagaacca aggacattcc
     2821 caaatctgac agctgagaat ttcgtacagc cttcttcatt caatccagtc acgatcattt
     2881 cagcgattcg ttttgttgaa cccatgacgt ttggaggatt gttagcttta tctgtcgata
     2941 ccatgacgaa gtttttcact cttgctgctt tggctgcttc tgctacattt tttgtaccga
     3001 aaatattgtt tttgactgct tcttttggat tatattccat cagtggtaca tgtttatgag
     3061 cagctgcatg gtagacgata tctggatgat attgttccat gatctcattg attttctcgc
     3121 gatcttggat atctgcgatg atcggtacga tttctgtagg acaattttga tgatgcgtgc
     3181 gtaactcacg gtcaatcaag tagatcgagt tctccccatg tcctaaaagc aatagctttg
     3241 caggattgaa ttgaatgatt tgccgacaga tttctgaacc gatcgatcct cctgcgccgg
     3301 tgacaagaat gactttgtct gtgatctgat ctttgatgga ttcaatatcc agctttacct
     3361 catctcttcc cagtaaatcc actacgtcaa ttgtttttaa ttttgatacg ctaatttttc
     3421 ctgaagcaag ttcttctata gaaggcatcg tgttgacttt cacacgagcg gattcgacta
     3481 actcgaagat tcttcgtaat ttttttcttg ataaggaagg gatagcgatc gttaccattt
     3541 gaatgtcata tttttcaatc aattccggaa tatcttttaa tgcacctaat actttttttc
     3601 ctgaaagata cgtatttctt ttatttggat catcatctac aaacccgaca acgtggatat
     3661 cttgagccgt tttggatcca agaaagctgt tgtataaaat acgcccgcct tcgccagcac
     3721 cgacaatcaa tgtattttta gcagaatctg tggatagata tctcatattt tttgtttcga
     3781 catagatacg ccagatcaat cggctaccaa taatcaataa tagcgaaagc aggtaagcaa
     3841 aaatgactaa acgtaaactg tatcgccgat ttatgaacag caatatgatg atacttgaga
     3901 cagcacttgc agatagtgag ccaaaaaccg caatgatttc tcttaaattt gtatagcgat
     3961 tgatccgagt gaatactttg aataaactac catagaataa ataaaaacca atcgacaaac
     4021 cgatagagat taaaataagg tccattggta tagctacaaa cggcttcatg aatttagctg
     4081 ctgcaatatt cgcagcaatc aacaacaagc tgtccacaaa taccaatgtg gcaatttttc
     4141 ttcttcttgt taatacaaac accctacatt ccccctaaaa taaaccaaac ttcttctttt
     4201 ttacctcttc atagattgga taactgattt catccccgtt gatcagatcc tttgcgactt
     4261 gtttcatctg tactactttt tccttaccaa agtccttggc aatctgttca tagcactctt
     4321 ttaagtaaaa ggtgcgctta gagaccccat gggcatcaga agcagccatc tgcacaagat
     4381 tatgtttgac catcgttttg gctgttttct ggatgtcttt gccaaatacc ccaacataac
     4441 ttggcgcagt gagttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt
     4501 cttcgcgaaa atgcgcgtta cgttctggat ggacgatcac tggtgtcttt cctagttcta
     4561 acaactcaaa aaaaagccgt tctgcatata acggcacttc cagtgtcgga aattcgatca
     4621 gaagatatgt atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga
     4681 gcgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct
     4741 ctaattcggc ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt
     4801 ttccattatt gtggtgtggt gtgcataaaa tgtgcgtgat gccttgctga atcgcttttt
     4861 cagccattgc aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat
     4921 ggcaatgtag atcgatcatt tctcttgtct cctctcttct tattccttaa tttccgtagt
     4981 aataaccagc atctttgcta tgttctgctc cgttgtacac aacacctaag actcgtgctt
     5041 gtaccatctc cagcagttct tttgctttag tcaaggattc tttacgtgac atattttcac
     5101 gtacgaccaa taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta
     5161 ctactggtgg catatcgaat atcaccacgt caaataactg tcttgcttcg gctagaacct
     5221 gatccattct tggcgagctt aacaattctg atgggttggg tggcttaggt ccactaggca
     5281 aaattgacaa gttctcgact ggcgttcgtt ggatcacatc tgctacactt cctgatgaac
     5341 taagtgctgt acttaatcca ctggcattgt tcagctggaa tgtcttatag acgacgggtt
     5401 tacgtaaatc tgcatcgact agtagaactc gttgacctga tttagcaaaa acgactgcaa
     5461 tatttgctgc agtggtggat ttcccttctc caggacctga tgaagtcacg acaatcgttt
     5521 tgatttgctg accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt
     5581 cggcgattgg tgaagatgga tttgttaatg tgatcaaact gactgcatga gtttgtgctg
     5641 ctttttgttt ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg
     5701 tgatggggct gttttatcgt ttgtagcttt tggtcgtgca ggagctgttc cttttttacg
     5761 gataccagcg ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc
     5821 taatgtatcc gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa
     5881 tgccaaacct acgcctacca tcattcctaa cactaagcca atcactagat tcaatttatt
     5941 atttggcgaa acaggtgtcg tattggcaac tgcacctgaa ataatcgaga ttttatcgac
     6001 attcatcacg tctttggcgt tttcttggaa aacttgtgaa gttgtgttag cgatctgttg
     6061 agcagtcact gcattcgtgc ttattgcctg gatcgagaac atttgtgaat tttgtgactg
     6121 attgacggag atcgcatctt tgatttgtcc agcagtcatt ttcacattgt cttctgtttc
     6181 taaacgatct ttgacttcat ttagtactaa atcactgacg atcatatctt tatacgtatt
     6241 gatcatttgc aggttcgtat tgacatcatt gacatttgct gtgtctgatt gtggcagtgt
     6301 cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaacg tcacgattcc
     6361 tgagatggcc aagccagcaa acattgagat gatgattgtt accaagtgtt tgcgtaatgt
     6421 ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt
     6481 ctctagattc atttgatctt ttaattcttt ttttacacga tcaagttctt gatcactgac
     6541 tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat
     6601 cgtaccaaat gcctcccgat aatctaaagc aatttttttc atttcatcaa agctcatgtc
     6661 tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga caccgtttag
     6721 actcaatact ttttttgcta cgccttctac gatctttctt tgacgctctt gtctaccata
     6781 gtcaccgttt ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa
     6841 agtaatttta ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac
     6901 ttcgatacca ccaactgcat caacaagttc ttttagccct ttcatgttga tagagacata
     6961 gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc
     7021 gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt
     7081 atctcgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat
     7141 catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa
     7201 tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt
     7261 tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc
     7321 tcctactcct acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat
     7381 ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg
     7441 cttgaatcaa cgttttaact gtcaatagct tatattgcta tagcccattt gctgagcaac
     7501 agtttgtgct tttgttgcgt atgctgcgcg tattttttca ttccggatct gctctatttg
     7561 actgagcacc gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catccttcaa
     7621 catcgtatcg aataactgat ctagttgcgt gacttgcgcg atctgagtat cagcgaatcc
     7681 tagatattgc ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg
     7741 tacacgagcg acatctgcac ttgtcacctt ttctttgatg atgacatcgt ctacagcttg
     7801 ctgccagttt gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt
     7861 tgattctgca ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc
     7921 ttctttaggc aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga
     7981 tagcttattt gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt
     8041 ttcttttgta ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg
     8101 attggtgatg gcagattctg cataattgat ccgttgctga tgttgtcgct gattattgat
     8161 atggtaacca aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga
     8221 cttgacgaat ggcttttcca tagattcact cctaagtatt gatgatagat aaattaagat
     8281 ataattcact tacataaaaa tgacagcttt ttattttaaa aaacataatt aaactaatat
     8341 attcacagaa atatttgtaa acaagttttc cctatataat cataccacaa actgttttga
     8401 tagcgtaaaa tattttgtgt aaatagatat ttagggtaga aaaaaagaaa gtccattctg
     8461 taaaattaaa gttaccacaa ccgaaatttt ataggaggac tttctcatgc ccaattttac
     8521 tacagaaatt atggaaacac taatcaataa aggtgattta gatgaattat ttcgatgtca
     8581 tttagaatta gctgttaatt cattattgca ggctgaatta acagcttttc ttgattatga
     8641 aaaatatgat cgtgctgggt tcaattcagg taattcccgt aatgggaatt attcacgttc
     8701 attcagaaca gagtatggag aattaaattt ggtgattcct agagatagaa atggtaagtt
     8761 ttctcaacaa actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat
     8821 tcagctattt caaaaaggaa ttactatgtc tgaaatctct gaattgattg aaaaaatgta
     8881 cgggcatcac tatacgccac aaaccatttc aaatatgact aaaatagttt ctgaagatat
     8941 tattgccttt aaagaaaggt ccttagaatc cagatactct gtcattttta tggatgctac
     9001 tcacattcct ttaaaaagac aaactgtttc aaaagaagca gtttatattg taattggcat
     9061 tcgattagat ggaaccaaag aggttcttgg gtttagtctt gctccaactg aatctgctta
     9121 tgtttggaaa gagattcttc aagacctaaa agaccgtggt ttaaaagagg ttttattagt
     9181 cgtaactgat ggtttaagtg gcattaacga tagtattcat agtgtttatc caaatgctca
     9241 atttcaacaa tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga
     9301 tcgacaagaa atctgtagcg atttcaaatt agtttatcaa gcaagttcaa aagaagaagc
     9361 aaataatcaa ataaggttta tgatagataa atggaaaaag cagtatccac gagtggttaa
     9421 attgctcatg aatcctgcta tattaacctt ttataatttc cctccgtcaa tcagaagaac
     9481 tatttattca actaatttga ttgaaggttt taacaaacaa ttaaagaaat ataccaaaag
     9541 aaaagagcaa tttcctaatg aagaatctct ggagagattt ctagtatctc aattcaacaa
     9601 ctataatcaa aagtttctat gccgagttca taaaggtttt aaagaaatac atgatacatt
     9661 agaatcaatg ttttaacttg atcaacagaa tgggcttttc catttacaca aaattattga
     9721 cgctcccgac aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat
     9781 taatgaaaag cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg
     9841 ggagttctta gtatattgtt caaacttaaa tttcatataa tttaattatg taaattagct
     9901 attctaagaa cttctacgga taactgcccg tctaaaattt aaataagagc ttagaatctt
     9961 catataagta aggttaattt ttttgtacaa aaaagtcgga atattctgag ttactgaaat
    10021 gatataaaat
//
LOCUS       DACDEA010000086         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDEA010000086 DACDEA010000000
VERSION     DACDEA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.087"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV136_002343"
     tRNA            8..81
                     /locus_tag="IV136_002343"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV136_002344"
     tRNA            101..173
                     /locus_tag="IV136_002344"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV136_002345"
     tRNA            201..274
                     /locus_tag="IV136_002345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV136_002346"
     tRNA            288..361
                     /locus_tag="IV136_002346"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV136_002347"
     tRNA            385..474
                     /locus_tag="IV136_002347"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV136_002348"
     tRNA            487..560
                     /locus_tag="IV136_002348"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV136_002349"
     tRNA            579..654
                     /locus_tag="IV136_002349"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV136_002350"
     tRNA            656..726
                     /locus_tag="IV136_002350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV136_002351"
     tRNA            740..813
                     /locus_tag="IV136_002351"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV136_002352"
     tRNA            838..925
                     /locus_tag="IV136_002352"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV136_002353"
     tRNA            947..1018
                     /locus_tag="IV136_002353"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV136_002354"
     tRNA            1041..1130
                     /locus_tag="IV136_002354"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV136_002355"
     tRNA            1143..1216
                     /locus_tag="IV136_002355"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV136_002356"
     tRNA            1235..1307
                     /locus_tag="IV136_002356"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV136_002357"
     tRNA            1311..1383
                     /locus_tag="IV136_002357"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV136_002358"
     tRNA            1388..1458
                     /locus_tag="IV136_002358"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV136_002359"
     tRNA            1471..1544
                     /locus_tag="IV136_002359"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV136_002360"
     tRNA            1556..1627
                     /locus_tag="IV136_002360"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV136_002361"
     CDS             1893..3086
                     /locus_tag="IV136_002361"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8466308.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV136_002362"
     CDS             3216..4688
                     /locus_tag="IV136_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8466309.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV136_002363"
     CDS             4771..5559
                     /locus_tag="IV136_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8466310.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV136_002364"
     CDS             complement(5626..6294)
                     /locus_tag="IV136_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8466311.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV136_002365"
     CDS             6409..8121
                     /locus_tag="IV136_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466312.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IV136_002366"
     CDS             8124..9896
                     /locus_tag="IV136_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466313.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACDEA010000087         9815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDEA010000087 DACDEA010000000
VERSION     DACDEA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.088"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            205..684
                     /locus_tag="IV136_002367"
     CDS             205..684
                     /locus_tag="IV136_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466314.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="IV136_002368"
     CDS             717..2945
                     /locus_tag="IV136_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8466315.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="IV136_002369"
     CDS             3074..4717
                     /locus_tag="IV136_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8466316.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="IV136_002370"
     CDS             complement(4790..5335)
                     /locus_tag="IV136_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8466317.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="IV136_002371"
     CDS             complement(5358..5843)
                     /locus_tag="IV136_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8466318.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="IV136_002372"
     CDS             complement(5833..6645)
                     /locus_tag="IV136_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8466319.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="IV136_002373"
     CDS             complement(6784..8316)
                     /locus_tag="IV136_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8466320.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="IV136_002374"
     CDS             complement(8425..9456)
                     /locus_tag="IV136_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8466321.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3262 a         1827 c         1892 g         2834 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taatt
//
LOCUS       DACDEA010000088         9618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDEA010000088 DACDEA010000000
VERSION     DACDEA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.089"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            145..351
                     /locus_tag="IV136_002375"
     CDS             145..351
                     /locus_tag="IV136_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8466322.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(553..1554)
                     /locus_tag="IV136_002376"
     CDS             complement(553..1554)
                     /locus_tag="IV136_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8466323.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1559..3472)
                     /locus_tag="IV136_002377"
     CDS             complement(1559..3472)
                     /locus_tag="IV136_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8466324.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3640..4146)
                     /locus_tag="IV136_002378"
     CDS             complement(3640..4146)
                     /locus_tag="IV136_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8466325.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4306..4746
                     /locus_tag="IV136_002379"
     CDS             4306..4746
                     /locus_tag="IV136_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8466326.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4772..5929)
                     /locus_tag="IV136_002380"
     CDS             complement(4772..5929)
                     /locus_tag="IV136_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466327.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5932..6288)
                     /locus_tag="IV136_002381"
     CDS             complement(5932..6288)
                     /locus_tag="IV136_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466328.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6586..7560)
                     /gene="sgrA"
                     /locus_tag="IV136_002382"
     CDS             complement(6586..7560)
                     /gene="sgrA"
                     /locus_tag="IV136_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8466329.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7756..8595)
                     /locus_tag="IV136_002383"
     CDS             complement(7756..8595)
                     /locus_tag="IV136_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8466330.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8780..>9618
                     /locus_tag="IV136_002384"
     CDS             8780..>9618
                     /locus_tag="IV136_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8466331.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         2887 a         1942 c         1447 g         3342 t
ORIGIN      
        1 ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc tagatgatca
       61 ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat ttatcataca
      121 attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct ttttttcgct
      181 agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa taacaacgga
      241 atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct tcttgcctcc
      301 gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg acttataaaa
      361 tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg aataaaaggt
      421 gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac catgaaaagc
      481 ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca acctcttttg
      541 catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt cttgcgcttg
      601 tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct ccgcataatc
      661 tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt ccatttgttt
      721 cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac gatccgcatg
      781 tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat tatcaaagta
      841 agcccatcca catttttcat aaagaaagct agagatttta tcacttactt gagtaagtgc
      901 agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt tctccaaagt
      961 cggatcaaaa tgatcagctt ttttaatttc ttcattttga agtgctaaca aatattctcc
     1021 actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat ctgcttgatc
     1081 ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg gtgtaggatg
     1141 ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg tgtctgaaca
     1201 tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg cgcctccatg
     1261 catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga tatccatatt
     1321 attatttttg tactttgaag gataggatat ttcttttttt atatcgattt gttcgatttt
     1381 ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt caaattgttt
     1441 tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata cagtgactaa
     1501 aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt tcattcattt
     1561 accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa aatccacctg
     1621 aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt ttttttgcat
     1681 ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca cttgattgat
     1741 cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct tgcttttttg
     1801 gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc tcattaaaaa
     1861 tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc ttttgctctg
     1921 tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct ttgagtagac
     1981 ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact actttcaaaa
     2041 agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt ccatgccctg
     2101 ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat ttttgcgcgt
     2161 tagacacagg tactacatca tctgctgttc catggataat caatttaggc aatgaatctg
     2221 acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga tcaaaatagt
     2281 attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg atgtctaact
     2341 gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta aaacgtagtt
     2401 gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg acaggaatat
     2461 ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa agtaaaatct
     2521 tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca tctgactcat
     2581 attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa tcaacaaata
     2641 ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc ccgttattcc
     2701 aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga tcgacataat
     2761 atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga tgaagttgtt
     2821 ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct gtacctccaa
     2881 gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga taagtagcaa
     2941 tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc tcagtgacta
     3001 ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt tctgtcatct
     3061 gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta ccgatatcat
     3121 cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg tcgtttgaca
     3181 tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc gtttcttgat
     3241 ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt tgtgaagtac
     3301 tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc cgagattccg
     3361 attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta cttacagcaa
     3421 ataagatgac caacgctccc tttaatactt gttttgttct caagctattc actccttttt
     3481 atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa taaatggttt
     3541 atcccataaa aatagaacag gattattttc tcattttacg taataaagca gaatggataa
     3601 accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca aaaatgtcag
     3661 aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt tcataaaagg
     3721 attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta atccatgagc
     3781 aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt cgatcgaatg
     3841 acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat tcgcatgggt
     3901 ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct cccatggttt
     3961 tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt tcccaatcgt
     4021 ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt cgtgtatcgg
     4081 cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt cggctgtgtt
     4141 tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta tctagctagt
     4201 tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt ttcgactatt
     4261 tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat gattaaattg
     4321 tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat cgtccatgat
     4381 ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga atttatccac
     4441 ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc tattagttat
     4501 aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat tgagattcgt
     4561 acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa acttttgact
     4621 ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa gcaagagctc
     4681 aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct ttttgcaatg
     4741 tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat ttttccaata
     4801 tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc gcccaatcat
     4861 tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca tatgcctttt
     4921 tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat gcactatatt
     4981 ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc tgtgttttac
     5041 catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa atatccctga
     5101 ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta ttaagctctg
     5161 gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc actggaaata
     5221 tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc ttttttgtcc
     5281 caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa tttttttctg
     5341 aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac gattcaatcc
     5401 agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc aaataggtgt
     5461 tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg tgattcatcg
     5521 accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct tcttttgtta
     5581 tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag tggccttgtt
     5641 tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct atactacgcg
     5701 tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt atacttgata
     5761 atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa aagtatcgga
     5821 tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat aaatgatagt
     5881 ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa cttatataat
     5941 tttttgacta agattttcta aggattgata agtacctttg taaagtgaat ccagtaaaat
     6001 ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga
     6061 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
     6121 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
     6181 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
     6241 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
     6301 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
     6361 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
     6421 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
     6481 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
     6541 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
     6601 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
     6661 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
     6721 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
     6781 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
     6841 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
     6901 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     6961 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     7021 gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     7081 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     7141 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     7201 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     7261 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     7321 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     7381 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     7441 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     7501 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     7561 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     7621 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     7681 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     7741 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     7801 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     7861 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     7921 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccgatt
     7981 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     8041 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     8101 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     8161 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     8221 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     8281 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     8341 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     8401 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     8461 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     8521 attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc caatcaaccg
     8581 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     8641 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     8701 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     8761 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     8821 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
     8881 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
     8941 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
     9001 agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc
     9061 aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt
     9121 taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga
     9181 tctagacact gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc
     9241 gactaaagaa ggagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa
     9301 gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt
     9361 tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa
     9421 cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta
     9481 tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga
     9541 gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat
     9601 ccgtaaagtg atgaaaaa
//
LOCUS       DACDEA010000089         9265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDEA010000089 DACDEA010000000
VERSION     DACDEA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.090"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IV136_002385"
     CDS             51..260
                     /locus_tag="IV136_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466332.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IV136_002386"
                     /pseudo
     CDS             467..667
                     /locus_tag="IV136_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IV136_002387"
     CDS             complement(1379..1984)
                     /locus_tag="IV136_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466333.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IV136_002388"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IV136_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IV136_002389"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IV136_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IV136_002390"
     CDS             3019..3990
                     /locus_tag="IV136_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8466334.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IV136_002391"
     CDS             4130..5443
                     /locus_tag="IV136_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8466335.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IV136_002392"
     CDS             5486..6352
                     /locus_tag="IV136_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8466336.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IV136_002393"
     CDS             6349..7197
                     /locus_tag="IV136_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8466337.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IV136_002394"
     CDS             7211..8683
                     /locus_tag="IV136_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8466338.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>9265
                     /locus_tag="IV136_002395"
     CDS             8700..>9265
                     /locus_tag="IV136_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8466339.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIE"
BASE COUNT         2909 a         1583 c         1921 g         2852 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaa
//
LOCUS       DACDEA010000090         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDEA010000090 DACDEA010000000
VERSION     DACDEA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.091"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV136_002396"
     CDS             complement(<1..173)
                     /locus_tag="IV136_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8466340.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV136_002397"
     CDS             complement(593..1714)
                     /locus_tag="IV136_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8466341.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IV136_002398"
     CDS             2042..3451
                     /locus_tag="IV136_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8466342.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV136_002399"
     CDS             3448..4176
                     /locus_tag="IV136_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8466343.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV136_002400"
     CDS             complement(4304..5215)
                     /locus_tag="IV136_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8466344.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV136_002401"
     CDS             complement(5232..6239)
                     /locus_tag="IV136_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8466345.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV136_002402"
     CDS             complement(6236..8365)
                     /locus_tag="IV136_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8466346.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV136_002403"
     CDS             complement(8370..8477)
                     /locus_tag="IV136_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466347.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACDEA010000091         8458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDEA010000091 DACDEA010000000
VERSION     DACDEA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.092"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(147..824)
                     /gene="nth"
                     /locus_tag="IV136_002404"
     CDS             complement(147..824)
                     /gene="nth"
                     /locus_tag="IV136_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8466348.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(831..1529)
                     /locus_tag="IV136_002405"
     CDS             complement(831..1529)
                     /locus_tag="IV136_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8466349.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1797..2981
                     /locus_tag="IV136_002406"
     CDS             1797..2981
                     /locus_tag="IV136_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8466350.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2987..3430)
                     /locus_tag="IV136_002407"
     CDS             complement(2987..3430)
                     /locus_tag="IV136_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8466351.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3656..3943)
                     /locus_tag="IV136_002408"
     CDS             complement(3656..3943)
                     /locus_tag="IV136_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466352.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4114..5466)
                     /locus_tag="IV136_002409"
     CDS             complement(4114..5466)
                     /locus_tag="IV136_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8466353.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5739..6185)
                     /locus_tag="IV136_002410"
     CDS             complement(5739..6185)
                     /locus_tag="IV136_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8466354.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6421..7347)
                     /gene="cysK"
                     /locus_tag="IV136_002411"
     CDS             complement(6421..7347)
                     /gene="cysK"
                     /locus_tag="IV136_002411"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8466355.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7487..8374)
                     /locus_tag="IV136_002412"
     CDS             complement(7487..8374)
                     /locus_tag="IV136_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8466356.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2297 a         1741 c         1408 g         3012 t
ORIGIN      
        1 ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt acatttactt
       61 tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat aattcataca
      121 tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag cgccttttct
      181 tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca tacctcgcac
      241 ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg atgtgtcttt
      301 acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat gaccgtagca
      361 tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc tactgcgatt
      421 gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc tacaccagga
      481 agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc aatcagctgc
      541 tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt ttttatttta
      601 agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa agaggcaaac
      661 aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa gatcactgca
      721 atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg aaacattcca
      781 tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg ctactcctcc
      841 aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg ttctacttct
      901 ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg ttttgtacgt
      961 agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact ataagcctga
     1021 tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg gtcttcttct
     1081 agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa ttcttgttca
     1141 aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta ttttttttct
     1201 tgtgttttta aatactcacc taatagatca tagatggggt aaagattata tcgatctgct
     1261 tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac gagacgatcc
     1321 agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat ctgcaaatca
     1381 gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa aaattcttca
     1441 gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac tgtttgtcca
     1501 gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca cagtatacgt
     1561 aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg atcggtcaga
     1621 tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta tcttcacttt
     1681 tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa ggaaacaaaa
     1741 ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt ttttacatgt
     1801 acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta ttcactggat
     1861 taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat tctcactcca
     1921 tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta gctgccttct
     1981 tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg atccttatta
     2041 tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat tcgatatgga
     2101 tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc accttatatg
     2161 cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga tggatgggtg
     2221 ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta gctgagttgg
     2281 ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc gtttatggct
     2341 acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta agtttctccc
     2401 atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt attcctttat
     2461 tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc ttttctcaat
     2521 tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc tctttgattg
     2581 gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa aaattttcag
     2641 aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta ttcatcacat
     2701 tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt tatggtttcg
     2761 gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc tcagataacc
     2821 agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc
     2881 cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta ttcggatttt
     2941 tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat ttgctacttc
     3001 gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct tcttttgaca
     3061 tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc tcgtgtctat
     3121 tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc
     3181 tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta ctgatggctc
     3241 ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg attttcccga
     3301 tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt tccatttctg
     3361 gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt agcagttgtc
     3421 ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt ttcctattca
     3481 acaaagataa aaatattttg taggaaaacc caatctgaaa ccaagaaaaa aaggggttga
     3541 caaagcttgt cataacccct ttttctgatt aaaagatagt aaagagacag tcatcggtaa
     3601 ttgtgatgat tttttgtcat cacttaagga tgagcggcct tttcaaaaac tgtctttaaa
     3661 gatcatcatc agtttctagc acatatggct gaccttcata tgtgtactct gtattcaatg
     3721 tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg aaaacgatga gaaacattat
     3781 ctttggcgtc atttgctggc atcgtgtaat attcctcttg cgtttcattc aactgagaga
     3841 gtacttgatc tgccaactca tcaatattga tcgttaattt cttcgaagca cttttataat
     3901 cttctaaaaa tgcatcttgt tggacaaaat attctttatt catcttcttt ctcacctttc
     3961 ttttcaaaat caaataaagc tgcttgttct tttatgtaac agctttatct ttgctgctta
     4021 ttgtgacaaa aaattcgaag aaagacaact aattgtactt ttcacaaaaa aagacagagg
     4081 gtcgtgacaa aattgtgtca caaccctttt tctttattgt gcagtttcga tttcgcctag
     4141 cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg tcaaaatgtg gttggaagaa
     4201 gaaatctgaa agtgcgagat cttctacagt cattttattt tgaacagcta aagacatggt
     4261 atttgctgat tgtgtaatgt cgtatttgga catgaactga gcacctacga tacgattcgt
     4321 tcctttttca tagactagct ccatcattac tttttcagta gttggcatga actctggacg
     4381 ataattatct tcaaatacag tcgcttggac ttctagatcg ttcaaagttg cgctttcttt
     4441 ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa agtcctgaag ttccttgagt
     4501 accacgatac gccattttat gatctgtcaa gttatttccc acaagcaaac cttgacgaac
     4561 ggcatttgtt gccaaaggaa tatagttttg cgtgccactt ggattgtagt tgactactgc
     4621 agagtctcca gctgcaaaaa tgtctggatt acttgtctgc atgtattcat tcactttgat
     4681 tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt tttgtatttg gacggaagcc
     4741 cacacataaa atgaccatat ctgtatcaaa tgtatctgtt ggtgtagcta cttgtttcac
     4801 ttttccttct tcatctgtga taaattcagt aacattttcg cctagcgcaa gagtaacacc
     4861 gcggtctgtc aattcttttt ctaagatatc tgtgaatggt ttatctagat atttattcaa
     4921 gatgcgatct aatccatcaa tcaatgtcac ttgttttcca gattcgacaa aagcttctac
     4981 taattcaata ccgatatatc caccaccaac aattactaca cgttttgctt ctttcgcttg
     5041 tgcaataatt tcatttgctt gattgtagtt tttgcatagg agtacatttt tcgcatcgat
     5101 tccactaatc ggtgggataa taggccatga accagttgtc atcactaatt tatcataaga
     5161 aaccgtttct gtttctcctg ttttcagatc ttttgccgtt actgttttat tttctgtatc
     5221 gacgtctgtt acatcgtgtt ccattttaac tgttgctcct aatgaagata attcttcagg
     5281 atttgagtag aacaagcctg ctgggtcctt tactactccg ccgacataaa gtgcgatacc
     5341 gcaagataag aaagaaatat tgtcatttct ttcatatact gtcacttctg cttctggatg
     5401 attttttaaa atacttttta cagctgcagt acctgcatga gtacagccaa ttactactac
     5461 tttcatttat ggtccctccg attaacattt tgtccttcga caaaactact ataacagaaa
     5521 taaaacggat acaacagttt aatgcttgtg aattcacaaa ggtttgtttg tagtttcatt
     5581 ccttttcaag cataagaacc caatatctac aagattctta ttttaataat attcactttt
     5641 atatctaaga ttgtgaataa aagacaaaaa aataaaaaag ctagcgtttt ttttacacag
     5701 ctagcttttt ttaggtataa cacttgtaga aactgttttt acatttgcgc cttttttgct
     5761 gactctttac aatccggaca gagtccatac aattccaacc gatgttcatt aatctcaaaa
     5821 tcagtcagtc tgcttgcagc catttctata tcttcaagcc ctgggtaatg aaaatcaaca
     5881 atcttaccgc agttttcaca aattacatga taatgacgct ttgaactgaa atcaaatcgg
     5941 ctagaagcat cgccgtactt catttcttga acaaatccaa tcgaagtaaa taagcgaagg
     6001 ttattgtaaa cagtcgcaac actcatattt ggaaaccgat gttcaagtgc tttataaatc
     6061 tcatcagccg ttggatggga atgatgttca atcaaatatt ctaaaaccgc gtaccgttga
     6121 ggtgtgattc gaatgttctc tttttttaac ttttcgataa cctcttcgac taacttattt
     6181 tccatgtaaa caccccacgt tgttattctc gtcttatcat actattttta cattttaaaa
     6241 tccacttaac atgaaaagaa aattcaattt tattgtattt taatgaaaaa agacgtaaaa
     6301 cagtcgcgtt cactattcta cgtctctatt caaataattt ttctcatcat aaatttttgt
     6361 gtgttctttt catctttaaa agttttttac ataaacctat ctggagaaaa accaagtgcc
     6421 ttatatctct tggtaaaggg ctgtagataa ataacgttca ccattgtcgg gaaccacagc
     6481 taataccttc ttgccttttc ctaattcttt tgcgactttt agcgcggcag caattgcagc
     6541 gccagatgag atgcctacta aaatcccttc ttttcgccca acttctctag ctgtttccat
     6601 cgcttcgtct ccactaatag aaaggacttt atcatacaca gaagtatcca gtgttttagg
     6661 aacaaaacct gtaccgattc cttggatttt gtgaggtccc gcttctttcc cttctaaaat
     6721 agcagattct gctggttcta ccccgattaa ttcgattttc ggatacacac gttttaattc
     6781 tcggccagcg ccagtgatcg ttcctcctgt accgatacct gcaacaaacg cgtccaagcc
     6841 atttactcca aatgcttgat gtatctcagg accagtcgtc ttctcatgca caagaggatt
     6901 tgcttcattt tcaaattgta atggtaaaaa gtagccgttt tcttttgcca aacgttctgc
     6961 ttctctgatt gatccagaaa taccatccgc accaggagtc aaaatcaact ctgcgccgta
     7021 ccctttcatc aataaacgtc tttcgatact catcgtctca ggcatcacga tgattacttt
     7081 atatcctttt gcaactccta ccatcgacaa tccgattcct gtatttccag aagtaggttc
     7141 aatgatagta tcacctggtt tcagtagacc atcgtgttct gctttttcta tcatacttaa
     7201 agcaatacga tcttttacac ttcctcctgg attaaaaaac tcaagcttta caaaaacatc
     7261 tgctgaatct tctggtacga ttttatttaa tttgacgatt ggcgttttgc caatcaattc
     7321 ggtgactgag ttatagatag ctgtcatctt caattcctcc ttgtctttgc tcttcttact
     7381 tatcataata caatctgttc atttatggcg agtgattcgt ctttgactag caaaaaaaag
     7441 acaaagatat accatatatg gtaatttctt tgccttttta tctaaatcaa acgtttagtt
     7501 cttgaccaac taataaagaa taactggaca tagacatgcc atttttcgcc attagatcat
     7561 caacagacat gccaaatttt tgtgcaatat cccatagcgt atcacctgac acgactgtat
     7621 atgtctgacc attatttgta tttacatctg ttgttgaaga tgatgacgtt gaagtcgtcg
     7681 tagtagtcgt tgttgttgta cttgtggttg ttgtagccga acctgttgat ggcgtatcaa
     7741 aacgggttaa attataagta ttaatcaatg agttcaattt ggccccataa tttggatcgg
     7801 tagcataacg acctgtcaag tacgctgttg catcataaaa acttgttgta ttgcttttcc
     7861 acacgccagc ataatgataa tcgcccgtgg aaaaactggt tgaacgaaga acattcgcat
     7921 ggtcttggaa ggattcccaa taagaagaat attttcggaa tggctcattc atcgttaccc
     7981 attgtccgtt tacatattct ttggttgcca tataaacgac ctgtccgtta tagcttcctt
     8041 tcacaccgaa tagattataa tttggcgcac tagctaatcc ggaagtgcca tatgcacttt
     8101 ccaacaacgc ttgagcgatc atgactgaag catacaagtc attacttgca gcaacttctg
     8161 acgcagacca gccgatttga tcgataaacg cttgaggatc agcgactgtt tgagatcctt
     8221 gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat catcggccca actaaaagac
     8281 tcgttccaat taaaggagca atcgcttttt tattgatcac catttttctt ttaggtctat
     8341 ggtgccgggc actgcgggtc ttcagctctt gcattcaaat tcctcctcaa aataaaaaaa
     8401 cttaataaaa agttctcttc ttaaaacaaa cttaattaat taaattatac ttttaatt
//
LOCUS       DACDEA010000092         8294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDEA010000092 DACDEA010000000
VERSION     DACDEA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.093"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..433
                     /locus_tag="IV136_002413"
     CDS             11..433
                     /locus_tag="IV136_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8466357.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(599..1792)
                     /locus_tag="IV136_002414"
     CDS             complement(599..1792)
                     /locus_tag="IV136_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8466358.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2016..2393
                     /locus_tag="IV136_002415"
     CDS             2016..2393
                     /locus_tag="IV136_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8466359.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2381..2719
                     /locus_tag="IV136_002416"
     CDS             2381..2719
                     /locus_tag="IV136_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8466360.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2853..3296
                     /locus_tag="IV136_002417"
     CDS             2853..3296
                     /locus_tag="IV136_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8466361.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3558..4373
                     /locus_tag="IV136_002418"
     CDS             3558..4373
                     /locus_tag="IV136_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8466362.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4383..5045
                     /locus_tag="IV136_002419"
     CDS             4383..5045
                     /locus_tag="IV136_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8466363.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5250..6914
                     /gene="alsS"
                     /locus_tag="IV136_002420"
     CDS             5250..6914
                     /gene="alsS"
                     /locus_tag="IV136_002420"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8466364.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6927..7637
                     /gene="budA"
                     /locus_tag="IV136_002421"
     CDS             6927..7637
                     /gene="budA"
                     /locus_tag="IV136_002421"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8466365.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7767..8264
                     /locus_tag="IV136_002422"
     CDS             7767..8264
                     /locus_tag="IV136_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8466366.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         2786 a         1405 c         1677 g         2426 t
ORIGIN      
        1 aggagaaaat atgcagactt ttttaacaaa agtttctcaa aagaaaatta aaatgattca
       61 tttactgctt gaaacagaac gttggtgttc aatagaagaa ctacaaaacg aattgaaggt
      121 atcaagtaag tctatcttga attatctaac agatttagaa gaattgttcc aaaaatatcc
      181 tgataaggtc attttgaaga atgagaataa tcggcgcttt tctatggaaa agcaggagaa
      241 cttcccaatc tacattattt atcttcattt ctacagaaaa tcatataatt atcatttgat
      301 tgaattcatg tatcaacatc ctgagaaaaa tttggaagat tatgcagacg ctcaatatac
      361 gagtgtatcc acagtttttc gttatgctaa gttattagta aagtactttg aaatgacgga
      421 cgtcaaagaa tagtcgccgc cagatagaag gaaattagca gttgaaaaag ctgccctgca
      481 tcaagtaata cgaccagcca aacggaaaca gtgtctcatg tttttgattt ggctggtctt
      541 attaccgcag atgcaagctt ttgccacacc tctttcttgt catttacaga ctcaactatc
      601 aatgctgcaa gctttccctt aatgctgtaa caccagtaag agttgtcaag accagcagac
      661 tgactaggta aataccgctc aaaaacaaga ttacggcagt taaactataa tgatccatca
      721 agaaaccgat gacgatagaa gaaaaaccac caacagctcg tccaaggttc ataatgaaat
      781 tattcgccgt cgcgcggatt tctgttggat acaaactact aatcactgcg ccatatccac
      841 catacatgcc attgataaag tagccgacta tcacagcggc tgctaccaaa ctccactggc
      901 tatgggctaa cacaagtaga tagacagata aggatgaaca aattaaaaac aaagaaaagg
      961 ctagtttagg ccccaattta tccatgatta tgccaaaagt catcatcccc aaagacatgc
     1021 ctaaaatagt actgaccatc cacaatgaag acccagaaac cgaaagccct aatcgatttt
     1081 gcataattga aggcagccaa ttcattaacc caaaatagcc ggcaatttgg acagttacca
     1141 tgatactaag gccacatgtt tgccaagcaa gatgttttga ttcaaacagt ctgctaagtt
     1201 tggttttctt ttcggcagat tgtacagatt tgaattcctt tggttcagtc aagccgcgac
     1261 gaatgaacaa gaccatcaac accggcagta cacctacgac atacaacatc ttccaaccaa
     1321 acgttggaat gatcacagca gctaaaatcg cagccaaagc cgcaccgacc tgcgcaccaa
     1381 taccagcaac cgaagaagct cgtccaattt gtttttttga aaaactttcg gaaatcaagg
     1441 acatacacgc cccgtattct cctcccgctc ctacacctgc gataaagcga aagagataaa
     1501 ccaagtagat gtttgaagca aaatacatca ataaagaagc aatcgaaaac agaaggaccg
     1561 tttgagaaaa tactttcaca cgtccatatt tatcagctat gatgccaaaa aaaatacctc
     1621 caatgagcat ccccaaatta gtgatcgttg aaatgaaacc cgcttgcgta ccactaatat
     1681 gaaaagaagt aatcattgaa gataatgaaa aagctaaaaa cataatatcc atattctcta
     1741 aagcaattcc cgccgatgtc gataataata cctttcgctg atacgttgtc atttttatac
     1801 cccttttcct aaaatttccc taaattgtta aaagtatatc caaaaaagaa taaaaaaaca
     1861 agactttttt atttgaaata atacacaaac aaccgatata gataatccag cttagtttta
     1921 atagaaatga gtattgactt ggagtacact taaagttgta gaataaataa aatacataag
     1981 taaagtggca agaaattaag agaaagagtg atcagatgaa tatcaaacaa gtcagtgaag
     2041 aaaagggcat ttcggccgat acattgcgct attatgaacg aatcgggttg attccaccag
     2101 ttaatcggac aaaaggcgga atccgtgatt atacagaaga agacctaaaa tgggtcgact
     2161 ttacgatctg tatgcgaaat gccggattat cgatcgagtc tttgattgaa tatattcgcc
     2221 tatctagcga aggagaagca actgtatttg agcggcgtaa gcttttgatt gaagaaagtg
     2281 aacttttgac gaaagaaatt gctgaaatgc aagagtgttt ggaacgttta caaggaaaaa
     2341 tcaagaaata tgatcgtgtg ttatctagaa ataaaatgga atgtatcaag taaaaaaatc
     2401 aaggagcatc gctatggagt tacgcttact aaaatatttt tggacaattg cagaagaagg
     2461 aacaatctct caagccgcag aagttctaca tcttacacaa ccaaccttaa gccgacaact
     2521 gaaggagtta gaagaggaac taggaacaga actgtttgta cgcgaaaacc gcaaaatgat
     2581 cttgacagaa gcaggttatt ttctaaaaaa gccgagcaga agaaatttta gatttaacga
     2641 atcaaaccac tcaagaattc gagaacagaa aaaaacaatt attcagcggc cgatttacga
     2701 tcggctgtgt ggaagctgat aattcgaata cattggcact attactagaa aagttgatcc
     2761 gtgattatcc cagcgttcaa tttcatatcc acagcggaac aagtgatgat atcacggagt
     2821 gactagaaaa aagcctgtta gatttagcga ttttgctaga tccagtagat acagagaaat
     2881 acgaaacgat tttgttacca agaaaggaaa aatggggatt gctcatttta gaaaattctg
     2941 ttttagcaaa aaaagacgtg ataacaaaag aagactttgc gaatgtacct cttttgatct
     3001 caaagcgtcc agaaatacag cgaatgtttg ctgactggtt aggcataaaa aaggagcagt
     3061 tgctgatcac aggaacgtac aatttgattt tcaatgtatt tgcgctaatt gagaatcagg
     3121 taggtgctgc tttagtcata gaaggtgctg taagtcatct ttcaaagcga gtgacattcg
     3181 ttccgcttga cccaatgatt gaaacgaact gcatgctagt ctggaaaaag aatcggattt
     3241 tatcacctgt agtcaatgaa ttgattcacc gtttcaaaga agcttttgaa cagtaataag
     3301 tatctctaat cgaagaaatt tcaatacaaa agcgagacgt atgacagtct tgcttttgta
     3361 ttttttcatt tttaaatcaa aaaaaataaa gagatttaca taacttgtta aataagcgct
     3421 tacaaacata agaagaacgg aaatgaatca gctataataa ccgttgttct ggattgtaat
     3481 cattacaaag aaatgaagat tataactttg gaggtgttcg aaatgaaaga aaaaaagcaa
     3541 aaattcgttc aaaaaacatg ccctttattc aatggttggg tatgattctg ctgattgtat
     3601 tagctttatt gacgatcatt ccttttttat ggatggtttc tgcttcattc aaagcaaata
     3661 atgaagtgtt cacgattccg attcaatgga tccctaaaac ttggcattta gagaattatc
     3721 tggttatttg gtctcgtatt cctttagtga cattttttaa gaatacagct tttttaagcg
     3781 ttgtgatcac gttgattcag ttgtttactt ctagttttgc tgcttacggg ttttctaaac
     3841 taaattttaa agggcgcgat attttatttt tagcatatat cggcacgatt gctgtccctt
     3901 ggcagtctta tatgatcccg cagttcatta tgatgagaaa agtgggttta acatatacgt
     3961 tgtggtcgtt ggtgctatta caagcgttca atgcttttgg tgtgttcttg ttgaagcaat
     4021 actacagtag tatcccagat tctttatgtg agagtgcgcg gatcgatggc ttatcagagt
     4081 ggggaatcta tcgcaagatc attttacctc ttacaaaacc ggcgattgcc agtttgacga
     4141 tcatcacatt cgttaataca tggaacgatt atatggggcc attcatttat ttgtcttcta
     4201 cggaaaataa aacaatccag ttagggttga aaatgtttat tgggctattt gatgcagagt
     4261 acgcattaat catggctgcc tcagttgttt ctgttcttcc tgttgctttt atatttttag
     4321 cgatgcaaaa atattttgta gaagggatcg cttcatcagg aatgaaggaa taggaggtga
     4381 ttatgaaaga gcagcgtttt gacaaaaatg tttatatgga attcttttat tggttgtatc
     4441 aatcattggc aagtagcctt tgtttttggt gtgtcaatat cttgtatata gcagctgcgt
     4501 tccttttgtc gatagatgga aggaatgtct tttcctttat tttttcttta ctttttattg
     4561 gtccaggttg catcgtgatg ataggtatga cagaagaatt agcaaaagaa aaaagttttt
     4621 caccagtacg tttgttcata aagacgtttc gccaattttg gctaaaggga tttttttatt
     4681 ggctatttgc ttggatagtt agtgtgataa tgatttttga ttgtttcttt tttatacgtt
     4741 tttcttatgg aaaatggttg attccgttgt ttgttttact tgcttgttta tcagtttcct
     4801 ttagcatcaa ctactggtat tttcaggtga gaaatccagc gtcgaaacca aatcaagttc
     4861 tacggatagc gttctactat acgttgaaaa aatggtatgt gagtttacta gattttttac
     4921 tattgacaag tttgtttctc ttctttttcg taaagcccca atggtgtatt ctactaggtc
     4981 cttctatcgt atttggtttg atttacttca acaatcgtaa attgatgaga actatggatc
     5041 tgtagacttt ataaattgaa actttcaatt aagaatgaca gtttttatgt aaaaactgtc
     5101 attctttttc attttttgaa acatatggct aaacaaatga aataaaacgt gctatacttt
     5161 tgaatgaaac aggtttagaa actgtatcaa ttatttgtga ttatttctaa ctaataatta
     5221 aaaataaagc ataaaaagga cgatgttcaa tggacgaaaa aatgaaacaa ggttcgaaat
     5281 ctgtagtaga aagtttgatt aatcatcatg ttgattatgt attcggtata ccaggtgcaa
     5341 aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc gtgactcgtc
     5401 acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga gagccaggag
     5461 tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg gtaacagcga
     5521 cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc gatctattga
     5581 agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca aaatacagtg
     5641 cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat cgtatggcta
     5701 agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg gatgcacctg
     5761 tccaagggaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct tctgcagatg
     5821 atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta ttagtaggta
     5881 tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgga aagacagcat
     5941 tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta gaagcgcatt
     6001 tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg aaacgaagtg
     6061 atctagttat tgctattggc tatgatccaa tcgaatatga agcaagaaac tggaatgcgg
     6121 aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc
     6181 agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg acaggttcat
     6241 tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt caagcagaat
     6301 taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat cctttagaag
     6361 ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta gatgtaggta
     6421 gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat ttgttattca
     6481 gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct gcattggtgc
     6541 gtccaaatac acaaatcgta tcagtttccg gcgatggggg atttttattc tctgcccagg
     6601 atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg aatgatggac
     6661 actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact
     6721 ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga atccgagcta
     6781 caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa ggacccgtaa
     6841 ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca atcttaccag
     6901 atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag cacgggacat
     6961 taggagcttt gatggctggc ttgatggatg gaacagaaac gattgctcat atattagaaa
     7021 aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc tttttagatg
     7081 gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga agtgaactta
     7141 ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc tcaaaaactg
     7201 cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa aacctatttt
     7261 tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag
     7321 aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc caacaaagca
     7381 atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga gttgctgcag
     7441 cgggatttca cttgcatttt atcgatgata cacatacatt tggcggacat gtaatggatt
     7501 ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta cagcattttc
     7561 cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta gcagcagaaa
     7621 tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa cctgacgtat
     7681 ctcgtttttt tgaggaggaa acctagcgtt tacaaaaaaa ctgagctata atcttctcca
     7741 tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg
     7801 aaattttatt attacagcaa caaggattat tagaagcaga agataactgg ttagcaaatt
     7861 tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt gcaggatatt
     7921 atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt tcttgcacac
     7981 gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa acattgatcg
     8041 ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca caatcagaaa
     8101 tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc gattgcggag
     8161 taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta gatcttttat
     8221 tagcaaattt taatataaat gctttttcat ttattaataa ctaaaatcgt ctattgaaac
     8281 aaaaaagaag agaa
//
LOCUS       DACDEA010000093         7977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDEA010000093 DACDEA010000000
VERSION     DACDEA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.094"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(4..1848)
                     /locus_tag="IV136_002423"
     CDS             complement(4..1848)
                     /locus_tag="IV136_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466367.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2060..2578
                     /locus_tag="IV136_002424"
     CDS             2060..2578
                     /locus_tag="IV136_002424"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8466368.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2706..3389
                     /locus_tag="IV136_002425"
     CDS             2706..3389
                     /locus_tag="IV136_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8466369.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3698..3771
                     /locus_tag="IV136_002426"
     tRNA            3698..3771
                     /locus_tag="IV136_002426"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3732..3734,aa:Arg,seq:tct)
     gene            3930..4289
                     /locus_tag="IV136_002427"
     CDS             3930..4289
                     /locus_tag="IV136_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8466370.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4412..6043
                     /locus_tag="IV136_002428"
     CDS             4412..6043
                     /locus_tag="IV136_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8466371.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6105..6746)
                     /locus_tag="IV136_002429"
     CDS             complement(6105..6746)
                     /locus_tag="IV136_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8466372.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7028..7336
                     /gene="rplU"
                     /locus_tag="IV136_002430"
     CDS             7028..7336
                     /gene="rplU"
                     /locus_tag="IV136_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8466373.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7363..7707
                     /locus_tag="IV136_002431"
     CDS             7363..7707
                     /locus_tag="IV136_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8466374.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7720..>7977
                     /gene="rpmA"
                     /locus_tag="IV136_002432"
     CDS             7720..>7977
                     /gene="rpmA"
                     /locus_tag="IV136_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8466375.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         2529 a         1320 c         1638 g         2490 t
ORIGIN      
        1 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
       61 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
      121 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
      181 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
      241 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
      301 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
      361 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
      421 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
      481 taatatcttt tccacatagt ttttagcttt cgtttggtcc gtatgcatta aatcttgcag
      541 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
      601 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
      661 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
      721 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
      781 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
      841 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
      901 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
      961 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
     1021 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
     1081 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
     1141 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
     1201 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
     1261 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
     1321 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
     1381 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
     1441 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
     1501 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
     1561 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
     1621 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
     1681 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
     1741 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
     1801 taaataaaaa gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct
     1861 ttctaaaact tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata
     1921 aaaaatgaga taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat
     1981 ttttacagct ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata
     2041 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
     2101 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
     2161 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
     2221 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
     2281 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
     2341 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
     2401 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
     2461 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
     2521 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
     2581 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
     2641 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
     2701 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
     2761 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
     2821 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
     2881 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
     2941 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
     3001 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
     3061 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
     3121 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
     3181 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
     3241 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
     3301 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
     3361 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
     3421 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
     3481 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
     3541 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
     3601 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
     3661 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
     3721 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
     3781 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
     3841 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
     3901 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
     3961 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
     4021 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
     4081 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
     4141 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
     4201 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
     4261 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
     4321 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
     4381 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
     4441 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
     4501 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
     4561 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
     4621 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
     4681 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
     4741 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
     4801 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
     4861 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
     4921 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
     4981 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
     5041 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
     5101 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
     5161 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
     5221 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
     5281 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
     5341 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
     5401 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
     5461 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
     5521 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
     5581 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
     5641 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
     5701 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
     5761 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
     5821 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
     5881 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
     5941 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
     6001 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
     6061 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
     6121 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
     6181 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
     6241 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
     6301 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
     6361 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
     6421 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
     6481 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
     6541 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
     6601 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
     6661 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
     6721 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
     6781 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
     6841 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
     6901 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
     6961 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatgaga aggaggtgct
     7021 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
     7081 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
     7141 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
     7201 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
     7261 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
     7321 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
     7381 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
     7441 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
     7501 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
     7561 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
     7621 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
     7681 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
     7741 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
     7801 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
     7861 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
     7921 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaa
//
LOCUS       DACDEA010000094         7765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDEA010000094 DACDEA010000000
VERSION     DACDEA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.095"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..1927
                     /locus_tag="IV136_002433"
     CDS             161..1927
                     /locus_tag="IV136_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8466376.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2029..3288
                     /locus_tag="IV136_002434"
     CDS             2029..3288
                     /locus_tag="IV136_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8466377.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3435..4736
                     /locus_tag="IV136_002435"
     CDS             3435..4736
                     /locus_tag="IV136_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8466378.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4739..5590
                     /locus_tag="IV136_002436"
     CDS             4739..5590
                     /locus_tag="IV136_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8466379.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5603..6418
                     /locus_tag="IV136_002437"
     CDS             5603..6418
                     /locus_tag="IV136_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8466380.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6569..7735
                     /locus_tag="IV136_002438"
     CDS             6569..7735
                     /locus_tag="IV136_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8466381.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2527 a         1348 c         1557 g         2333 t
ORIGIN      
        1 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
       61 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
      121 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
      181 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
      241 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
      301 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
      361 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
      421 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
      481 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
      541 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
      601 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
      661 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
      721 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
      781 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
      841 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
      901 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
      961 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
     1021 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
     1081 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
     1141 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
     1201 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
     1261 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
     1321 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
     1381 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
     1441 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
     1501 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
     1561 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
     1621 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
     1681 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
     1741 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
     1801 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
     1861 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
     1921 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
     1981 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
     2041 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
     2101 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
     2161 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
     2221 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
     2281 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
     2341 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
     2401 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
     2461 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
     2521 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
     2581 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
     2641 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
     2701 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
     2761 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
     2821 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
     2881 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
     2941 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
     3001 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
     3061 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
     3121 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
     3181 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
     3241 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
     3301 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
     3361 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
     3421 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
     3481 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
     3541 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
     3601 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
     3661 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
     3721 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
     3781 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
     3841 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
     3901 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
     3961 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
     4021 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
     4081 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
     4141 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
     4201 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
     4261 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
     4321 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
     4381 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
     4441 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
     4501 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
     4561 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
     4621 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
     4681 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
     4741 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
     4801 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
     4861 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
     4921 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
     4981 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
     5041 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
     5101 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
     5161 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
     5221 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
     5281 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
     5341 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
     5401 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
     5461 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
     5521 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
     5581 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
     5641 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
     5701 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
     5761 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
     5821 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
     5881 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
     5941 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
     6001 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
     6061 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
     6121 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
     6181 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
     6241 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
     6301 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
     6361 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
     6421 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
     6481 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
     6541 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
     6601 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
     6661 cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
     6721 agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga
     6781 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
     6841 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
     6901 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
     6961 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
     7021 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
     7081 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
     7141 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
     7201 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
     7261 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
     7321 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
     7381 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
     7441 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
     7501 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
     7561 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
     7621 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
     7681 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
     7741 gcaaaagtac tgtttaaaga ctatt
//
LOCUS       DACDEA010000095         7743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDEA010000095 DACDEA010000000
VERSION     DACDEA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.096"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            198..1625
                     /locus_tag="IV136_002439"
     CDS             198..1625
                     /locus_tag="IV136_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8466382.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1717..2082)
                     /locus_tag="IV136_002440"
     CDS             complement(1717..2082)
                     /locus_tag="IV136_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8466383.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2079..2444)
                     /locus_tag="IV136_002441"
     CDS             complement(2079..2444)
                     /locus_tag="IV136_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8466384.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2636..2884)
                     /locus_tag="IV136_002442"
     CDS             complement(2636..2884)
                     /locus_tag="IV136_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466385.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2883..3011
                     /locus_tag="IV136_002443"
     CDS             2883..3011
                     /locus_tag="IV136_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8466386.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3068..4762
                     /locus_tag="IV136_002444"
     CDS             3068..4762
                     /locus_tag="IV136_002444"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8466387.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4829..5035
                     /locus_tag="IV136_002445"
     CDS             4829..5035
                     /locus_tag="IV136_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466388.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5413..6546
                     /locus_tag="IV136_002446"
     CDS             5413..6546
                     /locus_tag="IV136_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8466389.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6577..6897
                     /locus_tag="IV136_002447"
     CDS             6577..6897
                     /locus_tag="IV136_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466390.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6926..7390)
                     /locus_tag="IV136_002448"
     CDS             complement(6926..7390)
                     /locus_tag="IV136_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8466391.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7522..>7743)
                     /locus_tag="IV136_002449"
     CDS             complement(7522..>7743)
                     /locus_tag="IV136_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466392.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2535 a         1481 c         1381 g         2346 t
ORIGIN      
        1 tttttatttt tttatattgt taaaaattcg caatttattt tttaatagaa ttatctgttt
       61 tccaaatttt ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt
      121 aactgatgat ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac
      181 aaggcggagg ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt
      241 ttttatgtgg gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc
      301 tgcatgtttg caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag
      361 aacagatcgc ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata
      421 tacctcgatg caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc
      481 tgctttattt ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct
      541 ctgtcagcaa agcttattca ttaaaaaaac aagtattagc ttattttaat gactgcggga
      601 tcgaaatcga ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta
      661 atgtgtcgta tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac
      721 gtgccgatcg tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa
      781 ataaagaaat cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg
      841 tgacaataga ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg
      901 aatcagcatg ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta
      961 ttttgacact attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag
     1021 actttcaaca gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg
     1081 ctacctttga atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc
     1141 tgattcatct gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt
     1201 atcttttaaa tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct
     1261 ggtccagcca gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga
     1321 tcgaattgag cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa
     1381 attcggatgt tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt
     1441 ttcaagttgc accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt
     1501 cctcaaaccg ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa
     1561 atattattcc tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca
     1621 aataaggggc tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc
     1681 tgtaacaatc tcttgataca cgtcaggctc acaacctcac ttattttgga catctacggg
     1741 tccgatctct ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag
     1801 ttggaaaata tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag
     1861 aatttccaaa cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct
     1921 gactcggtcg ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga
     1981 tagacaaaca ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt
     2041 ttcgaaatat ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt
     2101 tgtttttctt tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata
     2161 ataataagtg caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata
     2221 tcagcaacga ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta
     2281 tctgcattac gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt
     2341 ttgaatagaa caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata
     2401 gcaatgatcc attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt
     2461 cttttaattt gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa
     2521 agttgttcta atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc
     2581 ctccatccat aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt
     2641 ccgtcatcag cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa
     2701 aaatagaacc atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag
     2761 gaaaatcaga cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt
     2821 ggcattctaa taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt
     2881 tcatgagtaa aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg
     2941 catatctagg aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg
     3001 aatcaatcta gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg
     3061 aatcacaatg agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc
     3121 ggctgtttat ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt
     3181 ggatgatcac ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa
     3241 taaaaaccat ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa
     3301 ttaccgtacc tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc
     3361 agggatgact gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa
     3421 agcacgtttg atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa
     3481 taaagaccgt attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact
     3541 agacaatatc actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt
     3601 ctggtttatg tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg
     3661 tcgctatatg catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa
     3721 tcgaacacgt tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga
     3781 acaaggatgc aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc
     3841 gatgcaagat gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga
     3901 agaatacgtt gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga
     3961 ttcagctact ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc
     4021 ttctagtctc tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt
     4081 ctttgctgat cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt
     4141 gttgttgaat gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt
     4201 tatttcaacc acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt
     4261 gcatcatcaa ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt
     4321 cctttatcca agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa
     4381 agaaatgcta gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat
     4441 tcgtgaaaaa gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc
     4501 ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa
     4561 acattcgacc aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt
     4621 tactgaacaa agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc
     4681 tatgtcccgt cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc
     4741 cgcaaaaaaa atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     4801 aagaacaacc aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca
     4861 gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt
     4921 aataagagca gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc
     4981 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg
     5041 agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat
     5101 accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa
     5161 aacaactatt ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac
     5221 tatttcaatg attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac
     5281 ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat
     5341 ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa
     5401 ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac
     5461 gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc
     5521 atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat
     5581 gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg
     5641 aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc
     5701 ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt
     5761 ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt
     5821 ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt
     5881 ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa
     5941 aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc
     6001 gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat
     6061 aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga
     6121 acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg
     6181 gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca
     6241 atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact
     6301 ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc
     6361 gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt
     6421 gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca
     6481 gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa
     6541 ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc
     6601 catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc
     6661 tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc
     6721 tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt
     6781 tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt
     6841 tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa
     6901 tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg
     6961 tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg
     7021 tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga
     7081 tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg
     7141 cacaaagatt tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc
     7201 ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat
     7261 tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg
     7321 aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat
     7381 atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct
     7441 ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact
     7501 tgttctccgt tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt
     7561 agctaccttg acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag
     7621 tacaccacct gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca
     7681 tggaggcata acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact
     7741 aac
//
LOCUS       DACDEA010000096         7733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDEA010000096 DACDEA010000000
VERSION     DACDEA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.097"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..2383
                     /locus_tag="IV136_002450"
     CDS             56..2383
                     /locus_tag="IV136_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8466393.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2509..2838
                     /locus_tag="IV136_002451"
     CDS             2509..2838
                     /locus_tag="IV136_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8466394.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2878..3882
                     /locus_tag="IV136_002452"
     CDS             2878..3882
                     /locus_tag="IV136_002452"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8466395.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3879..4325
                     /locus_tag="IV136_002453"
     CDS             3879..4325
                     /locus_tag="IV136_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8466396.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4475..5290
                     /locus_tag="IV136_002454"
     CDS             4475..5290
                     /locus_tag="IV136_002454"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8466397.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5496..6668
                     /locus_tag="IV136_002455"
     CDS             5496..6668
                     /locus_tag="IV136_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8466398.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6830..7324)
                     /locus_tag="IV136_002456"
     CDS             complement(6830..7324)
                     /locus_tag="IV136_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466399.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7321..7515)
                     /locus_tag="IV136_002457"
     CDS             complement(7321..7515)
                     /locus_tag="IV136_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466400.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2605 a         1449 c         1537 g         2142 t
ORIGIN      
        1 taaatttaga ataacgtgta aaggattatt tgatgatttt ttattgagag gagggatgaa
       61 aatgattaag aaaactcaaa ataaaatcca acgtatatgt caaaaacatt ttgtttctag
      121 actcaaagct gtatctaaaa gaaaatactt acatcccatt atgatttcga tcatcttgat
      181 ttttactttc ttagggagct tatcaagtat ttatgtaaac gtttatacag aaacgtcaaa
      241 ttttgctaac caaacgacag aggagacaaa tagtccaccc tattctgaaa tacagtccat
      301 agaaagcacc aatcagtcta gcaacaacga gacaacctca accacatcct cgactcaact
      361 gattgatccg acagaaaaag aaatcacagc agcaaaagca gcaggggaga aagcttattc
      421 agaaacaggt agaagccaga catttagtac tgttgcggcc actgcgaatt tcaacggaca
      481 taccatacca gatggatata cagtgatata tgccaaagat ggagtcattg ttgcggttaa
      541 cggatcgatg agtgcggata aaactgcgtt acaaggcgta accaaagcaa tcgcttttat
      601 tgataaccgt ccagatatcc ataatctgtt tattggctcc acagtaaatg gtactactat
      661 cacattccca aaaagtatct atatgggggt ggctaccgct ggaaatacga tcgattcagt
      721 tacacccaat gcggataaaa gttattcttt tacaccctca acatggggag ggcttcctgc
      781 cggaggaggt atgagtactt tttcaatcgg tatacagtta gcaaatgata ttccattcac
      841 ggggtactcg atcaaaaatg ggcagatcgt tgatggtaag ggaaatgttt caaattttcc
      901 aacctatcaa gcagcaacgg ttcaggtcaa tgcgaatcgg acaggaactt tttcttatac
      961 agtcaatggt ggagcagcaa aaatcggaac gtacgggact acattatctg gaatccatgt
     1021 gggtgacaaa gtggttatca ctccaacagc agtaacgggc tattcttttg cacaaacaaa
     1081 cggttctttc acagccctag aaagtaacgt cgatacggtg acctatacag gcaaacaaac
     1141 accaatcact attaagtatc aagactattt tggacagatg attgctcctt ctaaaataat
     1201 gaatcttact tacggaagtg ctgcacaaga cctgacgact aatacgccta ctatcaacgg
     1261 atataccttt acagctgttt cagcgataga aacaaagaac caatcagcaa tgagtgtttt
     1321 agctagttta gatacaaatg gaaatgtaat cgtaaaggac gcaaatggaa aacagatttc
     1381 tgaagtcatc ctatattata agacaaatgc aacagtatca atcaatgcaa atggcagtaa
     1441 gtactatgat ggtctttctg ttgtaccgat cgttaaatat gcctttaatg ataaaacaga
     1501 atcaacatcc cttttaaaca atgatctcag tattgatcaa atcacatgga atccaactga
     1561 tttcaaagcg atgaatgaaa agggagttga agtaactgcg cctactgaaa ttggaaccta
     1621 tacgtcttgg caattgacaa aaagcggact agctaaacta gctgccagaa caaactattt
     1681 atttactatc gtgcagacat ctgatgtcta tacgattaag caaatcagcg gtgcggtaga
     1741 gctcagtggc agtaagacat atgatggaaa agcaggtgtc ccgtcaatcc atgtcaagct
     1801 agcagagggt gtcactagtt cgcttactcc agtacaagtg gcacttagca gtatggatta
     1861 cacagcagat gcacaatcag caaaaaatat ggttaatgct ggcagctata caattaaact
     1921 aacgaatagc ggaatagaca aagtcaaagc agcaaatccc aagttgtcat ttactgatat
     1981 agccaacaca tcaggaactt ataccatcta taaagctaac gcagtgatta ctgtcgatga
     2041 cgctgccttt aattatgatg cccaaagtca ctcgatttcg gttggaaatc tccacgttac
     2101 cggtgttgtc ttaggcgaat cattggatta taccctaaca gttaacagcc ggacagatat
     2161 tggatcacaa atagtaacga ttagtttagg cgaaggaagt gtaaacaata attatacgat
     2221 tatcgcaaca gataccgcta agttgacgat caatccgact ctcttgcttc cttcgacagg
     2281 tgggataggt ctactgccct ttgtcttttt aggactgatc tttatttttt ctggcttctt
     2341 ttattttatc catcgtaaga aaaaagcagg tgaacagcga tgaaaaaaac aaaaaatgta
     2401 cgtgtggata tatttgctgt tctctttttt atcctactgg tcttttttat aggttctccg
     2461 actgtgtcag ctagagaatc acctattaca tttactttgc ataatcgaat gttcgaaagc
     2521 gataagatgc caagtatcag tcaaaacgac ggaataaata tcccggataa ctttcaagga
     2581 gtaaaagggt tgaacggcgt tactttcgct atatatgatg tctcggatga attttacaag
     2641 ctgcggtcag aaggaagtag tgtggaagac gcacaaagaa aactagcaca aaaaagtgat
     2701 agtgaaaaaa tactggcaga aaatgtgaca aaaacagtag acgaggaaga gggcattgct
     2761 tcttttagtg catcggacaa agatgagcaa ggtcgtgacg ctgtttaccg atttacggaa
     2821 ataaagactt cagattaagt aaaagaaaaa tcagcaccat ttgtcgttgt gcttcctgtg
     2881 acagattcat ctaatcataa attgacgacc atccacttgt atccaaagag cgaacaaaaa
     2941 atacctgctg cactcacatt aacaaaaaca gtagagaata agcagacaga ttttgcagat
     3001 ggggataaag tcccttatct catcaccgta acaattcctg agaacatcaa cgagatagga
     3061 acctatacga tcaaagatac agctgatcct cagttatggc tagaaccaga aacgatcgat
     3121 tttttgattg gtggagacaa gattcataca tttcacacac aaaaaataaa acatggtttt
     3181 gtcttatcag attctggaaa agatcttagt tcgtttgctg gaaaaaaatt aacgatccgt
     3241 taccaaatga cattgaaaga aaacagtgaa aaaacgacat ttgataacga tgtacgtttg
     3301 acgactgaca atcaaacagt agaaacacat cttgccatcc aaacaggagg caaacaattt
     3361 gtcaaagtgg atcggcaaac gaaacaaaaa ctagcagatg ctcaatttct agtcaaaaaa
     3421 aatgaccaat acttagcgca agaaaacggg ataaatcatt gggtagccaa agatgattca
     3481 agagcaacga tttttacttc cagtaaagat ggaacatttt ctgtccatgg attgtctttt
     3541 ggaagttacg aactagtgga gattaaaccg cctaaaggat atatattgag tcaatcaacc
     3601 attctctttg aagttgaaaa aggcagttat gatccgcttc atcctatacc attggaaatc
     3661 atcaacgaac caaaagcaaa tccgacgaat aatggaaaaa cgccagttca gatgcagaca
     3721 aatggtccat caaatgaaaa aaatggagga agctttccaa aaacaagcga agagatgctt
     3781 ggaggtttct caattttagg gttattgcta gtcctttcaa cagggacagc ttggttttat
     3841 aaaaaacaac aaaaaggaaa caataggagg gaaataaaat gaaaaacatc aaaacaaaaa
     3901 tatggacgtt tttaggaact gctatcatgc tgcttccttt cgttctcggc ctaggaactg
     3961 cagaggtttc tgcagccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat
     4021 tgaaattcac ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga
     4081 atagcgaacc gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc
     4141 caagtaaaga cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg
     4201 gtgaaggatt agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg
     4261 tctttgtaga aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac
     4321 cctagaccct gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga
     4381 aagcaatatc aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc
     4441 gcaattccag atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg
     4501 tgtcgctctc tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc
     4561 catcagtgtc aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga
     4621 tggttatcaa ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt
     4681 gcaacaaaca accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg
     4741 tgtagtcaat cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa
     4801 ctacggtgat ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc
     4861 agaattttct attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa
     4921 ccttgcctca ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga
     4981 agaagtgaaa ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg
     5041 ggcatactgc cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc
     5101 tgcgtacact gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga
     5161 taaccaacct aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt
     5221 ggtaggtact gctttagttg gaggagcagt gatctatttc aaaaaacgcc atcaagaaac
     5281 agaagcttaa actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg
     5341 gggagaaagg ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata
     5401 ggaggaacta ttttgccgtt tgcttcgcta atactcaggt caaggcgctg atttcacaac
     5461 ctcttcatat acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac
     5521 tggacatctt catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg
     5581 tagaagattc actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag
     5641 catcgaataa gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc
     5701 aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg
     5761 caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca
     5821 tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt
     5881 tgcaaaaagg aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc
     5941 atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta
     6001 agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag
     6061 tagaaaaaat ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca
     6121 gagatctcgt gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg
     6181 tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg
     6241 atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg
     6301 gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc
     6361 ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg
     6421 taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt
     6481 ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac
     6541 atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga
     6601 aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga
     6661 aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa
     6721 gctgacgaaa tggacgtgta aactgttatt tttggtttac tatctggaac gccatcttct
     6781 atttttttat gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat
     6841 aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat
     6901 acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag
     6961 tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg
     7021 ctgattgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt
     7081 ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt
     7141 cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag
     7201 caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag
     7261 ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg
     7321 tcactttttt tctagtgcag ctttttccgc tttttgtttc tcttcttttt tcttttcaat
     7381 ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7441 agcctgatct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7501 acttcctgcg accaaaaaca atgcaactaa ttttttcgct aaatttcctc cttaagtaaa
     7561 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7621 gttatgaata gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg
     7681 ctatagttct gttattaagt atgcaataat aaaatatgta cgttatttta tta
//
LOCUS       DACDEA010000097         7651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDEA010000097 DACDEA010000000
VERSION     DACDEA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.098"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..498
                     /locus_tag="IV136_002458"
     CDS             19..498
                     /locus_tag="IV136_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8466401.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            591..1753
                     /locus_tag="IV136_002459"
     CDS             join(591..867,867..1753)
                     /locus_tag="IV136_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8466402.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2033..3013
                     /locus_tag="IV136_002460"
     CDS             2033..3013
                     /locus_tag="IV136_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8466403.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3135..4553
                     /locus_tag="IV136_002461"
     CDS             3135..4553
                     /locus_tag="IV136_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8466404.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4624..6093
                     /locus_tag="IV136_002462"
     CDS             4624..6093
                     /locus_tag="IV136_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466405.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6103..6570
                     /locus_tag="IV136_002463"
     CDS             6103..6570
                     /locus_tag="IV136_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8466406.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6606..7478
                     /locus_tag="IV136_002464"
     CDS             6606..7478
                     /locus_tag="IV136_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8466407.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2630 a         1157 c         1573 g         2291 t
ORIGIN      
        1 atagaaagag gtgtttatgt ggttgataaa attcagttta atcaagttat agaagaattc
       61 atcaaagtaa ataatttaat gacccatatt caaaaacgac caatcagaat aactaatgat
      121 ctaaaactca gtacgagtat gattcatttg attgatgtga ttggaaatta tcctgaaact
      181 agtattacgg aattagctga tcgattaggc gttaccaaag gagctgtatc ccaacagatt
      241 cccactttag agaaaatgaa tcttataagc atttctcaaa aaaaaggaaa taagaaaact
      301 aaacttgtat cgttaacaaa atatggtcga aaggtgtacg aatcacataa ttatttacat
      361 gaagagcttt atagctcaat aaatgaagcg ttagaatcat tctcaccaga ccaaatttca
      421 accattaaac agattctaga aaaagtatct tatacaatca atgaatatca agtaagtctg
      481 acaagaaagg aagattgaca tgaaaaaaat gatttttgaa tcggctacac tagactagac
      541 agaaaaaata aggtgtgtaa aataaatgaa aacacactgg aggaattttt atgtcaagac
      601 gtcaacgtag aacctattca aaagaattca aacaacaagt cgtcgatctt tatctcgcag
      661 gaaagccccg agctgaaatc attcgagatt atgagctgac tacctcggcc tttgataagt
      721 gggtcaagca agcgcaaact actggctcgt ttcaggaaaa agataacttg acccctgaac
      781 aagtagaact aattgcatta cgaaagaaaa ataaacagct tgaaatggag aatgacattt
      841 taaagcaagc ggcgctgata ttgggacgaa aagacaagta attgatacca ataagcacaa
      901 atactcaata tcagcgatgt gtaaagtgtt aaacatctct tgccaaacct attattatca
      961 agccaaacca gttgaagatg aatcagaact ggaagaactc gttcaagaag aatttattcg
     1021 caatcgaaaa gcttatggta ccaggaagct gaagcagtgc ttggccaatc taggtcttca
     1081 agtgagtcaa cgcagaatcg gccgtatgat gaaacagcgt ggcttgcgtt caacatacac
     1141 attggctcat ttcaaattcc attcttctac ttgtaacgaa gccaagaccg ccaatattct
     1201 agacggaacc ttcctacagg aaaagtcatt agaagcgatt gtaaccgacc ttacctatgt
     1261 tcgtgttggg aagaaatggc agtatatctg cctgattctt gatttgttta atcgagaaat
     1321 catcggctat tcatgcgggg agaagaaaga tgcgacgtta gtcaaggaag cttttgcaaa
     1381 gattccgtac tcgttgaata atgtacaact ttttcacacg gatcgtggaa aagaattcga
     1441 taaccagacg attgacgaaa ttttatgggc ctttaatatc acgcgttcct taagcaaaaa
     1501 aggctgcccc tatgataacg ccgttgcaga atccacctat aaatctgtca aagtagaatt
     1561 cgttcatcaa taccgctttg agacattggc taatttgcga ttagagctat ttgattacgt
     1621 ccattggtgg aataccatcc ggctgcacgg cacgttaggc tatgagacac cgatcagttt
     1681 tcgacagcag agattggcgc agcgaatcct tgataatgag ctcggatgtg ataccggtgg
     1741 cgaggcacct taattatttg aatgagcttc tgccttagag aatcagattc gaggaggctc
     1801 gttgtcaagg gcagtcggaa caccagcagc aagcttacgc aataccttat aatttttgtc
     1861 aaaaaaagtg ttgccattcc acatatatct ttaaacagct attgccagat aaaaataaat
     1921 gatattatat atataaatac ttatccgaaa aggggaatga gagagtcaag gttcttggaa
     1981 tgtcgaatag tcgagtaggt agattttcta tctacaacct tgttgtttaa aaatgaaaaa
     2041 aattggaatt agagaagttg cgaaagaggc aaatgtttct cctacgactg tgtcgagggt
     2101 attgaatgat cgtggatatt tgagtgagga aacaaaaaga aaagtttttg atgccatgaa
     2161 aaaattagaa tactatccta atgaactggc gagagcactt tttaaaaacg aaacatatac
     2221 cgtaggtttg atttttccta caattactaa tccatttcac gctgagttaa ttcaagatat
     2281 cgagttcgag ctttccagca ggggatataa agtgttgctg tgcaatagct taaacaatcc
     2341 aacaaaagaa aaagagtact tgacgatgtt gagaaagaat caggttgatg gaattatagt
     2401 aggaacgcac aataaagata ttgcggatta tgatattcca gggctaccta ttgtagcgat
     2461 tgatagaagt cttggtgaaa atacagttac agtatcttgt gataattaca ggggtgggga
     2521 attagctact caactacttg taaatcatgg ctgtcagaat atactgtgta ttcggggaga
     2581 tagtaagata aagctacccg cgaatagtag gactttagca tataaagatg ttttgaataa
     2641 attcatgctg gaaccgttag tcctagaagt accatttgta aaagaaatca atgaaaaaaa
     2701 tgaaataatt aagacatatc tggaaactca tccaaatatt gacggggttt ttgcaggaga
     2761 tgatttattg gcttctttgg cgattaacaa cttaggaagt tcgggcaaaa gagttccaaa
     2821 agaggttaaa gtgattggat ttgatggagc tcaacaaaca ttgatttata atccagaatt
     2881 atcaactatt caacagccta tagatcaaat atctaaagta gcagtaaaaa aattattgaa
     2941 tatgattaag ggcgaaaagg agacggatga actttcactt ccagtaaagc tcataaaaaa
     3001 aaatacagca taagttaaat cggcgaaaaa ttccttcgtc gatttaaaaa tatttttatg
     3061 aaacgggttg acatatgaaa cgggttgaca tatattagtt gtgaagataa aaaccttttc
     3121 actggaggaa gaaaatggag aaattattaa ttaaaagcaa taatcatcaa ctttactacc
     3181 aacctaagga tgtttgggtc ggagatgtta tgccttacgg gaaagaagga aaattcttta
     3241 tttatcatca acgtgatact agaaatccag ttccttttgg agaaccgttt ggctgggctt
     3301 tagcaactac gaaagatttt gttcattttg aagactttgg ggaatctctt ttaagagggg
     3361 atgacaatga gagagaccag tttatctacg ctggttctgt atttgaggca aatggaaagg
     3421 ttcacgcatt ttatacaggg tataatcgtg aattcttagc gcagggaaag acatctcaag
     3481 tgttgcttca tgctgagagt gatgattttg ttcattggga aaaatcagaa acagcattgg
     3541 agttagaacc acaagaaggg tatgatattc gaaactggag agatccattt gtcgtttggg
     3601 atgatgaaaa agaagaatat ttgctaattt taggggcgcg aaagggtcag gataaacatc
     3661 tacaaaccgg acgtttagtg gaatttacat caaaagattt aactaattgg gaattcaaag
     3721 gtgatttttg ggctccggga ttgtatacca tgtttgaaat gccagaactt ttcaagattg
     3781 gtgactggtg gtatcttgtc tattcagaat actcagatgg aaataaaata ctctatcgaa
     3841 tgagtcaaag tttggaaggt ccttggatta agcctaaaga tgaagctttt gatggacggg
     3901 cttattatgc aggtagaaca gcttatgatg gaaaacgtcg agtcctattt ggatgggtac
     3961 caacaaaaga tcaagaaaat gataaaaaca attatttatg gggaggtact ttggttcctc
     4021 atgaagtcta tcaaaaagag gatttttcac ttggggtaaa acccatagac gaagtgtgga
     4081 atgcattcat aaatttacat gatattgccg atgtagagat agaaacagca gacacaagag
     4141 aagaagtaac gcttgtcaaa aatagtggaa ctttgtttaa tttagaaaca acaatttcat
     4201 ttgatgagaa tcttagtgac ttttcactga gattatataa aaaccttgag acagatgaat
     4261 cttacgaata tcgcttcaat ttagatgagg ggcgtcttac atttgataaa aacccttgtt
     4321 acccttggtt tcaatatatg gataaaggac ttattagacc tatatcttta aagcctaacc
     4381 aatcttataa actaaaagtg attgttgatg acaatataat gacactttat attgatggaa
     4441 tcgctcttaa cgctcgtgta catggttgtt ttggtcaagc aattactatt acagcaacaa
     4501 atggatcaat gactgcaata aataccaagt tttcaaatga aataaagaaa taataaacgt
     4561 caatcatgtg gcaggtctga aaatcaaaca tgacctgtca catttaaaag ggaggataaa
     4621 tgaatggact acaaaaaaat tgccgaagat gtattggaaa acgttggagg aaaagagaat
     4681 atctcaagcg ctacacactg cattactcgt ttacgtttaa ttcttaaaga caaaagcaaa
     4741 gcgaatatgg aaatgcttga ggaaatagaa ggtgcaaaag gtgttctttt caatagtgga
     4801 cagttacaag taattttcgg aacaggtata attgaaaagg tttacaaaga gttcgttgaa
     4861 ttgacaggag ctaaggaagt atccgtttct gaaatgaaag acgaaggagt caataattca
     4921 ggaaatagac ttcaacgagc ttttaaagtt ttctcggata tctttattcc gatcattcca
     4981 gcttttattg gggcagcaat ggttttagga attcgttcgt tgattaccac tcctggaatg
     5041 tttggaatgg atggctcatt cgctgatcac aatgtattcc taaatgattt atccaaattt
     5101 ctgggtatta ttgcaaccac atttgattat ttaccagttt tagttatgta ttctgcaaca
     5161 aaacgttttg gcggaaaccc tattttagga ttacttgtag gatttgttat gattcatcca
     5221 ggactagcca atcggaatga tttcgtcatg ggaactataa agcctgatta ttggcattta
     5281 tttggattaa gcattccagc agttgcgttt caagggggtg tttttccagc aattttgact
     5341 tcttggtttt tagcaaaagt tgagaaaatt acaaataaat attctcctca agtacttgct
     5401 tacattttag tcccaactgt gacaattgtt cttgccaact ttgcactatt tttagtgttt
     5461 ggtccattgg ggaattttat cggtaatgtt ttaggtggaa tcattgattt cctatatatg
     5521 aagatgggtg cctttggtgc ttttgttttt gcaggcgcgt tacaaccatt agttgtaaca
     5581 ggaacacaac atgctattca aggtattgag gcaaacttag tcgcaacaac tggattcaat
     5641 tatatccaac ctatttggtc agtttcaatt attgcccaag gtggcggttg tattggtatg
     5701 tatttactct ttaagaaaaa atctaaagat agagatattg cgatttcaag ctttattcca
     5761 acactggtag gtattactga accggctatc tttgctgtta atttgaaata ttcaatcgtt
     5821 ccttttgttt gctcgtttat tggtgctggt tttggtggag catttatgaa aatttttgat
     5881 gtaaaagcca ttggacaagg attgactgta ttgccaggtc tgactattgc taatccagtt
     5941 cttccttata tatttggtaa tctcatagca ttcattttac caatcatttt tattctcgct
     6001 tatgataaat ataaagtgtt tatccctgaa gctaaacacc gtaaatttgc agaaaaaact
     6061 gactcaaaaa aattgccagt tcaagtaaag tgagatgaga gcatgttaaa tttttttaga
     6121 aaaaatgaaa taagcgttgt agcacctgca accggtgttt ttgaaaaact ggagaaatta
     6181 agtgatccag tcttttcaaa aggaatgatg ggacaaggat ttgcaattga tgcaaaaaac
     6241 gaaacgattg tatctccaat cgatggtttt gtttcaagta tttttcctac aaaacatgcc
     6301 ttaggaataa aaacaaaatc aggaattgag atacttgttc atgtaggaat agatacagtt
     6361 gagctaaatg gtgagggatt tgatattaaa gttcaagaag gacaaaaagt taaggctggg
     6421 gatattctta tgaccattaa ttttgtagtt tttgaaaaga atcacaagac taaagatgtc
     6481 attgttgttt ttccagacta ccaagacgaa attttcattc cgaaacttga agaagtaaaa
     6541 caaggacaag aattgtttgt tcttggctaa tgataagtaa ttaataatag aatcgaggaa
     6601 aatgtatggt ttttggggct attgaagctg gtggaacaaa atttgtttgt gccattggag
     6661 ataaagattt gaacatattt gaaaaagtca gctttcccac gactacaccc aaagaaacaa
     6721 tgaatttagt tttagatttt tttaatcatc ataaagatga tttagaaggt atcggagtgg
     6781 gatcgttcgg tcctattgat attcattgtg aatctaaaac gtatggcttc attacatcaa
     6841 cgccaaaact tgcttggcag gattttgatt ttattggaag tttaaaaaag caacttaata
     6901 ttccaactgc ttggacgact gatgtaaatg cctcttgcta tggtgaatat gtttctggaa
     6961 atggcagagg cctttcaagt gttgtttact ataccgtagg aacgggcata ggaggtggcg
     7021 ctatccaaaa cggaaacttc attgaaggtt ttagtcatcc agaaatggga catatgcttg
     7081 taaaacgaca caataaagac aacttttctg ggacatgtcc attccacgga gactgtttag
     7141 agggcttagc tgctggtccg gcaatcgaaa gaagatcagg gaaaaaggga caagaagtga
     7201 ctgaaaatga cgttacttgg gaaatagaag cttattacat tgctcagtgt gcatataata
     7261 caacattgat gttatcacct gatgtcatta tatttggtgg cggagtaatg aaacaaaaac
     7321 acttaaaaaa gaaagtacaa gataagttta ttgagttgat caacggatat gtcgctattc
     7381 ccgatgttga caattatatt atgacaccaa aattgttaga taattctggt attgtaggtt
     7441 gtttagcttt agcaagagac gttaaagaaa aatcttaatt tggaaaaaag atcgtctcga
     7501 ctatttgttt ggttgatgcg atcttttttt tcaattagta attgaaggga cgcctaaaag
     7561 tgaaaaaaaa aatgaaagat gaagtattag agtatatcaa aaataattta aagtattacg
     7621 atttttctgc tcaagatatt gcaatgaaat t
//
LOCUS       DACDEA010000098         7436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDEA010000098 DACDEA010000000
VERSION     DACDEA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.099"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..608
                     /locus_tag="IV136_002465"
     CDS             135..608
                     /locus_tag="IV136_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8466408.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            605..2737
                     /gene="feoB"
                     /locus_tag="IV136_002466"
     CDS             605..2737
                     /gene="feoB"
                     /locus_tag="IV136_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8466409.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2739..2873
                     /locus_tag="IV136_002467"
     CDS             2739..2873
                     /locus_tag="IV136_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8466410.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2967..3611
                     /locus_tag="IV136_002468"
     CDS             2967..3611
                     /locus_tag="IV136_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466411.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3798..4991
                     /locus_tag="IV136_002469"
     CDS             3798..4991
                     /locus_tag="IV136_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8466412.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4984..6711
                     /locus_tag="IV136_002470"
     CDS             4984..6711
                     /locus_tag="IV136_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8466413.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7105..7302
                     /locus_tag="IV136_002471"
     CDS             7105..7302
                     /locus_tag="IV136_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8466414.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7304..>7436
                     /locus_tag="IV136_002472"
     CDS             7304..>7436
                     /locus_tag="IV136_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8466415.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     "
BASE COUNT         2429 a         1270 c         1644 g         2093 t
ORIGIN      
        1 ataattgaca ttctcaatta ccgagagtat tctatgtgag aatgattctc atttatttgc
       61 gtaacacgta agaaaattag aacatataat gaatgatttt aatacatgat ttatttcaca
      121 agagaggaat caatatgcag accttagcgc aggcaaagca gaatttcgtt tatcaagtcg
      181 aacgaatcga aacagaagaa aaaacaaaac gccatttgca aaatatcggg atcgttgccg
      241 gaagtaaaat cgtcgtcgtc aatttatcag gcaacaatgc cattttactg gtaaaaagtc
      301 atcgagtggc agtaagtcgg gaaatactag gtcaaatctt tgtcaataaa aagactgctt
      361 cctctgaaag ctggacttct cttgatcaat taaaagcagg agagactggg atcgtcgtag
      421 caatccacgg acaaggcgca gtgaagcgac ggttaatgga tatgggattg acgcgaaatg
      481 taaaaatagt tatccgaaaa ctagcccctc tgggagatcc aatcgaactg acagtgcgag
      541 ggtatgaact gacgttacga aagtcagagg ccgaactaat cttgatacaa agggaggaaa
      601 aagaatgaat atcgcgttag tcggaaatcc aaacagcggg aaaactagct tgttcaatct
      661 cttgactggt tcgaatcaat atgtagggaa ttggccgggc gtaacagtag aaaaaaaaga
      721 aggatcgttg aaaaaaaatc cgaacattca tattcaggac ctacccggca tctattcttt
      781 gtctccttat acaccagaag aagtgattgc aagagattat ttggttcagg aacagcctga
      841 actgatcatc aacattattg atgctaccaa tttagaacga aatctttatt taactacgca
      901 gttattggat ttagacattc ctatgatcat tggtctgaat atgatggatg tcatcaaaaa
      961 agaaggaaaa aaaatcaatc tagaaaagct ttcctatggt cttggtgtac cagttttacc
     1021 aatcagcgta ttgaaaaagc aaggaatcga aaaactaatc gaaaagattc aggagtctgc
     1081 aaaaaagcgg ccttctattc ctgttccctg tacatatgat ccacgtttag aagcagcttt
     1141 acatgaaatt gaagatgtac taggagaaca aagcggcaag aaacgctggt atgcaatgaa
     1201 atattttgaa cgagatcaga aggtaagaga ggattacgag atagcagttt ctcagcaaaa
     1261 agagatcgag caactgatcc agttaacaga gaaactattg gacgacgata gtgagacgat
     1321 tctggtcaat gaacgttatg aatttattac ccaactatgt acactctgtg ttgtgtcaaa
     1381 tgattctttc caattatcga tgagtgacaa aatcgatcag attgcaacga atcgttggtt
     1441 agccttgccg atcttcgcat ttgtgatgtg gctgatctat tatctggcta ttcaaacagt
     1501 aggaacaatg gggacagatt ggatcaatga cacattgttt ggtacatggt taccagaaca
     1561 tgttggcaga ttgttagctg aatggcaagt agcgggatgg atgcaggagc tgatcttgaa
     1621 cggcatcatc gcaggcgtgg gagcagtgtt aggatttcta ccgcaattga ttatattgtt
     1681 tttatgcttg agctttctgg aagactgcgg atatatggcg aggatcgctt ttgtcatgga
     1741 tcgacttttc aggaaattcg gtctttcagg aaaatccttc attcctatgc tgattgctac
     1801 aggatgtgga gtgccaggcg tcatggccag tcgcacgatt gaaaacgagc aagatcgtcg
     1861 tttgacgatc atggtcacaa ccttcatgcc ttgttctgca aagttgccga ttattgcctt
     1921 agtagcaggg gccttttttc ctcatcaatc ttgggtagct ccttctgcct attttcttgg
     1981 tatgactgca attattttct ctggaatcag cttgaaaaaa acacgtttgt ttgctggaga
     2041 tacagctcct tttatcatgg aattgcccgc atatcatttt cctcaatggt cagcggtcct
     2101 aagacaaacc tatgaccgca gcaagtcttt tgttaaaaaa gcaggaacga ttatctttgt
     2161 ttctagtatc gtgatctggt tcagctcttc ttataatttt tatgctcaac ctgtttctga
     2221 agaccaaagt atcttagcct ttttcgggcg aatcctggca gttgtttttc agccgcttgg
     2281 ttggggaaac tggcaaggaa cagtcgcagc gattacagga ttggtcgcta aagaaaatat
     2341 tattggcaca tttggtattt tgtttggtca tgcggaagta tccgaaaatg gcagagaaat
     2401 ctggcaagtg ctacaacaca catatactcc cgcagcagct tattcattgt tagccttcaa
     2461 tttactttgt gcgccttgtt ttgcggcaat tggggctatc catcgggaaa tgaatgccaa
     2521 gaaatggact tggatcgcca taggctatca atgcggttta gcttatttag ttagttttgt
     2581 gatttatcag ttgggacact tattggaggg tggacaggta gcgttaggga cgtatcttgc
     2641 tattttactg atgataggac tcgtttatgt tttgttccgt cagccaaaat ccaagaacca
     2701 gttttatact atattgtcat tagaaggaga ggaataggat ggcgacattc ttattaagtg
     2761 tgttaatctt tggaagtgca ggcgcaattg tctatactcg tttaaaatcc ggtaagaatt
     2821 gtgatgactg tcaaacagct tgcccagtaa aaaaggaaca gatcaagcga tgatcagtcg
     2881 ttgcaagagg ctattctccc cttttttgct tattttgcta gagcatagta agatgaggga
     2941 aagtaaaaaa agaaagagga aagaaaatgt ctggaaagaa aaaaatcgtc cgcccgcgct
     3001 accagcagat tgctgttgat ttagccgaac gtatcgttga aaaccgatat aaagtgggtg
     3061 agaagatcca tgcccgctca acacttgcca gtacgtacaa tgcttctcct gagactaccc
     3121 gcaaggctat caatgtgctg gtagatcttg gaatcatgga agtacggcat ggaagtgggg
     3181 catttgttgc ttcgaaggaa aaagcaaaag atttcctatt gcaatatcaa gatgtacaat
     3241 ccatccaaga tacaaaagca gaaatcatgg caagtgtgga gaagcaacaa gatgagctga
     3301 atcatttttc tcaattattg gatcagctgg tcaatcaaac gaagcgaata cataagatga
     3361 atcctttact tccgtttgaa ttatctttat cagaaaaagc aatgcatctg gaccgttcaa
     3421 ttagcgagtt gaatatctgg caggcgactg gcgccacagt tatcgcaatt tctcataatg
     3481 aagaactgct tgtttcacct ggcccctatg ctaaatttac ggcaggggac atcgttcatt
     3541 ttattggaaa tgaatataca ctacagcgta tgacaaactt tttttacccg gagcatcagc
     3601 ttgcagaatg attgtgtgaa atattaaaaa aatattaact tggctaaacc ttttatttac
     3661 aaagatttag ccaagttttt ttcagtttgg acaagcaaat ttctttcatt tttgacaaag
     3721 tgactacatg atgttaacat agtgtggtca cttttttcag aaagaaaaat gaatttacat
     3781 aaaaaaggag aatttgtttg gtaaaagtag aagtaaaaca tttaacaaag atatttggta
     3841 aaaagacaca agccgcttta gatatgatga atgatcacca gccgaaaacg gagattttaa
     3901 agaaaaccgg tgcaacggtt ggtgtgtatg acgtgaattt tgacgtaaaa gaaggagaaa
     3961 tcttcgtgat catggggctt tccgggagtg ggaaatccac attgatccgt ctattgaatc
     4021 gtctgattga gccaacttcg ggcagtatct atattgatgg agaagatgta tcgaagttaa
     4081 gcaaagaaga attgcgagaa gtccgtcgtc ataaaatcaa tatggttttc caaaacttcg
     4141 gtctgtttcc acatcgaacg atcttggaaa atacagaata cgggttagaa gtccgtggtg
     4201 tacctaaaga agaacgacaa gaaaaagcag aaaaagcatt agaaaattct agtttgcttt
     4261 catttaaaga ccaatatcct agtcagttat ctggtgggat gcaacaacgg gttggcctag
     4321 ccagagcttt agcaaatgat cctgagatct tgctgatgga cgaagcgttc tcggctcttg
     4381 atccgctgat ccggcgagaa atgcaagacg aattactgga cctgcaagcc aatgttcaaa
     4441 aaacgattat tttcattacc catgatttga atgaagcttt acggattggg gacagaatcg
     4501 ccttgatgaa agacggcgaa atcatgcaga tcggaacagg tgaagaaata ttgaccaatc
     4561 cagcaaatga ttatgtacgt gaatttgtgg aagaagtcga tcgttccaaa gtgcttacgg
     4621 ctcaaaacat catggtacct gcattgacga cgaatatcga atctgatgga ccaaacgttg
     4681 cattgacaag gatgcgtaat gaagaagtca gcatgctgat ggctgttgac agaaagagac
     4741 acctgaaagg gatcatcact gcagatcaag cgttagaagc taggaagcaa aaacgtccat
     4801 tgatcgactt cctagatgaa aatgtgacgg tgattgggaa agacatggtg gtaagtgata
     4861 ttttcaatat tatttatgac tcaccgactc cacttgcagt tgtagaggat ggaaaattaa
     4921 aaggtgtcgt catccgaggt agtgtgattg aagcattagc agaaacaagc gaggtgagtg
     4981 aacatgaata atctattgaa ctatcaatta ccagtggcaa actgggtaga aaatatcaca
     5041 gaatggttta cgacaacttt ttctggccta ttttcatttt tacaaacgat tggacaggca
     5101 gtgatgtcgg ggattacaaa tttactgtta gtgattccag ctcctttgtt tatcttgtta
     5161 ttgacggtag cagctttctt tatttctaag aaacgtccag gacttacgtt gttcacatta
     5221 atcggtctat ggtttattta taaccaaggt ctatggaatg atttgatgaa tactgtcaca
     5281 cttgttcttt tatctagtgt gatctcgatc atcattgggg taccattagg gatcttgatg
     5341 gcaaaaagca gtaaagccca aagtattatc aaaccgatcc ttgactttat gcagacaatg
     5401 ccaggattcg tttatttgat tccagcagtt gcatttttcg gtatcgggat ggttccaggg
     5461 gtatttgcct cagtcatctt tgctctgcca ccaaccgttc gttttacgaa tttagggatt
     5521 cgacaagttc ctaaagaatt agtagaagct tctgattcat tcgggagcac aagtcgtcaa
     5581 aaactattca aactagaatt gcctttagct aaaagtacga tcatggcagg gatcaatcaa
     5641 acaacaatgc tgtcactttc aatggtcgtt attgcctcta tgatcggtgc acccggctta
     5701 ggacgcggcg tattatcagc attgcaaaga gcacaagtcg ggaatggatt tgtcaacggg
     5761 gttgcattag ttatcttagc aatcatcatt gaccgtttta cacagcattt gaaccagcca
     5821 aacaataaaa aagctgcagg agcaacaaca cagaaaagta aaaaacgtca aggactgatc
     5881 attggtgctg tagtagtagt gatcttaggt gcgattggta tagggagttt ttcatctgcc
     5941 aaagaaacaa aacgtatcaa cttatcttat gttgaatggg atacagaagt tgcttcaaca
     6001 aatgtagtcg gtgaagtatt gaaacaaatg ggctatgatg taacgatgac gccattagat
     6061 aattcaatca tgtggaaatc tgtttcaaat ggcgagtcag atgccatggt ttcagcttgg
     6121 ctacctaaaa ctcatggttc acaatatgca caatataaag atcaagtcga agacctaggt
     6181 gccaatttga caggagcaaa agttggttta gcagtgccag cctacatgga tgtaaattcg
     6241 atcgatgagt taaccgatca ggcaggtaag aaaatcatag gaatcgagcc aggagctggt
     6301 gtagttacgg ctgctgaaaa cacgatccaa aaatatgaca acttgaaaga ctggaaagta
     6361 gagacatctt cttcaggagc aatgaccgtt gcactaggtc aagcgatcaa aaaacacgaa
     6421 ccaattgtag ttacaggatg gacacctcat tggatgtttg ccaaatatga tctgaaatac
     6481 ttagaagatc ctgaaaacgg aatgggaagc gaagagcaga tccatacgat ggttcgaaaa
     6541 ggattgaaag aagaccagcc agaagcttac aaagtattag acaatttcca ttggtcagaa
     6601 aaagatatgg aaaaagttat gttggaaatc aacaatggaa aagatccaca gcaagcagca
     6661 aaagactgga tcaaagagaa ccaagaatta gtcgaaagct ggaaaaaata aaaagacaga
     6721 cacgaaataa atggagcttc caaaattgga tcaaatatcc aaatttggaa gctccatgtt
     6781 cgtctctatc ttttttgtct ataaaaaaag agaatgcgta gctcatcttc gaaacgtcca
     6841 aagacgccga caaactatta tgaatttatg ggtgcgagtc aaagagatcg atcgtaggta
     6901 tgaaagacta atgtgattaa agtttattat gtacgaagtg cattctcaaa aaatattaaa
     6961 gaatatacat agtttatata cttaaaagtg attaaattct tattcaggaa ttattttgct
     7021 acgtttcaag aaggaattgt tttttgaaat ttaatgaatg ttagaataat agtctaaaaa
     7081 cattaattta ggggtgatta gattatgaaa catttaaaaa ttttgtctat taaagagaca
     7141 caacttatct atgggggtac cactcatagt ggaaaatatt atggaaatgg agtgtattgc
     7201 actaaaaata aatgtacggt cgattgggcc aaggcaacta cttgtattgc aggaatgtct
     7261 ataggtggtt ttttaggtgg agcaattcca gggaagtgct aaaatgaaaa aaaatgctaa
     7321 gcaaattgtt catgaattat ataatgatat atctataagt aaagatccta aatattctga
     7381 tattcttgag gttttacaaa aggtatattt aaaattagaa aaacaaaaat atgaat
//
LOCUS       DACDEA010000099         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDEA010000099 DACDEA010000000
VERSION     DACDEA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.100"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV136_002473"
     CDS             complement(<1..142)
                     /locus_tag="IV136_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8466416.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV136_002474"
     CDS             complement(183..437)
                     /locus_tag="IV136_002474"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8466417.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV136_002475"
     CDS             complement(548..802)
                     /locus_tag="IV136_002475"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8466418.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV136_002476"
     CDS             complement(995..1270)
                     /locus_tag="IV136_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8466419.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV136_002477"
     CDS             complement(1242..2195)
                     /locus_tag="IV136_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8466420.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IV136_002478"
     CDS             2807..4300
                     /locus_tag="IV136_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8466421.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IV136_002479"
     CDS             4435..4731
                     /locus_tag="IV136_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8466422.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IV136_002480"
     CDS             complement(4755..5714)
                     /locus_tag="IV136_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466423.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IV136_002481"
     CDS             5897..6748
                     /locus_tag="IV136_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466424.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IV136_002482"
     CDS             6769..6981
                     /locus_tag="IV136_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466425.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACDEA010000100         7319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDEA010000100 DACDEA010000000
VERSION     DACDEA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.101"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..779)
                     /locus_tag="IV136_002483"
     CDS             complement(27..779)
                     /locus_tag="IV136_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8466426.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1143..3809)
                     /locus_tag="IV136_002484"
     CDS             complement(1143..3809)
                     /locus_tag="IV136_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8466427.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4150..5601
                     /gene="cls"
                     /locus_tag="IV136_002485"
     CDS             4150..5601
                     /gene="cls"
                     /locus_tag="IV136_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8466428.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="IV136_002486"
     CDS             complement(5930..6745)
                     /gene="thiD"
                     /locus_tag="IV136_002486"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8466429.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            7039..7314
                     /locus_tag="IV136_002487"
     CDS             7039..7314
                     /locus_tag="IV136_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466430.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2148 a         1527 c         1249 g         2395 t
ORIGIN      
        1 tataataaaa tcataaaaag aacccttcac gagtcgtgaa aggttctttt tttatctgcg
       61 gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg tttttcactg
      121 aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc
      181 ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt tttcttcact
      241 ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg cgatgtatta
      301 ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc atcgattgat
      361 tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt ttctaattga
      421 ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt ctcatttgat
      481 tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc atttttttct
      541 gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc atagcgatta
      601 atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc tgctgtttca
      661 gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat agttacttgc
      721 gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt ttcattcatt
      781 tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca ttctcctctt
      841 ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc tatcataaat
      901 aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc
      961 aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt ccttaaacgg
     1021 tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
     1081 tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct cgttgattcg
     1141 ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat caaaacaatt
     1201 gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc tgatgggata
     1261 gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct acatacaact
     1321 acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt ttgtgtgttt
     1381 gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa tgacatggct
     1441 acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac agcaatccca
     1501 atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat tcctgaattt
     1561 ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc taatgcaatt
     1621 gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt aaatggattg
     1681 gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat
     1741 agataagaaa tagctttctt gatattatca aacacattac gtcctacttc tactgcgctg
     1801 acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt tgccacatct
     1861 gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc gtcatttaca
     1921 ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac gatacgaatt
     1981 ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc gtttagttga
     2041 tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc
     2101 ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat catgattgtt
     2161 ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg tggaggatcg
     2221 atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa
     2281 ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga aaaagcttca
     2341 ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc accatttagc
     2401 aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa caagtaattt
     2461 ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa aggaagttct
     2521 gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt ttcactaaag
     2581 tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact gatcgatgca
     2641 tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg
     2701 tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac tgtcattttg
     2761 ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc aagtgtttca
     2821 actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt tcccaaagat
     2881 aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc tacggctacg
     2941 gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc tccaagataa
     3001 atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc caaacttagt
     3061 tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt tgttgattcc
     3121 aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac taagaattta
     3181 ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc tccgattggt
     3241 accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc tgtcagcata
     3301 ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg tacataatcg
     3361 cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat cgttttttcc
     3421 ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa
     3481 ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa tactgcaaaa
     3541 ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac aatcagcaat
     3601 acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg cttcttcggc
     3661 ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg cgtggtgctc
     3721 aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc tttatagacc
     3781 gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga tcttggccct
     3841 agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag gtctcaccat
     3901 ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg caacaaacgt
     3961 tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt attaacctac
     4021 aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg aaaagtatat
     4081 tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta tgtaaggagg
     4141 aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc acttattgct
     4201 ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg gctcttagta
     4261 ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg tggattaacg
     4321 gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa ctttcagtct
     4381 tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga caaacagcaa
     4441 tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa ttctgtcact
     4501 cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa aaaagccaaa
     4561 cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca gcaagtctta
     4621 catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata tgatgcattt
     4681 ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc
     4741 catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta ccatgatcac
     4801 cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa tattgccaat
     4861 caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat acggattttc
     4921 ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt ctcggtacct
     4981 gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga tcgagatgaa
     5041 tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa cgaaagggaa
     5101 caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt ttggatacag
     5161 acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc aactgcctct
     5221 ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat ttatcgagca
     5281 acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt ctatgagaac
     5341 ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt aggttcagca
     5401 aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct
     5461 gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg ttcatccatg
     5521 acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca aatttctcga
     5581 ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac taagattaaa
     5641 gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg ttctcctgcc
     5701 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaacttgt
     5761 tccttgtgac tcggagctgg acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
     5821 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagaagctg tttttacttg
     5881 gctgttctta ttacttgatg cagggcaact ttttcacaac ttctttcttt tactctaatt
     5941 caccagctgc ccaatgattg atggggccat atttgtgccc tacatttatt tcttcacgaa
     6001 ttgcacggtt tacatagtct tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg
     6061 ctaactcagc tgcaatacat gcagatagcg aatcgccagt tccgttgatc cggtcggtag
     6121 gataaaatgg tgcatttaac caaaatgatt ttcctgattc gaacaatacg tagtcattga
     6181 ctgtttcttg ctgcgcattt ttagcatgat gtcctttgat cataacgttt tttgcaccca
     6241 tatcttgcag ttcttttgct gcttgcacaa aatcttcttc agctgctaat gtccgtccag
     6301 tcagcttttc tgcttcataa aaattaggtg tcagtacttc ggccaaaggg acaagctttt
     6361 ctttcaagct ttgcaaagca gattcttcta gtaaaagatt tccatgtttc gttacgatta
     6421 cgggatccac gactaatggt ccaaaatcat atttttggta attttttaca actgtctcaa
     6481 tcaactttga atctgctagc atacctgttt tagctgcacg tatttgaaag tccttcgcaa
     6541 tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt cacacttgct ccgattccgt
     6601 aagaattacc tgcaacacat gcggtcatga cagaaacacc gtaaacattt cttgtaaaga
     6661 atgtatgcat atctgcttgc atccctgcgc ttccatcgct atctgatcct gcgatcgtca
     6721 ttgcttgtac atactgatct tccatcaaat cgattcctcc atttttataa aaagattttt
     6781 cataatactg ttactgtttc tatcatatgt ttttcaaagt atccctcagt aaatatacca
     6841 ctaccttttc ttggtaatca aatttatttg aaaaaacatt tgcaataaat cagaaaatag
     6901 tggtcgttta tttgtattcg ctattctaat aataaataac cactatttga tttatatata
     6961 tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat atgaaaaaaa
     7021 taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa gaaaatcaaa
     7081 cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga caggagtata
     7141 aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga agaaggttac
     7201 gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat gaaaaaactg
     7261 attgaaaaag aatttccaag aagtcataaa ctctatgtga atcagcaatt ttaacttct
//
LOCUS       DACDEA010000101         7174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDEA010000101 DACDEA010000000
VERSION     DACDEA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.102"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..1642
                     /locus_tag="IV136_002488"
     CDS             11..1642
                     /locus_tag="IV136_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8466431.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1645..2724
                     /gene="uxuA"
                     /locus_tag="IV136_002489"
     CDS             1645..2724
                     /gene="uxuA"
                     /locus_tag="IV136_002489"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8466432.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2798..4225
                     /gene="uxaC"
                     /locus_tag="IV136_002490"
     CDS             2798..4225
                     /gene="uxaC"
                     /locus_tag="IV136_002490"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8466433.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4309..4956
                     /gene="eda"
                     /locus_tag="IV136_002491"
     CDS             4309..4956
                     /gene="eda"
                     /locus_tag="IV136_002491"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8466434.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5076..6452
                     /locus_tag="IV136_002492"
     CDS             5076..6452
                     /locus_tag="IV136_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8466435.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6462..>7174
                     /locus_tag="IV136_002493"
     CDS             6462..>7174
                     /locus_tag="IV136_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8466436.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDV"
BASE COUNT         2464 a          941 c         1477 g         2292 t
ORIGIN      
        1 gaggttaatt atggtttcga ttaaaaattg gcaaaattct atcgaagaat tcaatgaaat
       61 gcagattcta ctgccgtgtt ttgatgtcaa agaagttaag caagaaggcc agcaacatcc
      121 agtatggctt cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca
      181 atgcttagca gatcaaaatg aattagaaac aggagtagtt gtgtgtgaaa catttgatga
      241 agaagtagtg gaaaaagctt atcatgacta taacaatgac attttggaaa ttgttatgag
      301 tggagatggt gaactcaaaa aaagactttt ggcagtaacg gctgatagtt tatattgcaa
      361 tccgaagaat atagcggact atcaaagagt ggttaaatat tttgaagatc ctaaattaca
      421 gtttacaacg tttactatta ctgaaaaagg ttacagttta aaagattcta aagggcaatt
      481 cttttcggta gtcaaagctg atatcaaatc tggacctagc aatcctcaac atacgatgag
      541 tattgtaaca tccttgttat atagcagatt taatgctgga gagttgccga ttgcgatggt
      601 ttctactgat aatttctcac agaatggtca acgttttcaa gatagtattt ttacgattgc
      661 agaagcttgg caaaaagaag gatatgtttc tgaaaaattt attgcttatt taaaggataa
      721 agaaaaagtg acttttcctt ggagtatgat tgatcgaatt acgccaaatc cttcagagaa
      781 tgtaaaggaa gatctattga tgatggggct cgaggattta gatatcattc acacaaaaaa
      841 acataccaat attgctgctt tcactaatac tgaggtagtt cattatttag ttattgaaga
      901 tagttttcca aatggccggc ctaaattgga aaaggctggt gtgattatga cagatagaga
      961 gacggttgat aaagcagaca tcatgaaggt tacgacaagc ttgaatccac ttcatactgc
     1021 tttggcagta acaggttgtc tgctaggata tcaatcaatt tcagcagaga tgcaggatga
     1081 tgatttggtt ggtttaatca aaggaatagg ttatttagaa ggtttaccag tagttgaaaa
     1141 tccaggcatt attgatccaa aacaatttat tgatgaagta attaataaac gtttgcctaa
     1201 tccctatatt ccagatacac cacaacgaat tgcaacagat acttctcaaa aagtagcgat
     1261 tcgctatggt gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac
     1321 atttattcct atcgctattg ctgcttggtt acgttattta ctggcagtcg atgatcaagg
     1381 ggaaaatttt gtgccaagtc ctgatccgtt attggtagag ttgcaggata aattatcagt
     1441 attgaaatta ggtgaacaag atccttcaaa gattcattgt gcggtacagg aaattttaga
     1501 aaataaagaa atctttggtt tagatttata tcaagttggg gtaggaaaga aaactgaaaa
     1561 aatcttttgc gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat
     1621 aacagaagtt ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa
     1681 gaaaaaatct cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca
     1741 ttaatggata taccagccgg agaggtttgg ccgagagaac gaattaaatc tttaaaagat
     1801 gaagtcgaag ccaatggact taccttgaaa gttattgaga gtgttaatgt tcatgatgat
     1861 atcaaaattg gtttaccaac acgagatcaa tacattgaga attataaaca gacgattcgc
     1921 aatttggcag aatttgggat tgaggttatc tgttataatt ttatgccagt atttgattgg
     1981 gtaaaatcgg atttggatta tcgtttagat gatgggtctt ctacgttagc ctatatttca
     2041 aaaaatatcc ctactgatcc ggaggaaatt attagagaag tagaaaattc tactaataat
     2101 tttgaattgc cgggttggga acctcatcgg ttatcacaaa ttaagcaatt attagaagcc
     2161 tataaagaag ttgatgaaga gaaattacga gagaactttg catattttct caaagcaatt
     2221 atcccgacat gtgaagaagt tgggattaaa atggcagttc acccagatga tccaccatat
     2281 tccattttcg gattacctcg tattgttaaa aatcgtgacg atttagattg gttgtgtaat
     2341 gtagtggatt ctccttctaa tggtattaca ttatgtactg gaagtattgc agaacgacca
     2401 gataatgatg tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca
     2461 catgttcgta atatcaaata tttaagtgac aatggactcg atttttacga agcgccgcat
     2521 aaatcagaat atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga
     2581 tttaatggct atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct
     2641 ggttatggat tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg
     2701 cttgaaaagg gggcgagtaa ataattatat aagggagttt atttagagga aaaattttct
     2761 cttctaaaaa ctaacggaag gaaaggagtg tttaaatatg tttttagata agaacttttt
     2821 attagagaat gaaatagcta aaagactttt tcataaattt gcaaaacacc aaccaattat
     2881 cgactatcat tgtcatttag acccagcata tttgctagaa aataagcaat acaagaattt
     2941 gactcaggta tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg
     3001 agctaatggt atagaagaag agttgatcag tggtccaggt gatgattatg aaaaattttt
     3061 agcttttgta aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt
     3121 ggaattacaa cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat
     3181 ttggcgacgt gctaacgaaa aaattcaaac ggattcctat cgcccgaaag aattgttgga
     3241 gatgatggat gtgaaagcca tctgtacaac ggatgatcct acagatgact tgtggtatca
     3301 tactgagctt tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa
     3361 ggtctggagt atcaaatcat caggcttttc gaactatatt ttaaaactta gtcaaacagc
     3421 ggctatacca atcaaagact ttaatgggtt gaagcaagca ttgagccaac gagtggatca
     3481 ttttcatcaa attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat
     3541 agcagatgaa aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt
     3601 ccatttatca gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta
     3661 tacaagatat ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat
     3721 attaaaggaa caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt
     3781 gacacaaaat gttgtagcat tgttcgatac cgctgaaaag agtgggtgct taccaaaaac
     3841 aattttatat tcattaaatc caactgattg gcttccacta gcaacagcaa tgcaaagctt
     3901 ccaagggggt atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc
     3961 tgaaggaatg cgtcaacagt tgactacgat ggcacaacaa agtttgttgt cgaattttac
     4021 aggtatgttg actgattctc gaagttttct aagctatcct cgccatgagt attttcgtcg
     4081 agtattatgt caattactaa gcgagtgggt taaagcaggc cgaattccag atgatgaaat
     4141 gtatcttagt cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt
     4201 ttttgaaatc ggaagtagaa aataatactt gaaataaaag cttaaattta ttctttgtaa
     4261 gttagtacag ctagacaaag tatgtatcca taaaggaagg agatagatat gaaaaaagta
     4321 gaaattttaa gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg gaaaaataag
     4381 gatgaggcca ttaaagcaat aaattcaatt attgcgggtg gaattaaagg gattgaacta
     4441 acttttacag ttcctaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat
     4501 caaaaagatg taataatcgg agccggtacg gttttagatg cgtttactgc tcgtatcgca
     4561 attatggctg gggcagaata tatcgttagt ccatcttttg atcgacaaac agctgagatt
     4621 tgtaatttat atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag
     4681 gcacttactg ctggcgtaga tatcattaaa ctattcccag gttctgcttt tggtccatcc
     4741 attgtaaaag cctatcaagc tccattacca cacgtaaata taatgcctac tggcggagta
     4801 agtatagaga acttagaaga ttggtttgaa gtaggagttg ttgcagttgg tgtaggtggg
     4861 aatttattgg cttctgttga aaaaggtgat tttgaagaag taagtcgagt agctagtaaa
     4921 tatatagata agttttatga gataaagaat tattaggaga aaaaacaaat aatactctga
     4981 aatgtaaaaa ataaatactg actctttacg cttcagagta tagtacgtac tttcatcaga
     5041 atgaaaacgc ttaacaacag aagggagaga attgtatgga aagaaaattt ggtataagag
     5101 acaaaatagg atatatgttt ggagattttg gaaacgattt tactttcatt tttgctagtt
     5161 cttttttaat gatcttttat acaaaagtat tggggataag cggagctgcc gttggaacat
     5221 tatttttagt agcaagattt atcgatgcag ttactgatgt aactatgggg cgtattgttg
     5281 atagatcgaa agcaactaag cagggtaagt ttaaaccgtg gattttaaga atgagtggtc
     5341 cagtcgcttt agcaagtttt ttaatgtatc aaacttctat gatttcagct tctatgagcg
     5401 ttaaggtagt atacatgtat gttacgtata tattatgggg gagtattttt tacacatcta
     5461 taaatattcc ttatggatca atggcatcag ctattacttc tattacagaa gaaagaacaa
     5521 tgctatcgac attccgaaat gttggagcta ctttagcaag tttggtcatt ggtgtattaa
     5581 caccattatt tatttataca agagatgatt caggcgctca aatagttcgt ggtggatcaa
     5641 cttttacaat tgttgcggga atattttcga tatgtgcact tatttgttat ttaatatgtt
     5701 ataaactaac tactgaacga gtaagaattg accaatctca atacaaagtt tcattgttaa
     5761 aatctttaaa agggttagtt aaaaataggg cattactttc tataattggt gcggctattt
     5821 tcttactcct tgctcaatta ttaattatga gtatgaataa ttatgtattt cctgattact
     5881 ataatgatgc acagggaata gttttaatga attttatcaa tcctattttg gttttaatag
     5941 ttgtttcgcc tttaactttg tttttaagta aaaaatatgg aaaaaaagaa ctggcatcag
     6001 taggaatgtt cttttcagca atagtatatt tgatcttatt tttgataaaa gctagtaata
     6061 tgtatgtatt cttaattctt agttcggttg gctatatggg tttgggtgtg tttaatactg
     6121 ttatttgggc aaatattact gatgttattg atgatcaaga agtaaagtct gatcaaagag
     6181 aagatgggac cgtatatgcg gtatattctt ttgctagaaa aataggtcaa gctctagctg
     6241 gaggcgtcgg aggttggact ctttctatta taggctatga ctctttagca aatgtacaaa
     6301 cgggagatgt tttagaaaag ttatataacg cttcaacatt aattccatca atttgttttc
     6361 tgattgtagg attaatttta tttttccttt atccattatc taaagataaa gtcttagaaa
     6421 attgcgagct tttaaaacaa aaacataaat aggagagaaa tatgaataat tggatatttt
     6481 gtattgattc agatggctgt gttatggata cgatgactta taaacacaat ctctttttcg
     6541 gaccaattgc tgcagagatt tttgatgttc atcatagaga aaaattccta caggattgga
     6601 ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt ttagttaaaa
     6661 cattagaaaa aaatgatgta acatcgatta atcgattaaa gcactgggta tctctaacaa
     6721 aagaattgtc taatccatct ttgaagaggg aaatagaata ttctcagtca gaggatttat
     6781 taaaagcttt aaaatggtcg gaagaagtca atcaacagat tagtcaagca aaaggattag
     6841 ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat gggaagataa
     6901 ttattgtaag ctcagctaat aaggaagctg ttcaagaaga atgggaaaga catgaattat
     6961 tatcattagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagat attattcgaa
     7021 gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat tctcctggtg
     7081 atctagaagc tgcaaataaa aataaagttt tcttttatcc tattttggtc aataaagaaa
     7141 aagaatcatg ggaaaactta agaactgatg tttt
//
LOCUS       DACDEA010000102         7169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDEA010000102 DACDEA010000000
VERSION     DACDEA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.103"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="IV136_002494"
     CDS             complement(581..1597)
                     /gene="tsaD"
                     /locus_tag="IV136_002494"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8466437.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="IV136_002495"
     CDS             complement(1594..2058)
                     /gene="rimI"
                     /locus_tag="IV136_002495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8466438.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="IV136_002496"
     CDS             complement(2065..2607)
                     /gene="rimI"
                     /locus_tag="IV136_002496"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8466439.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2591..3415)
                     /gene="tsaB"
                     /locus_tag="IV136_002497"
     CDS             complement(2591..3415)
                     /gene="tsaB"
                     /locus_tag="IV136_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8466440.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3504..4484)
                     /locus_tag="IV136_002498"
     CDS             complement(3504..4484)
                     /locus_tag="IV136_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8466441.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4508..5992)
                     /gene="galT"
                     /locus_tag="IV136_002499"
     CDS             complement(4508..5992)
                     /gene="galT"
                     /locus_tag="IV136_002499"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8466442.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6004..6993)
                     /gene="galE"
                     /locus_tag="IV136_002500"
     CDS             complement(6004..6993)
                     /gene="galE"
                     /locus_tag="IV136_002500"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8466443.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1963 a         1559 c         1267 g         2380 t
ORIGIN      
        1 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
       61 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      121 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
      181 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
      241 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
      301 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
      361 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
      421 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
      481 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
      541 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
      601 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
      661 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
      721 cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
      781 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
      841 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
      901 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
      961 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1021 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1081 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1141 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     1201 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     1261 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     1321 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     1381 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     1441 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     1501 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     1561 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     1621 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     1681 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     1741 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     1801 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     1861 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     1921 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     1981 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2041 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2101 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     2161 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     2221 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     2281 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     2341 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     2401 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     2461 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     2521 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     2581 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     2641 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     2701 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     2761 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     2821 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     2881 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     2941 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3001 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3061 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3121 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     3181 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     3241 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     3301 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     3361 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     3421 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     3481 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     3541 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     3601 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     3661 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     3721 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     3781 tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca
     3841 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     3901 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     3961 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4021 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4081 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4141 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     4201 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     4261 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     4321 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     4381 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     4441 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     4501 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     4561 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     4621 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     4681 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     4741 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     4801 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     4861 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     4921 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     4981 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5041 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5101 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     5161 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     5221 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     5281 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     5341 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     5401 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     5461 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     5521 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     5581 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     5641 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     5701 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     5761 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     5821 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     5881 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     5941 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6001 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6061 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6121 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     6181 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     6241 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     6301 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     6361 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     6421 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     6481 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     6541 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     6601 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     6661 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     6721 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     6781 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     6841 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     6901 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     6961 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7021 tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac
     7081 aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt
     7141 caaggatttt ttgcaaaggt ttacaaaca
//
LOCUS       DACDEA010000103         7137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDEA010000103 DACDEA010000000
VERSION     DACDEA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.104"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..484)
                     /locus_tag="IV136_002501"
     CDS             complement(<1..484)
                     /locus_tag="IV136_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8466444.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     I"
     gene            complement(486..1520)
                     /gene="sppA"
                     /locus_tag="IV136_002502"
     CDS             complement(486..1520)
                     /gene="sppA"
                     /locus_tag="IV136_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8466445.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1553..2968)
                     /locus_tag="IV136_002503"
     CDS             complement(1553..2968)
                     /locus_tag="IV136_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8466446.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(2937..5090)
                     /locus_tag="IV136_002504"
     CDS             complement(2937..5090)
                     /locus_tag="IV136_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8466447.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5348..5584
                     /locus_tag="IV136_002505"
     CDS             5348..5584
                     /locus_tag="IV136_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8466448.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5603..5818
                     /locus_tag="IV136_002506"
     CDS             5603..5818
                     /locus_tag="IV136_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8466449.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(6521..>7137)
                     /locus_tag="IV136_002507"
     CDS             complement(6521..>7137)
                     /locus_tag="IV136_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8466450.1"
                     /translation="KYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFT
                     TTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVF
                     SIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRAC
                     LVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT         2250 a         1289 c         1063 g         2535 t
ORIGIN      
        1 aaattttttg accatagtta atagcttcta atgaacgaat aacaaccact gacgtgttag
       61 aaaaataatc cccaatatgt tgtttcttgg gcgtgaaagc agccaccaaa tacccaattt
      121 gaaatacaaa attttttaat ataaacctac atatacactc acgaataaag atagtttgcc
      181 aacttagctc tttttcctca aagctcacta ctcttatccc aaaaatcatt ttcccaattg
      241 tttgtcctga attcagctta gtcaaaagta caaaataggt taaataaata gctaatgacg
      301 ctataccata aaaagtaata atggatttgt cagattccag tcccatgatt ttgtaaaaga
      361 gacctatagt agcatgggtg atgatgctaa tacaaattaa atcaataaga aaagcaaaaa
      421 atcgtaacca aaatcccgca aatacataat taggcaaagt ttcaatgcca ctcttcttat
      481 tcattttata gccctccata gtaatacatt ggttttggag tatcagaaaa tccaagtttt
      541 tcaaaaactg aaattacacg atctgatgta gttgccttta gcccttgcca ttcagcaatc
      601 ttattaccta accaagaaga agcaaagcca gttgtgtcat ttttatattc tataaccgtc
      661 gcatcagtta atttcttctc tttttttagg gaatctaatg catcttcaga gtaaccaatt
      721 tgatccacca acccattttc tactgcttgg ttaccatcat aaattcttcc atccgcaagt
      781 tctttcactg cattagtatc catatttctt ccttttgcta caacattgac aaaacgatca
      841 taagcactca tcacaaattc ctgtataact ttattctctt cctctgaagg tttgtgttga
      901 ggtgacatca tatcttttaa tgcaccactt ttatacgtcg catcagttac gccaagtttt
      961 tctaataagc cagaataatt taaaccagaa ataatgacac caattgatcc agttgttgtt
     1021 tcttccgtag caaatatttt atctgaacca gcagaaatgt agtaccctcc acttgccgct
     1081 gtatttttaa atgcagtata gataggaata tctagctttt taatttttgc catttcttta
     1141 gcaatctcag cactttcata tattcctccc ccaggtgaat ttacttctaa taacactccc
     1201 ttaaccgctt tgtcttcttg aattttcttt agctgtgtta aaaaattctg atggttatat
     1261 tgttccctac taaaaagatt gctttcacct gtatctgcaa taactccatt gacggatagt
     1321 ttgacaatct ttttatttga tgcgccttct tctaacgttt cttctgtgat ttcgttagat
     1381 ccgtaaaaaa tcttatttat tcctgttagg gttgtatttg atgcttcctc tttatctttt
     1441 cctggaatta gcgcgacgat aatggatagt aacaataatc cgcacgctac tcccacagca
     1501 atccatcttc ttttattcat ataaactcct cctatattct tagtaaaaat aattattttt
     1561 tatttagaaa cacatcgaca atttgttgcc aatatgtttt ttctcctaca attaaagaca
     1621 actctccagt taatccataa cgaaacggaa acgattgagt tgtttttaac gttcctttca
     1681 ctgcataaaa aacacctttc tcagtggcag tagaattttc tgaaatttca tctaaagttc
     1741 cgtcgatcgt aatcggagcc ccaccttttt gatccaattt aaaatgaacc ttcatccctt
     1801 ctttaatata ggtactttca ttcgcaggta aaagtgctgt aaatttcatt tttgattttt
     1861 cgtggatcgg ataaatttct gcaagcactg ttcctttggc tagttctgct tgtccctttg
     1921 tttcttcatt taaatgaacc gttccagata caggagcttt tagtattcct tttgaaagtt
     1981 gatcgtccaa acttttgata actgactggt tcttctcttg tttttcattt agctctgtaa
     2041 ctgtttgttt tacagttgtt attgcttgtt ctagctcttg ctttcctttt tccgtttgac
     2101 taattctgtc attctcagaa gtagttggtt taactaattt agctttttct gactgatact
     2161 gttctacttc tttttttagt tgctcaattt gttcatttat gcttgctgaa attgtcagtt
     2221 tcacttgatt tttttgatca tcagaaacat tatttagttg ttcttgccaa ttctcatagt
     2281 ttgccatcat ttcttttgaa aaatctttta atgattgatt attactccaa gctacctgta
     2341 cttgttgcca ttcttgtaat ttactatctg aactctcaat ttgtttttct agttgtcttt
     2401 ttgtcttttc gtatacttct aattgctttt gatcatttaa ttcactttgc ttcaaagcgt
     2461 agcggagtga ttctttttct gacaacatgc tctttaattg attactgtag ccaaaactgt
     2521 catccgttga aaacaaattt tttcctttac ttatactttc aataaatagc tgagccatct
     2581 ttatttgttg agtaatacgt tggttttctt gttctatctg agccttttca ttctctatta
     2641 aagaacaatc caatctaacc aacacatcgt ccttatgaac aaattgattt tcttttagga
     2701 agttttccat tattttagct tctaatggca cttgtaactt ctctattttt tcggggttca
     2761 attgagccgt tgtccgaata acaacctctt ttttagcaaa ggttaaaaac aatccaataa
     2821 tacataaaaa aagaattaca gggtataaca cccaacggta gaagctatga tgttgtttag
     2881 tatagatact agaatgttcg aaccaattat tattctgcat aaattgtttc cctctcctag
     2941 atttcccata atttttgata gataccatcg gcaaaacgaa gttcatcgtg cgttcctttt
     3001 tctaccaaat gcccttcatg gagaactaaa atttgatcac atgcttttgc tattggtaaa
     3061 tgatgcgcaa taaaaataat cgttttttct tttaaattga ggagataatt cataattgca
     3121 tgttctaaca aggtatctaa tccactagtt gcttcatcta aaattaatat gtccggattt
     3181 tttaataaag cccgtgctaa ggctaatcgt tgtcgttgcc ctcctgaaag attatttcca
     3241 ccttcttcca acaatgtctc gtaacgtaat ggctgttgat taataaattc atttagttgg
     3301 actatctcac aaacttctat tatttgttcg aaacttggtg ggctatctaa accgaatata
     3361 agattctcaa tgattgttcc actaaagaaa aatgactctt gaggcaaata tgtcactcga
     3421 tcgcgtaata tctgaaaagg aatgtctaag tgattaatgt ttccataatg gatcgatcct
     3481 tctgatgctt cataaaaacg aacaaataac tttgcaagag ttgattttcc agaaccacta
     3541 actccaacta tagccgtttt actgtgcgaa ggaattgtac atgaaatttc gttcaacgtt
     3601 ggggccttca tgctatacga gaatgaaacg ttttttacag tgatatcttg ttgaaataat
     3661 tgttcagaaa gcatatattc aaaagactgg ttcgattttt ctgaatcaat cgcgaaaatt
     3721 tcatttaaac gattatttgc aacttgagcc gtttgcattt tcacttgtaa attaataatg
     3781 ttttgtaaag gatctgtcaa aaatactagt aaagcattgt atgtgataag ttgacctaga
     3841 ctaattctat tatccataac aaaataagaa cctagccata aaattaatgc actgctaatt
     3901 aattgaatca aatgtttaat tccttgttga atattatcaa gcgtaaccgt acgaaatgat
     3961 tttttcatta atttaacaaa ttcatggtct atacggttat atactttctc ttcaccgcta
     4021 tatgctttaa ttgtttctat tcctttaaga ctttctatga tactagaatt tacagctgca
     4081 ccggctgcca tttcttccct gttcgatttt tcatagcttt tcacgaaagc gaatattgca
     4141 actacataaa aaggcaaaga gcatagagtg attaaaaaca acgtactatt ctgaatagct
     4201 aacgtaaccc caacaagcaa caccatacca atatctaaaa atattgataa cgtagcactt
     4261 gctaaagcat caataatctt actggcatca agaaaacgag aaataatttc ccctgatttt
     4321 ctcgtagcaa aaaaattcat tggtaaagtt aacacatgtt tgaaatactg aagcataatt
     4381 gccatactca ttcgctgtcc taaaataata agaagataac tacgactata ttcaaacaat
     4441 acccgaaata aatagacagc gatcaagcca atggaaatta tatttaaagt tgaacgtgct
     4501 tgattaggga tgaaaaaatc aagaatacct tgaaaataat atgagcttcc tatgccaaaa
     4561 aacgtaatga aaatcgatgc taaaacgatg tggaatacta atcctttttg tttccaaatt
     4621 ataggcaaaa aagaacttaa tccagctact ttttctttaa taggaatata agtttctcta
     4681 ggcataggta ataaaagaat acctgtccat tctttttgaa acgcttcaat cgtttttcta
     4741 ttttttccat tagcgggatc tgcaatccat agtacgtttc ccttcgtttt ataaattaca
     4801 acgtagtgca tatagctgtt atcaaccaat atatgtgcaa ttaatggtaa gataagatct
     4861 ctttctttcc agatctcact atctgcttga atagccatac aatcaaaaga caatttttca
     4921 aatgtctttt tcaatccaaa tgctgaagta ccctctaaat cagtcccaga taggtcccgt
     4981 aatttatgaa tcgggatatc agttttaaaa tattttaaaa tcatcgaaag gcaagcaaca
     5041 ccacaatctt tttcatcttg ttgctgcaca aaaggaaatg attttatcac ttatctaaca
     5101 caactccttc taaaaaattt ttatagaaaa attctcaata tagctttaat acagtgaaaa
     5161 tattgtctca taattccaca aaaactttga aaacagttct tgaacactaa ctttttcatt
     5221 cctgaatcaa atttattttg gaaattttac tattcaagaa taaacttgat aagtttcagg
     5281 aagattttta ctgttaatca aatacgtatg ttacagtagc accgtaaaaa atattattta
     5341 aagaagcgtg tttaaacatg caaaatgtaa aggaactaag tattgtggaa atgcacaaac
     5401 tgttggtgga ggcaataatg cttggggaaa attggggcaa gttgtaggtg gacttaccac
     5461 aggggcagta ggtggtgcag gtttaggaac agcaatttgc ggaccagctt gcggtgttgt
     5521 tggagggtta tatggagctg tcgctggagg tgctgcagct ggctgggatg ctcgaaaaaa
     5581 ataaaaaagg agaataagta gtatgcatat taaaaacaca aaaacaacat ttatattatc
     5641 ttcagaagag ttgaaaaata ttcaaggtgg gagtgccgtg ggagttttag gcactacttt
     5701 ttctggggca acagcaggag taaaactttg ttcagccgga ggaccttatg caatagctgc
     5761 ttgtggtgta ggtggagctt tattaggtgc tggattcagc atgtggaccg gtgcgtaaac
     5821 aattaaaaaa tatcaaacct caagattggt ttgtgtcgat tacgaacata ggtatgttct
     5881 ttatcttttc aaaagtttgg aatggacaga actttttact aaagcttctt gtgctagttt
     5941 ttataattat attaaatttt tatcttttat taaagtcaga taaaaattct ttggaaatgt
     6001 aatggtagag atatttttac aaaaggactt gcagcgctgt agttggtgct gcaggaggac
     6061 ctatttcggc cgttacagga ggtattggta atgtacttgt cggatgtgca ttaaagcctg
     6121 ttcctgcaag ttaaatttat aaaaacagga aagtaattat ctcaaatcct atcaagtaca
     6181 atcataggat tgcacttgac aggacaaatt ttttaactaa aaaatgaaaa acactttcta
     6241 aaaattagta gaatttgcca aatctttcgc atcttccacc atacatttaa ttttaattcc
     6301 cctttatggt acttaataaa aacaagaaaa gaactattca tttggccctt ttttggttcc
     6361 agaccataga aatattcctc cagtaactaa tattgctgtt atgcacgtaa ctaataacag
     6421 ctataaaaat tggtacaact ttaataggat tcagaaaaat ttgcactagt gaaggaatct
     6481 attttattag aaaaatgcaa tctccctaaa gaaaaaacca ctaaacagtg ctttctttca
     6541 acttctcctt aattttttgt gctttcccta aagtaaagtt tctggccaac tctgaatcaa
     6601 aatatagtga acgtttttta aaatctattt ctttaacgtt aaagatattt actaaacacg
     6661 ctcgactaat tcttgttaac ataggatatt tcttttcaag ctcactaatt cttccataaa
     6721 attcgtactg cccatcttta gtacacaatg ataatctatg tggtaatttt gaagcttcta
     6781 caaaataaat gtcatttata tcaaaagtaa atgtttgaga acctatggaa aaaacaaatg
     6841 cttgattcgt aacctgtttt gttgcatcta tcctttcttg cgctaataac aataactcaa
     6901 caatctcgtt tcgatactca tctagtgttt gatcttttgt tacaaatcct aacgtttcaa
     6961 ctcttctttt tattgttacg ggtaacatct catcatgagt agttgtaaaa atgatttttg
     7021 cttgaacatc atattttctg attacttctg ctaattctat accgttaact tcatgattta
     7081 aatcaatatc taagaaatat attccttgct ttggtcggaa ttgtttgagg tattttt
//
LOCUS       DACDEA010000104         6952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDEA010000104 DACDEA010000000
VERSION     DACDEA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.105"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..507)
                     /locus_tag="IV136_002508"
     CDS             complement(<1..507)
                     /locus_tag="IV136_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466451.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIR"
     gene            complement(606..956)
                     /locus_tag="IV136_002509"
     CDS             complement(606..956)
                     /locus_tag="IV136_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8466452.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1113..2642)
                     /gene="ahpF"
                     /locus_tag="IV136_002510"
     CDS             complement(1113..2642)
                     /gene="ahpF"
                     /locus_tag="IV136_002510"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8466453.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2652..3215)
                     /gene="ahpC"
                     /locus_tag="IV136_002511"
     CDS             complement(2652..3215)
                     /gene="ahpC"
                     /locus_tag="IV136_002511"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8466454.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3359..4021)
                     /locus_tag="IV136_002512"
     CDS             complement(3359..4021)
                     /locus_tag="IV136_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466455.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4205..5851
                     /locus_tag="IV136_002513"
     CDS             4205..5851
                     /locus_tag="IV136_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8466456.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5842..6924
                     /gene="uxuA"
                     /locus_tag="IV136_002514"
     CDS             5842..6924
                     /gene="uxuA"
                     /locus_tag="IV136_002514"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8466457.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         2221 a         1321 c         1293 g         2117 t
ORIGIN      
        1 tctgatgaaa ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg
       61 gaatagaagc atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa
      121 cagaatacta ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg
      181 gaacaagctg ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc
      241 aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc
      301 gatactccaa gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc
      361 aagcaaaaca agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt
      421 tagcacaatc gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg
      481 aatattttgt ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg
      541 aactagtcat tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg
      601 aaaaatcaac ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt
      661 ttatctaaat aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca
      721 caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat
      781 tgttcgaaag tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc
      841 agccaacaaa tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct
      901 acatacatag gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc
      961 agtcaccttt ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa
     1021 aaagcgatta ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt
     1081 tccgctctct gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct
     1141 cctaaagctg cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct
     1201 gtacaatcac cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact
     1261 atttcatttc ttggattcat ttcgatcgtt ccatctagcc aatccgtatt agggagtaag
     1321 ccgactagaa tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg
     1381 atataggaga gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg
     1441 ataacagtga cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat
     1501 tctggtaaga attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct
     1561 tcaacaccag aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg
     1621 tcacagtgtg ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg
     1681 atagaacgcc agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg
     1741 ttagctaagc caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc
     1801 atcataatgt cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga
     1861 ccttctgtgt aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg
     1921 ccgaaagaat cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca
     1981 ctactagctg ctggtccacc gccaattact aatacatcat aaggttcttt ttcaaacaac
     2041 tcttcttcaa tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg
     2101 ctactaaatt cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg
     2161 atttcttttt ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca
     2221 gctaatatat tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa
     2281 ctgtcaaaat gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt
     2341 ttcggtgccc gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg
     2401 agggggatac cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta
     2461 ggtgtgcgca tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc
     2521 ttcgtaaatt cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca
     2581 ataggagatt caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac
     2641 atatccacca cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt
     2701 tctttccatt ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga
     2761 atcttatcaa tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct
     2821 tgaacgattc cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca
     2881 tccaaaacat caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg
     2941 attttaccga tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta
     3001 gagcaagaaa agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct
     3061 tctaattcag taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag
     3121 ttccccataa gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca
     3181 ttaaattctt cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt
     3241 tatttataat tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa
     3301 taaagattac tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct
     3361 actgctggaa atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt
     3421 gtaagtgcaa tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga
     3481 gtaaatcata atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta
     3541 accagcggaa acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc
     3601 ttcctgcaat ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt
     3661 cgtcatttat tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg
     3721 tttgtgtgtc caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga
     3781 caaagcgagc attttctgca gaaacaagat taattttgga aacatacgta ccactttgag
     3841 gtatcgtata cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc
     3901 ggccgatagc aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt
     3961 cagcatagat aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca
     4021 tctttattcc tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca
     4081 atctggtatg tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag
     4141 cgatttttat tgtataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg
     4201 aaaaatgatt gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt
     4261 agaaatacct cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg
     4321 gatccatttc ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt
     4381 agcggatcta ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat
     4441 aattgaaagg gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag
     4501 tggagagttc gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga
     4561 ttgttctgaa cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt
     4621 tacttttgct attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg
     4681 accgattgca gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct
     4741 tgctgcgcta ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac
     4801 agataatttt tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga
     4861 atggcaaaag gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat
     4921 tgttgcgttt ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag
     4981 caaaatgttg gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa
     5041 taatattgct ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt
     5101 tcccaatgga cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt
     5161 agataaagca gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc
     5221 tattatgggt tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt
     5281 atcgctgcta gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg
     5341 tatcattgat ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa
     5401 tattcctgat atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta
     5461 tggtgagacg attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt
     5521 cataccattg actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa
     5581 accattcatc ccgagtccag acccactatt gacagacttt cagcataagc tttccgatat
     5641 tcaactgggt acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa
     5701 taaagaaatt tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat
     5761 gttcatcaag atggtagcag gaccgaatgc cgtacgaaaa acattacagg attacttaca
     5821 agaacatgga ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa
     5881 aagattacct tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg
     5941 atggatatac cagtaggaga ggtatggccc agggagagga tcaaagaact aaaagaagaa
     6001 attgagtctc gtgggttaac actaaaagta attgaaagcg tcaatgtgca tgatgccatt
     6061 aaaattggat taccttcccg tgagcagtat atcgaaaact ataagcagac aatccgaaac
     6121 ttggcagaat atggaataga agtcatctgc tataatttta tgcctgtatt tgactgggtg
     6181 aaatctgacc ttgactaccg cttagaagac ggctcatcca cgttagcttt tatatctgat
     6241 gatattccag atgatccaag agaaatcgtc gcgcgcatcg aacattccag caatgaattt
     6301 gaattgccgg gatgggaacc tgagcgctta gctcatatca aatccttgct tgaagcctat
     6361 gcatcagtag acgaagaaaa acttcgagaa aattttgcct attttctcca atcaatcatc
     6421 ccaacatgtg aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc gccatactcg
     6481 atttttggat tacctcgaat cgtaaagaat cgagaagatt tagattggtt gtgccgcata
     6541 gtagacagcc cttataacgg aatcactctt tgtacaggga gtattgcaga agatccagaa
     6601 aatgatgtat atgggatttt agcagaattt tgtcaaagag atcgaatccc atttgcacat
     6661 gttcgaaata ttaaattttt agctaatcga gatttctatg aggcaccgca taaatccgaa
     6721 tacggttcat tagacatgta ccgcatacta aaaacaatgt atgacaacgg atttaacgaa
     6781 tacatccgtc ctgatcatgg aagaatgatc tggaatgaga caggaagacc aggatacggc
     6841 ttatatgatc gagcattagg tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa
     6901 ggaaacaaaa gtaaagataa ttagtgaaaa aaactgtaat tttattatgt aa
//
LOCUS       DACDEA010000105         6858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDEA010000105 DACDEA010000000
VERSION     DACDEA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.107"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..1573)
                     /locus_tag="IV136_002515"
     CDS             complement(20..1573)
                     /locus_tag="IV136_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8466458.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1721..3009
                     /locus_tag="IV136_002516"
                     /pseudo
     CDS             1721..3009
                     /locus_tag="IV136_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(3135..4214)
                     /locus_tag="IV136_002517"
     CDS             complement(3135..4214)
                     /locus_tag="IV136_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8466459.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4317..4757)
                     /locus_tag="IV136_002518"
     CDS             complement(4317..4757)
                     /locus_tag="IV136_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8466460.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4814..5224)
                     /locus_tag="IV136_002519"
     CDS             complement(4814..5224)
                     /locus_tag="IV136_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8466461.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5404..5838)
                     /locus_tag="IV136_002520"
     CDS             complement(5404..5838)
                     /locus_tag="IV136_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8466462.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(5938..6786)
                     /locus_tag="IV136_002521"
     CDS             complement(5938..6786)
                     /locus_tag="IV136_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8466463.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2445 a         1260 c         1137 g         2016 t
ORIGIN      
        1 tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga
       61 gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt
      121 cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt
      181 tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga
      241 tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat
      301 cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat
      361 gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt
      421 gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca
      481 acacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt
      541 taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt
      601 tttcaggtat aaacaaatca aatacagtac aaaaggactc caccatttct cttgaataaa
      661 gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg
      721 attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac
      781 aaattggcag attaggtcgc aaaatatcta cagagggatc aataaaaaag tcaatatatt
      841 catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac
      901 atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt
      961 cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt
     1021 ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta
     1081 cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtaca
     1141 atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg
     1201 tttgctgact taaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct
     1261 gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat
     1321 caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta
     1381 gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag
     1441 acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga
     1501 aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt
     1561 cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac
     1621 tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc
     1681 ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt
     1741 aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga
     1801 ggagatagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt
     1861 tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc
     1921 gaccatcaaa gcaaaaattg actattcagg agacttagca cctatactac aatcagctgt
     1981 tacatttgag aatgttgatc ccgctttgtc gattggctat gacgagaaca ctttgactat
     2041 ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc
     2101 agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatc atttaacgcc
     2161 gtatagtcag cggcagacaa ttgagcaaga ttcagctatc gactcaggag gagatcttgt
     2221 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
     2281 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
     2341 accagagcca gacaatgaat caaaacctga aataacccct ggagaaaaac cagatgtaga
     2401 cccagaagaa aaaccagatg tgactccaga accagatacg gattctggaa accaaacagt
     2461 accggaaaca aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtagc
     2521 cccagaagaa aaaccagatg taactccaga accagatacg gattctggaa accaaacagt
     2581 accggaaaca aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga
     2641 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
     2701 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
     2761 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
     2821 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
     2881 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
     2941 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
     3001 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
     3061 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
     3121 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
     3181 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
     3241 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
     3301 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
     3361 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
     3421 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
     3481 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
     3541 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
     3601 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     3661 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     3721 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     3781 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     3841 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     3901 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     3961 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     4021 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     4081 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     4141 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     4201 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     4261 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     4321 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     4381 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     4441 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     4501 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     4561 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     4621 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     4681 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     4741 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     4801 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     4861 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     4921 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     4981 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     5041 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     5101 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     5161 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     5221 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     5281 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     5341 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     5401 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     5461 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     5521 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     5581 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     5641 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     5701 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     5761 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     5821 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     5881 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     5941 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     6001 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     6061 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     6121 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     6181 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     6241 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     6301 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     6361 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     6421 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     6481 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     6541 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     6601 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     6661 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     6721 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     6781 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     6841 tcaccttaag ttaacttt
//
LOCUS       DACDEA010000106         6782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDEA010000106 DACDEA010000000
VERSION     DACDEA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.108"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IV136_002522"
     CDS             complement(45..1511)
                     /locus_tag="IV136_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8466464.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IV136_002523"
     CDS             1898..2494
                     /locus_tag="IV136_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8466465.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IV136_002524"
     CDS             complement(2552..3034)
                     /locus_tag="IV136_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466466.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IV136_002525"
     CDS             complement(3140..3337)
                     /locus_tag="IV136_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466467.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IV136_002526"
     CDS             3632..4390
                     /locus_tag="IV136_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8466468.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IV136_002527"
     CDS             4450..4878
                     /locus_tag="IV136_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466469.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IV136_002528"
     tRNA            4924..4996
                     /locus_tag="IV136_002528"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IV136_002529"
     CDS             complement(5146..5517)
                     /locus_tag="IV136_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8466470.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IV136_002530"
     CDS             5732..5917
                     /locus_tag="IV136_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466471.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IV136_002531"
     CDS             complement(6000..6188)
                     /locus_tag="IV136_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466472.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IV136_002532"
     CDS             complement(6212..6475)
                     /locus_tag="IV136_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466473.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..>6782
                     /locus_tag="IV136_002533"
     CDS             6633..>6782
                     /locus_tag="IV136_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8466474.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSF"
BASE COUNT         2243 a         1318 c         1090 g         2131 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacaactg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 tt
//
LOCUS       DACDEA010000107         6708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDEA010000107 DACDEA010000000
VERSION     DACDEA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.109"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1442
                     /locus_tag="IV136_002534"
     CDS             <1..1442
                     /locus_tag="IV136_002534"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8466475.1"
                     /translation="SLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEY
                     LFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTI
                     TEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTL
                     DQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGV
                     DAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAP
                     KSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPA
                     NLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERK
                     DVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKK
                     LLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1432..2649
                     /locus_tag="IV136_002535"
     CDS             1432..2649
                     /locus_tag="IV136_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8466476.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2662..5811
                     /locus_tag="IV136_002536"
     CDS             2662..5811
                     /locus_tag="IV136_002536"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8466477.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2345 a         1101 c         1365 g         1897 t
ORIGIN      
        1 aatccttaga ggaatatgac acagtaagca aacaaacaat gttgtatcgt gaattacttt
       61 cagatgaaga atcaaaagaa gatctgattg ccacaatcgt tgatattttg ggatatgcaa
      121 ttgcaccaga gtatcttttc aacgtcttag cggatcaagc aaaacaagca acgttccaat
      181 tgaacgatct aaacaaagcc tttgtgcaat tggcatctac ctacaatcaa tttaatgggt
      241 tatttgatga tgtggatctc caatcaaaaa aactaggaac agatgagcaa caacgaaatg
      301 tgacgattac cgaagtcatc aagaaattga atgatgtaga tgttttagaa cacgatgggg
      361 acgtgatcgg ggatgcgtat gagtttctga tcagtcaatt tgcttcagaa gcagggaaga
      421 aagccggaga attttacaca cctcatatgg tttctgatat gatggcgcaa atcgtaacgc
      481 tcgatcaaaa ggaacgacga ttctttagtg tttttgatcc aacgatgggt tcaggttcat
      541 tgatgttaaa cgtacgtaac tacttaacac acccagataa cgttaaatat catgggcaag
      601 aactaaatac aacgacatac aatctagcaa aaatgaactt gattttacat ggtgtcgacg
      661 ccgaagaaat gaatctccgc aatggggata cgttgaataa ggactggcca acagatgaac
      721 cttacacatt tgatgcagta gtaatgaacc caccttattc ggctaattgg tcagcagata
      781 cgactttctt agatgattct cggtttaatc gttatgggaa attagcgcca aaatccaaag
      841 cagattttgc gtttcttctt catgggttct atcatttgaa ggaaacaggt accatggcca
      901 tcgtcttacc acacggagtt ttattccgtg gcgctgcaga aggggtcatc cgtcaaaaat
      961 tactagaaga tggcagtatt tatgctgtga tcggtatgcc tgcgaacttg ttctttggga
     1021 catcgattcc aacgacggtg attgtgttga agaagaatcg tcaaactcga gatgtattat
     1081 tcattgatgc tagtcgtgaa tttgttaaag ggaaaaatca aaataaacta tcggaagaaa
     1141 acattcaaaa aatcctcgaa acctacgctg aaagaaaaga tgtcgagaaa tacgctcatt
     1201 tggcaacctt tgatgaaatc aaagaaaatg actataactt aaatatccca cgctatgtag
     1261 atactttcga agaagaagaa ccaattgata tggttcatgt cggaaatgac atcaagaaga
     1321 tacgacaaga acagcaagtg ctagaaaaaa aacttttaga agctatctct tccctgcaaa
     1381 caacacctga aaatgaagcg tggttacaag gtgcgctgga ggtgttcaaa catgagcaat
     1441 gatacacaac cagaaatccg tttcccagga tttacagatg attgggaaca gaggaagttg
     1501 ggggacttcg ttgtagatta tgttgaaaag acaagtgtac aaaatcaatt tcctatgcta
     1561 acttcatctc aacaaaaagg gatagttttg caagaagact attttgcaaa tcggcaagta
     1621 actacagaaa ataatattgg ttactttgta ctacctagag gatatttcac gttccgtagt
     1681 cgaagtgata atgatgtgtt tgtgtttaac cgtaatgaca ttattgatag agggattata
     1741 agctattttt atcctgtatt tactttaaaa tctgctgatt ccgacttctt cttaagacgt
     1801 attaacaatg gaatccaacg acaattatct attcaagcag agggaacagg tcaacatgtt
     1861 ctgtctctaa aaaagtttaa aaatatagta gcaatgtttc ctagtgagga agaacaacaa
     1921 aaaatcggca ccttcttcaa acaacttgac gacacgatcg ctcttcatca gcgtaagtta
     1981 gatttgctga aggaaacgaa aaaaggattc ttacaaaaaa tgttccctga aaacggagca
     2041 aaagtgccag aaatccggtt tcctggtttt acggaagatt gggaagagcg taagttggga
     2101 gaattaggta aaactcaatc gggaattgga tttcctgatg ccgagcaagg tggttctgaa
     2161 ggtactccat tttttaaagt ttccgacatg aataacattg gtaatgagta tgaaatgaga
     2221 aatgcaaatc attatgtatc taatgagcaa attgagcgta aaaaatggaa gccaataaaa
     2281 gatgtgccag cgattatatt tgctaaagtt ggcgcagcaa taatgcttaa tagaaaacgt
     2341 ttggtcacaa gccctttctt aatagataac aataccatgg cttatttatt taataatact
     2401 tgggatatct attttggtaa aatattgttt gaaactataa atttgccaag atactcccaa
     2461 gtaggggcat tgccttctta taacagttct gatattgaga atatcagtgt taaggttcct
     2521 gtaaaggatg aacaacaaaa aatcggtact ttcttcaaac agctagatga tacgatcgct
     2581 cttcatcaac gtaagttaga tttactaaaa gaaacgaaaa aaagcttctt acaaaagatg
     2641 tttgtctagg agggaagaag aatgaaaatt agtcaccgtg aggaagcaga agtcgaagaa
     2701 cagttgattc gtgtacttgg agaagggcat aatcagtgga cgtatcgtcc ggatctaaaa
     2761 tcagaagaag acctttgggt caatttacgc caaaaaatta tctcaaataa tcaagcagaa
     2821 ctgaatgatt ttcctttgac cgataaagaa tttgaaacga tcaaaacgga attactgctg
     2881 cgaacgaaaa ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg
     2941 atcacgattg aacgggaaga ttcgcaattg ggttctgtct cacttatcct ttattccaat
     3001 caagacattg gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga
     3061 agtaacatag aagcacggga tcgacgtttt gacgtcacct tattaatcaa tggattaccg
     3121 attgtccaaa tcgagctgaa acaagtaact gcaaaagatg gtgtttatca ggcatttaac
     3181 caaatcaaaa aatatgcaga agaaggtatg tttcggaaca atatcttttc gaccttacag
     3241 ttgtttgtgg tttccaatga acaaaccact cggtattttg caaatgcctt accgaaagac
     3301 ttgcacccta agtttttatt tagttggcga acgaaagaca atgaaaaagt agaaaactta
     3361 tatgagtttt gtaaacaagt gttaaatatc ccggatgctc atcgtttgat tgcggattat
     3421 acaatcgtca gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagta
     3481 catgcgattc aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat
     3541 gcgactggtt caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa
     3601 tcggggatcg atcgaacgat catgttagtg gaccgcaaag atttagataa tcaaaccacc
     3661 acagagttta caaaatttgc atcggaattt aatactggga tttcttccgg gaacgccaaa
     3721 gcgaatagct tgatcgttgg aacgggaagt gccaaagaat taagtgaaac cctgttggca
     3781 gatgccaatg caaatgttgt aatcattaca acccgtcaaa aactagatgc ggcgttgaaa
     3841 tatgcaaaga aacaagaaga gaaaaaagga accaatcgct tccaaaaatt aatgggccaa
     3901 catatcgtgt ttgtagtaga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa
     3961 atcaaaaaaa tgtttccgaa atccacttgg tttggcttta caggtacacc gatttttcct
     4021 gagaatcgga aacaagccaa agggcaattg gcacgtacaa cccatgatca gtatggagag
     4081 gtcttgcata cctataccat taagaatgcg ctagaagatg gctctgtctt aggatttcaa
     4141 gtagagcatg aaaacaccgt ggagccgact tcactagaga ataaaatcta tcggaaatta
     4201 aaagaagtag aaacatatgc agagtattca tcagaacaga tcaatcgtat gattgatcaa
     4261 atggaacctg tcaaaaaaga aagttatttg gacccagctg tttttgaagc agatgaacat
     4321 atccaaaaag tcattcataa aatgttccgt ccagataatg cctatacaaa atttgatttt
     4381 cgaaatggca ggccaacgaa atctgcaatc ttaaccacga gttccattga catggccaaa
     4441 aagtattatc gggccatcaa agagatgacg aaagagcctg attggttaac aaaagagttt
     4501 cccaatcagc tcattcgtga aggacgaacg atggaggatc ctgattttcc acgaatcgct
     4561 attacctatt cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa
     4621 aaaattattg aggaatataa tcagtattat gatacttcct ggtcattagc agacattgag
     4681 cgttataacg gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt
     4741 gggaaacaaa tcgatttagc gattgtagtg gatcgtctat taacaggttt tgacgcccct
     4801 acgattcaaa ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagctttt
     4861 tcacgtacga atcgtacgta tcctgataag acaaaaggat tgattgtgac gtttcgtaag
     4921 ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac tgtattcaaa agcaaaagaa
     4981 gaatcaggtt tgatttatcc aagttatcag gaatccaaaa aaagattcaa acaagcatat
     5041 ggtaagttaa aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc
     5101 cgtatcgagt acgtcaaagc atttcaagaa ttgaatcgtg cttatgaatc gttagtgact
     5161 tatgacgatt acaatgatga catggtgaaa tctccaactt tgaacaatca aattcaactt
     5221 ttggaagaac aaattggcgt gtacgagacc gtgaagggtt cattaataga ggaagaacca
     5281 gaaaaagaag gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg
     5341 tatgatattg attcaactta tattgatcaa ttactaggaa cttatgctgc gaatagttca
     5401 gatgcacgag aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa
     5461 gaagtttacc gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat
     5521 atctttgtca ttaaacgtcg ctattttaca caagcgagtg atgatttgat tcatgtcttt
     5581 tccaatgagt ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatctacca
     5641 ggtgaagatc caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat
     5701 aaagccaaac atccagaagc gaagccattt aaatacccac aagaaatgaa gcgagcatgg
     5761 cgtaaaatgt tagatgatga gttgattcca cttgagaatg aattgaggta aacgagaaga
     5821 gttaagaaaa ttgtacgatt caaagatggt agtcaagtgg aagtgtagaa aatagggata
     5881 gatgtctttt atttgttcac attttataac gaagtattta agacagctat tgaataatgt
     5941 ttcctccacc tttttcattc atttacccca ccccgttgtt atatgaccgt gttttgagag
     6001 gaaccaataa aaagcatagg ctaactaggg gttccttttt cttttgtttt tttggaatcc
     6061 gcttaagtga aatgtatctg aaatgaaacg gatataaagt gattttttgc tataatgaca
     6121 aggttttagc tgtttcagat accatgtaac ccaaaatagt ttattgagaa aataggagag
     6181 tatttgatac taactagtag gaaaaaatga gataaaaatt aaatggaatc atttcaggta
     6241 atctcaaata aaaaaatata cagtcctgtg gcactttacc tatgggactg tatatttttt
     6301 actctcagcg ggcttgtttt gactccgtgt ctgactccgt cgtattgatt ttaaatgata
     6361 ttcgatgacc atttaacagg tcatttttga aatttttgtc tctatatgac tcagtttgat
     6421 aattattgat agatcaattt gggtctatat cgaacaaggg aataatccaa taaaaaatga
     6481 tttagaatat caaaataaca aaaaagagga ctacctgtat tcgtcctctt ttttgactag
     6541 attttgacca gtctaattta cattctaata tattcatatg agctaatttt ttgattaaat
     6601 ggtaagtttt tccttgtttg ttttagttta aactagtgtg taatttaaaa atctatatgg
     6661 aagggtctgt ggggtaatac ttaaataaag gctaggaacc tgataaca
//
LOCUS       DACDEA010000108         6671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDEA010000108 DACDEA010000000
VERSION     DACDEA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.110"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1083)
                     /locus_tag="IV136_002537"
     CDS             complement(<1..1083)
                     /locus_tag="IV136_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8466478.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIR"
     gene            complement(1224..1826)
                     /locus_tag="IV136_002538"
     CDS             complement(1224..1826)
                     /locus_tag="IV136_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8466479.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2071..2277
                     /locus_tag="IV136_002539"
     CDS             2071..2277
                     /locus_tag="IV136_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466480.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2589..3308
                     /locus_tag="IV136_002540"
     CDS             2589..3308
                     /locus_tag="IV136_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8466481.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3389..4072)
                     /locus_tag="IV136_002541"
     CDS             complement(3389..4072)
                     /locus_tag="IV136_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8466482.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4290..6476
                     /locus_tag="IV136_002542"
     CDS             4290..6476
                     /locus_tag="IV136_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8466483.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2466 a         1134 c         1257 g         1814 t
ORIGIN      
        1 acgaatcatc acggaatgat tcttcttgag tatggtcaat tcgatttttt tcttctttgc
       61 agcactagct ccttcaatcg cattgtccaa gataatacca agcatacgta caaggtttac
      121 cgtatgtttc gtatccagtt cgattggctc aggaaaatga agctggacga tcaacccttt
      181 ttcctgtgcc atcatgagtt ttaatgacag taaactacgg atctcaggaa ctttcatatt
      241 atccaacata cttagattgg ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt
      301 ttgatcataa taagaccgta atccttctaa gtcgccttct gaaatatagc tgtgaagaga
      361 aaaaagcaga ttttggtaat catgtttgaa ttctctcaag tcttgatagt ttttttctag
      421 atctttgata taatcctgtt gcatcctgac ttgttgctct aataactgat cagctaattt
      481 cttattagag attttccaat aataaaccaa gatgatcagg ccaacacaca gatatagaat
      541 gaaaaaactc gtattcaaag ctaagacatc aggtgtttca ccagaaaaac gggtataaaa
      601 aataatgata tagtatgtaa ataatatcaa tgtcccgatc gtaccgatca tacggcggtc
      661 cacagtgtga aaccaagtct ctaacgattt ttttaaacaa aaagctaata aagcagataa
      721 aaaagcggat aagacaatgt gaaaatactg caatgaatct cctggttcaa taaccgaaga
      781 tttcaaaaca aaaaaatcgc cgatgcttgt taagtgatct cctaaaagcg acgtcaataa
      841 cccaaaagga agcgcgtaaa ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag
      901 catcagcatt cctatagcaa ttagagcagt aaaaatccct aaaatcgaat agacccatcc
      961 aatcaaaata caagaaatca gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg
     1021 gtcaatcaat aagatacaaa ttgtgtaaat caaatattgc aacgctatgg accataaaaa
     1081 cataaagtac agctcccatt gatttttacc aaattatagc acaattggcg tattattttt
     1141 ttaatttttc agggattttg aattcagaac tcataccaaa gcagcagcca gatacaaaaa
     1201 agtcagataa gttagttaat tgtttaatga ttgttttttt catgaaagat tcctccaaaa
     1261 ttcatcaagt agtttgtaat aggaagcaac gtagctattt ctaaaacaat cccaagtagc
     1321 aaataggtag aaaagtaaga agctgagaaa aataacgtaa tgagagaaaa aattagaaac
     1381 atgctgatcg ccttctttaa taatggggga cgtttctcgg gttcaatggg attgattgct
     1441 gtacctcttg atccatagcg aagcagtaac aaaaagcagc tgattaacaa aaagaccatc
     1501 attgggaagg agatagtcaa ttgcagatgt ttcatcacca agggaacgcc ccataaataa
     1561 agtagtgtcc acagcagaca aacgaattca gagtcggaat gcaatccata agcatatctg
     1621 cggatgcaga tataagaaag aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga
     1681 gaaaagacta gaacgaccgc tagttttcca agattattca ataaaacttc taagacaaat
     1741 tttacttgga catacaccat atcttcttcc gatttgtctc cgctgaataa ggcttctaat
     1801 aatttgttca caactttatc tttcatctca tacgtacctc caccacaaat tttatgctaa
     1861 aaaaaataaa aaacgtcttt tgtttcataa aaagtggttt tgacatcaaa accgtcatga
     1921 tctcctaaaa aaatcttcgt tttccaagaa ccttaccaaa aaaagtgaca gttttgatac
     1981 gaaaagtact ttttatgaca agtttgtgct ttttcgagtg atttatgtta aaaaattact
     2041 gaccttataa aaggtgatgg agggattctt atggaaaatg aacaattgtt tgaagtcggc
     2101 gaaacatatg catttaaatc tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa
     2161 atctatgaaa atagtgtgat gatacgtgtc atgaaatgcg cagtagaaga caatgatacc
     2221 gctcataatc tgcactggca gttagttgta cgcaaatcaa gtacaataga agaataaaaa
     2281 cagataaaat gttgagagca taaagaggtt gtgagtctcc cgtttagctc tgagttacaa
     2341 ggaataatgt taaaaaacag cttttcatgt tttatgaata ttgccttgtg accgaggtgt
     2401 tggcaggaga atactgttta tttgaaaaaa gggctgcgac aagacttttg tcacagcctt
     2461 tattttcatg aataggagtt acttttggct acttttagag aagtagcttt taaggagcag
     2521 ataaagtcaa ggcttttatt tctataaatg aagtagtaaa atgaagaaca gattttatta
     2581 caggaaggat gccgataatt gtgatcattg ttgcaggaac gatcggtgca gggaaaagca
     2641 cactaactga aatgcttgcc caagatttag aaacaaaacc tttttatgag aacgtggaag
     2701 ataatgaagt attgccgcta ttttattcta atccagaaaa gtatactttt ttattacaga
     2761 ttttcttttt gaacaaacgc tttttggcaa tcaaagatgc tttcagccat gatgacaacg
     2821 tactagaccg ctccatctac gaagatagta tgctgtttca tttgaatgca gatttagggc
     2881 gtgtttctga agtagaagta aagcagtatg aaggtctttt agaaacgatg ctgaaggagt
     2941 tagaagaaat ttcgccacaa aagaaaccag atctactggt ttatattcgt gtatcctttg
     3001 aaactatgtt agcgagaatc aaaaaaagag gccgggaata tgaacagcta gaacaagatc
     3061 ctgaactcta ttcctactat aaagaactca atcgacgata cgaagagtgg tatgaacagt
     3121 ttgatatatg tcccaaaata gtgatcgatg gggacaaata tgatttcgta gccgatccgg
     3181 catgtggaga acaaatcgta caagaaatca agatgcgggc aaagaaaatg acagaagagg
     3241 cagatgccca gtcgatccgc tacactacaa ataacccgaa agcaaaggga atctttacaa
     3301 ctaaataata taaaaactaa gcaaattgct cactcgcaga tatttctctg tgagcagcat
     3361 cttgcttagt ttttttgatt cattgagatc aaatagtgaa ccgattcgta tgataaaatt
     3421 gccataaatg aaatgtctga gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa
     3481 tcgaatgggc gccaaacaga tgatgacaga aaacaaggac aatagctcct gcgataaagg
     3541 caagaacgac acttagatag tggaaaaatc gatcaaaatc tgctttttct ttagaaatac
     3601 aataatcata taagttcgtt gctacccctt ttaaatttcc agttgtaaat aaattagtgt
     3661 aggcacgccc gttgatttta tcaaaagcta cccactggat cgatgcagta aaagcaatca
     3721 aaatcgttac taatgcggga tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca
     3781 ctgcttcata atatagaaca aacaagcgcc agaaatgttt ttcttttttt ctaaaataat
     3841 caattagata ctttgttaga agaatcccta agaaaaagaa aagggtagac aatacttttt
     3901 taccaacact tagccattct ccgtcaaatg cttggatgat cgatagaatg atattgccag
     3961 tttgagcaga ggcaaaagca ccatattgga tataagtata actgtccatg gctcctccga
     4021 taaatgtcag caaaagtcct attgtacgcc cctcgtgaaa ggaagtctgc atttttttca
     4081 tcacctttta tgaatatttt tgtcttcctt atcttagcat aaacgattga aaaaaaggaa
     4141 aaattttcag aaaactaaaa tcatatcgct tcccattttc atttttagaa aggtctgtta
     4201 gtataaagaa aaaatagaca acgaaacttt aaataaccaa cttgaaataa acaaattcaa
     4261 agaagaaaga tgggaaggaa atcaatcagt tgaatgaaaa agaaattctc cgattacaaa
     4321 aagaattgaa acgctgggag aacaagtata aaaagcttca acaagtcttt cggctacaag
     4381 aagaaatcat tgctacctat gaagcggctt atggcgaagc acccggactt attgaggaag
     4441 aagtacaagt aaaagaaagt gaaggaaaca gctctttctt taaaatgatg gagaaacgtt
     4501 cctataatcg cttcaatcaa gaacaaaaag cagctatcac atatgatatg aagaaacatc
     4561 ttcgaatcat agcaggagct gggagcggca aaacgcaaac aatctgtgca aaagctgttt
     4621 atctgatgac gcaaaaacaa gtagacgaag aacgcatact aatgatcact tttacaagga
     4681 acgctgcaaa tgaactaaaa aaacgagtag ataatttcag ccagagaaaa accgctgtcc
     4741 atatcggaac attccatagt atctttttta ggatatacaa tgaaatttgt cgaaaatttc
     4801 ctgaagtagc aatgcaagga atacaaggag acttttcaaa ggatactgca caaaaagtga
     4861 atgctgtttt gcaacagctg atacgtaaat ataatcttta tatatttgat caatatggag
     4921 aaaaaacgat tgcttctcgg ttagactatt ggcagaatat gaacctttca gtagaagaaa
     4981 tggttcagtt agttaaagaa aaatatgatt ccattgataa gaatgcacga cagccgatat
     5041 cagaacgact gtatggattg ctcacggaac tccaagaaca aaaaagaagc cagcaattgc
     5101 tagagttcaa tgatgtactc caaaatctga aaagcgcttt ggagaatgag gagatccagc
     5161 attatatcgg ccaaaaatat gattatctat tcattgatga atttcaagat actaatccac
     5221 ttcaatggga aatcgtgaaa cggatgacaa aggaaaacag gatcaagttg attgttgttg
     5281 gagatgatga tcagagtatc tatggattca gaggatcaga gccagcatac atcaagaact
     5341 ttgaaaaaga atatcctacc aaaactcttt tcttattaac taattatcgt tctcgggcag
     5401 ctattgtcca aggagcaaat agattgatca gctataataa aaatgatcgg ataccaaagg
     5461 cgatgattcc agcacaaaaa gaagcaggtg ttatggaagc ctatttgttt tctgatacta
     5521 aagcagaaag tcagtggcta atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat
     5581 ataaggaatc aatcatatta taccgatcag cttcacaaac gcagcagctt gtccaatctc
     5641 tgctgaaaac gaatattcct tttgtactgg aagcagatag tccatatgaa ggtatctttg
     5701 gtatcaagtc tttccaacat ttctatcaaa aaattgttca ttggcaaaca gcactgaatc
     5761 tgcaaaagaa aaatcaggcc taccagcaat tgctccggca gctgatggca gactgctacc
     5821 tgaaaaaaag tgaatgcgat caatactttt ctcctaaata tcaaaatata gccgttgcag
     5881 attatatttt gtcgataagc cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg
     5941 aggaagcgtt gcgtaaaaca gaccagcatc aagaaatcta ttcgcttgtt caaagctatc
     6001 ttcatctgcc acgtatagct aaggaaatcg accagagtga tcaagaatgg atacgagaag
     6061 aagtaaagca gcatgctact ttcagaagac tccaagcctt atcgcaagaa acccaagcga
     6121 cagctaaaga attaaaaacc cgcttgattg cttacagaca aggaaaactt gatgcactat
     6181 gtatccagag cattcacaaa agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt
     6241 gtaacgaagg aagtttgcct tataacggag cgacagaaaa gaaagtaatc gactttaatg
     6301 aaataaaagc agaaccagta acgacgatag aagaagaacg aagactattt tatgttgcga
     6361 tgactagagc aagaaatcgt ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat
     6421 tgaaagtatc gagatttatc aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga
     6481 agagagggtg tgacagaagt ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc
     6541 ttctcaaatt tttgtgaaat ttcagcttat tttcgaagaa gctatttctg gaacacagtt
     6601 tattcggaaa agggaggtgt gacaagaatt ttgtcacacc tcccttattt attttgaaaa
     6661 tgagaatact a
//
LOCUS       DACDEA010000109         6670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDEA010000109 DACDEA010000000
VERSION     DACDEA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.111"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..474)
                     /locus_tag="IV136_002543"
     CDS             complement(16..474)
                     /locus_tag="IV136_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8466484.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(487..1812)
                     /locus_tag="IV136_002544"
     CDS             complement(487..1812)
                     /locus_tag="IV136_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8466485.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1829..3241)
                     /locus_tag="IV136_002545"
     CDS             complement(1829..3241)
                     /locus_tag="IV136_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466486.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3258..4022)
                     /locus_tag="IV136_002546"
     CDS             complement(3258..4022)
                     /locus_tag="IV136_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8466487.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4126..5001)
                     /locus_tag="IV136_002547"
     CDS             complement(4126..5001)
                     /locus_tag="IV136_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466488.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5256..5340)
                     /locus_tag="IV136_002548"
     tRNA            complement(5256..5340)
                     /locus_tag="IV136_002548"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5304..5306),aa:Leu,seq:caa)
     gene            complement(5358..5429)
                     /locus_tag="IV136_002549"
     tRNA            complement(5358..5429)
                     /locus_tag="IV136_002549"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Gln,seq:ttg)
     gene            complement(5439..5511)
                     /locus_tag="IV136_002550"
     tRNA            complement(5439..5511)
                     /locus_tag="IV136_002550"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5476..5478),aa:His,seq:gtg)
     gene            complement(5573..5657)
                     /locus_tag="IV136_002551"
     tRNA            complement(5573..5657)
                     /locus_tag="IV136_002551"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5621..5623),aa:Leu,seq:caa)
     gene            complement(5678..5748)
                     /locus_tag="IV136_002552"
     tRNA            complement(5678..5748)
                     /locus_tag="IV136_002552"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5714..5716),aa:Cys,seq:gca)
     gene            complement(5794..5865)
                     /locus_tag="IV136_002553"
     tRNA            complement(5794..5865)
                     /locus_tag="IV136_002553"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5831..5833),aa:Gln,seq:ttg)
     gene            complement(5877..5949)
                     /locus_tag="IV136_002554"
     tRNA            complement(5877..5949)
                     /locus_tag="IV136_002554"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5914..5916),aa:His,seq:gtg)
     gene            complement(5952..6025)
                     /locus_tag="IV136_002555"
     tRNA            complement(5952..6025)
                     /locus_tag="IV136_002555"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5991..5993),aa:Trp,seq:cca)
     gene            complement(6032..6112)
                     /locus_tag="IV136_002556"
     tRNA            complement(6032..6112)
                     /locus_tag="IV136_002556"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6076..6078),aa:Tyr,seq:gta)
     gene            complement(6114..6189)
                     /locus_tag="IV136_002557"
     tRNA            complement(6114..6189)
                     /locus_tag="IV136_002557"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6154..6156),aa:Phe,seq:gaa)
     gene            complement(6225..6297)
                     /locus_tag="IV136_002558"
     tRNA            complement(6225..6297)
                     /locus_tag="IV136_002558"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6262..6264),aa:Asp,seq:gtc)
     gene            complement(6307..6379)
                     /locus_tag="IV136_002559"
     tRNA            complement(6307..6379)
                     /locus_tag="IV136_002559"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6344..6346),aa:Val,seq:tac)
     gene            complement(6395..6466)
                     /locus_tag="IV136_002560"
     tRNA            complement(6395..6466)
                     /locus_tag="IV136_002560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6431..6433),aa:Glu,seq:ttc)
     gene            complement(6481..6570)
                     /locus_tag="IV136_002561"
     tRNA            complement(6481..6570)
                     /locus_tag="IV136_002561"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6534..6536),aa:Ser,seq:gga)
BASE COUNT         1998 a         1479 c         1094 g         2099 t
ORIGIN      
        1 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
       61 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      121 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      181 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      241 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      301 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      361 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      421 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      481 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      541 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      601 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      661 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      721 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      781 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      841 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
      901 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
      961 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1021 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1081 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1141 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1201 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1261 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1321 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1381 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1441 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1501 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1561 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1621 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1681 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1741 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1801 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1861 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     1921 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     1981 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2041 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2101 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2161 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2221 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2281 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2341 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2401 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2461 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2521 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2581 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2641 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2701 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2761 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2821 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     2881 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     2941 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3001 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3061 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3121 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3181 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3241 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3301 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3361 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3421 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3481 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3541 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3601 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3661 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3721 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3781 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3841 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     3901 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     3961 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4021 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4081 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4141 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4201 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4261 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4321 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4381 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4441 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4501 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4561 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4621 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4681 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4741 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4801 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4861 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     4921 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     4981 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5041 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5101 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5161 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5221 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5281 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5341 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5401 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
     5461 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
     5521 agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
     5581 gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc
     5641 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
     5701 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
     5761 tcaatctata aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc
     5821 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
     5881 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
     5941 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
     6001 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
     6061 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
     6121 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
     6181 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
     6241 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
     6301 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
     6361 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
     6421 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
     6481 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
     6541 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
     6601 aaatgagaac tccggcagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct
     6661 actaccaact
//
LOCUS       DACDEA010000110         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDEA010000110 DACDEA010000000
VERSION     DACDEA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.112"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV136_002562"
     CDS             725..811
                     /locus_tag="IV136_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8466489.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV136_002563"
     CDS             complement(932..1621)
                     /locus_tag="IV136_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466490.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV136_002564"
     CDS             complement(1628..1906)
                     /locus_tag="IV136_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466491.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV136_002565"
     CDS             complement(1866..2780)
                     /locus_tag="IV136_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8466492.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV136_002566"
     CDS             complement(2762..3142)
                     /locus_tag="IV136_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8466493.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV136_002567"
     CDS             complement(3592..3879)
                     /locus_tag="IV136_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8466494.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV136_002568"
     CDS             complement(3899..4123)
                     /locus_tag="IV136_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8466495.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV136_002569"
     CDS             complement(4763..5296)
                     /locus_tag="IV136_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8466496.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV136_002570"
     CDS             complement(5317..6159)
                     /locus_tag="IV136_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8466497.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDEA010000111         6101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDEA010000111 DACDEA010000000
VERSION     DACDEA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.113"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(312..797)
                     /locus_tag="IV136_002571"
     CDS             complement(312..797)
                     /locus_tag="IV136_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466498.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="IV136_002572"
     CDS             complement(936..1151)
                     /locus_tag="IV136_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8466499.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1375..2175
                     /locus_tag="IV136_002573"
     CDS             1375..2175
                     /locus_tag="IV136_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8466500.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2194..4062
                     /locus_tag="IV136_002574"
     CDS             2194..4062
                     /locus_tag="IV136_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8466501.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4055..4546
                     /locus_tag="IV136_002575"
     CDS             4055..4546
                     /locus_tag="IV136_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8466502.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4534..5088
                     /gene="srlA"
                     /locus_tag="IV136_002576"
     CDS             4534..5088
                     /gene="srlA"
                     /locus_tag="IV136_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8466503.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5106..6101
                     /locus_tag="IV136_002577"
     CDS             5106..6101
                     /locus_tag="IV136_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8466504.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2157 a          968 c         1222 g         1754 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     1201 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     1261 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     1321 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     1381 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     1441 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     1501 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     1561 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     1621 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     1681 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     1741 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     1801 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     1861 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     1921 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     1981 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     2041 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     2101 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     2161 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     2221 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     2281 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     2341 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     2401 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     2461 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     2521 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     2581 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     2641 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     2701 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     2761 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     2821 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     2881 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     2941 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     3001 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     3061 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     3121 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     3181 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     3241 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     3301 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     3361 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     3421 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     3481 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     3541 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     3601 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     3661 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     3721 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     3781 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     3841 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     3901 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     3961 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     4021 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     4081 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     4141 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     4201 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     4261 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     4321 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     4381 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     4441 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     4501 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     4561 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     4621 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     4681 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     4741 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     4801 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     4861 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     4921 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     4981 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     5041 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     5101 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     5161 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     5221 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     5281 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     5341 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     5401 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     5461 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     5521 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     5581 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     5641 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     5701 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     5761 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     5821 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     5881 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     5941 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     6001 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     6061 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DACDEA010000112         5863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDEA010000112 DACDEA010000000
VERSION     DACDEA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.114"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV136_002578"
     CDS             complement(99..1229)
                     /locus_tag="IV136_002578"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8466505.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV136_002579"
     CDS             complement(1878..3080)
                     /locus_tag="IV136_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466506.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV136_002580"
     CDS             complement(3140..3343)
                     /locus_tag="IV136_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8466507.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV136_002581"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV136_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV136_002582"
     CDS             4880..5692
                     /locus_tag="IV136_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466508.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1210 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttc
//
LOCUS       DACDEA010000113         5841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDEA010000113 DACDEA010000000
VERSION     DACDEA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.115"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..3161)
                     /locus_tag="IV136_002583"
     CDS             complement(12..3161)
                     /locus_tag="IV136_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8466509.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3175..4392)
                     /locus_tag="IV136_002584"
     CDS             complement(3175..4392)
                     /locus_tag="IV136_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8466510.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4382..>5841)
                     /locus_tag="IV136_002585"
     CDS             complement(4382..>5841)
                     /locus_tag="IV136_002585"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8466511.1"
                     /translation="VLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGY
                     SISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQ
                     QRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMM
                     AQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMN
                     LILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNR
                     YGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYA
                     VIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILE
                     TYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQ
                     QVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1582 a         1245 c          984 g         2030 t
ORIGIN      
        1 actcttctcg tttacctcaa ttcattttca agtggaatca actcatcatc taacatttta
       61 cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct
      121 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
      181 cctggcatat attgcagatt agaagcatgt aattctttct cagaaacaaa ccactcattg
      241 gaaaaggcat gaatcaaatt atcacttgct tgtgtaaaat agcgacgttt aatgacaaag
      301 atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg tcggtaaact
      361 tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc ttctcgtgca
      421 tctgaactat tcgcagcata agttcctagt aattgatcaa tataagtaga atcaatatca
      481 tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc tccctctttt
      541 tctggttctt cctccatcaa tgaacccttc acggtctcgt acacgccaat ttgttcttcc
      601 aaaagttgaa tttgattgtt caaagttgaa gattgcacca tgtcatcatt gtaatcatca
      661 taagtcacta acgattcata cgcacgattc aattcttgaa atgctttgac gtactcgata
      721 cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc ttttaactta
      781 ccatatgctt gcttgaatct ttttttggat tcttggtaac ttggataaat caaacccgat
      841 tcttctttag cttctgaata cagttttgtc gcatcctcga cattcttctc cattgtcgct
      901 ggctttcgga aagtaacaat tagtcctttt gttttttcag gatacgtacg gttcgtacgt
      961 gaaaaggctt ggatcaatcc tgcatactct agattacgat ccacaaatag ggtttgaatc
     1021 gtaggggcgt caaaacctgt caatagacga tccactacga ttactaaatc gatttgtttc
     1081 ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc tccgttataa
     1141 cgctcaatgt ctgctagtga ccaggcagta tcataatact gattatattc ctcaataatt
     1201 ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa tgaataggta
     1261 atagcgattc gtgggaaatc aggatcctcc atcgttcgtc cttcacggat aggctgattg
     1321 ggaaactctt ttattaacca atcagattct ttcgtcatct ctttgatggc ccgataatac
     1381 tttttggcca tatcaatgga actcgtggtt aagatcgcag acttcgttgg acggccattt
     1441 cgaaaatcaa attttgtata ggcattgtct ggacggaaca ttttatgaat gactttttgg
     1501 atatgttcct ctgcttcaaa aacagtaggg tccaaataac tttctttttt gacagactcc
     1561 atttgatcga tcatacgatt gatctgttcc ggtgaatact ctgcatatgt ttctacttct
     1621 tttaatttcc gatagatttt attctctaat gaagtcggct ccacggtgtt ttcatgctct
     1681 acttgaaatc ctaaaaccga gccatcttct aatgcattct tgatggtata ggtgtgcaag
     1741 acctctccat actgatcatg ggtcgtacgt gccaattgcc ctttggcttg tttccgattc
     1801 tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa catttttttg
     1861 atttcttcca tattttcagc acttaacgct cgatgacact catcaactac aaacacgata
     1921 tgttgcccca ttaatttttg gaagcgattg gttccttttt tctcttcctg tttctttgca
     1981 tatttcaacg ccgcatctag tttttgacgg gttgtaatga tcacaacatt cgcattggca
     2041 tctgccaaca gagtttcact taactctttg gcacttcctg ttcccacgat caagctattc
     2101 gctttggcat taccagaaga aaccccagta ttaaactccg aagcaaattt cgtaaactct
     2161 gtggtggtct gattatctaa atctttgcgg tccactaaca tgatcgttcg atcgatcccc
     2221 gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc tgaaccagtc
     2281 gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca
     2341 tgcacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc actgacaatc
     2401 gtataatccg caatcaagcg atgggcatct gggatattta gcacttgttt gcaaaactca
     2461 tataagtttt ctaccttttt attatctttc gttcgccaac taaataaaaa cttaggatgc
     2521 aaatccttcg gtaaggcatt tgcaaaatac cgagtcgttt gttcattgga aaccacaaac
     2581 aactgtaagg tcgaaaagat attgttccga aacatgcctt cttcagcata ttttttgatt
     2641 tgattaaacg cctgataaaa accatctttt gcagttactt gttttagctc gatttggata
     2701 atcggtaagc cattgatcaa taaggtgacg tcaaaacgtc gatcccgcgc ttctatgtta
     2761 cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc accaatgtct
     2821 tgattggaat aaaggataag tgagacagaa cccaattgcg gattttcccg ttcaatcgtg
     2881 atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg ggttttcgtc
     2941 cgtagcaata attcggtttt gatcgtttca aactctttat cggtcaaagg agaatcattc
     3001 agttctgctt gattatttga gatgattttt tgacgtaaat tgctccaaag atcttcttct
     3061 gattttagat ccggacgata cgtccactga ttatgtcctt ccccgaggac acgaatcaac
     3121 tgttcttcga cttccgcttc ctcacggtga ctaattttca ttcttcttcc ctccctaaac
     3181 aaacatcttt tgcaagaagc cttttttcgt ttccttcagc aaatctaact tacgttgatg
     3241 aagagcgatc gtatcatcta gctgcttgaa gaaggcacca attttttgtt gttcatttat
     3301 atccggtgga aactgaaatg tcctttgttc tataccacct ttagtcacat gtaccattgt
     3361 cgttccgtta gttgattctt taattttctc tttatcagtc aatagccaag tatatagata
     3421 ctgtttgtcc aattccttag ataaaaattt aagtttccaa atatgataat ggtagattac
     3481 acgttcttca ttccacattt caggaccgaa gcttgttgcc cataaatata agagatctcc
     3541 tttattagca tacttgtctt cctctaattc taagtttgaa taataccatc tatcattagt
     3601 attaaaatta cctactctta aaaccctata ttttccagaa tcaagtaatt cttgttgttt
     3661 aaaagctcta ccattcaaaa aaactaccac ttcccccaac ttccgctctt cccaatctcc
     3721 cgtaaatcct ggaaaacgaa cttctggcac ttttgctccg tttttaggga acattttttg
     3781 taagaatcct tttttcgttt ccttcagcaa atctaattcg tgctggtgaa gcatgatggt
     3841 gtgatcaagt tgtttgaaaa atgaaccgat cttgatctgt tcgtcataag ttggaagtga
     3901 gataggtgtc tttttaccac tatcaattgt ataggttccg acggttccaa tgcctgcaac
     3961 gtcacggata tacttctgaa ttttgttatt tatgaacgag tagtaaaaga agtttccgtc
     4021 aattttatgc tcattcttga accaaatgat gtttccatcc ttaaagtaaa ttgggttatc
     4081 atcagtgatt aataagggga cacctattga tccgacacca gttacaagca agtctccttg
     4141 agaaacttta cctgaatttt tcgaatattc attatatttt tcttctgaaa tgtaaagata
     4201 atcggatggt tcctcatttt ttgcagctga gacaatatca cgagcacgta aaaatctaat
     4261 acctgagtta gtccaatctg attgatgaat tcgcttcaca gaagtaacat ccatcatatc
     4321 tcctaactta cgctcttccc aatcttccgt aaaaccagga aaccggattt ctggtcgtgt
     4381 atcattgctc atgtttgaac acctccagcg caccttgtaa ccacgcttca ttttcaggtg
     4441 ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc tgttcttgtc
     4501 gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct tcttcgaaag
     4561 tatcgacata gcgtgggata tttaagttat agtcattttc tttgatttca tcaaaggttg
     4621 ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg attttttgaa
     4681 tgttttcttc cgataattta ttctgatttt tccctttaac aaattcacga ctagcatcaa
     4741 tgaataatac atctcgagtt tgacgattct tcttcaatac aatcaccgtc gttggaatcg
     4801 atgtaccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg ccgtcttcta
     4861 ataatttttg acgaatgacc ccttctgcag caccacggaa taaaaccccg tgtggcaaga
     4921 cgatagccat agtccctgtt tccttcaaat gatagaaacc atgaagaaga aacgcaaaat
     4981 ctgctttgga ttttggcgct aatttcccgt agcgattaaa ccgagaatca tctaagaagg
     5041 tcgtatctgc tgaccaatta gccgaataag gtggattcat cactactgca tcaaatgtgt
     5101 aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga ttcatttctt
     5161 cggcatctac cccatgtaaa atcaagttca tttttgctag attgtatgtc gttgtattta
     5221 gttcttgccc atgatattta acgttatctg gatgagttaa gtagttacgt acatttaaca
     5281 tcaaggaacc tgaacccatg gttgggtcaa agacactaaa gaatcgtcgt tctttttgat
     5341 ccaacgtcac gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa aattctccgg
     5401 ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ttcatacgca tccccgatca
     5461 catccccatc gtgacctaaa acttccacgt cattcaattt cttgataact tcggtaatcg
     5521 tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc acatcatcaa
     5581 ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg tttagatcgt
     5641 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggtg
     5701 agatcgaata ccccaaaata tcaacgattg tggcaatcag gtcttctttt gattcttcat
     5761 ctgaaagtaa ttcacgatac aacattgttt gtttgcttac tgtgtcatat tcctctaagg
     5821 attcatctgc taaaagaacc a
//
LOCUS       DACDEA010000114         5808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDEA010000114 DACDEA010000000
VERSION     DACDEA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.116"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(362..1069)
                     /locus_tag="IV136_002586"
     CDS             complement(362..1069)
                     /locus_tag="IV136_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466512.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1134..1970)
                     /locus_tag="IV136_002587"
     CDS             complement(1134..1970)
                     /locus_tag="IV136_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466513.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1988..3397)
                     /locus_tag="IV136_002588"
     CDS             complement(1988..3397)
                     /locus_tag="IV136_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8466514.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3411..5234)
                     /locus_tag="IV136_002589"
     CDS             complement(3411..5234)
                     /locus_tag="IV136_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466515.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1784 a         1260 c          949 g         1815 t
ORIGIN      
        1 ttacatatga ttttttcata aaaaaacagt actgtctagt agtataaaat atacaaattt
       61 cttaaaaatt ttttcactaa aatcactacc tgaaacagtc taaaacactt atatatcaac
      121 gtttaatgaa actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat
      181 ccgaaaaaaa atacccccag tagcttatcc taagctactg gagatgtata agtacggtca
      241 tataacaacg gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt
      301 ttttaaatac ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt
      361 tttacccatt ttcaagtaac cgaagtggaa tgtctttacc aacttgctct gtcactgcat
      421 tactgatacc tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct
      481 ctgtatcagt atttatcgtg attcctttat cttcatttac aagcttccaa gtaggtggta
      541 tagctgtgta tgtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta
      601 cttctgtctc attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg
      661 cttctgtctc attattctca ccggaaactc tcttcacacc caaacttata tattgtccat
      721 ctttagtgtg catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc
      781 tttcaataga gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta
      841 aaagcatttt gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta
      901 tacggaatac agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc
      961 aatctccaag tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac
     1021 tggtagaggt cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag
     1081 ccttagcaca accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac
     1141 acccttgaaa tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc
     1201 tcttcttgaa cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc
     1261 ttcactgaag ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg
     1321 gcaataaaaa actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc
     1381 tttttttcgg gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag
     1441 agcatggttt cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg
     1501 tttccagcct cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca
     1561 tagcagccga tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt
     1621 tgaaaaacaa tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta
     1681 aaaaatagac ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc
     1741 ttgacgcaaa taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt
     1801 agagcatcag aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa
     1861 tcgcctttct ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc
     1921 ttatctaagc aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc
     1981 gttctctcta agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt
     2041 tttttattcg ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat
     2101 aacgcttatt gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac
     2161 caaaaacatc tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat
     2221 aatcaattcg atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat
     2281 agccattttc agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta
     2341 atcgtagtcc agtagggtca actacccagc catatggcgt tttatctaca taaggatttt
     2401 cagctgcccg gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg
     2461 aaacatctga tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg
     2521 caataaaatc gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat
     2581 ataatccacg atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta
     2641 catctaagaa agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg
     2701 gactggaggt agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa
     2761 gattcaactg atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa
     2821 tttgaaagag ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag
     2881 gatgaaggat catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat
     2941 actcagtaag caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc
     3001 aaccgccctg ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt
     3061 cgattccctg attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat
     3121 tggttttatt attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt
     3181 ttaagcctaa ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg
     3241 caacttcaaa attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt
     3301 tcccatcctc ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa
     3361 acttctctgg aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt
     3421 tgcatcaatg gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt
     3481 tcactgttgg tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg
     3541 aaatcggcgt tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact
     3601 tcaaagggtt ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata
     3661 ccttctttag aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc
     3721 tcaacaggtg aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt
     3781 cccttagaaa agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga
     3841 ttttcaacaa tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca
     3901 aatccaatta gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt
     3961 gcgtgaacaa tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg
     4021 gaaacaaagg catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca
     4081 ccaatcatgg aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc
     4141 tcagtaattc ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg
     4201 tttttagttt tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc
     4261 attcctggcc cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc
     4321 gtcattgccc aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat
     4381 ggaattaacc aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc
     4441 agcaacgttc caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc
     4501 agcatcggct tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct
     4561 acataggaca tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca
     4621 gctaataccg gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata
     4681 tttggatgaa gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt
     4741 atggctgccc cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta
     4801 ttcaaaatga tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc
     4861 aaaatggctt ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta
     4921 gaaatgacac taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt
     4981 ttaattgaag ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca
     5041 atcaccactt ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct
     5101 aaggctttcg tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa
     5161 tgggttaagc tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact
     5221 tctctgtaat ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc
     5281 cccaatcaac attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc
     5341 aataacgtta ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga
     5401 atttaatttt ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca
     5461 actagggtat atctgcgtat acttactgct aaaataagta tatctaatat taactatacc
     5521 agtaaaacca ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat
     5581 gcatataacg gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg
     5641 tgagtaaagg atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt
     5701 tttttggtaa aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc
     5761 gatagaatga tttgggctag tattcttaaa aattaactag caaatgtt
//
LOCUS       DACDEA010000115         5595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDEA010000115 DACDEA010000000
VERSION     DACDEA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.117"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..485
                     /locus_tag="IV136_002590"
     CDS             <1..485
                     /locus_tag="IV136_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8466516.1"
                     /translation="DASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKLENS
                     NLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNGIDR
                     ENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFTNYR
                     "
     gene            complement(509..2884)
                     /locus_tag="IV136_002591"
     CDS             complement(509..2884)
                     /locus_tag="IV136_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8466517.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3210..3836
                     /locus_tag="IV136_002592"
     CDS             3210..3836
                     /locus_tag="IV136_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8466518.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            3859..4563
                     /locus_tag="IV136_002593"
     CDS             3859..4563
                     /locus_tag="IV136_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8466519.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            4755..5588
                     /locus_tag="IV136_002594"
     CDS             4755..5588
                     /locus_tag="IV136_002594"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8466520.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         1667 a         1081 c         1043 g         1804 t
ORIGIN      
        1 aagatgctag cttcgacatt gtatcagaag tcaatacaga agaagtaaaa aatgcgattc
       61 agcaaacaac aaaagaattg tccaatcgct ttgacttcaa ggattctaca gtagaaatca
      121 aattagagaa cagcaatcta gttgtattga gcgatgatga tttcaaaatc gaacagatca
      181 aagatgtatt atttggtaag ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt
      241 ccgaatcaga acacgctcta ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa
      301 tcgatcgtga aaacgcgaag aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa
      361 aagcacaaat ccaagaagat cagattagag tgacaggtaa gaaccgtgat gatctacaag
      421 ctgtgattgc tttattacgt aaacttgact taccaatcga gttgcaattc actaactatc
      481 gttaatcaaa aaacgaggct gacgtctgtc attcctcgtt tttttgattt actgcttctt
      541 ttgataacca aaaacgctgt attccttctt ccaattcccc aacacttcgt acttctcttg
      601 ctgtttgcca atgttgtggg aataaacgtc gataagaaaa actagaaaat cgttgatctg
      661 ccaatacaac gattccttga tccgccatgc cacgaatcac tcgaccacca gcttgcaaga
      721 ctttgttcat tccaggaagc tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat
      781 aatatgattt gatcagttcc tgctcatgat tcatctgcgg caaacctaca cctacgatca
      841 tactgccgat caatcggtcc cctctcaaat cgattccttc tgaaaaaatc ccgccaagta
      901 cgcaaaatcc aatcaatgtt tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc
      961 gttcttcctc attcatgtcc gtttcttgga ttagcacatt gctgtcggga tattgctgtc
     1021 tgaatgcttc caccgcaaga tctaaatatt gataagaagg aaagaaaacg aaataattcc
     1081 cgacctttcc atcagcaaaa gcatgaatcg tctctactaa ttttgccaga ctgtgttccc
     1141 tttttcgata agtcgtctct aggtaatttg cgatgaaaac ttgctggttt ccttctggga
     1201 aaggactcgg cagtttgtaa gccagacttt tttgtccccc cagtgtttcc tgataataag
     1261 agagtggtga aaaactagca gaaaaaagaa tactgctttt tcctttatcc aacgactgct
     1321 caagaaacaa gcttggatca atacaaaact cttttactat cagatcgcga tacgtttttt
     1381 cgactgttgt ttcgtagtga tcatcataaa attcactgac cttcaaaaaa tgaagcaaat
     1441 caaaataata aggaagcaat tgttcttgtg atgggtgctc aggaaattct gccagccatt
     1501 ctttaatttt ttcgcttaat tgataacctg cctttactaa agattcagcc ggtgcttttt
     1561 gatgatgata atcccaatga tcttcttttg ctatttctcg gatcgaatca aattctttta
     1621 atagtttgtt gaaacggcga tgaagttttt tgaattcctt tggtatagct tccttacttc
     1681 gtttcgtttt ttcataagat agttctgccg aatacatctc tcgggaacga ttcaccaaat
     1741 tatgtgcttc gtcaatcaaa aacagatagt cctcgttttt ttcatcttca aaaaaacgtc
     1801 ttaaataaac agttggatca aataaataat tgtaatcccc aatgattacg tcacaccaga
     1861 tactgacatc taatgatagt tcgaaaggac aaagtgtatg ctttcttgca taagtttcga
     1921 tgacttcacg agtgatctga ttctcatgat tcaaaagatc ccataaacct tcattaatcc
     1981 gattgtaata accattggca tatgggcact gatctggatt acaggtcgtc tcatctaaaa
     2041 agcagatctt gtctttcgcc gtcaaagtga cactttttac ttcagctcct gtatctttta
     2101 atgctgtcat agcatcttca gctacttgtc tagttattgt tttagccgtt agataaaaga
     2161 tcttttctcc ctcttcttcc cctacagctt tcaaagcggg gaataacgta gagattgttt
     2221 ttcctgttcc ggtaggtgct tctacaaaca atttctgttt cgtacggatc gttttataag
     2281 cagctaccgc tagttcacgc tgacctttcc tatactcatc atgggggaat ttcaaagcca
     2341 tcaaagaggc attgcgtaca gtgcgccagt tttcttgaaa aacaagccat tctttataat
     2401 cttcagtcag ctttttaaaa aaagtttcta actgctctct tgtctgatgt tcaacctttc
     2461 tcgtgatcac ttcttctgcc gtctggaagt aagtcaattg cagatttatt tcttgtaaat
     2521 catgttcttg actatagata taggcataca cccttgcttg atgaaagaaa agttccactt
     2581 gttctggttc taaatcttca aatcgtggtt cggaggtttt gatttcatct atataataaa
     2641 taccgtctct tttgaaaata ccgtctgcac ggccttcaac gatcaggtaa tattcatcta
     2701 attgaactgt tgtttttaaa aagacttctt tttcatagtt ttctcccgca tctttttgga
     2761 gcttgcggtg gattctcgct ccttctaatg cagtatggtt acttttttta cgattgtcga
     2821 tacttccacg tcgcaaaata aattctacta agtgccgcac agatattttt gactgtttga
     2881 tcatttccac cacctctttc tctctaagat tacctgaaaa aacctagttt agcaaaaaga
     2941 gctctctttc caaacataaa aaaacaccct gctgattagg cagggatata ggatatagaa
     3001 tttggatttt tggatgaatc taatgtaaca aggacatatt aaaatgctat gaaaacttac
     3061 tttccttctc tttagaatat ccaaatactt ttcatatagt ctgataattc agataagtaa
     3121 agagaaataa gtacctgttt ttgtttccta gcaatgaatg cgttatacta atggatgaaa
     3181 gaagttttgg ctatcaagga ggaatgcaca tgcatatcga aagattaaaa acaggaatga
     3241 ttgaagaaaa ctgttatttg gtctacaacg aagaagcact tttgatcatt gaccctggag
     3301 aagatgcgga acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg gtagcgattt
     3361 tgcttaccca tactcactac gatcatatcg gtgcagtgga acctttacgc gcgtattatc
     3421 aaatcccagt ttatgtcagc ccactagagc aaaaatggct aggtgatccg attttgaatc
     3481 tttctggact cggtagacat gatgatattg ctgatgttat tgtctcgcct gcagaatatg
     3541 aatttgaaat gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc cctaccccag
     3601 ggcattctat aggaagcgtc agctttattt ttgatgattt tgtcgtttct ggcgacgccc
     3661 tgtttagagg aagtatcggt agaaccgatc tgtatacagg gaatttagaa caattacttc
     3721 atagtattcg gacccagctg tttgtattac ctgatgaatt tgcagtctat ccaggacatg
     3781 gcgatgcaac gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg
     3841 acttaggagg agaataaaat gacagaaact acactttata tcgtacgaca cggaaaaacg
     3901 atgttcaata caattgaacg agtacaaggc tggtgtgata cacccctcac aaagcaagga
     3961 caagaaggca ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat
     4021 tctagtgaca gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga acattctaag
     4081 ggaaaagaaa ttccttattt tattgaaaaa aggattagag aatggtgttt cgggtctctt
     4141 gaaggtggat atgacatgga aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca
     4201 tatgatgaaa tgtttaccac agaagtttct tttgaacaaa ttgctaatgc tgtatacaac
     4261 ttagataccg ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt atggacagga
     4321 tttgaagata tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct
     4381 catggtttga cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct
     4441 gggcttttaa atggcagcgt aacaaaggtc acttatgcaa acggtcgatt ttctatccac
     4501 ggcatcaatg atacgtctta tatcgataac gggaaaacac atagtcgcga ctcgtttcta
     4561 taatggggat attttaattt ttaccagaga atcataaaag gcaaagtctg aacgagggct
     4621 ttgtcttctt ttttctcttc gtgtacttat ctgctttatt tctcaagttt ttcttaatat
     4681 taaaaaacag attgggtttc tcaaaaaata acggtatact ataagatgta tcttaattgg
     4741 gaaggaagga atttatgttt tttgcaaata ttatcaaagc agtgattctc ggtattattg
     4801 aaggaataac ggaatggctg cccatcagta gtactggtca tttgatccta gcagatgaat
     4861 tcatcaaact ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt atccaattag
     4921 gggcgatctt ggctgtcgtc gttctttatt ttcacaagtt aaacccattt tctccaagta
     4981 aatcaacggt agaaaaaaaa gatacgtgga cactttggtt taaagtgatc gttgcttgca
     5041 taccagccat catcattggt ttgcctttgg atgattggtt agaagcaaat ttccacaagt
     5101 tcttaccagt tgccattgtt ttgatcgttt acggtattgc ctttattatt gtcgaaaaac
     5161 gcaacaaaaa caaaacacct cgttggactt ctctgaatga ctttacgtac caagcagctc
     5221 ttcttgttgg gttattccaa gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa
     5281 cgatcctagg atctatcttg attggtgctt cgcgttttgt cgctacagag ttttcatttt
     5341 tcttagggat tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg
     5401 aagggaattc attcaaaatg gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt
     5461 ttgtggtttc cgtcttagct atcaaattct tactaagcta tttgaagaaa aacgacttta
     5521 ccgcttttgg ctggtatcgt attatccttg gtatcatttt gattggttat tggtttattg
     5581 caatgtagaa aaaac
//
LOCUS       DACDEA010000116         5109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDEA010000116 DACDEA010000000
VERSION     DACDEA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.118"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IV136_002595"
     CDS             join(107..335,335..1248)
                     /locus_tag="IV136_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8466521.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="IV136_002596"
     CDS             1483..1935
                     /locus_tag="IV136_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466522.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="IV136_002597"
     CDS             1949..2638
                     /locus_tag="IV136_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466523.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..>5109
                     /locus_tag="IV136_002598"
     CDS             2763..>5109
                     /locus_tag="IV136_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8466524.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKL"
BASE COUNT         1738 a          780 c         1045 g         1546 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaactta
//
LOCUS       DACDEA010000117         5023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDEA010000117 DACDEA010000000
VERSION     DACDEA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.119"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IV136_002599"
     CDS             complement(406..780)
                     /locus_tag="IV136_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8466525.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IV136_002600"
     CDS             complement(803..2203)
                     /locus_tag="IV136_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8466526.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IV136_002601"
     CDS             complement(2207..2617)
                     /locus_tag="IV136_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8466527.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IV136_002602"
     CDS             complement(2635..3126)
                     /locus_tag="IV136_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8466528.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IV136_002603"
     CDS             complement(3136..3927)
                     /locus_tag="IV136_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8466529.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IV136_002604"
     CDS             complement(3920..4693)
                     /locus_tag="IV136_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8466530.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5023)
                     /locus_tag="IV136_002605"
     CDS             complement(4849..>5023)
                     /locus_tag="IV136_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8466531.1"
                     /translation="MIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGE
                     YELIYTIFEQTIF"
BASE COUNT         1598 a         1022 c          661 g         1742 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaggtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ata
//
LOCUS       DACDEA010000118         5004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDEA010000118 DACDEA010000000
VERSION     DACDEA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.120"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IV136_002606"
     CDS             <1..104
                     /locus_tag="IV136_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAP8466532.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="IV136_002607"
                     /pseudo
     CDS             792..1613
                     /locus_tag="IV136_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1756..1980
                     /locus_tag="IV136_002608"
     CDS             1756..1980
                     /locus_tag="IV136_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466533.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="IV136_002609"
     CDS             1995..2864
                     /locus_tag="IV136_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8466534.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="IV136_002610"
     CDS             2845..3579
                     /locus_tag="IV136_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8466535.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3612..4520
                     /locus_tag="IV136_002611"
     CDS             3612..4520
                     /locus_tag="IV136_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8466536.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4517..4765
                     /locus_tag="IV136_002612"
     CDS             4517..4765
                     /locus_tag="IV136_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP8466537.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(4768..>5004)
                     /locus_tag="IV136_002613"
     CDS             complement(4768..>5004)
                     /locus_tag="IV136_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8466538.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1690 a          757 c         1084 g         1473 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3661 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3721 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     3781 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     3841 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     3901 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     3961 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4021 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4081 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4141 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4201 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4261 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4321 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     4381 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     4441 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     4501 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     4561 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4621 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4681 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4741 tcttttaacc cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttcctcc
     4801 aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct
     4861 actctctagc cctttaacag aacgtgagtt gaaattttga caacctaatt tttctaagga
     4921 agaaaaaaca gtctcaactg tttttctttg ttttccaagt aagctgtggt ctagtttttc
     4981 tgatttatcc atgtttttac gtgg
//
LOCUS       DACDEA010000119         4953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDEA010000119 DACDEA010000000
VERSION     DACDEA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.121"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(639..1142)
                     /locus_tag="IV136_002614"
     CDS             complement(639..1142)
                     /locus_tag="IV136_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8466539.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1501..2526
                     /locus_tag="IV136_002615"
     CDS             1501..2526
                     /locus_tag="IV136_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8466540.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2849..3727
                     /locus_tag="IV136_002616"
     CDS             2849..3727
                     /locus_tag="IV136_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466541.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4203..4319
                     /locus_tag="IV136_002617"
     CDS             4203..4319
                     /locus_tag="IV136_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8466542.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1664 a          781 c          890 g         1618 t
ORIGIN      
        1 aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac agaaatgaat
       61 aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata aaaaaataac
      121 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
      181 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
      241 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
      301 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
      361 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
      421 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
      481 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
      541 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
      601 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
      661 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
      721 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
      781 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
      841 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
      901 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
      961 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
     1021 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
     1081 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
     1141 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
     1201 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
     1261 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
     1321 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
     1381 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
     1441 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
     1501 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
     1561 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
     1621 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
     1681 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
     1741 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
     1801 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
     1861 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
     1921 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
     1981 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
     2041 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
     2101 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
     2161 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
     2221 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
     2281 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
     2341 aatatttttc ttaatctgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
     2401 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
     2461 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
     2521 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
     2581 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
     2641 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
     2701 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
     2761 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
     2821 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
     2881 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
     2941 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
     3001 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
     3061 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
     3121 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
     3181 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
     3241 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
     3301 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
     3361 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
     3421 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
     3481 tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa aaatctgtct
     3541 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
     3601 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
     3661 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
     3721 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
     3781 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
     3841 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     3901 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
     3961 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
     4021 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
     4081 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
     4141 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
     4201 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
     4261 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
     4321 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
     4381 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
     4441 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
     4501 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
     4561 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
     4621 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
     4681 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
     4741 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
     4801 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
     4861 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
     4921 tcaaggaata ataagttttg gttttcagat aat
//
LOCUS       DACDEA010000120         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDEA010000120 DACDEA010000000
VERSION     DACDEA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.123"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..358
                     /locus_tag="IV136_002618"
                     /pseudo
     CDS             107..358
                     /locus_tag="IV136_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="IV136_002619"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="IV136_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8466543.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1766
                     /locus_tag="IV136_002620"
                     /pseudo
     CDS             1655..1766
                     /locus_tag="IV136_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="IV136_002621"
     CDS             1787..1870
                     /locus_tag="IV136_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8466544.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="IV136_002622"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="IV136_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8466545.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="IV136_002623"
     CDS             complement(2995..3858)
                     /locus_tag="IV136_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8466546.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="IV136_002624"
     CDS             complement(3860..4132)
                     /locus_tag="IV136_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8466547.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4364)
                     /locus_tag="IV136_002625"
     CDS             complement(4149..4364)
                     /locus_tag="IV136_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8466548.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4456..>4710)
                     /locus_tag="IV136_002626"
     CDS             complement(4456..>4710)
                     /locus_tag="IV136_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8466549.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          795 c          865 g         1605 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct caagcatttc
     4501 aaaaaatacg ttcttataca tggataaaac ttttttgtca tagcctttat gctctgtaat
     4561 gccgttttta gaccaggtac ttactttatt acttcgcttg ataatatttt ggaaaaccaa
     4621 gttatcttct ttatgttgct tatacagttc ttccaggttt gattttatcg ttgcgctgtc
     4681 cgtatcaact aaataaggaa caaaaccgat
//
LOCUS       DACDEA010000121         4686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDEA010000121 DACDEA010000000
VERSION     DACDEA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.124"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IV136_002627"
     CDS             complement(117..1079)
                     /locus_tag="IV136_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8466550.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IV136_002628"
     CDS             complement(1081..2520)
                     /locus_tag="IV136_002628"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8466551.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IV136_002629"
     CDS             2705..4651
                     /locus_tag="IV136_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8466552.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1481 a          803 c          774 g         1628 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttc
//
LOCUS       DACDEA010000122         4590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDEA010000122 DACDEA010000000
VERSION     DACDEA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.125"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            148..1137
                     /locus_tag="IV136_002630"
     CDS             148..1137
                     /locus_tag="IV136_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8466553.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IV136_002631"
     CDS             1134..1553
                     /locus_tag="IV136_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466554.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IV136_002632"
     CDS             1562..1870
                     /locus_tag="IV136_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466555.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IV136_002633"
     CDS             1874..2236
                     /locus_tag="IV136_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466556.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IV136_002634"
     CDS             2458..3864
                     /locus_tag="IV136_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8466557.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1598 a          651 c         1042 g         1299 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DACDEA010000123         4547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDEA010000123 DACDEA010000000
VERSION     DACDEA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.126"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(171..782)
                     /locus_tag="IV136_002635"
     CDS             complement(171..782)
                     /locus_tag="IV136_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8466558.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(905..1030)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1112..1828)
                     /locus_tag="IV136_002636"
     CDS             complement(1112..1828)
                     /locus_tag="IV136_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8466559.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1834..2403)
                     /gene="scpB"
                     /locus_tag="IV136_002637"
     CDS             complement(1834..2403)
                     /gene="scpB"
                     /locus_tag="IV136_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8466560.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2387..3184)
                     /locus_tag="IV136_002638"
     CDS             complement(2387..3184)
                     /locus_tag="IV136_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8466561.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3154..3525)
                     /locus_tag="IV136_002639"
     CDS             complement(3154..3525)
                     /locus_tag="IV136_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8466562.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3544..4431)
                     /gene="xerD"
                     /locus_tag="IV136_002640"
     CDS             complement(3544..4431)
                     /gene="xerD"
                     /locus_tag="IV136_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8466563.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1250 a          985 c          788 g         1524 t
ORIGIN      
        1 attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt gatttttatg
       61 cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt gcatgtcttg
      121 tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat ttatttgact
      181 gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag aagttgcgca
      241 tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa aggaacgatc
      301 ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt cagccctaaa
      361 aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa aatagttaga
      421 gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg tttctttaca
      481 ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact agcagtgtct
      541 cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga acgcaagaac
      601 gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac cgagatatca
      661 ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa
      721 actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg ggtgttcttc
      781 atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt ctctccaaat
      841 gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa
      901 taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac cgattcagct
      961 atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca ccctgcccct
     1021 gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta ttcaaaaaac
     1081 gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc aaactgctga
     1141 cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa gtcagatctc
     1201 cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc atatttttca
     1261 cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt gtcttaggat
     1321 ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc tttattcctt
     1381 ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca acgtaaactt
     1441 tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca tttgtcagta
     1501 gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt tcttttacca
     1561 tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca gaaataactc
     1621 ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg acttcatcca
     1681 cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact tgtccgttta
     1741 cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg atgccagcgt
     1801 gggcaattac tttctgcaat ctctccatta caattattct cctttattca ttccatcaaa
     1861 gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg tatcaggcag
     1921 ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc catataatat
     1981 cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg cagctagacg
     2041 ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc ttgtgattgg
     2101 ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg tgtttgatgt
     2161 cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta atacataatg
     2221 gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag attcttttaa
     2281 ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc ttaattcttc
     2341 attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac ttaatttcgt
     2401 catgttcggt atctcctact gctggataaa gcataattga ttcatagttt tctttttgtt
     2461 gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta gtgacaactt
     2521 cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca tttcttttta
     2581 tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt
     2641 tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca cggatcaaat
     2701 cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac ccatcgatgt
     2761 tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc agtctttcag
     2821 ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct cgaggatctt
     2881 cttcccataa ttcttcatcg tctgtgccga attcttggac aggaagcaat gtcttgcttt
     2941 tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct agctcgagtg
     3001 ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc ggtatatcat
     3061 atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga ccctcgaata
     3121 catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt tctttgctag
     3181 ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat ccgggtactt
     3241 ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag acggattcaa
     3301 agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa taatcccttg
     3361 aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt cagattcgta
     3421 cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga aactcaatag
     3481 tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct caccttccta
     3541 tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct tcgtgatatg
     3601 tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta ctgtccgcaa
     3661 atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac
     3721 ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga ttccttgccg
     3781 agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt cctttacaag
     3841 taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat cacctaaagg
     3901 aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag aaagatgaag
     3961 atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat acatgacttc
     4021 caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt caatcagctt
     4081 ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg gcgtatcgat
     4141 atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat ggaatcttct
     4201 gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat gcaacttctc
     4261 cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt tctgctcctg
     4321 tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa
     4381 tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca taataatccc
     4441 cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt tccataatct
     4501 gagcaaagaa aaagccgtct agacactcag acggactggt ttctttt
//
LOCUS       DACDEA010000124         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDEA010000124 DACDEA010000000
VERSION     DACDEA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.127"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV136_002641"
     CDS             complement(543..2048)
                     /locus_tag="IV136_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8466564.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV136_002642"
     CDS             complement(2045..2731)
                     /locus_tag="IV136_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8466565.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV136_002643"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV136_002643"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8466566.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDEA010000125         4386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDEA010000125 DACDEA010000000
VERSION     DACDEA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.128"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IV136_002644"
     CDS             complement(65..1285)
                     /locus_tag="IV136_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8466567.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IV136_002645"
     CDS             complement(1305..1910)
                     /locus_tag="IV136_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8466568.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IV136_002646"
     CDS             complement(1959..2891)
                     /locus_tag="IV136_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8466569.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IV136_002647"
     CDS             complement(3312..4172)
                     /locus_tag="IV136_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466570.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4304..>4386)
                     /locus_tag="IV136_002648"
     CDS             complement(4304..>4386)
                     /locus_tag="IV136_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8466571.1"
                     /translation="KKTRKEGTLFGFSVCTEIKVLLGIQA"
BASE COUNT         1250 a          883 c          721 g         1532 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct tggattccca
     4321 ataatacctt gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt
     4381 tcttat
//
LOCUS       DACDEA010000126         4342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDEA010000126 DACDEA010000000
VERSION     DACDEA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.129"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1056)
                     /locus_tag="IV136_002649"
     CDS             complement(<1..1056)
                     /locus_tag="IV136_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8466572.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCI"
     gene            complement(1164..3191)
                     /locus_tag="IV136_002650"
     CDS             complement(1164..3191)
                     /locus_tag="IV136_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8466573.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3336..4067)
                     /locus_tag="IV136_002651"
     CDS             complement(3336..4067)
                     /locus_tag="IV136_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8466574.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1212 a          900 c          716 g         1514 t
ORIGIN      
        1 tatgcataat aaaataaaca ataagaaaag tgaagctact aagatataaa tccaattatt
       61 gtctttttta atcgtaacat ccgtcgtgtt caattccttg gctacttttc cgtctattgt
      121 aaaatctttt tcaaataccc attgattggt atagttgatc gtagtatttc ctttttttct
      181 cgaaaatttt ccattttcat tttcatttcc taaaacagtc attgtaaggt gatattcacc
      241 tggggttagc ggttgaccat ttaaagcagt tggaaaagaa aaatttgtat tcggcgcaat
      301 ctgcaatcct tctttttcct cttgatataa aacttcagaa tgtccttttt ttgtaatttt
      361 tgtttcaata actacttgat tgatataggt tttttgatca ttttgaacgt ttgctaagat
      421 gacatttctt gcattgattt gatctggttt aacttcatga agagtaaggt tcggagcaac
      481 ttcgttcagt gtctgttgca tcaacaaagc aataatatag gtgtattcat tctcaatcga
      541 taagctttga tccttagttt ctgtttggtc cttttccgtc tggatttctt ggaatgtgat
      601 cccaccagcc aatacaccat caaatcgctc attcggcatc ctgacatgaa aagcaacggt
      661 ttggacactt ttaggtttta gcgtcactat ctgtggatag ctcacataat ctaccagatc
      721 aaagatcagg ctcatgtctt tttcaatctc gttttttccg tattcaacca tcacatttga
      781 attggtggtt gcgctattca cagaaatatc gattttcact tctttttctg tatcatttct
      841 caattccgct tttacttcta cttcctgatc cggatcaagt tgaagatcga agtaagtttt
      901 tctcttgtcg atttgattct ctgaagttat cggcgtcact gaaaaattaa atgcactagc
      961 tgacactgat atattgttaa aaagtgcccc taaaataact aggagtgtta taaataattg
     1021 ttgttttggt ctcaagaaaa tcgctccttt aatcatatgt aagagaagac tataacaaaa
     1081 tcttgtctct gtgggacaca agagcctatg gtacctgtgc ccttcttgaa actcaataat
     1141 tttgttatat tcttctcttc ctgttaattt cctgggacat ctgcaagtat ccaagatagt
     1201 ttcccctcaa aggttccgat tctttggttt tctacaggaa tatttattct gattccacga
     1261 tgagctgtag aatctaaaag agaggacaaa tcgctttcac ccttttgatc actttcgaaa
     1321 atcgtaacag cggatgtccc tataaccgtt gtattttcag aggtaacaaa ggaaagagct
     1381 tctggaagct caaaatctcc atttgtaaga actttctctt gtttcaattg taatttccaa
     1441 cttccaaaag cccgttcatc tataattttc aacccacctg aaaactgtcc gaattcactt
     1501 aatggtctac tggatatttt atgttttcca aaattgacat attgaggagc agttgagaga
     1561 taaagtttgc cttttctgta ataaaaatag atatcctctg tattttcgga gaagatgcct
     1621 tctgtattcg ctgaatccga gtcaaaaaca tacccttcaa tatcaatagg agtttcttta
     1681 tacttctctc ctattttccc agtgattgtt ttcatttcag caatatcaat ctgactgtct
     1741 ttatctttat aataaacatg gatatctatc tccttcagct ttgcgatctt taggtttatt
     1801 tcattcgatt gctcaatcaa attcccgttt tcaaggtatt caaaatgcat agtgagtact
     1861 ttttcaggaa ttacttcctg caaagttgtc tccaattcga ttattaacgt atctccagca
     1921 ataaaataat ctgtcagatt tagcgtgact cgatttccgg ttatcaagat atctttggca
     1981 tcaatttgat ttccattgat caagatatgc tcgggaatag ttaattcttg tgggaggtcg
     2041 atactagtct gcattttctt atattcaaga aaatccccct cgaccttcaa tgtatttctg
     2101 atagttatct tatcgtttcc tccagcttca gcgatggccc cataatcact atcgttgact
     2161 tttacatcag ctgttgatgt gacattcgat ggaagtcgcg tgacagcgac agcattttct
     2221 tgaaaaaaag tacctgttga agaagtaaac ccccaattta cttctgtaga accaaactgc
     2281 ctaatgagat caggtataat ccgagaagag cgaccgacac cttccaaatc ataagtaaga
     2341 cttctttgct ccgcgtccca tttaattatt aacaagcgcc atgtgccatt agaaagaaat
     2401 gtaggagcta ccgtcacgcc agtatgctct ccagaataat tattattatt tggtttaggc
     2461 gtgacaaaag cgacatgtcc atgccctcta ttatcagcag ttatctctcg atccattcga
     2521 tcagaagaac cactattttt atacgtatca aactcaatcg agagcgcatt cgcaatgtaa
     2581 ggttggccag aacgagtaga atatgagcca attcccatac ctggcgatcc aataacttga
     2641 ctgggattac tagccattct aggatcgcca gtcaatgtaa aggtcatgcc atctcccgct
     2701 ctatctcgtt gatttcctaa gtataaataa gatttaaatg taaaatccga gagtaaacta
     2761 atcttcttat ttgaccaaat agctcctctt tgagaccttt ctgctgggtt caactgcagt
     2821 aacttcccat tttccaatat ggacgtgcta gtaccgatag gtgtttgaaa aattttatcg
     2881 atagaaataa tattgggatc gtcaggcagc tcaaccgcag aaattggtgc atcttctagt
     2941 ccttgtttgt ccgaaccctc agcttctacg ttgtcagtgg tctctccact actcgaaata
     3001 cttaaatcct cactgctttc ttgtgtctgg ttggattcta ttaaactgtc gaatgtcgaa
     3061 gtgagtttgc tttcttcgtc tcttctattt ccatgctcca actgattcaa ttccaaagag
     3121 taccctatga tcggaaagat attagtcact aagagactgc ttaaaatgag cacgatcctc
     3181 ctctttttca atatatcttt tcctcctgat cgttaaattc ctagatgaac tattcgtatc
     3241 caaacaaatt tagcctagcc tttgacaatt tttgctcgat tacctcgcaa gacaatatga
     3301 ctacgtattt atttttaaca gttcttttca gtaaactagt tgttaggtgt atcttctaat
     3361 gtccaaacta atttagtgga atattgttgc gctaacttga cagatttacc gggtaccgaa
     3421 agctgtaccg catcttgatt ttcattttca tttgcaccaa atcgatagac ccaagtcccc
     3481 acacctgttc ctgatttagc attaattgct aacgtattgt ttcctcccgg tgtcaacgtt
     3541 aattcagaat tcaccgtttg cggcgtatat gttggatcaa ccaaagaaac agcctgccct
     3601 ttcgtaaatt tcagctgtgc tccaactagt tcatccccac tggcagtttt aaactgttct
     3661 ggttgagaga cacttaattt ccacccagat aaggtacttc tcctatctgt cacttggaca
     3721 tatgtcggtt ttctagaccc atcgttcatc acatcagcag ctgcataata cgttttatcc
     3781 gcagtagaaa ttttttgagt cccaaatttg aattttgatc cataatcaat cgataatgcc
     3841 cctcctgttc cagggcttgg aggatctgat ggatcgacag gtttattggg atcaggattc
     3901 tctggatcaa caggaggagt tacaccctca tctcctgctt caaatgtaat cgttccttct
     3961 gtgttatatt cctttgggta tacttcagtt gcagaaacaa aagtcgtact tgctaccatg
     4021 ctgaataaca tcattgctgc tgttaatacg atttcttttt tcatcattga cattcctctt
     4081 tctttattta ctaaacatat cagtcgattg gattaatcca tagcaaaatt ctgttttacg
     4141 atacttatga aataaaagaa catagaccta tatttggtcc tatgtatcga ttagttataa
     4201 atgattatca gcccgatttg atttctagtt tttattgcaa cttgatgcaa acacaatcta
     4261 gaaatacagc tgtcatagcc tttaaaataa ttcgtaagtc cccctcctat ctatcaaaat
     4321 gtgacttgac gagagtgtaa aa
//
LOCUS       DACDEA010000127         4333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDEA010000127 DACDEA010000000
VERSION     DACDEA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.130"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IV136_002652"
     CDS             63..296
                     /locus_tag="IV136_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466575.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IV136_002653"
     CDS             complement(455..577)
                     /locus_tag="IV136_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8466576.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IV136_002654"
     CDS             767..991
                     /locus_tag="IV136_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466577.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IV136_002655"
     CDS             1434..1640
                     /locus_tag="IV136_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8466578.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IV136_002656"
     CDS             1640..1891
                     /locus_tag="IV136_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466579.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IV136_002657"
     CDS             2072..2344
                     /locus_tag="IV136_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466580.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IV136_002658"
     CDS             2789..2968
                     /locus_tag="IV136_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466581.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="IV136_002659"
     CDS             3027..3383
                     /locus_tag="IV136_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466582.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1527 a          675 c          836 g         1295 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt taa
//
LOCUS       DACDEA010000128         4322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDEA010000128 DACDEA010000000
VERSION     DACDEA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.131"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            301..858
                     /locus_tag="IV136_002660"
                     /pseudo
     CDS             301..858
                     /locus_tag="IV136_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            916..2955
                     /locus_tag="IV136_002661"
     CDS             916..2955
                     /locus_tag="IV136_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8466583.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3064..4215)
                     /locus_tag="IV136_002662"
     CDS             complement(3064..4215)
                     /locus_tag="IV136_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8466584.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1566 a          800 c          851 g         1105 t
ORIGIN      
        1 ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt
       61 aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg
      121 tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt
      181 ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc
      241 acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga
      301 ggtcgctcgg attcacttct ggttgcacaa tacaaccaga aaacacaaca agcaaaactg
      361 atctctatca tgagagattc atatgtcgat attccaggtt acggaatgaa taagataaat
      421 gcagcttact cttatggggg agttgattta ttaaatcaaa cattaaagga aaatttcaaa
      481 tttgaagctc cctattatgc aagtatcaca tttcaagatt ttatcgattg cgttaatgaa
      541 ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc tagatttaga tggcgtatat
      601 ataaacaaag gagagcaagt gatggatggt aatactttac tccagtatgc tcgatttcgg
      661 gaagacaaag aaggagactt cgggaggatc agaaggcagc agcaagtgat caaagctatc
      721 tctcagcagc tgaaagacgt aacttcaata ttcaagctac ccaaagcggt tgggaaatta
      781 cttggtagta tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg tatggatttc
      841 ctcaaagaca tatgttagaa taaacaggta taaatagtga aataaaggaa tgacaagcaa
      901 gagaaggagg aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac aggcgcttat
      961 attgccggcg cagtgatctt aatagcagct gcgggtggcg gttattttta ctaccagcac
     1021 tatcaagaaa cccaagcagt agaagctgga gaaaagacag ttgagcaatt tgtccaagct
     1081 ttgaacaagg gagattataa caaagctgca gaaatgacat caaaaaaggc ggcaaataaa
     1141 agtgcattat ctgaaaaaga gatcttagat caataccaga atatatacgg tgctgctgat
     1201 gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa aagacgattc tacttacagt
     1261 ttttcatata aagcaaagat gaatacctca ttaggtgaat tgaaagatct ttcttataaa
     1321 ggaacattag acagaaatga tggtcaaacc acgatcaact ggcaacccaa cttggttttt
     1381 ccagaaatgg aaggaaatga caaagtaagc ctgaccacac aagaagcagc aagagggaat
     1441 attatagatc gaaatggtga accattagca acaacaggca aactaaaaca attaggagtc
     1501 gttccaagta aacttggaga tggaggcgaa aaaacagcca atatcaaagc gattgcttcc
     1561 tccttcgact taacagaaga tgctatcaat caggcgattt cacaaagctg ggtacaaccc
     1621 gattactttg tcccattgaa aatcattgat ggagcaacgc cagaacttcc agctggagct
     1681 accattcaag aagtagacgg cagatattat cctttgggtg aagcagctgc tcagctgatt
     1741 ggttacgtgg gagatatcac agcagaagat attgataaga atccagaatt aagcagtaat
     1801 ggaaaaatcg gtcgctcagg tttggagatg gcttttgata aggatcttcg tgggacaaca
     1861 ggtgggaaat taagcattac agatgcagac ggtgtagaga aaaaagttct gatcgaacat
     1921 gaagttcaaa atggcaaaga tatcaaattg acaatcgatg cgaaggcaca aaaaacagct
     1981 tttgacagtc taggaggaaa agctggatca actgttgcga caacgccaaa aaccggtgat
     2041 cttcttgcgc ttgctagctc tccaagctat gatccaaaca aaatgacaaa cgggatctca
     2101 caagaagatt acaaagctta tgaagaaaat cctgaacatc cattcatcag ccgatttgcg
     2161 acaggttatg ctcctggatc tacgtttaaa ataattacag cagcaatcgg tctcgacaac
     2221 ggcactatcg atccaaatga agtgttgacg atcaacgggc ttaaatggca aaaagacagt
     2281 tcttggggat cttttaaaac aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa
     2341 actgctttga tctattccga taatatatat gcggcacaag aaacgttgaa aatgggtgag
     2401 aaaaaatttc gtacaggctt agataaattc atttttggtg aagaccttga tttgccaatt
     2461 agtatgaatc cagcacaaat ttctaatgaa gatagcttta actcagatat cttgctagct
     2521 gatactggat atggacaggg cgaacttcta attaatccta tccagcaagc agcaatgtat
     2581 tctgttttta ccaacaatgg cacacttgtc tatcctaaat tgattgcaga taaagagaca
     2641 aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga
     2701 gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg gattccgttg
     2761 gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac
     2821 agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag catgttggaa
     2881 aataaagaag atgatgattc agcaaccaaa cgagcatcgg aactattgca atacctcaac
     2941 caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc
     3001 acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt ctttttgatt
     3061 tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag aaataagtgt
     3121 acttaataaa atcacgataa ctaaaggtga atagtactgt ggttcgatca attgactttg
     3181 ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc gaaatcatac cagcaccgac
     3241 catcaatgaa ctatgttgag aaaaaccggc aatctgagca ccaaaataac ctccaagcaa
     3301 tttggtcaaa atcgctacaa gcgtcaatat agaaataaag agtagttgtt cactaaagtc
     3361 agaaaaatca acttctaatc ctacactgac aaaaaatact ggtataaata ctgcatatcc
     3421 taaagcctct acattgtggt atacttcttc tttaaccttt gtctgactga cagctatccc
     3481 agcaaaaaaa gcaccaatga cagagctaag cccaacaaga tccgctacgt aagacattcc
     3541 taaacaaata actagcgata cgatgataat agaagaattt gcgtacactt tttctgcaaa
     3601 tgaaagtagg aaaggcgcaa tccatttgac taataagaaa ataaaaacaa aataaattag
     3661 ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa gcttcccccg ttagaaaaga
     3721 taggctgaaa cttagaacta aaacgactaa aatgtcatct accacagaag cacccaaaat
     3781 tgttgatcct tcttttgtat tcactacgtt tagttctttt aatacttcta ctgaaatact
     3841 gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg aaacttggaa
     3901 tgctttgcct gtcaaccagc caaaaacaac cgggaagatg atcccaagca aagcaacgaa
     3961 cataccaggt cggaaatatt ttttcaaaag agataaatca ctttccagac cagctaaaaa
     4021 cattaataag atcacaccaa tctccgaaaa gtcatggact agaatatcag gtttgaccca
     4081 attcaatcca gcttggccta gcaaaacacc aacaagtaat tgtccaatca ctgctgggat
     4141 acctaatctc cgcgagatat gtgagccaat cgttgttgaa atcaatatca ggcataaaat
     4201 accaataaat tccattctca tcattccttt gtataaaata tagcccgaac ttagcataac
     4261 atacattatt gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc caaaagattt
     4321 tt
//
LOCUS       DACDEA010000129         4304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDEA010000129 DACDEA010000000
VERSION     DACDEA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.132"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            569..1537
                     /locus_tag="IV136_002663"
     CDS             569..1537
                     /locus_tag="IV136_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8466585.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1548..2363
                     /locus_tag="IV136_002664"
     CDS             1548..2363
                     /locus_tag="IV136_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8466586.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2431..3141
                     /locus_tag="IV136_002665"
     CDS             2431..3141
                     /locus_tag="IV136_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466587.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3177..>4304
                     /locus_tag="IV136_002666"
     CDS             3177..>4304
                     /locus_tag="IV136_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8466588.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1447 a          644 c          904 g         1309 t
ORIGIN      
        1 tctgaatata actactgtat caaaaacaat cgattatgag acaaaacaaa atgacctctt
       61 ttatttaaga aaggaggtca ttttttgtat cataaattgt attaaaatca atgtatcact
      121 ttttaatcag ttttaagacc ataaattgcg tactaaaaga aattctgcaa ttcaaaataa
      181 agcattttaa gccattttca aatttaagtg cacccatcta cccgtcaaat cactttattt
      241 tccactccag tagtttccta attcaactgt gtgcaaaaac gatttcttct aaacaaatca
      301 agacaaactt ataaaccttc atcccagcac cataattatt taaatcatag gcatttttcg
      361 aaattagtat atgtactcaa gtttcactgc agattattta tattttttag agaaataaat
      421 ttttcctatt gacaaacaca aagaaagcgg atacaatgca attatgtcac atgacgttat
      481 atgacgttat gtgatatctt tacatttaat cagttacttt tattttggat aaaattaaca
      541 tttttaaaat tttaaggagg aaatgttgat ggatgtagca aaaacggtta acgcaattgt
      601 agaaaaaaat gatggagaga tttctggaat ttatttggtg gcttgtggcg gttcgctggt
      661 agatatgtat gtctcagatt attttcttaa agccgaagca tcgaagtgta ttacaggctt
      721 atatacagcg aacgaattta accatgtaac cccaaagcaa ttagatgata aatctgttgt
      781 cattctatgt tctcatggag gaaatacagc agaaacggta aaagcaggag aagtcgctca
      841 agctagagga gctgtgactg ttggtctgac tcataataaa actgcggatt tattaaaggt
      901 ttctgatcat agtttccttt acgaatgggg agatgatgca aaaatagtca ataatcctat
      961 ggccatcatt cttgatttaa ctgtatcttt ggtcaatgtt accgaaacat ttgaacatta
     1021 cggtaaattc agagaaggca tgaaggttat cgataccgta attgaaaatg gcattaagca
     1081 ggtgcaggat cgtattaaag tgtttgctga taagtacaaa gatgaaacaa tgttttatat
     1141 cttaggtagt ggaccatcat atggacatgc ttatggtttt tcaatttgct ctttgatgga
     1201 aatgcaatgg cttgattcaa cttctgttca ttctggagaa ttcttccatg gaccttttga
     1261 agtaacagac aaagatacga attttattct cttgatgagc attggacgca ctcgtgcttt
     1321 agatgaacgg gcaaaacgat tccttgataa atatgcagaa agagtagaaa tcgttgatgc
     1381 aaaggaacta ggattggata tgatatcaga tgaagtttct gaattcttca atcccatttt
     1441 attctacagt attctttcag aatatcgttc agctttagca gatgtacgca atcatgattt
     1501 agatgttcgt cgctatatgg ggaaagtgga gtactaggag gattaaaatg aaggtattag
     1561 gcttaggtga taacgtcgtc gataaatatg aaaatctggg aattatgtat cctggaggaa
     1621 attcattgaa ttttgcagtt tttgcaaaaa aactgggaca tgaaagtagc tttatgggag
     1681 tttttggctc agatgaggag gcagctcatg ttttatcagc aataaaagaa ataggactgg
     1741 acaacagtca ctctcgcttt gaaaaaggcg agaatggctg tgccagagtg aagattgatg
     1801 agggcgaccg gattttcctt ggcagcaatt cgggaggtgt tactcgtgaa tacccgattc
     1861 agcttactga agaagacaga gaatatttaa accagtttga attgattcat atgggattat
     1921 atagccatgt caatcatcta ttagaagagt tacaaaatgt ttctggaaag atttcttatg
     1981 atttttctga cgactttacg gaagaagagg ttcagcgggc aattgataaa gttgattttg
     2041 gcttcttttc tatagaagac ttttccgatg aagaagtaaa gcagtttttg atgaagcatt
     2101 tttgtgagcg aaatgatgta ttgattgcaa cccgaggaga aaaaacggct attggttatg
     2161 atggagcaaa gttttatgaa gtgaaccccg tgctagaaga accagttgat acaatggcag
     2221 cgggagattc tttcttaaca gctttcctac tttattttct cgaaggggac gatatagtca
     2281 ccgcgatgaa aaaggggaat gaatttgctg ggaagtcttg tctggttgac ggttcgttcg
     2341 gctttggtat tcattacgat tgagcttgtt ttaaattcct gcatttgata acctaaagaa
     2401 agaaatcccg gtaaagtgga ggttaaaaaa atgctggata caaatgcaca attaccattg
     2461 tacttacaat taaaagaaat attaaaaaat aaaatcaata caggggagta tcccgagaat
     2521 acgaaaattc ccgctgaatc agagctgata gaaagttatg aggtcagccg aatcacagtg
     2581 agaaaagcag tagaagaact ggtagaagaa gggtacctac ttaaaaaaca agggaaagga
     2641 acttttgtca accggcataa agtttttcgt aaaattgaat atattatggg tttcagtgag
     2701 agttgtaaat ctaatggttt tacaccgact agcattgttt tggaacgcaa gatcattcca
     2761 gcaactgaag ggatggcaaa aaaactcgat atcgctcaag gtgatgatgt tttatatatc
     2821 cagagaaagc gaatggcgga taacatcccc attatgttag aaaacaatta tttttcttat
     2881 gaaagatata gtttcttaat ggaggaagag ctgactgatt ctttgtatga agtacttaaa
     2941 gtccaaggta ccttagccat caatcctggg gaaactgcac ttgaaattgt cacagctgat
     3001 gaccaaattg ccaaaataat ggaagtagct gttgggacgc cctttttctt tatggatact
     3061 ttaatcaacg atcaaaacaa cataccaatt catgtaggta atcaatatta cctgggagaa
     3121 gtatatcgtt tttacttatg atttcctgtt tggatttcaa ggagtagatt ttatttatga
     3181 agcaaatatt gcggtcaaaa atgatttttg atggagagca gccacctttc aaaggttata
     3241 ttgtaatcaa tgaagggatc attgaaaagg tggaaaacaa ctggaattac agtgatctat
     3301 taaatgaaaa cacggaatta attgcttatg atgatcaatt cgttatgcca gcaattcatg
     3361 ataatcatgt atttttcagt ggttatttgg ctatgaatgc tggcatagat ttaagcgata
     3421 catcttcagc taacgaggct gtagagcgta ttaaagctgt aatgaaccat aaaaaagcag
     3481 atgaaccaat ttatgctcat ggctgggatg ctgccctgtg gaaaactgac ccatcagaag
     3541 agctattgaa tgaaataaac cactttggtc caatcacagc gatcgacaaa aatcgcagcc
     3601 attgttggat gaatgctatt gctaagcaga cttatggttt tacagaaaaa gaaaccagtg
     3661 ctgaaagtcg agtattatta attaaggaaa tgctagcaaa caaaaagctt ttagcagagg
     3721 tttacgctga atttgaagca ttactgcttt caaaaggtgt tatatcaatg aaagaaatcg
     3781 tttttgatga cgcagaattt ctttctaata tacctcagag gaaaatactt agtaacctat
     3841 atgtgcaggc agtccaagaa cctctacagc atgaacttct atatgagtat aaggaaaaag
     3901 ttttccctga aaaggtccgc tttggtggag tgaagatcat ggtagatggt gttgttgcgg
     3961 atagaagtgg agatatttac ggtgtttatc agtcaggagt agcttcaccc gagattgact
     4021 atgaggcaat taaagcagaa gttgctgcat tcaatgaagt agagattcct tgctgtttga
     4081 caacggaagg agataaagcg ggttataaag ctgcgtcaat tcttgtcgaa tttggaaagc
     4141 ggttacctga aggcgttttt aattcgatta gtgatttgga aatggttact caaaagacag
     4201 cagaaaaaat gaacgaagga caagtagtag cagaggtata tccacaaatt ctcggcttga
     4261 atcaaacgtt agaagaggct tacatgtcaa ctgttataaa aaat
//
LOCUS       DACDEA010000130         4157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDEA010000130 DACDEA010000000
VERSION     DACDEA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.134"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IV136_002667"
     CDS             179..775
                     /locus_tag="IV136_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8466589.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IV136_002668"
     CDS             899..2698
                     /locus_tag="IV136_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8466590.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IV136_002669"
     CDS             complement(2976..3188)
                     /locus_tag="IV136_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466591.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IV136_002670"
     CDS             complement(4052..>4157)
                     /locus_tag="IV136_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466592.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACDEA010000131         3950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDEA010000131 DACDEA010000000
VERSION     DACDEA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.135"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..616)
                     /locus_tag="IV136_002671"
     CDS             complement(74..616)
                     /locus_tag="IV136_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8466593.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="IV136_002672"
     CDS             751..1140
                     /locus_tag="IV136_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466594.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="IV136_002673"
     CDS             1144..1908
                     /locus_tag="IV136_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8466595.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2037..2984
                     /locus_tag="IV136_002674"
     CDS             2037..2984
                     /locus_tag="IV136_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8466596.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3114..3854
                     /locus_tag="IV136_002675"
     CDS             3114..3854
                     /locus_tag="IV136_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466597.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1310 a          676 c          802 g         1162 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     2941 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     3001 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     3061 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     3121 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     3181 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     3241 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     3301 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
     3361 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
     3421 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
     3481 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
     3541 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
     3601 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
     3661 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
     3721 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
     3781 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
     3841 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
     3901 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
//
LOCUS       DACDEA010000132         3751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDEA010000132 DACDEA010000000
VERSION     DACDEA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.136"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..228
                     /locus_tag="IV136_002676"
     CDS             <1..228
                     /locus_tag="IV136_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8466598.1"
                     /translation="ILAAVDKHTDLNEMIKTGKFGYCSEHGDLNAFLSNMERLKDEKI
                     RNELGKNAFIYLKNNFDVKRSYSIIMNHFEL"
     gene            244..1266
                     /locus_tag="IV136_002677"
     CDS             244..1266
                     /locus_tag="IV136_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8466599.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            1263..2372
                     /locus_tag="IV136_002678"
     CDS             1263..2372
                     /locus_tag="IV136_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAP8466600.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            2376..3500
                     /gene="wecB"
                     /locus_tag="IV136_002679"
     CDS             2376..3500
                     /gene="wecB"
                     /locus_tag="IV136_002679"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP8466601.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            3497..>3751
                     /locus_tag="IV136_002680"
     CDS             3497..>3751
                     /locus_tag="IV136_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8466602.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIY"
BASE COUNT         1367 a          575 c          760 g         1049 t
ORIGIN      
        1 attttagctg ctgtagacaa gcatactgat ttaaatgaaa tgataaagac tggaaaattt
       61 gggtattgtt ctgaacatgg agatttaaac gcatttctaa gtaatatgga gcgattaaag
      121 gatgaaaaaa taagaaacga attaggtaaa aatgctttta tttatttgaa aaataatttt
      181 gatgtaaaaa gaagctattc tataattatg aatcattttg aattataaaa aggagcaatt
      241 taaatgttca aagataaaat tttattaatc acaggcggca cgggttcttt tgggaacgca
      301 gtgatgaaac gctttttaga ttcagatatc aaagaaatac gtatcttttc tcgtgacgag
      361 aaaaaacaag acgatatgcg gaaaaaatac aataatgaca aattgaagtt ttacattgga
      421 gatgttcggg acatcaacag tgtcaaaaat gcgatgtacg gcgttgatta tgtcttccat
      481 gcagcagcac ttaaacaagt accttcttgt gagtttttcc ctatggaagc tgtgaaaaca
      541 aatatcatgg gaacagataa tgtcgtaact gcagcaatcg aaatggggat tcaaaaagtt
      601 atttgcttgt ccactgataa agctgcttat ccaatcaatg ctatggggat ttctaaggct
      661 atgatggaaa aagtatttgt tgctaaagca agaaccgtta ctgacgaacg cacgatgatt
      721 tgtggcacac gttatggaaa tgtaatggct tctagaggat cagttatacc attattcatt
      781 gaccaaataa aaaatggtaa accattaaca gtgacagatc ctggcatgac aagatttttg
      841 atgagtttgg aagaagctgt agagttagtt gtttttgcct tccaaaacgc aaaagcaggg
      901 gatatcatgg ttcaaaaatc accagcttct actgttggcg acctagcgca agccatcaaa
      961 gaattattcc atgcagacaa tgagattcat atcatcggaa cgcgacatgg cgaaaagcgt
     1021 tatgaaacac tactgacaaa agaagaatac ttagttgctc aagatatggg tgatttctat
     1081 cgtgttcctg cagatacacg tgatttgaat tatgacaagt atttcgttga aggaagcaaa
     1141 gagttgacac aagaaggaga atacaattca gataatacac aacgtttatc tgtagaacaa
     1201 atcaaagaaa gacttcttga actggactat gtaagaaaag aattgaaaga gtggcaaaat
     1261 caatgaatat tttagtaact gggtcaaatg gttttattgg aaaaaattta gttgctgaat
     1321 taaaaaatga aggattcaca gacattctta gttttgatag agacacgcct gtacatttat
     1381 tggatgaata ttgcgaaaaa gcagactttg tttttcattt agctggagtg aacagaccaa
     1441 aagacgatgc tgaattcatg acaggaaatt ttggttttac ttcagaattg ctagaaaaac
     1501 taaaagagca tggcaataca tgtcctgtta tgatttcctc ttctatccaa gcagaactta
     1561 ataattctta tggcaagagc aaaaaagccg gagaagatct attaaagaaa taccaaaaag
     1621 aaacaggagc acctgtatat atttatcgct tctcaaactt atacggtaag tggtcgcaac
     1681 caaattataa tacagttgta gccacatttt gttacaaaat cgcaagggac gaagaagtta
     1741 cggtaaatga tccgtctgct atcatagagc tttgttatat cgatgatgtc gtgaaggaac
     1801 tactccattg tctgaacgga caaccttata cagaaaatgg gtattgtgct gttcgtgaaa
     1861 cggatgaagt aacggtcggc gaattaaaag aactaattac atctttcaaa aatagtcgaa
     1921 cgaatttgag tgttcctaat cttgcgagaa gagtagaaaa aaatctgtac agtacgtact
     1981 taagcttctt accagaagac gaattttctt attttctaaa aatgaacgta gacaatcgag
     2041 gatcgtttac tgaattttta agaacaaaag accgcggtca agtatctgtc aatatttcca
     2101 aaccgggaat cactaaagga aaccattggc atcatacaaa aaatgaaaaa ttcttagtag
     2161 taagcggaga aggtgtaatc cgttttagaa aagtagactc aaatgaaata atcgagtatt
     2221 ttgtttcagg agaaaaatta gaagtagttg atattccagt aggttatacg cacaatatcg
     2281 agaatctggg atcaacggat atggtgacag tgatgtgggt aaatgagcca tttgatccag
     2341 aacatcctga tacttattat gtggaggtct aatcaatgaa aaaattaaaa gtgatgacag
     2401 tagtaggaac ccgaccagaa atcattcgtt tgtctgctgt tatcaaccgt ttggatcgat
     2461 cagaagcaat cgatcatatt ttagtacaca ctggccaaaa ctatgactat gagctaaatg
     2521 aagtattctt cgaagacttc aagctaaaga aacccgatta ttttctaaat gcagcaactg
     2581 gtacagcgat tgaaacagtt ggaaatattt tgatcaagat cgatccaatt ttggaagaag
     2641 taaaaccaga tgcattctta gtattagggg atacaaatag ctgtttatgt gcaattgcag
     2701 ccaaacgtcg ccatattcct atttttcata tggaggcagg aaatcgctgt ttcgaccaac
     2761 gagtaccgga agaaacaaat cgtaaaattg ttgatcatac agctgatatt aatctaacct
     2821 atagcgatat cgcgagagaa tatctcttac gtgaaggatt accagctgat cgtgtaatca
     2881 aaacaggaag tccgatgttt gaagttctca attcaagaaa agatgacatc caacattccg
     2941 atgtccttaa tcgcttaggg ttgaaagaag gacaatattt tgttgtttct gctcaccgcg
     3001 aagaaaatat cagctcagaa acaaatttct tacacttggt agatagcttg aatacaatcg
     3061 ctgaaactta ccaattgcca atcatcatca gtacgcatcc gcgaacaatg aagatgatcg
     3121 aagcaaaaga aatcaaattc catgagctga tccaaacaat gaaaccaatg gggttcaatg
     3181 attacaataa attgcaaatc aatgcaaaag ctgtactaag cgacagcgga acgattagcg
     3241 aagaatcttc cattttgaat ttcaaagcat taaacatccg tcaagcacat gaacgaccag
     3301 aagcaatgga agaagcaagt gtcatgatgg taggattaga aaaagaaaga atcatgcaag
     3361 gactggaagt attggaaacg cagcaaaaag atactttgcg acacgtcgca gattattcaa
     3421 tgccaaatgt ttcggaaaaa gtattgcgta tcatcctttc gtatacagac tatgtgaata
     3481 gagtggtgtg gggaagatga gaattgttgt aaatgatatc gctgcttcag aaggtggggc
     3541 aatgtcagta ttgagagact tacattctga tataataagt agtggtgatg aaaatgaatg
     3601 gattttttta ctaggtgaaa aattcataga agaaagtgac aatataaagg tactaacttt
     3661 tcctaaaata aaaactagct ggattaaaag attggatttt gattttatta gtggaaaaaa
     3721 aattatcaat aaattacagc ctgatatcta t
//
LOCUS       DACDEA010000133         3663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDEA010000133 DACDEA010000000
VERSION     DACDEA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.137"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IV136_002681"
     CDS             <1..276
                     /locus_tag="IV136_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP8466603.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IV136_002682"
     CDS             419..640
                     /locus_tag="IV136_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466604.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IV136_002683"
     CDS             656..1498
                     /locus_tag="IV136_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466605.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IV136_002684"
     CDS             1561..3363
                     /locus_tag="IV136_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP8466606.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="IV136_002685"
     CDS             complement(3422..>3663)
                     /locus_tag="IV136_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8466607.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DACDEA010000134         3634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDEA010000134 DACDEA010000000
VERSION     DACDEA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.138"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            124..2121
                     /locus_tag="IV136_002686"
     CDS             124..2121
                     /locus_tag="IV136_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466608.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2197..2532)
                     /locus_tag="IV136_002687"
     CDS             complement(2197..2532)
                     /locus_tag="IV136_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466609.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2659..2874
                     /locus_tag="IV136_002688"
     CDS             2659..2874
                     /locus_tag="IV136_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466610.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2911..3273
                     /locus_tag="IV136_002689"
     CDS             2911..3273
                     /locus_tag="IV136_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8466611.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3319..>3634
                     /locus_tag="IV136_002690"
     CDS             3319..>3634
                     /locus_tag="IV136_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466612.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVL"
BASE COUNT         1318 a          468 c          726 g         1122 t
ORIGIN      
        1 gtttcatgga acggtataaa ggttgagtaa acaaataaga ctaaaacata tagtaataaa
       61 gagtttgttt ttattatctg tatgttttag tctttaacag aattttataa ggaggaaaca
      121 caaatggaaa catcaatgat taatattcca atttatgtag atcaagaaaa attattagag
      181 aagccagtta caaaaaaaga aaaaactgat gtaagagata ggtgtttaga taactataga
      241 gaagttacaa gagatgagtt tgtagagata ctaaattcat cacaatcatt tattccaagc
      301 aagacaaaga ataaaggcaa tgacaaggag agttttgtgg aaaccagagt aattattctg
      361 gatgtcgata acactgttaa agatgagaag gggaaggtag tagatttgag taaagatgat
      421 agtagatact taagcataga aaaggtttta tctatcaatt tagttcataa ttctgcgttt
      481 gcgatacaaa aaagtattcg gtactctgaa aatctagaaa aatacaagat tgtctttcta
      541 ttaaaagaag caattactga ctatggtaaa atggttgctg tttacgaata cttgaaaaag
      601 aacatccccg ggtgtgatga caatgtaaat gcttcgaata gaatgttttt tggaggatat
      661 aagtcagatg cagtgataat aattaatgaa gacaacatgt tagatattac tgagcttccg
      721 attgatttta ccatgacatg ttctaaaaat tgtcaaagtg attcagggag agagtatgaa
      781 ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg acgacaaaga agaaatgaaa
      841 cagtggttta aagactgcct gtttgattcc tccgacatgt cttttaatga gatttatgaa
      901 agattgttaa ccattgatat gaataaatta cttaagtcaa gtaaaaactt aagatgtctg
      961 tttcacaacg atcataatcc ctctgctagt atttttacca gtaaaactgg gcattccatt
     1021 ttttattgtc attccagtaa ttgtgatatc agtgaaaatt ttattggagt tgtgatgcta
     1081 attttaaaga aagacagtcg ggtagaaaca tttaattggc tattagagaa cctagacttg
     1141 tatcctacac attttcgaaa attgaaagag gaatcaaaag tactttttga cactcttgaa
     1201 tcaatgaaag acaaactttt caggacaata aggaattatc tagaggaaat gcgacgtgtc
     1261 tatgatgtct tgctttctga tgttaatttt tttgatgata gtaaggaaag tgtgacttgt
     1321 attttgtcgg gggaacaatt agctaaaaaa atgagttctt actatggtaa aacttatgac
     1381 atagataaat gtaataagtt actatcactt atgactttta taggattgat aaaaaaatta
     1441 gatgatgaag atgttccttt gaagatgctc gaatacctaa ataggttaaa ggaagagaaa
     1501 gcgttaggaa actttaatca taagagaagc aatgtctatc gattagacgt ttttaatgca
     1561 acttctgtga ttcaaaaatt gaatgaagat atccttccac ttctggaaaa aattcatttt
     1621 acatatcaat atttttctta tgaatgggtt aagatatgtt ttaacgaaaa agaggctaaa
     1681 agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg aaaaacaatt gattttagat
     1741 gaagcacatg aagttttaca gggcttatcg tggcaaggga catatctaat gacagagaaa
     1801 gaacttatta acactgtttg tgaacgccta cgtaatgttg gggataacag cgtgaaaaat
     1861 gctttaataa aaaacagagg ctactttgtt aaacaaggat tcactcttga acgagcttct
     1921 aaggatatta agaaactata caatgtgaaa gcaagtagta gttttttaat atatttagcg
     1981 aaaggggcag ggatagatga aacaataaaa gaattgatat tatcaggagc tgggatagtt
     2041 cctctgtatc aatttacaag tgtaacaaga gatgatggac ggagagtaag gaagagaaaa
     2101 gacccaattg tattaaacta gacaggttat ccatagctct taatcttatt tcatctcaat
     2161 acacccaccg ttaaagttcg gtgggttatt tatttttcaa tctttcaata gctgattgcg
     2221 ttcattttct tgccttttta atagcttcat atagtcaatt gttcgaactg catcttcagt
     2281 gctaagagaa cgatagagag ccaaaaactc ttcctcataa tctacgacaa cattgtctag
     2341 aaaatggctt acaggggttc ctagggctct agaaagagct gttaagctat cgaggttagg
     2401 ctctgttaca gaattttccc atcttgctac tgcctgttgt gtagagaaga ttttttcagc
     2461 taattctttt tgtgtccacc cgagagcttt tcgcttttct ctaatgacct ttgcatattg
     2521 tttctgtttc attaatacac ccccatctca gtatagtaca acaagttgtt gtatttgtaa
     2581 tggtatttga agttttttat attttatgag ttgaaataca tcatattgtt gtatataata
     2641 aaacaggagg ggagaatcat gaaaaaagta agtaatctca gccgatacag aaaagaaaaa
     2701 ggactttctc aaacggagtt agcaaaaaaa atgaatgtta cacagcaatg tgtaagttcg
     2761 tggcaaacag ggagaacgat tcctaaaccc tatcaaatga agatgctttc tgagatatta
     2821 agtgtaccaa tgaatgagct gttttctgat gtatttaata aagtaaacag ttaactaata
     2881 agcaattgaa tgattttagg aggagaagac atgtattgta gaaaatgtgg caaagaaata
     2941 acagatgaaa gtaagtattg ccagttttgt ggagcactac aagaaacaga tgagacaacc
     3001 ggaacagaca cagtaaagaa aagtactgag cagacaatta atgaaatgaa ggaacaaatc
     3061 gtacatggac aagagacagg gaagctgtat ttgattattg gttggatttc tatggtagta
     3121 tcgttagcat ttattcctgt tgtatttggt gctgttgctg ttattatggg ctacttatat
     3181 cgagaaaaag atgagaaaat gggtacgatt ttgatgatag caggggtagc tggagcaatc
     3241 tttggtgtac ttttagggat gtcaatgtac tgataagagg gataagaaca aattattttg
     3301 gagataggag ataaaaagtt gaataaagta aatatgaaca agaagtttgt cattataggt
     3361 attgttgtct tggctattat aggtattatt ttttcaaaaa tgacaggtac atctggatta
     3421 gagaagtcat caaaaccatt ggtaacacag attttacaag aacagtacaa cttaaatcga
     3481 tcatgtgatg atgtgacgat tactcatgaa aatggagata acacataccg ggctaaagct
     3541 atattagaca atggaagtgc tatcaacata aatattgagt actatccaaa gaaagaccgt
     3601 atatatgtgg agataccata tgctgaggtg ttaa
//
LOCUS       DACDEA010000135         3549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDEA010000135 DACDEA010000000
VERSION     DACDEA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.139"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..886
                     /locus_tag="IV136_002691"
     CDS             <1..886
                     /locus_tag="IV136_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8466613.1"
                     /translation="LQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVSS
                     AITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHY
                     TNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDT
                     LEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDN
                     LNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLF
                     GKGFGNYDQDIKLKVYYS"
     gene            complement(959..1783)
                     /gene="nadE"
                     /locus_tag="IV136_002692"
     CDS             complement(959..1783)
                     /gene="nadE"
                     /locus_tag="IV136_002692"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8466614.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1795..3267)
                     /locus_tag="IV136_002693"
     CDS             complement(1795..3267)
                     /locus_tag="IV136_002693"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8466615.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1009 a          744 c          631 g         1165 t
ORIGIN      
        1 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
       61 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
      121 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
      181 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
      241 tagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
      301 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
      361 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
      421 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
      481 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
      541 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
      601 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
      661 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
      721 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
      781 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
      841 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
      901 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
      961 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
     1021 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
     1081 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
     1141 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
     1201 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
     1261 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
     1321 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
     1381 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
     1441 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
     1501 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
     1561 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
     1621 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
     1681 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
     1741 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
     1801 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
     1861 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
     1921 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
     1981 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
     2041 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
     2101 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
     2161 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
     2221 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
     2281 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
     2341 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
     2401 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
     2461 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
     2521 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
     2581 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
     2641 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
     2701 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
     2761 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
     2821 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
     2881 tgtttctact aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc
     2941 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
     3001 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
     3061 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
     3121 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
     3181 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
     3241 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
     3301 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
     3361 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
     3421 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
     3481 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
     3541 atcgtagcc
//
LOCUS       DACDEA010000136         3522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDEA010000136 DACDEA010000000
VERSION     DACDEA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.140"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..1398
                     /locus_tag="IV136_002694"
     CDS             7..1398
                     /locus_tag="IV136_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8466616.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IV136_002695"
     CDS             1414..2454
                     /locus_tag="IV136_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8466617.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..>3522
                     /locus_tag="IV136_002696"
     CDS             2473..>3522
                     /locus_tag="IV136_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8466618.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTT"
BASE COUNT         1254 a          518 c          658 g         1092 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaagcat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ct
//
LOCUS       DACDEA010000137         3480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDEA010000137 DACDEA010000000
VERSION     DACDEA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.141"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..999
                     /locus_tag="IV136_002697"
                     /pseudo
     CDS             1..999
                     /locus_tag="IV136_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1100..1672
                     /locus_tag="IV136_002698"
     CDS             1100..1672
                     /locus_tag="IV136_002698"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8466619.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(2145..2717)
                     /locus_tag="IV136_002699"
     CDS             complement(2145..2717)
                     /locus_tag="IV136_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466620.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2733..3338)
                     /locus_tag="IV136_002700"
     CDS             complement(2733..3338)
                     /locus_tag="IV136_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8466621.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1141 a          672 c          583 g         1084 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccatca tttcaaaaga
     1141 gaatctctta aatcaggact tttctactga aacgatctgt gagaaatggg cagctgacat
     1201 tacgtacatt cctactaaga aaaatggttg gtgttactta tcttcaatca tggatttaca
     1261 cacgaaaaga attattagtt atacattttc aaaacgaatg actgtggatt gtgtcattca
     1321 aacattgaat aaagcaaaaa tacattatca cattccagaa ggaatgattc tacacactga
     1381 cttgggcagc caatacacag caagagaagt ggaacaatgg cttaaaacca acaaaataag
     1441 gcattcttat agtagaaaag gaacacctta tgataatgct ggaatcgaat ctttccatgc
     1501 ctcattgaaa aaagaagaag tctacacgac tagctactca gattttgaag aagcaaatcg
     1561 agcgctattt agctacattg aaggatttta taaccgaaat cgaatccata gctcgattca
     1621 ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct
     1681 actaaaatgt gtccaagata ttgactcaaa tccagtaata tcacaatagg aatgctgttt
     1741 attatattag cagcagtgct gatttttatg atagttattt ggttcttaaa tgaaagatct
     1801 aaagacaatc aacaccgtag ggatatggag ataaaatcaa tagagtcaat agattattta
     1861 tcgataataa cactaaaaaa gccacgttcc taaacgtgac ttacgctgaa cgatatgtgt
     1921 tgtttggtag acgaacaaat cgttcattat aaatatatga tggtatccta atatagtaag
     1981 cgtggctagc atacctttaa ttactttacc ttttacagtt accatacaca ttttttgagc
     2041 agaaatcaag taaataaaaa aatattatat ttcacaacat gctaatagac tcatatctag
     2101 aacgcattaa gtatttacct gtctggttcg ctgagacttc ttttttaact ttcttcaatt
     2161 ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg ttatagcttc aacttcctta
     2221 tacgatttac ctgaagtaag cagttcatat gcgtgcctct tcttcggtgt gattgttgct
     2281 ttcggacgtc cttctctgaa attaggatta ttctttcttg caaacatttt tccttcttgg
     2341 gtacgttcca caatcatatc acgttccatt tctgctacac tcaacaacgt ccgtgtcacc
     2401 attcgcccca tggacgtatt ttcaatcgtt cctagattca aaaccttgat cgttacgtta
     2461 tcatcgagta aaggttcaat gatctctaat gcttctctcg tgttacgagc aaatcgatca
     2521 agcttcgtaa caactatttc atctccaggc ttcagaccag ccaacagctg attgaatttt
     2581 ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa tattcttctt ctctacgcag
     2641 ttctcaagca actgttgaat ttgttctttt gtactttgag aagcggttga gacacgtgca
     2701 tatccatatt tcgtcatttg gtccacctac ttttacaaat catctatcgt atactcagaa
     2761 tacccttcat agaaatagct gacatcgatt cctttcatat ataactcgcg atcgtcaatt
     2821 ttatcagtca gagcattgga aattagatag cgtatttcca aatcgtttat gggacttcgc
     2881 tccattgcag aaagatagtc tgctttatcg attaagttcc aatctacaac tttttgcaat
     2941 tcttccttta aaatgagatc cagccaaatt ctagtacttc ggccatttcc ttctcgaaat
     3001 gggtgagcga tattcatctc cacatatttc ttgataatct cttcaaaaga agtttgtggc
     3061 atctgatcaa tatgctgtaa cgaatgttct aaatacataa ctggtgcaaa tcgaaaattt
     3121 ccttttgcaa tattgaccga tcggattttt ccggcaaaat cgtaaatatc cgaaaataga
     3181 aattcatgaa tttcagataa acctttaaat gtaccaactt cgatttcatt tatttttcca
     3241 caatcgtaca attctttcgc tttttgttta ctgagttttt cttcaacctt agccaactca
     3301 acttgattgg ttaatcccaa tttattctct aaaatcatgc gaaatctcct taactttatg
     3361 acataagttt atgaaacact ctcaacccca ttatatcgcg attttgttta tgtgtcaaaa
     3421 actattaagt ttctgacccc cgatagattt ttcaagtgtc tctattttgc acagtctagt
//
LOCUS       DACDEA010000138         3473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDEA010000138 DACDEA010000000
VERSION     DACDEA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.142"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1160)
                     /locus_tag="IV136_002701"
     CDS             complement(<1..1160)
                     /locus_tag="IV136_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8466622.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVT"
     gene            complement(1163..3412)
                     /locus_tag="IV136_002702"
     CDS             complement(1163..3412)
                     /locus_tag="IV136_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8466623.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
BASE COUNT         1128 a          651 c          498 g         1196 t
ORIGIN      
        1 gttactctag tatttttttt atttgtcgca aaaaatgtta caaataggat cacaataaat
       61 attcctgcga atacaaaaaa tggagcagtc aaatttgtac ttcttgttag aaattgtgct
      121 aattgcatag caataggagc tataaaatta ccaatattac agccaataaa aatcaatgaa
      181 gtagcaaaat taattgtatt ctttgtagtg actttatcta agttactaaa gataaatggg
      241 aagcaccatg atcctgttat acttgataaa aataaacccg ttagcaggaa cgtcatatta
      301 ccgtttgaca cagcaattaa caaatttgaa attataaaga atataattcc caagtatagg
      361 gttccttttc ctatccactt atttattggt ccaaacataa atcctgctag tattcctaag
      421 ataggcatta gagctaaata gttactagca ttaaagttta cccctttaat ttcagttgca
      481 attgatgcaa atcgtacaga aatagcaata ctattcatta cgagtaagat tgcaaataga
      541 actagggcaa aaaccattgg attcatcttt tcttttacat gattttcagg tatgtcacta
      601 ttttcatctc taatttcagg aacttttagc gcgaataaga gagctacagg aaacgcaatt
      661 gcatagacta aaaacgaagc cgtccaatta atgttcacta aaactccagc aagaaaaata
      721 aggattgtct gacctaaagc ttccattgaa tttctaatac ctaatagcgt tgctctagta
      781 ttgccagagt ataaagagct gatatatgtc acagccaatg aattatatac tcctaaaccg
      841 gcacctaata ttactcgact aataagaacg gtttggaagg tagctgtaaa tactggaata
      901 attcctccta taccagctaa taataaacct aaaattattg tctttttcat tcctattctt
      961 ttggcaaagt aactagataa taagataaac aaaataacag ttatacttgg tgcagttcct
     1021 aaaagttcac tttgactttg tgatatcccc aaagactgtt tcatttgtgg aataacacca
     1081 ttaattgctt gagagcttgt taacattagt gaaatactta agattgctcc tttttgaagt
     1141 aagctttttt ggttattcat ctctactttt cctcctgtgt tataacccaa atcttggaat
     1201 caaaatcaaa tgtttctggg cgtcgattct gatcatttat ttgtccacta gatgcatctg
     1261 aacagactag tcctgctctc atcaattcca ttccagatcg caaactattt tgtcctacat
     1321 tataaagtcc gtcattatct aaccctttta atttcagtcg gcggtaagga gcatttacct
     1381 catttaatgt tttataatga gcaacaatag ccgttttctt atcatgacta acaaccatcc
     1441 acgcggtttg attatcatca tcgaatggtg ataatagtct ataaaaagta ccattatgaa
     1501 ttatggttct atatttttta taaaaggcaa tttgttcttt tacaatcctc aaatcgcctt
     1561 cacttaattc atttaagtca agctcatatc caaatgcacc aaaaaaagct acatttcctc
     1621 tagtttttat aggtgttata cggtttgttt gatgatttgg aacaattgat acatgagcac
     1681 caatagacga aagaggatac agcatactag ttccatattg aatttttaac ctttcaattg
     1741 catctgaatc atcactagcc caagcttgag gtgcatagtg aagcattcca ggatcaaagc
     1801 gcccaccacc tgatgaacaa gactcaaata gtatgttagg atattttgtt ataagttttt
     1861 catataagcg atatacgcct agtacatatc tgtggaatac ttctccttgt tgatctgctg
     1921 acaagtgact gtcaaataca tcagtcaatg gacgattcat atcccactta atgtaatcta
     1981 agtttgattc atcaatgatt ttacacattt gatcgaaaat attttcaaca acctcatcta
     2041 acccaaaatt taatacatat tggttcctac catgattagg ttttcgatta ggtgtagaaa
     2101 taatccaatt cggatgtgtg cgatataact cactatcctt attcaccatt tcaggttcaa
     2161 accataaacc aaatttcata ccaaaactac gaattttttc tgaaaggctg ccgattcctc
     2221 taggtagtct tttaggattt acatgccaat ctcctaatcc tgcacaatcg ttttctcttt
     2281 ctccaaacca gccatcatct aatacaaata attcaatacc aacatcaaat gcttttttag
     2341 caatatttac caacttttct tcgtcaaaat caaaatatgt tgcttcccaa ttattaatta
     2401 agacaggacg gtctttttct cgccacttac cacgagctaa gcgttttcta aatagttgat
     2461 gaaaagtttg actcatggca tttaaaccct tacttgaata aaccagaatc gcttctggtg
     2521 ctgtaaatat ttctcctgga ttgagtttcc aattaaatcc aaatggattg atgccaactt
     2581 gtagtctagt aacatcccat gtatctactt ctgcttgaat taagaagttt cctgagtaaa
     2641 ctagtgctgc accaatagct tctccttgat tttcatcagt attttttctt tttaaagtaa
     2701 caaatgggtt atgctgatgg cttgaaatac ctcgagtaga ttcgattgat tgaattcctt
     2761 gttgtaaagt acgcgactta atagttcttt ctctacccca agcaccagat aattgttgcc
     2821 attcataatt tgcatcgggt aaatccaatg ataaactgct aatattctcg attatttgaa
     2881 tatcttttcc tttattcata actcgcgcgc tacgagtaat aacaggataa tctctataaa
     2941 ttgtatataa taattctact tgaacatttg taactgaatc taaaatataa actttcaatg
     3001 ttttgctttc atcattatta tcaacatagg tagcaggtaa accttctagt ttaggttttc
     3061 cttcttccac ttcgtaccct tgatagacaa aatctgataa gcgagagcca tttttctgta
     3121 gtaatgaaat agcaggatgt ctaaaatctg ttgtaccata ttcaggaaat tcttgtttta
     3181 agtgctctaa cgaatatagc aaatcgtttt catttctata ggttgtcata ggccgatgat
     3241 gcatctcaat tagatgacta tagtcattat ctggtaagtt ctcaccataa tataaatgca
     3301 ataatttacc atcttccgat atcttaaaaa tgtaacttat ctcctgatta aacaaatgaa
     3361 attgttggtt ttctgatcga taaataatat tcatttcatt atccttatac ataagctctc
     3421 ctccgtttct ttattgcatt ataaaaaacg ctcacataaa tagtaagcgt tta
//
LOCUS       DACDEA010000139         3401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDEA010000139 DACDEA010000000
VERSION     DACDEA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.143"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IV136_002703"
     CDS             complement(<1..2303)
                     /locus_tag="IV136_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466624.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IV136_002704"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IV136_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          928 a          764 c          549 g         1160 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat a
//
LOCUS       DACDEA010000140         3315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDEA010000140 DACDEA010000000
VERSION     DACDEA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.144"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..1104
                     /locus_tag="IV136_002705"
     CDS             64..1104
                     /locus_tag="IV136_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8466625.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1928..2260
                     /locus_tag="IV136_002706"
     CDS             1928..2260
                     /locus_tag="IV136_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8466626.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2879..3220
                     /locus_tag="IV136_002707"
     CDS             2879..3220
                     /locus_tag="IV136_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8466627.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1239 a          453 c          634 g          989 t
ORIGIN      
        1 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
       61 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      121 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      181 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      241 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      301 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      361 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      421 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      481 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      541 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      601 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
      661 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
      721 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
      781 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
      841 aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc
      901 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
      961 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1021 ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact
     1081 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1141 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta caaaataata
     1201 gctattttaa tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt
     1261 tacgtcataa gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt
     1321 gcatactcct tttttttgag ttataataat acgcgaagag agataatttc attatttaaa
     1381 caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt
     1441 ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt tttgcacttt
     1501 tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
     1561 ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
     1621 cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat
     1681 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
     1741 aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
     1801 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
     1861 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
     1921 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
     1981 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
     2041 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
     2101 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
     2161 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
     2221 gcgttgtacc gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt
     2281 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag
     2341 tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg
     2401 gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     2461 atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga
     2521 agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac
     2581 tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     2641 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa
     2701 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa
     2761 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag
     2821 tgaaaaaatt aatataaagc atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     2881 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     2941 ttgggattac aaaggatttg tgacgtttag aaggggcgga aattgtaaag gattagatgt
     3001 tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc caattggatt
     3061 cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga
     3121 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
     3181 ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
     3241 acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt accttaggca
     3301 ttgagtaagg aattt
//
LOCUS       DACDEA010000141         3257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDEA010000141 DACDEA010000000
VERSION     DACDEA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.145"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..310)
                     /locus_tag="IV136_002708"
     CDS             complement(65..310)
                     /locus_tag="IV136_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466628.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(304..705)
                     /locus_tag="IV136_002709"
     CDS             complement(304..705)
                     /locus_tag="IV136_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8466629.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(892..2700)
                     /gene="uidA"
                     /locus_tag="IV136_002710"
     CDS             complement(892..2700)
                     /gene="uidA"
                     /locus_tag="IV136_002710"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8466630.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2723..>3257)
                     /locus_tag="IV136_002711"
     CDS             complement(2723..>3257)
                     /locus_tag="IV136_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8466631.1"
                     /translation="RILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYI
                     SPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASK
                     GTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEF
                     KGVSPIDAYCGLKDTFI"
BASE COUNT         1020 a          639 c          411 g         1187 t
ORIGIN      
        1 tactaattcc ttaactatta gaagattctt ttaaacagaa aaagtggaaa ggggattttt
       61 ttaattagtt aattttaaat tttaagatcg cttctattaa tttaatttct agctgactta
      121 tcatatattc atctacatag gtaacgaatt gacctacctc gttaaatcca tttgtcaaac
      181 ataacttagc catataagaa tgataatgtt tgagtatcat cttgatcgaa attggatctc
      241 cttctagagc agataagata atggatagtg gaactaatag gtactgccgc tttttagatt
      301 tatctaccat aggtcatacc ccattttcaa ttgttcaact gccttctttc gttgatacca
      361 gataccactt gtgctcatat tgagaatttt accgatttca gcatcattaa aacctgcaaa
      421 ataataaagt aggataatct ttcttcttat ctccgataac tgatcaattg ctgctgataa
      481 cttttgatct gaaattaata tttccattcc aaataagaaa tagacatctt gttcatcaaa
      541 atgagtttgg gaatacctgc cttcatttga atctgccaat ggttcctcat tatcctttat
      601 gttagcgttt cgttttcgaa tagacttagc ttcgtttcgt aatacttttt tacagtaggc
      661 atcaaattgg tgttgtatag atatgtcatt gtaatgttgt tgcatgataa tatgctcctt
      721 tttccccctt tccactaata caacaatcag aaagcccctt tttttcgaaa aaactagaaa
      781 aaaaacataa aaatatttat cattctttaa acagaaaatc tttctataaa aaacaaaaaa
      841 aaacgattag gctaggctaa tcgttttttg aacattctta aaaaatccaa attatttttt
      901 ataactgaaa ttgggtattg atgaccatcg tttctgtaga tagtaagcaa tcattttagg
      961 ttttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa tcccttgact
     1021 tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattttcac atgtatctat
     1081 tacttcgtga tttgcttcgt aataagccac ttgatactct tctgtaaaca ttgttggtac
     1141 tgtatcatga aatccagcta ctgtatctgc tccatattct gtaaacatta ttggcgtatc
     1201 aggacatctt tccatccatc catctagctc ttttttcaac agatattttg catgtgccag
     1261 atcccctccc gaaacatacc aaccataata acgatttaaa cataaaacat cgacaatatc
     1321 tcctacctta caaacatctg gcgttgattt tagataagta acgattgtta caggtctttt
     1381 ctgcggatca agttctttag ttaattcaac taaaggttca aaatattctt tagcaccttc
     1441 ggaatccgaa tctggttcat ttgcaataga ccacattaca acacttgggt ggttcttgtc
     1501 gcgttctatc aattccttga tcaccgtttt atgagaattt tctgttcgta tctctttcca
     1561 cgtattatgt ttttccacat tatcatctaa taaagtggcc atgaaattta aatgtaaccc
     1621 tactgcaggt acttcatcaa taactacaat accttctcga tctgctaatc gcataatttc
     1681 ttctgagtaa ggataatgtg atgttctaaa agaattagca cctatccatt tcattaaatt
     1741 aaaatcttta atatttacag cctcattcaa accacgtcca ttaacatacg aatcctcatg
     1801 cttaccaaat cctttaaaat aaaaaggttt attattaatc aaaaatttgc cattggtaac
     1861 ctgaacactg cgaataccaa attcttctgt ataggtatcg atcactttat tatcatcaat
     1921 taaactaatt ttcatactat ataaataagc gtctagaggt tcccaaaaaa ttggattctt
     1981 aagctgaatc attcctttct ttccttcagc ttcagctaca agttgagcct cttcgtcata
     2041 tatcgctatt aagatttttc cggtccattc tgtatcgata ttgaattcta cacttgcatc
     2101 caaatcagtt attcttgtat taatagtaat atcacttata tagtctttcg gagttgtata
     2161 taacttaaca ggtctatgaa tacctgcata attgaagaaa tcaaaattaa ctaagttttc
     2221 cttttttata tccccatttt cctgtactgt ttccttcata atccccacag gtaaagtact
     2281 atcgtcaata atattgttta cagcaactgt aattcgatta ttcccgtctt ttacaaaccg
     2341 attaatttca gactcaaatg gtgtaaaccc acctttgtgt tgagtaataa actttccatt
     2401 caaaaaaact tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa
     2461 taatctttta ggaagagtca cttctttttc ataccacacc catccaacat gatttctaat
     2521 gttctctgtt tcaaataaat cattataaga agagggaact gaaatcggat aactctcttt
     2581 ttcatttatt ggttctaaag caagttcttc ctgtaagtca tcatttgtat ctaatttaaa
     2641 tctccagaga ccgcttaagt caattacttg cctcgtctct gtcataatgg gatacaacat
     2701 ataaaccact cctatctaaa tattaaataa atgtatcctt taaaccacaa taagcatcta
     2761 ttggacttac tcccttaaat tcagaaagac ccattagttt gtccaccaat atttctaaag
     2821 tttcttgttt tccatcataa gtattaatat aagtctttac ttgaggtaca tcagccaaat
     2881 gaaacggaca ctgtacagat acaaaaattg ttggaatttc atgaatgtaa aacggtatat
     2941 ccggagttcc tttacttgct ggccagacaa ttcgttgaac tgtgcctgga ttaacattag
     3001 ctatattaat gatcaagtca tattttttag ttaaattttt aattggtctc ttagcagcat
     3061 atattccagc tatattttta attttttctt cttgcgataa tcctttcaat tcttcttcag
     3121 gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa
     3181 tggcttgagg tctatttcca gcaactactt caccaaaccc accttttacc ccatgtacat
     3241 ctactagtaa aatcctt
//
LOCUS       DACDEA010000142         3134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDEA010000142 DACDEA010000000
VERSION     DACDEA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.146"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..762
                     /locus_tag="IV136_002712"
     CDS             73..762
                     /locus_tag="IV136_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8466632.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            776..2278
                     /locus_tag="IV136_002713"
     CDS             776..2278
                     /locus_tag="IV136_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466633.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2291..2767
                     /locus_tag="IV136_002714"
     CDS             2291..2767
                     /locus_tag="IV136_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8466634.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2864..2974)
                     /locus_tag="IV136_002715"
                     /pseudo
     CDS             complement(2864..2974)
                     /locus_tag="IV136_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1032 a          417 c          618 g         1067 t
ORIGIN      
        1 tatgaaagcg gatgctatat tgtttttgga gttgttatcc aaaaataata aaaaattgga
       61 gtgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg tttgattgtt
      121 tcctgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt aatgccttat
      181 tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc tgttcgagat
      241 attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa aagagattat
      301 ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt agtagaaacc
      361 ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg actatctata
      421 aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat ggcagatatt
      481 tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt aggaactact
      541 ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga tttgattaaa
      601 aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac tccagaacaa
      661 gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc tattactcga
      721 ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa ataaaatgtt
      781 taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat taccagtagc
      841 cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa atgcttatcc
      901 atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg ctggaaatgc
      961 agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac ttgcaaatgg
     1021 agataaagga acagctggtt tagctggtgg agtagcttat ctagtatata ctgcaactat
     1081 tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag gtgtagtggg
     1141 atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa aaattgaatt
     1201 accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt ctagtttagc
     1261 tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa atggtttagt
     1321 agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat atggtttttt
     1381 attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt tctggtatac
     1441 atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc aaaatatttt
     1501 ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta ctagattttt
     1561 tgcaggaaga tttgcaacaa tgatgtttgg attaccagca gcatgttatg caatgtatcg
     1621 tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg gggcccttac
     1681 atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg ttgctccttg
     1741 gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg atatcttaaa
     1801 tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat tcggtgtact
     1861 tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg catgggcatt
     1921 tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta ttcctggaat
     1981 ggaagaagaa tcagaagttc attctgaaaa agtaataaac aaaaatacat cattacatga
     2041 agagtctctt atcattattg aagctcttgg tggaaaagaa aatattgagt ctgttgaagc
     2101 ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag attattcaaa aacaaacaat
     2161 aaaagacact ggtgcaactg ctgtcttcga agtgaagggt ggcatccaag ctgtttttgg
     2221 agggaaagct gacttattaa gtcaagaaat caatcaaata ttaggaaaag ataattagga
     2281 ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa gaaacttttt atgcaccttg
     2341 ttccggaaaa atcatttcca tcacagaagt aaatgatcct gtcttttcac aaaaattact
     2401 aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt tatgctccat tggctggtcg
     2461 agtaacaaat atatttccta ctaaacatgc tattaatata aaaagcaact caggcattga
     2521 ttatttgatt catattggaa ttgatacagt tgaattacaa ggagagcctt tttcgatttg
     2581 tgttcaagaa aatgatgagg ttactcctga aacctcgttg gttgatgtgg attttgacaa
     2641 aataataaaa tctggtaaag atacttctgt tatagttgtt tttccggaga aaaatcagtt
     2701 caatgattta ataatagata atggtcgaat tgctcatggg aattcatgtg gaaaaatcac
     2761 tttttaaaaa agtgtatata aaaagctata aaataagcta aataggatat ttgtcaaatg
     2821 aagttggtga acagaaagaa ctgttttctt tctgttcatt tttttatttt attagtaatg
     2881 attccccatg ccaaatcatg atccgtaatt taaagttata aaagcttcga aatccattgg
     2941 catttcgttg gaataccttg atatgattat tcaaatataa aatatagaga gaagagagag
     3001 agggaagata tttataaagg gcaaagttat gtttactcaa cttaggaaat agaaatagat
     3061 caatttatat tctcttgtca atcgaatatg aaccttagca ctatatacca aatatagtaa
     3121 aatgctgaaa gatg
//
LOCUS       DACDEA010000143         3100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDEA010000143 DACDEA010000000
VERSION     DACDEA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.147"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..503
                     /locus_tag="IV136_002716"
     CDS             75..503
                     /locus_tag="IV136_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8466635.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IV136_002717"
     CDS             490..732
                     /locus_tag="IV136_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466636.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IV136_002718"
     CDS             complement(1032..1247)
                     /locus_tag="IV136_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466637.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IV136_002719"
     CDS             1354..1668
                     /locus_tag="IV136_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466638.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IV136_002720"
     CDS             1772..2950
                     /locus_tag="IV136_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466639.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1025 a          468 c          623 g          984 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt
//
LOCUS       DACDEA010000144         3023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDEA010000144 DACDEA010000000
VERSION     DACDEA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.148"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(241..855)
                     /locus_tag="IV136_002721"
     CDS             complement(241..855)
                     /locus_tag="IV136_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8466640.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1461..1838)
                     /locus_tag="IV136_002722"
     CDS             complement(1461..1838)
                     /locus_tag="IV136_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466641.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2138..3019)
                     /gene="galU"
                     /locus_tag="IV136_002723"
     CDS             complement(2138..3019)
                     /gene="galU"
                     /locus_tag="IV136_002723"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8466642.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          916 a          633 c          468 g         1006 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1321 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1381 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1441 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1501 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1561 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1621 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1681 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1741 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1801 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1861 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1921 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
     1981 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     2041 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2101 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2161 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2221 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2281 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2341 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2401 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2461 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2521 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2581 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2641 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2701 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2761 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2821 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2881 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     2941 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     3001 ctgctttttt gaccttcact tgc
//
LOCUS       DACDEA010000145         2932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDEA010000145 DACDEA010000000
VERSION     DACDEA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.150"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..252)
                     /locus_tag="IV136_002724"
     CDS             complement(22..252)
                     /locus_tag="IV136_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466643.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IV136_002725"
     CDS             complement(688..1254)
                     /locus_tag="IV136_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8466644.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IV136_002726"
     CDS             complement(1254..2210)
                     /locus_tag="IV136_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8466645.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IV136_002727"
     CDS             complement(2210..2866)
                     /locus_tag="IV136_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8466646.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          894 a          584 c          521 g          933 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa ga
//
LOCUS       DACDEA010000146         2921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDEA010000146 DACDEA010000000
VERSION     DACDEA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.151"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            535..>2921
                     /gene="esp"
                     /locus_tag="IV136_002728"
     CDS             535..>2921
                     /gene="esp"
                     /locus_tag="IV136_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8466647.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1058 a          447 c          578 g          838 t
ORIGIN      
        1 acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat
       61 tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt ataattataa
      121 agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact tatgtcacag
      181 tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact ctgaagttta
      241 atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc atagaaatga acttaatttc
      301 agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt aaaaagcaca
      361 aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca tgaaattctc
      421 taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa tacgtatcag
      481 atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg aataatggtt
      541 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
      601 atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct tgtcttggga
      661 acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact tgaaacagcg
      721 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
      781 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
      841 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
      901 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
      961 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
     1021 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
     1081 ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg ggtaattacg
     1141 tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct caagaccgat
     1201 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccgggt
     1261 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
     1321 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgagaaacct
     1381 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
     1441 cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg cacgccttgg
     1501 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1561 atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac aaaagtagct
     1621 gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc aataactaat
     1681 aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt cgttattcga
     1741 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     1801 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     1861 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     1921 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     1981 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     2041 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     2101 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     2161 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     2221 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     2281 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     2341 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     2401 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     2461 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     2521 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2581 ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa taagtacttc
     2641 ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc taaaggtgta
     2701 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     2761 gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa tccaggagaa
     2821 aacattcctg caggctacca caaagtaacc ttcaccgcag gggaaggaac aagtattgaa
     2881 agtggaacaa cagtctttgc agtgaaagac ggcgtaagct t
//
LOCUS       DACDEA010000147         2801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDEA010000147 DACDEA010000000
VERSION     DACDEA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.152"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..985
                     /locus_tag="IV136_002729"
     CDS             <1..985
                     /locus_tag="IV136_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8466648.1"
                     /translation="VTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQELSNH
                     TNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQNVID
                     AAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVRFGNV
                     MGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLK
                     MPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDK
                     MYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            997..2001
                     /gene="neuB"
                     /locus_tag="IV136_002730"
     CDS             997..2001
                     /gene="neuB"
                     /locus_tag="IV136_002730"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8466649.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1998..>2801
                     /gene="neuC"
                     /locus_tag="IV136_002731"
     CDS             1998..>2801
                     /gene="neuC"
                     /locus_tag="IV136_002731"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8466650.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNL"
BASE COUNT         1038 a          363 c          541 g          859 t
ORIGIN      
        1 agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt taaaatatga
       61 accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata tgcaacaaga
      121 gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact ctcaaaggat
      181 tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga aacacgttcc
      241 agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt
      301 aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta cagataaagt
      361 agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat taataatagc
      421 tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt ttggaaatgt
      481 catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa acaataaaga
      541 tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg atgctgcaag
      601 attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct taaaaatgcc
      661 ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg atacacatga
      721 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
      781 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
      841 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
      901 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
      961 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
     1021 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
     1081 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
     1141 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
     1201 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat aaaagattat
     1261 tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag tgcagacttt
     1321 ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac aaatttacct
     1381 tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg tatggctgat
     1441 attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac tattttacat
     1501 tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat taacacatta
     1561 aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata tgatgttggt
     1621 atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt ggataaggaa
     1681 ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga ttttgtaaag
     1741 catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat gccttctgaa
     1801 cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt aaatgtggga
     1861 gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat agctcctaag
     1921 tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg
     1981 cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga gctgattatg
     2041 gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta tcatttgttg
     2101 tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc ttagctgacg
     2161 gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag ggaaacatgg
     2221 ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa actagccctg
     2281 attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata gcttgtttgt
     2341 atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact gtagatgaat
     2401 caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg gaaaaaagca
     2461 aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc ggagctccta
     2521 gaatagatac tattttaaat aatagattac ctagttttag agatataaaa gaaaaatatc
     2581 atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac
     2641 tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa caatctcaac
     2701 caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga aaaatgtacc
     2761 agaaatattt aaaaacggat attatttata ttacgaattt a
//
LOCUS       DACDEA010000148         2740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDEA010000148 DACDEA010000000
VERSION     DACDEA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.153"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IV136_002732"
     CDS             complement(<1..302)
                     /locus_tag="IV136_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8466651.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="IV136_002733"
     CDS             661..915
                     /locus_tag="IV136_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8466652.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="IV136_002734"
     CDS             916..1233
                     /locus_tag="IV136_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8466653.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1792..2727
                     /locus_tag="IV136_002735"
     CDS             1792..2727
                     /locus_tag="IV136_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466654.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          968 a          390 c          510 g          872 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acatccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac tttcaaaaga
     1861 ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata caagtttaat
     1921 tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc tgagagtagg
     1981 ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat atatacgttc
     2041 cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga atatctcaag
     2101 gggaatagct atctcaagat tcgattttca catatattaa ttttactacc atatttgaat
     2161 gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa tcaaggaaat
     2221 atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg ttacggaatt
     2281 ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg ttcattatta
     2341 atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga aaacatgcgt
     2401 ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt tttgaaaatt
     2461 caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc acattttggt
     2521 gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa aatcaatgct
     2581 ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat aacagatatc
     2641 tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact tgttaataaa
     2701 aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DACDEA010000149         2602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDEA010000149 DACDEA010000000
VERSION     DACDEA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.154"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..711
                     /locus_tag="IV136_002736"
     CDS             100..711
                     /locus_tag="IV136_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8466655.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            784..1713
                     /locus_tag="IV136_002737"
     CDS             784..1713
                     /locus_tag="IV136_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8466656.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1871..>2602
                     /locus_tag="IV136_002738"
     CDS             1871..>2602
                     /locus_tag="IV136_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP8466657.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQ"
BASE COUNT          909 a          360 c          483 g          850 t
ORIGIN      
        1 tataataaaa tgtattatag gcattttttt acaagaaata gaagcttgaa gtttatatga
       61 agttataaaa tttaatttat attggaagga ggaaaataaa tgtattttat actacctaca
      121 ctttatagcc ttttatgtgt aatggcacca tgtattattt atatggtatt tttttgcaaa
      181 aaaaataccc tgaaacacaa tatcctgatt ttgattttta tgctgtactg ttggaaagtt
      241 tttgaattga ctggtacagg tggactaaca gatatttttt atagacctga tacaggtagg
      301 aaattttctc tagtacaggg cagtgttaat cttgtaccat tcagtggact aaacattagc
      361 tttatattaa atatagtaat gtgtgtgccc ttaggattct tactaccaat catatggaaa
      421 agttatagga aaatagttcc tgttttcttg gctggggctc tattttcact aattattgaa
      481 gtctcacaaa ttataactac aagagcaaca gatattgatg atttaatagc aaataccttt
      541 ggtgcggtta taggctttat actgtgggct gttttaagta gagtggtgtc cttgaaggac
      601 aagtcatcta cagtagatta tttagaacct tatatttata tattgattgg ttttaccgga
      661 aaattcttac tgtattttcc tttctggttt gcttttgaag tattatcctg ataattccat
      721 agaaaataat gagtttgagt tgagaaagaa tattcttgta cacaactctg gaaaggagga
      781 agtatgaaaa gattcttaaa atgggggctt gttcttttag cgtctttgat tgtgattcta
      841 atgtgtacag cggttgttat ttcttatgtg aatcggccgt atgcaagagc tgtttatgat
      901 aggattaaac gcattcttga caaagatttt tatgaccatc tatatctagg ttcttcattt
      961 gaaataataa accttgatac aatggatatt catagctata tgaaaaaaga ccaagaagaa
     1021 ttacccgcct cattgagcaa attatttacg attgattatg cgcttagtgt cctaagttta
     1081 gatgaacaga ttacaattag tcctactgca cttgacatgc agaaagctga ctcatctact
     1141 gcacatcttc aagaaggaga tattttatct gtttatgaca ttatctgtgc tatggtctta
     1201 ccctcaggaa atgatgcagc ttatagtttg gcaatttata caggtggaaa acttgatcct
     1261 gatgcaaaga atgatgaaga acgctatcaa agcttcatga aaaacttaga tcgttatcta
     1321 agtgagcaag gctataaaca tactcatttt actgacgctg ctggctatga ctccaatgct
     1381 acaacatcag cggaagattt gacaaaagta gctgcaaagc tactaaagaa tgataaattt
     1441 gcagaaatag ttgccaagga aacttataga gcaaaatcaa ttaagggaaa tgtttataac
     1501 tggaatacta cgaatcaatt tatgcaacaa gataatagat actttactga aggtgtaaaa
     1561 ggtgtaaaaa ctggatcgtt tgatgggaga tacaatctat gtgtgtatta tacatctagt
     1621 tctgataacc actatttact tgtaagtttt ggttcagaga gtgatagtag gcgttatgta
     1681 gatgttcaaa atctattgca taatctgcct tgaaatttcg tgcctttaag cgcgtatcta
     1741 aagtttcttt gaaaagtttg agaatgaaga aagtgagcag tcgaaaaacg gttgctcact
     1801 tttttgccct aaaatcgaaa aaatataaaa aagtccttta caacacgtct caggaaaaac
     1861 tgctaagtca attctaatat cttgtggagc aagacttgca ccttttgata taagagagct
     1921 tagagaacta atgagcgaag atgaactaga actcgataaa attggagata gaaaaactgc
     1981 cttgtttgta ataatatcag atacagatga tacatttaat tttgtagttt caataatgta
     2041 ttctcaatta tttaatctac tatgtgataa ggcagatgat gtgtatggtg gaagacttcc
     2101 agttcatgtt aggtgtctac ttgatgagtt tgcaaatatc ggtttgattc ctaaatttga
     2161 aaaactaata gcgacaattc gttcaagaga aatatcggca agtataatac tgcaagcaca
     2221 atctcaatta aaagcaattt ataaagacca tgcagataca atagttggta actgtgactc
     2281 tacacttttt ctaggaggaa aagaaaaaac aacagtaaaa gaattatcag aaaccttagg
     2341 aaaagaaacc atagacctat ataacacatc agaaacaaga tcaaatcaaa aatcttttgg
     2401 tctaaattat caaaagactg gcaaggaact tatgagtcaa gatgaaataa ctgtaatgga
     2461 tggtggtaag tgtatttacc agctaagagg agcaagacct tttctatctg ataaatttga
     2521 tattacaaag cataagaatt acaaattgtt ggaagattat gataagaaaa acttgtttga
     2581 tgtagaagag tatttaaaac aa
//
LOCUS       DACDEA010000150         2527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDEA010000150 DACDEA010000000
VERSION     DACDEA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.155"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..698
                     /locus_tag="IV136_002739"
     CDS             <1..698
                     /locus_tag="IV136_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8466658.1"
                     /translation="KNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSV
                     KYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMF
                     LRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAI
                     TNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELR
                     EKVLRSIIDKSIE"
     gene            928..1158
                     /locus_tag="IV136_002740"
     CDS             928..1158
                     /locus_tag="IV136_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8466659.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1381..1479)
                     /locus_tag="IV136_002741"
                     /pseudo
     CDS             complement(1381..1479)
                     /locus_tag="IV136_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1744..2199)
                     /gene="tnpA"
                     /locus_tag="IV136_002742"
     CDS             complement(1744..2199)
                     /gene="tnpA"
                     /locus_tag="IV136_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8466660.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          803 a          428 c          411 g          885 t
ORIGIN      
        1 agaaaaacac aaaagttggc gatgctacta ttattcgctt ttgtcaaaaa attggttttt
       61 ctggtttttc tgatttaaaa attgaaattg caaaggaaga ctttagtcaa aaaaaagaac
      121 atccgactag tgtaaagtac tatgatgaaa ttgctaatag tttaacagaa gctctctatt
      181 ccactattag gcttttagac gaagaaaaat tgaatgaagc cattaaaatg atcactcaat
      241 caaaaaacat atatatattt ggagtgggat ctagtggaaa tactagtctt gatttagaaa
      301 atatgtttct tcgcgtagga gttcaagcaa aagctgtact agaccctcat tttcaagccc
      361 aagtcgcttc tttattaaca gttaatgatt tagtaatcat tttttcttta tctgggaaaa
      421 cgaaagatac ttatgattca ttgaaaatag ctaaaaaaaa tggcgcaaaa attattgcta
      481 taactaacta tattcattca cctataggaa agtctgcaga cttagtttta caaacagcca
      541 ttgaagaatt tcttaatggt ggatctttag ctggaaaaat atcacaactt tatatttgtg
      601 atctattagt tcatggttat gaacaaaaca ataatattga ttcagttgaa ttaagagaaa
      661 aagttttgcg ctctattatt gataaaagta ttgagtgaac tacccccact tagctaaacc
      721 taacggtttt taacgattga agtaggcaat tcttgggatg agagcatact tgatagctta
      781 ttttttacta atagcggtgg ctcttattga ccaaatatcc ctgtagttcc tacggttctt
      841 gattttatct aaagatattt ttatcgtgga agctattgat caacttgaaa gaaaaacgtg
      901 tagctcatta tcactagttt atcgagtatg gatgaagcaa tggatcatcc tctcttaaat
      961 aaatttatta aggatctgat ccttcaaatt ttttccatga tcgatgagca ggaacctact
     1021 gaatctaaac gacatcgggc acaaggaatt attaagattg ctaagcgaat gttttctata
     1081 aagggtgttc taagctatac agtgttaatt caaaagattc ccaatcatct tgtttataag
     1141 aacaacgttg aagattaaaa aagtgtttca atgctaaaat tgcaaaatac ttcaatgtta
     1201 tgagacaaac tgtctataag aatcaatttc cacccatttc atcacttgtt aaaagtttat
     1261 gatccttttg atttttatat taatttatct gtatttaata cagattttat ttattagttc
     1321 atctgcctat tgtattttat tttgttgcct taccatctga agtaaatata ctcttaattg
     1381 tcaaattaag ttagacagat catcaatact tacgtaactc aaaaatactt ccaaaggtgt
     1441 tcgataatcc agtgattttc taggaagatt atttctgcgc cagcattttc taatgtttga
     1501 atttgtgttt tgtaatcttg gttgcttgtg ctaattcttg aatagccgta aattgccaaa
     1561 atttcatcac cctttctgga cataagtttc tgacaccagt taaaatcaag tctatcagta
     1621 acccatacac ccgtgaggac gggtgtattt ttaatccccc tagaaggggt cgacactcgc
     1681 tgccccttaa aaggggattt ttaaaagacc gccaactgct catcttcact cacttacttc
     1741 tctttatttt ttaaatggtt ctacgtactc tcttttactt ataatatatc gaatgcgatc
     1801 atcagcttct tgttcacgga tgtatttttc atttgtcttt tgattcaatc cgactgtact
     1861 cacatagtat ctcttcgacc aaaaggattt gtttccgtat ttatatttga gatttgcgtg
     1921 ctgctcgtgg attagtaccg cactacgacc tttcagatac cccataaaac tggatataca
     1981 tatttttggt ggtactcgaa caagcatgtg aatatgatct agcattgcat gtgcttctat
     2041 gatttccaca tctttcattt cgcacaactt cctaagtatt tttccaagat cttttcgtaa
     2101 ttgcccatag attacttttc gttgatattt tggtgtaaaa ataatatgat acttacaatt
     2161 ccaacgagta tatgctaaac ttttatcgtc atttgacatg aaaaaactcc tttcgatctg
     2221 atgagcggtt ggtagccact cactagtata tcgaaaggag tttttatcgc taaagatact
     2281 tcttccacgc gtagaaacgc gcggttttct tgtttcgcac gctttacgca caaaactgac
     2341 tgaagtcatt aagaaaataa aagggtctaa aacttttaag ttttgtaccg acccccaaaa
     2401 gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggctaag tatacttttg
     2461 aacttaaatt aaagatcgtt catgattact tggatggtaa aggcggaagt gattatctgg
     2521 caaagaa
//
LOCUS       DACDEA010000151         2516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDEA010000151 DACDEA010000000
VERSION     DACDEA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.156"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..486
                     /locus_tag="IV136_002743"
     CDS             <1..486
                     /locus_tag="IV136_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466661.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(693..1094)
                     /locus_tag="IV136_002744"
     CDS             complement(693..1094)
                     /locus_tag="IV136_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466662.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1215..1298)
                     /locus_tag="IV136_002745"
                     /pseudo
     CDS             complement(1215..1298)
                     /locus_tag="IV136_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1321..2499
                     /locus_tag="IV136_002746"
     CDS             1321..2499
                     /locus_tag="IV136_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8466663.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          798 a          507 c          506 g          705 t
ORIGIN      
        1 ttaaaggaag cgatcgatca aatcaagacg gctgtcttgt atcctcataa cggattaccg
       61 attttattga tgggtgtaag cggttcagga aaaacctttt tggcagaaaa aatttatgaa
      121 tacgccaagg aacaacaggt gatagaatca aatgctccat ttatcgccta taattgtgct
      181 caatacttta atttctcgat taacaagatc gtagaaaatt taacgatcct tgatgcaatg
      241 aaggttttga gattggtaca aaaggccgtc aaagaactcg aaaaactaat ggggatcgaa
      301 ttttcaaaca atcaattgtt cttgctttac atgcactgct gctcaatgat cgaacgtatt
      361 ttacgcaaag aaagtgtaga tgaacaagca gatatcaaag aatatattca aaaagaggga
      421 cataattatg aaatatcaaa atacccggct tacaaaacga agtttgaaag tactgtaata
      481 aagtaagatt taaataagca taaacaaggt aagaaccata ggggctgtgt caagtccaaa
      541 atcttgtgta aaaaggaaat ctcaaagtac gatgttttgc cttgttgagc ctagctacgg
      601 tccaatatgc tggtaagaag tgtcaagagc aaaaagcgaa gcgtctcttg atacttctca
      661 ccagtatatt atactattgg cggcttaatg tgctaaatct cgttacgatc aaattcaaga
      721 tattttctag gtgcagaagt ccattcgtca tccttttcca tcaataaagc accgatgata
      781 cgaataacag aatcacaatt tgggaaaatt cggacaacac gttcacgacg gcgtacttct
      841 tcatttagtc tttcaagaac attcgttgtt ctcaatcgtt gacggatatt ttctggtaaa
      901 accattactt gcatggcatc ttcgaagccc tcatccaata ttttacaggc agttgcgact
      961 ttcttgtctt ccccatattt atccataata agttgtttac gttctccagc cagctcgatg
     1021 cttgccgttt tgaagatggc tttaaagtct tctttgatat cgcttgagat tttcttggag
     1081 aatttgtcgg aaatgttgcg aaggagatgt gcttggcatc tctgctagct cgcacaaaga
     1141 aggtattctt tcacagcttt tgtgagccct atatgcgcat ccgagatgac catctttact
     1201 cctgaaagac cgcgttcttt caatccgaga gtgtaaaata ttttgtgtaa atagaaaaaa
     1261 ggaagtccct tcagtagaat agagttacca caacacattc acagaaaaga ggacttccat
     1321 atgaacgatt ttactacaga aattctaaag actctagcga ataaaggcga tttgaatgaa
     1381 ttattccgtg tccatttgga aaaagctgtc aatacgcttc tcaaaacgga gttaacggct
     1441 ttcctcgatt acgaaaagta cgatcgcatt ggttttaaca cgggtaattc tcgtaacggc
     1501 tcctatgacc gtacggtcaa gaccgagtac ggggaacttc atctccagat tccgcgcgac
     1561 cgcaacggcg agttcaagca acagactgtt cctgcttata gacggacgaa tgatacctta
     1621 gaggagaccg tcattcacct cttccgaaaa ggtattacca tgtcggaaat cgcagacttg
     1681 attgagaaaa tgtatgggca tcactacacg ccccaaacca tgtccaatat gactaaatca
     1741 tttacggaag aagtcacttc atttaaggaa cgtgagcttc atgaccgtta tgccgctatt
     1801 tatatggacg caacctatat ccctttaaag cggaaaaccg tcgccaagga agccattcat
     1861 atcgcagttg gcattcgccc ggacggttca aaggaagtgt tgagctatgc gattgcacca
     1921 actgaatcta tcacgatttg ggaagagatt ttaatagacc tccaggaacg tggcgtgaaa
     1981 aacgtcctcc ttttcatcac ggatggctta aaggggatgg caggggccgt gcagcggttc
     2041 taccccaaag cccgttttca acattgctgt gtgcacgttt cccgtaatat cagtcacaaa
     2101 gtgcgtgtcg atgatcgtaa ggaagtctgt gatgatttta aaatggtgta tcaagcgtca
     2161 tctaaaaagg cggcattgga agcacgtggt gcttttgcgg aaaaatggaa aaccagttat
     2221 ccaaaaatgg tggaatcgat cctttcgaac gattacctgc tcactttcta tgattttcca
     2281 ttagccatac gcaagactat ttactctacg aacttgattg aatcctttaa taagcaaatc
     2341 aagaaataca gccacagaaa agagcagttt caaaatgaag aatcactaga gcgtttccta
     2401 gtatccatct ttgatacata caatcaaaaa ttcttaaata gaagccataa aggcttccaa
     2461 caagtgacgg atacattagc ttcaatgttt actgagtaac ccattatttt gcagaa
//
LOCUS       DACDEA010000152         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDEA010000152 DACDEA010000000
VERSION     DACDEA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.157"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV136_002747"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV136_002747"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8466664.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="IV136_002748"
     CDS             complement(1894..2286)
                     /locus_tag="IV136_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8466665.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DACDEA010000153         2461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDEA010000153 DACDEA010000000
VERSION     DACDEA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.158"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..287
                     /locus_tag="IV136_002749"
                     /pseudo
     CDS             51..287
                     /locus_tag="IV136_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            446..1741
                     /locus_tag="IV136_002750"
     CDS             446..1741
                     /locus_tag="IV136_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8466666.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1855..2253)
                     /gene="spxA"
                     /locus_tag="IV136_002751"
     CDS             complement(1855..2253)
                     /gene="spxA"
                     /locus_tag="IV136_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8466667.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          783 a          473 c          422 g          783 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgttattaa
       61 aaaatcttcc tgaaacactt gatgcacaac ttcgcacgaa acttcaaaac cttttaacgt
      121 atgaagaagg tatttacaat gctatgattt acccttattc taatggcaaa atagaggcca
      181 agattcccca tatcaagaca ctgaaacgat tatcttatgg atttaaatca tttgagaaca
      241 tgaaaatcag gatttttttg atcaatcaat tgattcaagt gaaataacaa aaaaatctga
      301 gacagaaggt cagtccttct atcggctcta tactttttga tgttggtgaa cttcaccaac
      361 atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg
      421 ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa
      481 tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg
      541 gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg
      601 tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact
      661 gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa
      721 gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag
      781 tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat
      841 cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg
      901 cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc
      961 ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt
     1021 attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc
     1081 attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc
     1141 gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca
     1201 acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg
     1261 aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca
     1321 aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta
     1381 tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg
     1441 ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga
     1501 ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac
     1561 gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc
     1621 attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa
     1681 cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt taaccaaata
     1741 aacagaacag aaagaggttt tcttctttct gttctgtcca aattattcta agatttctct
     1801 ttaccaacat catttgacaa agagccagtc ttttccaagt ttcgtattat ccttctataa
     1861 tccagccatc aattgtgctt gacgtaattc taattgtcgt acatcgcgcg gtaggaaccg
     1921 acgaatttca tcttcattaa aaccaacttg cagacgttta tcatcaatca tgattggacg
     1981 gcgaagcaat ccaggattgt tttgcacaag ttccaataat tcttgtaacg gcagatcatc
     2041 taaatccatg ttcagttttt ggaatacttt ggaacgtgtc gagatgattt cttctgttcc
     2101 gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg ttcaatggtt ctgaaaaaat
     2161 attacgttca acaaatggaa tctgatgttc ttgcaaccac gcacgagcct ttctgcagga
     2221 agtacaactt ggggaagtat ataatgttaa caacgtgcac cactcctttt cgtgataaga
     2281 atcacttttt tgtaacagaa gatcataata atctcccatc tgtttctatt atacataatc
     2341 aacttttaaa tagctacctt tttttcataa tttttttaaa aaaatgaaag ttatctaagg
     2401 ttaatataca gtaaaaccag cattctcgct ttttttatca tttaaaaatt agagttttca
     2461 a
//
LOCUS       DACDEA010000154         2459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDEA010000154 DACDEA010000000
VERSION     DACDEA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.159"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..1150
                     /locus_tag="IV136_002752"
                     /pseudo
     CDS             6..1150
                     /locus_tag="IV136_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="IV136_002753"
     CDS             1321..1779
                     /locus_tag="IV136_002753"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8466668.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IV136_002754"
     CDS             1938..2408
                     /locus_tag="IV136_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8466669.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DACDEA010000155         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDEA010000155 DACDEA010000000
VERSION     DACDEA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.160"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IV136_002755"
     CDS             206..2068
                     /locus_tag="IV136_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466670.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IV136_002756"
     CDS             2068..>2399
                     /locus_tag="IV136_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8466671.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACDEA010000156         2358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDEA010000156 DACDEA010000000
VERSION     DACDEA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.161"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..655
                     /locus_tag="IV136_002757"
     CDS             155..655
                     /locus_tag="IV136_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8466672.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(845..1189)
                     /locus_tag="IV136_002758"
     CDS             complement(845..1189)
                     /locus_tag="IV136_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8466673.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1343..2263)
                     /locus_tag="IV136_002759"
     CDS             complement(1343..2263)
                     /locus_tag="IV136_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8466674.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT          773 a          431 c          390 g          764 t
ORIGIN      
        1 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
       61 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
      121 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
      181 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
      241 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
      301 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
      361 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
      421 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
      481 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
      541 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
      601 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
      661 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
      721 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
      781 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
      841 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
      901 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
      961 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     1021 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     1081 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     1141 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     1201 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
     1261 aagtaagatt ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt
     1321 attctaaaat aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata
     1381 cgccagttgt tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat
     1441 gagttggtaa aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat
     1501 catacatttc gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc
     1561 caccgacaga cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta
     1621 cctcttctgg cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg
     1681 catcaattac ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt
     1741 taaagaagaa gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga
     1801 ctgtttttcc agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa
     1861 agcccatttt taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat
     1921 tcatcaaaat attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac
     1981 atacaacatt cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata
     2041 caaacacagg gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc
     2101 attcatataa aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat
     2161 tggctggaat caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa
     2221 ccactatttt ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca
     2281 cctctcccct actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat
     2341 acttcgaact caaatgag
//
LOCUS       DACDEA010000157         2344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDEA010000157 DACDEA010000000
VERSION     DACDEA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.162"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..2197
                     /locus_tag="IV136_002760"
     CDS             44..2197
                     /locus_tag="IV136_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8466675.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          806 a          416 c          526 g          596 t
ORIGIN      
        1 gggtacaatg aagcatgaat gtaaataggc ggaaggatag gaaatgatga aagattttat
       61 gaaaaaaata ttaaatactt cttggttaga aaaaataaga aaaggtgcac aagaacctaa
      121 gaacaaaacg tttcgccggt cccatgttcc ttttcgctta aattttcttt ttttcattat
      181 atttacgtta tttgttgcgt tgattgctag attgggttat ttgcagatcg tgaacggcga
      241 agagatggag gcgcgagtga agtctacttc tacgattacg gtccaagaaa gtactcctcg
      301 cggaatgatc tacgactcca caggaaaagc tttagttacg aataaagcga atcaggccat
      361 cacatatacg agaggaacac aagttacggc taaagatttg cttgaaatcg caacgaagct
      421 gaactcgctg atcgatgttc cggtagatga aaatctgaca gatagagata aaaaagattt
      481 ttggctggct gatcccaaac atttgaaaga agccacaaaa agactttcgg caaaagaaaa
      541 gcaattaggg aacagcgaac aatatagtgc gatggtcaat aaagtcacag ctgatgagat
      601 taatttaaac gaggagcaat taaaaattgc taccatcttt aaacggatga atagtgcata
      661 tgaattaaac actgtctata tcaaaaactc tggtgttact gatcaagagt tagctgttgt
      721 ggcagaacgc gcttctgaac ttccaggagt ttcgactggc acagactgga cacgagaata
      781 taatgcagca agttcactga aaagtattct tggcagtgtg acgactgaaa agcaaggact
      841 tccagcagat gaagcagaga aatacctagc aaaaggttat tctcggaatg accgtgtagg
      901 acaaagttat ttggaaaaac aatatgaaga tgtcctgcaa ggaaccaaaa cccaatatga
      961 agtttccctt gataatgaag gaaatgtttc gaatcaaaaa gaaatcttct ctggggaaaa
     1021 aggttctaat ttaatgttat ctatgaatgc agaattccaa tcaaaagtag aagaaatttt
     1081 gaaaaggaat tatcaaacat tgatcaacaa cggcaaagca cagtattcgc ctggtgctta
     1141 tgcagtggct atgaatcctc agacaggaga agtgctagca atgactggtt tcagtcatga
     1201 acaaggttcc aaagaaatca ccgagaatgc tttaggcacg attactagcg cctttgatcc
     1261 agggtctgta gtcaaggctg gtactttgac agcaggctgg gcaagtaacg cgatcagcgg
     1321 aaatcaagtt ttgattgatg agccgattcg tctgcaaggt gcttcagaaa aaagctctgt
     1381 ctttaatcga agcggacaag ttgctcttga tgcagtcaaa gcatcggaat tgtcttctaa
     1441 tacgtatatg atcaaagtgg cgttaaagat gttaggacta gactatacac ctggaatggg
     1501 actacctagt ttagatgaag aagctaaagc ttatcagcaa ttgagagact ctttcaaaga
     1561 gtttggtctt ggtacaacga ctgggatcga tttgccgaat gaatctcctg ggatctcacg
     1621 ttctgttgat tatatgaaga agttcaatgc ggataatggc aaagaatggt atgctccagg
     1681 taactttacc gatttggcat ttggacagtt tgacacatat acaccaatcc aattagctca
     1741 atatgcttca acagtcgcta atggcgggaa acgagtacag ccgcacctag tcaaagcgat
     1801 ctatggcaat gacgaaaatg gaaacttggg tgaagtgaag aaagaaattg gcacaacagt
     1861 agaaaatacg gtcaatattt ctgcagaaaa tatggcgatc ctccgtgaag gattccacca
     1921 agttgtccat ggtaccgatc cttacacaac agccaaacca ttagccagtg cgaaaatgga
     1981 cctttccgca aaaacaggga ctgcagaaac ggttgcggaa ggtcatccag atattgcgac
     2041 agtcaatagt aatatcgtgg catacggacc aacagacaat ccggaaattg ccatcagtgt
     2101 tgtcttgccg aacttattag acgaacaaga ccacataaac ttgacgattg ctaaagaaat
     2161 aatggacgcg tattatgata tgttcatggc aaaataaaaa agaggtcgtg aaaaagccgc
     2221 ttagtttcga gtatgaagaa aaattttgtg aaaatagctt ctcacatttt tgcttaaatt
     2281 tgacttcata ccgagaaact ggcttttgaa caccgtttac tcggcaaaaa aggttgtgac
     2341 aaaa
//
LOCUS       DACDEA010000158         2308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDEA010000158 DACDEA010000000
VERSION     DACDEA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.163"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..468)
                     /locus_tag="IV136_002761"
     CDS             complement(247..468)
                     /locus_tag="IV136_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466676.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="IV136_002762"
     CDS             complement(566..811)
                     /locus_tag="IV136_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466677.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="IV136_002763"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="IV136_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..>2308)
                     /locus_tag="IV136_002764"
     CDS             complement(2035..>2308)
                     /locus_tag="IV136_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8466678.1"
                     /translation="WESDNNLRSYLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYI
                     LISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          781 a          403 c          318 g          806 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatatatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccat
//
LOCUS       DACDEA010000159         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDEA010000159 DACDEA010000000
VERSION     DACDEA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.164"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV136_002765"
     CDS             <1..378
                     /locus_tag="IV136_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8466679.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV136_002766"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV136_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8466680.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IV136_002767"
     CDS             1859..2026
                     /locus_tag="IV136_002767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466681.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IV136_002768"
     CDS             2080..>2232
                     /locus_tag="IV136_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8466682.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDEA010000160         2229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDEA010000160 DACDEA010000000
VERSION     DACDEA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.165"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IV136_002769"
     CDS             complement(<1..281)
                     /locus_tag="IV136_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466683.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            743..2125
                     /locus_tag="IV136_002770"
                     /pseudo
     CDS             743..2125
                     /locus_tag="IV136_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          822 a          377 c          375 g          655 t
ORIGIN      
        1 aaatgagtat agtatgaatt agtaatttgg ttttcatcta agtttagctt tgatttacta
       61 aaattgatat ctcgatacat gttcaataca tctaatacaa atcgagaatc ttctttcgga
      121 aatggctcag aaagatcact ccaaatttca tcatcataat gatactcgta accttctctc
      181 aatatttcga tttttctttc ataataagat ctgttttggt cctcaaattt agccaataac
      241 tcaaattgat ttactagtga caatctctga aaactgtcca ttaaatgttt cttcctatct
      301 tgttcattaa caaaaaatta taagccctta caacgaatat tatatattaa atttaattgt
      361 aggtgaacac gaattttata aatatttatg gtgttaatga caaatgatat ccgactcctc
      421 ttcatacgtt ctggactatt tgctaataga ttaaaatttc agcatctatt tctgaccata
      481 aataaatgag ctgccgaaaa gctgccaaat cagacttgaa aataaaggat caaaaacttt
      541 taagtttctg accccgtaac tcacacttca aaatcttact taaaaaggag tctgaaagaa
      601 aaaaagagta ttcaaaattt ttttctaact agctacatca catctatgca ttgacactca
      661 aagaaccaga ataagtcatc agaaagcaag ggggtgcgga cataatcctg gacgaaaaaa
      721 taaacttagg aggaatgtcc gtgtgttttc ccacaaagag cgtgtaaggg caattcagtt
      781 atttttaaaa tacgattgct cttatgctgc aaccattcga gaattagggt atccgtcgat
      841 tggtgcacta cggaaatggt ataaagagta tttggaatcc ggagagctcc atcaggagca
      901 ccgaaaaaaa tcaaagtatt ctgaagaaca aaaacgaata gcggtgaatc attattttga
      961 atatggtcaa tgttatgcac gaaccattcg catgcttggt tatcctaata gagagtcctt
     1021 gcggcagcgg tgtgaagaac ttgcaccagg agctagaaaa ctccgaaaaa gtgcggtaaa
     1081 gttaacacaa gatcaaaaag aagctgtgtt gaaaaaattc tataaaccgc aaactaatag
     1141 gaaaaattta gccgaatctg gaggaattag tagagttact ctatatcaat ggaaaaacac
     1201 ggttcttgga aaggattttc cattgtgtat gactttaaaa aatcaggaaa aacaaaaaga
     1261 gctactttta aaggaagtag aagacttaca aaaacaagta caccaattac agctcgaaaa
     1321 agcattactc gaaggagcgg ctgaactgct aaaaaaagaa aaaggcgtca atcttctttg
     1381 tctatcaaat caagaaaaaa cgatattgat tgatgcctta cgaaatcagt tcaccttgaa
     1441 agagcttctt caacagctcc agtttcctaa aagcagctat ttttatcaaa aacaagcact
     1501 tgagaaacca gataagtatt acaaagaacg tcaaattaat tatcacgatt ttcaatcata
     1561 acttctgtgc gtatggttac cgtagaattc accaagcttt aaaaaatatg ggtaaaaagc
     1621 tttctgaaaa agttgtacgt agattgatga ctgaggaaaa tctatttgtt aaattttcta
     1681 gacgaaaaaa gtattcttcg tatgctgggg aaatttctcc tgcacacccc aatttattaa
     1741 atcggaattt taaagcaaag aagccaaatg aaaaatggtt aacagatatt actgaattta
     1801 gaattctcgc agggaaaatc tacttatcac cacttgtcga ttgttacgat ggtgctattg
     1861 ttagttggac tattggaaca aagccaactt gcttcactct atgtctaaaa agagttattc
     1921 accagataat tcagcgtgcg aaggtttctt tggacgatta aaaaacgaga tgttttatca
     1981 acgtgactgg aagaatacaa caatcaatca gtttataaac aaattggatg attatattca
     2041 ttggtataat aatcaacgaa ttaagttatc tttaggaagg ctaagccctc ttcaatatcg
     2101 gaagaagcaa agatatattt gttaaaaagg tccaaaaaaa atatccgcac cccctttttt
     2161 ttgtaaaaaa ctattgcaca atacaagtag cctacggcta tcgaaatttc cataactttc
     2221 gagcaagaa
//
LOCUS       DACDEA010000161         2216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDEA010000161 DACDEA010000000
VERSION     DACDEA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.166"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV136_002771"
     CDS             131..505
                     /locus_tag="IV136_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8466684.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV136_002772"
     CDS             complement(619..1248)
                     /locus_tag="IV136_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8466685.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV136_002773"
     tRNA            complement(1382..1454)
                     /locus_tag="IV136_002773"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IV136_002774"
     tRNA            1668..1741
                     /locus_tag="IV136_002774"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IV136_002775"
     CDS             1696..1845
                     /locus_tag="IV136_002775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466686.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
BASE COUNT          748 a          352 c          367 g          749 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acattt
//
LOCUS       DACDEA010000162         2213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDEA010000162 DACDEA010000000
VERSION     DACDEA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.167"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..265
                     /locus_tag="IV136_002776"
     CDS             <1..265
                     /locus_tag="IV136_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466687.1"
                     /translation="DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMW
                     FEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            590..1207
                     /locus_tag="IV136_002777"
     CDS             590..1207
                     /locus_tag="IV136_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466688.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1248..1547
                     /locus_tag="IV136_002778"
     CDS             1248..1547
                     /locus_tag="IV136_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466689.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1581..1748
                     /locus_tag="IV136_002779"
     CDS             1581..1748
                     /locus_tag="IV136_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8466690.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1793..>2213)
                     /locus_tag="IV136_002780"
     CDS             complement(1793..>2213)
                     /locus_tag="IV136_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466691.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          773 a          341 c          422 g          677 t
ORIGIN      
        1 agattacttt gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag
       61 agtaccacct tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc
      121 aaatgaaatg tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga
      181 atacaattat attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga
      241 aatatatagt gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca
      301 aattctgatg tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta
      361 gtttatttaa tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata
      421 taatcatgcg ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat
      481 tttctttgtt tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta
      541 atctgattgt tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt
      601 tttttatatc cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa
      661 ggaattagga atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa
      721 acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca
      781 atcattagat agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac
      841 taaggaaaag cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta
      901 caagcaacta gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta
      961 tatggccgaa gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc
     1021 gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc
     1081 gatctattct gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg
     1141 catgaaacga aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt
     1201 ttcttgatag atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga
     1261 atcaaagggt cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt
     1321 tggttcactc tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg
     1381 tttgtggtca gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa
     1441 ccgcgcggga cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt
     1501 atgcgttaat tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat
     1561 ttacaaggag gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa
     1621 ctagttttaa tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa
     1681 gtgtttatca aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg
     1741 gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat
     1801 cattcgcttt ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct
     1861 ctgcaactgg aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt
     1921 gcggatctgt tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt
     1981 gagccaataa tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa
     2041 tataacttag cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag
     2101 tattaaaatc taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa
     2161 aagcaactgt attttttata tcttcataat ctcttcctaa acgatctagt gat
//
LOCUS       DACDEA010000163         2185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDEA010000163 DACDEA010000000
VERSION     DACDEA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.168"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..157
                     /locus_tag="IV136_002781"
     CDS             <1..157
                     /locus_tag="IV136_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8466692.1"
                     /translation="NKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKW
                     IQKQIAY"
     gene            130..807
                     /locus_tag="IV136_002782"
     CDS             130..807
                     /locus_tag="IV136_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8466693.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            797..1939
                     /locus_tag="IV136_002783"
     CDS             797..1939
                     /locus_tag="IV136_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8466694.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1952..>2185
                     /locus_tag="IV136_002784"
     CDS             1952..>2185
                     /locus_tag="IV136_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8466695.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFII"
BASE COUNT          795 a          286 c          445 g          659 t
ORIGIN      
        1 gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg
       61 tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga
      121 agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt
      181 attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag
      241 ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg
      301 aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta
      361 gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag
      421 caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat
      481 ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct
      541 ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta
      601 tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt
      661 ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc
      721 actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag
      781 aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata tgggaggaac
      841 agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa
      901 tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc
      961 gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa
     1021 cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca
     1081 aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc
     1141 actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt
     1201 agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt
     1261 taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc
     1321 gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac
     1381 ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt
     1441 agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta
     1501 ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag
     1561 agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag
     1621 aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac
     1681 gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa
     1741 aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt
     1801 aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt
     1861 atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt
     1921 taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc
     1981 catggggaaa agaagactta gactcattca agaacacttt aatgatattt tactatgtgg
     2041 tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta
     2101 taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc
     2161 ccctataacc catgaattta ttata
//
LOCUS       DACDEA010000164         2162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDEA010000164 DACDEA010000000
VERSION     DACDEA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.169"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IV136_002785"
     CDS             <1..370
                     /locus_tag="IV136_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8466696.1"
                     /translation="LFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYF
                     GRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTG
                     KENWNDLYHANEVLKIAEEK"
     gene            371..1123
                     /locus_tag="IV136_002786"
     CDS             371..1123
                     /locus_tag="IV136_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8466697.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1128..2075
                     /locus_tag="IV136_002787"
     CDS             1128..2075
                     /locus_tag="IV136_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8466698.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          765 a          284 c          418 g          695 t
ORIGIN      
        1 cctatttggt ttccctaaat cagtaaaaaa acagtttggt aaatattcga atcttgaaat
       61 taacagtaat gatttagcaa catatatatt gagatacgat gataaaatta tagaaattca
      121 tttagattat tttggaagag ttcctacaag aaaatttcaa ttgtttactt tagatgaatg
      181 tatcaatgtt gacttattaa ataataaaat aagttttgaa gaaaacattg ttaaagaaat
      241 gcctgaatct gctaatgata tgtatgagaa ggagttgaaa tatttctttg aaaacgtagt
      301 aacaggcaaa gaaaactgga atgatttata tcatgcaaac gaagtattga aaatagctga
      361 ggagaaataa atggaaattt tatttactat ttgtggtaga gctgggtcta aggggtttaa
      421 aaataaaaat ttgaaatatt tggcaggaaa gcccctagtc catttcgctt tagcaatgat
      481 agatatctat caacaaaaac atcctgaaca taattgcacg acttgtttga atacggacag
      541 taatgagtta gtagaattga cgactaagtt aaatccgtct gtgaaaattg tagaacgtaa
      601 acctgaatta gctacagata ctgtagggaa aattgaagtg atacgtgatt cattcttaca
      661 actagaaaga gaagataata tatttgattt aattatagat ttagatttga cttcaccttt
      721 acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt tagataaaga
      781 cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg taaaaagaat
      841 tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac agacacctga
      901 agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa aaaaaagtaa
      961 ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa ttttagatat
     1021 tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt tagaagattc
     1081 ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg agctgttatc
     1141 ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta ggagaagaac
     1201 atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt gatatgacga
     1261 aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa gagatcatga
     1321 aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg gcagattcag
     1381 ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg attttagaat
     1441 tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca gcggctgttt
     1501 atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt
     1561 atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat ttatatgatg
     1621 tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat ccgaattctc
     1681 catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc gtaggagaag
     1741 caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt tatatagatg
     1801 atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg aaacaattca
     1861 atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc aatgaaattc
     1921 tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc catgactctc
     1981 tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt attttaagtg
     2041 gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa gattagaggg
     2101 taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa tctgggatag
     2161 aa
//
LOCUS       DACDEA010000165         2082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDEA010000165 DACDEA010000000
VERSION     DACDEA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.170"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1343
                     /locus_tag="IV136_002788"
     rRNA            <1..1343
                     /locus_tag="IV136_002788"
                     /product="23S ribosomal RNA"
     gene            1437..1552
                     /gene="rrf"
                     /locus_tag="IV136_002789"
     rRNA            1437..1552
                     /gene="rrf"
                     /locus_tag="IV136_002789"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1566..1638
                     /locus_tag="IV136_002790"
     tRNA            1566..1638
                     /locus_tag="IV136_002790"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1599..1601,aa:Val,seq:tac)
     gene            1642..1714
                     /locus_tag="IV136_002791"
     tRNA            1642..1714
                     /locus_tag="IV136_002791"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1675..1677,aa:Lys,seq:ttt)
     gene            1722..1803
                     /locus_tag="IV136_002792"
     tRNA            1722..1803
                     /locus_tag="IV136_002792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Leu,seq:tag)
     gene            1821..1893
                     /locus_tag="IV136_002793"
     tRNA            1821..1893
                     /locus_tag="IV136_002793"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1854..1856,aa:Thr,seq:tgt)
     gene            1905..1976
                     /locus_tag="IV136_002794"
     tRNA            1905..1976
                     /locus_tag="IV136_002794"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1937..1939,aa:Gly,seq:gcc)
     gene            1993..2078
                     /locus_tag="IV136_002795"
     tRNA            1993..2078
                     /locus_tag="IV136_002795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Leu,seq:taa)
BASE COUNT          546 a          463 c          609 g          464 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tgagagtagg acgtcgccac gcttaagtta
     1561 tccatggagg tttagctcag ctgggagagc atctgcctta caagcagagg gtcagcggtt
     1621 cgatcccgtt aacctccata tgagccgtta gctcagttgg tagagcatct gacttttaat
     1681 cagagggtca caggttcgag ccctgtacgg ctcatatttt tgcgggtgtg gcggaattgg
     1741 cagacgcact agatttagga tctagcgcct tacggcgtgg gggttcaagt cccttcaccc
     1801 gcataattta agttttacat gccggcttag ctcagttggt agagcatctg atttgtaatc
     1861 agagggtcga gggttcaagt cctttagccg gcatctttaa acacgcggaa atagctcagt
     1921 ggtagagcac caccttgcca aggtgggggt cgcgggttcg aaccccgttt tccgctttgc
     1981 caaagcaatc gcgccggggt ggcggaactg gcagacgcac aggacttaaa atcctgcggt
     2041 gagtgatcac cgtaccggtt cgattccggt cctcggcatt tc
//
LOCUS       DACDEA010000166         2064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDEA010000166 DACDEA010000000
VERSION     DACDEA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.171"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IV136_002796"
     CDS             complement(51..1598)
                     /locus_tag="IV136_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8466699.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IV136_002797"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IV136_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8466700.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          581 a          431 c          371 g          681 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagt
//
LOCUS       DACDEA010000167         2061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDEA010000167 DACDEA010000000
VERSION     DACDEA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.172"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..521)
                     /locus_tag="IV136_002798"
     CDS             complement(<1..521)
                     /locus_tag="IV136_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8466701.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSS"
     gene            complement(523..1191)
                     /locus_tag="IV136_002799"
     CDS             complement(523..1191)
                     /locus_tag="IV136_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8466702.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1321..1905)
                     /locus_tag="IV136_002800"
     CDS             complement(1321..1905)
                     /locus_tag="IV136_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8466703.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          725 a          346 c          278 g          712 t
ORIGIN      
        1 tttgaagacg gttcatccaa tataattaat ttagagttta gcgataaact acatccaata
       61 gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa
      121 agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca
      181 aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta
      241 ggatcttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc
      301 gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt
      361 ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta
      421 agactgcctt tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta
      481 tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa
      541 gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata
      601 gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc
      661 ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag
      721 aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat
      781 ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt
      841 cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga cactaaccaa
      901 caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg atgtcgcaaa
      961 aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac
     1021 tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac
     1081 tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac
     1141 cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc
     1201 tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata
     1261 acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttaa
     1321 ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag
     1381 catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga
     1441 gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat
     1501 agtctttaac ttatctagac aacagactgt ctttttcgtc acaaatgatg taattttttc
     1561 aacaatatct tgatatttgg ttccagctga ttggaataaa atcaaatcga atatacgttt
     1621 attctcaaaa atataattaa taaatggatc taattcattg aagttctctc tccaactttt
     1681 ctctatatct atattttcta agtttaagag gtatttggta aactcttcat tatacatatt
     1741 aataagcgca ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa
     1801 atgtttataa aaagcacccg tagttacgcc tgcggtcagg cacagttgcc ttatcttagt
     1861 ttgctgaaat ccttcttttt caaaatggct tagtccactt tctattaact tttggtgagt
     1921 tataatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat taactttatt
     1981 atcttacaca ggacttgaag tttcaatcgt taacgtttat tactgaactc attccttaga
     2041 gctaaactaa tgagaaagag c
//
LOCUS       DACDEA010000168         2008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDEA010000168 DACDEA010000000
VERSION     DACDEA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.173"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..418
                     /locus_tag="IV136_002801"
     CDS             89..418
                     /locus_tag="IV136_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8466704.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            408..695
                     /locus_tag="IV136_002802"
     CDS             408..695
                     /locus_tag="IV136_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8466705.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            979..1836
                     /locus_tag="IV136_002803"
     CDS             979..1836
                     /locus_tag="IV136_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8466706.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          747 a          268 c          396 g          597 t
ORIGIN      
        1 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
       61 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      121 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      181 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      241 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
      301 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
      361 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
      421 tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag ccattgaaag
      481 aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag ggtgaacaaa
      541 aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc acaacatata
      601 gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt ggaacgcatg
      661 aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt cttaagaagg
      721 acggcttctt tttatcgaaa atctgattcg tctacatgac taaagtcgcg agtattctct
      781 gcttctcgat aaattaaata acgtcattac tttgaggaaa gctataaatc aatatatttc
      841 agcttttcct tatttatgta ctacctttta cttattaatt ataaggtagt acatatatat
      901 ctttttttgg tagtaactaa aaataaatat gttataatag tatcaaatga atattgctaa
      961 aagaaaagag gtaggaacgt ggtagcgaag aaatatgtaa tcggtaattt taaaggtggc
     1021 gtaggtaaaa gtacgtgcgc gcaaatgtta gggtttgaat cagcagttaa tgaaggaaaa
     1081 agaaccttga taattgactt ggatatgcaa ggaaatactt ctgatgtgat gaacttaacg
     1141 catatgaact tcactaacga agaaggaggc ggagaaggtg aaccgattgt ttacgaaaat
     1201 actgtgactg acgtgttgat aaacaatctt ccacctaaag aggctgtcta taaagtaatc
     1261 aataacctct atatacttcc agcagacatg tcctttgaaa tgtatgatga ttggatcaaa
     1321 gaccaatata atgattcatt atcccaattc aaatatatga aagaaaagct agaaccatta
     1381 tttgatgagt ttgacgtcat ctatttagat gtcccacctt ctatttctgt atatagtaaa
     1441 tccgcgatgt acattgcgga ttgggcaatc gtcgtcttac agacccaagt aaaatcgatg
     1501 agaaatgcga tgcaatatct tgagtatatg gacttttttg tgaaagaatt tgatacaaat
     1561 ttatatgttg ctggtgttat tcctttcatg ttggaaagcg gtgacgcagt agatcaggaa
     1621 atgtataaac aagcccaaga gatttatggg gaacatttga ttaaaaatgt tgtactcaaa
     1681 aatgcgcgct taaaacgata cgacggatca ggtattacga tggagaagac aaagaaaggt
     1741 ctcttaaaac aatgggatcg caaaagccac gaattattta tcagtatttt gaaagaattg
     1801 gaagaacacg agaattggta tgcggaggtg gagtaattgg ctgacatcaa aggacttgat
     1861 atttttggga acaacaaagg actcgccaaa ataaaaaaag aagcagaagc aaagaaaaag
     1921 cagcaagcaa ttacaccatc tgaaaataag caattagaag tgcaccaagt tcaagaaata
     1981 aaaacttcct ccaatgttga aagacaga
//
LOCUS       DACDEA010000169         1970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDEA010000169 DACDEA010000000
VERSION     DACDEA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.174"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IV136_002804"
     CDS             5..1087
                     /locus_tag="IV136_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466707.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1970)
                     /locus_tag="IV136_002805"
     CDS             complement(1305..>1970)
                     /locus_tag="IV136_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466708.1"
                     /translation="EIIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEE
                     FYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSS
                     IRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVK
                     PIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITR
                     FDI"
BASE COUNT          672 a          352 c          340 g          606 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc
//
LOCUS       DACDEA010000170         1969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDEA010000170 DACDEA010000000
VERSION     DACDEA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.175"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..646)
                     /locus_tag="IV136_002806"
     CDS             complement(<1..646)
                     /locus_tag="IV136_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8466709.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGS"
     gene            1338..1496
                     /locus_tag="IV136_002807"
     CDS             1338..1496
                     /locus_tag="IV136_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466710.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            1568..>1969
                     /locus_tag="IV136_002808"
     CDS             1568..>1969
                     /locus_tag="IV136_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466711.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDE"
BASE COUNT          710 a          312 c          286 g          661 t
ORIGIN      
        1 cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta
       61 ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta
      121 acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa
      181 atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc
      241 cagataattc tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct
      301 ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg
      361 ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt
      421 gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct
      481 ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat
      541 ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg
      601 ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact ttctccttct
      661 aaatgataat gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa
      721 attttatagt ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac
      781 tacgcgtata gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca
      841 tgctcctaaa actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt
      901 gaataagttg ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat
      961 atacaaatat gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac
     1021 atgtttatcg ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa
     1081 tacaattttt gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct
     1141 gattagcgac aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa
     1201 tagcagcaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
     1261 tatgtgctta cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta
     1321 tagaatccag agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata
     1381 atactctaga taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag
     1441 ttcgctttga cgtagaagat attaaagact ttatgaatac atttaaaata acttgaaatt
     1501 ttcttctcgc cgcatatact aaaattagtg aaatatactg ccttactttt tttaaggagt
     1561 gaggattttg cctacaaagc tttcaaatgg gaaatataaa actaatctga gatatccaaa
     1621 aaggtttaaa gaaataacag gaattgcatc agaaaagtat caaaaaacat ttccaaatag
     1681 gcaattggcg attaaagccg aaaatgatat gaagaagaaa atagaaaaag ttttgagaga
     1741 ggagaatgca aactcattag aattaaaagg aaaaataacg tttaaaaaat tttacgaatc
     1801 taaatggctt cctagatatg aattaggtca aacaattaga tcaaacaggc ctcctagtga
     1861 tataaccatt agtaatacaa aagatatatt tcggttgcat attttaccta tgtttggtga
     1921 atacgcaatg aattatctaa atatcaatac tgagataata tcagatgaa
//
LOCUS       DACDEA010000171         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDEA010000171 DACDEA010000000
VERSION     DACDEA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.176"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..596
                     /locus_tag="IV136_002809"
     CDS             <1..596
                     /locus_tag="IV136_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8466712.1"
                     /translation="KQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPI
                     YGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATD
                     WHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEE
                     SQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
     gene            796..999
                     /locus_tag="IV136_002810"
     CDS             796..999
                     /locus_tag="IV136_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466713.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1559..>1932
                     /locus_tag="IV136_002811"
     CDS             1559..>1932
                     /locus_tag="IV136_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8466714.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSM"
BASE COUNT          627 a          293 c          455 g          557 t
ORIGIN      
        1 tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca gatattgctt
       61 gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt gaccctgtgt
      121 cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg ggtagtcgtg
      181 ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacga aaaaaacaga
      241 ttgtaccaaa agaaatcgaa acatggtggc gtatagaaat gcagctaaga cgtggcaaag
      301 caacagattg gcatgcgatg gttcgggaaa gtttagatag ctttgcaagt cctcattact
      361 tgcctggaga tgtaaaacct gttgatagaa ttatgataaa agggctgaat caagatcatt
      421 cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg ttgaaagaag
      481 aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa tctgcaaacg
      541 acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag aagtgatacc
      601 gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg acgtgagttt
      661 acgataagcg attattttgc gtgaagcaaa ataaagcgta aaaggagttg agagcgtgac
      721 acgctttcgg ctcctttttt cgttgtcact tgtgacagcg aataccctcg gagagcattt
      781 ggaattgtga cagccatgtc acaattagga aaatgttact ctttgtcaaa gagtaacaga
      841 aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggttcacctt agggtttagt
      901 atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact
      961 gatagccaaa aaatcaatgg tttgtctggt agaaaataat tagcagattt acaagtggga
     1021 aataaaaagc tgaaagaaga aaattgcatt tcaaactaca ttttctacaa tagctctaaa
     1081 atggcatatt ttgcgttctg agcggtttta gcgtttattt cgtttagtta tcggcacaat
     1141 cggtgaaaca cgttagagga gcgaaaatgg agcctgtaag gcgactggta gcggaacggt
     1201 cgtgttagat tgtgtagccg atagctgaac gaaataaatg ctggcagcgc cattctattt
     1261 cggcaggagg aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttatttttt
     1321 ctagaggagt gctataatca aagaaaatga gaaaaaagag taaaaaaata gtcgagcagt
     1381 gttgacgcac tctcgacctt tacggaaact acttcaccta gttccgtaac tcaagttcat
     1441 tatacgaata tttttaggtt tcttcaagct agagattttt ggatgtggat ttttagagtt
     1501 acgtgaactc tggaaattct ttccaagaat ctcttttttc gttgaggagg agctattttt
     1561 gagtaaaaaa ggcataatcg taaatgagtg ggaaaaactt aaccgtattt ctaacaggcg
     1621 gttaagtgtt gacgataagg tacaccccct aaaattaagt tggtcaatcg atcgaattac
     1681 aatcgtcgga aaactgaatg ataccatagc ttattggacg caaaatggag ttgtgtttgt
     1741 cgattttgag caattaatgc gtttgaatga aaatcaaggg tatttgaagt cggttggtac
     1801 aaatggttgg caattatttg accaatatgg agaagatatc gcctatatcg aaatgttaaa
     1861 atttcaccct gacaaaggtc gtattgattt taatccaaat aaaattggcg agtttttaaa
     1921 ggggtcgatg aa
//
LOCUS       DACDEA010000172         1913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDEA010000172 DACDEA010000000
VERSION     DACDEA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.177"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(460..1779)
                     /locus_tag="IV136_002812"
     CDS             complement(460..1779)
                     /locus_tag="IV136_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8466715.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          584 a          353 c          376 g          600 t
ORIGIN      
        1 gcaaaacatt tttaacgtaa gcgccccata gaacagtacc ttactaacaa atgcacctca
       61 agatatactt gagctatcaa gaagtctgga ggtcttctat gtataaatta aatgagattg
      121 ttcctgtcca attaggaaac gaaaagagtg caccagaaaa aggtaaaccc gcggctgcca
      181 agttagcgat gcgcatcaaa agagagaatg gttctgaagt tttgatctat aacggtatta
      241 acaattatat tcttcacctg aataattcat agctttaatt ctttggcaca ttttattgaa
      301 atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca
      361 ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc
      421 tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg
      481 cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt
      541 gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg
      601 aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt
      661 gactaagttg taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg
      721 actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt
      781 gccacgtttg gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt
      841 cacgataaat tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac
      901 gcgacgactt tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg
      961 ttcttcccac ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct
     1021 acgattattc ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac
     1081 accaggtgta gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg
     1141 attatagtgc tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt
     1201 tcctgaacgt agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg
     1261 atggtaacca taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt
     1321 atctgaatgg gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc
     1381 tttatcgata agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg
     1441 atctaaaaat cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt
     1501 aaacacagga tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa
     1561 ttgcataatg aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc
     1621 ttcaatgtga atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt
     1681 cactaggacg agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt
     1741 tgaattgaat tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg
     1801 gttggtttag tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg
     1861 aagatgaaaa agtaattgta agctgccgtg aggcagtttc acatggtttt aat
//
LOCUS       DACDEA010000173         1857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDEA010000173 DACDEA010000000
VERSION     DACDEA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.178"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IV136_002813"
     CDS             273..1751
                     /locus_tag="IV136_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8466716.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          679 a          257 c          328 g          593 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaa
//
LOCUS       DACDEA010000174         1780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDEA010000174 DACDEA010000000
VERSION     DACDEA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.180"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..559
                     /locus_tag="IV136_002814"
     CDS             203..559
                     /locus_tag="IV136_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8466717.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            569..1468
                     /locus_tag="IV136_002815"
     CDS             569..1468
                     /locus_tag="IV136_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8466718.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          609 a          300 c          360 g          511 t
ORIGIN      
        1 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
       61 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
      121 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
      181 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
      241 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
      301 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
      361 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
      421 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
      481 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
      541 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
      601 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
      661 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
      721 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
      781 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
      841 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
      901 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
      961 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
     1021 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
     1081 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
     1141 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
     1201 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
     1261 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
     1321 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
     1381 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
     1441 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
     1501 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
     1561 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
     1621 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
     1681 tttttattta attataatta atattgattg aagtctaggc agtcaagtcg agagtgtaaa
     1741 atattttgtg taaatgaaaa aatccataca aaaaaggaag
//
LOCUS       DACDEA010000175         1694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDEA010000175 DACDEA010000000
VERSION     DACDEA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.181"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV136_002816"
     CDS             <1..1350
                     /locus_tag="IV136_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8466719.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>1694
                     /locus_tag="IV136_002817"
     CDS             1484..>1694
                     /locus_tag="IV136_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8466720.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSE"
BASE COUNT          670 a          251 c          357 g          416 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gaga
//
LOCUS       DACDEA010000176         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDEA010000176 DACDEA010000000
VERSION     DACDEA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.182"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..416
                     /locus_tag="IV136_002818"
     CDS             <1..416
                     /locus_tag="IV136_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8466721.1"
                     /translation="VLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTL
                     VAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFN
                     IAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
     gene            413..1363
                     /locus_tag="IV136_002819"
     CDS             413..1363
                     /locus_tag="IV136_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8466722.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1378..1611
                     /locus_tag="IV136_002820"
     CDS             1378..1611
                     /locus_tag="IV136_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8466723.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
BASE COUNT          621 a          202 c          308 g          503 t
ORIGIN      
        1 cggttttagt gttggttata gtagttctat tttttaaaga attattaatc acttcttttg
       61 atccattaat ggcaaaagca atagggatga atgtcagttt ttatcattat ctattgatgg
      121 ttctattaac tttagtagca gtaacagcta tgcaaagtgt gggaactata ttaattgttg
      181 caatgttaat tacaccagca gctacagcat atttatatgc aaatagcttg aaaactatga
      241 tatttatttc tgcaacagtc ggagcaagtt catcattgct tggattgttt attggttata
      301 gttttaacat agcagcagga tcaagtattg ttttaacagc ggcattctta tttgtaatta
      361 gttttctaat ctcaccaaaa caaaaatttt taagaaagaa ggagagatcg ttatgatgag
      421 aaaatggaaa gcagtactgg gaagtctggg gattttgatt gctcttttta tattcggggc
      481 atgttcaata aatagtaaag acaaagataa agtggcttcg aacgaaaaat taaaagtagt
      541 agttactaat tcgattttag cagatattac cgaaaatata gcaaaagata aaattgattt
      601 acacagtatc gtacctattg ggaaagatcc ccacgaatat gaacctttgc ctgaagatgt
      661 tcaaaaaact tcaaaagcag atttgatttt ttataacggt gttaacttgg aaactggagg
      721 aaatgcttgg tttacaaaat tagttaaaaa tgcgaacaaa gaggaaaaca aagactattt
      781 tgcagcaagt gatggcatag atgttattta cttagagggt cagagtgaga aagggaagga
      841 agatcctcat gcttggttaa atttagaaaa cggtattatt tacgctaaaa atattgaaaa
      901 acagttagcg gaaaaagatc ctgataataa aaaattctat aaagaaaatc tagataagta
      961 tattgaaaaa ttggattctc tagacaaaga agctaaatct aaatttgctt caattccgaa
     1021 tgataaaaaa atgattgtta caagtgaagg atgctttaaa tatttctcga aagcgtataa
     1081 tgtgccttct gcttacattt gggaaatcaa cactgaagaa gaaggaacac cagatcaaat
     1141 aaaacactta gttgaaaaat tacgcacaac aaaagttccc tccttattcg tagaaagcag
     1201 tgtggatgat agaccaatga aaacagtatc aaaagatacc aatattccta tctattcaac
     1261 gatttttact gattcaattg cagaaaaagg acaagatggt gatagttact atgcgatgat
     1321 gaaatggaac ctggataaaa ttgctgaagg cctttcgaaa taatttggag ggtaggtatg
     1381 gtacaaatta aaacgactac tatactaatt tatttactag caattcagga attatcaaaa
     1441 agaaaaaaga aattaaaaaa taatgattta gcaaaagttc taagtgttag tccagcatct
     1501 gtaagtgaga tgctggctaa acttagatat gaggactatt tagatatagg atttcaatta
     1561 actgataaag ggaaaaaatt tattgacgat tacaaaaaag gttttaagta aaagacttta
     1621 tttttaggga ggat
//
LOCUS       DACDEA010000177         1632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDEA010000177 DACDEA010000000
VERSION     DACDEA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.183"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IV136_002821"
     CDS             <1..355
                     /locus_tag="IV136_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466724.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..985
                     /locus_tag="IV136_002822"
                     /pseudo
     CDS             615..985
                     /locus_tag="IV136_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1632
                     /locus_tag="IV136_002823"
     CDS             985..>1632
                     /locus_tag="IV136_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8466725.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          600 a          233 c          336 g          463 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggtacaa
     1141 gagtacagca aagtcctcta ttatctttgg aagaagaaaa atagacaatc cttctattca
     1201 tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt ggcattatct ttatcgtgca
     1261 attgatgcgg acggcttaac cttagatatc tggttacgaa agaaacggga tacgcaagca
     1321 gcctatgctt tcttaaaacg actccataaa cagtttggtg agccgaaagc aattgtgacc
     1381 gataaagcac cttctcttgg ctccgccttt agaaagttac agagtgtggg tttatatact
     1441 aagacagagc accgaactgt gaagtatctt aacaatttaa tagaacaaga ccatcgacct
     1501 attaaacgac gaaataaatt ttatcaaagt ctccgtacag cctcttccac gattaagggc
     1561 atggagacca ttcgaggaat atataaaaag aaccgaagaa atggaacgct cttcggcttt
     1621 tcggtgtcta ct
//
LOCUS       DACDEA010000178         1613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDEA010000178 DACDEA010000000
VERSION     DACDEA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.184"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IV136_002824"
     CDS             2..400
                     /locus_tag="IV136_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8466726.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IV136_002825"
     CDS             384..1079
                     /locus_tag="IV136_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8466727.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          609 a          232 c          321 g          451 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata aga
//
LOCUS       DACDEA010000179         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDEA010000179 DACDEA010000000
VERSION     DACDEA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.185"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..453
                     /locus_tag="IV136_002826"
     CDS             <1..453
                     /locus_tag="IV136_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP8466728.1"
                     /translation="KNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYS
                     GLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNV
                     FKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            698..>1564
                     /locus_tag="IV136_002827"
     CDS             698..>1564
                     /locus_tag="IV136_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8466729.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          617 a          184 c          317 g          446 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
      361 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
      421 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
      481 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
      541 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
      601 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgaccaa
      661 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
      721 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
      781 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
      841 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
      901 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
      961 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     1021 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     1081 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     1141 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     1201 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     1261 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     1321 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     1381 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     1441 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     1501 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     1561 tgat
//
LOCUS       DACDEA010000180         1462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDEA010000180 DACDEA010000000
VERSION     DACDEA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.186"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            221..655
                     /locus_tag="IV136_002828"
     CDS             221..655
                     /locus_tag="IV136_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8466730.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IV136_002829"
     CDS             678..998
                     /locus_tag="IV136_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466731.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IV136_002830"
     CDS             1139..1330
                     /locus_tag="IV136_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466732.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          571 a          215 c          308 g          368 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt ca
//
LOCUS       DACDEA010000181         1440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDEA010000181 DACDEA010000000
VERSION     DACDEA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.187"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IV136_002831"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IV136_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IV136_002832"
     CDS             complement(546..740)
                     /locus_tag="IV136_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466733.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          508 a          229 c          191 g          512 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
//
LOCUS       DACDEA010000182         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDEA010000182 DACDEA010000000
VERSION     DACDEA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.188"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV136_002833"
     CDS             complement(150..1337)
                     /locus_tag="IV136_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8466734.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDEA010000183         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDEA010000183 DACDEA010000000
VERSION     DACDEA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.189"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..923)
                     /locus_tag="IV136_002834"
     CDS             complement(<1..923)
                     /locus_tag="IV136_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8466735.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
     gene            complement(934..1140)
                     /locus_tag="IV136_002835"
     CDS             complement(934..1140)
                     /locus_tag="IV136_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8466736.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1273..>1371)
                     /locus_tag="IV136_002836"
     CDS             complement(1273..>1371)
                     /locus_tag="IV136_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466737.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          389 a          286 c          222 g          474 t
ORIGIN      
        1 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
       61 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      121 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      181 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      241 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      301 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      361 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      421 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      481 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      541 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      601 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      661 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      721 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      781 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      841 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      901 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      961 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
     1021 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
     1081 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
     1141 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
     1201 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
     1261 ctttcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac tcaaaaatac
     1321 ttccaaaggt gttcgataat ccagtgattt tctaggaata ttatttcgtt t
//
LOCUS       DACDEA010000184         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDEA010000184 DACDEA010000000
VERSION     DACDEA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.190"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(950..>1366)
                     /locus_tag="IV136_002837"
     CDS             complement(950..>1366)
                     /locus_tag="IV136_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8466738.1"
                     /translation="KRVEEVDRTPAENLAYQVGWTNLILKWENDERNGLSVKTPSDQF
                     KWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDLFTAHKRKWADE
                     ATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          431 a          265 c          202 g          468 t
ORIGIN      
        1 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
       61 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
      121 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
      181 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
      241 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
      301 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
      361 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
      421 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
      481 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
      541 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
      601 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
      661 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
      721 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
      781 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
      841 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
      901 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
      961 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
     1021 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
     1081 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
     1141 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
     1201 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
     1261 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
     1321 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tctttt
//
LOCUS       DACDEA010000185         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDEA010000185 DACDEA010000000
VERSION     DACDEA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.191"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1362
                     /locus_tag="IV136_002838"
     CDS             82..>1362
                     /locus_tag="IV136_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8466739.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DACDEA010000186         1361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDEA010000186 DACDEA010000000
VERSION     DACDEA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.192"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1330
                     /locus_tag="IV136_002839"
     CDS             29..1330
                     /locus_tag="IV136_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8466740.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          442 a          274 c          252 g          393 t
ORIGIN      
        1 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca aactactact
       61 tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca ttaagaatat
      121 caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc ctttgtgtaa
      181 acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc tcattcaact
      241 taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct accactgtcg
      301 tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact gctttatcgc
      361 taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa tgacgacaat
      421 tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt ctctgatccc
      481 catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg aatttcgttc
      541 actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata ctggaaaatt
      601 actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta tgcaatttga
      661 acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt cttatgcctc
      721 tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc acattgtcaa
      781 acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct ttgatcgaaa
      841 cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc ttctgatcaa
      901 acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg gttgggcgta
      961 tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat tacgaatcgc
     1021 ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg acactttctt
     1081 taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta taaaaaaagc
     1141 ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt caaatgccaa
     1201 aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg gctttcgaag
     1261 ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct atgaatcaaa
     1321 aaacatctaa agtagaaaag gaattctacc ttagatgttc t
//
LOCUS       DACDEA010000187         1315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDEA010000187 DACDEA010000000
VERSION     DACDEA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.194"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..563
                     /locus_tag="IV136_002840"
     CDS             90..563
                     /locus_tag="IV136_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8466741.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            572..799
                     /locus_tag="IV136_002841"
     CDS             572..799
                     /locus_tag="IV136_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466742.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          416 a          202 c          215 g          482 t
ORIGIN      
        1 atatttttat tgtttttcga taaaaaatag ttgttttaca ataattattc tgctatattt
       61 tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg
      121 tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg
      181 ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt
      241 actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa
      301 tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa
      361 ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg
      421 atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg
      481 cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga
      541 cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt
      601 attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc
      661 aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa
      721 aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa
      781 tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg
      841 atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat
      901 tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg ggccttttct
      961 tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc
     1021 tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa
     1081 taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct
     1141 aatggcccat tgtgttaact cttactccat tcttttttag taagctccca tatatactgt
     1201 aaagccttct atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa
     1261 atgatttgaa taacagtgat ggacaattct tttttatttt taacattatt ttgat
//
LOCUS       DACDEA010000188         1274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDEA010000188 DACDEA010000000
VERSION     DACDEA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.195"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1274
                     /locus_tag="IV136_002842"
     CDS             <1..>1274
                     /locus_tag="IV136_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8466743.1"
                     /translation="SIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTCRN
                     CGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYF
                     VQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKF
                     LVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAE
                     KLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYF
                     HELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIE
                     AKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          425 a          244 c          245 g          360 t
ORIGIN      
        1 attctatcaa aaaaatgctg cgattaatag agaaagattt gatgattaca gaggtctctt
       61 acgagacctt tcagaagaaa aagacgttga tcgtcgatgc tgttttctca cctgctcccc
      121 atacctgtag aaattgtggt tctactgtgg tagatggaaa cgggaaagta attgtagtga
      181 aaaatgggaa gaaggaaacg attgtccgct ttgaacaata caatcacatg cctttggtta
      241 tgcgcttaaa aaagcagcgc tatacctgta aaaactgtcg aacccattgg acggctcaaa
      301 gttattttgt tcaacccaga cattcaatcg cgaatcatgt cagatataaa attgcttctt
      361 tactgactga aaaagtatct ttatctttta ttgcgaaaaa ttgtcaggta tctttgacca
      421 ccgttattcg tacgttgaaa gagtttaaaa gctacttacc aaagcaatct aagaggattc
      481 tccccagagt attgatggtt gatgaatttc gttcgcacgc ttccatagaa gataaaatga
      541 gctttatttg cgcagatggc gaaacaggac aattaattga tgtcttgcct acgcgtaaat
      601 tgcctcgatt aacaagctat ttcttaggct gcgctaatcc tgaagaagta aaatttttag
      661 tcacagacat gaacgccgcc tacttccagc ttaccaaacg tattctgcca aatgcgaaag
      721 tagtgattga tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta
      781 tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa
      841 agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat
      901 ggaaaagttt tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat
      961 tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag
     1021 cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt
     1081 tagatgacgg ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca
     1141 acgcgatgat ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga
     1201 caatgaaacg agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt
     1261 tgatcaatca atta
//
LOCUS       DACDEA010000189         1247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDEA010000189 DACDEA010000000
VERSION     DACDEA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.196"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..419)
                     /locus_tag="IV136_002843"
     CDS             complement(<1..419)
                     /locus_tag="IV136_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8466744.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLE"
     gene            complement(578..844)
                     /locus_tag="IV136_002844"
     CDS             complement(578..844)
                     /locus_tag="IV136_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466745.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(834..1190)
                     /locus_tag="IV136_002845"
     CDS             complement(834..1190)
                     /locus_tag="IV136_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8466746.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          341 a          249 c          155 g          502 t
ORIGIN      
        1 tcttctaaag agtccctgta cttcttaact tctatatact cttcagccaa aacttgtgga
       61 actggttcat ctaagaaaaa aacccctacc tcgcctttgc tatttttata aaagtaatca
      121 atttctgcta tagttcccga tctataatta tcaactggag aacccaactt atggccaaaa
      181 atcgctaata atatatcact attttctaca atttgtttat taataacact ttgcccagag
      241 ctattattta tataagtagg aatcgcattc gtttcccatc gaattggcat caaaattact
      301 tttgtattat ctgtattttc tatattccat ctatatatgg tttcttctac ttcattcctc
      361 tttgctgcta tatcacctgg tgatgctata aatactttta ttagatttat ggttttcatt
      421 tattgtacct ttctctttat gtctctttaa agtaattttt gtataaaatt tattgacttt
      481 tacactatag gacaactttt atctatttag tacaaaaagg cccactaaac taattgatta
      541 gttaggagcc tattacaggt atattttcaa ctcgctttta agcaaattct actaccagct
      601 ttttgcctaa tgccctagcg atatcatcaa gtttatcgaa agatacgtta tcccctctct
      661 caattcgagc aatagtagat tgagttgtat gtgctttttg tgcaagctcc gcttgagtta
      721 ggcctgcttc ctcccgtgca tgataaagag caaccgcagt ttcgagtttt cttccttcac
      781 ggtcatatgc ttctgcaaaa tctttgtctt tttgcttttc ttcaatcaat tttttaatca
      841 tcatttgtga accactcctc tctaagttct tttgcatgtt taagttcact tattggtgtt
      901 ttctccgttt ttttagtaaa tccatgggta atgacatacc ttccattgac cacatggaaa
      961 tagatagctc tttggatgtt tgatcctacc ttacttctta attcgtacaa attagtgtct
     1021 aattttttca cccatttcaa ccgttgagct actagcatcc cattcttttc tgtttcttct
     1081 attgtacgta aaagcttcgc actatctttt tttggtaatg tttccaacca ttctaaaaat
     1141 tcatcatgac cattaggtct tttaaatgtt tcaaaagata ttttttccat acccaattat
     1201 atcatatatt gattttatat tatagtatat aacataaaaa ttcgaat
//
LOCUS       DACDEA010000190         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDEA010000190 DACDEA010000000
VERSION     DACDEA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.197"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV136_002846"
     CDS             200..742
                     /locus_tag="IV136_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466747.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV136_002847"
     CDS             832..>1246
                     /locus_tag="IV136_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8466748.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDEA010000191         1235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDEA010000191 DACDEA010000000
VERSION     DACDEA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.198"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..338
                     /locus_tag="IV136_002848"
     CDS             78..338
                     /locus_tag="IV136_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466749.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(700..939)
                     /locus_tag="IV136_002849"
     CDS             complement(700..939)
                     /locus_tag="IV136_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466750.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          434 a          170 c          228 g          403 t
ORIGIN      
        1 tgttattgat tttcataatc atattttaat ggtattatca ttttaaataa tggaaaaaca
       61 ttaaggatgg atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa tatatttgcc
      121 aacctattgg cttgaaacaa aaagtggttg gagaagtaat cagtaaatta gaaaattgtg
      181 ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta gaaaaatgcg
      241 gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa tatctatata
      301 actatagcaa caaatatgaa catactttag tttcataaag atttaaataa agtgccttca
      361 aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat tgttcatgat
      421 ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg cctggtggag
      481 aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac aaactagtag
      541 ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata tcaggatcat
      601 ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta ggagtaaacg
      661 cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag tttgtttgaa
      721 ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag aaacttttat
      781 atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat cgatactatt
      841 cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag cagtaagtaa
      901 caataaattt gataaactga taaacagaat tcttttcatt atgtacacac tccttatagt
      961 atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac tatcaagtct
     1021 tttatagtat ctattagtct tagtaataca tatatggaga gggtgatatc ccagttcaaa
     1081 ccagatacat tggttaccat ctgtattaac gattatgcct ctaaacgctt tttttatgaa
     1141 aaaatttata agtgaaaacg taggtatgat attatctaga ggtaattttt ttgggtattt
     1201 aaatagttgc taggaggata gctgggagcg tcaat
//
LOCUS       DACDEA010000192         1221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDEA010000192 DACDEA010000000
VERSION     DACDEA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.199"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..346
                     /locus_tag="IV136_002850"
     CDS             134..346
                     /locus_tag="IV136_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466751.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..1026
                     /locus_tag="IV136_002851"
     CDS             508..1026
                     /locus_tag="IV136_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP8466752.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
BASE COUNT          475 a          149 c          191 g          406 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ctagacatat taaatatgta tagagatatt
      721 acttttagta aaaataaatt aaaagatata aattcaattg ataattatta tatacaattt
      781 aaaggttttg actttaatag ctctaccgaa tttaagttag cactttatgc acaatttttt
      841 attgaaaaat taaataggtt tcaagaaata gttgaagata gtgattttaa cacttttaat
      901 agccataaac cgatgagagg tacttatata aaatttctcg aaaattataa tgatttaaaa
      961 tctaccaata attatcaatt tggaaacttg tcttatgaga tgataagtaa agttctaagt
     1021 ttgtgatgat aaatgaaatt aaatagtata aatgatttat ttgctattat aggtgttatt
     1081 gctaacatac ttttttcgtg tttgaatttt agatttactc aaaagaacaa aaagaacaaa
     1141 ctcagtatag aacaaatgaa aaaagacatt gaaactgatt taaaaaatga ccataaagaa
     1201 agaagaaaaa ttgaagaata t
//
LOCUS       DACDEA010000193         1202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDEA010000193 DACDEA010000000
VERSION     DACDEA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.200"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1061..>1202
                     /locus_tag="IV136_002852"
     CDS             1061..>1202
                     /locus_tag="IV136_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8466753.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
BASE COUNT          430 a          198 c          195 g          379 t
ORIGIN      
        1 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
       61 tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga
      121 gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt
      181 cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta
      241 agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac
      301 ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc
      361 gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg
      421 gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta
      481 aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct
      541 gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact
      601 acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg
      661 caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa
      721 tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa
      781 ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg
      841 tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta
      901 tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt
      961 tttagtgaac tacccccact tagcttggat aggctacaat taactagaca gaaaaattaa
     1021 ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga gaacacgaag
     1081 aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg gaaagccacg
     1141 tgtagaaatc attcgagaat atgaactaac ggcttcagca tttgacaaat gggtaaagca
     1201 at
//
LOCUS       DACDEA010000194         1182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDEA010000194 DACDEA010000000
VERSION     DACDEA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.201"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..>1182)
                     /locus_tag="IV136_002853"
     CDS             complement(23..>1182)
                     /locus_tag="IV136_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8466754.1"
                     /translation="VPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTL
                     EGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLT
                     LFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYF
                     FYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYF
                     LVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQIS
                     QYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHAD
                     LIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          377 a          168 c          216 g          421 t
ORIGIN      
        1 agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa ccttttttca
       61 tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg tgcgttgtat
      121 ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta atgatcaaat
      181 cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac tgctgcaaat
      241 ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg atgagtaact
      301 gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat tgactgattt
      361 ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat ttcttttcct
      421 gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac
      481 tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa
      541 ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa gtcatcaatt
      601 gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca atctcctgtt
      661 tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta aagtaggctc
      721 tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag tattctgaat
      781 aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa gtttttggaa
      841 aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag atattttttt
      901 cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt gcatttaatt
      961 caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa aagtaaagat
     1021 agagacggat agataactca tttccttcaa gagtacagca attttttaca gataggatcc
     1081 gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg agataggagg
     1141 gcgatatata aagttgttca agtaaatatt tttttggtac ag
//
LOCUS       DACDEA010000195         1178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDEA010000195 DACDEA010000000
VERSION     DACDEA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.202"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            76..>1178
                     /locus_tag="IV136_002854"
     rRNA            76..>1178
                     /locus_tag="IV136_002854"
                     /product="23S ribosomal RNA"
BASE COUNT          363 a          229 c          334 g          252 t
ORIGIN      
        1 ttttaataag ttcaattgct tattttcttg atctaacttc tatcgctaga agaaggatca
       61 aaacccaacc gtaaggttga taaggttaag tgaataaggg cgcacggtgg atgccttggc
      121 actaggagcc gatgaaggac gggactaaca ccgatatgct ttggggagct gtacgtaagc
      181 tatgatccag agatttccga atgggggaac ccaacatctt taataggatg ttacgattgt
      241 gtgaatacat agcacattcg aggtagacgc agagaactga aacatctaag tacctgcagg
      301 aagagaaaga aaattcgatt ccctgagtag cggcgagcga aacgggaaaa gcccaaacca
      361 gcaagcttgc ttgttggggt tgtaggactc caatatggta gttctttcag atagtcgaat
      421 gacttggaaa agtcagtcaa agagggtaaa aaccccgtag acgaaatgtg gaagacacct
      481 aggaggatcc tgagtacggc ggaacacgag aaattccgtc ggaatccggg aggaccatct
      541 cccaaggcta aatactccct agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa
      601 aagcaccccg gaaggggagt gaaatagaac ctgaaaccgt gtgcctacaa caagtcaaag
      661 cccgttaatg ggtgatggcg tgccttttgt agaatgaacc ggcgagttac gattgcatgc
      721 gaggttaagt tgaagagacg gagccgcagc gaaagcgagt ctgaataggg cgtttgagta
      781 tgtagtcgta gacccgaaac catgtgatct acccatgtcc aggttgaagg tgcggtaaaa
      841 cgcactggag gaccgaaccc acgtacgttg aaaagtgcgg ggatgaggtg tgggtagcgg
      901 agaaattcca aacgaacttg gagatagctg gttctctccg aaatagcttt agggctagcc
      961 tcggaattga gaatgatgga ggtagagcac tgtttggact aggggcccat ctcgggttac
     1021 cgaattcaga taaactccga atgccattca ttcatatccg ggagtcagac tgtgagtgat
     1081 aagatccata gtcgaaaggg aaacagccca gaccaccagc taaggtccca aaatatatgt
     1141 taagtggaaa aggatgtggg gttgcacaga caactagg
//
LOCUS       DACDEA010000196         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDEA010000196 DACDEA010000000
VERSION     DACDEA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.203"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1129)
                     /locus_tag="IV136_002855"
     CDS             complement(<1..1129)
                     /locus_tag="IV136_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8466755.1"
                     /translation="MSWIEETIDSMTLEEKIGQLFVNMGSSRDKNYLSNMVNQYHIGA
                     VRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDA
                     KYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYM
                     KGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSI
                     MAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMT
                     GAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLK
                     AKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAIT"
BASE COUNT          361 a          235 c          150 g          388 t
ORIGIN      
        1 atgtaattgc ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt
       61 tttccagagc ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca
      121 atttagcttt taaccctaaa attcttttta aggcatctaa taaccgatca tcagtgataa
      181 ttcctttacg atagccttcc atcatataat taaagtcttc atctggatca ttaaaaaata
      241 gaaatagatc acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc
      301 cagtcattcc taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc
      361 ctctaagtaa gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt
      421 tcagatcagg attaaaatgt tttgaataag ctggcatatg gatatgacct gccataattg
      481 aaggtaatcc agcttctatt aagcctgagt atacttttcc aaaagaatta tcccattctt
      541 ccgtacctaa actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac
      601 cagggaaatg tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta
      661 aactaagttc taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat
      721 ttctccaatt tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg
      781 atgcttcaat cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac
      841 caatttttac ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt
      901 ccgtattagc agcaattaat agaggaattt tactttctgt ttgtaagata tagttttgat
      961 catatacctc atcagcagtt cctggattat atcgtacagc tccaatatgg tactgattga
     1021 ccatattact caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta
     1081 ttttttcttc caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcat
//
LOCUS       DACDEA010000197         1081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDEA010000197 DACDEA010000000
VERSION     DACDEA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.204"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..447
                     /locus_tag="IV136_002856"
     CDS             <1..447
                     /locus_tag="IV136_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466756.1"
                     /translation="IREVRKNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLM
                     NIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEF
                     GKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          386 a          129 c          231 g          335 t
ORIGIN      
        1 atacgagaag tccgaaagaa ccgggaagag acgcttgaaa aatttggtaa aaactttacc
       61 atctatacag gcaaaaatgt tataagtaga tgggaaaaag gtattaatat tccagatatt
      121 gaaaaactaa tgaatattgc ctatcttggt aaggtcactg ttccttacct tatgtacgga
      181 gaagattata agaatatact tccaaaagat gaaagagtta gcgattttaa aaagataaat
      241 tcttttagta tgggattaag gatgaggaaa attaggaaag attattatct agaaagggag
      301 gagtttggaa agcttttttc tccttcaatt tcaaaatggt ccatagatag gtatgagaat
      361 ggtagagata tacccaatac taatcgaatt atccaatatg cttatatagg aaatctttct
      421 ttagaatttt tgatttacgg gatataaaaa tagtttacgt cgatacttat taagttatcg
      481 acgtttttta ttatagaaaa aatttataaa aggaggtttc ttgatatgat aagaaacaaa
      541 aagggtttgt tgaaagttgc tttactggca gcttttttct aagtaacttt ttgacaagct
      601 caatggcaac acaagcggaa gaagtaatac aagaactaac tgttatgaat aacagctaga
      661 taaaatgggc tatgtatgtt atccaacaat ttagtaatca ctagaagcct agcgaaagtt
      721 cgcttgataa gttatagatg agtattgatt tgtagtgttt ccaaatcaaa agggtacaga
      781 gccacatgaa atatgttgtc ctctgcagcg tcaggatggg aatccatacc atggagattc
      841 ggagatctgt gagggaatac ttacgtggtt aatatgtcag ttgtgttgga cgtagtaatg
      901 gtcatagtgg gtagattgac tgaattctta tatgagaaaa tgaaaccaac aagaagatag
      961 ggttatttat acagaattgg ggatcttttc tggaaaagta atcggataat gcgagattcg
     1021 tacggtcttt tttaaatttc tctaaaaaaa acggcatcga atgggttggg tgattgataa
     1081 g
//
LOCUS       DACDEA010000198         1080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDEA010000198 DACDEA010000000
VERSION     DACDEA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.205"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            239..322
                     /locus_tag="IV136_002857"
     tRNA            239..322
                     /locus_tag="IV136_002857"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Leu,seq:cag)
     gene            complement(646..>1080)
                     /locus_tag="IV136_002858"
     CDS             complement(646..>1080)
                     /locus_tag="IV136_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8466757.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          350 a          177 c          238 g          315 t
ORIGIN      
        1 agaggaaaaa agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt
       61 aacattgaaa tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg
      121 gagttgctcg gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa
      181 agaacaaaaa tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc
      241 ggctatggcg gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga
      301 agttcgagtc ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc
      361 gatgagaaag aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa
      421 ccagattcaa ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag
      481 attgattcaa atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta
      541 aggaataaag actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac
      601 ctcttactat attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt
      661 tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc
      721 tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg
      781 gtaacggccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc
      841 gatccattct tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg
      901 agcaggttgt gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac
      961 aacaggttgt tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg
     1021 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACDEA010000199         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDEA010000199 DACDEA010000000
VERSION     DACDEA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.206"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..274
                     /locus_tag="IV136_002859"
     CDS             <1..274
                     /locus_tag="IV136_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466758.1"
                     /translation="RKSDEIITRENIDLENEISKLESIFSDINSLKYLIVEENSDSFL
                     EFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLRY"
     gene            545..>1070
                     /locus_tag="IV136_002860"
     CDS             545..>1070
                     /locus_tag="IV136_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466759.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          401 a          143 c          186 g          340 t
ORIGIN      
        1 aagaaaaagt gatgagataa tcacacgtga aaatatagat ttagaaaatg agattagtaa
       61 acttgaatct atcttttccg atataaattc tcttaagtat ttaatcgtcg aagaaaattc
      121 ggatagcttt ttagaatttg aaaaatatct aacagatctt atagataata gaaataatga
      181 attaatgaga caaaacattg caagattcct tactaaatta gtttctgaat ggaaaagtat
      241 tatttctcaa aatatggata aattaagata ttaaggatgt tagttttaaa ataaactaat
      301 gtccttattt ttgtgttcac atttctgtta cctaagaaat acttaaagga ggtgatgtag
      361 ccagcaacga ctacaacttg aacccgctat ctacagttta tttgttgttt cttttgggta
      421 tactctaatg gtacaaatcc atatttgctt aaagcgttga tttttcagcg gtttttcttt
      481 attgcaaagt gtaccctaag acctgtatac taatagtaaa tagattggat aggaatgatg
      541 gcaaatgtat ttagcttatt taagagtttc ttccgacgat caaagtttag ctcggcaaca
      601 taagctaatt gaaaattggt cagtcaaaaa taatattgct gaagacgatt taaaaatttt
      661 cgaggaaaaa gtctctggaa aaaatattga aaacagacca gagctgcaag agcttatttc
      721 tttcatacgt gaacgagata cgattattat tcctagtctt gatcgattag caagaaatag
      781 taaagataca aaagacttac ttcaattgtt tcgtagtaaa ggtgcttcta ttgaaatatt
      841 agacctaccg tcgtttaatg gggtaactga ccctgcatta agagacttgc taacaaattt
      901 agtcatagaa gtgtttagct atgttgctga aaacgaacgg aaaaaaatca aagagcgtca
      961 aaaacaaggc attgaaattg caaagaaaaa tggtcgttat actggtaaaa agattcaata
     1021 ccatgccaat gcaacaggaa aaaacaaatt gatttacgat gaaattgttg
//
LOCUS       DACDEA010000200         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDEA010000200 DACDEA010000000
VERSION     DACDEA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.207"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(39..995)
                     /locus_tag="IV136_002861"
     CDS             complement(39..995)
                     /locus_tag="IV136_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466760.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DACDEA010000201         1033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDEA010000201 DACDEA010000000
VERSION     DACDEA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.208"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..489
                     /locus_tag="IV136_002862"
     CDS             196..489
                     /locus_tag="IV136_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8466761.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          389 a          149 c          182 g          313 t
ORIGIN      
        1 atttgaacac cctgatccag ttttttcttt aattattggt aggggagaat aatggattag
       61 aataaatcta cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag
      121 agtaaccgct ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc
      181 aaaaggagaa agaagatggt ttatacttat acggagcaag aaaagatgaa agttttagac
      241 acattcatga atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaatacatc
      301 ctattgaaag aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt
      361 aattcgatac tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta
      421 atcacatttg gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt
      481 gaaaattaga agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc
      541 tacaccgcat acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt
      601 attcaaaata tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat
      661 cgtcagtaaa atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat
      721 aaagataatt ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat
      781 tttggacaga tgccctttaa aaagataaca agatttcgtt cttgaaacaa tatatatgct
      841 tttattccaa tgaggttgtg aaaacagtat tttgtcctga gcgttagaaa attgaaaatg
      901 gggaatagca actcgtactt taccattttt taagtgaata caagatcagc tagttgaaca
      961 ccgtttattc gtgatgaggg acacaacact gcagtcgaca catttctctt ttatgttcaa
     1021 agataacaaa tat
//
LOCUS       DACDEA010000202         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDEA010000202 DACDEA010000000
VERSION     DACDEA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.209"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            369..1002
                     /locus_tag="IV136_002863"
                     /pseudo
     CDS             369..1002
                     /locus_tag="IV136_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACDEA010000203          999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDEA010000203 DACDEA010000000
VERSION     DACDEA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.210"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..421
                     /locus_tag="IV136_002864"
     CDS             11..421
                     /locus_tag="IV136_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8466762.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            435..791
                     /locus_tag="IV136_002865"
     CDS             435..791
                     /locus_tag="IV136_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8466763.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          340 a          156 c          195 g          308 t
ORIGIN      
        1 gagtgagaaa atgataaaag tatatacttc gcctagctgt acttcctgta gaaaagcaag
       61 agcatggttg caagcgaatc aacttgaatt tgaagagaag aatatttttg ccgaaccgtt
      121 aacagaaagt gagatcaaaa agatcttggc agcgactgaa ggaggagttc aagatattat
      181 ttccactcgg tcgaaaattt acgaaaagct gggcatagat tttaacgaat taactttaaa
      241 gcaaatgatc gtcttattca aagaatatcc ttcattattg agacgaccta ttttgattga
      301 tgaaaaaaga atactaatcg gatttaatga agatgagatc cggtcatttg tccctcgtga
      361 aattcgatgt gttgccagaa atctgatcta tttacaaaac aataacatct taaattcata
      421 gggagggagc cttcgtggaa gtgaaagtct atggatcagc gcactgtgta acaagtctga
      481 aggcgaaaca atggttagaa caccatcaat tgccttatca atttgttgac ttagaagaac
      541 gcgatctaag tacagaagaa gcagaagaat tatgcagttt tgaagaagtg caagcagaac
      601 agctatttgc gacttggtct gaggcttttc acaacattgc gattgacctt tcctgtgtag
      661 gaaaaaaaca actgattttt ttatgcacac aacataccaa tctcctcaga cgtcctttga
      721 ttattatcga tgaagttcta tttattggat acaatgagca gttgatggaa cagcttatct
      781 taaataaata gactttaact gttttaatca agtcactgag catttcatac agttgtaaca
      841 gtcgaacact tagcatcaag cgtgttatgt gttctttttt tgactttatt tttctactat
      901 agagacatgc tcaatttatg tggtcaaaat tcaaatctag gtggggatat agagttaaaa
      961 atatattgac atgtgtaaat ttacatgata tattcattt
//
LOCUS       DACDEA010000204          971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDEA010000204 DACDEA010000000
VERSION     DACDEA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.212"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..971)
                     /locus_tag="IV136_002866"
                     /pseudo
     CDS             complement(1..971)
                     /locus_tag="IV136_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          247 a          200 c          179 g          345 t
ORIGIN      
        1 ttggacaaga ctttgccagt ttaatttctg ttttatccgt tcattattgt agtaatagat
       61 atagctatca atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat
      121 ctcttgtttt aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt
      181 ccgagacatg ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg
      241 atacgcccaa ccttgatcta agtgaaacat acgacgatag ggacaatcgt tagtggtctg
      301 gacagcttcc tccaaacctt ccattaccgc taatgcattg ggcttttcag agatacgata
      361 ggataaaatc tcactattga acatatccat aaaaggatct agatacaatt tcttttgacg
      421 gataatgcct ttggcatccg cttcatggta tttaaattca gttgtatccg tcgtgatttt
      481 ctgatggcag atactggtgt agaaacgacg atggatccga tttttggcta ctgctccaac
      541 ttgtcctcga taagaattgt agcgacgtga tttacgtgtg tatgccgtta cttttattcc
      601 tagcttcttg atcaggcgct gaactttctt tttattgata gagaaaccgc ggtttcttag
      661 ttcattggtc atccgtaaac agccataatc cttgtgcttc tcccgaattt taagcatttc
      721 cgcttcaatc tcttgatctg gatttttacg gtccaatcgt ttttgccagt acataaaggt
      781 tgccttaggg aactttaaag tttctagaag ctcaactaat ttaaacggtc ctcggaggct
      841 tgcgatgatt cgtgcgattg cttcattcgt cgttgttggg cttcctgttt tcgcagcttc
      901 ctcaattctt ttaaaaaggc attttcaatt tgaagggaac ggactcgttt ctccaactcc
      961 tcgatgcgtt c
//
LOCUS       DACDEA010000205          968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDEA010000205 DACDEA010000000
VERSION     DACDEA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.213"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..>968
                     /locus_tag="IV136_002867"
     CDS             4..>968
                     /locus_tag="IV136_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8466764.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIK"
BASE COUNT          342 a          129 c          170 g          327 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaata
//
LOCUS       DACDEA010000206          961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDEA010000206 DACDEA010000000
VERSION     DACDEA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.214"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..286
                     /locus_tag="IV136_002868"
                     /pseudo
     CDS             1..286
                     /locus_tag="IV136_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            320..>961
                     /locus_tag="IV136_002869"
     CDS             320..>961
                     /locus_tag="IV136_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8466765.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKL"
BASE COUNT          352 a          110 c          180 g          319 t
ORIGIN      
        1 ggcggatatc agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt
       61 tttgctgtct aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa
      121 tgagatttgg agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat
      181 actttttctt gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat
      241 gcacggtaaa ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata
      301 tgtttatgag gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat
      361 caacaatttt tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg
      421 atcattggaa caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt
      481 aaacaatgtt tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc
      541 aattagtcat ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa
      601 aggattatca caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga
      661 aaaagttaag gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga
      721 ttttaacgca ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat
      781 ttgtgttctt tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa
      841 tctaattaat gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg
      901 tgagttctgc ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct
      961 t
//
LOCUS       DACDEA010000207          952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDEA010000207 DACDEA010000000
VERSION     DACDEA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.215"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>952
                     /locus_tag="IV136_002870"
     CDS             <1..>952
                     /locus_tag="IV136_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8466766.1"
                     /translation="DSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPP
                     NGLPLIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLF
                     GYKKGAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESV
                     QWEYANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEA
                     ITIDSKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDS
                     GFWIDPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQ"
BASE COUNT          339 a          134 c          173 g          306 t
ORIGIN      
        1 gattctgtta gaccagtaat gttcaagtat aatcaacaaa agccaaaaga ctgcttttct
       61 aagtttattg gagcagacat atctttgaaa tccactatag ataaatgcaa agctactgta
      121 atgtatcccc ctaatggcct cccattaata ataaaaggaa acagtggcgt tggaaaaagt
      181 tttttagcaa gtttgatcta tcagtatgct cttgatagaa aagtaattca taatgacgca
      241 aaatttgttg ttgtgaattg tgcagattat gcaaataatc cagagctatt gtcagcagtt
      301 ttatttggat ataaaaaagg agcctttact ggggctgaaa aagatactgc agggatgcta
      361 tcttctgcta atggaggata ccttttttta gatgaggttc acaatctaag tgcagaaaac
      421 caagagaaat tgtttcttct tatggattct caaaagtatc gtaaacttgg tgaaagtgtg
      481 caatgggagt atgcaaatgt tcgccttatc ttagccacaa cagaagataa aaatacctct
      541 ttgttagcaa cttttagaag acgcatccca gctgaaatca ctctgccaga ttacaataat
      601 agaagtacat ctgaaaaaat tcaattatta tttgaatttc ttaaagatga agcaattaca
      661 attgattcaa aaatattttg ttcgattgaa ctttttactg atcttttatc gaaaacattt
      721 gaaggaaatg ttggagaatt gaggaatgaa atcaagcttc tttgtgcaga aggttattta
      781 ggaaataaga gaaagtcttc aatttattta ggggacaaac tagacagcgg tttttggata
      841 gatccagaaa ttagtataga tttaaaaaaa atgtttctta aatcactttc tgaaattgat
      901 ttgattgaac tttttcaaaa tcacatttca attgaagaaa gcatgagtca at
//
LOCUS       DACDEA010000208          933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDEA010000208 DACDEA010000000
VERSION     DACDEA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.216"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..768)
                     /locus_tag="IV136_002871"
     CDS             complement(<1..768)
                     /locus_tag="IV136_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8466767.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL"
BASE COUNT          390 a          140 c          147 g          256 t
ORIGIN      
        1 taaagaagaa aaaccattta accgccagac aaattgtaga ctcttaactg gtgtatggtt
       61 aaaaagttgc caaggaaata aattggttga acaaaaaaga acaaatacac caagaaagaa
      121 aataaataaa tcatcagcag ttaatttttt tataaaaata aaagcaccaa tcaatccgag
      181 aaataagaat aatccaatag tatagttgtt atagttatta tttaaaatat gtgtcagcat
      241 atcactagga gccataccac ttaattcttt tcccagtggg actttcaatt cttgtgctct
      301 agattgttga ataaaaggaa ttaagaatcc cgcacttaaa aaaaaggtga ggacggtagc
      361 ttttaaaaga cttaagagtc tagcgatttt ttggtcccaa aaataaaaag aaagaaacaa
      421 cgttcctcca ataaatacag aaaccatagc gacagaaagt aaatgagtat acactactag
      481 cgtcatgcca atagacaacc agtaccattt ttggtaatct ctaatataaa tttcgtaaca
      541 ccccattagt atcaaaggca aaaatgttaa agcgacagct tctcccaatg aagcacgatg
      601 gaatatgtca atggcacgat aggcggaaaa tgtataaata atcgaaaata acagcgaaat
      661 ataacgattg ttttttattt gtttcataga aaaataagaa acgaccattg ttaaaaaagt
      721 gataaaaaaa tagtagatat tataacttaa aacaagattt cccatcattt tataaaataa
      781 aaaagctgga taaagagtaa gccatggata aaaaatattc atcattgttc catgatgatc
      841 gaagttattg aagtttactg gagaactcca tacattatct agtcctatga ttcgagataa
      901 atgaaagtaa gtatcctgat tgacactatg ata
//
LOCUS       DACDEA010000209          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDEA010000209 DACDEA010000000
VERSION     DACDEA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.218"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..254)
                     /locus_tag="IV136_002872"
     CDS             complement(<1..254)
                     /locus_tag="IV136_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8466768.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="IV136_002873"
     CDS             complement(254..517)
                     /locus_tag="IV136_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8466769.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..888
                     /locus_tag="IV136_002874"
                     /pseudo
     CDS             751..888
                     /locus_tag="IV136_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          294 a          180 c          130 g          322 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaagta acgcgataca
      901 gtgattattg cttgtgttat atgggg
//
LOCUS       DACDEA010000210          912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDEA010000210 DACDEA010000000
VERSION     DACDEA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.219"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(72..302)
                     /locus_tag="IV136_002875"
     CDS             complement(72..302)
                     /locus_tag="IV136_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466770.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(396..734)
                     /locus_tag="IV136_002876"
     CDS             complement(396..734)
                     /locus_tag="IV136_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466771.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          290 a          187 c          102 g          333 t
ORIGIN      
        1 atattaacag caaagatatc gaaaaaacaa taacataata tgtgtcaaaa tttttcataa
       61 taagaactcc cttactgttg catactaaca taatccaaaa actcttctac ttcagctaaa
      121 gacattccac tttctttagt ccaaaaaaca gttttttcta tcgatccttc tctgtaaact
      181 gtaaatgtcg gagtcgagtt aattgagtag cgactcttga attctttcca ctcattttca
      241 tcttctctaa cttttgctac attcacgtaa taaatatttt cttttccctt ttctaccagc
      301 atatcgaata gcttcctgct tttgatgatc gatttcttgt gctacatcta gtaaattttc
      361 atcaatttct atatcataaa gtatttcact agtgtctaca tccacaatat atacttttta
      421 tcagtagcaa tttttgagta atccgttaaa atgatctcag tctcgtcact ttcatcagta
      481 taaatatacg ttaacaatga actcaccttt tcaatcggtg ttagctctaa gcctactgta
      541 ttttttacta ttgagtcatt atttaccacc ataacttcgt actgtatata ccatcctatt
      601 tggtttccat caatatcagt aattggctca ctagctaatg gagtctccgg aacaatattt
      661 tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt tatcatgttc ctcacttgaa
      721 actggttgag tcatagaaaa tgttacttgg ctaaacagag ggaaaataaa cactacaatc
      781 aacaaagtat aaaaaaaagc ccttcttttt cattttttat cttccaatca caaattgaaa
      841 aacttattcc tgcaactttg agtctagtat cacaatcgac tgactacaac tattcattta
      901 cattctactt ta
//
LOCUS       DACDEA010000211          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDEA010000211 DACDEA010000000
VERSION     DACDEA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.220"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV136_002877"
     CDS             <1..888
                     /locus_tag="IV136_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8466772.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDEA010000212          872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDEA010000212 DACDEA010000000
VERSION     DACDEA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.221"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            167..838
                     /locus_tag="IV136_002878"
     CDS             167..838
                     /locus_tag="IV136_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8466773.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          335 a          127 c          129 g          281 t
ORIGIN      
        1 aaataaaata ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca
       61 tgtattcttt tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga
      121 acagaattaa aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac
      181 tagtctccaa aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga
      241 acctggaaaa aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc
      301 aatacgtgaa gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag
      361 cggaaattat atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga
      421 acatttggaa cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg
      481 gattttagaa actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag
      541 tttttttcat aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga
      601 agtatggcaa tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt
      661 aaaaacggaa ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc
      721 attaacaaaa caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat
      781 gcttaatgag caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact
      841 tttaactctt tttaaattaa atggttgttc tg
//
LOCUS       DACDEA010000213          825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDEA010000213 DACDEA010000000
VERSION     DACDEA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.222"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..793
                     /locus_tag="IV136_002879"
     CDS             92..793
                     /locus_tag="IV136_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8466774.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          294 a          149 c          143 g          239 t
ORIGIN      
        1 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
       61 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      121 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      181 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      241 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      301 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      361 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      421 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      481 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      541 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      601 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      661 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      721 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      781 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaa
//
LOCUS       DACDEA010000214          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDEA010000214 DACDEA010000000
VERSION     DACDEA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.223"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            59..>810
                     /locus_tag="IV136_002880"
     CDS             59..>810
                     /locus_tag="IV136_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8466775.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFS"
BASE COUNT          312 a          110 c          132 g          256 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc
//
LOCUS       DACDEA010000215          797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDEA010000215 DACDEA010000000
VERSION     DACDEA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.224"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IV136_002881"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IV136_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8466776.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          294 a          123 c          149 g          231 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaag
//
LOCUS       DACDEA010000216          779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDEA010000216 DACDEA010000000
VERSION     DACDEA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.225"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..695
                     /locus_tag="IV136_002882"
     CDS             57..695
                     /locus_tag="IV136_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8466777.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          241 a          128 c          126 g          284 t
ORIGIN      
        1 aactcttcat gtatactgac tctatcttca aaaactgaat agtaggagtt attatcatga
       61 ataacatttt aggaaaaaaa atagaactta aaaaagggat attgggcact attgtcgttg
      121 tactcggttt gttagggttg ctatttgcgc catccattat tagcattttt tctttaacta
      181 tcactggatt tatagcattt acacttgttt ttggtaaaaa agatacgaaa aaaatgttct
      241 cgaaacctgt cgctcctgtc aaaaatatag ccaaatattt ttttatcaat gtagtaatca
      301 gtgccgcagt ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca ggcaatccaa
      361 taaatgaggc tttcagcccg ttattattta ttattttgcc tatcatgata cttggagaag
      421 aattgttctc agtgtatttt ctcgctattt tttctagtaa gttcagcatt cctgtcgcta
      481 gtttcttgtc agcaatcatc tttggattta tccattacag tacttatgat aatggaaata
      541 ttttacacac tgtagcacac atccttttga tacaaggggt ggcaagattg ttattcaacc
      601 aagctgccat taaaagcaac tcgatcatta caagttgggc agttcatgtg atatttgatt
      661 tcacagctat attgctagtc gttttattct cctaaatttt tatagaaata atacatgctc
      721 tattgctaag aaagcatact ttatttcctt taaacaaaaa gacagatgta tcaatggat
//
LOCUS       DACDEA010000217          767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDEA010000217 DACDEA010000000
VERSION     DACDEA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.226"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..405)
                     /locus_tag="IV136_002883"
     CDS             complement(<1..405)
                     /locus_tag="IV136_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8466778.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQ"
     gene            complement(410..595)
                     /locus_tag="IV136_002884"
     CDS             complement(410..595)
                     /locus_tag="IV136_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8466779.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          234 a          151 c           92 g          290 t
ORIGIN      
        1 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
       61 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
      121 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
      181 atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt gtgaatcaga
      241 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
      301 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
      361 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
      421 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
      481 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
      541 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
      601 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
      661 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
      721 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcg
//
LOCUS       DACDEA010000218          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDEA010000218 DACDEA010000000
VERSION     DACDEA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.227"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(133..717)
                     /locus_tag="IV136_002885"
     CDS             complement(133..717)
                     /locus_tag="IV136_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8466780.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          249 a          170 c          103 g          243 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catat
//
LOCUS       DACDEA010000219          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDEA010000219 DACDEA010000000
VERSION     DACDEA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.228"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..>764
                     /locus_tag="IV136_002886"
     CDS             19..>764
                     /locus_tag="IV136_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8466781.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          276 a          141 c          133 g          214 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      481 gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg tcaaaccgta
      541 tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa agaagtactg
      601 agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct acaggatatt
      661 aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa aggcatgaaa
      721 gatactatcc atcaaattta tcctaaagca aaatatcagc attg
//
LOCUS       DACDEA010000220          761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDEA010000220 DACDEA010000000
VERSION     DACDEA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.229"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(593..736)
                     /locus_tag="IV136_002887"
     CDS             complement(593..736)
                     /locus_tag="IV136_002887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466782.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            655..>761
                     /locus_tag="IV136_002888"
     rRNA            655..>761
                     /locus_tag="IV136_002888"
                     /product="16S ribosomal RNA"
BASE COUNT          313 a          124 c          134 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag a
//
LOCUS       DACDEA010000221          729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDEA010000221 DACDEA010000000
VERSION     DACDEA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.231"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IV136_002889"
     CDS             7..624
                     /locus_tag="IV136_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466783.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          274 a           93 c          135 g          227 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaa
//
LOCUS       DACDEA010000222          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDEA010000222 DACDEA010000000
VERSION     DACDEA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.232"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..448)
                     /locus_tag="IV136_002890"
     CDS             complement(<1..448)
                     /locus_tag="IV136_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8466784.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          217 a          126 c           95 g          280 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatcc
//
LOCUS       DACDEA010000223          640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDEA010000223 DACDEA010000000
VERSION     DACDEA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.234"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..250
                     /locus_tag="IV136_002891"
     CDS             <1..250
                     /locus_tag="IV136_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HAP8466785.1"
                     /translation="GIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQ
                     DIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(380..>640)
                     /locus_tag="IV136_002892"
     CDS             complement(380..>640)
                     /locus_tag="IV136_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8466786.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          241 a           67 c          115 g          217 t
ORIGIN      
        1 tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg aagaagaaat
       61 aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata ttgagaaagc
      121 aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg tttatggaat
      181 taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata aaaggactta
      241 taaagattga ttatataata tatgaaaagc tattataaaa gacattagta ttaaatagtt
      301 taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg taaaatgcta
      361 tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa gaataaatcc
      421 attcatcatg taggtccata agaacggctc caattaagcg attggctgat gtttgattgg
      481 ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt tcaattagat
      541 tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag gcgtcttcga
      601 atccatcatc caatgatgcg caagcttttg aatattttgg
//
LOCUS       DACDEA010000224          639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDEA010000224 DACDEA010000000
VERSION     DACDEA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.235"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..>639
                     /locus_tag="IV136_002893"
     CDS             12..>639
                     /locus_tag="IV136_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8466787.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          225 a          113 c          109 g          192 t
ORIGIN      
        1 gaggactaca tatgcgttca tcaaaatata ctgaacatta tacagatact tttataacat
       61 tcaaggaaat tatgagaact gtttctaaca tataccacaa ttgtgtacca gataagatta
      121 aaaaccgaag aaatactgat caactgaagc aacgcgatac agtgattatt gcttgtgtta
      181 tatggggtat aattaatggc tatactagcc aaagagccac gtatagagcg gtttgttccg
      241 tcttatttcc taatggtgac tttcctagta ggagtcgatt cactcgctta agttcaaact
      301 tagcttacac tatcaagatt attcgatact ttttcatcaa gaagctcacc aaaggtgaac
      361 tagtcggaat tatagatagt tttcctagtc cattatgtaa accagttaga aatagacaag
      421 caaaactatt gaatcaaata gccaaagttg gctataactc aacaaaaaaa tcttattttt
      481 atggtcttaa aattcatatg atcgttacta agactggctt tccgattact tactcaatca
      541 cgaatccagg tgtccatgac gtgaaagtgt tagagacttt atcggaagaa gcgaatcttc
      601 ccaatatcct aggggacaaa ggatatatta gccataaaa
//
LOCUS       DACDEA010000225          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDEA010000225 DACDEA010000000
VERSION     DACDEA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.236"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>618
                     /locus_tag="IV136_002894"
     CDS             <1..>618
                     /locus_tag="IV136_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466788.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          223 a          100 c          123 g          172 t
ORIGIN      
        1 tccggtaaat cattagaagc attagaactc gaacaagagg aaatcagaag atttcagaat
       61 tgctccttgt accctgttta tcatggaagc gcaaaaagca acatagggat tgagcagctt
      121 atagaagtga taacgaataa attttattca tcaacataca gaaagaagtc tgaactttgc
      181 ggaaatgtct tcaaaattga atattcggaa gaaagacaac gtcttgcata tgtacgcctt
      241 tatggcggaa tcctgcattt gcgggattcg gttagaatat cggaaaagga aaaaataaaa
      301 attacagaaa tgtatacttc aataaatggt gaattatgta aaattgataa ggcttattcc
      361 ggggaaattg ttattttgca aaatgagttt ttgaagctaa atagtgttct tggagataca
      421 aagctattgc cacagagaga gagaattgaa aatccgctcc ctctgctgca aacaactgtt
      481 gaaccgagca aacctcaaca aagggaaatg ttacttgatg cacttttaga aatctccgac
      541 agtgacccgc ttctacaata ttatgtggat tctacgacac atgaaattat actttctttc
      601 ttagggaaag tacaaatg
//
LOCUS       DACDEA010000226          603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDEA010000226 DACDEA010000000
VERSION     DACDEA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.237"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            142..510
                     /locus_tag="IV136_002895"
     CDS             142..510
                     /locus_tag="IV136_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8466789.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          210 a          104 c           90 g          199 t
ORIGIN      
        1 ctcttttcta ttttattatt tgagacatct tatcatataa atatagcggt tacaacatta
       61 cttgtgtttt gttcgttttt tatacataac tattgttttt ctggtaaaat aaatcaaacc
      121 acttaatgga ggcgttttag aatggaaaaa tttcagccga tcatcaatca aatcaaagaa
      181 aaagaacatc aacaaaagat caaagacctt tttgctaaga taacagaaac gtatccccag
      241 ctagacacaa ctgtaaaatg gaaccaacct atgtttactg accatggtac ctttattatt
      301 gcatttagtt ttgctaaaaa tcatttttct atcgcaccgg aaaaagcagc gattcgtgcc
      361 cttgaaaaaa atatccaaga agcaggctac gtatatacgg ataacgtcat caaagttcct
      421 tggaaaagtg taatcaactg ggaattgatt gaacaattga tttcatttaa tattgaagat
      481 aaaaaaggtc atgataaatt ttggcgataa agctttatac gaataaacct tttactggcg
      541 aggaaactat tcatcacctt atacaggttt tgttcaatcc tatccttttt atcatccctg
      601 gtt
//
LOCUS       DACDEA010000227          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDEA010000227 DACDEA010000000
VERSION     DACDEA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.238"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IV136_002896"
                     /pseudo
     CDS             79..234
                     /locus_tag="IV136_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>595
                     /locus_tag="IV136_002897"
     CDS             253..>595
                     /locus_tag="IV136_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8466790.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFK"
BASE COUNT          247 a           56 c          104 g          188 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taaga
//
LOCUS       DACDEA010000228          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDEA010000228 DACDEA010000000
VERSION     DACDEA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.239"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(318..581)
                     /locus_tag="IV136_002898"
     CDS             complement(318..581)
                     /locus_tag="IV136_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP8466791.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACDEA010000229          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDEA010000229 DACDEA010000000
VERSION     DACDEA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.241"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..269
                     /locus_tag="IV136_002899"
                     /pseudo
     CDS             1..269
                     /locus_tag="IV136_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            437..>543
                     /locus_tag="IV136_002900"
     CDS             437..>543
                     /locus_tag="IV136_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466792.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSR"
BASE COUNT          200 a           84 c           94 g          165 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaattaatc aatctgttgc taaaactgcc cattgcttga atcgttcaag
      541 aca
//
LOCUS       DACDEA010000230          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDEA010000230 DACDEA010000000
VERSION     DACDEA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.242"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..>536
                     /locus_tag="IV136_002901"
     CDS             51..>536
                     /locus_tag="IV136_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8466793.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DACDEA010000231          532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDEA010000231 DACDEA010000000
VERSION     DACDEA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.243"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..445)
                     /locus_tag="IV136_002902"
     CDS             complement(<1..445)
                     /locus_tag="IV136_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466794.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKG"
     gene            complement(462..>532)
                     /locus_tag="IV136_002903"
     CDS             complement(462..>532)
                     /locus_tag="IV136_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8466795.1"
                     /translation="TTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          149 a           90 c           84 g          209 t
ORIGIN      
        1 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
       61 tgaatttact atcaatcaaa gcaattgtac cattttgatt attcgttgta taactctgaa
      121 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
      181 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
      241 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
      301 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
      361 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
      421 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
      481 tgattttgga tatacttctt aatcacatca attggtgcgc caccagttgt ca
//
LOCUS       DACDEA010000232          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDEA010000232 DACDEA010000000
VERSION     DACDEA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.244"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          183 a           97 c           68 g          183 t
ORIGIN      
        1 aattcgcttg tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt
       61 aatcaaacag actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc
      121 tacctatttt tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat
      181 tggttaaatc tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca
      241 tgtcatacaa attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg
      301 agcagttact tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt
      361 ctttgttgca attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct
      421 ccaataaaaa ctatctacct ctcaaattat ttttctctaa taagaaaaac agaaaaggta
      481 ataagataca acagtgatga ttcctaataa agaaaagact acaataatat t
//
LOCUS       DACDEA010000233          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDEA010000233 DACDEA010000000
VERSION     DACDEA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.245"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          186 a           76 c           89 g          169 t
ORIGIN      
        1 atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat
       61 aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga
      121 ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa
      181 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
      241 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
      301 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
      361 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
      421 catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt catcctaaca
      481 taaaataatt gttcactcaa aagtttttct agaacaatta
//
LOCUS       DACDEA010000234          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDEA010000234 DACDEA010000000
VERSION     DACDEA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.246"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>512
                     /locus_tag="IV136_002904"
     CDS             <1..>512
                     /locus_tag="IV136_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466796.1"
                     /translation="LVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFK
                     KGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGL
                     KHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFS
                     NWKQISNVND"
BASE COUNT          171 a           87 c          104 g          150 t
ORIGIN      
        1 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
       61 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      121 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
      181 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
      241 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
      301 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
      361 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
      421 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
      481 caaattggaa acagatcagt aatgtcaatg at
//
LOCUS       DACDEA010000235          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDEA010000235 DACDEA010000000
VERSION     DACDEA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.247"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..469)
                     /locus_tag="IV136_002905"
     CDS             complement(<1..469)
                     /locus_tag="IV136_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8466797.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSL"
BASE COUNT          178 a           93 c           66 g          156 t
ORIGIN      
        1 ttaagctcct aaactcttca atacgaacta cataataacc agtagaacgg tactgcataa
       61 gcagtcggaa tagagttttt gcatacgtag aacgcaacga cgtgaaagca gataattcaa
      121 atctactaaa ttcagctgtt aaaccattaa taatatattc taggtccgga ttaacactga
      181 cttcaacaaa cttttttgtt ttattgattt caaaaccagt aaacaaaaca aagtagcgaa
      241 tagaatcctc atcttcagta cggtaagtaa gattaagcat atctttatag agcttttcta
      301 aatcaccgac aaaggctttt gtagcagtca tcttataatc acttaactct tttaattcct
      361 cgaaagaaaa tctaactttt ttcaaaccac gatctttcat acgagcgcat aatgcaaaaa
      421 ataaatccat ctgcttagca ttgaaatttt tcagcggtac taaattcaat tcattttgat
      481 atttgactgt taa
//
LOCUS       DACDEA010000236          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDEA010000236 DACDEA010000000
VERSION     DACDEA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.249"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>482)
                     /locus_tag="IV136_002906"
     CDS             complement(<1..>482)
                     /locus_tag="IV136_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8466798.1"
                     /translation="FSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQ
                     HSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNEL
                     KRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFI
                     "
BASE COUNT          176 a           85 c           60 g          161 t
ORIGIN      
        1 gtataaaaat taaaacaata ccaagaaacg cataattcac tttacttcta aaaaatgttt
       61 tgagtaaact aaagatattt aacacataga aagcatagat acatataaaa gcaattatac
      121 cacttgatac aagtacttga agaaatccat tatgcatgtt tccatgtgct ctttttagtt
      181 cattcacatc tgtagtatca agatctactt ttttcaaaaa ttttgcatcg ctattcccat
      241 agaaatcggt ctctccataa ccaaataaca aagttggtct tataacttta agcgcagctt
      301 tccaaatcgt aaaacgacca gctgaaattt cggagttatc actagaatgc tgtaatatta
      361 cttctttttc tggaagtgga gttttatctg attcatttga ctctataact tctacttttc
      421 tatcaatcga tacaggaatt gttgctaaaa aatccttagc ataattagta ccaaaagaaa
      481 aa
//
LOCUS       DACDEA010000237          476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDEA010000237 DACDEA010000000
VERSION     DACDEA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.250"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..>476
                     /locus_tag="IV136_002907"
     CDS             22..>476
                     /locus_tag="IV136_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8466799.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DACDEA010000238          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDEA010000238 DACDEA010000000
VERSION     DACDEA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.251"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            237..>461
                     /locus_tag="IV136_002908"
     CDS             237..>461
                     /locus_tag="IV136_002908"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8466800.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLF"
BASE COUNT          157 a           86 c           59 g          159 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt t
//
LOCUS       DACDEA010000239          411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDEA010000239 DACDEA010000000
VERSION     DACDEA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.253"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>411
                     /locus_tag="IV136_002909"
     rRNA            <1..>411
                     /locus_tag="IV136_002909"
                     /product="16S ribosomal RNA"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DACDEA010000240          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDEA010000240 DACDEA010000000
VERSION     DACDEA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.254"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>408
                     /locus_tag="IV136_002910"
     CDS             <1..>408
                     /locus_tag="IV136_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466801.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGE"
BASE COUNT          168 a           61 c           93 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaa
//
LOCUS       DACDEA010000241          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDEA010000241 DACDEA010000000
VERSION     DACDEA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.255"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV136_002911"
     CDS             <1..390
                     /locus_tag="IV136_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8466802.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          155 a           72 c           60 g          107 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaa
//
LOCUS       DACDEA010000242          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDEA010000242 DACDEA010000000
VERSION     DACDEA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.256"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IV136_002912"
     CDS             <1..285
                     /locus_tag="IV136_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8466803.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACDEA010000243          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDEA010000243 DACDEA010000000
VERSION     DACDEA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.denovo.257"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>362)
                     /locus_tag="IV136_002913"
     rRNA            complement(<1..>362)
                     /locus_tag="IV136_002913"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DACDEA010000244        12501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.070, whole genome shotgun sequence.
ACCESSION   DACDEA010000244 DACDEA010000000
VERSION     DACDEA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.070"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1185)
                     /locus_tag="IV136_002914"
     CDS             complement(<1..1185)
                     /locus_tag="IV136_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8466804.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKG"
     gene            1467..2363
                     /locus_tag="IV136_002915"
     CDS             1467..2363
                     /locus_tag="IV136_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8466805.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(2506..3447)
                     /locus_tag="IV136_002916"
     CDS             complement(2506..3447)
                     /locus_tag="IV136_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8466806.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3573..4694
                     /locus_tag="IV136_002917"
     CDS             3573..4694
                     /locus_tag="IV136_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8466807.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            4952..6454
                     /gene="lsa"
                     /locus_tag="IV136_002918"
     CDS             4952..6454
                     /gene="lsa"
                     /locus_tag="IV136_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8466808.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            6521..7048
                     /locus_tag="IV136_002919"
     CDS             6521..7048
                     /locus_tag="IV136_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8466809.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            7260..8321
                     /locus_tag="IV136_002920"
     CDS             7260..8321
                     /locus_tag="IV136_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8466810.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8324..9220
                     /gene="murQ"
                     /locus_tag="IV136_002921"
     CDS             8324..9220
                     /gene="murQ"
                     /locus_tag="IV136_002921"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8466811.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            9234..11216
                     /locus_tag="IV136_002922"
     CDS             9234..11216
                     /locus_tag="IV136_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8466812.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11286..11732)
                     /gene="nrdI"
                     /locus_tag="IV136_002923"
     CDS             complement(11286..11732)
                     /gene="nrdI"
                     /locus_tag="IV136_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8466813.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            11943..12464
                     /locus_tag="IV136_002924"
                     /pseudo
     CDS             11943..12464
                     /locus_tag="IV136_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         4091 a         2246 c         2678 g         3486 t
ORIGIN      
        1 tcctttgtcc cataacgttc ctttccttag tgtcagtaca aaggacgcag cactgataat
       61 gatccagatc aataatactg tataggaaag agaacctgcc agatagccaa ataatttgtc
      121 accgacaaaa taagaaagag ccacacctag ataaagaaca ctggtacaaa acagcacagc
      181 acgttgcggt acttgatgct tgtttaaaac agctagtttt ctagaggaac ctttccgatc
      241 tttcaagcgg aagaacaaaa ttctcgaaga agcatataca cctgaattga tagacgagaa
      301 cagagcaaga acaatcacga aattgacgat atctgccgca aatggaatat tcattttttc
      361 aaataccatc acaaaaggac taacgttcgt gcctgctaaa gtattccaag gaaaaataat
      421 caaaagcaaa aataaaggaa taatgtaaaa ggaaatgatt cttcccatga cgcctttgat
      481 tgctttagga atcgcacgtt taggatcatc tgcttcgctt actgtgatag caatcaactc
      541 agaaccgccg taagaataga taactacaag caaagaactg atgattccct tcataccgtg
      601 gggagtaaaa ccaccatgat cagttaattg ttgaagggta gggaaaacac cagtacccaa
      661 catttgctga ccgacaagat aagcagcaaa aatgatcaat aggataatga cactgatttt
      721 agcaaaggcc agccaatatt ctgtctctgc gaataatcgt accgaataaa gattgatgat
      781 agtagttaaa acagccaaca taaaaacaaa tacccaagct gggatgtggg ggaaccacaa
      841 ctgtaaaaag gaagcagctg caacagcttc agcaatgata ttgataatcc acatggacca
      901 gtagacccaa tcggtaaaat cggcagcatg agcacctaga tatggacgaa tcaatccaga
      961 taatccgtgg tgcgtatcac caccacttaa aacaattttt cccagacctt tcatcacgat
     1021 atataaaatt atgccgccaa tcaaaaaagc gatcaataca gaagggccgg cgattccaat
     1081 cgcttcgcca cttcctttaa atagacctgc accaatcgct ccacctaaag ctagcatggt
     1141 gatatgtcgg gagctaagtt gttttttcaa ttttttatct tccataaata acctctttct
     1201 tttaaattca caatcaaaat gcatagtaag aataaatagt aagaataaat agtaataata
     1261 ataatgacta atgataacaa attttcagat aattacaact attcagaaaa cttttgggca
     1321 tattttaatg gaagaatgat ttatttcatc gtattttcat agaaatgtta aatttttata
     1381 aaaataatag gaagcgtttt actggctttt tactatttaa acaggctatc ataggaaaaa
     1441 gtgacagaga aagtgaggcg gaacagatga acaaaaacga cgtattgcta attgcgaaaa
     1501 ataaatcact ttattttttt ccttccatcc gctctttaga tgtttggtcc ggaaaagatt
     1561 tactacagtc ggaattcatt aaatcagctc gacaacagtt ggcaaattat cagctgatcg
     1621 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgtcca tttaccaatg
     1681 caaaaatcgt gttatttttt tggaaccact ttaaagcaga acacgaggcg atgctgaagc
     1741 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgatttttta gaagcgaaag
     1801 aacttggact aaaacataat agttcctttt attcgaaaca tatggatttt tcttctagcg
     1861 aaccaaagac ggatctattt tttggtgcaa caaataacgg cagaaaagaa cgggcagaaa
     1921 aatataagca ggaatttaca aaaagaggtg ttacgaccaa ttactttatt cttccgcttc
     1981 gaggaaatga acaacctgga taccttactt atgaagaata tctgaaaaaa accggggaat
     2041 cgagggggat tctagaactg ttgcgggaag ggcagcaagg agtgactttg cgaacgtttg
     2101 aatctatgta ttttcaaaag aaactagtga cagataatca agccatagct cattatcagt
     2161 tttatcattc tgaaaatatt tttttgcttc aagaacgtga tttagatgaa ttaccggaat
     2221 ttttgcatac gccatttcaa ccaatcccat cagaactgtt ggaatttttt gatgcagaaa
     2281 attgggcaaa acgttttttc actactgact ccgctattta tgaacaatat gaatacgatc
     2341 tttcctctgt taaaaataaa taaaagtgcc aaccaatcat agatgaaaat aaacaagcgc
     2401 aattcttatg gcaacatgag aattgtgttt ttctttttga actttcatgg gggtaaaaaa
     2461 atgagaacca gctgctgatc cagctgattc tcatcagaga gccttttatt ctgcgccaag
     2521 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
     2581 atatttttgt ccttttcgcc aaacggcacc attgatgtag tcgatttcag tcaaacgatg
     2641 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
     2701 agtagaagca atatgttggt acacttcttc ttgatctaat gtgattcctt cttttgctgc
     2761 gatagaagca aactcagata caatcgttcg aaccatgccc tctgccgctt tttgcgcacc
     2821 taattcagca atattacaat ccaatatcgt gcataatcca tttaatgttc cgtttacaca
     2881 agcttttcgc caaatggaat aacgaacatt atgactatag attggattca attttgcttc
     2941 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     3001 ttcgatttct ccatcaccta ataatgtgac ttttccggga ccagctagac cagctgtcca
     3061 catcgtgatc ccaaataaga tatttttctt tggtacatat ttttccagta catcttcatg
     3121 acctaatcca ttcagtaaac acaaaacata agtattctca gtaataatgg attggatcga
     3181 acgaaacata tcatctaatt gattcgcttt tgtcaaggct acgatcaaat ccacgtgttt
     3241 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcaacta cttctttgcc
     3301 attaaagtca gcaatcaatc catttgtacg aatggcttca atgtgagcag gccagcgatc
     3361 gatcaagacg acctcatttc cgctttgatg caacattaat ccgattcggc tgcccattgc
     3421 tccggctcca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     3481 ttagtatata gaaaaacaga agaaaatagc attgttttgt ttgaaaaaag tgaaaaaaag
     3541 tgtcttactt aagaagaaag tgagggatat agatggatat tttggcggca atcgattcat
     3601 ttaaaggaag tgcttcttca tttgaattaa atcaggcagt tctaaatggt tgggaagacc
     3661 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     3721 tctattacgc gctcggggga acctggcgta cgattgatgg gatggatctc ttatttcgtc
     3781 agcgaaaaat cagttattta ttgacgaact ggcaaggaaa aaataccgca gtcattgaat
     3841 ctgcacaagt attaggatta gatttacttg ataaaccaac ggatcaaacc atccgacttg
     3901 cttctagtta tgccttggga gagcttatca aagatacact gcttcaagaa gtggaacaaa
     3961 tcattgtcac tttaggagga agcggatgta gcgatggtgg tctaggatta ctccaatctt
     4021 tgggtgcttc tttagaaggg tacacagaag gaaatccttt gttatctact agaaaacttc
     4081 atttggatcg aaggaacgtt cttgtttcta aagtaaaagt gatcatcgct gcagacgtga
     4141 ccaacccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     4201 cggatacgac gttagctttc ttggataaac aagctgaaaa gattgctcag cagataaaag
     4261 atgtttggga ggtggattta gatgacatcc caggaagcgg agcagcaggc ggtattggcg
     4321 gagccctttt tttgttagga gcgaagatgg tctctggttt tgagttggtc agccaaatca
     4381 ctggattaga aaaacagatc gaacaagcgg atgtcatcat tacaggagaa gggcggattg
     4441 atcagcaaac aatccacgga aaagttccat atggcgtagc agtgcttgca aaaaaatatc
     4501 gaaaaccaat aatcgctatt tgcggaagta gagaccgaga gctggaagga ctctttgatt
     4561 ttctccccat tgtcttaagc attcaattag gaccaatcga tttagaggaa gcaatggaac
     4621 acaggcaaac attagaaaaa acagaaatct taggacagac actcagcaga tttttcaaca
     4681 ttctcccgaa gtagttgaca aaaacaattt ctttatgcta tcgtaatttc aaatatagga
     4741 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     4801 gtaccttgtg gagacaaggg agcagtgtgc agattttcgg gattggcttg gttctttcga
     4861 tgtatgggtc tttttgtgtg ccccaaccct tgcatcctct ttagccagtt ctgaaaatag
     4921 gactggtctt tttttacgag gtgatggaat aatgtctaaa atcgaaataa aaaatctgac
     4981 attcggctat gacagccaag gcacattatt atttgaacaa gccaatctaa actttgacac
     5041 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     5101 cctgcaaaac aaactccctt atcaagggca agtgatccat cagcaagcgt ttgtctattt
     5161 cccgcagcaa acaaaagata aagagcgttt gacctattat gtgttaaatg agattatgga
     5221 ttttgagata tgggaaattg aaagagagct ccaactgatg caaacagatc ctgaaatctt
     5281 atggagagaa ttcagcacac tatcaggagg agagaagaca aaagtcctac tggcactctt
     5341 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     5401 tggcagaaaa caagtagcgg cttacttgaa aaagaaaaaa caaggattca tcgtggtgag
     5461 tcatgatcgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaagagcca
     5521 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     5581 atttgaaata gctaaaaatg aaaaattgaa aaaagaagtc agcaggctaa agaaaacagc
     5641 agctgaaaaa gcagaatggt ctcgttcccg agaaggggac aaaacaaaga aaaaagtcgg
     5701 attcatcgat acggaatcta ggcgagtgaa taaaggagca gtaggcgctg atgctgcacg
     5761 gacgatgaaa cgatctaaag caatcgtgaa tcggatggaa acccagatca gtgagaaaga
     5821 aaaattatta aaagatatcg agtatatcga tgctttgacg atgaatagcc aagtgtctca
     5881 tcataaacgg ctgttaagcg tagaaaatct tcaattaggg tatgaaaatc tgttattcga
     5941 gccagttcat tttacgatcg aacctcatca gcggatggca atttcaggtc ctaacggcgc
     6001 gggaaagtca tccattatcc attatcttct ggggacattc aacggcaagg ttgtaggaga
     6061 aaaatcccag cccaaacatt tgaccattag ttacgtacgc caaaattatg aagacaatcg
     6121 agggacattg gcggaattcg cgaagaaaaa ccgagtagac tatcaagcat ttttgaataa
     6181 tctccgaaag cttgggatgg aaagagatgt ttttcataat aaaatcgaac agatgagtat
     6241 gggacaacgg aaaaaagtgg aattggctaa atctttatcc caaccagctg agctctatat
     6301 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     6361 tttgaacgtg aaacctgcta tgttactagt ggaacatgat caaacctttc tagataaagt
     6421 atctactgag attatttctc ttgagaaaat ctaaaccttg taatctcaaa gacaacaagg
     6481 tatggttaag gagaactttg tcaacgaagg agagaatcac atgctttatg taacgagaca
     6541 cggagaaaca acttggaacg cacaaggatt agtttgcggg cgagcagatg tgccgctgac
     6601 tgaaaaagga cagatgcagg cacaggcact agcagaaaag gtagtagatt tgcctgttcc
     6661 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagtagt
     6721 tgatcgtctc aatttgccga tgaccgctga tgagcgattg gtagaaatgg attttgggga
     6781 ttatgacgga ctgccaagca aagatgaaaa ctttcaaaaa gcgcgtttag cgtttgctgt
     6841 acgtttccca aatggagagt cggtcttaga tgtgtacgca cggatcgttc ctttattgaa
     6901 agaatgcatg gaagacgaag aaaatgtcta cttgttagtc tgtcataatg ccttgataag
     6961 agtgatcaat gcttattttc atccaatgcc taatgaaggg ttctttacat tcatggtaga
     7021 caatacagaa ttaatttctt atgagtaatt agacttttgt gttatttaaa taaaatgttt
     7081 caaggcactg cttttgctta gcagtgcctt ttttgtggct gtgtcaattc ttttggcatt
     7141 ataaatccaa tttttagaaa taaaatttaa ttattttaac aaaagtattg aaattttgtt
     7201 tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag agggataata
     7261 tgtttggctt ttcaatattt atgaatgatt ctttgtcaga agagaagaaa cagtatatcc
     7321 gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata ccggaagatg
     7381 atgtttcggc ttatcaaaat cgattgatcg aactaggaag cgaagcgaaa aagaataaac
     7441 tagagttgat ggtcgatatt tcaggagagg cattgaaacg agctggcttt tcattagaag
     7501 atctaacacc cttgaaagca attggtgtaa cagggttaag aatggattat catatttcta
     7561 atcagcagat cgccgactgg tcacatcaat tgaaaatcag tttgaatgcc agcacgatca
     7621 cgcctaatga tatcgatgag ttgaaagaag cagcggctga tttctcacag attgaagctt
     7681 ggcataatta ttatccacgt cttgaaacag ggttggacaa agaatggtat caggaaaaaa
     7741 atcaatggct caaaactcaa ggattccttg tacaaggctt tgtaccagga gatacagagt
     7801 tgagaggtcc actgtatcaa ggattaccaa ctttggaaga acatcgaggg atccatcctc
     7861 tggcaggtgc tcttgatcta ttagcaagta acacggatat tgtttatatc ggagatgcag
     7921 gtctgtcaga acacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc
     7981 tccatacgga acctgtagat gaagtttttt acgaatatat attaggaaaa catacgaatc
     8041 ggcaagatga tgcaagagat gtcatacgaa gcgcagatgc gcgttttcga gagattccgc
     8101 caatcccagt aagaaataca gctaagcgta tgaaaggatc tattacttta gataacgaaa
     8161 agtatttgcg ctatatgggt gagatccaat taactaaata tgatttgcca gcggatgaaa
     8221 aagtgaatgt agtagccaaa gtcatcaaag aagaactacc gctgatcaac caaatcaaag
     8281 ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt
     8341 agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg aaatgagtat
     8401 ttctcaagca gtccaactca tgaacaaaga agatcagaaa gtggccgctg ctgttgaaaa
     8461 agaactgcca agtatcgagc cagtgatcgc caacacgatt gaatccttta aaaaaggtgg
     8521 tcgcttgatt tatatgggag ctggaacgag tggtagactt ggtgtattag atgctgctga
     8581 atgtgtaccg acattcggtg tagaaccgga aatggtcata ggcctaattg ctggtggaga
     8641 agaagcaatg actgtagccg tcgaaggagc cgaagattct tatgaattag ggcaagaaga
     8701 tctaatcaag ctgaatttaa caaaaaatga tatagttatt ggtatcgcag ctagcggtcg
     8761 tacaccatat gtgattggtg gattagatta cgcaaaatcg attggtgctg ttacaggaac
     8821 gatttcttgc aataaacaag cagaaatcag cgagcatgcg gatttcccaa tcgaagtcga
     8881 tgcaggacca gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact
     8941 gattttgaat atgatttcta ctatcagtat gatcggtatc ggtaaagtat tcaataactt
     9001 gatggtcgac gtcaaaccaa caaatgaaaa actaattgaa cgctcaaaac gaatcatcat
     9061 gcaagcgaca gaagctgact acgaaacagc tgaaaagtac tttgaggaag cagatcaaaa
     9121 tgtcaaactg gctatcgtca tgattttgac aaatagtgat aaagaggcag ctcaagcaaa
     9181 attagctgaa gcaaacggtt ttgtaaaagg aaccttatga gggggaaaag accatggcaa
     9241 aaaacgaatt gactttggaa gaactggcaa aacaaattta tcaaggagca ggcggtatgg
     9301 gcaatgttga aagcattgtc cactgcatga ctcgtgtacg tatgaaggtc atcaatgatg
     9361 atcttgtaaa tgtctcggaa ttaaaagcga ttccaggagt acttggcgta gtagaggatg
     9421 aacagctcca agtcatcatc ggacctggga aagtgaacaa agtagcgcag gaaatggttg
     9481 acttagcagg cgtgaaatta ggagaaaaac ttccttctgc tagtgcaact acagcaagtt
     9541 cttctgcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa
     9601 aatcgaaaca aaaaccctct aaaatcaaat cggtattaaa agacatctct aatatctttg
     9661 taccaatgat ccctgcattt gtcggcactg ggatcgtggc agggattgcc gcagttttag
     9721 caaacttagt cacagctgga acactagacg caggaacatg gcaacaatat atcgatgtga
     9781 tgaacatctt gaaaaacggg atgtttagct atctagttat ctatacaggt atcaatgctg
     9841 cacaagtttt tggtgcaacg ccaacgcttg gtggagttat cggggctgtt gtgatgctga
     9901 cagggatgaa tccagaagca ccactgaaaa acttgtttac cggtgaagct cttgcaactg
     9961 gacaaggcgg gatcttaggt gttatcttcg cagtttggtt attgtcaatc gttgagaaaa
    10021 aactgcataa agtagtacct gatgcggtag atatcatcgt tacgccaact ctttccctga
    10081 ttgccatcgg tctgatcgaa attttcttca tcatgccttt agcagggttc atttctgacg
    10141 gcatggtcgg tgggatcaac tgggtacttg gtgttggagg agcatttgcc ggcttcgttc
    10201 taggtacatt attcttgcca atggtcatgt tcggactaca tcagatcttg acgccaatcc
    10261 atgtacaaat gattgatgaa actggtcgta ccttattgct tccaatcctt gcgatggccg
    10321 gagcaggaca agtgggtgca gcattggcat tatggatccg ctgcaaaaaa gacaaagaat
    10381 taacagaaat gatcaaaggg gctttaccag tcggaatctt aggaatcggc gaaccattga
    10441 tttacggagt aacattacct ctaggacgtc catttattac tgcttgtatc ggtggaggta
    10501 tcggtggggc agtgatcgga atgatcggaa atgtcggagc aatcgctatt ggaccttcag
    10561 gagccgcttt gattccattg atctctgatg gtaaatggtt aggctatatc cttggtttat
    10621 tggctgctta cgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt
    10681 tagaaaaaga agaactagcg accgaaacag ccacagtaaa tgaagtacct gaaaaagcag
    10741 tacctcaagc ttcagctgaa agtgtgacta tcttatcatc agttgctgac ggaacagttg
    10801 tttctttaga taaagcaagt gacccagttt ttgcacaaaa aatgatgggt gaaggttact
    10861 ttgtagaacc agaaaacgga catatctatt ccccggttac tggagtaata agttctgttt
    10921 tcccaacaaa acatgctatc ggcattacga caccaaatgg cttagaaatc ttgcttcaca
    10981 tgggcatcaa cacagttgac ttaggcggta cgccattcga tgtgaaagta acagaaggac
    11041 agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcaatc aaagcagcag
    11101 acaaagaaac ctccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct
    11161 tggaaaaaac aggtaaagcg aaagcaaaag cacctgtgat ggatgtcaaa gcttaataag
    11221 aatagaaaca atagagaaca taaaaagaag aagccgaaat ttatttttca gcttcttctt
    11281 ttgtgttatt tatttttttc tgccaaaatt tgataaacgc gtgtcacatc agatggtgtt
    11341 ccccgtaatt cataatctgc caagaaagga aagccgaatc tttgtgcgta ttgtttggct
    11401 gtcagacaat attgattgtt gaagttttta ttaccgctac cgactacacc aaggcaaagt
    11461 ttatgattgt cttcgtattc cagatattcg cgcatcgttt cggtcaagat ttctgtgtca
    11521 ccgttgtcta acccattccc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
    11581 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
    11641 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa agcacgcgta
    11701 tttcctgaaa tcgatatata taaaatgttc atggatcatc ccccaattct ttccctttag
    11761 tatagcatct ttttaagttt ttttggtggt gtaatttgga aagggactag gcgctttgct
    11821 tgtataatga gaaaaaagtc ggtagaatta tatttctatt aaagttttta ttaattaagt
    11881 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttactttg gaggatgctt
    11941 acatggatac agagcaacag aaaaaaggaa ttggtttgat tccacttgct gccttagtga
    12001 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    12061 gtggcccagc tatcttcagc tgggtactcg taggaacagc aatgttgatg ctctccttat
    12121 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    12181 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    12241 ggttaggaaa cgtggcgttc gcaacattgt tgatgagtgc attaggttat ttcttccccg
    12301 tattcgaagg tggtcaaaat gtggcatcga tcgtgatgag tacgatcttc ttatggggat
    12361 atgcatggct agtgaatcgt ggagttgaaa atgctagctt tgtcaatgct atcgtcacat
    12421 tttgtaaatt agtgccatta tttttattta ttgtagtagc tatgtaagcg ccccatagaa
    12481 cagtacctgt acgtcaaata a
//
LOCUS       DACDEA010000245         6891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.106, whole genome shotgun sequence.
ACCESSION   DACDEA010000245 DACDEA010000000
VERSION     DACDEA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.106"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..90
                     /locus_tag="IV136_002925"
     CDS             <1..90
                     /locus_tag="IV136_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8466814.1"
                     /translation="VKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            244..321
                     /locus_tag="IV136_002926"
                     /pseudo
     CDS             244..321
                     /locus_tag="IV136_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            566..682
                     /locus_tag="IV136_002927"
     CDS             566..682
                     /locus_tag="IV136_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8466815.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            698..2617
                     /gene="tet(M)"
                     /locus_tag="IV136_002928"
     CDS             698..2617
                     /gene="tet(M)"
                     /locus_tag="IV136_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8466816.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2736..2903
                     /locus_tag="IV136_002929"
     CDS             2736..2903
                     /locus_tag="IV136_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8466817.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2963..3316)
                     /locus_tag="IV136_002930"
     CDS             complement(2963..3316)
                     /locus_tag="IV136_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466818.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3820..4239
                     /locus_tag="IV136_002931"
     CDS             3820..4239
                     /locus_tag="IV136_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8466819.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            4265..4471
                     /locus_tag="IV136_002932"
     CDS             4265..4471
                     /locus_tag="IV136_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8466820.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(4699..4872)
                     /locus_tag="IV136_002933"
     CDS             complement(4699..4872)
                     /locus_tag="IV136_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8466821.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            4848..5513
                     /locus_tag="IV136_002934"
     CDS             4848..5513
                     /locus_tag="IV136_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8466822.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(5595..6761)
                     /gene="dnaJ"
                     /locus_tag="IV136_002935"
     CDS             complement(5595..6761)
                     /gene="dnaJ"
                     /locus_tag="IV136_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8466823.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2209 a         1221 c         1293 g         2168 t
ORIGIN      
        1 gtgaaaatga taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg
       61 aataacaatt ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa
      121 acaaaaagcc cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc
      181 tttttctttt ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta
      241 aatcaggcaa aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt
      301 aattggaaga ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg
      361 tactctcttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
      421 taagtatcta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
      481 aatttgatag tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta
      541 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct
      601 ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
      661 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
      721 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
      781 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
      841 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
      901 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
      961 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
     1021 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
     1081 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
     1141 ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa
     1201 ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa
     1261 atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt
     1321 tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga
     1381 taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga
     1441 actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat
     1501 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
     1561 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
     1621 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
     1681 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
     1741 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
     1801 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
     1861 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
     1921 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
     1981 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
     2041 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
     2101 cttaaatcaa tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg
     2161 attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag
     2221 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
     2281 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
     2341 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
     2401 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
     2461 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
     2521 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
     2581 agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa
     2641 tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa
     2701 aggaggagat agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa
     2761 cacgattaaa gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat
     2821 gcagacaaga aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag
     2881 acgcaaagac gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc
     2941 cgttatgtat ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg
     3001 ctaaagattc cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct
     3061 gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa
     3121 catgaagtaa cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt
     3181 caatattagt taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta
     3241 aacctcgttt caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat
     3301 cttctttttt acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa
     3361 acaggtatag aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact
     3421 aaacgccaat aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat
     3481 attacttcca aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa
     3541 taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac
     3601 gtccaccaat gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga
     3661 aataaatctg tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc
     3721 agtaatgcaa caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca
     3781 aggctcactt cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag
     3841 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
     3901 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
     3961 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
     4021 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
     4081 ctatcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
     4141 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
     4201 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt
     4261 tgaattgatt tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt
     4321 ttatgacggt tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa
     4381 aatcattatg caggttgatg aagaactaaa aggagaaata catacagaca tgatgaaagc
     4441 aatattgaaa tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca
     4501 cagtttcgtg ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg
     4561 taaattcgtg gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt
     4621 atgcggataa ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca
     4681 tgaagggacc gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc
     4741 ttgttttcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
     4801 cggttcattg atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt
     4861 tttcttctcc atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag
     4921 aacggatcaa tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt
     4981 tgctcactgt acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat
     5041 tgctatcatt gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa
     5101 actttggaat tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg
     5161 ccaaacaagt ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg
     5221 aagatttaga cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg
     5281 taaaacgtat tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg
     5341 catgcttcag taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata
     5401 aaaacacctt gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa
     5461 actatgacga ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac
     5521 aaaaaaacca ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt
     5581 ttttactaga aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag
     5641 aaaccgtctt cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat
     5701 gcttctcttt gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg
     5761 tcgcctgttg caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta
     5821 cctgtctgtg ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg
     5881 acttcatccc ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa
     5941 ccttcacgat caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat
     6001 ggtccgccgt tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct
     6061 acaccagctg gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga
     6121 caggttggac aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga
     6181 cggctcatca ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga
     6241 catttatgac aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga
     6301 cagatttctt cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa
     6361 gacaagtcga ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac
     6421 cgaccgcctc ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca
     6481 gagaagccgc cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct
     6541 gtgcctgcat gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca
     6601 tatgcttctg atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga
     6661 tggtattttt tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt
     6721 gagagcccta agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt
     6781 tctcatcatt ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata
     6841 cttcaaacta tttttcaaaa gaaaaagcca aagtagcaac tttggccttc t
//
LOCUS       DACDEA010000246         4929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.122, whole genome shotgun sequence.
ACCESSION   DACDEA010000246 DACDEA010000000
VERSION     DACDEA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.122"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1170
                     /locus_tag="IV136_002936"
     CDS             <1..1170
                     /locus_tag="IV136_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8466824.1"
                     /translation="GKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSG
                     RVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMY
                     NGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENG
                     TKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVIT
                     GIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFIS
                     DHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASI
                     SLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1400..2467
                     /gene="rlmN"
                     /locus_tag="IV136_002937"
     CDS             1400..2467
                     /gene="rlmN"
                     /locus_tag="IV136_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8466825.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2660..3763)
                     /locus_tag="IV136_002938"
     CDS             complement(2660..3763)
                     /locus_tag="IV136_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8466826.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4316..4864
                     /gene="aac(6')"
                     /locus_tag="IV136_002939"
     CDS             4316..4864
                     /gene="aac(6')"
                     /locus_tag="IV136_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8466827.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1627 a          851 c         1040 g         1411 t
ORIGIN      
        1 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
       61 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
      121 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
      181 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
      241 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
      301 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
      361 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
      421 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
      481 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
      541 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
      601 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
      661 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
      721 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
      781 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
      841 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
      901 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
      961 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
     1021 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
     1081 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
     1141 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
     1201 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
     1261 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
     1321 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
     1381 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
     1441 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
     1501 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
     1561 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
     1621 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
     1681 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
     1741 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
     1801 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
     1861 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
     1921 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
     1981 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
     2041 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
     2101 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
     2161 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
     2221 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
     2281 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
     2341 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
     2401 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
     2461 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
     2521 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
     2581 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
     2641 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
     2701 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
     2761 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
     2821 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
     2881 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
     2941 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
     3001 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
     3061 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
     3121 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
     3181 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
     3241 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
     3301 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
     3361 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
     3421 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
     3481 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
     3541 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
     3601 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
     3661 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
     3721 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
     3781 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
     3841 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
     3901 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
     3961 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
     4021 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
     4081 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
     4141 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
     4201 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
     4261 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
     4321 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
     4381 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
     4441 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
     4501 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
     4561 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
     4621 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
     4681 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
     4741 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
     4801 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
     4861 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
     4921 aaattactt
//
LOCUS       DACDEA010000247         4184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.133, whole genome shotgun sequence.
ACCESSION   DACDEA010000247 DACDEA010000000
VERSION     DACDEA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.133"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..1612
                     /gene="msr"
                     /locus_tag="IV136_002940"
     CDS             134..1612
                     /gene="msr"
                     /locus_tag="IV136_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8466828.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1659..2105
                     /locus_tag="IV136_002941"
     CDS             1659..2105
                     /locus_tag="IV136_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8466829.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2304..2819)
                     /locus_tag="IV136_002942"
     CDS             complement(2304..2819)
                     /locus_tag="IV136_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8466830.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2936..4138
                     /locus_tag="IV136_002943"
     CDS             2936..4138
                     /locus_tag="IV136_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8466831.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1243 a          707 c          910 g         1324 t
ORIGIN      
        1 atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt
       61 ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag
      121 gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg
      181 caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat
      241 cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt
      301 tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta
      361 tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt taagactgga
      421 agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct
      481 tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa
      541 aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag
      601 tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga
      661 aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa
      721 gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat
      781 ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca
      841 acaaatcaga ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat
      901 ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc
      961 gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta
     1021 tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg
     1081 tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa
     1141 gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt
     1201 aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg
     1261 ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt
     1321 agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac
     1381 ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc
     1441 gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc
     1501 gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt
     1561 ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta
     1621 cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag
     1681 gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt
     1741 tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg
     1801 tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta
     1861 aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg
     1921 ttttaggaat ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg
     1981 atcaattatt ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt
     2041 tgcagttgat catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg
     2101 aatgatggaa cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg
     2161 aatgcagttc tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc
     2221 tttttctttt ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt
     2281 tctagaaagg ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc
     2341 tgacaccatt ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt
     2401 ccaaattctt gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg
     2461 attttgttca gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc
     2521 gtcgaaaaag catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc
     2581 tctttgatcc attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt
     2641 tgagagtgct cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc
     2701 gttgtataga tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca
     2761 tacccagaac gtggtttgga taaaagcacg cataataata tataggctaa agtattcata
     2821 aaaatcctac ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac
     2881 atgtgcatat tgacatgtaa aataaatcat gtatgatgct aatagagaga aagggatgga
     2941 aatattggag aatcaaatag atatacaaga acaaacaaat gaagaaacaa aggggaaaag
     3001 ttcagcgcta gttgtagcag ctacctgtat gatcgctttt atgggagtgg gtctagtaga
     3061 cccaatctta aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt
     3121 atttactagt tacatgttag tcactggtgt ggtgatgcta tttacaggtt tcttatctag
     3181 tcggatcggt ttgaaaaaaa cattgatgac aggattgttt atcatcgttg tattcgctgg
     3241 tcttggcgga ttatctggga atatcggaat gctgattggg ttacgtgccg gctggggtgt
     3301 cgggaatgct ttatttgtct ctactgcttt agcaacgatc gtcagtattt tagttggtaa
     3361 cacagaaaaa gcaatcatga tgtatgaagc agcacttggc tgcggaatgg ctgttggccc
     3421 tctagtcggt ggggtcttgg gcagtggttc atggcgttat ccattttttg gggtagcatt
     3481 tttgatgttt tgcggtttta tagccctagc ggtactttta cctgaaactg agaaaccaaa
     3541 gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc aatcataaac ttcgttcagt
     3601 tggactaatc gcattgttat ataatttcgg attctttact ttacttgcgt atgcgccatt
     3661 tttattgatt ggctattctg aattagaagt cggttttgta ttttttggct ggggtgtgct
     3721 tctggctatt gcttcgattt tcgttgcacc ggctcttgag cggaagttta cgacttatcg
     3781 gacaatttta gcagcatttg tcttattcat tatttgtttg ctgttattag gagctggaac
     3841 ttctatgcca gtccttgtac cgatcagcat tgtgttagca ggatttttcc aaggaattat
     3901 caatacactg ttgacgacga ttgctatgga aataccaggt ctgcaacgaa acgttgcttc
     3961 gtccagctat agttttgtcc gctttttcgg tggagcttta gcaccattta ttgcaggtgt
     4021 gattggtgaa catattggtg aaagctatgc gttttatttt gcaggtgcca ttgttcttat
     4081 cagtgtatgt tgtatcattt atcaccgtaa ttattttgct acaaatgaag aagaataaga
     4141 aatcagaggt tgtgaaaaag ctgtcctgca tcaagtcata agaa
//
LOCUS       DACDEA010000248         2993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.149, whole genome shotgun sequence.
ACCESSION   DACDEA010000248 DACDEA010000000
VERSION     DACDEA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.149"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..188
                     /locus_tag="IV136_002944"
     CDS             3..188
                     /locus_tag="IV136_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8466832.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="IV136_002945"
     CDS             203..1072
                     /locus_tag="IV136_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8466833.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="IV136_002946"
     CDS             1053..1787
                     /locus_tag="IV136_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8466834.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="IV136_002947"
     CDS             1820..2728
                     /locus_tag="IV136_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8466835.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="IV136_002948"
     CDS             2725..2973
                     /locus_tag="IV136_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP8466836.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DACDEA010000249         1782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.179, whole genome shotgun sequence.
ACCESSION   DACDEA010000249 DACDEA010000000
VERSION     DACDEA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.179"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..148
                     /locus_tag="IV136_002949"
     CDS             <1..148
                     /locus_tag="IV136_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8466837.1"
                     /translation="ISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            160..1107
                     /locus_tag="IV136_002950"
     CDS             160..1107
                     /locus_tag="IV136_002950"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8466838.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1127..1654
                     /locus_tag="IV136_002951"
     CDS             1127..1654
                     /locus_tag="IV136_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8466839.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT          600 a          311 c          396 g          475 t
ORIGIN      
        1 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
       61 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
      121 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
      181 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
      241 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
      301 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
      361 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
      421 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
      481 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
      541 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
      601 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
      661 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
      721 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
      781 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
      841 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
      901 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
      961 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     1021 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     1081 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     1141 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     1201 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     1261 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     1321 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     1381 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     1441 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     1501 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     1561 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     1621 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     1681 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     1741 gtacataaaa cctgcaccta acataacaaa caaatgccaa ac
//
LOCUS       DACDEA010000250         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.193, whole genome shotgun sequence.
ACCESSION   DACDEA010000250 DACDEA010000000
VERSION     DACDEA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.193"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV136_002952"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV136_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV136_002953"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV136_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8466840.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDEA010000251          976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32318_2016
            SAMN12640259-rid9888463.guided.211, whole genome shotgun sequence.
ACCESSION   DACDEA010000251 DACDEA010000000
VERSION     DACDEA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640259
            Sequence Read Archive: SRR10041087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743031.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 18:57:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 18 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640259-rid9888463.guided.211"
                     /isolate="E32318_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            74..811
                     /gene="erm(B)"
                     /locus_tag="IV136_002954"
     CDS             74..811
                     /gene="erm(B)"
                     /locus_tag="IV136_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8466841.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          372 a          164 c          146 g          294 t
ORIGIN      
        1 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
       61 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
      121 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
      181 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
      241 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
      301 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
      361 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
      421 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
      481 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
      541 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
      601 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
      661 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
      721 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
      781 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
      841 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
      901 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
      961 tcttatcgat acaaat
//
